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https://www.readbyqxmd.com/read/28819794/in-vitro-selection-and-identification-of-potential-probiotics-isolated-from-the-gastrointestinal-tract-of-nile-tilapia-oreochromis-niloticus
#1
Rasha M Reda, Khaled M Selim, Hassanin M El-Sayed, M A El-Hady
Fish gut bacteria can be used as probiotics for aquaculture. The aim of this study is to screen and identify beneficial probiotic bacteria from the gut of Nile tilapia, Oreochromis niloticus. Nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, Aeromonas sobria, Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas putida, and Staphylococcus aureus. Further tests showed that such isolates can survive in the presence of high bile concentration (10%) and at different acidic pH values...
August 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28819233/biology-physiology-and-gene-expression-of-grasshopper-oedaleus-asiaticus-exposed-to-diet-stress-from-plant-secondary-compounds
#2
Xunbing Huang, Jingchuan Ma, Xinghu Qin, Xiongbing Tu, Guangchun Cao, Guangjun Wang, Xiangqun Nong, Zehua Zhang
We studied the role of plant primary and secondary metabolites in mediating plant-insect interactions by conducting a no-choice single-plant species field experiment to compare the suitability, enzyme activities, and gene expression of Oedaleus asiaticus grasshoppers feeding on four host and non-host plants with different chemical traits. O. asiaticus growth showed a positive relationship to food nutrition content and a negative relationship to secondary compounds content. Grasshopper amylase, chymotrypsin, and lipase activities were positively related to food starch, crude protein, and lipid content, respectively...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811584/nanopriming-technology-for-enhancing-germination-and-starch-metabolism-of-aged-rice-seeds-using-phytosynthesized-silver-nanoparticles
#3
Wuttipong Mahakham, Ajit K Sarmah, Santi Maensiri, Piyada Theerakulpisut
Application of nanomaterials for agriculture is relatively new as compared to their use in biomedical and industrial sectors. In order to promote sustainable nanoagriculture, biocompatible silver nanoparticles (AgNPs) have been synthesized through green route using kaffir lime leaf extract for use as nanopriming agent for enhancing seed germination of rice aged seeds. Results of various characterization techniques showed the successful formation of AgNPs which were capped with phytochemicals present in the plant extract...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811162/engineering-peptidoglycan-degradation-related-genes-of-bacillus-subtilis-for-better-fermentation-processes
#4
Liang Zhao, Jianwen Ye, Jing Fu, Guo-Qiang Chen
In this study, Bacillus subtilis 168 Δupp was engineered to change the bacterial shapes. Namely, some peptidoglycan hydrolase related genes were inactivated individually or in different combinations, including sigD, lytE, lytF, lytC, lytD and lytG. Inactivations of these genes resulted in various intensities of blockages on cell division, leading to elongation of bacterial cells. The resulted fiber phenotypes showed different lengths ranging from tens of microns to several millimeters. Mutants with multiple gene inactivations such as ΔsigDΔlytEΔlytD showed more easily precipitated phenomenon, obviously increased growth rate, more sensitive to antibiotics and improved α-amylase production compared with that of B...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28804584/the-expression-of-activating-receptor-gene-of-natural-killer-cells-klrc3-in-patients-with-%C3%A2-type-1-diabetes-mellitus-t1dm
#5
Dalia Shalaby, Marwa Saied, Doaa Khater, Abla Abou Zeid
OBJECTIVES: To identify the possible role of natural killer (NK) cells in the pathogenesis of type 1 diabetes mellitus (T1DM) through studying the expression of the KLRC3 gene, which encodes the NK cell activating receptor (NKG2E). METHODS: This study was conducted at Alexandria University Children's Hospital from April to October 2015. The study was conducted with 30 newly diagnosed T1DM patients (15 males and 15 females), aged 7-13 years (10.6±1.8 years) and 20 non-diabetic subjects served as age- and sex-matched controls...
July 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/28783453/development-of-human-salivary-gland-like-tissue-in-vitro
#6
Marc Burghartz, Simon Lennartz, Matthias Schweinlin, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg, Gudrun Steußloff, Agmal Scherzad, Kathrin Radeloff, Christian Ginzkey, Heike Walles, Marco Metzger
The loss of salivary gland function caused by radiation therapy of the head and neck is a serious condition and it affects a patient's quality of life. The current lack of effective therapies demands new options to be explored. This study tested whether human salivary gland epithelial cells (SGECs) could be successfully cultured on a decellularized porcine gut matrix (SIS-muc) in both mono- and coculture with microvascular endothelial cells (mvECs). By performing immunofluorescence imaging, transmission as well as scanning electron microscopy (SEM), quantitative polymerase chain reaction (qPCR), and an amylase enzyme assay, it was investigated as to what extent the three-dimensional (3D)-cultured cells could maintain their molecular differentiation and the production of working α-amylase (α-AMY) compared with two-dimensional (2D) culture...
August 4, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28729759/transcriptome-wide-identification-and-characterization-of-starch-branching-enzyme-in-finger-millet
#7
Rajhans Tyagi, Apoorv Tiwari, Vijay Kumar Garg, Sanjay Gupta
Starch-branching enzymes (SBEs) are one of the four major enzyme classes involved in starch biosynthesis in plants and play an important role in determining the structure and physical properties of starch granules. Multiple SBEs are involved in starch biosynthesis in plants. Finger millet is calcium rich important serial crop belongs to grass family and the transcriptome data of developing spikes is available on NCBI. In this study it was try to find out the gene sequence of starch branching enzyme and annotate the sequence and submit the sequence for further use...
2017: Bioinformation
https://www.readbyqxmd.com/read/28727805/turgor-responsive-starch-phosphorylation-in-oryza-sativa-stems-a-primary-event-of-starch-degradation-associated-with-grain-filling-ability
#8
Hiroshi Wada, Chisato Masumoto-Kubo, Koichi Tsutsumi, Hiroshi Nonami, Fukuyo Tanaka, Haruka Okada, Rosa Erra-Balsells, Kenzo Hiraoka, Taiken Nakashima, Makoto Hakata, Satoshi Morita
Grain filling ability is mainly affected by the translocation of carbohydrates generated from temporarily stored stem starch in most field crops including rice (Oryza sativa L.). The partitioning of non-structural stem carbohydrates has been recognized as an important trait for raising the yield ceiling, yet we still do not fully understand how carbohydrate partitioning occurs in the stems. In this study, two rice subspecies that exhibit different patterns of non-structural stem carbohydrates partitioning, a japonica-dominant cultivar, Momiroman, and an indica-dominant cultivar, Hokuriku 193, were used as the model system to study the relationship between turgor pressure and metabolic regulation of non-structural stem carbohydrates, by combining the water status measurement with gene expression analysis and a dynamic prefixed 13C tracer analysis using a mass spectrometer...
2017: PloS One
https://www.readbyqxmd.com/read/28725557/genome-assembly-of-chryseobacterium-sp-strain-ihbb-10212-from-glacier-top-surface-soil-in-the-indian-trans-himalayas-with-potential-for-hydrolytic-enzymes
#9
Mohinder Pal, Mohit Kumar Swarnkar, Hena Dhar, Sanjay Chhibber, Arvind Gulati
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas...
September 2017: Genomics Data
https://www.readbyqxmd.com/read/28725229/the-synergistic-priming-effect-of-exogenous-salicylic-acid-and-h2o2-on-chilling-tolerance-enhancement-during-maize-zea-mays-l-seed-germination
#10
Zhan Li, Jungui Xu, Yue Gao, Chun Wang, Genyuan Guo, Ying Luo, Yutao Huang, Weimin Hu, Mohamed S Sheteiwy, Yajing Guan, Jin Hu
Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H2O2) in response to chilling stress, we investigated the effects of seed priming with SA, H2O2, and SA+H2O2 combination on maize resistance under chilling stress (13°C). Priming with SA, H2O2, and especially SA+H2O2 shortened seed germination time and enhanced seed vigor and seedling growth as compared with hydropriming and non-priming treatments under low temperature...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28714845/bacillus-mangrovi-sp-nov-isolated-from-a-sediment-sample-from-a-mangrove-forest
#11
Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities...
July 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28709869/protective-effect-of-bacillopeptidase-cfr5-from-bacillus-subtilis-cfr5-on-cerulein-induced-pancreatitis
#12
G R Sharmila, G Venkateswaran
Bacillopeptidase is a serine peptidase, known for its fibrinolytic activity. However, a very little information is known about its in vivo inflammatory and/or anti-inflammatory properties. Thus, to understand whether bacillopeptidase incorporation can regulate pancreatitis or not, the cerulein-induced pancreatitis model was used, and the role of bacillopeptidase on pancreatitis was studied. In this study, 46 kDa protein was purified from Bacillus subtilis and identified as bacillopeptidase CFR5 (BPC) through MS/MS analysis...
July 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28684177/improvement-of-cellulolytic-enzyme-production-and-performance-by-rational-designing-expression-regulatory-network-and-enzyme-system-composition
#13
REVIEW
Zhonghai Li, Guodong Liu, Yinbo Qu
Filamentous fungi are considered as the most efficient producers expressing lignocellulose-degrading enzymes. Penicillium oxalicum strains possess extraordinary fungal lignocellulolytic enzyme systems and can efficiently utilize plant biomass. In recent years, the regulatory aspects of production of hydrolytic enzymes by P. oxalicum have been well established. This review aims to discuss the recent developments for the production of lignocellulolytic enzymes by P. oxalicum. The main cellulolytic transcription factors mediating the complex transcriptional-regulatory network are highlighted...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28683528/isolation-and-characterization-of-some-promoter-sequences-from-leuconostoc-mesenteroides-sy2-isolated-from-kimchi
#14
Ji Yeong Park, Seon-Ju Jeong, Jeong A Kim, Jeong Hwan Kim
Some promoters were isolated and characterized from genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI cut pBV5030, a promoter screening vector containing a promoter-less cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for 3 putative promoters (P04C, P25C, and P33C) by primer extension...
July 7, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28678349/construction-of-a-multi-control-sterility-system-for-a-maize-male-sterile-line-and-hybrid-seed-production-based-on-the-zmms7-gene-encoding-a-phd-finger-transcription-factor
#15
Danfeng Zhang, Suowei Wu, Xueli An, Ke Xie, Yan Zhou, Liwen Xu, Wen Fang, Shensi Liu, Shuangshuang Liu, Taotao Zhu, Jinping Li, Liqun Rao, Jiuran Zhao, Xiangyuan Wan
Although hundreds of genetic male sterility (GMS) mutants have been identified in maize, few are commercially used due to a lack of effective methods to produce large quantities of pure male-sterile seeds. Here, we develop a multi-control sterility (MCS) system based on the maize male sterility 7 (ms7) mutant and its wild-type Zea mays Male sterility 7 (ZmMs7) gene via a transgenic strategy, leading to the utilization of GMS in hybrid seed production. ZmMs7 is isolated by a map-based cloning approach, and encodes a PHD-finger transcription factor orthologous to rice PTC1 and Arabidopsis MS1...
July 5, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28662104/vibrio-aphrogenes-sp-nov-in-the-rumoiensis-clade-isolated-from-a-seaweed
#16
Mami Tanaka, Shoko Endo, Fumihito Kotake, Nurhidayu Al-Saari, A K M Rohul Amin, Gao Feng, Sayaka Mino, Hidetaka Doi, Yoshitoshi Ogura, Tetsuya Hayashi, Wataru Suda, Masahira Hattori, Isao Yumoto, Toko Sawabe, Tomoo Sawabe, Toshiyoshi Araki
A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio...
2017: PloS One
https://www.readbyqxmd.com/read/28659346/starch-digestion-related-amylase-genetic-variant-affects-2-year-changes-in-adiposity-in-response-to-weight-loss-diets-the-pounds-lost-trial
#17
Yoriko Heianza, Dianjianyi Sun, Tiange Wang, Tao Huang, George A Bray, Frank M Sacks, Lu Qi
Salivary and pancreatic amylases (encoded by AMY1 and AMY2 genes, respectively) are responsible for digesting starchy foods. AMY1 and AMY2 show copy number variations which affect differences in amylase amount and activity, and it is suggested that AMY1 copies are associated with adiposity. We investigated whether genetic variants determining amylase gene copies were associated with 2-year changes of adiposity among 692 overweight and obese individuals who were randomly assigned to diets varying in macronutrient contents...
June 28, 2017: Diabetes
https://www.readbyqxmd.com/read/28655353/xc_0531-encodes-a-c-type-cytochrome-biogenesis-protein-and-is-required-for-pathogenesis-in-xanthomonas-campestris-pv-campestris
#18
Lei Chen, Mingpeng Wang, Li Huang, Zhaojie Zhang, Fanghua Liu, Guangtao Lu
BACKGROUND: The phytopathogenic Xanthomonas campestris pv.campestris is a gram-negative bacterium and the causal agent of black-rot disease of cruciferous crops. Many gram-negative bacteria possess a family of proteins, called Dsbs, which are involved in disulfide bond formation in certain periplasmic proteins. In our preliminary screening of the virulence to the plants we identified that gene XC_0531 which annotated gene dsbD of Xanthomonas campestris pv. campestris (Xcc) is related to the virulence to the host plants...
June 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28648523/functional-characterization-and-crystal-structure-of-thermostable-amylase-from-thermotoga-petrophila-reveals-high-thermostability-and-an-unusual-form-of-dimerization
#19
Uzma Hameed, Ian Price, Ikram-Ul-Haq, Ailong Ke, David B Wilson, Osman Mirza
Thermostable α-amylases have many industrial applications and are therefore continuously explored from novel sources. We present the characterization of a novel putative α-amylase gene product (Tp-AmyS) cloned from Thermotoga petrophila. The purified recombinant enzyme is highly thermostable and able to hydrolyze starch into dextrin between 90 and 100°C, with optimum activity at 98°C and pH8.5. The activity increased in the presence of Rb(1+), K(1+) and Ca(2+) ions, whereas other ions inhibited activity...
June 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28648518/selective-inhibition-of-bet-proteins-reduces-pancreatic-damage-and-systemic-inflammation-in-bile-acid-and-fatty-acid-ethyl-ester-but-not-caerulein-induced-acute-pancreatitis-in-mice
#20
Wei Huang, Andrea C Haynes, Rajarshi Mukherjee, Li Wen, Diane Latawiec, Alexei V Tepikin, David N Criddle, Rab K Prinjha, Nicholas Smithers, Robert Sutton
OBJECTIVES: To evaluate the therapeutic potential of I-BET-762, an inhibitor of the bromodomain and extra-terminal (BET) protein family, in experimental acute pancreatitis (AP). METHODS: AP was induced in mice by retrograde infusion of taurolithocholic acid sulphate into the biliopancreatic duct (TLCS-AP) or two intraperitoneal (i.p.) injections of ethanol and palmitoleic acid 1 h apart (FAEE-AP) or 12 hourly i.p. injections of caerulein (CER-AP). In all treatment groups, I-BET-762 (30 mg/kg, i...
June 10, 2017: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
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