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https://www.readbyqxmd.com/read/29449858/exogenous-hydrogen-peroxide-contributes-to-heme-oxygenase-1-delaying-programmed-cell-death-in-isolated-aleurone-layers-of-rice-subjected-to-drought-stress-in-a-cgmp-dependent-manner
#1
Guanghui Wang, Yu Xiao, Xiaojiang Deng, Heting Zhang, Tingge Li, Huiping Chen
Hydrogen peroxide (H 2 O 2 ) is a reactive oxygen species (ROS) that plays a dual role in plant cells. Here, we discovered that drought (20% polyethylene glycol-6000, PEG)-triggered decreases of HO-1 transcript expression and HO activity. However, exogenous H 2 O 2 contributed toward the increase in HO-1 gene expression and activity of the enzyme under drought stress. Meanwhile, the HO-1 inducer hematin could mimic the effects of the H 2 O 2 scavengers ascorbic acid (AsA) and dimethylthiourea (DMTU) and the H 2 O 2 synthesis inhibitor diphenyleneiodonium (DPI) for scavenging or diminishing drought-induced endogenous H 2 O 2 ...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29444820/a-modified-hypoallergenic-variant-of-the-ricinus-communis-ric-c1-protein-retains-biological-activity
#2
Thaís Pacheco-Soares, André de Oliveira Carvalho, Jucélia da Silva Araújo, Giliane da Silva de Souza, Olga L T Machado
Ric c1, an allergenic protein from Ricinus communis , is an insect α-amylase inhibitor that has become an occupational allergen. Ric c1 can cross-react with allergens from wheat, soybean, peanut, shrimp, fish, gluten, house dust, tobacco, and air fungus, thereby amplifying the concern and risks caused by Ricinus allergens. Two continuous IgE-binding epitopes were identified in Ric c1, both containing glutamic acid residues involved in IgE-binding and allergic challenges. We produced recombinant Ric c1 (rRic c1) in Escherichia coli , using primers from foliar R...
February 14, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29442225/feeder-cell-independent-culture-of-the-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line-picm-31
#3
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
The adaptation to feeder-independent growth of a pig embryonic stem cell-derived pancreatic cell line is described. The parental PICM-31 cell line, previously characterized as an exocrine pancreas cell line, was colony-cloned two times in succession resulting in the derivative cell line, PICM-31A1. PICM-31A1 cells were adapted to growth on a polymerized collagen matrix using feeder cell-conditioned medium and were designated PICM-31FF. Like the parental cells, the PICM-31FF cells were small and grew relatively slowly in closely knit colonies that eventually coalesced into a continuous monolayer...
February 13, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29439880/suppression-of-inducible-nitric-oxide-synthase-and-tumor-necrosis-factor-alpha-level-by-lycopene-is-comparable-to-methylprednisolone-in-acute-pancreatitis
#4
Nahla E El-Ashmawy, Naglaa F Khedr, Hoda A El-Bahrawy, Omnia B Hamada
BACKGROUND: Oxidative stress and inflammation may play a key role in the pathogenesis of acute pancreatitis (AP). Lycopene, a natural carotenoid, has antioxidant scavenger capacity and inhibits inflammation in many experimental models. AIM: The study was designed to investigate whether lycopene can ameliorate l-arginine-induced pancreatitis in rats and to elucidate the underlying molecular mechanisms of these effects. METHODS: Forty-eight adult male Wistar rats were divided into: control group (vehicle, orally, 10 days), AP group (3 g/kg l-arginine, single i...
February 2, 2018: Digestive and Liver Disease
https://www.readbyqxmd.com/read/29435045/the-protective-effects-of-rutaecarpine-on-acute-pancreatitis
#5
Lu Yan, Qing-Fu Li, Yan-Ting Rong, Yong-Heng Chen, Zhao-Hong Huang, Zhi-Zhi Wang, Jie Peng
Acute pancreatitis (AP) is the acute inflammation of the pancreas. The morbidity of AP has increased in recent years. Certain patients eventually develop severe AP (SAP), which rapidly progresses to multiple organ dysfunction; the incidence of this occurring in patients with AP is 20-30%. To date, no specific drugs or methods exist to treat this disease. Rutaecarpine relaxes vascular smooth muscle by stimulating calcitonin gene-related peptide (CGRP) release via activation of vanilloid receptor subtype 1 (VR1)...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29433244/identification-of-enzyme-origin-in-dough-improvers-dna-based-and-proteomic-approaches
#6
Gianluca Picariello, Luigia Di Stasio, Gianfranco Mamone, Giuseppe Iacomino, Antonella Venezia, Nunzia Iannaccone, Pasquale Ferranti, Raffaele Coppola, Francesco Addeo
Enzymatic dough improvers (DIs) are increasingly used as baking co-adjuvants. Herein, an array of techniques, including Western blotting, PCR, electrophoresis-based and shotgun proteomics, was addressed to identify the enzymes in six commercial DI preparations. In particular, this work sought to exclude the possible undeclared use of amylolytic enzymes from porcine (or other animal origin) pancreas in DIs. PCR-amplified mitochondrial cytochrome b (mt cyt b) gene region and porcine pancreatic α-amylase were the targets of DNA-based and protein methods, respectively, both assuring a limit of detection lower than 0...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29430375/complete-genome-sequence-of-bacillus-velezensis-157-isolated-from-eucommia-ulmoides-with-pathogenic-bacteria-inhibiting-and-lignocellulolytic-enzymes-production-by-ssf
#7
Long Chen, Wei Gu, Hai-Yan Xu, Gui-Lian Yang, Xiao-Feng Shan, Guang Chen, Chun-Feng Wang, Ai-Dong Qian
Bacillus velezensis 157 was isolated from the bark of Eucommia ulmoides , and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, B. velezensis 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29407618/transcriptome-analysis-of-huanghai-no-2-fenneropenaeus-chinensis-response-to-wssv-using-rna-seq
#8
REVIEW
Xiaoli Shi, Xianhong Meng, Jie Kong, Sheng Luan, Kun Luo, Baoxiang Cao, Xia Lu, Xupeng Li, Baolong Chen, Jiawang Cao
White spot syndrome (WSS) is one of the most damaging phenomena in the culturing of shrimp. To characterize the mechanisms of the molecular responses to WSSV infection in 'Huanghai No. 2'' Fenneropenaeus chinensis, we used next-generation sequencing to observe the transcriptome after oral infection. A total of 108.6 million clean reads were obtained and assembled into 64,103 final unigenes with an average length of 845 bp (N50 = 1534 bp). The assembled unigenes contained 14,263 significant unigenes after BLASTX against the Nr database (E-value cut-off of 10-5)...
January 30, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29398872/influence-of-nudt15-variants-on-hematological-pictures-of-patients-with-inflammatory-bowel-disease-treated-with-thiopurines
#9
Yuichiro Kojima, Yosuke Hirotsu, Wataru Omata, Makoto Sugimori, Shinya Takaoka, Hiroshi Ashizawa, Keiko Nakagomi, Dai Yoshimura, Kenji Hosoda, Yoji Suzuki, Hitoshi Mochizuki, Masao Omata
AIM: The single nucleotide polymorphism (SNP) c.415C>T in exon 3 of NUDT15 affects thiopurine-induced leukopenia in Asian patients with Crohn's disease. Meanwhile, three additional genetic variants of NUDT15 were reported in patients with acute lymphoblastic leukemia. We evaluated the effects of these additional genetic variants of NUDT15 in patients with inflammatory bowel disease (IBD) treated with thiopurines. METHODS: Ninety-six Japanese patients with IBD were enrolled...
January 28, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29396619/untapped-bacterial-diversity-and-metabolic-potential-within-unkeshwar-hot-springs-india
#10
Gajanan Mehetre, Manan Shah, Syed G Dastager, Mahesh S Dharne
Hot springs support diverse and interesting groups of microorganisms adapted to extreme conditions and gaining attention in biotechnological applications. However, due to limitations of cultivation methods, a majority of such extremophiles remain uncultivated and unexplored. The advent of multiple cultivation conditions and specialized culture media could possibly aid to access the unexplored microbial portion of hot springs. In the present study, different media and isolation strategies were applied to isolate hitherto unexplored bacterial taxa in the water samples collected from Unkeshwar hot springs, India...
February 2, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29393585/-metabolic-engineering-of-lactobacillus-plantarum-for-direct-l-lactic-acid-production-from-raw-corn-starch
#11
Kenji Okano, Gentaro Uematsu, Shinji Hama, Tsutomu Tanaka, Hideo Noda, Akihiko Kondo, Kohsuke Honda
Fermentative production of optically pure lactic acid (LA) has attracted great interest because of the increased demand for plant-based plastics. For cost-effective LA production, an engineered Lactobacillus plantarum NCIMB 8826 strain, which enables the production of optically pure L-LA from raw starch, was constructed. The wild-type strain produces a racemic mixture of D- and L-LA from pyruvate by the action of the respective lactate dehydrogenases (LDHs). Therefore, the gene encoding D-LDH (ldhD) was deleted...
February 2, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29393354/effects-of-silencing-the-dusp1-gene-using-lentiviral-vector-mediated-sirna-on-the-release-of-proinflammatory-cytokines-through-regulation-of-the-mapk-signaling-pathway-in-mice-with-acute-pancreatitis
#12
Bo Zhang, Shu-Liang Li, Hua-Lei Xie, Jia-Wei Fan, Chuan-Wei Gu, Chao Kang, Mu-Jian Teng
The present study investigated the effects of dual specificity phosphatase 1 (DUSP1) gene silencing using lentiviral vector-mediated small interfering (si)RNA on the release of proinflammatory cytokines through the regulation of the mitogen‑activated protein kinase (MAPK) signaling pathway in mice with acute pancreatitis (AP). Two siRNA‑DUSP1 sequences and one scramble siRNA sequence were designed, and the expression of DUSP1 was detected using western blot analysis to screen for the one with a higher interference rate...
January 25, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29391488/age-associated-microbiome-shows-the-giant-panda-lives-on-hemicelluloses-not-on-cellulose
#13
Wenping Zhang, Wenbin Liu, Rong Hou, Liang Zhang, Stephan Schmitz-Esser, Huaibo Sun, Junjin Xie, Yunfei Zhang, Chengdong Wang, Lifeng Li, Bisong Yue, He Huang, Hairui Wang, Fujun Shen, Zhihe Zhang
The giant panda feeds almost exclusively on bamboo, a diet highly enriched in lignin and cellulose, but is characterized by a digestive tract similar to carnivores. It is still large unknown if and how the giant panda gut microbiota contributes to lignin and cellulose degradation. Here we show the giant pandas' gut microbiota does not significantly contribute to cellulose and lignin degradation. We found that no operational taxonomic unit had a nearest neighbor identified as a cellulolytic species or strain with a significant higher abundance in juvenile than cubs, a very low abundance of putative lignin and cellulose genes existed in part of analyzing samples but a significant higher abundance of genes involved in starch and hemicellulose degradation in juveniles than cubs...
February 1, 2018: ISME Journal
https://www.readbyqxmd.com/read/29354364/phylogeny-novel-bacterial-lineage-and-enzymatic-potential-of-haloalkaliphilic-bacteria-from-the-saline-coastal-desert-of-little-rann-of-kutch-gujarat-india
#14
Hitarth B Bhatt, Sangeeta D Gohel, Satya P Singh
This report describes cultivation-dependent diversity, phylogeny and enzymatic potential of the haloalkaliphilic bacteria isolated from the unvegetated desert soil of yet unexplored, saline desert of Little Rann of Kutch (LRK), India. The LRK is a unique ecosystem displaying a combination of Dry Rann and Wet Rann. A total of 25 bacteria were isolated and characterized on the basis of colony morphology, biochemical profile, sugar utilization, secretion of the extracellular enzymes and antibiotic sensitivity...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29350293/diversity-and-enzymatic-potentialities-of-bacillus-sp-strains-isolated-from-a-polluted-freshwater-ecosystem-in-cuba
#15
Jeny Adina Larrea-Murrell, Marcia Maria Rojas-Badia, Ivette García-Soto, Beatriz Romeu-Alvarez, Tristano Bacchetti, Annika Gillis, Ana Karina Boltes-Espinola, Mayra Heydrich-Perez, Daysi Lugo-Moya, Jacques Mahillon
Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis...
January 19, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29349096/safety-and-efficacy-of-aav-retrograde-pancreatic-ductal-gene-delivery-in-normal-and-pancreatic-cancer-mice
#16
Kayla A Quirin, Jason J Kwon, Arafat Alioufi, Tricia Factora, Constance J Temm, Max Jacobsen, George E Sandusky, Kim Shontz, Louis G Chicoine, K Reed Clark, Joshua T Mendell, Murray Korc, Janaiah Kota
Recombinant adeno-associated virus (rAAV)-mediated gene delivery shows promise to transduce the pancreas, but safety/efficacy in a neoplastic context is not well established. To identify an ideal AAV serotype, route, and vector dose and assess safety, we have investigated the use of three AAV serotypes (6, 8, and 9) expressing GFP in a self-complementary (sc) AAV vector under an EF1α promoter (scAAV.GFP) following systemic or retrograde pancreatic intraductal delivery. Systemic delivery of scAAV9.GFP transduced the pancreas with high efficiency, but gene expression did not exceed >45% with the highest dose, 5 × 1012 viral genomes (vg)...
March 16, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29329608/polyphenols-isolated-from-acacia-mearnsii-bark-with-anti-inflammatory-and-carbolytic-enzyme-inhibitory-activities
#17
Jia Xiong, Mary H Grace, Debora Esposito, Slavko Komarnytsky, Fei Wang, Mary Ann Lila
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264...
November 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29319514/characterization-of-bacteriocin-producing-lactic-acid-bacteria-isolated-from-native-fruits-of-ecuadorian-amazon
#18
Karina Garzón, Clara Ortega, Gabriela N Tenea
Tropical, wild-type fruits are considered biodiverse "hotspots" of microorganisms with possible functional characteristics to be investigated. In this study, several native lactic acid bacteria (LAB) of Ecuadorian Amazon showing highly inhibitory potential were identified and characterized. Based on carbohydrate fermentation profile and 16S rRNA gene sequencing, seven strains were assigned as Lactobacillus plantarum and one strain as Weissella confusa. Using agar-well diffusion method the active synthetized components released in the neutralized and hydroxide peroxide eliminated cell-free supernatant were inhibited by proteolytic enzymes, while the activity was maintained stable after the treatment with catalase, lysozyme, α-amylase and lipase suggesting their proteinaceous nature...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29250543/efficient-expression-of-maltohexaose-forming-%C3%AE-amylase-from-bacillus-stearothermophilus-in-brevibacillus-choshinensis-sp3-and-its-use-in-maltose-production
#19
Zhu Li, Lingqia Su, Xuguo Duan, Dan Wu, Jing Wu
The maltohexaose-forming, Ca2+-independent α-amylase gene from Bacillus stearothermophilus (AmyMH) was efficiently expressed in Brevibacillus choshinensis SP3. To improve the production of AmyMH in B. choshinensis SP3, the temperature and initial pH of culture medium were optimized. In addition, single-factor and response surface methodologies were pursued to optimize culture medium. Addition of proline to the culture medium significantly improved the production of recombinant α-amylase in B. choshinensis SP3...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29246326/efficacy-and-safety-of-pancreas-targeted-hydrodynamic-gene-delivery-in-rats
#20
Kohei Ogawa, Kenya Kamimura, Yuji Kobayashi, Hiroyuki Abe, Takeshi Yokoo, Norihiro Sakai, Takuro Nagoya, Akira Sakamaki, Satoshi Abe, Kazunao Hayashi, Satoshi Ikarashi, Junji Kohisa, Masanori Tsuchida, Yutaka Aoyagi, Guisheng Zhang, Dexi Liu, Shuji Terai
Development of an effective, safe, and convenient method for gene delivery to the pancreas is a critical step toward gene therapy for pancreatic diseases. Therefore, we tested the possibility of applying the principle of hydrodynamic gene delivery for successful gene transfer to pancreas using rats as a model. The established procedure involves the insertion of a catheter into the superior mesenteric vein with temporary blood flow occlusion at the portal vein and hydrodynamic injection of DNA solution. We demonstrated that our procedure achieved efficient pancreas-specific gene expression that was 2,000-fold higher than that seen in the pancreas after the systemic hydrodynamic gene delivery...
December 15, 2017: Molecular Therapy. Nucleic Acids
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