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Chondrocyte flow cytometry

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https://www.readbyqxmd.com/read/29143973/effects-of-microrna-24-targeting-c-myc-on-apoptosis-proliferation-and-cytokine-expressions-in-chondrocytes-of-rats-with-osteoarthritis-via-mapk-signaling-pathway
#1
Yuan-Hao Wu, Wei Liu, Lei Zhang, Xiao-Ya Liu, Yi Wang, Bin Xue, Bin Liu, Ran Duan, Bo Zhang, Yang Ji
OBJECTIVE: To investigate whether microRNA-24 (miR-24) targeting C-myc affects chondrocytes of rats with osteoarthritis (OA) via the MAPK signaling pathway. METHODS: Thirty rats were assigned as a sham group and an OA group (established as OA rat models by cutting the anterior cruciate ligaments and removing 1/3 medial meniscus). TUNEL staining and immunohistochemistry were conducted for cell apoptosis index (AI) and positive expression rate of C-myc protein. Enzyme-linked immuno sorbent assay (ELISA) was carried out for serum level of IL-1β and TNF-α...
November 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29118937/isolation-differentiation-and-characterization-of-mesenchymal-stem-cells-from-human-bone-marrow
#2
Kaveh Baghaei, Seyed Mohmoud Hashemi, Samaneh Tokhanbigli, Ali Asadi Rad, Hamid Assadzadeh-Aghdaei, Abdolhamid Sharifian, Mohammad Reza Zali
Aim: We describe the minimum requirements and a simplified method for isolation and characterization of mesenchymal stem cells (MSCs) from human bone marrow. Background: MSCs are well known adult stem cells present in many tissues such as adipocytes, chondrocytes, osteoblasts, and neurons. Many isolations and characterization methods have emerged to apply MSCs in the clinical applications, which many of them are expensive and time-consuming. Methods: MSC isolation was carried out from human bone marrow, and cultured in defined medium...
2017: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/29104661/the-molecular-mechanism-of-treating-osteoarthritis-with-dipsacus-saponins-by-inhibiting-chondrocyte-apoptosis
#3
Xian-Rang Li, Jian Li, Qiang Ren, Shui Sun
The present study aimed to determine the molecular mechanism of treating osteoarthritis with dipsacus saponins by inhibiting the apoptosis of chondrocytes. A total of 30 New Zealand rabbits were randomly divided into 2 groups: A control group and a model group. The osteoarthritis model was established using the HULTH method. The success of the model establishment was determined by pathological morphology and measurement of inflammatory cytokine levels. Chondrocytes were isolated and divided into 4 groups treated with varying concentrations of dipsacus saponins: 0, 25, 50 and l00 µg/l dipsacus saponins...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29081152/-mir-30a-5p-promotes-the-apoptosis-of-chondrocytes-in-patients-with-osteoarthritis-by-targeting-protein-kinase-b
#4
P F Shen, Y X Qu, B Wang, J D Xu, K Wei, Z K Xie, Y Ma
Objective: To investigate the expression of miR-30a-5p in cartilage of osteoarthritis patients, and to explore its mechanism of chondrocyte apoptosis. Methods: From May 2015 to December 2016, tissue specimen of 289 patients with osteoarthritis was collected in Department of Orthopedics, Changzhou traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine.The expression of miR-30a-5p and protein kinase B(Akt) mRNA in cartilage of different patients was detected by qPCR.The apoptosis of chondrocytes was detected by Tunel method...
October 24, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29061447/pdk1-promotes-apoptosis-of-chondrocytes-via-modulating-mapk-pathway-in-osteoarthritis
#5
Qingtian Ge, Hailin Wang, Xinbao Xu, Libin Xu, Leilei Zhai, Ran Tao
3-Phosphoinositide dependent protein kinase-1 (PDK1), a serine threonine kinase, belongs to the AGC kinase family and is associated with apoptosis. The aim of this study was to investigate the expression of PDK1 (3-Phosphoinositide dependent protein kinase-1) in articular cartilage with osteoarthritis (OA) and to analyze the relationship between PDK1 and chondrocyte apoptosis. Immunohistochemistry and RT-PCR analysis showed that the expression of PDK1 in articular cartilage of OA patients and healthy controls...
October 18, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/29050153/-study-on-the-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells-into-chondrocytes-induced-by-tgf-beta-3
#6
D B Liu, X S Han, W L Huang, J Deng, R F She
Objective: To explore the methods for the differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) into chondrocytes induced by transforming growth factor (TGF)-beta 3 in vitro, and search for a reliable seed cell for the regeneration and repair of articular cartilage in osteoarthritis. Methods: SD rat femoral and tibial BMMSCs were cultured by the whole bone marrow adherent method, and then the purity was identified by flow cytometry. P3 generation cells were induced and differentiated into chondrocytes by the induction of differentiation medium containing TGF-beta 3, and cell chondrogenic differentiation ability at different induction time points was detected...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29039445/isoliquiritigenin-suppresses-il-1%C3%AE-induced-apoptosis-and-inflammation-in-chondrocyte-like-atdc5-cells-by-inhibiting-nf-%C3%AE%C2%BAb-and-exerts-chondroprotective-effects-on-a-mouse-model-of-anterior-cruciate-ligament-transection
#7
Baochao Ji, Wentao Guo, Hairong Ma, Boyong Xu, Wenbo Mu, Zhendong Zhang, Abdusami Amat, Li Cao
Isoliquiritigenin (ISL), a natural flavonoid extracted from licorice, has been demonstrated to exert attenuation of the nuclear factor-κB (NF-κB) signaling pathway and anti-inflammatory activity in a wide variety of cells. In the present study, the authors first evaluated the effects of ISL on cartilage degeneration in interleukin-1β (IL-1β)-stimulated chondrocyte-like ATDC5 cells and in a mouse model of osteoarthritis (OA). The data of a cell counting kit-8 and flow cytometry assay indicated that ISL suppressed the inhibitory effect of IL-1β on cell viability...
October 9, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28962125/optimization-of-pure-platelet-rich-plasma-preparation-a-comparative-study-of-pure-platelet-rich-plasma-obtained-using-different-centrifugal-conditions-in-a-single-donor-model
#8
Wenjing Yin, Haitao Xu, Jiagen Sheng, Zhenzhong Zhu, Dongxu Jin, Peichun Hsu, Xuetao Xie, Changqing Zhang
While it has been proved that centrifugal conditions for pure platelet-rich plasma (P-PRP) preparation influence the cellular composition of P-PRP obtained, the optimal centrifugal conditions to prepare P-PRP have not yet been identified. In the present study, platelet-containing plasma (PCP) was prepared with the first-spin of different double-spin methods and P-PRP was prepared with different double-spin methods. Whole-blood analysis was performed to evaluate the cellular composition of PCP and P-PRP. The basal and ADP-induced CD62P expression rates of platelets were assessed by flow cytometry to evaluate the function of platelets in PCP and P-PRP...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28957801/globular-adiponectin-attenuated-h2o2-induced-apoptosis-in-rat-chondrocytes-by-inducing-autophagy-through-the-ampk-mtor-pathway
#9
Junzheng Hu, Weiding Cui, Wenxiao Ding, Yanqing Gu, Zhen Wang, Weimin Fan
BACKGROUND/AIMS: Chondrocyte apoptosis is closely related to the development and progression of osteoarthritis. Global adiponectin (gAPN), secreted from adipose tissue, possesses potent anti-inflammatory and antiapoptotic properties in various cell types. This study aimed to investigate the role of autophagy induced by gAPN in the suppression of H2O2-induced apoptosis and the potential mechanism of gAPN-induced autophagy in chondrocytes. METHODS: H2O2 was used to induce apoptotic injury in rat chondrocytes...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28931727/co-culture-of-allogenic-dbm-and-bmscs-in-the-knee-joint-cavity-of-rabbits-for-cartilage-tissue-engineering
#10
Bin Xu, Rui Wang, Hao Wang, Hong-Gang Xu
This study aims to assess co-culture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in-vitro group, an in-vivo group and a blank control group. At the 4(th), 8(th) and 12(th) week, samples from all groups were collected for hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value)...
September 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28817810/tgf-%C3%AE-1-is-involved-in-vitamin-d-induced-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-by-regulating-the-erk-jnk-pathway
#11
Xiaorui Jiang, Botao Huang, Huiying Yang, Guishi Li, Chunlei Zhang, Guangshi Yang, Feng Lin, Guodong Lin
BACKGROUND/AIMS: Osteoarthritis (OA) is characterized by degradation of cartilage, sole cell type of which is chondrocytes. Bone marrow-derived mesenchymal stem cells (BMSCs) possess multipotency and can be directionally differentiated into chondrocytes under stimulation. This study was aimed to explore the possible roles of vitamin D and transforming growth factor-β1 (TGF-β1) in the chondrogenic differentiation of BMSCs. METHODS: BMSCs were isolated from femurs and tibias of rats and characterized by flow cytometry...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28810862/the-cytotoxic-mechanism-of-epigallocatechin-gallate-on-proliferative-hacat-keratinocytes
#12
Yu-Wen Chu, Shu-Ting Liu, Ya-Lan Yang, Shih-Ming Huang, Wei-Ming Wang
BACKGROUND: Epigallocatechin gallate (EGCG) is the major ingredient of sinecatechins ointment, approved for the treatment of external genital and perianal warts. However, the molecular mechanism for EGCG's effect on warts resulting from the human papillomavirus (HPV) infection of keratinocytes is not well understood. HPV may survive in proliferative keratinocytes and may be involved in cell cycle regulation and progression. The objective of this study was to investigate the mechanism underlying EGCG's treatment on external genital warts of HPV infection through the cultured keratinocyte cells from the HaCaT cell line...
August 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28801211/parkin-clearance-of-dysfunctional-mitochondria-regulates-ros-levels-and-increases-survival-of-human-chondrocytes
#13
M Y Ansari, N M Khan, I Ahmad, T M Haqqi
OBJECTIVE: Mitochondrial dysfunction, oxidative stress and chondrocyte death are important contributors to the development and pathogenesis of osteoarthritis (OA). In this study, we determined the expression and role of Parkin in the clearance of damaged/dysfunctional mitochondria, regulation of reactive oxygen species (ROS) levels and chondrocyte survival under pathological conditions. METHODS: Human chondrocytes were from the unaffected area of knee OA cartilage (n = 12) and were stimulated with IL-1β to mimic pathological conditions...
August 8, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28761446/characteristics-of-human-endometrium-derived-mesenchymal-stem-cells-and-their-tropism-to-endometriosis
#14
Yan Cheng, Liru Li, Dejun Wang, Qiuyan Guo, Yanan He, Tian Liang, Liyuan Sun, Xiaojun Wang, Yulei Cheng, Guangmei Zhang
Human endometrial tissue has become an attractive source of mesenchymal stem cells (MSCs) for cell-based therapies because these MSCs can be easily harvested and have tumour tropism as well as reduced immunogenic and inflammatory properties. Our study aimed to obtain and characterise human endometrial mesenchymal stem cells (EMSCs) and assess their endometriosis tropism. EMSCs were successfully isolated from the endometrium of women undergoing laparoscopy for idiopathic infertility. The EMSCs presented a fibroblast-like morphology during culture...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28744712/vacuolar-protein-sorting-4b-vps4b-regulates-apoptosis-of-chondrocytes-via-p38-mitogen-activated-protein-kinases-mapk-in-osteoarthritis
#15
Libin Xu, Leilei Zhai, Qingtian Ge, Zhongbing Liu, Ran Tao
To aim of this study is to investigate the expression of VPS4B (vacuolar protein sorting 4B) in articular cartilage with osteoarthritis (OA) and to analyze the relationship between VPS4B and chondrocyte apoptosis. We established an OA rat model by the MLI (meniscal/ligamentous injury) modeling method, and we observed the expression of VPS4B in articular cartilage through immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Human SW1353 chondrosarcoma cells were treated with IL-1β to mimic the OA-like chondrocyte injury in vitro, and Western blot was employed to examine the IL-1β-induced expression of VPS4B, phosphorylated p38, and apoptotic markers, namely active caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)...
December 2017: Inflammation
https://www.readbyqxmd.com/read/28685122/nano-engineered-skin-mesenchymal-stem-cells-potential-vehicles-for-tumour-targeted-quantum-dot-delivery
#16
Liga Saulite, Dominyka Dapkute, Karlis Pleiko, Ineta Popena, Simona Steponkiene, Ricardas Rotomskis, Una Riekstina
Nanotechnology-based drug design offers new possibilities for the use of nanoparticles in imaging and targeted therapy of tumours. Due to their tumour-homing ability, nano-engineered mesenchymal stem cells (MSCs) could be utilized as vectors to deliver diagnostic and therapeutic nanoparticles into a tumour. In the present study, uptake and functional effects of carboxyl-coated quantum dots QD655 were studied in human skin MSCs. The effect of QD on MSCs was examined using a cell viability assay, Ki67 expression analysis, and tri-lineage differentiation assay...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28670366/inhibitory-effect-of-dihydroartemisinin-on-chondrogenic-and-hypertrophic-differentiation-of-mesenchymal-stem-cells
#17
Zhen Cao, Chuan Liu, Yun Bai, Ce Dou, Jian-Mei Li, Duo-Wei Shi, Shi-Wu Dong, Qiang Xiang
Chondrocytes located in hyaline cartilage may maintain phenotype while the chondrocytes situated in calcified cartilage differentiate into hypertrophy. Chondrogenic and hypertrophic differentiation of mesenchymal stem cells (MSCs) are two subsequent processes during endochondral ossification. However, it is necessary for chondrocytes to hold homeostasis and to inhibit hypertrophic differentiation in stem cell-based regenerated cartilage. Dihydroartemisinin (DHA) is derived from artemisia apiacea which has many biological functions such as anti-malarial and anti-tumor...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28664842/difference-between-biomarkers-of-tibial-bone-marrow-and-adipose-tissue
#18
Ersin Kuyucu, Mehmet Erdil, Adnan Kara, Murat Bülbül
BACKGROUND: Stem cells, with their regeneration capacity, long-term viability, and differentiation characteristics, have indispensable biological properties. As described by Hauner and Grigoradis et al., mesenchymal stem cell originating from adipose or bone marrow can be differentiated into many tissues such as adipocyte, chondrocyte, myeloblast, and osteoblast. The aim of our study is to compare the use of adipose and tibial bone marrow derived stem cells for therapeutic purposes in orthopedic surgery, which has not been clearly evaluated in the literature to our knowledge and to also evaluate their use...
2017: SICOT-J
https://www.readbyqxmd.com/read/28656222/activation-of-the-toll-like-receptor-8-pathway-increases-the-immunogenicity-of-mesenchymal-stem-cells-from-umbilical-cord
#19
Yu Yang, Yanwen Wang, Li Li, Fei Chen, Peng Zhang
Mesenchymal stem cells (MSCs) are now widely used in clinical cell‑based therapy due to their characteristics of low immunogenicity, multiple differentiation potency and the capability to modulate immune responses. However, accumulated research has indicated the absence of engrafted MSCs because of the increased immunogenicity of MSCs. Toll‑like receptors (TLRs) are essential for the innate immune response and regulating the biological function of MSCs. The present study used human umbilical cord‑derived MSCs (UCMSCs) and activated the TLR8 pathway of UCMSCs to study the role of TLR8 in mediating the immune status of UCMSCs...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28626114/thyroid-stimulating-hormone-stimulation-downregulates-autophagy-and-promotes-apoptosis-in-chondrocytes
#20
Wei Xin, Yue Yu, Yuan Ma, Yuan Gao, Ying Xu, Liyong Chen, Qiang Wan
Subclinical hypothyroidism (SCH) patients have normal thyroid hormone levels but increased thyroid stimulating hormone (TSH) level in serum. It has been reported that high TSH is related to abnormal skeletal development in mice with hypothyroidism. However, the cellular mechanism is not fully understood. In the present study, we aim to investigate the direct effects of TSH stimulation on chondrocytes, and the putative role of autophagy in this process. By using EdU incorporation assay and flow cytometry for mitochondrial membrane potential assay, we demonstrated deceased proliferation and promoted apoptosis in TSH stimulated primary mouse chondrocytes...
June 13, 2017: Endocrine Journal
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