keyword
MENU ▼
Read by QxMD icon Read
search

Chondrocyte flow cytometry

keyword
https://www.readbyqxmd.com/read/29331857/baicalin-alleviates-il-1%C3%AE-induced-inflammatory-injury-via-down-regulating-mir-126-in-chondrocytes
#1
Xirui Yang, Qi Zhang, Zhaomeng Gao, Chunyan Yu, Lei Zhang
Baicalin is a flavonoid extracted from Scutellaria baicalensis Georgi, with anti-inflammatory and anti-apoptotic activities. The objective of this study was to explore the effect and mechanism of baicalin on chondrocyte inflammatory response in OA. Different concentrations of IL-1β (0, 0.1, 2, 5 and 10 ng/mL) were used to simulate inflammatory injury in CHON-001 cells. The expression of miR-126 was altered by transfection with miR-126 mimic. Thereafter, cells were treated with baicalin, and cell viability, apoptosis, the expressions of apoptosis-related protein and pro-inflammatory factors were respectively detected using CCK-8 assay, flow cytometry, qRT-PCR and western blot analysis...
January 11, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29328479/the-human-umbilical-cord-stem-cells-improve-the-viability-of-oa-degenerated-chondrocytes
#2
Hao Wang, Xu Yan, Yuxin Jiang, Zheng Wang, Yufei Li, Qingdong Shao
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim of the present study was to explore the interaction between human umbilical cord stem cells and degenerated chondrocytes, and the therapeutic potential of human umbilical cord stem cells on degenerated chondrocytes. Human umbilical cord‑derived mesenchymal stem cells (hUC‑MSCs) were harvested from human umbilical cords, and flow cytometry was used to analyze the surface antigen markers, in addition, chondrogenic, osteogenic and adipogenic differentiation on the cells was investigated...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29323717/chondrogenic-differentiation-of-human-scalp-adipose-derived-stem-cells-in-polycaprolactone-scafold-and-using-freeze-thaw-freeze-method
#3
Rana Moradian Tehrani, Hamed Mirzaie, Javad Verdi, Amirhossein Sahebkar, Mahdi Noureddini, Rasoul Salehi, Behrang Alani, Mojtaba Kianmehr
Human adipose tissue has been identified as a viable alternative source for mesenchymal stem cells. SADSCs were isolated from human scalp biopsy and then were characterized by Flow cytometry. SADSCS expressed CD90, CD44, and CD105 but did not express CD45 surface marker. Growth factors were used for chondrogenesis induction. Histology and immunohistology methods and gene expression by real-time PCR 14 days after induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29306212/microrna-19a-promotes-cell-viability-and-migration-of-chondrocytes-via-up-regulating-sox9-through-nf-%C3%AE%C2%BAb-pathway
#4
Chuandong Yu, Yongkun Wang
BACKGROUND: Osteoarthritis (OA), as a degenerative disease, is a major problem in ageing populations. To better understand the underlying mechanisms in the pathogenesis of OA, this study was undertaken to investigate the role of microRNA (miR)-19a in chondrocytes. METHODS: Expression of the members of miR-17-92 cluster in synovium from OA patients and non-OA patients were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). miR-19a was abnormal expressed in human chondrocyte line (CHON-001 and T-C/28 cells) and primary human chondrocytes by transient transfection...
January 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29217163/cx3cr1-regulates-osteoarthrosis-chondrocyte-proliferation-and-apoptosis-via-wnt-%C3%AE-catenin-signaling
#5
You Sun, Feifei Wang, Xuehui Sun, Xiaoxue Wang, Lei Zhang, Yang Li
OBJECTIVE: The study was aimed to investigate the impact of CX3CR1 expression on the proliferation and apoptosis of osteoarthrosis (OA) chondrocytes through Wnt/ β-catenin pathway. METHODS: The expression levels of CX3CR1 and Wnt/β-catenin pathway-related genes mRNA and protein in OA chondrocytes were examined by qRT-PCR and western blot. MTT and flow cytometry (FCM) assays were employed to assess cell proliferation, cell cycle and apoptosis. XAV-939, a Wnt/β-catenin pathway inhibitor, was used to inhibit the pathway...
December 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29204860/dec1-is-required-for-anti-apoptotic-activity-of-gastric-cancer-cells-under-hypoxia-by-promoting-survivin-expression
#6
Yanfei Jia, Rui Hu, Ping Li, Yan Zheng, Yunshan Wang, Xiaoli Ma
BACKGROUND: Human differentiated embryonic chondrocyte-expressed gene 1 (DEC1), which has been reported to be overexpressed in several types of cancer, is associated with tumorigenesis through participation in several biological processes. However, the complex mechanisms underlying DEC1 during carcinogenesis are controversial, and its roles in the development and malignancy of gastric cancer (GC) remain unclear. METHODS: We measured DEC1 expression in human GC cell lines...
December 4, 2017: Gastric Cancer
https://www.readbyqxmd.com/read/29179481/notochordal-cell-derived-extracellular-vesicles-exert-regenerative-effects-on-canine-and-human-nucleus-pulposus-cells
#7
Frances Bach, Sten Libregts, Laura Creemers, Björn Meij, Keita Ito, Marca Wauben, Marianna Tryfonidou
During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal cells (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by NCs may be responsible for these effects, we characterized NC-derived extracellular vesicles (EVs) and determined their effect on CLCs. EVs were purified from porcine NC-conditioned medium (NCCM) through size exclusion chromatography, ultracentrifugation or density gradient centrifugation. Additionally, the EVs were quantitatively analyzed by high-resolution flow cytometry...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29143973/effects-of-microrna-24-targeting-c-myc-on-apoptosis-proliferation-and-cytokine-expressions-in-chondrocytes-of-rats-with-osteoarthritis-via-mapk-signaling-pathway
#8
Yuan-Hao Wu, Wei Liu, Lei Zhang, Xiao-Ya Liu, Yi Wang, Bin Xue, Bin Liu, Ran Duan, Bo Zhang, Yang Ji
OBJECTIVE: To investigate whether microRNA-24 (miR-24) targeting C-myc affects chondrocytes of rats with osteoarthritis (OA) via the MAPK signaling pathway. METHODS: Thirty rats were assigned as a sham group and an OA group (established as OA rat models by cutting the anterior cruciate ligaments and removing 1/3 medial meniscus). TUNEL staining and immunohistochemistry were conducted for cell apoptosis index (AI) and positive expression rate of C-myc protein. Enzyme-linked immuno sorbent assay (ELISA) was carried out for serum level of IL-1β and TNF-α...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29118937/isolation-differentiation-and-characterization-of-mesenchymal-stem-cells-from-human-bone-marrow
#9
Kaveh Baghaei, Seyed Mohmoud Hashemi, Samaneh Tokhanbigli, Ali Asadi Rad, Hamid Assadzadeh-Aghdaei, Abdolhamid Sharifian, Mohammad Reza Zali
Aim: We describe the minimum requirements and a simplified method for isolation and characterization of mesenchymal stem cells (MSCs) from human bone marrow. Background: MSCs are well known adult stem cells present in many tissues such as adipocytes, chondrocytes, osteoblasts, and neurons. Many isolations and characterization methods have emerged to apply MSCs in the clinical applications, which many of them are expensive and time-consuming. Methods: MSC isolation was carried out from human bone marrow, and cultured in defined medium...
2017: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/29104661/the-molecular-mechanism-of-treating-osteoarthritis-with-dipsacus-saponins-by-inhibiting-chondrocyte-apoptosis
#10
Xian-Rang Li, Jian Li, Qiang Ren, Shui Sun
The present study aimed to determine the molecular mechanism of treating osteoarthritis with dipsacus saponins by inhibiting the apoptosis of chondrocytes. A total of 30 New Zealand rabbits were randomly divided into 2 groups: A control group and a model group. The osteoarthritis model was established using the HULTH method. The success of the model establishment was determined by pathological morphology and measurement of inflammatory cytokine levels. Chondrocytes were isolated and divided into 4 groups treated with varying concentrations of dipsacus saponins: 0, 25, 50 and l00 µg/l dipsacus saponins...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29081152/-mir-30a-5p-promotes-the-apoptosis-of-chondrocytes-in-patients-with-osteoarthritis-by-targeting-protein-kinase-b
#11
P F Shen, Y X Qu, B Wang, J D Xu, K Wei, Z K Xie, Y Ma
Objective: To investigate the expression of miR-30a-5p in cartilage of osteoarthritis patients, and to explore its mechanism of chondrocyte apoptosis. Methods: From May 2015 to December 2016, tissue specimen of 289 patients with osteoarthritis was collected in Department of Orthopedics, Changzhou traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine.The expression of miR-30a-5p and protein kinase B(Akt) mRNA in cartilage of different patients was detected by qPCR.The apoptosis of chondrocytes was detected by Tunel method...
October 24, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29061447/pdk1-promotes-apoptosis-of-chondrocytes-via-modulating-mapk-pathway-in-osteoarthritis
#12
Qingtian Ge, Hailin Wang, Xinbao Xu, Libin Xu, Leilei Zhai, Ran Tao
3-Phosphoinositide dependent protein kinase-1 (PDK1), a serine threonine kinase, belongs to the AGC kinase family and is associated with apoptosis. The aim of this study was to investigate the expression of PDK1 (3-Phosphoinositide dependent protein kinase-1) in articular cartilage with osteoarthritis (OA) and to analyze the relationship between PDK1 and chondrocyte apoptosis. Immunohistochemistry and RT-PCR analysis showed that the expression of PDK1 in articular cartilage of OA patients and healthy controls...
December 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29050153/-study-on-the-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells-into-chondrocytes-induced-by-tgf-beta-3
#13
D B Liu, X S Han, W L Huang, J Deng, R F She
Objective: To explore the methods for the differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) into chondrocytes induced by transforming growth factor (TGF)-beta 3 in vitro, and search for a reliable seed cell for the regeneration and repair of articular cartilage in osteoarthritis. Methods: SD rat femoral and tibial BMMSCs were cultured by the whole bone marrow adherent method, and then the purity was identified by flow cytometry. P3 generation cells were induced and differentiated into chondrocytes by the induction of differentiation medium containing TGF-beta 3, and cell chondrogenic differentiation ability at different induction time points was detected...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29039445/isoliquiritigenin-suppresses-il-1%C3%AE-induced-apoptosis-and-inflammation-in-chondrocyte-like-atdc5-cells-by-inhibiting-nf-%C3%AE%C2%BAb-and-exerts-chondroprotective-effects-on-a-mouse-model-of-anterior-cruciate-ligament-transection
#14
Baochao Ji, Wentao Guo, Hairong Ma, Boyong Xu, Wenbo Mu, Zhendong Zhang, Abdusami Amat, Li Cao
Isoliquiritigenin (ISL), a natural flavonoid extracted from licorice, has been demonstrated to exert attenuation of the nuclear factor-κB (NF-κB) signaling pathway and anti-inflammatory activity in a wide variety of cells. In the present study, the authors first evaluated the effects of ISL on cartilage degeneration in interleukin-1β (IL-1β)-stimulated chondrocyte-like ATDC5 cells and in a mouse model of osteoarthritis (OA). The data of a cell counting kit-8 and flow cytometry assay indicated that ISL suppressed the inhibitory effect of IL-1β on cell viability...
October 9, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28962125/optimization-of-pure-platelet-rich-plasma-preparation-a-comparative-study-of-pure-platelet-rich-plasma-obtained-using-different-centrifugal-conditions-in-a-single-donor-model
#15
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhenzhong Zhu, Dongxu Jin, Peichun Hsu, Xuetao Xie, Changqing Zhang
While it has been proved that centrifugal conditions for pure platelet-rich plasma (P-PRP) preparation influence the cellular composition of P-PRP obtained, the optimal centrifugal conditions to prepare P-PRP have not yet been identified. In the present study, platelet-containing plasma (PCP) was prepared with the first-spin of different double-spin methods and P-PRP was prepared with different double-spin methods. Whole-blood analysis was performed to evaluate the cellular composition of PCP and P-PRP. The basal and ADP-induced CD62P expression rates of platelets were assessed by flow cytometry to evaluate the function of platelets in PCP and P-PRP...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28957801/globular-adiponectin-attenuated-h2o2-induced-apoptosis-in-rat-chondrocytes-by-inducing-autophagy-through-the-ampk-mtor-pathway
#16
Junzheng Hu, Weiding Cui, Wenxiao Ding, Yanqing Gu, Zhen Wang, Weimin Fan
BACKGROUND/AIMS: Chondrocyte apoptosis is closely related to the development and progression of osteoarthritis. Global adiponectin (gAPN), secreted from adipose tissue, possesses potent anti-inflammatory and antiapoptotic properties in various cell types. This study aimed to investigate the role of autophagy induced by gAPN in the suppression of H2O2-induced apoptosis and the potential mechanism of gAPN-induced autophagy in chondrocytes. METHODS: H2O2 was used to induce apoptotic injury in rat chondrocytes...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28931727/co-culture-of-allogenic-dbm-and-bmscs-in-the-knee-joint-cavity-of-rabbits-for-cartilage-tissue-engineering
#17
Bin Xu, Rui Wang, Hao Wang, Hong-Gang Xu
This study aims to assess co-culture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in-vitro group, an in-vivo group and a blank control group. At the 4(th), 8(th) and 12(th) week, samples from all groups were collected for hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value)...
September 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28817810/tgf-%C3%AE-1-is-involved-in-vitamin-d-induced-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-by-regulating-the-erk-jnk-pathway
#18
Xiaorui Jiang, Botao Huang, Huiying Yang, Guishi Li, Chunlei Zhang, Guangshi Yang, Feng Lin, Guodong Lin
BACKGROUND/AIMS: Osteoarthritis (OA) is characterized by degradation of cartilage, sole cell type of which is chondrocytes. Bone marrow-derived mesenchymal stem cells (BMSCs) possess multipotency and can be directionally differentiated into chondrocytes under stimulation. This study was aimed to explore the possible roles of vitamin D and transforming growth factor-β1 (TGF-β1) in the chondrogenic differentiation of BMSCs. METHODS: BMSCs were isolated from femurs and tibias of rats and characterized by flow cytometry...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28810862/the-cytotoxic-mechanism-of-epigallocatechin-gallate-on-proliferative-hacat-keratinocytes
#19
Yu-Wen Chu, Shu-Ting Liu, Ya-Lan Yang, Shih-Ming Huang, Wei-Ming Wang
BACKGROUND: Epigallocatechin gallate (EGCG) is the major ingredient of sinecatechins ointment, approved for the treatment of external genital and perianal warts. However, the molecular mechanism for EGCG's effect on warts resulting from the human papillomavirus (HPV) infection of keratinocytes is not well understood. HPV may survive in proliferative keratinocytes and may be involved in cell cycle regulation and progression. The objective of this study was to investigate the mechanism underlying EGCG's treatment on external genital warts of HPV infection through the cultured keratinocyte cells from the HaCaT cell line...
August 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28801211/parkin-clearance-of-dysfunctional-mitochondria-regulates-ros-levels-and-increases-survival-of-human-chondrocytes
#20
M Y Ansari, N M Khan, I Ahmad, T M Haqqi
OBJECTIVE: Mitochondrial dysfunction, oxidative stress and chondrocyte death are important contributors to the development and pathogenesis of osteoarthritis (OA). In this study, we determined the expression and role of Parkin in the clearance of damaged/dysfunctional mitochondria, regulation of reactive oxygen species (ROS) levels and chondrocyte survival under pathological conditions. METHODS: Human chondrocytes were from the unaffected area of knee OA cartilage (n = 12) and were stimulated with IL-1β to mimic pathological conditions...
August 8, 2017: Osteoarthritis and Cartilage
keyword
keyword
43709
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"