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https://www.readbyqxmd.com/read/28332853/correlations-between-lp-pla2-gene-polymorphisms-and-susceptibility-and-severity-of-acute-pancreatitis-in-a-chinese-population
#1
Min Ma, Chun-Xia Zhai, Cai-Xia Sun
OBJECTIVE: This case-control study explored correlations between LP-PLA2 gene polymorphisms (A379V, V279F, and R92H) and susceptibility and severity of acute pancreatitis (AP) in a Chinese population. MATERIALS AND METHODS: From October 2013 to October 2015, 94 AP patients were chosen as the case group. According to the Acute Physiology and Chronic Health Evaluation (APACHE) II score standard, AP patients were divided into a mild AP (MAP) group (n = 46) and severe AP (SAP) group (n = 48)...
March 23, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28330224/transcriptome-wide-mining-suggests-conglomerate-of-genes-associated-with-tuberous-root-growth-and-development-in-aconitum-heterophyllum-wall
#2
Nikhil Malhotra, Hemant Sood, Rajinder Singh Chauhan
Tuberous roots of Aconitum heterophyllum constitute storage organ for secondary metabolites, however, molecular components contributing to their formation are not known. The transcriptomes of A. heterophyllum were analyzed to identify possible genes associated with tuberous root development by taking clues from genes implicated in other plant species. Out of 18 genes, eight genes encoding GDP-mannose pyrophosphorylase (GMPase), SHAGGY, Expansin, RING-box protein 1 (RBX1), SRF receptor kinase (SRF), β-amylase, ADP-glucose pyrophosphorylase (AGPase) and Auxin responsive factor 2 (ARF2) showed higher transcript abundance in roots (13-171 folds) compared to shoots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28323908/effects-of-protease-and-non-starch-polysaccharide-enzyme-on-performance-digestive-function-activity-and-gene-expression-of-endogenous-enzyme-of-broilers
#3
Lin Yuan, Mingfa Wang, Xiaotu Zhang, Zhixiang Wang
Three hundred one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets containing non-starch polysaccharide (NSP) enzyme and different levels of acid protease from 1 to 42 days of age to investigate the effects of exogenous enzymes on growth performance, digestive function, activity of endogenous digestive enzymes in the pancreas and mRNA expression of pancreatic digestive enzymes. For days 1-42, compared to the control chickens, average daily feed intake (ADFI) and average daily gain (ADG) were significantly enhanced by the addition of NSP enzyme in combination with protease supplementation at 40 or 80 mg/kg (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28317043/il-8-gene-polymorphism-in-acute-biliary-and-non-biliary-pancreatitis-probable-cause-of-high-level-parameters
#4
Ender Anilir, Filiz Ozen, Ibrahim Halil Yildirim, Ibrahim Ali Ozemir, Can Ozlu, Orhan Alimoglu
BACKGROUNDS/AIMS: Inflammatory mediators of the innate immune response play fundamental roles in the pathogenesis of acute pancreatitis. The correlation between interleukin-8 (IL-8) gene polymorphism with types of acute pancreatitis and severity of pancreatitis, was evaluated in this study. METHODS: According to the diagnostic criteria, 176 patients with acute pancreatitis were grouped into biliary (n=83) and nonbiliary pancreatitis (n=93). Healthy blood donors (n=100) served as controls...
February 2017: Ann Hepatobiliary Pancreat Surg
https://www.readbyqxmd.com/read/28314965/genetic-analyses-using-gge-model-and-a-mixed-linear-model-approach-and-stability-analyses-using-ammi-bi-plot-for-late-maturity-alpha-amylase-activity-in-bread-wheat-genotypes
#5
Golam Rasul, Karl D Glover, Padmanaban G Krishnan, Jixiang Wu, William A Berzonsky, Bourlaye Fofana
Low falling number and discounting grain when it is downgraded in class are the consequences of excessive late-maturity α-amylase activity (LMAA) in bread wheat (Triticum aestivum L.). Grain expressing high LMAA produces poorer quality bread products. To effectively breed for low LMAA, it is necessary to understand what genes control it and how they are expressed, particularly when genotypes are grown in different environments. In this study, an International Collection (IC) of 18 spring wheat genotypes and another set of 15 spring wheat cultivars adapted to South Dakota (SD), USA were assessed to characterize the genetic component of LMAA over 5 and 13 environments, respectively...
March 17, 2017: Genetica
https://www.readbyqxmd.com/read/28303149/qtl-hotspots-for-early-vigor-and-related-traits-under-dry-direct-seeded-system-in-rice-oryza-sativa-l
#6
Uma M Singh, Shailesh Yadav, Shilpi Dixit, P Janaki Ramayya, M Nagamallika Devi, K Anitha Raman, Arvind Kumar
Strong seedling vigor is desirable trait in dry direct-seeded rice (DSR) for enhancing crop establishment and the ability to compete against weeds. A set of 253 BC3F4 lines derived from cross between Swarna and Moroberekan was phenotyped for early vigor (EV) and 8 related traits viz., early uniform emergence (EUE), shoot length (SHL), stem length (SL), shoot fresh weight (SFW), total fresh weight (TFW), shoot dry weight (SDW), total dry weight (TDW), and root dry weight (RDW). Composite interval mapping analysis using genotypic data from 194 SNP markers identified six genomic regions associated with traits on chromosomes 3, 4, 5, and 6 with phenotypic variance ranging from 2...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28301987/properties-of-epithelial-cells-and-vaginal-secretions-in-pregnant-women-when-lactobacillus-crispatus-or-lactobacillus-iners-dominate-the-vaginal-microbiome
#7
Julie Leizer, Dimitrios Nasioudis, Larry J Forney, G Maria Schneider, Karol Gliniewicz, Allison Boester, Steven S Witkin
OBJECTIVE: Our objective was to determine differences in properties of vaginal epithelial cells and the composition of vaginal secretions when Lactobacillus crispatus or Lactobacillus iners are numerically dominant in the vaginal microenvironment of pregnant women. METHODS: The vaginal microbiomes of 157 first-trimester pregnant women were identified by classifying partial 16S gene sequences amplified from the V1 to V3 region of bacterial ribosomal 16S RNA genes...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28251549/the-hyperthermophilic-%C3%AE-amylase-from-thermococcus-sp-hj21-does-not-require-exogenous-calcium-for-thermostability-because-of-high-binding-affinity-to-calcium
#8
Huaixu Cheng, Zhidan Luo, Mingsheng Lu, Song Gao, Shujun Wang
The hyperthermophilic α-amylase from Thermococcus sp. HJ21 does not require exogenous calcium ions for thermostability, and is a promising alternative to commercially available α-amylases to increase the efficiency of industrial processes like the liquefaction of starch. We analyzed the amino acid sequence of this α-amylase by sequence alignments and structural modeling, and found that this α-amylase closely resembles the α-amylase from Pyrococcus woesei. The gene of this α-amylase was cloned in Escherichia coli and the recombinant α-amylase was overexpressed and purified with a combined renaturation-purification procedure...
March 1, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28238910/engineering-a-chimeric-acid-stable-%C3%AE-amylase-glucoamylase-amy-glu-for-one-step-starch-saccharification
#9
Deepak Parashar, T Satyanarayana
For saccharifying starch in one step, a chimeric biocatalyst (Amy-Glu) was generated from engineered α-amylase (Ba-Gt-amy) of Bacillus acidicola and glucoamylase (Glu) gene of Aspergillus niger. In order to join two enzymes, a linker peptide of 25 amino acids was used. Chimeric Amy-Glu was expressed in E. coli. Glu is of 75kDa, while Amy-Glu is of 145kDa. Both Amy-Glu and Glu displayed similar pH profile with good activity in the acidic pH range like that of Ba-Gt-amy with optimum at pH 4.0. All three enzymes (Ba-Gt-amy, Amy-Glu and glucoamylase) exhibited activity in the temperature range between 40 and 70°C with optimum at 60°C...
February 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28236189/amylolytic-enzymes-acquired-from-l-lactic-acid-producing-enterococcus-faecium-k-1-and-improvement-of-direct-lactic-acid-production-from-cassava-starch
#10
Kridsada Unban, Apinun Kanpiengjai, Goro Takata, Keiko Uechi, Wen-Chien Lee, Chartchai Khanongnuch
An amylolytic lactic acid bacterium isolate K-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as Entercoccus faecium based on 16S rRNA gene sequence analysis. An extracellular α-amylase was purified to homogeneity and the molecular weight of the purified enzyme was approximately 112 kDa with optimal pH value and temperature measured of 7.0 and 40 °C, respectively. It was stable at a pH range of 6.0-7.0, but was markedly sensitive to high temperatures and low pH conditions, even at a pH value of 5...
February 24, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28219410/copy-number-variation-of-human-amy1-is-a-minor-contributor-to-variation-in-salivary-amylase-expression-and-activity
#11
Danielle Carpenter, Laura M Mitchell, John A L Armour
BACKGROUND: Salivary amylase in humans is encoded by the copy variable gene AMY1 in the amylase gene cluster on chromosome 1. Although the role of salivary amylase is well established, the consequences of the copy number variation (CNV) at AMY1 on salivary amylase protein production are less well understood. The amylase gene cluster is highly structured with a fundamental difference between odd and even AMY1 copy number haplotypes. In this study, we aimed to explore, in samples from 119 unrelated individuals, not only the effects of AMY1 CNV on salivary amylase protein expression and amylase enzyme activity but also whether there is any evidence for underlying difference between the common haplotypes containing odd numbers of AMY1 and even copy number haplotypes...
February 20, 2017: Human Genomics
https://www.readbyqxmd.com/read/28212656/using-a-vector-pool-containing-variable-strength-promoters-to-optimize-protein-production-in-yarrowia-lipolytica
#12
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
BACKGROUND: The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2...
February 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28197133/metatranscriptomics-reveals-the-active-bacterial-and-eukaryotic-fibrolytic-communities-in-the-rumen-of-dairy-cow-fed-a-mixed-diet
#13
Sophie Comtet-Marre, Nicolas Parisot, Pascale Lepercq, Frédérique Chaucheyras-Durand, Pascale Mosoni, Eric Peyretaillade, Ali R Bayat, Kevin J Shingfield, Pierre Peyret, Evelyne Forano
Ruminants have a unique ability to derive energy from the degradation of plant polysaccharides through the activity of the rumen microbiota. Although this process is well studied in vitro, knowledge gaps remain regarding the relative contribution of the microbiota members and enzymes in vivo. The present study used RNA-sequencing to reveal both the expression of genes encoding carbohydrate-active enzymes (CAZymes) by the rumen microbiota of a lactating dairy cow and the microorganisms forming the fiber-degrading community...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28193788/erratum-metabolomic-profile-of-low-copy-number-carriers-at-the-salivary-%C3%AE-amylase-gene-suggests-a-metabolic-shift-toward-lipid-based-energy-production-diabetes-2016-65-3362-3368
#14
Abdelilah Arredouani, Matteo Stocchero, Nicola Culeddu, Julia El-Sayed Moustafa, Jean Tichet, Beverley Balkau, Thierry Brousseau, Marco Manca, Mario Falchi
No abstract text is available yet for this article.
February 13, 2017: Diabetes
https://www.readbyqxmd.com/read/28187329/genetic-and-biochemical-characterization-of-an-oligo-%C3%AE-1-6-glucosidase-from-lactobacillus-plantarum
#15
Susana Delgado, Ana Belén Flórez, Lucía Guadamuro, Baltasar Mayo
Although encoded in the genome of many Lactobacillus spp. strains, α-glucosidases have received little attention compared to other glycosyl hydrolases. In this study, a putative oligosaccharide(oligo)-α-1,6-glucosidase-encoding gene (malL) was identified in the genome of Lactobacillus plantarum LL441. malL coded for 572 amino acid residues with a calculated total molecular mass of 66.31kDa. No predicted signal peptide was observed, suggesting this enzyme to be localized within the cytoplasm of the cell. Homology studies of the deduced amino acid sequence in the area of its active sites classified the enzyme as a member of the α-amylase (AmyAC) superfamily of glycosyl hydrolases (GH), family 13 (GH13), subfamily 31 (GH13_31)...
February 1, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28143661/novel-therapy-for-pancreatic-fistula-using-adipose-derived-stem-cell-sheets-treated-with-mannose
#16
Hirokazu Kaneko, Toshio Kokuryo, Yukihiro Yokoyama, Junpei Yamaguchi, Tokunori Yamamoto, Rei Shibata, Momokazu Gotoh, Toyoaki Murohara, Akira Ito, Masato Nagino
BACKGROUND: Given that no studies have reported the use of adipose-derived stem cell sheets for the prevention of pancreatic fistulas, it is unclear whether adipose-derived stem cell sheets are effective at preventing this complication. The aim of this study was to evaluate the efficacy of novel therapy for the prevention of pancreatic fistulas using adipose-derived stem cell sheets treated with mannose. METHODS: The rat pancreatic duct (splenic duct) and surrounding pancreatic parenchyma were transected to induce a pancreatic fistula...
January 28, 2017: Surgery
https://www.readbyqxmd.com/read/28125683/differences-in-amy1-gene-copy-numbers-derived-from-blood-buccal-cells-and-saliva-using-quantitative-and-droplet-digital-pcr-methods-flagging-the-pitfall
#17
Delicia Shu Qin Ooi, Verena Ming Hui Tan, Siong Gim Ong, Yiong Huak Chan, Chew Kiat Heng, Yung Seng Lee
INTRODUCTION: The human salivary (AMY1) gene, encoding salivary α-amylase, has variable copy number variants (CNVs) in the human genome. We aimed to determine if real-time quantitative polymerase chain reaction (qPCR) and the more recently available Droplet Digital PCR (ddPCR) can provide a precise quantification of the AMY1 gene copy number in blood, buccal cells and saliva samples derived from the same individual. METHODS: Seven participants were recruited and DNA was extracted from the blood, buccal cells and saliva samples provided by each participant...
2017: PloS One
https://www.readbyqxmd.com/read/28109854/histone-methyltransferase-aflrmta-gene-is-involved-in-the-morphogenesis-mycotoxin-biosynthesis-and-pathogenicity-of-aspergillus-flavus
#18
Yu Li, Yizi He, Xing Li, Opemipo Esther Fasoyin, Yule Hu, Yaju Liu, Jun Yuan, Zhenhong Zhuang, Shihua Wang
Arginine methyltransferases catalyze the posttranslational methylation of arginine, which is involved in a range of important biological processes. aflrmtA gene, an arginine methyltransferase was deleted from Aspergillus flavus in this study by homologous recombination. In morphogenesis assay, aflrmtA was found to down-regulate conidiation by regulating the activity of brlA and abaA genes. It was also found to increase sclerotia formation by up-regulating the expression of nsdC and nsdD genes. In mycotoxin biosynthesis, aflrmtA gene was found to significantly up-regulate the biosynthesis of AFB1 in PDA and PDB media by improving the expression of aflR, aflC and aflK, but it was of no effect in YES medium...
January 19, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28101908/recurrent-rearrangements-of-human-amylase-genes-create-multiple-independent-cnv-series
#19
Nzar A A Shwan, Sandra Louzada, Fengtang Yang, John A L Armour
The human amylase gene cluster includes the human salivary (AMY1) and pancreatic amylase genes (AMY2A and AMY2B), and is a highly variable and dynamic region of the genome. Copy number variation of AMY1 has been implicated in human dietary adaptation, and in population association with obesity, but neither of these findings has been independently replicated. Despite these functional implications, the structural genomic basis of copy number variation (CNV) has only been defined in detail very recently. In this work we use high-resolution analysis of copy number, and analysis of segregation in trios, to define new, independent allelic series of amylase CNVs in sub-Saharan Africans, including a series of higher-order expansions of a unit consisting of one copy each of AMY1, AMY2A and AMY2B...
January 19, 2017: Human Mutation
https://www.readbyqxmd.com/read/28095353/liver-alpha-amylase-gene-expression-as-an-early-obesity-biomarker
#20
Marzieh Mojbafan, Zohreh Afsartala, Mahsa M Amoli, Mahdi Mahmoudi, Parichehreh Yaghmaei, Bagher Larijani, Azadeh Ebrahim-Habibi
BACKGROUND: Obesity is a major health problem worldwide, for which preventive and therapeutic means are still needed. Alpha-amylase is a digestive enzyme whose inhibition has been targeted as a potential anti-obesity strategy. However, alpha-amylase gene expression has not been particularly attended to, and in contrast with pancreatic and salivary amylases, fewer studies have focused on liver alpha-amylase. The present study aimed at investigating the expression of alpha-amylase gene in obese and normal mice at RNA and protein level as well as acarbose effect on this gene expression in hepatocyte cell culture...
April 2017: Pharmacological Reports: PR
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