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https://www.readbyqxmd.com/read/28918746/escherichia-coli-signal-peptidase-recognizes-and-cleaves-archaeal-signal-sequence
#1
Majida Atta Muhammad, Samia Falak, Naeem Rashid, Qurra-Tul-Ann Afza Gardner, Nasir Ahmad, Tadayuki Imanaka, Muhammad Akhtar
Tk1884, an open reading frame encoding α-amylase in Thermococcus kodakarensis, was cloned with the native signal sequence and expressed in Escherichia coli. Heterologous gene expression resulted in secretion of the recombinant protein to the extracellular culture medium. Extracellular α-amylase activity gradually increased after induction. Tk1884 was purified from the extracellular medium, and its molecular mass determined by electrospray ionization mass spectrometry indicated the cleavage of a few amino acids...
July 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28913664/supplementation-of-enzyme-treated-soy-protein-saves-dietary-protein-and-promotes-digestive-and-absorptive-ability-referring-to-tor-signaling-in-juvenile-fish
#2
Weiwei Xiao, Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang, Yongan Zhang, Xiaoqiu Zhou
This study was conducted to evaluate the effect of enzyme-treated soy protein (ETSP) supplementation in the low-protein diet on growth performance, digestive and absorptive capacities, and related signaling molecules' gene expressions in juvenile Jian carp. The results showed that percent weight gain (PWG), specific growth rate (SGR), and feed intake (FI) were decreased by reducing dietary protein from 34 to 32% (P < 0.05). Supplying low-protein diet with optimal ETSP increased previously mentioned indices of juvenile Jian carp (P < 0...
September 14, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28887188/an-extremely-thermostable-maltogenic-amylase-from-staphylothermus-marinus-bacillus-expression-of-the-gene-and-its-application-in-genistin-glycosylation
#3
Xiaolei Li, Yujuan Wang, Jong-Tae Park, Liwei Gu, Dan Li
The most extremely thermostable maltogenic amylase (SMMA) from archaeon Staphylothermus marinus has many potential applications in food processing. To ensure safety of microbial origin, a recombinant plasmid containing the enzymic gene and a constitutive promoter AmyR2 was constructed, and then transformed into a GRAS microorganism Bacillus subtilis. The purified SMMA from the liquid cultures of Bacillus has a specific activity of 66.96U/mg, two times more than that from Escherichia coli. SMMA was further employed to catalyze the genistion glycosylation using γ-CD as both glucosyl donors and solubilizer...
September 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28878591/mutations-in-the-gene-of-the-g%C3%AE-subunit-of-the-heterotrimeric-g-protein-are-the-cause-for-the-brachytic1-semi-dwarf-phenotype-in-barley-and-applicable-for-practical-breeding
#4
Ilka Braumann, Christoph Dockter, Sebastian Beier, Axel Himmelbach, Finn Lok, Udda Lundqvist, Birgitte Skadhauge, Nils Stein, Shakhira Zakhrabekova, Ruonan Zhou, Mats Hansson
BACKGROUND: Short-culm mutants have been widely used in breeding programs to increase lodging resistance. In barley (Hordeum vulgare L.), several hundreds of short-culm mutants have been isolated over the years. The objective of the present study was to identify the Brachytic1 (Brh1) semi-dwarfing gene and to test its effect on yield and malting quality. RESULTS: Double-haploid lines generated through a cross between a brh1.a mutant and the European elite malting cultivar Quench, showed good malting quality but a decrease in yield...
2018: Hereditas
https://www.readbyqxmd.com/read/28873206/characterization-and-phylogenetic-affiliation-of-actinobacteria-from-tropical-soils-with-potential-uses-for-agro-industrial-processes
#5
J C M Dornelas, J E F Figueiredo, C S de Abreu, U G P Lana, C A Oliveira, I E Marriel
Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology...
August 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28846395/comparative-transcriptomic-analysis-in-the-paddy-rice-under-storage-and-identification-of-differentially-regulated-genes-in-response-to-high-temperature-and-humidity
#6
Chanjuan Zhao, Junqi Xie, Li Li, Chongjiang Cao
The transcriptomes of paddy rice in response to high temperature and humidity were studied using high-throughput RNA sequencing approach. Effects of high temperature and humidity on the sucrose and starch content, α/β-amylase activity were also investigated. Results showed that 6876 differentially-expressed genes (DEGs) were identified in paddy rice under high temperature and humidity storage. Importantly, 12 DEGs that were down-regulated were fell into 'starch and sucrose pathway'. The quantitative real-time PCR (qRT-PCR) assays indicated that expression of these 12 DEGs was significantly decreased, which was in parallel with the reduced level of enzyme activities and the content of sucrose and starch in paddy rice stored at high temperature and humidity conditions compared with the control group...
August 28, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28838812/polyphasic-characterization-of-bacteria-obtained-from-upland-rice-cultivated-in-cerrado-soil
#7
Lívia Fabiana Braga, Fênix Araújo de Oliveira, Eva Aparecida Prado do Couto, Karina Freire d'Eça Nogueira Santos, Enderson Petrônio de Brito Ferreira, Claudia Cristina Garcia Martin-Didonet
This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase...
August 5, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28836148/anti-diabetic-actions-of-esculentin-2cha-1-30-and-its-stable-analogues-in-a-diet-induced-model-of-obesity-diabetes
#8
Srividya Vasu, Opeolu O Ojo, R Charlotte Moffett, J Michael Conlon, Peter R Flatt, Yasser H A Abdel-Wahab
Actions of esculentin-2CHa(1-30) (GFSSIFRGVAKFASKGLGKDLAKLGVDLVA) and its analogues, ([D-Arg(7), D-Lys(15), D-Lys(23)]-esculentin-2CHa(1-30) and [Lys(15)-octanoate]-esculentin-2CHa(1-30), were evaluated in high-fat fed NIH Swiss mice with impaired glucose tolerance and insulin resistance. Twice-daily i.p. administration of the esculentin-2CHa(1-30) peptides (75 nmol/kg body weight) or exendin-4 (25 nmol/kg) for 28 days reduced body weight, without altering cumulative energy intake. All peptides reduced blood glucose levels by 6-12 mmol/l concomitant with lower plasma insulin levels, with significance evident from day 6...
August 23, 2017: Amino Acids
https://www.readbyqxmd.com/read/28822907/leaf-carbohydrates-influence-transcriptional-and-post-transcriptional-regulation-of-nocturnal-carboxylation-and-starch-degradation-in-the-facultative-cam-plant-mesembryanthemum-crystallinum
#9
Tahar Taybi, John C Cushman, Anne M Borland
Nocturnal degradation of transitory starch is a limiting factor for the optimal function of crassulacean acid metabolism and must be coordinated with phosphoenolypyruvate carboxylase (PEPC)-mediated CO2 uptake to optimise carbon gain over the diel cycle. The aim of this study was to test the hypothesis that nocturnal carboxylation is coordinated with starch degradation in CAM via a mechanism whereby the products of these pathways regulate diel transcript abundance and enzyme activities for both processes. To test this hypothesis, a starch and CAM-deficient mutant of Mesembryanthemum crystallinum was compared with wild type plants under well-watered and saline (CAM-inducing) conditions...
August 5, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28819794/in-vitro-selection-and-identification-of-potential-probiotics-isolated-from-the-gastrointestinal-tract-of-nile-tilapia-oreochromis-niloticus
#10
Rasha M Reda, Khaled M Selim, Hassanin M El-Sayed, M A El-Hady
Fish gut bacteria can be used as probiotics for aquaculture. The aim of this study is to screen and identify beneficial probiotic bacteria from the gut of Nile tilapia, Oreochromis niloticus. Nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, Aeromonas sobria, Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas putida, and Staphylococcus aureus. Further tests showed that such isolates can survive in the presence of high bile concentration (10%) and at different acidic pH values...
August 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28819233/biology-physiology-and-gene-expression-of-grasshopper-oedaleus-asiaticus-exposed-to-diet-stress-from-plant-secondary-compounds
#11
Xunbing Huang, Jingchuan Ma, Xinghu Qin, Xiongbing Tu, Guangchun Cao, Guangjun Wang, Xiangqun Nong, Zehua Zhang
We studied the role of plant primary and secondary metabolites in mediating plant-insect interactions by conducting a no-choice single-plant species field experiment to compare the suitability, enzyme activities, and gene expression of Oedaleus asiaticus grasshoppers feeding on four host and non-host plants with different chemical traits. O. asiaticus growth showed a positive relationship to food nutrition content and a negative relationship to secondary compounds content. Grasshopper amylase, chymotrypsin, and lipase activities were positively related to food starch, crude protein, and lipid content, respectively...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811584/nanopriming-technology-for-enhancing-germination-and-starch-metabolism-of-aged-rice-seeds-using-phytosynthesized-silver-nanoparticles
#12
Wuttipong Mahakham, Ajit K Sarmah, Santi Maensiri, Piyada Theerakulpisut
Application of nanomaterials for agriculture is relatively new as compared to their use in biomedical and industrial sectors. In order to promote sustainable nanoagriculture, biocompatible silver nanoparticles (AgNPs) have been synthesized through green route using kaffir lime leaf extract for use as nanopriming agent for enhancing seed germination of rice aged seeds. Results of various characterization techniques showed the successful formation of AgNPs which were capped with phytochemicals present in the plant extract...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811162/engineering-peptidoglycan-degradation-related-genes-of-bacillus-subtilis-for-better-fermentation-processes
#13
Liang Zhao, Jianwen Ye, Jing Fu, Guo-Qiang Chen
In this study, Bacillus subtilis 168 Δupp was engineered to change the bacterial shapes. Namely, some peptidoglycan hydrolase related genes were inactivated individually or in different combinations, including sigD, lytE, lytF, lytC, lytD and lytG. Inactivations of these genes resulted in various intensities of blockages on cell division, leading to elongation of bacterial cells. The resulted fiber phenotypes showed different lengths ranging from tens of microns to several millimeters. Mutants with multiple gene inactivations such as ΔsigDΔlytEΔlytD showed more easily precipitated phenomenon, obviously increased growth rate, more sensitive to antibiotics and improved α-amylase production compared with that of B...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28804584/the-expression-of-activating-receptor-gene-of-natural-killer-cells-klrc3-in-patients-with-%C3%A2-type-1-diabetes-mellitus-t1dm
#14
Dalia Shalaby, Marwa Saied, Doaa Khater, Abla Abou Zeid
OBJECTIVES: To identify the possible role of natural killer (NK) cells in the pathogenesis of type 1 diabetes mellitus (T1DM) through studying the expression of the KLRC3 gene, which encodes the NK cell activating receptor (NKG2E). METHODS: This study was conducted at Alexandria University Children's Hospital from April to October 2015. The study was conducted with 30 newly diagnosed T1DM patients (15 males and 15 females), aged 7-13 years (10.6±1.8 years) and 20 non-diabetic subjects served as age- and sex-matched controls...
July 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/28783453/development-of-human-salivary-gland-like-tissue-in-vitro
#15
Marc Burghartz, Simon Lennartz, Matthias Schweinlin, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg, Gudrun Steußloff, Agmal Scherzad, Kathrin Radeloff, Christian Ginzkey, Heike Walles, Marco Metzger
The loss of salivary gland function caused by radiation therapy of the head and neck is a serious condition and it affects a patient's quality of life. The current lack of effective therapies demands new options to be explored. This study tested whether human salivary gland epithelial cells (SGECs) could be successfully cultured on a decellularized porcine gut matrix (SIS-muc) in both mono- and coculture with microvascular endothelial cells (mvECs). By performing immunofluorescence imaging, transmission as well as scanning electron microscopy (SEM), quantitative polymerase chain reaction (qPCR), and an amylase enzyme assay, it was investigated as to what extent the three-dimensional (3D)-cultured cells could maintain their molecular differentiation and the production of working α-amylase (α-AMY) compared with two-dimensional (2D) culture...
August 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28729759/transcriptome-wide-identification-and-characterization-of-starch-branching-enzyme-in-finger-millet
#16
Rajhans Tyagi, Apoorv Tiwari, Vijay Kumar Garg, Sanjay Gupta
Starch-branching enzymes (SBEs) are one of the four major enzyme classes involved in starch biosynthesis in plants and play an important role in determining the structure and physical properties of starch granules. Multiple SBEs are involved in starch biosynthesis in plants. Finger millet is calcium rich important serial crop belongs to grass family and the transcriptome data of developing spikes is available on NCBI. In this study it was try to find out the gene sequence of starch branching enzyme and annotate the sequence and submit the sequence for further use...
2017: Bioinformation
https://www.readbyqxmd.com/read/28727805/turgor-responsive-starch-phosphorylation-in-oryza-sativa-stems-a-primary-event-of-starch-degradation-associated-with-grain-filling-ability
#17
Hiroshi Wada, Chisato Masumoto-Kubo, Koichi Tsutsumi, Hiroshi Nonami, Fukuyo Tanaka, Haruka Okada, Rosa Erra-Balsells, Kenzo Hiraoka, Taiken Nakashima, Makoto Hakata, Satoshi Morita
Grain filling ability is mainly affected by the translocation of carbohydrates generated from temporarily stored stem starch in most field crops including rice (Oryza sativa L.). The partitioning of non-structural stem carbohydrates has been recognized as an important trait for raising the yield ceiling, yet we still do not fully understand how carbohydrate partitioning occurs in the stems. In this study, two rice subspecies that exhibit different patterns of non-structural stem carbohydrates partitioning, a japonica-dominant cultivar, Momiroman, and an indica-dominant cultivar, Hokuriku 193, were used as the model system to study the relationship between turgor pressure and metabolic regulation of non-structural stem carbohydrates, by combining the water status measurement with gene expression analysis and a dynamic prefixed 13C tracer analysis using a mass spectrometer...
2017: PloS One
https://www.readbyqxmd.com/read/28725557/genome-assembly-of-chryseobacterium-sp-strain-ihbb-10212-from-glacier-top-surface-soil-in-the-indian-trans-himalayas-with-potential-for-hydrolytic-enzymes
#18
Mohinder Pal, Mohit Kumar Swarnkar, Hena Dhar, Sanjay Chhibber, Arvind Gulati
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas...
September 2017: Genomics Data
https://www.readbyqxmd.com/read/28725229/the-synergistic-priming-effect-of-exogenous-salicylic-acid-and-h2o2-on-chilling-tolerance-enhancement-during-maize-zea-mays-l-seed-germination
#19
Zhan Li, Jungui Xu, Yue Gao, Chun Wang, Genyuan Guo, Ying Luo, Yutao Huang, Weimin Hu, Mohamed S Sheteiwy, Yajing Guan, Jin Hu
Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H2O2) in response to chilling stress, we investigated the effects of seed priming with SA, H2O2, and SA+H2O2 combination on maize resistance under chilling stress (13°C). Priming with SA, H2O2, and especially SA+H2O2 shortened seed germination time and enhanced seed vigor and seedling growth as compared with hydropriming and non-priming treatments under low temperature...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28714845/bacillus-mangrovi-sp-nov-isolated-from-a-sediment-sample-from-a-mangrove-forest
#20
Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities...
July 2017: International Journal of Systematic and Evolutionary Microbiology
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