keyword
MENU ▼
Read by QxMD icon Read
search

amylase gene

keyword
https://www.readbyqxmd.com/read/28729759/transcriptome-wide-identification-and-characterization-of-starch-branching-enzyme-in-finger-millet
#1
Rajhans Tyagi, Apoorv Tiwari, Vijay Kumar Garg, Sanjay Gupta
Starch-branching enzymes (SBEs) are one of the four major enzyme classes involved in starch biosynthesis in plants and play an important role in determining the structure and physical properties of starch granules. Multiple SBEs are involved in starch biosynthesis in plants. Finger millet is calcium rich important serial crop belongs to grass family and the transcriptome data of developing spikes is available on NCBI. In this study it was try to find out the gene sequence of starch branching enzyme and annotate the sequence and submit the sequence for further use...
2017: Bioinformation
https://www.readbyqxmd.com/read/28727805/turgor-responsive-starch-phosphorylation-in-oryza-sativa-stems-a-primary-event-of-starch-degradation-associated-with-grain-filling-ability
#2
Hiroshi Wada, Chisato Masumoto-Kubo, Koichi Tsutsumi, Hiroshi Nonami, Fukuyo Tanaka, Haruka Okada, Rosa Erra-Balsells, Kenzo Hiraoka, Taiken Nakashima, Makoto Hakata, Satoshi Morita
Grain filling ability is mainly affected by the translocation of carbohydrates generated from temporarily stored stem starch in most field crops including rice (Oryza sativa L.). The partitioning of non-structural stem carbohydrates has been recognized as an important trait for raising the yield ceiling, yet we still do not fully understand how carbohydrate partitioning occurs in the stems. In this study, two rice subspecies that exhibit different patterns of non-structural stem carbohydrates partitioning, a japonica-dominant cultivar, Momiroman, and an indica-dominant cultivar, Hokuriku 193, were used as the model system to study the relationship between turgor pressure and metabolic regulation of non-structural stem carbohydrates, by combining the water status measurement with gene expression analysis and a dynamic prefixed 13C tracer analysis using a mass spectrometer...
2017: PloS One
https://www.readbyqxmd.com/read/28725557/genome-assembly-of-chryseobacterium-sp-strain-ihbb-10212-from-glacier-top-surface-soil-in-the-indian-trans-himalayas-with-potential-for-hydrolytic-enzymes
#3
Mohinder Pal, Mohit Kumar Swarnkar, Hena Dhar, Sanjay Chhibber, Arvind Gulati
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas...
September 2017: Genomics Data
https://www.readbyqxmd.com/read/28725229/the-synergistic-priming-effect-of-exogenous-salicylic-acid-and-h2o2-on-chilling-tolerance-enhancement-during-maize-zea-mays-l-seed-germination
#4
Zhan Li, Jungui Xu, Yue Gao, Chun Wang, Genyuan Guo, Ying Luo, Yutao Huang, Weimin Hu, Mohamed S Sheteiwy, Yajing Guan, Jin Hu
Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H2O2) in response to chilling stress, we investigated the effects of seed priming with SA, H2O2, and SA+H2O2 combination on maize resistance under chilling stress (13°C). Priming with SA, H2O2, and especially SA+H2O2 shortened seed germination time and enhanced seed vigor and seedling growth as compared with hydropriming and non-priming treatments under low temperature...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28714845/bacillus-mangrovi-sp-nov-isolated-from-a-sediment-sample-from-a-mangrove-forest
#5
Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28709869/protective-effect-of-bacillopeptidase-cfr5-from-bacillus-subtilis-cfr5-on-cerulein-induced-pancreatitis
#6
G R Sharmila, G Venkateswaran
Bacillopeptidase is a serine peptidase, known for its fibrinolytic activity. However, a very little information is known about its in vivo inflammatory and/or anti-inflammatory properties. Thus, to understand whether bacillopeptidase incorporation can regulate pancreatitis or not, the cerulein-induced pancreatitis model was used, and the role of bacillopeptidase on pancreatitis was studied. In this study, 46 kDa protein was purified from Bacillus subtilis and identified as bacillopeptidase CFR5 (BPC) through MS/MS analysis...
July 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28684177/improvement-of-cellulolytic-enzyme-production-and-performance-by-rational-designing-expression-regulatory-network-and-enzyme-system-composition
#7
REVIEW
Zhonghai Li, Guodong Liu, Yinbo Qu
Filamentous fungi are considered as the most efficient producers expressing lignocellulose-degrading enzymes. Penicillium oxalicum strains possess extraordinary fungal lignocellulolytic enzyme systems and can efficiently utilize plant biomass. In recent years, the regulatory aspects of production of hydrolytic enzymes by P. oxalicum have been well established. This review aims to discuss the recent developments for the production of lignocellulolytic enzymes by P. oxalicum. The main cellulolytic transcription factors mediating the complex transcriptional-regulatory network are highlighted...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28683528/isolation-and-characterization-of-some-promoter-sequences-from-leuconostoc-mesenteroides-sy2-isolated-from-kimchi
#8
Ji Yeong Park, Seon-Ju Jeong, Jeong A Kim, Jeong Hwan Kim
Some promoters were isolated and characterized from genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI cut pBV5030, a promoter screening vector containing a promoter-less cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for 3 putative promoters (P04C, P25C, and P33C) by primer extension...
July 7, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28678349/construction-of-a-multi-control-sterility-system-for-a-maize-male-sterile-line-and-hybrid-seed-production-based-on-the-zmms7-gene-encoding-a-phd-finger-transcription-factor
#9
Danfeng Zhang, Suowei Wu, Xueli An, Ke Xie, Yan Zhou, Liwen Xu, Wen Fang, Shensi Liu, Shuangshuang Liu, Taotao Zhu, Jinping Li, Liqun Rao, Jiuran Zhao, Xiangyuan Wan
Although hundreds of genetic male sterility (GMS) mutants have been identified in maize, few are commercially used due to a lack of effective methods to produce large quantities of pure male-sterile seeds. Here, we develop a multi-control sterility (MCS) system based on the maize male sterility 7 (ms7) mutant and its wild-type Zea mays Male sterility 7 (ZmMs7) gene via a transgenic strategy, leading to the utilization of GMS in hybrid seed production. ZmMs7 is isolated by a map-based cloning approach, and encodes a PHD-finger transcription factor orthologous to rice PTC1 and Arabidopsis MS1...
July 5, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28662104/vibrio-aphrogenes-sp-nov-in-the-rumoiensis-clade-isolated-from-a-seaweed
#10
Mami Tanaka, Shoko Endo, Fumihito Kotake, Nurhidayu Al-Saari, A K M Rohul Amin, Gao Feng, Sayaka Mino, Hidetaka Doi, Yoshitoshi Ogura, Tetsuya Hayashi, Wataru Suda, Masahira Hattori, Isao Yumoto, Toko Sawabe, Tomoo Sawabe, Toshiyoshi Araki
A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio...
2017: PloS One
https://www.readbyqxmd.com/read/28659346/starch-digestion-related-amylase-genetic-variant-affects-2-year-changes-in-adiposity-in-response-to-weight-loss-diets-the-pounds-lost-trial
#11
Yoriko Heianza, Dianjianyi Sun, Tiange Wang, Tao Huang, George A Bray, Frank M Sacks, Lu Qi
Salivary and pancreatic amylases (encoded by AMY1 and AMY2 genes, respectively) are responsible for digesting starchy foods. AMY1 and AMY2 show copy number variations which affect differences in amylase amount and activity, and it is suggested that AMY1 copies are associated with adiposity. We investigated whether genetic variants determining amylase gene copies were associated with 2-year changes of adiposity among 692 overweight and obese individuals who were randomly assigned to diets varying in macronutrient contents...
June 28, 2017: Diabetes
https://www.readbyqxmd.com/read/28655353/xc_0531-encodes-a-c-type-cytochrome-biogenesis-protein-and-is-required-for-pathogenesis-in-xanthomonas-campestris-pv-campestris
#12
Lei Chen, Mingpeng Wang, Li Huang, Zhaojie Zhang, Fanghua Liu, Guangtao Lu
BACKGROUND: The phytopathogenic Xanthomonas campestris pv.campestris is a gram-negative bacterium and the causal agent of black-rot disease of cruciferous crops. Many gram-negative bacteria possess a family of proteins, called Dsbs, which are involved in disulfide bond formation in certain periplasmic proteins. In our preliminary screening of the virulence to the plants we identified that gene XC_0531 which annotated gene dsbD of Xanthomonas campestris pv. campestris (Xcc) is related to the virulence to the host plants...
June 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28648523/functional-characterization-and-crystal-structure-of-thermostable-amylase-from-thermotoga-petrophila-reveals-high-thermostability-and-an-unusual-form-of-dimerization
#13
Uzma Hameed, Ian Price, Ikram-Ul-Haq, Ailong Ke, David B Wilson, Osman Mirza
Thermostable α-amylases have many industrial applications and are therefore continuously explored from novel sources. We present the characterization of a novel putative α-amylase gene product (Tp-AmyS) cloned from Thermotoga petrophila. The purified recombinant enzyme is highly thermostable and able to hydrolyze starch into dextrin between 90 and 100°C, with optimum activity at 98°C and pH8.5. The activity increased in the presence of Rb(1+), K(1+) and Ca(2+) ions, whereas other ions inhibited activity...
June 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28648518/selective-inhibition-of-bet-proteins-reduces-pancreatic-damage-and-systemic-inflammation-in-bile-acid-and-fatty-acid-ethyl-ester-but-not-caerulein-induced-acute-pancreatitis-in-mice
#14
Wei Huang, Andrea C Haynes, Rajarshi Mukherjee, Li Wen, Diane Latawiec, Alexei V Tepikin, David N Criddle, Rab K Prinjha, Nicholas Smithers, Robert Sutton
OBJECTIVES: To evaluate the therapeutic potential of I-BET-762, an inhibitor of the bromodomain and extra-terminal (BET) protein family, in experimental acute pancreatitis (AP). METHODS: AP was induced in mice by retrograde infusion of taurolithocholic acid sulphate into the biliopancreatic duct (TLCS-AP) or two intraperitoneal (i.p.) injections of ethanol and palmitoleic acid 1 h apart (FAEE-AP) or 12 hourly i.p. injections of caerulein (CER-AP). In all treatment groups, I-BET-762 (30 mg/kg, i...
June 10, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#15
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in S. cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28625740/comparative-proteomic-analysis-of-two-transgenic-low-gliadin-wheat-lines-and-non-transgenic-wheat-control
#16
María Dolores García-Molina, Vera Muccilli, Rosaria Saletti, Salvatore Foti, Stefania Masci, Francisco Barro
Gluten proteins are major determinants of the bread making quality of wheat, but also of important wheat-related disorders, including coeliac disease (CD), and allergies. We carried out a proteomic study using the total grain proteins from two low-gliadin wheat lines, obtained by RNAi, and the untransformed wild type as reference. The impact of silencing on both target and on non-target proteins was evaluated. Because of the great protein complexity, we performed separate analyses of four kernel protein fractions: gliadins and glutenin subunits, and metabolic and CM-like proteins, by using a classical 2D electrophoresis gel based approach followed by RP-HPLC/nESI-MS/MS...
June 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28620410/transcriptome-analysis-of-differentially-expressed-genes-induced-by-low-and-high-potassium-levels-provides-insight-into-fruit-sugar-metabolism-of-pear
#17
Changwei Shen, Jie Wang, Xiaoqian Shi, Yalong Kang, Changyan Xie, Lirun Peng, Caixia Dong, Qirong Shen, Yangchun Xu
Potassium (K) deficiency is a common abiotic stress that can inhibit the growth of fruit and thus reduce crop yields. Little research has been conducted on pear transcriptional changes under low and high K conditions. Here, we performed an experiment with 7-year-old pot-grown "Huangguan" pear trees treated with low, Control or high K levels (0, 0.4, or 0.8 g·K2O/kg soil, respectively) during fruit enlargement and mature stages. We identified 36,444 transcripts from leaves and fruit using transcriptome sequencing technology...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28619052/heterologous-expression-of-an-%C3%AE-amylase-inhibitor-from-common-bean-phaseolus-vulgaris-in-kluyveromyces-lactis-and-saccharomyces-cerevisiae
#18
Stephanie Brain-Isasi, Alejandro Álvarez-Lueje, Thomas Joseph V Higgins
BACKGROUND: Phaseolamin or α-amylase inhibitor 1 (αAI) is a glycoprotein from common beans (Phaseolus vulgaris L.) that inhibits some insect and mammalian α-amylases. Several clinical studies support the beneficial use of bean αAI for control of diabetes and obesity. Commercial extracts of P. vulgaris are available but their efficacy is still under question, mainly because some of these extracts contain antinutritional impurities naturally present in bean seeds and also exhibit a lower specific activity αAI...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28602738/immune-response-of-olive-flounder-paralichthys-olivaceus-infected-with-the-myxosporean-parasite-kudoa-septempunctata
#19
Yeoung-Hwan Jang, Dharaneedharan Subramanian, Seung-Hwan Won, Moon-Soo Heo
This study evaluated the pathophysiological, biochemical, and immunological status of olive flounder (Paralichthys olivaceus) infected with the myxosporean parasite Kudoa septempunctata. Flounder fish collected from Kudoa-infected and uninfected farms were confirmed by microscopic and TaqMan probe-based quantitative PCR screening. Morphological, biochemical, histological, and immune gene expression analyses were performed on uninfected and infected hosts to assess the effect of K. septempunctata. Histological studies confirmed the presence of Kudoa myxospores in the trunk muscles of infected flounder fish...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28600345/transcription-factor-areb2-is-involved-in-soluble-sugar-accumulation-by-activating-sugar-transporter-and-amylase-genes
#20
Qi-Jun Ma, Mei-Hong Sun, Jing Lu, Ya-Jing Liu, Da-Gang Hu, Yu-Jin Hao
Sugars play important roles in plant growth and development, crop yield and quality as well as responses to abiotic stresses. ABA is a multi-functional hormone. However, the exact mechanism by which ABA regulates sugar accumulation is largely unknown in plants. Here, we tested the expression profile of several sugar transporter and amylase genes in response to ABA treatment. MdSUT2 and MdAREB2 were isolated and genetically transformed into apples to investigate their roles in ABA-induced sugar accumulation...
June 9, 2017: Plant Physiology
keyword
keyword
43712
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"