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amylase gene

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https://www.readbyqxmd.com/read/29108629/spontaneous-pepsin-c-catalyzed-activation-of-human-pepsinogen-c-in-transgenic-rice-cell-suspension-culture-production-and-characterization-of-human-pepsin-c
#1
Md Reyazul Islam, Nan-Sun Kim, Jae-Wan Jung, Hyo-Boon Kim, So-Chon Han, Moon-Sik Yang
A human pepsinogen C (hPGC) gene was synthesized with rice-optimized codon usage and cloned into a rice expression vector containing the promoter, signal peptide, and terminator derived from the rice α-amylase 3D (Ramy3D) gene. In addition, a 6-His tag was added to the 3' end of the synthetic hPGC gene for easy purification. The plant expression vector was introduced into rice calli (Oryza sativa L. cv. Dongjin) mediated by Agrobacterium tumefaciens. The integration of the hPGC gene into the chromosome of the transgenic rice callus and hPGC expression in transgenic rice cell suspensions was verified via genomic DNA polymerase chain reaction amplification and Northern blot analysis...
January 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29071169/draft-genome-sequence-of-a-thermostable-alkaliphilic-%C3%AE-amylase-and-protease-producing-bacillus-amyloliquefaciens-strain-kcp2
#2
Vimalkumar S Prajapati, Sanket Ray, Jitendra Narayan, Chaitanya C Joshi, Kamlesh C Patel, Ujjval B Trivedi, R M Patel
Bacillus amyloliquefaciens strain KCP2 was isolated from municipal food waste samples collected in Vallabh Vidyanagar, Gujarat, India. Strain KCP2 is noteworthy due to its ability to produce a thermostable, alkaliphilic α-amylase and a protease. These enzymes have importance in several industrial processes including bread making, brewing, starch processing, pharmacy, and textile industries. Whole genome sequencing of strain KCP2 showed that the estimated genome size was 3.9 Mb, the G + C content was 46%, and it coded for 4113 genes...
December 2017: 3 Biotech
https://www.readbyqxmd.com/read/29069329/potential-for-plant-biocontrol-activity-of-isolated-pseudomonas-aeruginosa-and-bacillus-stratosphericus-strains-against-bacterial-pathogens-acting-through-both-induced-plant-resistance-and-direct-antagonism
#3
Kaliannan Durairaj, Palanivel Velmurugan, Jung-Hee Park, Woo-Suk Chang, Yool-Jin Park, Palaninaicker Senthilkumar, Kyung-Min Choi, Jeong-Ho Lee, Byung-Taek Oh
Phytopathogenic bacteria have caused significant damage to agricultural crops in both controlled and open cultivation practices, imposing heavy losses to farmers. Thereby, the goal of this study was to evaluate Pseudomonas aeruginosa and Bacillus stratosphericus isolated from soil has antagonistic activity against bacterial phytopathogens with the potential to control plant diseases. Isolated novel strains of P. aeruginosa and B. stratosphericus showed broad spectrum of antagonistic activity against five bacterial phytopathogens...
October 24, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29066669/arabidopsis-%C3%AE-amylase2-is-a-k-requiring-catalytic-tetramer-with-sigmoidal-kinetics
#4
Jonathan D Monroe, Jillian S Breault, Lauren E Pope, Catherine E Torres, Tewaldemedhine B Gebrejesus, Christopher E Berndsen, Amanda R Storm
The Arabidopsis thaliana genome contains nine β-amylase (BAM) genes, some of which play important roles in starch hydrolysis. However, little is known about BAM2, a plastid-localized enzyme reported to have extremely low catalytic activity. Using conservation of intron positions, we determined that the nine Arabidopsis BAM genes fall into two distinct subfamilies. A similar pattern was found in each major lineage of land plants suggesting that these subfamilies diverged prior to the origin of land plants. Moreover, phylogenetic analysis indicated that BAM2 is the ancestral member of one of these subfamilies...
October 24, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29051768/comparisons-of-copy-number-genomic-structure-and-conserved-motifs-for-%C3%AE-amylase-genes-from-barley-rice-and-wheat
#5
Qisen Zhang, Chengdao Li
Barley is an important crop for the production of malt and beer. However, crops such as rice and wheat are rarely used for malting. α-amylase is the key enzyme that degrades starch during malting. In this study, we compared the genomic properties, gene copies, and conserved promoter motifs of α-amylase genes in barley, rice, and wheat. In all three crops, α-amylase consists of four subfamilies designated amy1, amy2, amy3, and amy4. In wheat and barley, members of amy1 and amy2 genes are localized on chromosomes 6 and 7, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29046501/characterization-of-amylolytic-enzyme-overproducing-mutant-of-aspergillus-luchuensis-obtained-by-ion-beam-mutagenesis
#6
Toshihiro Kojo, Chihiro Kadooka, Marisa Komohara, Shiori Onitsuka, Miya Tanimura, Yukiko Muroi, Shugo Kurazono, Yohei Shiraishi, Ken Oda, Kazuhiro Iwashita, Masahira Onoue, Kayu Okutsu, Yumiko Yoshizaki, Kazunori Takamine, Taiki Futagami, Kazuki Mori, Hisanori Tamaki
Aspergillus luchuensis is a kuro (black) koji fungus that has been used as a starch degrader for the awamori- and shochu-making industries in Japan. In this study, we investigated the effect of ion beam irradiation on A. luchuensis RIB2601 and obtained a high starch-degrading mutant strain U1. Strain U1 showed reduced growth rate, whereas it showed higher α-amylase, glucoamylase, and α-glucosidase activities on a mycelial mass basis than the wild type (wt) strain both on agar plates and in rice koji. In addition, strain U1 showed higher N-acetylglucosamine content in the cell wall and higher sensitivity to calcofluor white, suggesting a deficiency in cell wall composition...
October 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29045415/isolation-identification-and-phenotypic-and-molecular-characterization-of-pathogenic-vibrio-vulnificus-isolated-from-litopenaeus-vannamei
#7
Tao Teng, Liguo Liang, Kai Chen, Bingwen Xi, Jun Xie, Pao Xu
The morphology and the drug sensitivity of the strain GYX2014-1 isolated from the hepatic pancreatic tissue of moribund Litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. Detection of extracellulase and hemolysin activity shows that the isolated GYX2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. The 16S rRNA gene (GenBank accession number: KT781675) was analyzed, and a phylogenetic tree analysis showed that the isolated pathogen was most closely related to V...
2017: PloS One
https://www.readbyqxmd.com/read/29037836/the-genomic-and-transcriptomic-basis-of-the-potential-of-lactobacillus-plantarum-a6-to-improve-the-nutritional-quality-of-a-cereal-based-fermented-food
#8
Williams Turpin, Marion Weiman, Jean-Pierre Guyot, Aurélie Lajus, Stéphane Cruveiller, Christèle Humblot
The objective of this work was to investigate the nutritional potential of Lactobacillus plantarum A6 in a food matrix using next generation sequencing. To this end, we characterized the genome of the A6 strain for a complete overview of its potential. We then compared its transcriptome when grown in a food matrix made from pearl millet to and its transcriptome when cultivated in a laboratory medium. Genomic comparison of the strain L. plantarum A6 with the strains WCFS1, ST-III, JDM1 and ATCC14917 led to the identification of five regions of genomic plasticity...
October 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29030997/rna-interference-and-functional-characterization-of-a-tergal-gland-alpha-amylase-in-the-german-cockroach-blattella-germanica-l
#9
A J Myers, A D Gondhalekar, M Fardisi, K D Pluchar, K D Saltzmann, G W Bennett, M E Scharf
German cockroach males possess tergal glands that secrete a combination of oligosaccharides, lipids and proteins. Four major proteins occur in the secretion, with one being the 63 kDa alpha-amylase Blattella germanica Tergal Gland protein-1 (BGTG-1). Denaturing and starch gel electrophoresis coupled with peptide sequencing verified amylase activity for the BGTG-1 protein. BGTG-1 gene expression profiles were determined by using quantitative real-time PCR to compare messenger RNA abundance among isolated tissues of males, females and gravid females...
October 14, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29030601/interfering-with-the-high-affinity-interaction-between-wheat-amylase-trypsin-inhibitor-cm3-and-toll-like-receptor-4-in-silico-and-biosensor-based-studies
#10
Massimiliano Cuccioloni, Matteo Mozzicafreddo, Laura Bonfili, Valentina Cecarini, Mara Giangrossi, Maurizio Falconi, Shin-Ichiroh Saitoh, Anna Maria Eleuteri, Mauro Angeletti
Wheat amylase/trypsin bi-functional inhibitors (ATIs) are protein stimulators of innate immune response, with a recently established role in promoting both gastrointestinal and extra-gastrointestinal inflammatory syndromes. These proteins have been reported to trigger downstream intestinal inflammation upon activation of TLR4, a member of the Toll-like family of proteins that activates signalling pathways and induces the expression of immune and pro-inflammatory genes. In this study, we demonstrated the ability of ATI to directly interact with TLR4 with nanomolar affinity, and we kinetically and structurally characterized the interaction between these macromolecules by means of a concerted approach based on surface plasmon resonance binding analyses and computational studies...
October 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29029703/comparative-genomics-of-maize-ear-rot-pathogens-reveals-expansion-of-carbohydrate-active-enzymes-and-secondary-metabolism-backbone-genes-in-stenocarpella-maydis
#11
Alex Z Zaccaron, Charles P Woloshuk, Burton H Bluhm
Stenocarpella maydis is a plant pathogenic fungus that causes Diplodia ear rot, one of the most destructive diseases of maize. To date, little information is available regarding the molecular basis of pathogenesis in this organism, in part due to limited genomic resources. In this study, a 54.8 Mb draft genome assembly of S. maydis was obtained with Illumina and PacBio sequencing technologies, and analyzed. Comparative genomic analyses with the predominant maize ear rot pathogens Aspergillus flavus, Fusarium verticillioides, and Fusarium graminearum revealed an expanded set of carbohydrate-active enzymes for cellulose and hemicellulose degradation in S...
November 2017: Fungal Biology
https://www.readbyqxmd.com/read/29023967/copper-toxicity-and-date-palm-seedling-tolerance-monitoring-of-related-biomarkers
#12
Zayneb ChaÂBene, Imen Rekik Hakim, Agnieszka Rorat, Amine Elleuch, Hafedh Mejdoub, Franck Vandenbulcke
Date palm seeds were exposed to different copper solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas, higher Cu amount (2 mM) significantly inhibited seeds germination, delayed hypocotyl elongation, increased seedling mortality and reduced germination index by more than 90 %. Metal-related toxicity symptoms appeared after 15 days of 2 mM Cu exposure. Biochemical activities, like amylase activity and redox balance elements were examined in order to study the relationship between external Cu amount and internal plant response...
October 12, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28993954/obesity-alleviating-potential-of-asiatic-acid-and-its-effects-on-acc1-ucp2-and-cpt1-mrna-expression-in-high-fat-diet-induced-obese-sprague-dawley-rats
#13
P Rameshreddy, V V Sathibabu Uddandrao, Parim Brahmanaidu, S Vadivukkarasi, Ramavat Ravindarnaik, Pothani Suresh, K Swapna, A Kalaivani, Parimi Parvathi, P Tamilmani, Ganapathy Saravanan
The present study evaluated the effects of asiatic acid (AA), a pentacyclic triterpenoid from Centella asiatica on lipid metabolism parameters in a rat model of obesity induced using a high fat diet (HFD) for 42 days. AA (20 mg/kg body weight [BW]) was administered orally once daily for 42 days, and an orlistat-treated group of rats (10 mg/kg BW) was included for comparison. Changes in BW, blood glucose levels, insulin resistance and leptin, adiponectin, amylase, and lipase levels in the blood; lipid profiles of plasma; liver antioxidants levels; and acetyl CoA carboxylase(ACC), uncoupling protein-2 (UCP2), and carnitine palmitoyltransferase-1 (CPT1) mRNA expression were observed in the experimental rats...
October 9, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28986243/construction-of-an-expression-vector-that-uses-the-aph-promoter-for-protein-expression-in-corynebacterium-glutamicum
#14
Wei Zhang, Zihao Zhao, Yankun Yang, Xiuxia Liu, Zhonghu Bai
Corynebacterium glutamicum is an attractive host for the production of heterologous proteins despite its traditional use in fermentative production of amino acids. To enhance the expression levels of target genes, the development of useful promoters is required in the construction of expression systems. Here, we developed a new promoter, the aph promoter from aminoglycoside-3'-phosphotransferase gene, and used it to construct monocistronic and bicistronic expression systems that host different ribosome binding site (RBS) sequences...
October 3, 2017: Plasmid
https://www.readbyqxmd.com/read/28973750/genetic-diversity-of-bacillus-sp-producers-of-amylase-isolated-from-the-soil
#15
A R E O Xavier, E R Lima, A M E Oliveira, L Cardoso, J Santos, C H C Cangussu, L N Leite, M C L Quirino, I G C Júnior, D A Oliveira, M A S Xavier
The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyze the genetic diversity among bacteria of the genus Bacillus sp producer of amylase originated from the soil. To this end, soil samples were collected and submitted to the condition of extreme temperature...
September 27, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28970110/increased-production-of-biomass-degrading-enzymes-by-double-deletion-of-crea-and-creb-genes-involved-in-carbon-catabolite-repression-in-aspergillus-oryzae
#16
Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
In a previous study, we reported that a double gene deletion mutant for CreA and CreB, which constitute the regulatory machinery involved in carbon catabolite repression, exhibited improved production of α-amylase compared with the wild-type strain and single creA or creB deletion mutants in Aspergillus oryzae. Because A. oryzae can also produce biomass-degrading enzymes, such as xylolytic and cellulolytic enzymes, we examined the production levels of those enzymes in deletion mutants in this study. Xylanase and β-glucosidase activities in the wild-type were hardly detected in submerged culture containing xylose as the carbon source, whereas those enzyme activities were significantly increased in the single creA deletion (ΔcreA) and double creA and creB deletion (ΔcreAΔcreB) mutants...
September 29, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28966889/diversity-of-cultivated-aerobic-poly-hydrolytic-bacteria-in-saline-alkaline-soils
#17
Dimitry Y Sorokin, Tatiana V Kolganova, Tatiana V Khijniak, Brian E Jones, Ilya V Kublanov
Alkaline saline soils, known also as "soda solonchaks", represent a natural soda habitat which differs from soda lake sediments by higher aeration and lower humidity. The microbiology of soda soils, in contrast to the more intensively studied soda lakes, remains poorly explored. In this work we investigate the diversity of culturable aerobic haloalkalitolerant bacteria with various hydrolytic activities from soda soils at different locations in Central Asia, Africa, and North America. In total, 179 pure cultures were obtained by using media with various polymers at pH 10 and 0...
2017: PeerJ
https://www.readbyqxmd.com/read/28965975/metabolic-engineering-of-aspergillus-oryzae-for-efficient-production-of-l-malate-directly-from-corn-starch
#18
Jingjing Liu, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
l-Malate, an important chemical building block, has been widely applied in the food, pharmaceutical, and bio-based materials industries. In previous work, we engineered Aspergillus oryzae by rewiring the reductive tricarboxylic acid pathway to produce l-malate from glucose. To decrease the production cost, here, we further engineered A. oryzae to efficiently produce l-malate directly from corn starch with simultaneous liquefaction-saccharification and fermentation. First, a promoter PN5 was constructed by quintuple tandem of the 97-bp fragment containing the cis-element of the glucoamylase gene promoter (PglaA), and with the promoter PN5, the transcriptional level of glaA gene increased by 25-45%...
September 28, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28955633/effect-of-differential-processing-of-the-native-and-recombinant-%C3%AE-amylase-from-bacillus-amyloliquefaciens-jjc33m-on-specificity-and-enzyme-properties
#19
Juan José Montor-Antonio, Sarahi Hernández-Heredia, Ángela Ávila-Fernández, Clarita Olvera, Bernardo Sachman-Ruiz, Sandra Del Moral
AmyJ33, an α-amylase isolated from Bacillus amyloliquefaciens JJC33M, has been characterized as a non-metalloenzyme that hydrolyzes raw starch. In this work, the gene that codifies for AmyJ33 was isolated and cloned. The recombinant α-amylase (AmyJ33r) produced had a molecular weight of 72 kDa, 25 kDa higher than the native α-amylase (AmyJ33). Our results suggest that the C-terminal was processed in a different way in the native and the recombinant enzyme causing the difference observed in the molecular weight...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28936221/metabolite-profiling-of-barley-grains-subjected-to-water-stress-to-explain-the-genotypic-difference-in-drought-induced-impacts-on-malting-quality
#20
Xiaojian Wu, Kangfeng Cai, Guoping Zhang, Fanrong Zeng
Grain weight and protein content will be reduced and increased, respectively, when barley is subjected to water stress after anthesis, consequently deteriorating the malt quality. However, such adverse impact of water stress differs greatly among barley genotypes. In this study, two Tibetan wild barley accessions and two cultivated varieties differing in water stress tolerance were used to investigate the genotypic difference in metabolic profiles during grain-filling stage under drought condition. Totally, 71 differently accumulated metabolites were identified, including organic acids, amino acids/amines, and sugars/sugar alcohols...
2017: Frontiers in Plant Science
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