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gene amylase

A J Myers, A D Gondhalekar, M Fardisi, K D Pluchar, K D Saltzmann, G W Bennett, M E Scharf
German cockroach males possess tergal glands that secrete a combination of oligosaccharides, lipids and proteins. Four major proteins occur in the secretion, with one being the 63 kDa alpha-amylase Blattella germanica Tergal Gland protein-1 (BGTG-1). Denaturing and starch gel electrophoresis coupled with peptide sequencing verified amylase activity for the BGTG-1 protein. BGTG-1 gene expression profiles were determined by using quantitative real-time PCR to compare messenger RNA abundance among isolated tissues of males, females and gravid females...
October 14, 2017: Insect Molecular Biology
Massimiliano Cuccioloni, Matteo Mozzicafreddo, Laura Bonfili, Valentina Cecarini, Mara Giangrossi, Maurizio Falconi, Shin-Ichiroh Saitoh, Anna Maria Eleuteri, Mauro Angeletti
Wheat amylase/trypsin bi-functional inhibitors (ATIs) are protein stimulators of innate immune response, with a recently established role in promoting both gastrointestinal and extra-gastrointestinal inflammatory syndromes. These proteins have been reported to trigger downstream intestinal inflammation upon activation of TLR4, a member of the Toll-like family of proteins that activates signalling pathways and induces the expression of immune and pro-inflammatory genes. In this study, we demonstrated the ability of ATI to directly interact with TLR4 with nanomolar affinity, and we kinetically and structurally characterized the interaction between these macromolecules by means of a concerted approach based on surface plasmon resonance binding analyses and computational studies...
October 13, 2017: Scientific Reports
Alex Z Zaccaron, Charles P Woloshuk, Burton H Bluhm
Stenocarpella maydis is a plant pathogenic fungus that causes Diplodia ear rot, one of the most destructive diseases of maize. To date, little information is available regarding the molecular basis of pathogenesis in this organism, in part due to limited genomic resources. In this study, a 54.8 Mb draft genome assembly of S. maydis was obtained with Illumina and PacBio sequencing technologies, and analyzed. Comparative genomic analyses with the predominant maize ear rot pathogens Aspergillus flavus, Fusarium verticillioides, and Fusarium graminearum revealed an expanded set of carbohydrate-active enzymes for cellulose and hemicellulose degradation in S...
November 2017: Fungal Biology
Zayneb ChaÂBene, Imen Rekik Hakim, Agnieszka Rorat, Amine Elleuch, Hafedh Mejdoub, Franck Vandenbulcke
Date palm seeds were exposed to different copper solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas, higher Cu amount (2 mM) significantly inhibited seeds germination, delayed hypocotyl elongation, increased seedling mortality and reduced germination index by more than 90 %. Metal-related toxicity symptoms appeared after 15 days of 2 mM Cu exposure. Biochemical activities, like amylase activity and redox balance elements were examined in order to study the relationship between external Cu amount and internal plant response...
October 12, 2017: Environmental Toxicology and Chemistry
P Rameshreddy, V V Sathibabu Uddandrao, Parim Brahmanaidu, S Vadivukkarasi, Ramavat Ravindarnaik, Pothani Suresh, K Swapna, A Kalaivani, Parimi Parvathi, P Tamilmani, Ganapathy Saravanan
The present study evaluated the effects of asiatic acid (AA), a pentacyclic triterpenoid from Centella asiatica on lipid metabolism parameters in a rat model of obesity induced using a high fat diet (HFD) for 42 days. AA (20 mg/kg body weight [BW]) was administered orally once daily for 42 days, and an orlistat-treated group of rats (10 mg/kg BW) was included for comparison. Changes in BW, blood glucose levels, insulin resistance and leptin, adiponectin, amylase, and lipase levels in the blood; lipid profiles of plasma; liver antioxidants levels; and acetyl CoA carboxylase(ACC), uncoupling protein-2 (UCP2), and carnitine palmitoyltransferase-1 (CPT1) mRNA expression were observed in the experimental rats...
October 9, 2017: Molecular and Cellular Biochemistry
Wei Zhang, Zihao Zhao, Yankun Yang, Xiuxia Liu, Zhonghu Bai
Corynebacterium glutamicum is an attractive host for the production of heterologous proteins despite its traditional use in fermentative production of amino acids. To enhance the expression levels of target genes, the development of useful promoters is required in the construction of expression systems. Here, we developed a new promoter, the aph promoter from aminoglycoside-3'-phosphotransferase gene, and used it to construct monocistronic and bicistronic expression systems that host different ribosome binding site (RBS) sequences...
October 3, 2017: Plasmid
A R E O Xavier, E R Lima, A M E Oliveira, L Cardoso, J Santos, C H C Cangussu, L N Leite, M C L Quirino, I G C Júnior, D A Oliveira, M A S Xavier
The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyze the genetic diversity among bacteria of the genus Bacillus sp producer of amylase originated from the soil. To this end, soil samples were collected and submitted to the condition of extreme temperature...
September 27, 2017: Genetics and Molecular Research: GMR
Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
In a previous study, we reported that a double gene deletion mutant for CreA and CreB, which constitute the regulatory machinery involved in carbon catabolite repression, exhibited improved production of α-amylase compared with the wild-type strain and single creA or creB deletion mutants in Aspergillus oryzae. Because A. oryzae can also produce biomass-degrading enzymes, such as xylolytic and cellulolytic enzymes, we examined the production levels of those enzymes in deletion mutants in this study. Xylanase and β-glucosidase activities in the wild-type were hardly detected in submerged culture containing xylose as the carbon source, whereas those enzyme activities were significantly increased in the single creA deletion (ΔcreA) and double creA and creB deletion (ΔcreAΔcreB) mutants...
September 29, 2017: Journal of Bioscience and Bioengineering
Dimitry Y Sorokin, Tatiana V Kolganova, Tatiana V Khijniak, Brian E Jones, Ilya V Kublanov
Alkaline saline soils, known also as "soda solonchaks", represent a natural soda habitat which differs from soda lake sediments by higher aeration and lower humidity. The microbiology of soda soils, in contrast to the more intensively studied soda lakes, remains poorly explored. In this work we investigate the diversity of culturable aerobic haloalkalitolerant bacteria with various hydrolytic activities from soda soils at different locations in Central Asia, Africa, and North America. In total, 179 pure cultures were obtained by using media with various polymers at pH 10 and 0...
2017: PeerJ
Jingjing Liu, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
l-Malate, an important chemical building block, has been widely applied in the food, pharmaceutical, and bio-based materials industries. In previous work, we engineered Aspergillus oryzae by rewiring the reductive tricarboxylic acid pathway to produce l-malate from glucose. To decrease the production cost, here, we further engineered A. oryzae to efficiently produce l-malate directly from corn starch with simultaneous liquefaction-saccharification and fermentation. First, a promoter PN5 was constructed by quintuple tandem of the 97-bp fragment containing the cis-element of the glucoamylase gene promoter (PglaA), and with the promoter PN5, the transcriptional level of glaA gene increased by 25-45%...
September 28, 2017: Journal of Biotechnology
Juan José Montor-Antonio, Sarahi Hernández-Heredia, Ángela Ávila-Fernández, Clarita Olvera, Bernardo Sachman-Ruiz, Sandra Del Moral
AmyJ33, an α-amylase isolated from Bacillus amyloliquefaciens JJC33M, has been characterized as a non-metalloenzyme that hydrolyzes raw starch. In this work, the gene that codifies for AmyJ33 was isolated and cloned. The recombinant α-amylase (AmyJ33r) produced had a molecular weight of 72 kDa, 25 kDa higher than the native α-amylase (AmyJ33). Our results suggest that the C-terminal was processed in a different way in the native and the recombinant enzyme causing the difference observed in the molecular weight...
October 2017: 3 Biotech
Xiaojian Wu, Kangfeng Cai, Guoping Zhang, Fanrong Zeng
Grain weight and protein content will be reduced and increased, respectively, when barley is subjected to water stress after anthesis, consequently deteriorating the malt quality. However, such adverse impact of water stress differs greatly among barley genotypes. In this study, two Tibetan wild barley accessions and two cultivated varieties differing in water stress tolerance were used to investigate the genotypic difference in metabolic profiles during grain-filling stage under drought condition. Totally, 71 differently accumulated metabolites were identified, including organic acids, amino acids/amines, and sugars/sugar alcohols...
2017: Frontiers in Plant Science
Hamed Kolangi Miandare, Ali Taheri Mirghaed, Marjan Hosseini, Nastaran Mazloumi, Ashkan Zargar, Sajad Nazari
Pacific white shrimp Litopenaeus vannamei (Boone, 1931) is an important economical shrimp species worldwide, especially in the Middle East region, and farming activities of this species have been largely affected by diseases, mostly viral and bacterial diseases. Scientists have started to use prebiotics for bolstering the immune status of the animal. This study aimed to investigate the influence of Immunogen(®) on growth, digestive enzyme activity and immune related gene expression of Litopenaeus vannamei post-larvae...
September 19, 2017: Fish & Shellfish Immunology
Fen Gao, Rui Hao, Xue-Mei Qin, Zhen-Hong Lei, Yu-Long Wang, Meng-Liang Wang
The antagonistic effect of Bacillus spp. against Fusarium solani was evaluated by living body dual culture and Oxford cup method. The plant growth promoting properties of those strains that had obvious and stable antifungal activity were then tested. The results showed that the living body and bacteria-free fermentation filtrate of strain G10 both had obvious and stable antifungal effect to F. solani. Besides, the strain possessed such growth promoting properties as phosphate solubilization, nitrogen fixation, and production of IAA, amylase and HCN...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Majida Atta Muhammad, Samia Falak, Naeem Rashid, Qurra-Tul-Ann Afza Gardner, Nasir Ahmad, Tadayuki Imanaka, Muhammad Akhtar
Tk1884, an open reading frame encoding α-amylase in Thermococcus kodakarensis, was cloned with the native signal sequence and expressed in Escherichia coli. Heterologous gene expression resulted in secretion of the recombinant protein to the extracellular culture medium. Extracellular α-amylase activity gradually increased after induction. Tk1884 was purified from the extracellular medium, and its molecular mass determined by electrospray ionization mass spectrometry indicated the cleavage of a few amino acids...
July 2017: Biochemistry. Biokhimii︠a︡
Weiwei Xiao, Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang, Yongan Zhang, Xiaoqiu Zhou
This study was conducted to evaluate the effect of enzyme-treated soy protein (ETSP) supplementation in the low-protein diet on growth performance, digestive and absorptive capacities, and related signaling molecules' gene expressions in juvenile Jian carp. The results showed that percent weight gain (PWG), specific growth rate (SGR), and feed intake (FI) were decreased by reducing dietary protein from 34 to 32% (P < 0.05). Supplying low-protein diet with optimal ETSP increased previously mentioned indices of juvenile Jian carp (P < 0...
December 2017: Fish Physiology and Biochemistry
Xiaolei Li, Yujuan Wang, Jong-Tae Park, Liwei Gu, Dan Li
The most extremely thermostable maltogenic amylase (SMMA) from archaeon Staphylothermus marinus has many potential applications in food processing. To ensure safety of microbial origin, a recombinant plasmid containing the enzymic gene and a constitutive promoter AmyR2 was constructed, and then transformed into a GRAS microorganism Bacillus subtilis. The purified SMMA from the liquid cultures of Bacillus has a specific activity of 66.96U/mg, two times more than that from Escherichia coli. SMMA was further employed to catalyze the genistion glycosylation using γ-CD as both glucosyl donors and solubilizer...
September 5, 2017: International Journal of Biological Macromolecules
Ilka Braumann, Christoph Dockter, Sebastian Beier, Axel Himmelbach, Finn Lok, Udda Lundqvist, Birgitte Skadhauge, Nils Stein, Shakhira Zakhrabekova, Ruonan Zhou, Mats Hansson
BACKGROUND: Short-culm mutants have been widely used in breeding programs to increase lodging resistance. In barley (Hordeum vulgare L.), several hundreds of short-culm mutants have been isolated over the years. The objective of the present study was to identify the Brachytic1 (Brh1) semi-dwarfing gene and to test its effect on yield and malting quality. RESULTS: Double-haploid lines generated through a cross between a brh1.a mutant and the European elite malting cultivar Quench, showed good malting quality but a decrease in yield...
2018: Hereditas
J C M Dornelas, J E F Figueiredo, C S de Abreu, U G P Lana, C A Oliveira, I E Marriel
Secondary metabolites produced by Actinobacteria of tropical soils represent a largely understudied source of novel molecules with relevant application in medicine, pharmaceutical and food industries, agriculture, and environmental bioremediation. The present study aimed to characterize sixty-nine Actinobacteria isolated from compost and tropical soils using morphological, biochemical, and molecular methods. All the isolates showed high variation for morphological traits considering the color of pigments of the aerial and vegetative mycelium and spore chain morphology...
August 31, 2017: Genetics and Molecular Research: GMR
Chanjuan Zhao, Junqi Xie, Li Li, Chongjiang Cao
The transcriptomes of paddy rice in response to high temperature and humidity were studied using a high-throughput RNA sequencing approach. Effects of high temperature and humidity on the sucrose and starch contents and α/β-amylase activity were also investigated. Results showed that 6876 differentially expressed genes (DEGs) were identified in paddy rice under high temperature and humidity storage. Importantly, 12 DEGs that were downregulated fell into the "starch and sucrose pathway". The quantitative real-time polymerase chain reaction assays indicated that expression of these 12 DEGs was significantly decreased, which was in parallel with the reduced level of enzyme activities and the contents of sucrose and starch in paddy rice stored at high temperature and humidity conditions compared to the control group...
September 7, 2017: Journal of Agricultural and Food Chemistry
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