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https://www.readbyqxmd.com/read/29150937/cgmp-production-and-analysis-of-bg505-sosip-664-an-extensively-glycosylated-trimeric-hiv-1-envelope-glycoprotein-vaccine-candidate
#1
Antu K Dey, Albert Cupo, Gabriel Ozorowski, Vaneet K Sharma, Anna-Janina Behrens, Eden P Go, Thomas J Ketas, Anila Yasmeen, Per J Klasse, Eddy Sayeed, Heather Desaire, Max Crispin, Ian A Wilson, Rogier W Sanders, Thomas Hassell, Andrew Ward, John P Moore
We describe the properties of BG505 SOSIP.664 HIV-1 envelope glycoprotein trimers produced under current Good Manufacturing Practice (cGMP) conditions. These proteins are the first of a new generation of native-like trimers that are the basis for many structure-guided immunogen development programs aimed at devising how to induce broadly neutralizing antibodies (bNAbs) to HIV-1 by vaccination. The successful translation of this prototype demonstrates the feasibility of producing similar immunogens on an appropriate scale and of an acceptable quality for Phase I experimental medicine clinical trials...
November 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29150928/application-of-high-speed-counter-current-chromatography-and-hplc-to-separate-and-purify-of-three-polyacetylenes-from-platycodon-grandiflorum
#2
Bao Chen, Zhengbo Liu, Yuwei Zhang, Wei Li, Yanjun Sun, Yufang Wang, Yanhua Wang, Yinshi Sun
Three polyacetylenes were isolated and purified from Platycodon grandiflorum A. DC for the first time by high-speed counter-current chromatography using a two-phase solvent system composed of hexane/ethyl acetate/methanol/water (1:31:1:31, v/v/v/v) and high-performance liquid chromatography with an Agilent ZORBAX(®) SB-C18 column (4.6 mm × 150 mm, 5 μm). After separation by high-speed counter-current chromatography and high-performance liquid chromatography, we obtained 3.5 mg of platetyolin A, 4.1 mg of platetyolin B, and 18...
November 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29150919/a-focus-on-the-beneficial-effects-of-alpha-synuclein-and-a-re-appraisal-of-synucleinopathies
#3
Larisa Ryskalin, Carla L Busceti, Fiona Limanaqi, Francesca Biagioni, Stefano Gambardella, Francesco Fornai
Alpha synuclein (α-syn) belongs to a class of proteins which are commonly considered to play a detrimental role in neuronal survival. This assumption is based on the occurrence of a severe neuronal degeneration in patients carrying a multiplication of the α-syn gene (SNCA) and in a variety of experimental models, where overexpression of α-syn leads to cell death and neurological impairment. In these conditions, a higher amount of normally structured α-syn produces a damage, which is even worse compared with that produced by α-syn owing an abnormal structure (as occurring following point gene mutations)...
November 16, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/29150793/high-efficient-liquid-exfoliation-of-boron-nitride-nanosheets-using-aqueous-solution-of-alkanolamine
#4
Bangwen Zhang, Qian Wu, Huitao Yu, Chaoke Bulin, He Sun, Ruihong Li, Xin Ge, Ruiguang Xing
As one of the simple and efficient routes to access two-dimensional materials, liquid exfoliation has received considerable interest in recent years. Here, we reported on high-efficient liquid exfoliation of hexagonal boron nitride nanosheets (BNNSs) using monoethanolamine (MEA) aqueous solution. The resulting BNNSs were evaluated in terms of the yield and structure characterizations. The results show that the MEA solution can exfoliate BNNSs more efficiently than the currently known solvents and a high yield up to 42% is obtained by ultrasonic exfoliation in MEA-30 wt% H2O solution...
November 17, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29150719/preoperative-optimization-for-major-hepatic-resection
#5
REVIEW
Sarah Walcott-Sapp, Kevin G Billingsley
PURPOSE: Major hepatic resections are performed for primary hepatobiliary malignancies, metastatic disease, and benign lesions. Patients with chronic liver disease, including cirrhosis and steatosis, are at an elevated risk of malnutrition and impaired strength and exercise capacity, deficits which cause increased risk of postoperative complications and mortality. The aims of this report are to discuss the pathophysiology of changes in nutrition, exercise capacity, and muscle strength in patient populations likely to require major hepatectomy, and review recommendations for preoperative evaluation and optimization...
November 18, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/29150689/distinct-genetic-architectures-for-phenotype-means-and-plasticities-in-zea-mays
#6
Aaron Kusmec, Srikant Srinivasan, Dan Nettleton, Patrick S Schnable
Phenotypic plasticity describes the phenotypic variation of a trait when a genotype is exposed to different environments. Understanding the genetic control of phenotypic plasticity in crops such as maize is of paramount importance for maintaining and increasing yields in a world experiencing climate change. Here, we report the results of genome-wide association analyses of multiple phenotypes and two measures of phenotypic plasticity in a maize nested association mapping (US-NAM) population grown in multiple environments and genotyped with ~2...
September 2017: Nature Plants
https://www.readbyqxmd.com/read/29150653/a-theoretical-quest-for-high-temperature-superconductivity-on-the-example-of-low-dimensional-carbon-structures
#7
C H Wong, R Lortz, E A Buntov, R E Kasimova, A F Zatsepin
High temperature superconductivity does not necessarily require correlated electron systems with complex competing or coexisting orders. Instead, it may be achieved in a phonon-mediated classical superconductor having a high Debye temperature and large electronic density of states at the Fermi level in a material with light atoms and strong covalent bonds. Quasi-1D conductors seem promising due to the Van Hove singularities in their electronic density of states. In this sense, quasi-1D carbon structures are good candidates...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150558/branched-pectic-galactan-in-phloem-sieve-element-cell-walls-implications-for-cell-mechanics
#8
Thomas A Torode, Rachel E O'Neill, Susan E Marcus, Valerie Cornuault, Sara Pose-Albacete, Rebecca P Lauder, Stjepan Kresimir Kracun, Maja Gro Rydahl, Mathias C F Andersen, William George Tycho Willats, Siobhan Ariel Braybrook, Belinda J Townsend, Mads H Clausen, J Paul Knox
A major question in plant biology concerns the specification and functional differentiation of cell types. This is in the context of constraints imposed by networks of cell walls that both adhere cells and contribute to the form and function of developing organs. Here, we report the identification of a glycan epitope that is specific to phloem sieve element cell walls in several systems. A monoclonal antibody, designated LM26, binds to the cell wall of phloem sieve elements in stems of Arabidopsis thaliana, Miscanthus x giganteus and notably sugar beet (Beta vulgaris) roots where phloem identification is an important factor for the study of phloem unloading of sucrose...
November 17, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29150509/the-tape-measure-protein-is-involved-in-the-heat-stability-of-lactococcus-lactis-phages
#9
Hany Geagea, Simon J Labrie, Muriel Subirade, Sylvain Moineau
Virulent lactococcal phages are still a major risk for milk fermentation processes as they may lead to slowdowns and low quality fermented dairy products, particularly cheeses. Some of the phage control strategies used by the industry rely on heat treatments. Recently, a few Lactococcus lactis phages were found to be highly thermo-resistant. To identify the genetic determinant(s) responsible for the thermal resistance of lactococcal phages, we used the virulent phage CB14 (sk1virus/936 group) to select for phage mutants with increased heat stability...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150506/the-swinholide-biosynthetic-gene-cluster-from-a-terrestrial-cyanobacterium-nostoc-sp-uhcc-0450
#10
Anu Humisto, Jouni Jokela, Liwei Liu, Matti Wahlsten, Hao Wang, Perttu Permi, João Paulo Machado, Agostinho Antunes, David P Fewer, Kaarina Sivonen
Swinholides are 42-carbon ring polyketides with a twofold axis of symmetry. They are potent cytotoxins that disrupt the actin cytoskeleton. Swinholides were discovered from the marine sponge Theonella sp. and were long-suspected to be produced by symbiotic bacteria. Misakinolide, a structural variant of swinholide, was recently demonstrated to be the product of the symbiotic heterotrophic proteobacterium. Here we report the production of swinholide A by an axenic strain of the terrestrial cyanobacterium Nostoc sp...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150353/barrier-dysfunction-in-the-nasal-allergy
#11
REVIEW
Ayumi Fukuoka, Tomohiro Yoshimoto
Epithelial cells form the first physiological barrier against invasion by pathogens and the infiltration of allergens. Tight junctions (TJ), a cell-cell junctional complex located on the apical side of epithelial cells, have a critical role in the maintenance of epithelial barrier function. Impaired TJ structures are observed in patients with asthma, atopic dermatitis and nasal allergy; therefore, the dysfunction of epithelial barriers might be involved in the initiation or progression of allergic diseases...
November 14, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/29150298/spontaneous-osteoclastogenesis-hypothesis-for-gender-unrelated-osteoporosis-screening-and-diagnosis
#12
F Salamanna, R Giardino, M Fini
Women are at greater risk of developing osteoporosis (OP). However, in the past few years it has become more widely recognized that OP is a significant problem also in men although OP is frequently under-diagnosed and, consequently, under treated in men. Most guidelines, screening and fracture risk evaluation methods as well as pharmacologic agents have been developed for women and then adapted to men. Bone Mineral Density (BMD) measurement by Dual X-ray Absorptiometry (DEXA) is reported as T score and the capability of DEXA to diagnose OP and predict fracture risk is still debated...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29150297/prolonged-stretching-of-the-ankle-plantarflexors-elicits-muscle-tendon-adaptations-relevant-to-ankle-gait-kinetics-in-children-with-spastic-cerebral-palsy
#13
Teresa Martín Lorenzo, Eduardo Rocon, Ignacio Martínez Caballero, Ana Ramírez Barragán, Sergio Lerma Lara
Tissue related ankle hyper-resistance has been reported to contribute to equinus gait in children with spastic cerebral palsy. Hence, ankle plantarflexor stretching programs have been developed in order to restore passive ankle dorsiflexion. Despite high quality evidence on the limited effects of stretching on passive joint mobility, further muscle-tendon adaptations have been reported which may impact gait performance. As such, children with spastic cerebral palsy subject to long-term manual static stretching achieved dorsiflexion gains through the reduction of muscle and fascicle strain whilst preserving tendon strain, and prolonged use of ankle-foot orthoses achieved similar dorsiflexion gains through increased tendon strain whilst preserving muscle and fascicle strain...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29150251/validity-of-birth-certificate-and-hospital-discharge-data-reporting-of-labor-induction
#14
Kristen H Kjerulff, Laura B Attanasio
PURPOSE: To examine the concordance of labor induction measures derived from birth certificate and hospital discharge data with each other and with maternal report. METHODS: Birth certificate data were linked with hospital discharge data and structured interviews of 2,851 mothers conducted 1 month after first childbirth. Those who reported that a doctor or nurse tried to cause their labor to begin, and were not in labor before that event, were classified as undergoing labor induction...
November 14, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/29150228/surgeons-perceptions-of-the-causes-of-preventable-harm-in-arterial-surgery-a-mixed-methods-study
#15
Rachael Lear, Anthony D Godfrey, Celia Riga, Christine Norton, Charles Vincent, Colin D Bicknell
BACKGROUND: System factors contributing to preventable harm in vascular patients have not been previously reported in detail. The aim of this exploratory mixed-methods study was to describe vascular surgeons' perceptions of factors contributing to adverse events (AEs) in arterial surgery. A secondary aim was to report recommendations to improve patient safety. METHODS: Vascular consultants/registrars working in the British National Health Service were questioned about the causes of preventable AEs through survey and semi-structured interview (response rates 77% and 83%, respectively)...
November 15, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29150196/single-stage-urethroplasty-with-buccal-mucosal-inlay-graft-for-stricture-caused-by-balanitis-xerotica-obliterans-in-boys-outcomes-in-the-medium-term
#16
Junaid Ashraf, Alexander Turner, Ramnath Subramaniam
INTRODUCTION: Balanitis xerotica obliterans (BXO) is a common condition that can affect the foreskin, glans, meatus, and urethra, and rarely can also involve urethra leading to stricture. Numerous procedures have been suggested to treat urethral stricture secondary to BXO but these have had variable results. OBJECTIVE: We describe the first prospective study of a single stage buccal mucosal inlay grafting in children with urethral strictures resistant to existing remedies...
October 31, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29150150/biocompatible-hollow-polymeric-particles-produced-by-a-mild-solvent-and-template-free-strategy
#17
Eustolia Rodríguez-Velázquez, Pablo Taboada, Manuel Alatorre-Meda
Macroscopic hollow polymeric particles are attractive materials for various applications such as surgery, food industry, agriculture, etc. However, protocols reporting their synthesis have hitherto made use of organic solvents and/or sacrificial templates, compromising the encapsulation of different bioactive compounds and the process yield. Here, millimeter-size, hollow polymeric particles were synthesized, for the first time, in a solvent- and template free manner onto superhydrophobic surfaces (SHS). The particles were produced upon assembly and double superficial crosslinking of liquid droplets of DNA and methacrylamide chitosan aqueous solutions (CH:MA), leading to liquid-core particles with a hardened hydrogel shell...
November 14, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29150132/prosthodontic-rehabilitation-with-monolithic-multichromatic-cad-cam-complete-overdentures-in-an-adolescent-patient-with-ectodermal-dysplasia-a-clinical-report
#18
Hussain D Alsayed, Nasser M Alqahtani, Yasmin M Alzayer, Dean Morton, John A Levon, Nadim Z Baba
Ectodermal dysplasia is a rare, hereditary, congenital disease that affects the normal development of certain tissues and structures of ectodermal origin. The disease is manifested to different degrees of severity and may involve the nose, eyes, hair, nails, sweat glands, and enamel. This report describes a 14-year-old boy with ectodermal dysplasia, rehabilitated with monolithic, multichromatic maxillary and mandibular computer-aided design and computer-aided manufacturing (CAD-CAM) acrylic resin complete overdentures...
November 15, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/29150077/unique-arginine-array-improves-cytosolic-localization-of-hydrocarbon-stapled-peptides
#19
Kim Quach, Jonathan LaRochelle, Xiao-Han Li, Elizabeth Rhoades, Alanna Schepartz
We have previously reported that miniature proteins containing a distinct array of 5 arginine residues on a folded α-helix - a penta-arg motif - traffic with high efficiency from endosomes into the cytosol and nucleus of mammalian cells. Here we evaluate whether a penta-arg motif can improve the intracellular trafficking of an otherwise impermeant hydrocarbon-stapled peptide, SAH-p53-4(Rho). We prepared a panel of SAH-p53-4(Rho) variants containing penta-arg sequences with different spacings and axial arrangement and evaluated their overall uptake (as judged by flow cytometry) and their intracellular access (as determined by fluorescence correlation spectroscopy, FCS)...
November 7, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29149989/low-background-and-visual-detection-of-antibiotic-based-on-target-activated-colorimetric-split-peroxidase-dnazyme-coupled-with-dual-nicking-enzyme-signal-amplification
#20
REVIEW
Xuejun Cui, Rongguo Li, Xiaofei Liu, Jingfeng Wang, Xueqi Leng, Xiaolei Song, Qianqian Pei, Yu Wang, Su Liu, Jiadong Huang
Herein, we have reported the development of a simple, rapid, and low cost colorimetric method for the detection of antibiotic based on target-activated split peroxidase DNAzyme coupled with dual nicking enzyme signal amplification (NESA). To lower background signal in G-quadruplex DNAzyme-based detection, the two split G-rich parts are caged into two different hairpin probes, respectively, preventing the two parts from assembling into the G-quadruplex structure. By the combination of restriction endonuclease-assisted cleavage reaction with the spilt G-quadruplex probes, target-modulated release of the two split G-rich parts is achieved, affording high specificity of antibiotic detection...
January 2, 2018: Analytica Chimica Acta
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