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https://www.readbyqxmd.com/read/27911350/accuracy-analysis-of-intrahepatic-fat-density-measurements-using-dual-energy-computed-tomography-validation-using-a-test-phantom
#1
Tomohiro Arai, Masaki Misawa, Miki Arai, Masafumi Shinozaki, Kayo Sakamoto, Yoshinobu Yajima, Yuichi Nozaki, Tsuyoshi Tajima, Masanori Sato, Fumihiko Hinoshita
BACKGROUND: Currently, no standardized method for measuring intrahepatic fat density via conventional computed tomography (CT) exists. OBJECTIVE: We aim to quantify intrahepatic fat density via material decomposition analysis using rapid kilovolt peak-switching dual-energy (RSDE) CT. METHODS: Homogenized porcine liver and fat (lard) were mixed in various ratios to produce phantoms for fat density verification. The actual fat density was measured on the basis of the phantom volume and weight, and these measurements were used as reference densities...
November 30, 2016: Journal of X-ray Science and Technology
https://www.readbyqxmd.com/read/27911265/mirna-543-promotes-osteosarcoma-cell-proliferation-and-glycolysis-by-partially-suppressing-prmt9-and-stabilizing-hif-1%C3%AE-protein
#2
Heng Zhang, Xiaofeng Guo, Xing Feng, Tingting Wang, Zhaohua Hu, Xiangyong Que, Qingsong Tian, Tianbo Zhu, Guixian Guo, Wei Huang, Xinzhi Li
Osteosarcoma (OS) is the most common primary bone tumor, occurring frequently in adolescents and possessing a high malignant severity. MicroRNAs play critical roles during OS development. Thus, elucidation of the involvement of specific microRNAs in the development of OS may provide novel therapeutic targets for OS treatment. Here, we showed that in the OS specimens from patients, the levels of miR-543 were significantly increased whereas the levels of PRMT9 were significantly decreased, compared to the paired normal bone tissue...
November 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27911133/changes-in-cervical-movement-impairment-and-pain-following-orofacial-treatment-in-patients-with-chronic-arthralgic-temporomandibular-disorder-with-pain-a-prospective-case-series
#3
Francis Grondin, Toby Hall
The purpose of this study was to investigate the influence of isolated temporomandibular joint (TMJ) manual therapy on pain and range of motion (ROM) of the TMJ and cervical spine including flexion-rotation test (FRT) in people suffering chronic pain arising from chronic arthralgic temporomandibular disorder (TMD). An experienced clinician managed a case series of 12 patients with TMD (mean duration 28.6 months +/- 26.9). The intervention comprised four-weekly sessions of transverse medial accessory TMJ mobilization and advice...
December 2, 2016: Physiotherapy Theory and Practice
https://www.readbyqxmd.com/read/27911110/mannose-binding-lectin-deficiency-in-brazilian-patients-with-spondyloarthritis
#4
Thelma L Skare, Renato Nisihara, Juliana Z Cieslinski, João O Zeni, Henrique N Rasera, Iara Messias-Reason, Shirley R R Utiyama
BACKGROUND: Infections are usually involved in the pathogenesis of spondyloarthritis (SpA). Mannose-binding lectin (MBL) is a component of the innate immune system with an important role in microbial defense. OBJECTIVE: To study the prevalence of MBL deficiency in SpA patients as well as its influence in the clinical profile of these diseases. METHODS: We studied 89 SpA patients and 89 healthy individuals, paired for age and gender. MBL serum levels were measured by ELISA test...
December 2, 2016: Immunological Investigations
https://www.readbyqxmd.com/read/27911049/family-partners-in-lifestyle-support-pals-family-based-weight-loss-for-african-american-adults-with-type-2-diabetes
#5
Carmen D Samuel-Hodge, Judith C Holder-Cooper, Ziya Gizlice, Gwendolyn Davis, Sonia P Steele, Thomas C Keyserling, Shiriki K Kumanyika, Phillip J Brantley, Laura P Svetkey
OBJECTIVE: To develop and test a family-centered behavioral weight loss intervention for African American adults with type 2 diabetes. METHODS: In this randomized trial, dyads consisting of an African American adult with overweight or obesity and type 2 diabetes (index participant) paired with a family partner with overweight or obesity but not diagnosed with diabetes were assigned in a 2:1 ratio to a 20-week special intervention (SI) or delayed intervention (DI) control group...
December 2, 2016: Obesity
https://www.readbyqxmd.com/read/27911048/fluorescent-oligonucleotide-probes-for-screening-high-affinity-nucleobase-surrogates
#6
Asmo Aro-Heinilä, Tuomas Lönnberg
Double-helical oligonucleotide probes featuring a single-nucleotide gap opposed by one of the canonical nucleobases and flanked by the fluorescent nucleobase analogue pyrrolocytosine have been synthesized and titrated with PdII chelates of dipicolinamide and its N2,N6-dialkylated derivatives. The fluorometric titrations revealed greatly increased affinity of the PdII chelates for the nucleobases opposing the gap compared to the respective free nucleotides in solution. Owing to the constrained environment of the single-nucleotide gap, the relative stabilities of the various PdII mediated base pairs were also significantly different from those previously reported at monomer level...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27911022/membrane-defects-and-genetic-redundancy-are-we-at-a-turning-point-for-dyt1-dystonia
#7
REVIEW
Ana Cascalho, Julie Jacquemyn, Rose E Goodchild
Heterozygosity for a 3-base pair deletion (ΔGAG) in TOR1A/torsinA is one of the most common causes of hereditary dystonia. In this review, we highlight current understanding of how this mutation causes disease from research spanning structural biochemistry, cell science, neurobiology, and several model organisms. We now know that homozygosity for ΔGAG has the same effects as Tor1a(KO) , implicating a partial loss of function mechanism in the ΔGAG/+ disease state. In addition, torsinA loss specifically affects neurons in mice, even though the gene is broadly expressed, apparently because of differential expression of homologous torsinB...
December 2, 2016: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/27910942/improved-bi-allelic-modification-of-a-transcriptionally-silent-locus-in-patient-derived-ipsc-by-cas9-nickase
#8
Reto Eggenschwiler, Mohsen Moslem, Mariane Serra Fráguas, Melanie Galla, Oliver Papp, Maximilian Naujock, Ines Fonfara, Ingrid Gensch, Annabell Wähner, Abbas Beh-Pajooh, Claudio Mussolino, Marcel Tauscher, Doris Steinemann, Florian Wegner, Susanne Petri, Axel Schambach, Emmanuelle Charpentier, Toni Cathomen, Tobias Cantz
Homology directed repair (HDR)-based genome editing via selectable long flanking arm donors can be hampered by local transgene silencing at transcriptionally silent loci. Here, we report efficient bi-allelic modification of a silent locus in patient-derived hiPSC by using Cas9 nickase and a silencing-resistant donor construct that contains an excisable selection/counter-selection cassette. To identify the most active single guide RNA (sgRNA)/nickase combinations, we employed a lentiviral vector-based reporter assay to determine the HDR efficiencies in cella...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910916/measuring-the-coefficient-of-friction-of-a-small-floating-liquid-marble
#9
Chin Hong Ooi, Anh Van Nguyen, Geoffrey M Evans, Dzung Viet Dao, Nam-Trung Nguyen
This paper investigates the friction coefficient of a moving liquid marble, a small liquid droplet coated with hydrophobic powder and floating on another liquid surface. A floating marble can easily move across water surface due to the low friction, allowing for the transport of aqueous solutions with minimal energy input. However, the motion of a floating marble has yet to be systematically characterised due to the lack of insight into key parameters such as the coefficient of friction between the floating marble and the carrier liquid...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910832/vacancy-pairing-and-superstructure-in-the-high-pressure-silicate-k1-5mg2si2o7h0-5-a-new-potential-host-for-potassium-in-the-deep-earth
#10
M D Welch, L Bindi, V Petříček, J Plášil
The high-pressure silicate K1.5Mg2Si2O7H0.5, synthesized and characterized by Welch et al. [(2012), Am. Mineral. 97, 1849-1857], has been re-examined with the aim of determining the nature of the superstructure noted in their study. The composition corresponds to a 1:1 combination of KMg2Si2O7H and K2Mg2Si2O7 end-members, but it is not a solid solution. Single-crystal X-ray diffraction data for one of the original K1.5Mg2Si2O7H0.5 crystals synthesized at 16 GPa/1573 K, has been collected using a much longer exposure time in order to improve the intensity statistics of weak superlattice reflections identified by Welch et al...
December 1, 2016: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/27910762/genetic-diversity-and-relationships-of-24-strains-of-genus-auricularia-agaricomycetes-assessed-using-srap-markers
#11
Ping Du, Tian-Xu Cao, Bao-Kai Cui, Yuan Yuan, Yu-Cheng Dai
Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Auricularia strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pairs, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910731/selective-attention-to-emotional-prosody-in-social-anxiety-a-dichotic-listening-study
#12
Virginie Peschard, Eva Gilboa-Schechtman, Pierre Philippot
The majority of evidence on social anxiety (SA)-linked attentional biases to threat comes from research using facial expressions. Emotions are, however, communicated through other channels, such as voice. Despite its importance in the interpretation of social cues, emotional prosody processing in SA has been barely explored. This study investigated whether SA is associated with enhanced processing of task-irrelevant angry prosody. Fifty-three participants with high and low SA performed a dichotic listening task in which pairs of male/female voices were presented, one to each ear, with either the same or different prosody (neutral or angry)...
December 2, 2016: Cognition & Emotion
https://www.readbyqxmd.com/read/27910674/control-of-secondary-electrons-from-ion-beam-impact-using-a-positive-potential-electrode
#13
T P Crowley, D R Demers, P J Fimognari
Secondary electrons emitted when an ion beam impacts a detector can amplify the ion beam signal, but also introduce errors if electrons from one detector propagate to another. A potassium ion beam and a detector comprised of ten impact wires, four split-plates, and a pair of biased electrodes were used to demonstrate that a low-voltage, positive electrode can be used to maintain the beneficial amplification effect while greatly reducing the error introduced from the electrons traveling between detector elements...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910673/implementation-of-the-new-multichannel-x-mode-edge-density-profile-reflectometer-for-the-icrf-antenna-on-asdex-upgrade
#14
D E Aguiam, A Silva, V Bobkov, P J Carvalho, P F Carvalho, R Cavazzana, G D Conway, O D'Arcangelo, L Fattorini, H Faugel, A Fernandes, H Fünfgelder, B Gonçalves, L Guimarais, G De Masi, L Meneses, J M Noterdaeme, R C Pereira, G Rocchi, J M Santos, A A Tuccillo, O Tudisco
A new multichannel frequency modulated continuous-wave reflectometry diagnostic has been successfully installed and commissioned on ASDEX Upgrade to measure the plasma edge electron density profile evolution in front of the Ion Cyclotron Range of Frequencies (ICRF) antenna. The design of the new three-strap ICRF antenna integrates ten pairs (sending and receiving) of microwave reflectometry antennas. The multichannel reflectometer can use three of these to measure the edge electron density profiles up to 2 × 10(19) m(-3), at different poloidal locations, allowing the direct study of the local plasma layers in front of the ICRF antenna...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910669/end-loss-analyzer-system-for-measurements-of-plasma-flux-at-the-c-2u-divertor-electrode
#15
M E Griswold, S Korepanov, M C Thompson
An end loss analyzer system consisting of electrostatic, gridded retarding-potential analyzers and pyroelectric crystal bolometers was developed to characterize the plasma loss along open field lines to the divertors of C-2U. The system measures the current and energy distribution of escaping ions as well as the total power flux to enable calculation of the energy lost per escaping electron/ion pair. Special care was taken in the construction of the analyzer elements so that they can be directly mounted to the divertor electrode...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910646/a-streaked-x-ray-spectroscopy-platform-for-rapidly-heated-near-solid-density-plasmas
#16
C R Stillman, P M Nilson, S T Ivancic, C Mileham, I A Begishev, R K Junquist, D J Nelson, D H Froula
A picosecond, time-resolved, x-ray spectroscopy platform was developed to study the thermal line emission from rapidly heated solid targets containing buried aluminum or iron layers. The targets were driven by high-contrast 1ω or 2ω laser pulses at focused intensities up to 1 × 10(19) W/cm(2). The experimental platform combines time-integrating and time-resolved x-ray spectrometers. Picosecond time resolution was achieved with a pair of ultrafast x-ray streak cameras coupled to high-throughput Hall spectrometers...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910631/a-simple-approach-to-spectrally-resolved-fluorescence-and-bright-field-microscopy-over-select-regions-of-interest
#17
Peter D Dahlberg, Christopher T Boughter, Nabil F Faruk, Lu Hong, Young Hoon Koh, Matthew A Reyer, Alon Shaiber, Aiman Sherani, Jiacheng Zhang, Justin E Jureller, Adam T Hammond
A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910516/plasma-rotation-in-the-peking-university-plasma-test-device
#18
Chijie Xiao, Yihang Chen, Xiaoyi Yang, Tianchao Xu, Long Wang, Min Xu, Dong Guo, Yi Yu, Chen Lin
Some preliminary results of plasma rotations in a linear plasma experiment device, Peking University Plasma Test (PPT) device, are reported in this paper. PPT has a cylindrical vacuum chamber with 500 mm diameter and 1000 mm length, and a pair of Helmholtz coils which can generate cylindrical or cusp magnetic geometry with magnitude from 0 to 2000 G. Plasma was generated by a helicon source and the typical density is about 10(13) cm(-3) for the argon plasma. Some Langmuir probes, magnetic probes, and one high-speed camera are set up to diagnose the rotational plasmas...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910478/measurement-of-turbulent-electron-temperature-fluctuations-on-the-asdex-upgrade-tokamak-using-correlated-electron-cyclotron-emission
#19
S J Freethy, G D Conway, I Classen, A J Creely, T Happel, A Köhn, B Vanovac, A E White
Turbulent temperature fluctuations are measured on the ASDEX Upgrade tokamak using pairs of closely spaced, narrow-band heterodyne radiometer channels and a standard correlation technique. The pre-detection spacing and bandwidth of the radiometer channel pairs is chosen such that they are physically separated less than a turbulent correlation length, but do not overlap. The radiometer has 4 fixed filter frequency channels and two tunable filter channels for added flexibility in the measurement position. Relative temperature fluctuation amplitudes are observed in a helium plasma to be δT/T = (0...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910460/low-magnetic-johnson-noise-electric-field-plates-for-precision-measurement
#20
I M Rabey, J A Devlin, E A Hinds, B E Sauer
We describe a parallel pair of high voltage electric field plates designed and constructed to minimise magnetic Johnson noise. They are formed by laminating glass substrates with a commercially available polyimide (Kapton) tape, covered with a thin gold film. Tested in vacuum, the outgassing rate is less than 5 × 10(-5) mbar l/s. The plates have been operated at electric fields up to 8.3 kV/cm, when the leakage current is at most a few hundred pA. The design is discussed in the context of a molecular spin precession experiment to measure the permanent electric dipole moment of the electron...
November 2016: Review of Scientific Instruments
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