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https://www.readbyqxmd.com/read/28330158/a-simple-and-efficient-agrobacterium-tumefaciens-mediated-plant-transformation-of-brassica-rapa-ssp-pekinensis
#1
Venkidasamy Baskar, Baniekal H Gangadhar, Se Won Park, Shivraj Hariram Nile
The present study aims to investigate the numerous factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Chinese cabbage (Brassica rapa ssp. pekinensis). Factors affecting transformation efficiency, such as age of explants, Agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of Agrobacterium were examined. The pre-cultured hypocotyls from young seedlings prior to exposure to Agrobacterium showed higher shoot regeneration. The plant transformation with the modest A...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28300944/phytophagy-of-the-predator-podisus-nigrispinus-dallas-1851-hemiptera-pentatomidae-fed-on-prey-and-brassicaceae
#2
J F J Grigolli, M M Kubota Grigolli, D G Ramalho, A L Martins, A M Vacari, S A De Bortoli
The purpose of this study was to investigate the development and reproduction of the zoophytophagous predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) fed kale, broccoli and cabbage affects its. Nymphs and adults of this predator were fed on larvae of Plutella xylostella (L.) (Lepidoptera: Plutellidae) as prey with kale, cabbage, or broccoli. In the nymph period, the duration and prey consumption were similar with all the Brassicacea cultivar. However, nymph viability was higher for predators with broccoli leaves...
March 13, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28290614/establishment-and-application-of-molecular-id-in-the-main-inbred-lines-of-chinese-cabbage
#3
X X Zhang, X B Tang, Y Liu, Y W Zhang
Chinese cabbage is an important cruciferous vegetable in China. The differences in the morphology and other characteristics of the different varieties of Chinese cabbage are generally caused by their different genes. Using the simple sequence repeat (SSR) DNA molecular markers is an effective way to identify different genotypes. The identification of a genetic relationship is a key point in the breeding process, and it plays an important role in guiding parent selection and breeding of high-yield varieties...
March 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28283189/metal-ions-and-phosphatidylinositol-4-5-bisphosphate-as-interacting-effectors-of-%C3%AE-type-plant-phospholipase-d
#4
Lars Dreßler, Florian Michel, Iris Thondorf, Johanna Mansfeld, Ralph Golbik, Renate Ulbrich-Hofmann
Plant phospholipases D (PLD) are typically characterized by a C2 domain with at least two Ca(2+) binding sites. In vitro, the predominantly expressed α-type PLDs need 20-100 mM CaCl2 for optimum activity, whereas the essential activator of β- or γ-type PLDs, phosphatidylinositol 4,5-bisphosphate (PIP2), plays a secondary role. In the present paper, we have studied the interplay between PIP2 and metal ion activation of the well-known α-type PLD from cabbage (PLDα). With mixed micelles containing phosphatidyl-p-nitrophenol as substrate, PIP2-concentrations in the nanomolar range are able to activate the enzyme in addition to the essential Ca(2+) activation...
March 7, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28280510/determination-of-levels-of-organochlorine-organophosphorus-and-pyrethroid-pesticide-residues-in-vegetables-from-markets-in-dar-es-salaam-by-gc-ms
#5
John A M Mahugija, Farhat A Khamis, Esther H J Lugwisha
This study investigated the levels of pesticides and metabolites in vegetables from major markets in Dar es Salaam city, Tanzania. Samples of fresh cabbage, spinach, and onions from the markets were analysed for pesticide residues. Extraction was performed using acetone followed by dichloromethane : cyclohexane mixture and the extracts were cleaned up using Florisil. The compounds were determined by gas chromatography-mass spectrometry (GC-MS). Pesticides and metabolites were detected in 72.2% of the samples...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28278628/phytotoxic-antibacterial-and-antioxidant-activities-of-mycotoxins-and-other-metabolites-from-trichoderma-sp
#6
Ju-Cheng Zhang, Guang-Yi Chen, Xiao-Zhan Li, Ming Hu, Bang-Yan Wang, Bao-Hui Ruan, Hao Zhou, Li-Xing Zhao, Jun Zhou, Zhong-Tao Ding, Ya-Bin Yang
A new natural mycotoxin was isolated from the fermentation broth of Trichoderma sp. Jing-8 and the structure was determined as alternariol 1'-hydroxy-9-methyl ether (1), together with twelve known compounds. The structures were elucidated on the basis of their 1D, 2D NMR spectra and mass spectrometric data. Compounds 1, 8 and 9 indicated inhibitions against germination of the seeds of cabbage with MICs < 3 μg/mL. The compound 1 showed the antibacterial activity against Bacillus subtilis and Staphylococcus aureus with MICs at 64 μg/mL...
March 2, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28276455/corrigendum-the-green-peach-aphid-myzus-persicae-perform-better-on-pre-infested-chinese-cabbage-brassica-pekinensis-by-enhancing-host-plant-nutritional-quality
#7
He-He Cao, Hui-Ru Liu, Zhan-Feng Zhang, Tong-Xian Liu
No abstract text is available yet for this article.
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28276138/analysis-of-oilseed-rape-stem-weevils-chemical-control-using-damage-rating-scale
#8
Željko Milovac, Miroslav Zorić, Filip Franeta, Sreten Terzić, Olivera Petrović Obradović, Ana Marjanović Jeromela
BACKGROUND: Rape stem weevil (Ceutorhynchus napi Gyll.) and cabbage stem weevil (C. pallidactylus Marsh.) can cause significant yield losses to oilseed rape (Brassica napus L.), and chemical control is often needed to protect crops from these pests. The efficacy of six insecticides, chlorpyriphos + cypermethrin, bifenthrin, alpha-cypermethrin, pirimiphos-methyl, thiacloprid and tau-fluvalinate, was tested in a four year field trial. Besides the standard efficacy analysis expressed through the number of larvae per stem, a damage rating scale was introduced and modelled using a regression model for ordinal categorical data...
March 8, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28273400/fitness-of-bt-resistant-cabbage-loopers-on-bt-cotton-plants
#9
Guillaume Tetreau, Ran Wang, Ping Wang
Development of resistance to the insecticidal toxins from Bacillus thuringiensis (Bt) in insects is the major threat to the continued success of transgenic Bt-crops in agriculture. The fitness of Bt-resistant insects on Bt- and non-Bt-plants is a key parameter that determines the development of Bt-resistance in insect populations. In this study, a comprehensive analysis of the fitness of Bt-resistant Trichoplusia ni strains on Bt-cotton leaves was conducted. The Bt-resistant T. ni strains carried two genetically independent mechanisms of resistance to Bt toxins Cry1Ac and Cry2Ab...
March 8, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28265282/fine-mapping-and-analysis-of-cgl1-a-gene-conferring-glossy-trait-in-cabbage-brassica-oleracea-l-var-capitata
#10
Zezhou Liu, Zhiyuan Fang, Mu Zhuang, Yangyong Zhang, Honghao Lv, Yumei Liu, Zhansheng Li, Peitian Sun, Jun Tang, Dongming Liu, Zhenxian Zhang, Limei Yang
Cuticular waxes covering the outer plant surface impart a whitish appearance. Wax-less cabbage mutant shows glossy in leaf surface and plays important roles in riching cabbage germplasm resources and breeding brilliant green cabbage. This is the first report describing the characterization and fine-mapping of a wax biosynthesis gene using a novel glossy Brassica oleracea mutant. In the present paper, we identified a glossy cabbage mutant (line10Q-961) with a brilliant green phenotype. Genetic analyses indicated that the glossy trait was controlled by a single recessive gene...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28262388/enzymatic-comparison-and-mortality-of-beauveria-bassiana-against-cabbage-caterpillar-pieris-brassicae-linn
#11
Manish Dhawan, Neelam Joshi
Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (10(9)conidia/ml), and the minimum mortality (30...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28247857/adherence-to-a-healthy-nordic-food-index-and-risk-of-myocardial-infarction-in-middle-aged-danes-the-diet-cancer-and-health-cohort-study
#12
V B Gunge, I Andersen, C Kyrø, C P Hansen, C C Dahm, J Christensen, A Tjønneland, A Olsen
BACKGROUND/OBJECTIVES: For decades, the Mediterranean diet has been in focus regarding healthy eating as it has been associated with reduced risk of non-communicable diseases. Less interest has been given to health benefits of other regional diets. The aim of the present study was to assess whether adherence to a healthy Nordic food index was associated with lower risk of myocardial infarction (MI) among middle-aged Danes. SUBJECTS/METHODS: Data were obtained from the Danish Diet, Cancer and Health cohort study of 57 053 men and women aged 50-64 years recruited between 1993 and 1997...
March 1, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28239387/comprehensive-analysis-of-the-cdpk-snrk-superfamily-genes-in-chinese-cabbage-and-its-evolutionary-implications-in-plants
#13
Peng Wu, Wenli Wang, Weike Duan, Ying Li, Xilin Hou
The CDPK-SnRK (calcium-dependent protein kinase/Snf1-related protein kinase) gene superfamily plays important roles in signaling pathways for disease resistance and various stress responses, as indicated by emerging evidence. In this study, we constructed comparative analyses of gene structure, retention, expansion, whole-genome duplication (WGD) and expression patterns of CDPK-SnRK genes in Brassica rapa and their evolution in plants. A total of 49 BrCPKs, 14 BrCRKs, 3 BrPPCKs, 5 BrPEPRKs, and 56 BrSnRKs were identified in B...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28231170/fermentation-assisted-extraction-of-isothiocyanates-from-brassica-vegetable-using-box-behnken-experimental-design
#14
Amit K Jaiswal, Nissreen Abu-Ghannam
Recent studies showed that Brassica vegetables are rich in numerous health-promoting compounds such as carotenoids, polyphenols, flavonoids, and glucosinolates (GLS), as well as isothiocyanates (ITCs) and are involved in health promotion upon consumption. ITCs are breakdown products of GLS, and typically used in the food industry as a food preservative and colouring agent. They are also used in the pharmaceutical industry due to their several pharmacological properties such as antibacterial, antifungal, antiprotozoal, anti-inflammatory, and chemoprotective effects, etc...
November 4, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28221925/genetic-diversity-and-antibiotic-resistance-of-escherichia-coli-isolates-from-different-leafy-green-production-systems
#15
Mosimanegape Jongman, Lise Korsten
Foodborne disease outbreaks linked to contaminated irrigation water and fresh produce are a public health concern. The presence of Escherichia coli isolates from irrigation water and leafy green vegetables in different food production systems (large commercial farms, small-scale farms, and homestead gardens) was investigated. The prevalence of antibiotic resistance and virulence in these isolates was further assessed, and links between water source and irrigated crops were identified using antimicrobial and genotypic analyses...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221891/microbial-quality-safety-and-pathogen-detection-by-using-quantitative-pcr-of-raw-salad-vegetables-sold-in-dhanbad-city-india
#16
Sujeet K Mritunjay, Vipin Kumar
Consumption of ready-to-eat fresh vegetables has increased worldwide, with a consequent increase in outbreaks caused by foodborne pathogens. In the Indian subcontinent, raw fresh vegetables are usually consumed without washing or other decontamination procedures, thereby leading to new food safety threats. In this study, the microbiological quality and pathogenic profile of raw salad vegetables was evaluated through standard protocols. In total, 480 samples (60 each of eight different salad vegetables) of cucumber, tomato, carrot, coriander, cabbage, beetroot, radish, and spinach were collected from different locations in Dhanbad, a city famous for its coal fields and often called the "Coal Capital of India...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28197590/rats-urinary-metabolomes-reveal-the-potential-roles-of-functional-foods-and-exercise-in-obesity-management
#17
Mohamed A Farag, N M Ammar, T E Kholeif, N S Metwally, N M El-Sheikh, Ludger A Wessjohann, A Z Abdel-Hamid
The complexity of the metabolic changes in obese individuals still presents a challenge for the understanding of obesity-related metabolic disruptions and for obesity management. In this study, a gas chromatography mass spectrometry (GC-MS) based metabolomics approach targeting urine metabolism has been applied to assess the potential roles of functional foods and exercise for obesity management in rats. Male albino rats diagnosed as obese via histopathology and biochemical assays were administered functional foods in common use for obesity management including pomegranate, grapefruit, and red cabbage juice extracts in parallel with swimming exercise...
February 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28181580/penetration-enhancement-underlies-synergy-of-plant-essential-oil-terpenoids-as-insecticides-in-the-cabbage-looper-trichoplusia-ni
#18
Jun-Hyung Tak, Murray B Isman
Many plant essential oils and their terpenoid constituents possess bioactivities including insecticidal activity, and they sometimes act synergistically when mixed. Although several hypotheses for this have been proposed, the underlying mechanism has not been fully elucidated thus far. In the present study, we report that in larvae of the cabbage looper, Trichoplusia ni, most synergistic or antagonistic insecticidal activities among mixtures of plant essential oil constituents are pharmacokinetic effects, owing to changes in solubility as well as spreadability on a wax layer...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176652/mechanisms-for-inhibition-of-colon-cancer-cells-by-sulforaphane-through-epigenetic-modulation-of-microrna-21-and-human-telomerase-reverse-transcriptase-htert-down-regulation
#19
Samantha L Martin, Rishabh Kala, Trygve O Tollefsbol
Epigenetic modulations such as histone modifications are becoming increasingly valued for their ability to modify genes without altering the DNA sequence. Many bioactive compounds have been shown to alter genetic and epigenetic profiles in various forms of 6 cancers. Of the many dietary phytochemicals, sulforaphane (SFN), found in cruciferous vegetables such as kale, cabbage and broccoli sprouts, has been present as one of the most potent (histone deacetylase) HDAC inhibitors to date. Recently, it has been 9 identified that HDAC inhibitors may play a vital role in regulating microRNAs (miRNAs) in many human cancers...
February 5, 2017: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/28169368/genome-wide-analysis-of-auxin-transport-genes-identifies-the-hormone-responsive-patterns-associated-with-leafy-head-formation-in-chinese-cabbage
#20
Li-Wei Gao, Shan-Wu Lyu, Jun Tang, Dao-Yun Zhou, Guusje Bonnema, Dong Xiao, Xi-Lin Hou, Chang-Wei Zhang
Auxin resistant 1/like aux1 (AUX/LAX), pin-formed (PIN) and ATP binding cassette subfamily B (ABCB/MDR/PGP) are three families of auxin transport genes. The development-related functions of the influx and efflux carriers have been well studied and characterized in model plants. However, there is scant information regarding the functions of auxin genes in Chinese cabbage and the responses of exogenous polar auxin transport inhibitors (PATIs). We conducted a whole-genome annotation and a bioinformatics analysis of BrAUX/LAX, BrPIN, and BrPGP genes in Chinese cabbage...
February 7, 2017: Scientific Reports
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