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https://www.readbyqxmd.com/read/28239975/preparing-to-read-the-ubiquitin-code-top-down-analysis-of-unanchored-ubiquitin-tetramers
#1
Amanda E Lee, Lucia Geis-Asteggiante, Emma K Dixon, Meredith Miller, Yan Wang, David Fushman, Catherine Fenselau
The characterization of polyubiquitin chains has been an analytical challenge for several decades. It has been shown that anchored and unanchored polyubiquitin chains with different isopeptide linkages and lengths exhibit a wide range of profoundly different cellular functions. However, structure function studies have been hindered by the difficulty of characterizing these complex chain structures. This report presents a broadly applicable workflow to characterize ubiquitin tetramers without the need for genetic mutations or reiterative immunoprecipitations...
August 2016: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28239963/characterization-of-complex-heterogeneous-lipid-a-samples-using-hplc-ms-ms-technique-ii-structural-elucidation-of-non-phosphorylated-lipid-a-by-negative-ion-mode-tandem-mass-spectrometry
#2
Viktor Sándor, Anikó Kilár, Ferenc Kilár, Béla Kocsis, Ágnes Dörnyei
Non-phosphorylated lipid A species confer reduced inflammatory potential for the bacteria. Knowledge on their chemical structure and presence in bacterial pathogens may contribute to the understanding of bacterial resistance and activation of the host innate immune system. In this study, we report the fragmentation pathways of negatively charged, non-phosphorylated lipid A species under low-energy collision-induced dissociation conditions of an electrospray ionization quadrupole time-of-flight instrument. Charge-promoted consecutive and competitive eliminations of the acyl chains and cross-ring cleavages of the sugar residues were observed...
August 2016: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28239758/acellular-mouse-kidney-ecm-can-be-used-as-a-three-dimensional-substrate-to-test-the-differentiation-potential-of-embryonic-stem-cell-derived-renal-progenitors
#3
Manpreet Sambi, Theresa Chow, Jennifer Whiteley, Mira Li, Shawn Chua, Vanessa Raileanu, Ian M Rogers
The development of strategies for tissue regeneration and bio-artificial organ development is based on our understanding of embryogenesis. Differentiation protocols attempt to recapitulate the signaling modalities of gastrulation and organogenesis, coupled with cell selection regimens to isolate the cells of choice. This strategy is impeded by the lack of optimal in vitro culture systems since traditional culture systems do not allow for the three-dimensional interaction between cells and the extracellular matrix...
February 27, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28239735/monte-carlo-simulation-of-magnetic-domain-structure-and-magnetic-properties-near-the-morphotropic-phase-boundary
#4
Songrui Wei, Sen Yang, Dong Wang, Xiaoping Song, Xiaoqin Ke, Yipeng Gao, Xiaoqi Liao, Yunzhi Wang
The morphotropic phase boundary (MPB), which is the boundary separating a tetragonal phase from a rhombohedral phase by varying the composition or mechanical pressure in ferroelectrics, has been studied extensively for decades because it can lead to strong enhancement of piezoelectricity. Recently, a parallel ferromagnetic MPB was experimentally reported in the TbCo2-DyCo2 ferromagnetic system and this discovery proposes a new way to develop potential materials with giant magnetostriction. However, the role of magnetic domain switching and spin reorientation near the MPB region is still unclear...
February 27, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28239724/inner-to-outer-sphere-coordination-of-plutonium-iv-with-n-n-dialkyl-amide-influence-of-nitric-acid
#5
Eléonor Acher, Thomas Dumas, Christelle Tamain, Nathalie Boubals, Pier Lorenzo Solari, Dominique Guillaumont
N,N-Dialkylamides are extensively studied as alternative organic ligands to achieve the extraction and separation of uranium(vi) and plutonium(iv). We report here the coordination structures of the plutonium(iv) ion with N,N-di(2-ethylhexyl)-n-butanamide as a function of nitric acid concentration in the aqueous phase. The coordination structure of Pu(iv) evolves gradually with increasing nitric acid concentration from an inner-sphere with two coordinated amide ligands toward an outer-sphere hexanitrate complex with only nitrate ions in the first coordination sphere and protonated amide ligands in the outer shell...
February 27, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28239518/childhood-maltreatment-psychopathology-and-the-development-of-hippocampal-subregions-during-adolescence
#6
Sarah Whittle, Julian G Simmons, Sylke Hendriksma, Nandita Vijayakumar, Michelle L Byrne, Meg Dennison, Nicholas B Allen
INTRODUCTION: It is well established that childhood maltreatment has a detrimental impact on the brain, particularly the hippocampus. However, the hippocampus is a functionally and structurally heterogeneous region, and little is known about how maltreatment might affect hippocampal subregion development throughout important periods of plasticity. This study investigated whether childhood maltreatment was associated with the development of hippocampal subregion volumes from early to late adolescence...
February 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28239485/protocol-for-the-evaluation-of-a-digital-storytelling-approach-to-address-stigma-and-improve-readiness-to-seek-services-among-veterans
#7
Brian E Bunnell, Tatiana M Davidson, Jessica L Hamblen, Danna L Cook, Anouk L Grubaugh, Brian E Lozano, Peter W Tuerk, Kenneth J Ruggiero
BACKGROUND: Research suggests that at least 10% of veterans returning from Iraq and Afghanistan meet criteria for posttraumatic stress disorder (PTSD) related to their military experiences. National dissemination initiatives have increased veterans' access to best-practice interventions. However, treatment-seeking remains low among veterans with PTSD, often due to perceived stigma and other associated barriers. The National Center for PTSD recently developed and launched AboutFace, a digital storytelling (DST) resource designed to help veterans recognize PTSD and motivate them to seek evidence-based treatment...
2017: Pilot and Feasibility Studies
https://www.readbyqxmd.com/read/28239477/stimuli-responsive-furan-and-thiophene-substituted-difluoroboron-%C3%AE-diketonate-materials
#8
William A Morris, Tristan Butler, Milena Kolpaczynska, Cassandra L Fraser
Difluoroboron β-diketonate (BF2bdk) compounds exhibit solid-state switchable luminescence under excitation by UV light. This property is usually manifested as a blue-shift in emission when dye films are thermally annealed followed by a red-shift in response to mechanical shear (i.e. mechanochromic luminescence). Here we report thiophene and furan heterocycle-substituted dyes bearing short methoxy and long dodecyloxy chain substituents. Optical properties of the dyes were investigated in solution, pristine powders, and films on paper and glass...
January 1, 2017: Mater Chem Front
https://www.readbyqxmd.com/read/28239342/informative-cues-facilitate-saccadic-localization-in-blindsight-monkeys
#9
Masatoshi Yoshida, Ziad M Hafed, Tadashi Isa
Patients with damage to the primary visual cortex (V1) demonstrate residual visual performance during laboratory tasks despite denying having a conscious percept. The mechanisms behind such performance, often called blindsight, are not fully understood, but the use of surgically-induced unilateral V1 lesions in macaque monkeys provides a useful animal model for exploring such mechanisms. For example, V1-lesioned monkeys localize stimuli in a forced-choice condition while at the same time failing to report awareness of identical stimuli in a yes-no detection condition, similar to human patients...
2017: Frontiers in Systems Neuroscience
https://www.readbyqxmd.com/read/28239244/effect-of-trajectories-of-friends-and-parents-school-involvement-on-adolescents-engagement-and-achievement
#10
Myung Hee Im, Jan N Hughes, Stephen G West
In a sample of 527 academically at-risk youth, we investigated trajectories of friends' and parents' school involvement across ages 12-14 and the joint contributions of these trajectories to adolescents' age 15 school engagement and academic achievement. Girls reported higher levels of friends' and parents' school involvement than boys. Both parents' and friends' school involvement declined across ages 12-14. Combined latent growth models and structural equation models showed effects of the trajectories of friends' and parents' school involvement on adolescents' age 15 school engagement and academic achievement, over and above adolescents' prior performance...
December 2016: Journal of Research on Adolescence: the Official Journal of the Society for Research on Adolescence
https://www.readbyqxmd.com/read/28239215/the-techniques-for-overcoming-depression-questionnaire-mokken-scale-analysis-reliability-and-concurrent-validity-in-depressed-cardiac-patients
#11
Kenneth E Freedland, Mariantonia Lemos, Frank Doyle, Brian C Steinmeyer, Iris Csik, Robert M Carney
OBJECTIVES: The Techniques for Overcoming Depression (TOD) questionnaire assesses the frequency with which patients being treated for depression use cognitive-behavioral techniques in daily life. This study examined its latent structure, reliability and concurrent validity in depressed cardiac patients. METHOD: The TOD was administered at the initial and final treatment sessions in three trials of cognitive behavior therapy (CBT) (n = 260) for depression in cardiac patients...
February 2017: Cognitive Therapy and Research
https://www.readbyqxmd.com/read/28239158/synthesis-of-the-novel-parp-1-inhibitor-ag-690-11026014-and-its-protective-effects-on-angiotensin-ii-induced-mouse-cardiac-remodeling
#12
Guo-Shuai Feng, Cui-Ge Zhu, Zhuo-Ming Li, Pan-Xia Wang, Yi Huang, Min Liu, Ping He, Lan-Lan Lou, Shao-Rui Chen, Pei-Qing Liu
We previously identified AG-690/11026014 (6014) as a novel poly(ADP-ribose) polymerase-1 (PARP-1) inhibitor that effectively prevented angiotensin II (Ang II)-induced cardiomyocyte hypertrophy. In the present study, we reported a new synthesis route for 6014, and investigated its protective effects on Ang II-induced cardiac remodeling and cardiac dysfunction and the underlying mechanisms in mice. We designed a new synthesis route to obtain a sufficient quantity of 6014 for this in vivo study. C57BL/6J mice were infused with Ang II and treated with 6014 (10, 30, 90 mg·kg(-1)·d(-1), ig) for 4 weeks...
February 27, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28239056/thermodynamic-analysis-of-the-transition-of-liquid-crystals-from-lamellar-to-vesicular-phase
#13
Yasutaka Enomoto, Yoko Imai, Kazuo Tajima
Here, we report the results of thermodynamic analyses on the lamellar-vesicular transition for a cationic amphiphilic species, namely 2-hydroxyethyl di(alkanol)oxyethyl methylammonium methylsulfate (DEAE). Previously, we have shown that spontaneous vesicle formation from a Lα-lamellar liquid crystal (LC) phase only occurs on the addition of a quantitative amount of additives to the DEAE LC at certain temperatures and that this change occurs without the input of any extra mechanical energy. These lamellar-vesicular transitions occur in two steps: the first step is the formation of an excited state, caused by the solubilization of organic substances in the bilayer structure...
February 24, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28238889/on-the-relation-between-pitch-and-level
#14
Yi Zheng, Romain Brette
Pitch is the perceptual dimension along which musical notes are ordered from low to high. It is often described as the perceptual correlate of the periodicity of the sound's waveform. Previous reports have shown that pitch can depend slightly on sound level. We wanted to verify that these observations reflect genuine changes in perceived pitch, and were not due to procedural factors or confusion between dimensions of pitch and level. We first conducted a systematic pitch matching task and confirmed that the pitch of low frequency pure tones, but not complex tones, decreases by an amount equivalent to a change in frequency of more than half a semitone when level increases...
February 23, 2017: Hearing Research
https://www.readbyqxmd.com/read/28238880/analysis-on-the-expression-and-function-of-a-chicken-type-and-goose-type-lysozymes-in-chinese-giant-salamanders-andrias-davidianus
#15
Hui Yang, Ranran Liu, Dan Cui, Haixia Liu, Dongmei Xiong, Xiaolin Liu, Lixin Wang
Lysozymes as an important immune factor, play vital roles in innate immune response against pathogen infection. In the present study, one c-type and g-type lysozymes were identified from Chinese giant salamander (Andrias davidianus). They shared highly conserved structural features with lysozymes from other species. Spatial expression analysis revealed that AdlysC transcript was most abundant in liver and stomach, and least in muscle and brain. In contrast, the expression level of AdlysG was most abundant in liver and least in muscle and skin...
February 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28238801/chromosomal-location-of-the-fosa3-and-blactx-m-genes-in-proteus-mirabilis-and-clonal-spread-of-escherichia-coli-st117-carrying-fosa3-positive-inchi2-st3-or-f2-a-b-plasmids-in-a-chicken-farm
#16
Dandan He, Lanping Liu, Baowei Guo, Shengjun Wu, Xiaojie Chen, Jing Wang, Zhenling Zeng, Jian-Hua Liu
The aim of this study was to investigate the spread and location of the fosA3 gene among Enterobacteriaceae from diseased broiler chickens. Twenty-nine Escherichia coli and seven Proteus mirabilis isolates recovered from one chicken farm were screened for the presence of plasmid-mediated fosfomycin resistance genes by PCR. The clonal relatedness of fosA3-positive isolates, the transferability and location of fosA3, and the genetic context of the fosA3 gene were determined. Seven P. mirabilis isolates with three different pulsed-field gel electrophoresis (PFGE) patterns and five E...
February 23, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28238763/structure-and-function-of-a-novel-atpase-that-interacts-with-holliday-junction-resolvase-hjc-and-promotes-branch-migration
#17
Binyuan Zhai, Kevin DuPrez, Tzanko I Doukov, Huan Li, Mengting Huang, Guijun Shang, Jinfeng Ni, Lichuan Gu, Yulong Shen, Li Fan
Holliday junction (HJ) is a hallmark intermediate in DNA recombination and must be processed by dissolution (for double HJ) or resolution to ensure genome stability. Although HJ resolvases have been identified in all domains of life, there is a long-standing effort to search in prokaryotes and eukarya for proteins promoting HJ migration. Here we report the structural and functional characterization of a novel ATPase, Sulfolobus islandicusPIN-domain containing ATPase (SisPINA), encoded by the gene adjacent to the resolvase Hjc coding gene...
February 23, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28238661/motor-activity-dependent-and-independent-functions-of-myosin-ii-contribute-to-actomyosin-ring-assembly-and-contraction-in-schizosaccharomyces-pombe
#18
Saravanan Palani, Ting Gang Chew, Srinivasan Ramanujam, Anton Kamnev, Shrikant Harne, Bernardo Chapa-Y-Lazo, Rebecca Hogg, Mayalagu Sevugan, Mithilesh Mishra, Pananghat Gayathri, Mohan K Balasubramanian
Cytokinesis depends on a contractile actomyosin ring in many eukaryotes [1-3]. Myosin II is a key component of the actomyosin ring, although whether it functions as a motor or as an actin cross-linker to exert its essential role is disputed [1, 4, 5]. In Schizosaccharomyces pombe, the myo2-E1 mutation affects the upper 50 kDa sub-domain of the myosin II heavy chain, and cells carrying this lethal mutation are defective in actomyosin ring assembly at the non-permissive temperature [6, 7]. myo2-E1 also affects actomyosin ring contraction when rings isolated from permissive temperature-grown cells are incubated with ATP [8]...
February 21, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28238540/structural-adjustment-and-health-a-conceptual-framework-and-evidence-on-pathways
#19
Alexander E Kentikelenis
Economic reform programs designed by the International Monetary Fund and the World Bank-so-called 'structural adjustment programs'-have formed one of the most influential policy agendas of the past four decades. To gain access to financial support from these organizations, countries-often in economic crisis-have reduced public spending, limited the role of the state, and deregulated economic activity. This article identifies the multiple components of structural adjustment, and presents a conceptual framework linking them to health systems and outcomes...
February 23, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28238534/structural-insights-into-a-unique-dimeric-dead-box-helicase-csha-that-promotes-rna-decay
#20
Jennifer Huen, Chia-Liang Lin, Bagher Golzarroshan, Wan-Li Yi, Wei-Zen Yang, Hanna S Yuan
CshA is a dimeric DEAD-box helicase that cooperates with ribonucleases for mRNA turnover. The molecular mechanism for how a dimeric DEAD-box helicase aids in RNA decay remains unknown. Here, we report the crystal structure and small-angle X-ray scattering solution structure of the CshA from Geobacillus stearothermophilus. In contrast to typical monomeric DEAD-box helicases, CshA is exclusively a dimeric protein with the RecA-like domains of each protomer forming a V-shaped structure. We show that the C-terminal domains protruding outward from the tip of the V-shaped structure is critical for mediating strong RNA binding and is crucial for efficient RNA-dependent ATP hydrolysis...
February 18, 2017: Structure
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