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https://www.readbyqxmd.com/read/28342979/guggulsterone-sensitized-drug-resistant-human-hepatocarcinoma-cells-to-doxorubicin-through-a-cox-2-p-gp-dependent-pathway
#1
Hong-Bin Xu, Jun Fu, Fang Huang, Jing Yu
Previous researches indicated that cyclooxygenase-2 (Cox-2) might be involved in P-glycoprotein (P-gp)-mediated multidrug resistance in hepatocellular carcinoma cells. Doxorubicin-resistant hepatocellular carcinoma PLC/PRF/5 cells (PLC/PRF/5R) and HepG2 (HepG2R) cells were developed in the present study. The modulatory effect of guggulsterone on Cox-2 and P-gp in PLC/PRF/5R and HepG2R cells was investigated. Cells proliferation, Cox-2 and P-gp expression, and prostaglandin E2 release were examined using MTT, flow cytometry, western blot and ELISA assays...
March 22, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28340519/barrington-s-nucleus-neuroanatomic-landscape-of-the-mouse-pontine-micturition-center
#2
Anne M J Verstegen, Veronique Vanderhorst, Paul A Gray, Mark L Zeidel, Joel C Geerling
Barrington's nucleus (Bar) is thought to contain neurons that trigger voiding and thereby function as the "pontine micturition center." Lacking detailed information on this region in mice, we examined gene and protein markers to characterize Bar and the neurons surrounding it. Like rats and cats, mice have an ovoid core of medium-sized Bar neurons located medial to the locus coeruleus (LC). Bar neurons express a GFP reporter for Vglut2, develop from a Math1/Atoh1 lineage, and exhibit immunoreactivity for NeuN...
March 24, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/28338977/effects-of-augmenting-n-methyl-d-aspartate-receptor-signaling-on-working-memory-and-experience-dependent-plasticity-in-schizophrenia-an-exploratory-study-using-acute-d-cycloserine
#3
Jennifer K Forsyth, Peter Bachman, Daniel H Mathalon, Brian J Roach, Elissa Ye, Robert F Asarnow
Cognitive deficits in schizophrenia have been hypothesized to reflect N-methyl-D-aspartate receptor (NMDAR) dysfunction. However, the mechanisms through which the NMDAR contributes to individual cognitive functions differ. To explore how NMDAR signaling relates to specific cognitive deficits in schizophrenia, we tested the effects of enhancing NMDAR signaling on working memory and experience-dependent plasticity using d-cycloserine (DCS). Plasticity was assessed using an EEG paradigm that utilizes high-frequency visual stimulation (HFvS) to induce neural potentiation, and 2 learning tasks, the information integration (IIT) and weather prediction (WPT) tasks...
March 17, 2017: Schizophrenia Bulletin
https://www.readbyqxmd.com/read/28338324/studies-to-examine-potential-tolerability-differences-between-the-5-ht2c-receptor-selective-agonists-lorcaserin-and-cp-809101
#4
Guy A Higgins, Leonardo B Silenieks, Amy Patrick, Ines A M De Lannoy, Paul J Fletcher, Linda A Parker, Neil J MacLusky, Laura C Sullivan, Teresa A Chavera, Kelly A Berg
Lorcaserin (LOR) is a selective 5-HT2C receptor agonist that has been FDA approved as a treatment for obesity. The most frequently reported side-effects of LOR include nausea and headache, which can be dose limiting. We have previously reported that in the rat, while LOR produced unconditioned signs characteristic of nausea/malaise, the highly selective 5-HT2C agonist CP-809101 (CP) produced fewer equivalent signs. Because this may indicate a subclass of 5-HT2C agonists having better tolerability, the present studies were designed to further investigate this apparent difference...
March 24, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28338292/rational-drug-design-of-indazole-based-diarylurea-derivatives-as-anticancer-agents
#5
Yan-Yan Chu, He-Juan Cheng, Zhenhua Tian, Jian-Chun Zhao, Gang Li, Yang-Yang Chu, Chang-Jun Sun, Wen-Bao Li
A series of novel indazole-based diarylurea derivatives targeting c-kit were designed by structure-based drug design. The derivatives were prepared and their antiproliferative activities were evaluated against human colon cancer HCT-116 cell line and hepatocellular carcinoma PLC/PRF/5 cell line. The antiproliferative activities demonstrated that six out of nine compounds exhibited comparable activities with Sorafenib against HCT-116. The structure-activity relationship (SAR) analysis indicated that the indazole ring part tolerated different kinds of substituents, the N position of the central pyridine ring played key roles in antiproliferative activity...
March 24, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28338019/ghrelin-facilitates-glut2-sglt1-and-sglt2-mediated-intestinal-glucose-transport-in-goldfish-carassius-auratus
#6
Ayelén Melisa Blanco, Juan Ignacio Bertucci, Naresh Ramesh, María Jesús Delgado, Ana Isabel Valenciano, Suraj Unniappan
Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332558/multiple-h-sensors-mediate-the-extracellular-acidification-induced-ca-2-i-elevation-in-cultured-rat-ventricular-cardiomyocytes
#7
Yuan-Lang Hu, Xue Mi, Chao Huang, Hui-Fang Wang, Jian-Ren Song, Qing Shu, Lan Ni, Jian-Guo Chen, Fang Wang, Zhuang-Li Hu
Acidosis has been known to cause "Ca(2+) transients", however, the mechanism is still uncertain. Here, we demonstrated that multiple H(+) sensors, such as ASICs, TRPV1 and proton-sensing G protein coupled receptors (GPCRs) are involved in extracellular acidification-induced intracellular calcium ([Ca(2+)]i) elevation. By using calcium imaging measures, we observed that both ASIC and TRPV1 channels inhibitors suppressed the [Ca(2+)]i elevation induced by extracellular acidosis in cultured rat cardiac myocytes...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28332082/detection-of-terrain-indices-related-to-soil-salinity-and-mapping-salt-affected-soils-using-remote-sensing-and-geostatistical-techniques
#8
Hela Triki Fourati, Moncef Bouaziz, Mourad Benzina, Samir Bouaziz
Traditional surveying methods of soil properties over landscapes are dramatically cost and time-consuming. Thus, remote sensing is a proper choice for monitoring environmental problem. This research aims to study the effect of environmental factors on soil salinity and to map the spatial distribution of this salinity over the southern east part of Tunisia by means of remote sensing and geostatistical techniques. For this purpose, we used Advanced Spaceborne Thermal Emission and Reflection Radiometer data to depict geomorphological parameters: elevation, slope, plan curvature (PLC), profile curvature (PRC), and aspect...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28331821/comparison-between-endobronchial-ultrasound-guided-transbronchial-biopsy-and-ct-guided-transthoracic-lung-biopsy-for-the-diagnosis-of-peripheral-lung-cancer-a-systematic-review-and-meta-analysis
#9
Ping Zhan, Qing-Qing Zhu, Ying-Ying Miu, Ya-Fang Liu, Xiao-Xia Wang, Ze-Jun Zhou, Jia-Jia Jin, Qian Li, Shinji Sasada, Takehiro Izumo, Chih-Yen Tu, Wen-Chien Cheng, Matthew Evison, Tang-Feng Lv, Yong Song
BACKGROUND: With the release of the National Lung Screening Trial results, the detection of peripheral pulmonary lesions (PPLs) is likely to increase. Computed tomography (CT)-guided percutaneous transthoracic needle biopsy (PTNB) and radial probe endobronchial ultrasound (r-EBUS)-guided transbronchial lung biopsy (TBLB) are recommended for tissue diagnosis of PPLs. METHODS: A systematic review of published literature evaluating the accuracy of r-EBUS-TBLB and CT-PTNB for the diagnosis of PPLs was performed to determine point sensitivity and specificity, and to construct a summary receiver-operating characteristic curve...
February 2017: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/28331181/multi-walled-carbon-nanotubes-act-as-a-chemokine-and-recruit-macrophages-by-activating-the-plc-ip3-crac-channel-signaling-pathway
#10
Hui Li, Xiao-Qiu Tan, Li Yan, Bo Zeng, Jie Meng, Hai-Yan Xu, Ji-Min Cao
The impact of nanomaterials on immune cells is gaining attention but is not well documented. Here, we report a novel stimulating effect of carboxylated multi-walled carbon nanotubes (c-MWCNTs) on the migration of macrophages and uncover the underlying mechanisms, especially the upstream signaling, using a series of techniques including transwell migration assay, patch clamp, ELISA and confocal microscopy. c-MWCNTs dramatically stimulated the migration of RAW264.7 macrophages when endocytosed, and this effect was abolished by inhibiting phospholipase C (PLC) with U-73122, antagonizing the IP3 receptor with 2-APB, and blocking calcium release-activated calcium (CRAC) channels with SK&F96365...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28323939/tac1-gene-products-regulate-pituitary-hormone-secretion-and-gene-expression-in-prepubertal-grass-carp-pituitary-cells
#11
Guangfu Hu, Mulan He, Wendy K W Ko, Anderson O L Wong
Tachykinin 1 (TAC1) is known to have diverse functions in mammals but similar information is scarce in lower vertebrates including fish species. Using grass carp as a model, the pituitary actions, receptor specificity and post-receptor signaling of TAC1 gene products, namely substance P (SP) and neurokinin A (NKA), were examined. As a first step, TAC1 encoding carp SP and NKA as well as tachykinin receptors NK1R and NK2R were cloned in carp pituitary. Expression studies revealed that the newly cloned receptors were functional and with pharmacological properties similar to mammalian counterparts...
March 13, 2017: Endocrinology
https://www.readbyqxmd.com/read/28322461/iodinated-tg-in-thyroid-follicular-lumen-regulates-ttf-1-and-pax8-expression-via-tsh-tshr-signaling-pathway
#12
Huibin Huang, Yaxiong Shi, Bo Liang, Huiyao Cai, Qingyan Cai
Our previous study showed that highly iodinated thyroglobulin (TG) inhibited thyroid transcription factor-1 (TTF-1) and paired box gene 8 (PAX8) expression, but the potential mechanism remains unclear. In this study, we constructed a thyroid follicle model in vitro to mimic its natural physiological structure and explored how iodinated TG in the follicular lumen tuned TTF-1 and PAX8 expression. Our data showed that lowly iodinated TG enhanced PKA activity while upregulation of both TTF-1 and PAX8 expression; and that highly iodinated TG triggered PKC activity while suppression of TTF-1 and PAX8 expression...
March 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28315318/development-of-a-liquid-chromatography-mass-spectrometry-based-enzyme-activity-assay-for-phosphatidylcholine-specific-phospholipase-c
#13
Chiaki Murakami, Satoru Mizuno, Sayaka Kado, Fumio Sakane
Phosphatidylcholine (PC)-specific phospholipase C (PC-PLC) hydrolyzes PC to generate the second messenger 1,2-diacylglycerol (DG) and phosphocholine. PC-PLC plays pivotal roles in inflammation, carcinogenesis, tumor progression, atherogenesis, and subarachnoid hemorrhage. Although the activity of PC-PLC in mammalian tissues was discovered approximately 40 years ago, neither the protein nor its gene has been identified. In the present study, we developed a non-radioactive enzyme activity assay for PC-PLC based on mass spectrometric detection of DG following HPLC separation...
March 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28300784/the-molecular-basis-of-toxins-interactions-with-intracellular-signaling-via-discrete-portals
#14
REVIEW
Adi Lahiani, Ephraim Yavin, Philip Lazarovici
An understanding of the molecular mechanisms by which microbial, plant or animal-secreted toxins exert their action provides the most important element for assessment of human health risks and opens new insights into therapies addressing a plethora of pathologies, ranging from neurological disorders to cancer, using toxinomimetic agents. Recently, molecular and cellular biology dissecting tools have provided a wealth of information on the action of these diverse toxins, yet, an integrated framework to explain their selective toxicity is still lacking...
March 16, 2017: Toxins
https://www.readbyqxmd.com/read/28284560/computational-discovery-of-niclosamide-ethanolamine-a-repurposed-drug-candidate-that-reduces-growth-of-hepatocellular-carcinoma-cells-in-vitro-and-in-mice-by-inhibiting-cdc37-signaling
#15
Bin Chen, Wei Wei, Li Ma, Bin Yang, Ryan M Gill, Mei-Sze Chua, Atul J Butte, Samuel So
BACKGROUND & AIMS: Drug repositioning offers a shorter approval process than new drug development. We therefore searched large public datasets of drug-induced gene expression signatures to identify agents that might be effective against hepatocellular carcinoma (HCC). METHODS: We searched public databases of mRNA expression patterns reported from HCC specimens from patients, HCC cell lines, and cells exposed to various drugs. We identified drugs that might specifically increase expression of genes that are downregulated in HCCs and reduce expression of genes upregulated in HCCs using a non-parametric, rank-based pattern-matching strategy based on the Kolmogorov-Smirnov statistic...
March 8, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28276928/plectin-deficiency-in-liver-cancer-cells-promotes-cell-migration-and-sensitivity-to-sorafenib-treatment
#16
Chiung-Chi Cheng, Wei-Ting Chao, Chen-Chun Liao, Yu-Hui Tseng, Yen-Chang Clark Lai, Yih-Shyong Lai, Yung-Hsiang Hsu, Yi-Hsiang Liu
Plectin involved in activation of kinases in cell signaling pathway and plays important role in cell morphology and migration. Plectin knockdown promotes cell migration by activating focal adhesion kinase and Rac1-GTPase activity in liver cells. Sorafenib is a multi-targeting tyrosine kinase inhibitor that improves patient survival on hepatocellular carcinoma. The aim of this study is to investigate the correlation between the expression of plectin and cell migration as well as the sensitivity of hepatoma cell lines exposing to sorafenib...
February 17, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28273117/activation-of-odorant-receptor-in-colorectal-cancer-cells-leads-to-inhibition-of-cell-proliferation-and-apoptosis
#17
Lea Weber, Klaudia Al-Refae, Juliane Ebbert, Peter Jägers, Janine Altmüller, Christian Becker, Stephan Hahn, Günter Gisselmann, Hanns Hatt
The analysis and functional characterization of ectopically expressed human olfactory receptors (ORs) is becoming increasingly important, as many ORs have been identified in several healthy and cancerous tissues. OR activation has been demonstrated to have influence on cancer cell growth and progression. Here, ORs were identified using RNA-Seq analyses and RT-PCR. We demonstrated the OR protein localization in HCT116 cells using immunocytochemistry (IHC). In order to analyze the physiological role of OR51B4, we deorphanized the receptor by the use of CRE-Luciferase assays, conducted calcium imaging experiments as well as scratch- and proliferation assays...
2017: PloS One
https://www.readbyqxmd.com/read/28268001/return-to-duty-following-combat-related-multi-ligamentous-knee-injury
#18
Aaron E Barrow, Andrew J Sheean, Travis C Burns
INTRODUCTION: This retrospective cohort study characterized injury patterns, treatment practices, and identified the return to duty (RTD) rate following combat-related multi-ligament knee injuries (MLKI). PATIENTS AND METHODS: We evaluated injury characteristics and treatment methods of 46 military service members who had sustained a MLKI during combat activity. The primary clinical outcome measure was ability to return to active military duty. Secondary outcomes included subjective pain score, knee motion, knee instability, and use of ambulatory assistive device...
February 22, 2017: Injury
https://www.readbyqxmd.com/read/28266530/esr1-inhibits-hcg-induced-steroidogenesis-and-proliferation-of-progenitor-leydig-cells-in-mice
#19
Yeong Seok Oh, Il Kyoo Koh, Bomi Choi, Myung Chan Gye
Oestrogen is an important regulator in reproduction. To understand the role of oestrogen receptor 1 (ESR1) in Leydig cells, we investigated the expression of ESR1 in mouse Leydig cells during postnatal development and the effects of oestrogen on steroidogenesis and proliferation of progenitor Leydig cells (PLCs). In Leydig cells, the ESR1 expression was low at birth, increased until postnatal day 14 at which PLCs were predominant, and then decreased until adulthood. In foetal Leydig cells, ESR1 immunoreactivity increased from birth to postnatal day 14...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28265741/the-pleiotropic-vegetative-and-sexual-development-phenotypes-of-neurospora-crassa-arise-from-double-mutants-of-the-calcium-signaling-genes-plc-1-spla2-and-cpe-1
#20
Ananya Barman, Ranjan Tamuli
We investigated phenotypes of the double mutants of the calcium (Ca(2+)) signaling genes plc-1, splA2, and cpe-1 encoding for a phospholipase C1 (PLC-1), a secretory phospholipase A2 (sPLA2), and a Ca(2+)/H(+) exchanger (CPE-1), respectively, to understand the cell functions regulated by their genetic interactions. Mutants lacking plc-1 and either splA2 or cpe-1 exhibited numerous defects including reduced colonial growth, stunted aerial hyphae, premature conidiation on plates with delayed germination, inappropriate conidiation in submerged culture, and lesser mycelial pigmentation...
March 6, 2017: Current Genetics
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