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gene amylase

Arzu Coleri Cihan, Emine Derebay Yildiz, Ergin Sahin, Ozal Mutlu
Among the thermophilic Bacillaceae family members, α-amylase production of 15 bacilli from genus Anoxybacillus was investigated, some of which are biotechnologically important. These Anoxybacillus α-amylase genes displayed ≥ 91.0% sequence similarities to Anoxybacillus enzymes (ASKA, ADTA and GSX-BL), but relatively lower similarities to Geobacillus (≤ 69.4% to GTA, Gt-amyII), and Bacillus aquimaris (≤ 61.3% to BaqA) amylases, all formerly proposed only in a Glycoside Hydrolase 13 (GH13) subfamily...
June 15, 2018: World Journal of Microbiology & Biotechnology
Poulomi Saha, Mohd Faheem Khan, Sanjukta Patra
Enzymes are indispensable biocatalysts required in various steps of textile processing to minimize various chemical-induced hazards. The present work focuses on the applications of the truncated α-amylase in textile industry for desizing of fabrics by starch hydrolysis. The multiple sequence alignment was performed to find homology and the possible truncation region in Bacillus subtilis MTCC 121 α-amylase with same bacilli family α-amylase. Two constructs were generated for α-amylase gene of Bacillus subtilis MTCC 121 (Amy_F, full-length and Amy_T, C-terminal truncated) were cloned, overexpressed, purified, and characterized...
June 14, 2018: Preparative Biochemistry & Biotechnology
José R Valverde, Sonia Gullón, Rafael P Mellado
BACKGROUND: Streptomyces lividans has demonstrated its value as an efficient host for protein production due to its ability to secrete functional proteins directly to the media. Secretory proteins that use the major Sec route need to be properly folded outside the cell, whereas secretory proteins using the Tat route appear outside the cell correctly folded. This feature makes the Tat system very attractive for the production of natural or engineered Tat secretory proteins. S. lividans cells are known to respond differently to overproduction and secretion of Tat versus Sec proteins...
June 14, 2018: BMC Microbiology
Seung Hoon Jang, Ji Won Cha, Nam Soo Han, Ki Jun Jeong
The lactic acid bacteria (LAB) Leuconostoc citreum are non-sporulating hetero-fermentative bacteria that play an important role in the fermented food industry. In this study, for the enhanced and reliable production of recombinant proteins in L. citreum, we developed a bicistronic design (BCD) expression system which includes a short leader peptide (1st cistron) followed by target genes (2nd cistron) under the control of a single promoter. Using superfolder green fluorescent protein (sfGFP) as a reporter, the functionality of BCD in L...
June 11, 2018: Scientific Reports
Irana Paim Silva, Camila de Souza Carneiro, Margarete Alice Fontes Saraiva, Thiago Alves Santos de Oliveira, Oscarina Viana de Sousa, Norma Suely Evangelista-Barreto
The aim of the present study was to verify the antimicrobial susceptibility profile and virulence factors of Vibrio parahaemolyticus isolated from water and bivalve mollusks. A high percentage of V. parahaemolyticus was isolated in natura, processed bivalves tissues, and surrounding water (75%, 20%, and 59%, respectively). The most potential virulence phenotype in V. parahaemolyticus isolates was amylase production (97%) followed by DNase (83%), phospholipase (70%), β-hemolytic activity (57%). The tdh and trh genes were not detected...
June 2018: Marine Pollution Bulletin
Oghenetega J Avwioroko, Akpovwehwee A Anigboro, Nnanna N Unachukwu, Nyerhovwo J Tonukari
In this investigation, a gene ( CDF_Amyl ) encoding extracellular α-amylase in Aspergillus niger strain CSA35 associated with cassava spoilage was amplified using specific primers and characterized in silico . The gene had a partial nucleotide sequence of 968 bp and encoded a protein of 222 aa residues with a molecular weight and isoelectric point of 25.13 kDa and 4.17, respectively. Its catalytic site was located in the active site domain. BLASTp analysis showed that the protein primary sequence of the α-amylase gene had 98% and 99% homologies with the α-amylase of A...
July 2018: Biochemistry and Biophysics Reports
Guang Yang, Yule Hu, Opemipo E Fasoyin, Yuewei Yue, Lijie Chen, Yue Qiu, Xiuna Wang, Zhenhong Zhuang, Shihua Wang
Reversible protein phosphorylation is known to play important roles in the regulation of various cellular processes in eukaryotes. Phosphatase-mediated dephosphorylation are integral components of cellular signal pathways by counteracting the phosphorylation action of kinases. In this study, we characterized the functions of CDC14, a dual-specificity phosphatase in the development, secondary metabolism and crop infection of Aspergillus flavus . Deletion of AflCDC14 resulted in a growth defect and abnormal conidium morphology...
2018: Frontiers in Cellular and Infection Microbiology
Kazumi Funane, Yukinori Tanaka, Takeshi Hosaka, Kiriko Murakami, Takatsugu Miyazaki, Yuh Shiwa, Shigehachi Gibu, Takeshi Inaoka, Ken Kasahara, Nobuyuki Fujita, Hirofumi Yoshikawa, Yoshikazu Hiraga, Kozo Ochi
This study shows that sequential introduction of drug-resistance mutations substantially increased enzyme production in Paenibacillus agaridevorans The triple mutant YT478 ( rsmG Gln225→stop codon, rpsL K56R, rpoB R485H), generated by screening for resistance to streptomycin and rifampicin, expressed a 1,100-fold higher amount of the extracellular enzyme, cycloisomaltooligosaccharide glucanotransferase (CITase), than the wild-type strain. These mutants were characterized by higher intracellular S -adenosylmethionine concentrations during exponential phase and enhanced protein synthesis activity during stationary phase...
June 4, 2018: Journal of Bacteriology
Nan-Nan Liu, Zhe Chi, Guang-Lei Liu, Tie-Jun Chen, Hong Jiang, Zhong Hu, Zhen-Ming Chi
A high molecular weight (Mw) pullulan has many potential applications in various fields. α-Amylase, glucoamylase and pullulanase were thought to play an important role in high Mw pullulan biosynthesis. However, there is no genetic evidence for this role. In this study, the genes encoding α-amylase, glucoamylase and pullulanase were cloned from Aureobasidium melanogenum P16, a high pullulan producing yeast and characterized. The proteins deduced from the cloned α-amylase gene, the glucoamylase gene and the isopullulanase gene, not a pullululanse gene had their corresponding conserved amino acid sequences, respectively...
June 1, 2018: International Journal of Biological Macromolecules
Yafei Duan, Jiasong Zhang, Jianhua Huang, Shigui Jiang
The present study investigated the effects of the dietary probiotic Clostridium butyricum (CB) on the growth, intestine digestive enzyme activity, antioxidant capacity and resistance to nitrite stress, and body composition of Penaeus monodon. For 56 days, shrimps were fed diets containing different levels of C. butyricum (1 × 109  CFU g-1 ), 0% (control), 0.5% (CB1), 1.0% (CB2), and 2.0% (CB3), as treatment groups, followed by an acute nitrite stress test for 48 h. The results indicated that dietary supplementation of C...
June 1, 2018: Probiotics and Antimicrobial Proteins
Verena Christen, Melanie Schirrmann, Juerg Frey, Karl Fent
Neonicotinoids are implicated in the decline of honey bees but the molecular basis underlying adverse effects are poorly known. Here we describe global transcriptomic profiles in the brain of honey bee workers exposed for 48 h at one environmentally realistic and one sublethal concentration of 0.3 and 3.0 ng/bee clothianidin and imidacloprid, respectively, and 0.1 and 1.0 ng/bee thiamethoxam (1-30 ng/mL sucrose solution) by high-throughput RNA-sequencing. All neonicotinoids led to significant alteration (mainly down-regulation) of gene expression, generally with a concentration-dependent effect...
May 31, 2018: Environmental Science & Technology
Guanbao Xu, Jingen Li, Qian Liu, Wenliang Sun, Min Jiang, Chaoguang Tian
Thermophilic fungus Myceliophthora thermophila has great capacity for biomass degradation and is an attractive option for use as cell factory to produce chemicals directly from renewable polysaccharides, such as starch, rather than monomer glucose. To date, there has been no transcriptomic analysis of this thermophilic fungus on starch. This study determined the transcriptomic profile of M. thermophila responding to soluble starch and a 342-gene set was identified as a "starch regulon", including the major amylolytic enzyme (Mycth_72393)...
May 24, 2018: Bioresource Technology
Ahmed A Elolimy, Sonia J Moisá, Kristen M Brennan, Allison C Smith, Daniel Graugnard, Daniel W Shike, Juan J Loor
Our main objective was to evaluate the effects of feeding α-amylase (Amaize, Alltech Inc., Nicholasville, KY, USA) for 140 days on skeletal muscle and liver gene transcription in beef steers. Steers fed Amaize had lower average daily gain (p = .03) and gain:feed ratio (p = .05). No differences (p > .10) in serum metabolites or carcass traits were detected between the two groups but Amaize steers tended (p < .15) to have increased 12th rib fat depth. Microarray analysis of skeletal muscle revealed 21 differentially expressed genes (DEG), where 14 were up-regulated and seven were down-regulated in Amaize-fed steers...
May 29, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
André C Pimentel, Ignacio G Barroso, Jéssica M J Ferreira, Renata O Dias, Clélia Ferreira, Walter R Terra
Until now there is no molecular model of starch digestion and absorption of the resulting glucose molecules along the larval midgut of Musca domestica. For addressing to this, we used RNA-seq analyses from seven sections of the midgut and carcass to evaluate the expression level of the genes coding for amylases, maltases and sugar transporters (SP). An amylase related protein (Amyrel) and two amylase sequences, one soluble and one with a predicted GPI-anchor, were identified. Three highly expressed maltase genes were correlated with biochemically characterized maltases: one soluble, other glycocalyx-associated, and another membrane-bound...
May 24, 2018: Journal of Insect Physiology
Ahmad Khosravi, Iraj Sharifi, Hadi Tavakkoli, Amin Derakhshanfar, Ali Reza Keyhani, Zohreh Salari, Seyedeh Saedeh Mosallanejad, Mehdi Bamorovat
Leishmaniasis is one of the diverse and neglected tropical diseases. Embryo-toxicity of drugs has always been a major concern. Chick embryo is a preclinical model relevant in the assessment of adverse effects of drugs. The current study aimed to assess embryonic histopathological disorders and amniotic fluid biochemical changes following meglumine antimoniate treatment. The alteration of vascular branching pattern in the chick's extra-embryonic membrane and exploration of molecular cues to early embryonic vasculogenesis and angiogenesis were also quantified...
2018: PloS One
Daniel Pereira de Paiva, Tiago Benoliel Rocha, Marciano Regis Rubini, André Moraes Nicola, Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Lidia Maria Pepe de Moraes
Polymorphism is well known in Saccharomyces cerevisiae strains used for different industrial applications, however little is known about its effects on promoter efficiency. In order to test this, five different promoters derived from an industrial and a laboratory (S288c) strain were used to drive the expression of eGFP reporter gene in both cells. The ADH1 promoter (P ADH1 ) in particular, which showed more polymorphism among the promoters analyzed, also exhibited the highest differences in intracellular fluorescence production...
May 21, 2018: AMB Express
Jie Zhou, Zhoukun Li, Han Zhang, Jiale Wu, Xianfeng Ye, Weiliang Dong, Min Jiang, Yan Huang, Zhongli Cui
Here, a novel amylolytic enzyme gene designated coMA was cloned from Corallococcus sp. EGB. The deduced amino acid sequence contained a predicted lipoprotein signal peptide (residues 1-18) and conserved glycoside hydrolase family 13 (GH13) module. The amino acid sequence of CoMA exhibits low sequence identity (10-19%) with cyclodextrin-hydrolyzing enzymes (GH13_20) and is assigned to GH13_36. The most outstanding feature of CoMA is its ability to catalyze the conversion of maltooligosaccharides (≥ G3) and soluble starch to maltose as the sole hydrolysate...
May 11, 2018: Applied and Environmental Microbiology
Conrad Wiegand, Peter Heusser, Claudia Klinger, Dirk Cysarz, Arndt Büssing, Thomas Ostermann, Andreas Savelsbergh
Stress is an important co-factor for the genesis and maintenance of many diseases and is known to have an effect on gene expression via epigenetic regulation. MicroRNAs (miRNAs) appear to function as one of the key factors of this regulation. This is the first study to investigate the response of 11 stress-associated miRNAs in human saliva - as a non-invasive source - in an experimental condition of acute psychological stress, and also their correlation with established psychological (subjective stress perception), physiological (heart rate and heart rate variability) and biochemical stress parameters (salivary cortisol and alpha-amylase)...
May 8, 2018: Scientific Reports
Ruijie Gao, Zhecun Xu, Huaxiang Deng, Zhengbing Guan, Xiangru Liao, Ye Zhao, Xiaohui Zheng, Yujie Cai
Relative expression levels of twenty-four amylase genes in Shiraia sp. SUPER-H168 were investigated by real-time quantitative PCR when various carbohydrates, including glucose, sucrose, maltose, amylose, amylopectin and corn flour, were used as carbon source. Genes, including an α-glucosidase gene Amy33 (2997 bp), an α-amylase gene Amy365-1 (1749 bp) and a glycogen debranching enzyme gene Amy130 (2487 bp), were overexpressed, and four overexpression transformants were constructed, respectively. When Amy365-1 and Amy130 were co-overexpressed, relative expression levels of seven hypocrellin biosynthesis genes and four related genes in central carbon catabolism were all increased...
2018: PloS One
Ruijie Gao, Huaxiang Deng, Zhengbing Guan, Xiangru Liao, Yujie Cai
Shiraia bambusicola is an important and valuable macrofungus and hypocrellins are its main secondary metabolites which have been widely applied in many medical fields. However, during SSF process of this filamentous fungus, use ratio of corn substrate and dissolved oxygen supply are two main limiting factors, which influence production cost, yield and product quality. To solve these problems, overexpressions of amy365-1 and vgb in S. bambusicola were investigated and three overexpression transformants were constructed...
May 2, 2018: AMB Express
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