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https://www.readbyqxmd.com/read/27924363/characteristics-and-applications-of-recombinant-thermostable-amylopullulanase-of-geobacillus-thermoleovorans-secreted-by-pichia-pastoris
#1
M Nisha, T Satyanarayana
The 3'-deleted amylopullulanase gene from the extreme thermophile Geobacillus thermoleovorans (Gt-apuΔC) was expressed extracellularly in Pichia pastoris under both methanol-inducible AOX1 and constitutive GAP promoters. The expression of the gene (Gt-apuΔC) was higher under GAP promoter (36.2 U ml(-1), α-amylase; 33.5 U ml(-1), pullulanase) than that under AOX1 promoter (32.5 and 28.6 U ml(-1)). The heavily glycosylated Gt-apuΔC from the recombinant P. pastoris displays higher substrate specificity, thermal stability and starch saccharification efficiency than that expressed in Escherichia coli...
December 6, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27913887/transcriptomics-analysis-of-hulless-barley-during-grain-development-with-a-focus-on-starch-biosynthesis
#2
Yawei Tang, Xingquan Zeng, Yulin Wang, Lijun Bai, Qijun Xu, Zexiu Wei, Hongjun Yuan, Tashi Nyima
Hulless barley, with its unique nutritional value and potential health benefits, has increasingly attracted attentions in recent years. However, the transcription dynamics during hulless barley grain development is not well understood. In the present study, we investigated the transcriptome changes during barley grain development using Illumina paired-end RNA-sequencing. Two datasets of the developing grain transcriptomes from two barley landraces with the differential seed starch synthesis traits were generated, and comparative transcriptome approach in both genotypes was performed...
December 2, 2016: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27880961/characterization-of-aspergillus-sojae-isolated-from-meju-korean-traditional-fermented-soybean-bricks
#3
Kyung Min Kim, Jaeho Lim, Jae Jung Lee, Byung-Serk Hurh, Inhyung Lee
Initially, we screened 18 A. sojae-like strains from Aspergillus spp. isolated from meju (Korean traditional fermented soybean bricks) according to their morphological characteristics. Because members of Aspergillus section Flavi are often incorrectly identified because of their phylogenetic similarity, we re-identified these strains at morphological and molecular genetic levels. Fourteen strains were finally identified as A. sojae. The isolates produced protease and α-amylase with ranges of 2.66-10.64 and 21...
November 23, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27866313/two-myb-related-transcription-factors-play-opposite-roles-in-sugar-signaling-in-arabidopsis
#4
Yi-Shih Chen, Yi-Chi Chao, Tzu-Wei Tseng, Chun-Kai Huang, Pei-Ching Lo, Chung-An Lu
Sugar regulation of gene expression has profound effects at all stages of the plant life cycle. Although regulation at the transcriptional level is one of the most prominent mechanisms by which gene expression is regulated, only a few transcription factors have been identified and demonstrated to be involved in the regulation of sugar-regulated gene expression. OsMYBS1, an R1/2-type MYB transcription factor, has been demonstrated to be involved in sugar- and hormone-regulated α-amylase gene expression in rice...
November 19, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/27864852/h-pyrophosphatase-ibvp1-promotes-efficient-iron-use-in-sweet-potato-ipomoea-batatas-l-lam
#5
Weijuan Fan, Hongxia Wang, Yinliang Wu, Nan Yang, Jun Yang, Peng Zhang
Iron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions because of decreased availability of iron in alkaline soils. Sweet potato [Ipomoea batatas (L.) Lam.] grows well in arid regions and is tolerant to Fe deficiency. Here we report that the transcription of type I H(+) -pyrophosphatase (H(+) -PPase) gene IbVP1 in sweet potato plants was strongly induced by Fe deficiency and auxin in hydroponics, improving Fe acquisition via increased rhizosphere acidification and auxin regulation...
November 18, 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/27864513/construction-of-a-male-sterility-system-for-hybrid-rice-breeding-and-seed-production-using-a-nuclear-male-sterility-gene
#6
Zhenyi Chang, Zhufeng Chen, Na Wang, Gang Xie, Jiawei Lu, Wei Yan, Junli Zhou, Xiaoyan Tang, Xing Wang Deng
The breeding and large-scale adoption of hybrid seeds is an important achievement in agriculture. Rice hybrid seed production uses cytoplasmic male sterile lines or photoperiod/thermo-sensitive genic male sterile lines (PTGMS) as female parent. Cytoplasmic male sterile lines are propagated via cross-pollination by corresponding maintainer lines, whereas PTGMS lines are propagated via self-pollination under environmental conditions restoring male fertility. Despite huge successes, both systems have their intrinsic drawbacks...
December 6, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27853181/selective-sweep-on-human-amylase-genes-postdates-the-split-with-neanderthals
#7
Charlotte E Inchley, Cynthia D A Larbey, Nzar A A Shwan, Luca Pagani, Lauri Saag, Tiago Antão, Guy Jacobs, Georgi Hudjashov, Ene Metspalu, Mario Mitt, Christina A Eichstaedt, Boris Malyarchuk, Miroslava Derenko, Joseph Wee, Syafiq Abdullah, François-Xavier Ricaut, Maru Mormina, Reedik Mägi, Richard Villems, Mait Metspalu, Martin K Jones, John A L Armour, Toomas Kivisild
Humans have more copies of amylase genes than other primates. It is still poorly understood, however, when the copy number expansion occurred and whether its spread was enhanced by selection. Here we assess amylase copy numbers in a global sample of 480 high coverage genomes and find that regions flanking the amylase locus show notable depression of genetic diversity both in African and non-African populations. Analysis of genetic variation in these regions supports the model of an early selective sweep in the human lineage after the split of humans from Neanderthals which led to the fixation of multiple copies of AMY1 in place of a single copy...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27847555/sulforaphane-protects-pancreatic-acinar-cell-injury-by-modulating-nrf2-mediated-oxidative-stress-and-nlrp3-inflammatory-pathway
#8
Zhaojun Dong, Haixiao Shang, Yong Q Chen, Li-Long Pan, Madhav Bhatia, Jia Sun
Acute pancreatitis (AP) is characterized by early activation of intra-acinar proteases followed by acinar cell death and inflammation. Cellular oxidative stress is a key mechanism underlying these pathological events. Sulforaphane (SFN) is a natural organosulfur antioxidant with undescribed effects on AP. Here we investigated modulatory effects of SFN on cellular oxidation and inflammation in AP. AP was induced by cerulean hyperstimulation in BALB/c mice. Treatment group received a single dose of 5 mg/kg SFN for 3 consecutive days before AP...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27836720/effect-of-dietary-poly-%C3%AE-hydroxybutyrate-phb-on-growth-performance-intestinal-health-status-and-body-composition-of-pacific-white-shrimp-litopenaeus-vannamei-boone-1931
#9
Yafei Duan, Yue Zhang, Hongbiao Dong, Xiaoting Zheng, Yun Wang, Hua Li, Qingsong Liu, Jiasong Zhang
In the present study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on the growth performance, intestinal digestive and immune function, intestinal short-chain fatty acids (SCFA) content and body composition of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) was evaluated. The shrimp was fed for 35 days with four different diets: 0%, 1%, 3% and 5% PHB supplemented feed. The results indicated that supplementation of PHB significantly increased the growth performance of the shrimp, and the feed conversion rate (FCR) in 3%PHB treatment group was significantly lower than the control (P < 0...
November 8, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27836003/adult-human-pancreas-derived-cells-expressing-stage-specific-embryonic-antigen-4-differentiate-into-sox9-expressing-and-ngn3-expressing-pancreatic-ducts-in-vivo
#10
Song Lee, Chan Mi Lee, Song Cheol Kim
BACKGROUND: Tissue-specific stem/progenitor cells are found in various adult tissues and may have the capacity for lineage-specific differentiation, facilitating applications in autologous transplantation. Stage-specific embryonic antigen 4 (SSEA-4), an early embryonic glycolipid antigen, is expressed in cells derived from adult human pancreas exocrine tissue. Here, we examined the characteristics and lineage-specific differentiation capacity of SSEA-4(+) cells. METHODS: Human adult partial pancreas tissues were obtained from different donors and cultured in vitro...
November 11, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27828598/lipoprotein-lipase-gene-deficient-mice-with-hypertriglyceridaemia-associated-with-acute-pancreatitis
#11
Maochun Tang, Pengfei Zong, Ting Zhang, Dongyan Wang, Yuhui Wang, Yan Zhao
PURPOSE: To investigate the severity of pancreatitis in lipoprotein lipase (LPL)-deficient hypertriglyceridaemic (HTG) heterozygous mice and to establish an experimental animal model for HTG pancreatitis study. METHODS: LPL-deficient HTG heterozygous mice were rescued by somatic gene transfer and mated with wild-type mice. The plasma amylase, triglyceride, and pathologic changes in the pancreas of the LPL-deficient HTG heterozygous mice were compared with those of wild-type mice to assess the severity of pancreatitis...
October 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27825487/phytohormone-production-endowed-with-antagonistic-potential-and-plant-growth-promoting-abilities-of-culturable-endophytic-bacteria-isolated-from-clerodendrum-colebrookianum-walp
#12
Ajit Kumar Passari, Vineet Kumar Mishra, Vincent Vineeth Leo, Vijai Kumar Gupta, Bhim Pratap Singh
In this study, culturable endophytic bacterial isolates obtained from an ethnomedicinal plant Clerodendrum colebrookianum Walp., were assessed for their diversity, in vitro screening for their plant growth promoting (PGP) activities and to use them as inoculant for in vivo PGP activities with biocontrol potential. Totally, 73 isolates were recovered from different tissues of C. colebrookianum were identified by 16S rRNA gene sequencing and phylogenetically analyzed by using BOX-PCR fingerprinting. Out of 73 isolates, 52 exhibited varying extents of antagonistic potential were selected for screening for various PGP traits...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27796570/regulating-unfolded-protein-response-activator-hac1p-for-production-of-thermostable-raw-starch-hydrolyzing-%C3%AE-amylase-in-pichia-pastoris
#13
Mengmeng Huang, Yanyun Gao, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai
Unfolded protein response (UPR) usually happens when expressing heterologous proteins in high level, which may help cells to facilitate protein processing. Here, we evaluated the effects of the UPR activator HAC1p on a raw-starch hydrolyzing α-amylase (Gs4j-amyA), so as to improve heterologous production of the enzyme in Pichia pastoris. The gene (amyA) encoding Gs4j-amyA was first codon-optimized and expressed in P. pastoris under the control of the AOX1 promoter. A high gene dosage (12 copies) of amyA facilitated amylase expression which produced an enzyme activity of 305 U/ml...
October 28, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27790066/taxonomic-characterization-evaluation-of-toxigenicity-and-saccharification-capability-of-aspergillus-section-flavi-isolates-from-korean-traditional-wheat-based-fermentation-starter-nuruk
#14
Jyotiranjan Bal, Suk-Hyun Yun, Jeesun Chun, Beom-Tae Kim, Dae-Hyuk Kim
The most economically important species used in a wide range of fermentation industries throughout Asia belong to Aspergillus section Flavi, which are morphologically and phylogenetically indistinguishable, with a few being toxigenic and therefore a major concern. They are frequently isolated from Korean fermentation starters, such as nuruk and meju. The growing popularity of traditional Korean alcoholic beverages has led to a demand for their quality enhancement, therefore requiring selection of efficient non-toxigenic strains to assist effective fermentation...
September 2016: Mycobiology
https://www.readbyqxmd.com/read/27752792/molecular-cloning-and-characterization-of-%C3%AE-amylase-subtilisin-inhibitor-from-rhizome-of-ligusticum-chuanxiong
#15
Ji-Hua Yu, Yang-Yang Li, Mian Xiang, Jian-Quan Zhu, Xin-He Huang, Wan-Jun Wang, Rui Tan, Jia-Yu Zhou, Hai Liao
OBJECTIVES: To clone and characterize a novel bi-functional α-amylase/subtilisin inhibitor (LASI) from the rhizome of Ligusticum chuanxiong, a traditional Chinese medicine. RESULTS: The LASI showed strong homology with members of the Kunitz trypsin inhibitor family. Its putative amino acid sequence has a 40 % identity with that of the α-amylase/subtilisin inhibitor from rice. LASI gene without signal peptide was expressed in E. coli Rosetta. After purification, the recombinant LASI protein was inhibitory against not only α-amylase from porcine pancreas, Helicoverpa armigera, Spodoptera litura and Plutella xylostella, but also subtilisin A, but not against trypsin or chymotrypsin...
October 17, 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27729944/expression-and-chromatin-structures-of-cellulolytic-enzyme-gene-regulated-by-heterochromatin-protein-1
#16
Xiujun Zhang, Yinbo Qu, Yuqi Qin
BACKGROUND: Heterochromatin protein 1 (HP1, homologue HepA in Penicillium oxalicum) binding is associated with a highly compact chromatin state accompanied by gene silencing or repression. HP1 loss leads to the derepression of gene expression. We investigated HepA roles in regulating cellulolytic enzyme gene expression, as an increasingly number of studies have suggested that cellulolytic enzyme gene expression is not only regulated by transcription factors, but is also affected by the chromatin status...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27718144/cardiomyopathy-as-presenting-sign-of-glycogenin-1-deficiency-report-of-three-cases-and-review-of-the-literature
#17
Carola Hedberg-Oldfors, Emma Glamuzina, Peter Ruygrok, Lisa J Anderson, Perry Elliott, Oliver Watkinson, Chris Occleshaw, Malcolm Abernathy, Clinton Turner, Nicola Kingston, Elaine Murphy, Anders Oldfors
We describe a new type of cardiomyopathy caused by a mutation in the glycogenin-1 gene (GYG1). Three unrelated male patients aged 34 to 52 years with cardiomyopathy and abnormal glycogen storage on endomyocardial biopsy were homozygous for the missense mutation p.Asp102His in GYG1. The mutated glycogenin-1 protein was expressed in cardiac tissue but had lost its ability to autoglucosylate as demonstrated by an in vitro assay and western blot analysis. It was therefore unable to form the primer for normal glycogen synthesis...
October 7, 2016: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/27714640/cloning-and-characterization-of-cold-adapted-%C3%AE-amylase-from-antarctic-arthrobacter-agilis
#18
Su-Mi Kim, Hyun Park, Jong-Il Choi
In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni(2+)-NTA affinity chromatography. This recombinant α-amylase exhibited optimal activity at pH 3.0 and 30 °C and was highly stable at varying temperatures (30-60 °C) and within the pH range of 4.0-8.0. Furthermore, α-amylase activity was enhanced in the presence of FeCl3 (1 mM) and β-mercaptoethanol (5 mM), while CoCl2 (1 mM), ammonium persulfate (5 mM), SDS (10 %), Triton X-100 (10 %), and urea (1 %) inhibited the enzymatic activity...
October 6, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27704744/influence-of-low-protein-diets-on-gene-expression-of-digestive-enzymes-and-hormone-secretion-in-the-gastrointestinal-tract-of-young-weaned-piglets
#19
Zhi-Mei Tian, Xian-Yong Ma, Xue-Fen Yang, Qiu-Li Fan, Yun-Xia Xiong, Yue-Qin Qiu, Li Wang, Xiao-Lu Wen, Zong-Yong Jiang
To investigate dietary protein level effects on digestive mechanisms, weaned piglets were fed for 45 d with diets containing 20%, 17%, or 14% crude protein (CP) supplemented to meet requirements for essential amino acids. This article describes the influence of dietary protein on gastrointestinal hormones and expression of an array of digestive enzymes in the gastrointestinal tract and pancreas. Results indicated that there were no significant differences in expression of enzymes involved in carbohydrate digestion, except for maltase in the duodenum...
2016: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/27664436/efficient-secretory-expression-of-recombinant-proteins-in-escherichia-coli-with-a-novel-actinomycete-signal-peptide
#20
Yanbing Cui, Yiwei Meng, Juan Zhang, Bin Cheng, Huijia Yin, Chao Gao, Ping Xu, Chunyu Yang
In well-established heterologous hosts, such as Escherichia coli, recombinant proteins are usually intracellular and frequently found as inclusion bodies-especially proteins possessing high rare codon content. In this study, successful secretory expression of three hydrolases, in a constructed inducible or constitutive system, was achieved by fusion with a novel signal peptide (Kp-SP) from an actinomycete. The signal peptide efficiently enabled extracellular protein secretion and also contributed to the active expression of the intracellular recombinant proteins...
January 2017: Protein Expression and Purification
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