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https://www.readbyqxmd.com/read/28916718/potential-sperm-contributions-to-the-murine-zygote-predicted-by-in-silico-analysis
#1
Panagiotis Ntostis, Deborah Carter, David Iles, J D Huntriss, Maria Tzetis, David Miller
Paternal contributions to the zygote are thought to extend beyond delivery of the genome and paternal RNAs have been linked to epigenetic transgenerational inheritance in different species. In addition, sperm-egg fusion activates several downstream processes that contribute to zygote formation, including PLC zeta-mediated egg activation and maternal RNA clearance. Since a third of the preimplantation developmental period in the mouse occurs prior to the first cleavage stage, there is ample time for paternal RNAs or their encoded proteins potentially to interact and participate in early zygotic activities...
September 15, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28911184/subcellular-localization-and-function-study-of-a-secreted-phospholipase-c-from-nocardia-seriolae
#2
Liqun Xia, Haiying Liang, Liang Xu, Jianlin Chen, Michaël Bekaert, Honglian Zhang, Yishan Lu
Fish nocardiosis is a chronic systemic granulomatous disease, and Nocardia seriolae is the main pathogen that causes it. The pathogenesis and virulence factors of N. seriolae are not fully understood. A phospholipase C (PLC), which is likely to be a secreted protein targeting host cell mitochondria, was found by a bioinformatics analysis of the whole genome sequence of N. seriolae. In order to determine the subcellular localization and study the preliminary function of PLC from N. seriolae (NsPLC), in this study gene cloning, secreted protein identification, subcellular localization in host cells and apoptosis detection of NsPLC were carried out...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28910987/management-of-combined-injuries-of-the-posterior-cruciate-ligament-and-posterolateral-corner-of-the-knee-a-systematic-review
#3
Stefano Petrillo, Piero Volpi, Rocco Papalia, Nicola Maffulli, Vincenzo Denaro
Background: Approximately 60% of posterior cruciate ligament (PCL) injury are associated with a posterolateral corner (PLC) tear. Sources of data: We performed a systematic review of the literature according to the PRISMA guidelines. The following key words were searched on Medline, Cochrane, EMBASE, Google Scholar, and Ovid: 'posterior cruciate ligament' or 'PCL' with 'posterolateral corner' or 'PLC' and 'chronic'; 'injury'; 'management'; 'reconstruction'; 'outcomes'; 'complications'...
September 1, 2017: British Medical Bulletin
https://www.readbyqxmd.com/read/28910200/comparison-of-two-%C3%AE-alanine-dosing-protocols-on-muscle-carnosine-elevations
#4
David D Church, Jay R Hoffman, Alyssa N Varanoske, Ran Wang, Kayla M Baker, Michael B La Monica, Kyle S Beyer, Sarah J Dodd, Leonardo P Oliveira, Roger C Harris, David H Fukuda, Jeffrey R Stout
OBJECTIVE: β-alanine (BA) is a nonproteogenic amino acid that combines with histidine to form carnosine. The amount taken orally in individual doses, however, is limited due to symptoms of paresthesia that are associated with higher doses. The use of a sustained-release formulation has been reported to reduce the symptoms of paresthesia, suggesting that a greater daily dose may be possible. The purpose of the present study was to determine whether increasing the daily dose of BA can result in a similar increase in muscle carnosine in a reduced time...
September 14, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28904864/a-g-protein-coupled-%C3%AE-7-nicotinic-receptor-regulates-signaling-and-tnf-%C3%AE-release-in-microglia
#5
Justin R King, Trudy C Gillevet, Nadine Kabbani
Acetylcholine activation of α7 nicotinic acetylcholine receptors (α7 nAChRs) in microglia attenuates neuroinflammation and regulates TNF-α release. We used lipopolysaccharide to model inflammation in the microglial cell line EOC20 and examined signaling by the α7 nAChR. Co-immunoprecipitation experiments confirm that α7 nAChRs bind heterotrimeric G proteins in EOC20 cells. Interaction with Gαi mediates α7 nAChR signaling via enhanced intracellular calcium release and a decrease in cAMP, p38 phosphorylation, and TNF-α release...
September 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28904023/%C3%AE-linolenic-acid-derived-metabolites-from-gut-lactic-acid-bacteria-induce-differentiation-of-anti-inflammatory-m2-macrophages-through-g-protein-coupled-receptor-40
#6
Ryuji Ohue-Kitano, Yumiko Yasuoka, Tsuyoshi Goto, Nahoko Kitamura, Si-Bum Park, Shigenobu Kishino, Ikuo Kimura, Mayu Kasubuchi, Haruya Takahashi, Yongjia Li, Yu-Sheng Yeh, Huei-Fen Jheng, Mari Iwase, Masashi Tanaka, Shinya Masuda, Takayuki Inoue, Hajime Yamakage, Toru Kusakabe, Fumito Tani, Akira Shimatsu, Nobuyuki Takahashi, Jun Ogawa, Noriko Satoh-Asahara, Teruo Kawada
Among dietary fatty acids with immunologic effects, ω-3 polyunsaturated fatty acids, such as α-linolenic acid (ALA), have been considered as factors that contribute to the differentiation of M2-type macrophages (M2 macrophages). In this study, we examined the effect of ALA and its gut lactic acid bacteria metabolites 13-hydroxy-9(Z),15(Z)-octadecadienoic acid (13-OH) and 13-oxo-9(Z),15(Z)-octadecadienoic acid (13-oxo) on the differentiation of M2 macrophages from bone marrow-derived cells (BMDCs) and investigated the underlying mechanisms...
September 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28903399/phosphatidylcholine-specific-phospholipase-c-inhibition-reduces-her2-overexpression-cell-proliferation-and-in-vivo-tumor-growth-in-a-highly-tumorigenic-ovarian-cancer-model
#7
Luisa Paris, Franca Podo, Francesca Spadaro, Laura Abalsamo, Maria Elena Pisanu, Alessandro Ricci, Serena Cecchetti, Luisa Altabella, Maria Buoncervello, Ludmila Lozneanu, Marina Bagnoli, Carlo Ramoni, Silvana Canevari, Delia Mezzanzanica, Egidio Iorio, Rossella Canese
Antagonizing the oncogenic effects of human epidermal growth factor receptor 2 (HER2) with current anti-HER2 agents has not yet yielded major progress in the treatment of advanced HER2-positive epithelial ovarian cancer (EOC). Using preclinical models to explore alternative molecular mechanisms affecting HER2 overexpression and oncogenicity may lead to new strategies for EOC patient treatment. We previously reported that phosphatidylcholine-specific phospholipase C (PC-PLC) exerts a pivotal role in regulating HER2 overexpression in breast cancer cells...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28892824/resolvin-d1-increases-mucin-secretion-in-cultured-rat-conjunctival-goblet-cells-via-multiple-signaling-pathways
#8
Marit Lippestad, Robin R Hodges, Tor P Utheim, Charles N Serhan, Darlene A Dartt
Purpose: Goblet cells in the conjunctiva secrete mucin into the tear film protecting the ocular surface. The proresolution mediator resolvin D1 (RvD1) regulates mucin secretion to maintain homeostasis during physiological conditions and in addition, actively terminates inflammation. We determined the signaling mechanisms used by RvD1 in cultured rat conjunctival goblet cells to increase intracellular [Ca2+] ([Ca2+]i) and induce glycoconjugate secretion. Methods: Increase in [Ca2+]i were measured using fura 2/AM and glycoconjugate secretion determined using an enzyme-linked lectin assay with the lectin Ulex Europaeus Agglutinin 1...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28882164/the-role-of-phospholipase-c-signaling-in-bovine-herpesvirus-1-infection
#9
Liqian Zhu, Chen Yuan, Xiuyan Ding, Clinton Jones, Guoqiang Zhu
Bovine herpesvirus 1 (BoHV-1) infection enhanced the generation of inflammatory mediator reactive oxidative species (ROS) and stimulated MAPK signaling that are highly possibly related to virus induced inflammation. In this study, for the first time we show that BoHV-1 infection manipulated phospholipase C (PLC) signaling, as demonstrated by the activation of PLCγ-1 at both early stages [at 0.5 h post-infection (hpi)] and late stages (4-12 hpi) during the virus infection of MDBK cells. Viral entry, and de novo protein expression and/or DNA replication were potentially responsible for the activation of PLCγ-1 signaling...
September 7, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28878075/the-characterization-of-the-quasi-enveloped-hepatitis-e-virus-particles-released-by-the-cellular-exosomal-pathway
#10
Shigeo Nagashima, Masaharu Takahashi, Tominari Kobayashi, Tanggis, Tsutomu Nishizawa, Takashi Nishiyama, Putu Prathiwi Primadharsini, Hiroaki Okamoto
Our previous studies demonstrated that membrane-associated hepatitis E virus (HEV) particles-now considered "quasi-enveloped particles"-are present in the multivesicular body with intraluminal vesicles (exosomes) in infected cells, and that the release of HEV virions is related to the exosomal pathway. In this study, we characterized exosomes purified from culture supernatant of HEV-infected PLC/PRF/5 cells. Purified CD63-, CD9-, or CD81-positive exosomes derived from the culture supernatant of HEV-infected cells that had been cultivated in serum-free medium were found to contain HEV RNA and the viral capsid (ORF2) and ORF3 proteins, as determined by a RT-PCR and Western blotting, respectively...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28877214/direct-versus-indirect-actions-of-ghrelin-on-hypothalamic-npy-neurons
#11
Hiroshi Hashiguchi, Zhenyu Sheng, Vanessa Routh, Volodymyr Gerzanich, J Marc Simard, Joseph Bryan
OBJECTIVES: Assess direct versus indirect action(s) of ghrelin on hypothalamic NPY neurons. MATERIALS AND METHODS: Electrophysiology was used to measure ion channel activity in NPY-GFP neurons in slice preparations. Ca2+ imaging was used to monitor ghrelin activation of isolated NPY GFP-labeled neurons. Immunohistochemistry was used to localize Trpm4, SUR1 and Kir6.2 in the hypothalamus. RESULTS: Acylated ghrelin depolarized the membrane potential (MP) of NPY-GFP neurons in brain slices...
2017: PloS One
https://www.readbyqxmd.com/read/28875842/targeting-cxcl12-cxcr4-axis-in-tumor-immunotherapy
#12
Weiqiang Zhou, Shanchun Guo, Mingli Liu, Matthew E Burow, Guangdi Wang
Chemokines, which have chemotactic abilities, are comprised of over 50 family members. Through binding to the 7-transmembrane domain of G-protein-coupled receptors (GPCR), they function in immune cells by trafficking and regulating cell prolif¬eration, differentiation, activation, and migration, homing under both physiologic and pathologic conditions. The alpha-chemokine receptor CXCR4 for the alpha-chemokine stromal cell-derived-factor-1 (SDF-1) is most widely expressed by tumors. CXCL12/CXCR4 axis is a major culprit for human tumor because of its crucial role in tumor initiation and progression by activating a number of signaling pathways, such as ERK1/2, ras, PLC/ MAPK, p38 MAPK, and SAPK/ JNK, as well as regulating cancer stem cells...
August 29, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28873320/influence-of-rhodiola-rosea-on-the-heat-acclimation-process-in-young-healthy-men
#13
Saima Timpmann, Anthony C Hackney, Maria Tamm, Kairi Kreegipuu, Eve Unt, Vahur Ööpik
The adaptogen <i>Rhodiola rosea</i> (RR) may mitigate stress responses and have beneficial effects on endurance capacity (EC) and mental performance. Heat acclimation (HA) improves EC in the heat, but the potential impact of RR on the HA process is unknown. Therefore, our intent was to determine would RR impact positively on the efficacy of the HA process. Twenty male subjects (age 22.5 ± 3.0 years) completed two EC tests involving walking (6 km·h <sup>-1</sup>) until volitional exhaustion in a climate chamber (air temperature, 42°C; relative humidity, 18%) before (H1) and after (H2) an 8-day HA period...
September 5, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28873085/the-association-between-fasting-blood-glucose-and-the-risk-of-primary-liver-cancer-in-chinese-males-a-population-based-prospective-study
#14
Xiaoshuang Feng, Gang Wang, Ni Li, Zhangyan Lyu, Shuohua Chen, Luopei Wei, Yuheng Chen, Shuanghua Xie, Wenjing Yang, Jian Yin, Hong Cui, Hongda Chen, Jiansong Ren, Jufang Shi, Shouling Wu, Min Dai, Jie He
BACKGROUND: To investigate the association between fasting blood glucose (FBG) levels and the risk of incident primary liver cancer (PLC) in Chinese males, a large prospective cohort was performed in the current study. METHODS: A total of 109 169 males participating in the routine checkups every two years were recruited in the Kailuan male cohort study since May 2006. Cox proportional hazards regression models and restricted cubic spline (RCS) were used to evaluate the association between levels of baseline FBG and the risk of incident PLC...
September 5, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28870323/poly-l-cysteine-electrospun-copper-oxide-nanofibers-zinc-oxide-nanoparticles-nanocomposite-as-sensing-element-of-an-electrochemical-sensor-for-simultaneous-determination-of-adenine-and-guanine-in-biological-samples-and-evaluation-of-damage-to-dsdna-and-dna
#15
Majid Arvand, Masoomeh Sayyar Ardaki
A new nanocomposite film constructed of poly-l-cysteine/zinc oxide nanoparticles-electrospun copper oxide nanofibers (PLC/ZnO-NPs-CuO-NFs) was prepared on the surface of the graphite electrode (GE). The novel electrode was successfully applied for the simultaneous determination of guanine (G) and adenine (A), two of the most important components of DNA and RNA. The PLC/ZnO-NPs-CuO-NFs/GE enhanced the anodic peak currents of the purine bases conspicuously and could determine them sensitively and separately in 0...
September 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28870198/up-modulation-of-plc-%C3%AE-2-reduces-the-number-and-malignancy-of-triple-negative-breast-tumor-cells-with-a-cd133-epcam-phenotype-a-promising-target-for-preventing-progression-of-tnbc
#16
Federica Brugnoli, Silvia Grassilli, Paola Lanuti, Marco Marchisio, Yasamin Al-Qassab, Federica Vezzali, Silvano Capitani, Valeria Bertagnolo
BACKGROUND: The malignant potential of triple negative breast cancer (TNBC) is also dependent on a sub-population of cells with a stem-like phenotype. Among the cancer stem cell markers, CD133 and EpCAM strongly correlate with breast tumor aggressiveness, suggesting that simultaneous targeting of the two surface antigens may be beneficial in treatment of TNBC. Since in TNBC-derived cells we demonstrated that PLC-β2 induces the conversion of CD133(high) to CD133(low) cells, here we explored its possible role in down-modulating the expression of both CD133 and EpCAM and, ultimately, in reducing the number of TNBC cells with a stem-like phenotype...
September 4, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28863818/depletion-of-membrane-cholesterol-suppresses-drosophila-transient-receptor-potential-like-trpl-channel-activity
#17
Maximilian Peters, Ben Katz, Shaya Lev, Rachel Zaguri, Rita Gutorov, Baruch Minke
Cholesterol is an essential compound of higher eukaryotic cell membranes and a known modulator of ion channel activity. Changes in phospholipids and cholesterol composition of cell membranes are known to alter the activity of ion channels. However, there is little knowledge on the effects of cholesterol on transient receptor potential (TRP) channels. In this study, we explore the effects of cholesterol depletion on the Drosophila photoreceptor channel TRP-like (TRPL), when expressed in tissue culture cells...
2017: Current Topics in Membranes
https://www.readbyqxmd.com/read/28862959/hypo-attenuated-leaflet-thickening-and-reduced-leaflet-motion-in-sutureless-bioprosthetic-aortic-valves
#18
Magnus Dalén, Ulrik Sartipy, Kerstin Cederlund, Anders Franco-Cereceda, Anders Svensson, Raquel Themudo, Peter Svenarud, Elin Bacsovics Brolin
BACKGROUND: The prevalence of hypo-attenuated leaflet thickening (HALT) and reduced leaflet motion (RLM) is unknown in surgically implanted bioprostheses because systematic investigation of HALT and/or RLM is limited to a few catheter-based valves. The aim of this study was to investigate the prevalence of HALT and RLM by cardiac computed tomography in patients who underwent surgical aortic valve replacement and received a Perceval sutureless aortic valve bioprosthesis. METHODS AND RESULTS: This was a single-center prospective observational study that included 47 patients (83...
August 21, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28859220/projections-of-primary-liver-cancer-to-2030-in-30-countries-worldwide
#19
Patricia C Valery, Mathieu Laversanne, Paul J Clark, Jessica L Petrick, Katherine A McGlynn, Freddie Bray
Primary liver cancer (PLC) is the sixth most common cancer worldwide and the second most common cause of cancer death. Future predictions can inform health planners and raise awareness of the need for cancer control action. We predicted the future burden of PLC in 30 countries around 2030. Incident cases of PLC (ICD-10 C22) were obtained from 30 countries for 1993-2007. We projected new PLC cases through to 2030 using age-period-cohort models (NORDPRED). Age-standardized incidence rates per 100,000 person-years were calculated by country and sex...
August 31, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28858926/a-prospective-open-label-study-of-hyaluronic-acid-based-filler-with-lidocaine-vyc-15l-treatment-for-the-correction-of-infraorbital-skin-depressions
#20
Francois Niforos, Raj Acquilla, Patricia Ogilvie, Marva Safa, Massimo Signorini, Lela Creutz, Graeme Kerson, Michael Silberberg
BACKGROUND: Infraorbital skin depressions are one of the most troublesome facial areas for aesthetically aware patients. OBJECTIVE: Evaluate effectiveness and safety of Juvéderm Volbella with Lidocaine (VYC-15L; Allergan plc, Dublin, Ireland) for correction of bilateral infraorbital depressions. METHODS: In this 12-month, prospective, uncontrolled, open-label study, subjects aged ≥18 years with infraorbital depressions rated ≥1 on the Allergan Infra-oRbital Scale (AIRS) received injections of VYC-15L with optional touch-up treatment on Day 14...
August 30, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
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