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https://www.readbyqxmd.com/read/28092843/therapeutic-effects-of-bach1-sirna-on-human-breast-adenocarcinoma-cell-line
#1
Mansoor Aletaha, Behzad Mansoori, Ali Mohammadi, Mehdi Fazeli, Behzad Baradaran
BACKGROUND: Despite the great improvements in clinical and therapeutic techniques in recent years, many advanced breast cancer patients still died of the postoperative recurrence and metastasis of disease. Bach1 plays a role in the development of the invasive phenotypes of cancer, cell division and apoptosis in tumor cells. The aim of this study was to investigate the effect of specific bach1 siRNAs, on the proliferation, migration, invasive, induction of apoptosis, cell cycle arrest and alter EMT related miRNA of MDA-MB-468 cells (breast cancer)...
January 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28092755/coagulation-flocculation-sequential-with-fenton-or-photo-fenton-processes-as-an-alternative-for-the-industrial-textile-wastewater-treatment
#2
Edison GilPavas, Izabela Dobrosz-Gómez, Miguel Ángel Gómez-García
In this study, the industrial textile wastewater was treated using a chemical-based technique (coagulation-flocculation, C-F) sequential with an advanced oxidation process (AOP: Fenton or Photo-Fenton). During the C-F, Al2(SO4)3 was used as coagulant and its optimal dose was determined using the jar test. The following operational conditions of C-F, maximizing the organic matter removal, were determined: 700 mg/L of Al2(SO4)3 at pH = 9.96. Thus, the C-F allowed to remove 98% of turbidity, 48% of Chemical Oxygen Demand (COD), and let to increase in the BOD5/COD ratio from 0...
January 13, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28092380/chromatin-immunoprecipitation-in-microfluidic-droplets-towards-fast-and-cheap-analyses
#3
Bruno Teste, Jerome Champ, Arturo Londono-Vallejo, Stéphanie Descroix, Laurent Malaquin, Jean-Louis Viovy, Irena Draskovic, Guillaume Mottet
Genetic organization is governed by the interaction of DNA with histone proteins, and differential modifications of these proteins is a fundamental mechanism of gene regulation. Histone modifications are primarily studied through chromatin immunoprecipitation (ChIP) assays, however conventional ChIP procedures are time consuming, laborious and require a large number of cells. Here we report for the first time the development of ChIP in droplets based on a microfluidic platform combining nanoliter droplets, magnetic beads (MB) and magnetic tweezers (MT)...
January 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28092050/microwestern-arrays-for-systems-level-analysis-of-sh2-domain-containing-proteins
#4
Mark F Ciaccio, Richard B Jones
The Microwestern Array (MWA) method combines the scalability and miniaturization afforded by the Reverse Phase Lysate Array (RPLA) approach with the electrophoretic separation characteristic of the Western blot. This technology emulates the creation of an array of small Western blots on a single sheet of nitrocellulose allowing for the sensitive and quantitative measurement of hundreds of proteins from hundreds of cell lysates with minimal cost and maximal accuracy, precision, and reproducibility. The MWA is a versatile technology that can be easily configured for purposes such as antibody screening, cell signaling network inference, protein modification/phenotype regression analysis, and genomic/proteomic relationships...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28092045/sh2-domains-as-affinity-reagents-for-phosphotyrosine-protein-enrichment-and-proteomic-analysis
#5
Mi Ke, Bizhu Chu, Lin Lin, Ruijun Tian
Dynamic tyrosine phosphorylation is a key molecular modulation for many signal transduction events. Because of their low abundance and dynamic nature in cells, the detection and enrichment of phosphotyrosine proteins has long relied on specific antibodies, such as 4G10 and P-Tyr-100. Another well-established approach for phosphotyrosine proteins recognition and enrichment is by their specific binding domains, such as Src homology 2 (SH2) domains. In this chapter, we describe a typical analytical approach for purifying specific SH2 domains, enriching specific phosphotyrosine proteins from activated cells, mass spectrometry analysis, and related data analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28092036/creation-of-phosphotyrosine-superbinders-by-directed-evolution-of-an-sh2-domain
#6
Haiming Huang, Tomonori Kaneko, Sachdev S Sidhu, Shawn S C Li
Commercial antibodies raised against phosphotyrosine have been widely used as reagents to detect or isolate tyrosine-phosphorylated proteins from cellular samples. However, these antibodies are costly and are not amenable to in-house production in an academic lab setting. In this chapter, we describe a method to generate super-high affinity SH2 domains, dubbed the phosphotyrosine superbinders, by evolving a natural SH2 domain using the phage display technology. The superbinders are stable and can be easily produced in Escherichia coli in large quantities...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28091828/liver-x-receptor-%C3%AE-lxr%C3%AE-promoted-invasion-and-emt-of-gastric-cancer-cells-by-regulation-of-nf-%C3%AE%C2%BAb-activity
#7
Linhua Ji, Bin Zhang, Gang Zhao
Aberrant expression of Liver X receptor α (LXRα) has been frequently reported in various types of cancers excluding gastric cancer (GC). Moreover, the role of LXRα in human GC has not been previously reported. In this study, we investigated the effect of LXRα down-regulation on invasion and EMT of GC. The expression of LXRα in GC cell lines was detected by real-time PCR. The LXRα siRNA was transiently transfected into GC cells using Lipofectamine™ 2000 reagent. Subsequently, cell invasive ability was evaluated by Transwell assays...
January 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28091536/simple-efficient-and-controllable-synthesis-of-iodo-di-iodoarenes-via-ipsoiododecarboxylation-consecutive-iodination-strategy
#8
Yun Yang, Lijuan Zhang, Guo-Jun Deng, Hang Gong
A practical, efficient, and operationally simple strategy for the ipsoiododecarboxylation and di-iodination of aromatic carboxylic acids using the low-cost commercial reagent succinimide (NIS) as iodine source is reported. This iodination or di-iodination process can be easily controlled through reaction conditions, thereby providing corresponding iodination or di-iodination products with high yields. Furthermore, these two reactions can be easily scaled up to gram-scale by using palladium catalyst (0.66 mol%), which provides high isolated yield...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28090707/organoselenium-catalyzed-regioselective-c-h-pyridination-of-1-3-dienes-and-alkenes
#9
Lihao Liao, Ruizhi Guo, Xiaodan Zhao
An efficient approach for organoselenium-catalyzed regioselective C-H pyridination of 1,3-dienes to form pyridinium salts has been developed. This method was also successfully applied to direct C-H pyridination of alkenes. Fluoropyridinium reagents, or initially loaded pyridine derivatives, acted as pyridine sources in the pyridination reactions. The obtained pyridinium salts could be further converted under different conditions. This work is the first example of catalytic C-2 direct C-H functionalization of 1,3-dienes and the first case of organoselenium-catalyzed C-H pyridination...
January 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28090124/practical-synthetic-procedures-for-the-iron-catalyzed-intermolecular-olefin-aminohydroxylation-using-functionalized-hydroxylamines
#10
Cheng-Liang Zhu, Deng-Fu Lu, Jeffrey D Sears, Zhen-Xin Jia, Hao Xu
A set of practical synthetic procedures for the iron-catalyzed intermolecular olefin aminohydroxylation reactions in gram scale is reported. In these transformations, a bench-stable functionalized hydroxylamine is applied as the amination reagent. This method is compatible with a broad range of synthetically valuable olefins including those that are incompatible with the existing aminohydroxylation methods. It also provides valuable amino alcohol building blocks with regio- and stereo-chemical arrays that are complementary to known methods...
September 2016: Synthesis
https://www.readbyqxmd.com/read/28089735/effects-of-simvastatin-on-cat-1-mediated-arginine-transport-and-no-level-under-high-glucose-conditions-in-conditionally-immortalized-rat-inner-blood-retinal-barrier-cell-lines-tr-ibrb
#11
Temdara Tun, Young-Sook Kang
OBJECTIVE: Hyperglycemia causes the breakdown of the blood-retinal barrier by impairing endothelial nitric oxide synthase (eNOS) function. Statins have many pleiotropic effects such as improving endothelial barrier permeability and increasing eNOS mRNA stability. The objective of this study was to determine effect of simvastatin on l-arginine transport and NO production under high-glucose conditions in conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB). METHODS: Changes in l-arginine transport uptake and, expression levels of cationic amino acid transporter 1 (CAT-1) and eNOS mRNA were investigated after pre-treatment with simvastatin and NOS inhibitors (l-NMMA and l-NAME) under high-glucose conditions using TR-iBRB, an in vitro model of iBRB...
January 12, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28089067/effect-of-mobile-phase-additives-on-solute-retention-at-low-aqueous-ph-in-hydrophilic-interaction-liquid-chromatography
#12
David V McCalley
Trifluoracetic acid (TFA) added to the aqueous acetonitrile mobile phase induces some unexpected changes in the ionic component of retention in hydrophilic interaction separations when using Type B silica and amide-bonded silica columns. TFA use results in anion exchange properties which contrast with the cation exchange typically found with ammonium salt buffers. The significant cation exchange properties of silica hydride columns are also moderated by TFA. Similar behaviour was shown in a metal- free amide column operated on a system washed with a metal complexing agent, suggesting that adsorbed metal cations were not responsible for this anion exchange behaviour...
December 15, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28088504/novel-insights-into-the-transport-mechanism-of-the-human-amino-acid-transporter-lat1-slc7a5-probing-critical-residues-for-substrate-translocation
#13
Lara Napolitano, Michele Galluccio, Mariafrancesca Scalise, Chiara Parravicini, Luca Palazzolo, Ivano Eberini, Cesare Indiveri
BACKGROUND: LAT1 (SLC7A5) is the transport competent unit of the heterodimer formed with the glycoprotein CD98 (SLC3A2). It catalyzes antiport of His and some neutral amino acids such as Ile, Leu, Val, Cys, Met, Gln and Phe thus being involved in amino acid metabolism. Interestingly, LAT1 is over-expressed in many human cancers that are characterized by increased demand of amino acids. Therefore LAT1 was recently acknowledged as a novel target for cancer therapy. However, knowledge on molecular mechanism of LAT1 transport is still scarce...
January 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28088220/anti-inflammatory-potential-of-ellagic-acid-gallic-acid-and-punicalagin-a-b-isolated-from-punica-granatum
#14
Lamees A BenSaad, Kah Hwi Kim, Chin Chew Quah, Wee Ric Kim, Mustafa Shahimi
BACKGROUND: Punica granatum (pomegranate), an edible fruit originating in the Middle East, has been used as a traditional medicine for treatment of pain and inflammatory conditions such as peptic ulcer. The numerous risks associated with nonsteroidal anti-inflammatory drugs (NSAIDs) for treatment of pain and inflammation give rise to using medicinal herbs as alternative therapies. This study aimed to evaluate the anti-inflammatory effect of isolated compounds from the ethyl acetate (EtOAc) fraction of P...
January 14, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28086859/cheongsangbangpung-tang-ameliorated-the-acute-inflammatory-response-via-the-inhibition-of-nf-%C3%AE%C2%BAb-activation-and-mapk-phosphorylation
#15
Seon Young Kim, Sang Mi Park, Min Hwangbo, Jong Rok Lee, Sung Hui Byun, Sae Kwang Ku, Il Je Cho, Sang Chan Kim, Seon Young Jee, Sook Jahr Park
BACKGROUND: Cheongsangbangpung-tang (CBT) is a traditional herbal formula used in Eastern Asia to treat heat-related diseases and swellings in the skin. The present study was conducted to evaluate the anti-inflammatory effects of cheongsangbangpung-tang extract (CBTE) both in vitro and in vivo. METHODS: The in vitro effects of CBTE on the lipopolysaccharide (LPS)-induced production of inflammation-related proteins were examined in RAW 264.7 cells. The levels of nitric oxide (NO) were measured with the Griess reagent...
January 13, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28081362/lithium-carbonate-recovery-from-cathode-scrap-of-spent-lithium-ion-battery-a-closed-loop-process
#16
Wenfang Gao, Xihua Zhang, Xiaohong Zheng, Xiao Lin, Hongbin Cao, Yi Zhang, Zhi H I Sun
A closed-loop process to recover lithium carbonate from cathode scrap of lithium-ion battery (LIB) is developed. Lithium could be selectively leached into solution using formic acid while aluminum remained as metallic form and most of other metals from the cathode scrap could be precipitated out. This phenomenon clearly demonstrates that formic acid can be used for lithium recovery from cathode scrap, as both leaching and separation reagent. By investigating the effects of different parameters including temperature, formic acid concentration, H2O2 amount and solid to liquid ratio, the leaching rate of Li can reach 99...
January 12, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28081286/carbon-carbon-bond-formation-in-a-weak-ligand-field-leveraging-open-shell-first-row-transition-metal-catalysts
#17
Paul James Chirik
Unique features of Earth abundant transition metal catalysts are reviewed in the context of catalytic carbon-carbon bond forming reactions. Aryl-substituted bis(imino)pyridine iron and cobalt dihalide compounds, when activated with alkyl aluminum reagents, form highly active catalysts for the polymerization of ethylene. Open shell iron and cobalt alkyl complexes have been synthesized that serve as single component olefin polymerization catalysts. Reduced bis(imino)pyridine iron- and cobalt dinitrogen compounds have also been discovered that promote the unique [2+2] cycloaddition of unactivated terminal alkenes...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28081203/mechanism-and-parameter-optimization-of-fenton-s-reagent-integrated-with-surfactant-pretreatment-to-improve-sludge-dewaterability
#18
Yi Xing, Zhiqiang Wang, Chen Hong, Qiang Yang, Lihui Feng, Mengmeng Jia, Yifei Li
Sludge dewatering can effectively reduce the volume and mass of sludge for subsequent treatment and disposal. The work validated the potential of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) in improving sludge dewaterability and proposed the mechanism of joint conditioning. The composite conditioner dosage was optimized using response surface methodology. Results indicated the good conditioning capability of the composite conditioners. The optimum dosages for H2O2, Fe2+, and DDBAC were 44...
2017: PloS One
https://www.readbyqxmd.com/read/28080908/antidog-igg-secondary-antibody-successfully-detects-igg-in-a-variety-of-aquatic-mammals
#19
Katherine Roehl, Mark Jankowski, Erik Hofmeister
Serological tests play an important role in the detection of wildlife diseases. However, while there are many commercial assays and reagents available for domestic species, there is a need to develop efficient serological assays for wildlife. In recent years, marine mammals have represented a wildlife group with emerging infectious diseases, such as influenza, brucellosis, and leptospirosis. However, with the exception of disease-agent-specific assays or functional assays, few reports describe the use of antibody detection assays in marine mammals...
December 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28080905/plasma-protein-electrophoresis-and-select-acute-phase-proteins-in-healthy-bonnethead-sharks-sphyrna-tiburo-under-managed-care
#20
Michael W Hyatt, Cara L Field, Tonya M Clauss, Kristopher L Arheart, Carolyn Cray
Preventative health care of elasmobranchs is an important but understudied field of aquatic veterinary medicine. Evaluation of inflammation through the acute phase response is a valuable tool in health assessments. To better assess the health of bonnethead sharks ( Sphyrna tiburo ) under managed care, normal reference intervals of protein electrophoresis (EPH) and the acute phase proteins, C-reactive protein (CRP) and haptoglobin (HP), were established. Blood was collected from wild caught, captive raised bonnethead sharks housed at public aquaria...
December 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
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