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https://www.readbyqxmd.com/read/29776379/development-of-a-rapid-field-applicable-molecular-diagnostic-for-knockdown-resistance-kdr-markers-in-an-gambiae
#1
Vera T Unwin, Shaun Ainsworth, Emily J Rippon, El Hadji Amadou Niang, Mark J I Paine, David Weetman, Emily R Adams
BACKGROUND: The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics. METHODS: A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae...
May 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29769311/tumor-associated-calreticulin-variants-functionally-compromise-the-peptide-loading-complex-and-impair-its-recruitment-of-mhc-i
#2
Najla Arshad, Peter Cresswell
Major histocompatibility complex-I-β2 m dimers (MHC-I) bind peptides derived from intracellular proteins, enabling the immune system to distinguish between normal cells and those expressing pathogen-derived or mutant proteins. The peptides bind to MHC-I in the endoplasmic reticulum (ER), and this binding is facilitated by the peptide-loading complex (PLC), which contains calreticulin (CRT). CRT associates with MHC-I via a conserved glycan present on MHC-I and recruits it to the PLC for peptide binding. Somatic frameshift mutations in CRT (CRT-FS) drive the proliferation of a subset of myeloproliferative neoplasms (MPNs), which are chronic blood tumors...
May 16, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29766636/empagliflozin-as-add-on-to-linagliptin-in-a-fixed-dose-combination-in-japanese-patients-with-type-2-diabetes-glycemic-efficacy-and-safety-profile-in-a-52-week-randomized-placebo-controlled-trial
#3
Ryuzo Kawamori, Masakazu Haneda, Keiko Suzaki, Gang Cheng, Kosuke Shiki, Yuki Miyamoto, Fernando Solimando, Christopher Lee, Jisoo Lee, Jyothis George
AIMS: This double-blind, randomized, placebo-controlled trial evaluated the efficacy and safety of empagliflozin (Empa) 10 or 25 mg as add-on to linagliptin (Lina) 5 mg (fixed-dose combination, Empa/Lina 10/5 or 25/5) in insufficiently controlled Japanese type 2 diabetes patients. METHODS: The trial (40 sites; May 2015-March 2017) screened 433 adults (≥20 years) who were treatment-naive or on one oral antidiabetic drug for ≥12 weeks (discontinued at enrolment)...
May 15, 2018: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/29764501/eya4-inhibits-hepatocellular-carcinoma-growth-and-invasion-by-suppressing-nf-%C3%AE%C2%BAb-dependent-rap1-transactivation
#4
Shi-Jing Mo, Xun Hou, Xiao-Yi Hao, Jian-Peng Cai, Xin Liu, Wei Chen, Dong Chen, Xiao-Yu Yin
BACKGROUND: Our previous studies demonstrated that eyes absent homolog 4 (EYA4), a member of the eye development-related EYA family in Drosophila, is frequently methylated and silenced in hepatocellular carcinoma (HCC) specimens and associated with shorter survival. The current work aimed to explore the mechanisms through which EYA4 functions as a tumor suppressor in HCC. METHODS: Stable EYA4-expressing plasmid (pEYA4) transfectants of the human HCC cell lines Huh-7 and PLC/PRF/5 (PLC) were established...
April 3, 2018: Cancer communications
https://www.readbyqxmd.com/read/29758169/nutritional-recovery-from-low-protein-diet-during-pregnancy-does-not-restore-the-kinetics-of-insulin-secretion-and-ca2-or-alterations-in-the-camp-pka-and-plc-pkc-pathways-in-islets-from-adult-rats
#5
Leonardo Marin, Hellen Barbosa Farias Silva, Gabriela Damin, Leticia Martins Ignacio-Souza, Silvia Regina de Lima Reis, Camila Aparecida Machado de Oliveira, Rosane Aparecida Ribeiro, Marise A Reis, Marcia Queiroz Latorraca, Fabiano Ferreira, Vanessa Cristina Arantes
We investigated the insulin release induced by glucose, the Ca2+ oscillatory pattern and the cAMP/PKA and PLC/PKC pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling and after weaning and in rats receiving diets with 6% protein during gestation, followed by a 17% protein diet after birth (R). Glucose-induced first-phase insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify [Ca2+]i, but it reduced the [Ca2+] oscillatory frequency in the R group compared to the C group...
May 14, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29755690/prognostic-value-of-line-1-methylation-level-in-321-patients-with-primary-liver-cancer-including-hepatocellular-carcinoma-and-intrahepatic-cholangiocarcinoma
#6
Tatsunori Miyata, Yo-Ichi Yamashita, Yoshifumi Baba, Kazuto Harada, Takanobu Yamao, Naoki Umezaki, Masayo Tsukamoto, Yuki Kitano, Kensuke Yamamura, Kota Arima, Shigeki Nakagawa, Hirohisa Okabe, Katsunori Imai, Daisuke Hashimoto, Akira Chikamoto, Mototsugu Shimokawa, Hideo Baba
Background: The methylation level of long interspersed nucleotide element-1 (LINE-1) is a good surrogate marker of the global DNA methylation level. The relationship between LINE-1 methylation level and prognosis in primary liver cancer (PLC) patients remains unclear. Results: LINE-1 methylation levels were significantly lower in HCC and cHCC-CC tissues, but not in ICC tissues, than those in noncancerous liver parenchyma (HCC: p < 0.0001; cHCC-CC: p < 0.001; and ICC: p = 0...
April 17, 2018: Oncotarget
https://www.readbyqxmd.com/read/29750435/signaling-pathways-involved-in-human-sperm-hyperactivated-motility-stimulated-by-zn-2
#7
Deborah Allouche-Fitoussi, Danit Bakhshi, Haim Breitbart
To fertilize the egg, sperm cells must reside in the female reproductive tract for several hours during which they undergo chemical and motility changes collectively called capacitation. During capacitation, the sperm develop a unique type of motility known as hyper-activated motility (HAM). The semen contains Zn2+ in millimolar concentrations, whereas in the female reproductive tract the concentration is around 1 µM. In this study, we characterize the role of Zn2+ in human sperm capacitation focusing on its effect on HAM...
May 11, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29750009/xen-glaucoma-treatment-system-in-the-management-of-refractory-glaucomas-a-short-review-on-trial-data-and-potential-role-in-clinical-practice
#8
REVIEW
A De Gregorio, E Pedrotti, G Stevan, A Bertoncello, S Morselli
The recent development of new devices that are significantly less invasive, collectively termed minimally invasive glaucoma surgery, offers new perspective of intraocular pressure reduction with less risk, short operating times, and rapid recovery. The aim of this work is to provide a panoramic review of the currently published clinical data to assess the potential role of XEN gel stent (Allergan PLC, Irvine, CA, USA) in the management of glaucoma, which is the only filtering minimally invasive glaucoma surgery device that allows the subconjunctival filtration...
2018: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29749382/microrna-501-3p-suppresses-metastasis-and-progression-of-hepatocellular-carcinoma-through-targeting-lin7a
#9
Chubin Luo, Dan Yin, Hao Zhan, Uyunbilig Borjigin, Chuanjiang Li, Zhengjun Zhou, Zhiqiang Hu, Pengcheng Wang, Qiman Sun, Jia Fan, Jian Zhou, Xin Wang, Shaolai Zhou, Xiaowu Huang
Increasing numbers of evidences have demonstrated that microRNAs (miRNAs) are implicated in metastasis and progression of hepatocellular carcinoma (HCC). However, their detailed expression levels and actual functions in HCCs have not been fully clarified yet. Results from our recent study revealed that some miRNAs were particularly related to metastasis of HCCs. As one of these newly found miRNAs, miR-501-3p showed to highly involve into metastatic process of HCCs. Here we reported that the expression of miR-501-3p was decreased in both metastatic HCC cell lines and tissue samples from HCC patients with recurrence and metastasis...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29748913/efficacy-and-tolerability-of-fixed-dose-combination-perindopril-indapamide-in-hypertensive-patients-with-a-history-of-stroke-or-transient-ischemic-attack-picasso-trial
#10
Csaba András Dézsi, Csaba Farsang
INTRODUCTION: In everyday medical practice, physicians often need to manage patients whose blood pressure is not well controlled. Those with a history of cerebrovascular disease are a high-risk group in need of rapid blood pressure control. METHODS: The PICASSO study was a real-life, observational trial involving 9257 inadequately treated hypertensive patients who were switched from previous therapy to the fixed-dose combination of perindopril 10 mg/indapamide 2...
May 10, 2018: Advances in Therapy
https://www.readbyqxmd.com/read/29748614/nanovesicle-mediated-delivery-of-anticancer-agents-effectively-induced-cell-death-and-regressed-intrahepatic-tumors-in-athymic-mice
#11
Joseph George, Irene K Yan, Tushar Patel
Hepatocellular carcinoma is highly resistant to chemotherapy. Here we evaluated the use and efficacy of milk-derived nanovesicles (MNV) as an approach to improve delivery of anticancer agents into HCC cells and intrahepatic tumors. We developed a protocol for isolation of MNVs from skim milk using ultracentrifugation, and characterized using nanoparticle tracking analysis (NTA) and electron microscopy. MNVs were loaded with doxorubicin (dox-MNV) or miR221 antisense oligonucleotides (anti-miR221-MNV), and further evaluated using spectrophotometry, NTA, and zeta potential measurements...
May 10, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29746909/characterization-of-lpxrfa-receptor-in-the-half-smooth-tongue-sole-cynoglossus-semilaevis-molecular-cloning-expression-profiles-and-differential-activation-of-signaling-pathways-by-lpxrfa-peptides
#12
Bin Wang, Guokun Yang, Quan Liu, Jingkai Qin, Yongjiang Xu, Wensheng Li, Xuezhou Liu, Bao Shi
Gonadotropin-inhibitory hormone (GnIH), a novel hypothalamic neuropeptide, serves as a key player in the regulation of reproduction across vertebrates, acting on the brain and pituitary to modulate reproductive physiology and behavior. However, little information is available in teleosts regarding the intracellular signal transduction pathway in response to GnIH. To this end, we first cloned the gene of LPXRFa (the piscine ortholog of GnIH) receptor in the half-smooth tongue sole (Cynoglossus semilaevis), a representative species of the order Pleuronectiformes...
May 7, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29745758/evaluation-of-heating-conditions-for-inactivation-of-hepatitis-e-virus-genotypes-3-and-4
#13
Toshifumi Imagawa, Ryuichi Sugiyama, Tomoyuki Shiota, Tian-Cheng Li, Sayaka Yoshizaki, Takaji Wakita, Koji Ishii
Hepatitis E virus (HEV) is a causative agent of acute hepatitis throughout the world. HEV genotypes 1 through 4 infect humans, whereas genotypes 3 and 4 (Gt3 and Gt4) also infect other animals. In developed countries, the main HEV infection route is by foodborne transmission, resulting from the consumption of undercooked meat. It is important to know the criteria for HEV control in daily cooking. In this study, we assessed the heat conditions required to inactivate HEV Gt3 and Gt4 in culture supernatants and spiked minced pork meat...
May 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29743977/formyl-peptide-receptor-1-modulates-endothelial-cell-functions-by-nadph-oxidase-dependent-vegfr2-transactivation
#14
Fabio Cattaneo, Martina Castaldo, Melania Parisi, Raffaella Faraonio, Gabriella Esposito, Rosario Ammendola
In the vasculature, NADPH oxidase is the main contributor of reactive oxygen species (ROS) which play a key role in endothelial signalling and functions. We demonstrate that ECV304 cells express p47phox , p67phox , and p22phox subunits of NADPH oxidase, as well as formyl peptide receptors 1 and 3 (FPR1/3), which are members of the GPCR family. By RT-PCR, we also detected Flt-1 and Flk-1/KDR in these cells. Stimulation of FPR1 by N-fMLP induces p47phox phosphorylation, which is the crucial event for NADPH oxidase-dependent superoxide production...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29743498/calretinin-participates-in-regulating-steroidogenesis-by-plc-ca-2-pkc-pathway-in-leydig-cells
#15
Wendan Xu, Qian Zhu, Shan Liu, Xiaonan Dai, Bei Zhang, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui
Calretinin, a Ca2+ -binding protein, participates in many cellular events. Our previous studies found the high expression of calretinin in testicular Leydig cells. In this study, (MLTC-1 cells were infected with LV-calb2, R2C cells with LV-siRNA-calb2. The primary mouse Leydig cells were also used to confirm those data from cell lines. Testosterone level was significantly higher in the MLTC-1 cells with over-expressed calretinin than in the control, while progesterone was lower in the R2C cells in which down-regulated calretinin...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29740015/effects-of-5-aminolevulinic-acid-supplementation-on-home-based-walking-training-achievement-in-middle-aged-depressive-women-randomized-double-blind-crossover-pilot-study
#16
Hiroshi Suzuki, Shizue Masuki, Akiyo Morikawa, Yu Ogawa, Yoshi-Ichiro Kamijo, Kiwamu Takahashi, Motowo Nakajima, Hiroshi Nose
Depressive patients often experience difficulty in performing exercise due to physical and psychological barriers. We examined the effects of 5-aminolevulinic acid (ALA) with sodium ferrous citrate (SFC) supplementation during home-based walking training in middle-aged depressive women. Nine outpatients [53 ± 8 (SD) yr] with major depressive disorder participated in the pilot study with randomized, placebo-controlled, double-blind crossover design. They underwent two trials for 7 days, each performing interval walking training (IWT) with ALA + SFC (ALA + SFC) or placebo supplement intake (PLC) intermittently with >a 10-day washout period...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29738822/the-drosophila-light-activated-trp-and-trpl-channels-targets-of-the-phosphoinositide-signaling-cascade
#17
REVIEW
Ben Katz, Baruch Minke
The Drosophila light-activated Transient Receptor Potential (TRP) channel is the founding member of a large and diverse family of channel proteins. It is now established that TRP channels are evolutionarily conserved and are found in many organisms and tissues. This review outlines the progress made in our understanding of Drosophila phototransduction with a focus on the light sensitive TRP channels. The visual system of Drosophila has remarkable capabilities, such as single photon sensitivity, low dark noise, wide dynamic range of responses to changing ambient light intensities and an unusually wide range of frequency responses to modulated lights...
May 5, 2018: Progress in Retinal and Eye Research
https://www.readbyqxmd.com/read/29733511/lenvatinib-inhibits-angiogenesis-and-tumor-fibroblast-growth-factor-signaling-pathways-in-human-hepatocellular-carcinoma-models
#18
Masahiro Matsuki, Taisuke Hoshi, Yuji Yamamoto, Megumi Ikemori-Kawada, Yukinori Minoshima, Yasuhiro Funahashi, Junji Matsui
Unresectable hepatocellular carcinoma (uHCC) is one of the most lethal and prevalent cancers worldwide, and current systemic therapeutic options for uHCC are limited. Lenvatinib, a multiple receptor tyrosine kinase inhibitor targeting vascular endothelial growth factor receptors (VEGFRs) and fibroblast growth factor receptors (FGFRs), recently demonstrated a treatment effect on overall survival by statistical confirmation of noninferiority to sorafenib in a phase 3 study of uHCC. Here, we investigated mechanisms underlying the antitumor activity of lenvatinib in preclinical HCC models...
May 7, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29730398/capa-periviscerokinin-mediated-activation-of-mapk-erk-signaling-through-gq-plc-pkc-dependent-cascade-and-reciprocal-erk-activation-dependent-internalized-kinetics-of-bom-capa-pvk-receptor-2
#19
Zhangfei Shen, Xiaoyuan Yang, Yu Chen, Liangen Shi
Bombyx mori neuropeptide G protein-coupled receptor (BNGR)-A27 is a specific receptor for B. mori capability (CAPA) periviscerokinin (PVK), that is, Bom-CAPA-PVK receptor 2. Upon stimulation of Bom-CAPA-PVK-1 or -PVK-2, Bom-CAPA-PVK receptor 2 significantly increases cAMP-response element-controlled luciferase activity and Ca2+ mobilization in a Gq inhibitor-sensitive manner. However, the underlying mechanism(s) for CAPA/CAPA receptor system mediation of extracellular signal-regulated kinases1/2 (ERK1/2) activation remains to be explained further...
May 3, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29728247/mining-protein-phosphorylation-information-from-biomedical-literature-using-nlp-parsing-and-support-vector-machines
#20
Kalpana Raja, Jeyakumar Natarajan
BACKGROUND: Extraction of protein phosphorylation information from biomedical literature has gained much attention because of the importance in numerous biological processes. OBJECTIVE: In this study, we propose a text mining methodology which consists of two phases, NLP parsing and SVM classification to extract phosphorylation information from literature. METHODS: First, using NLP parsing we divide the data into three base-forms depending on the biomedical entities related to phosphorylation and further classify into ten sub-forms based on their distribution with phosphorylation keyword...
July 2018: Computer Methods and Programs in Biomedicine
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