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https://www.readbyqxmd.com/read/27809990/the-roles-of-immune-cells-in-bone-healing-what-we-know-do-not-know-and-future-perspectives
#1
REVIEW
Jehan J El-Jawhari, Elena Jones, Peter V Giannoudis
Key events occurring during the bone healing include well-orchestrated and complex interactions between immune cells, multipotential stromal cells (MSCs), osteoblasts and osteoclasts. Through three overlapping phases of this physiological process, innate and adaptive immune cells, cytokines and chemokines have a significant role to play. The aim of the escalating immune response is to achieve an osseous healing in the shortest time and with the least complications facilitating the restoration of function. The uninterrupted progression of these biological events in conjunction with a favourable mechanical environment (stable fracture fixation) remains the hallmark of successful fracture healing...
November 2016: Injury
https://www.readbyqxmd.com/read/27797908/hematopoietic-stem-cell-mobilization-is-necessary-but-not-sufficient-for-tolerance-in-islet-transplantation
#2
Blair T Stocks, Analise B Thomas, Sydney K Elizer, Yuantee Zhu, Andrew F Marshall, Christopher S Wilson, Daniel J Moore
Overcoming the immune response to establish durable immune tolerance in Type 1 Diabetes remains a substantial challenge. The ongoing effector immune response involves numerous immune cell types but is ultimately orchestrated and sustained by the hematopoietic stem cell (HSC) niche. We therefore hypothesized that tolerance induction also requires these pluripotent precursors. Herein, we determined that the tolerance inducing agent anti-CD45RB induces HSC mobilization in non-autoimmune B6 mice but not in diabetes-prone NOD mice...
October 26, 2016: Diabetes
https://www.readbyqxmd.com/read/27787807/in-vitro-comparison-of-the-efficacy-of-tgf-%C3%AE-1-and-pdgf-bb-in-combination-with-freeze-dried-bone-allografts-for-induction-of-osteogenic-differentiation-in-mg-63-osteoblast-like-cells
#3
Surena Vahabi, Maryam Torshabi, Azadeh Esmaeil Nejad
Predictable regeneration of alveolar bone defects has always been a challenge in implant dentistry. Bone allografts are widely used bone substitutes with controversial osteoinductive activity. This in vitro study aimed to assess the osteogenic potential of some commercially available freeze-dried bone allografts supplemented with human recombinant platelet-derived growth factor-BB and transforming growth factor beta-1. Cell viability, mineralization, and osteogenic gene expression of MG-63 osteoblast-like cells were compared among the allograft alone, allograft/platelet-derived growth factor-BB, allograft/transforming growth factor beta-1, and allograft/platelet-derived growth factor-BB/transforming growth factor beta-1 groups...
December 2016: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27771827/recombinant-human-bone-morphogenetic-protein-rhbmp-9-induces-osteoblast-differentiation-when-combined-with-demineralized-freeze-dried-bone-allografts-dfdbas-or-biphasic-calcium-phosphate-bcp
#4
Masako Fujioka-Kobayashi, Benoit Schaller, Yufeng Zhang, Umadevi Kandalam, Maria Hernandez, Richard J Miron
OBJECTIVES: Recently, recombinant human bone morphogenetic protein 9 (rhBMP9) has been characterized as one of the most osteogenic growth factors among the 15 human BMPs. The aim of the present study was to investigate the effects of rhBMP9 in comparison to the clinically utilized rhBMP2 on in vitro cell behavior when combined with two bone graft materials including demineralized freeze-dried bone allografts (DFDBAs) and biphasic calcium phosphate (BCP). MATERIALS AND METHODS: The absorption and release kinetics of rhBMPs from DFDBA and BCP were investigated by ELISA...
October 22, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27560507/response-of-dental-pulp-stem-cells-to-synthetic-allograft-and-xenograft-bone-scaffolds
#5
Saeed Reza Motamedian, Fahimeh Sadat Tabatabaei, Fahimeh Akhlaghi, Maryam Torshabi, Parisan Gholamin, Arash Khojasteh
Different degrees of clinical success have been reported for synthetic, allograft, and xenograft bone substitutes in human trials. Although these substitutes have been clinically investigated, their in vitro effects on cell differentiation remain unclear. Proliferation, differentiation, and attachment of dental pulp stem cells (DPSCs) to β-tricalcium phosphate (β-TCP), freeze-dried bone allograft (FDBA), and deproteinized bovine bone mineral (DBBM) were compared in this study. MTT assay, measurement of total DNA, and reverse transcriptase polymerase chain reaction were performed...
August 24, 2016: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/27529782/cellular-vascular-and-histomorphometric-outcomes-of-solvent-dehydrated-vs-freeze-dried-allogeneic-graft-for-maxillary-sinus-augmentation-a-randomized-case-series
#6
Alberto Monje, Francisco O'Valle, Florencio Monje-Gil, Inmaculada Ortega-Oller, Francisco Mesa, Hom-Lay Wang, Pablo Galindo-Moreno
PURPOSE: The aim of this study was to compare solvent dehydrated human allograft (SDHA; Puros Allograft) and freeze-dried human allograft (FDHA; MinerOss) in order to determine if the allogeneic bone preservation process influences the amount of remaining particles and newly formed bone in maxillary sinus augmentation. MATERIALS AND METHODS: Subjects requiring maxillary lateral sinus augmentation with ridge height < 5 mm were included in this study. Maxillary sinuses were randomly assigned to be grafted with a 1:1 ratio of cortical and cancellous bone, either SDHA or FDHA...
August 15, 2016: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/27446265/use-of-a-biological-reactor-and-platelet-rich-plasma-for-the-construction-of-tissue-engineered-bone-to-repair-articular-cartilage-defects
#7
Huibo Li, Shui Sun, Haili Liu, Hua Chen, Xin Rong, Jigang Lou, Yunbei Yang, Yi Yang, Hao Liu
Articular cartilage defects are a major clinical burden worldwide. Current methods to repair bone defects include bone autografts, allografts and external fixation. In recent years, the repair of bone defects by tissue engineering has emerged as a promising approach. The present study aimed to assess a novel method using a biological reactor with platelet-rich plasma to construct tissue-engineered bone. Beagle bone marrow mesenchymal stem cells (BMSCs) were isolated and differentiated into osteoblasts and chondroblasts using platelet-rich plasma and tricalcium phosphate scaffolds cultured in a bioreactor for 3 weeks...
August 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27174392/an-injectable-hydrogel-as-bone-graft-material-with-added-antimicrobial-properties
#8
Giacomo Tommasi, Stefano Perni, Polina Prokopovich
Currently, the technique which provides the best chances for a successful bone graft, is the use of bone tissue from the same patient receiving it (autograft); the main limitations are the limited availability and the risks involved in removing living bone tissue, for example, explant site pain and morbidity. Allografts and xenografts may overcome these limitations; however, they increase the risk of rejection. For all these reasons the development of an artificial bone graft material is particularly important and hydrogels are a promising alternative for bone regeneration...
June 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27107475/maxillary-sinus-grafting-with-fresh-frozen-allograft-versus-bovine-bone-mineral-a-tomographic-and-histological-study
#9
Samuel Porfirio Xavier, Thiago de Santana Santos, Felipe Perraro Sehn, Erick Ricardo Silva, João de Andrade Garcez-Filho, Paulo Ricardo Saquete Martins-Filho
We evaluated histologically and tomographically the effects of fresh frozen bone allograft (FFB) or bovine bone mineral (BBM) in maxillary sinus floor augmentations. In total, 30 maxillary sinuses from 30 patients (mean age = 51.17 ± 10.86 years) underwent sinus augmentation. Patients were divided in two test groups (15 sinuses each). The first group was grafted with allograft bone, and the second group received bovine bone mineral. After 6 months, bone samples from each group were collected for histological examination...
June 2016: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/26873750/clinical-trial-and-in-vitro-study-comparing-the-efficacy-of-treating-bony-lesions-with-allografts-versus-synthetic-or-highly-processed-xenogeneic-bone-grafts
#10
Eva Johanna Kubosch, Anke Bernstein, Laura Wolf, Tobias Fretwurst, Katja Nelson, Hagen Schmal
BACKGROUND: Our study aim was to compare allogeneic cancellous bone (ACB) and synthetic or highly-processed xenogeneic bone substitutes (SBS) in the treatment of skeletal defects in orthopedic surgery. METHODS: 232 patients treated for bony lesions with ACB (n = 116) or SBS (n = 116) within a 10-year time period were included in this case-control study. Furthermore, both materials were seeded with human osteoblasts (hOB, n = 10) and analyzed by histology, for viability (AlamarBlue®) and protein expression activity (Luminex®)...
2016: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/26830666/lipus-promotes-spinal-fusion-coupling-proliferation-of-type-h-microvessels-in-bone
#11
Ximing Xu, Fei Wang, Yahong Yang, Xiaoyi Zhou, Yajun Cheng, Xianzhao Wei, Ming Li
Low-intensity pulsed ultrasound (LIPUS) has been found to accelerate spinal fusion. Type H microvessels are found in close relation with bone development. We analyzed the role of type H vessels in rat spinal fusion model intervened by LIPUS. It was found LIPUS could significantly accelerate bone fusion rate and enlarge bone callus. Osteoblasts were specifically located on the bone meshwork of the allograft, and were surrounded by type H microvessels. LIPUS could significantly increase the quantity of osteoblasts during spine fusion, which process was coupled with elevated angiogenesis of type H microvessels...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26814714/osteoinductive-potential-of-4-commonly-employed-bone-grafts
#12
Richard J Miron, Qiao Zhang, Anton Sculean, Daniel Buser, Benjamin E Pippenger, Michel Dard, Yoshinori Shirakata, Fatiha Chandad, Yufeng Zhang
OBJECTIVES: Guided bone regeneration (GBR) aims to predictably restore missing bone that has been lost due to trauma, periodontal disease or a variety of systemic conditions. Critical to this procedure is the ability of a bone grafting material to predictably serve as a 3-dimensional scaffold capable of inducing cell and bone tissue in-growth at the material surface. Although all bone grafts are osteoconductive to bone-forming osteoblasts, only a small number of commercially available bone grafts with FDA approval are osteoinductive including demineralized freeze-dried bone allographs (DFDBA) and scaffolds containing bone morphogenetic proteins (BMPs)...
January 27, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/26640621/polymeric-vs-hydroxyapatite-based-scaffolds-on-dental-pulp-stem-cell-proliferation-and-differentiation
#13
Arash Khojasteh, Saeed Reza Motamedian, Maryam Rezai Rad, Mehrnoosh Hasan Shahriari, Nasser Nadjmi
AIM: To evaluate adhesion, proliferation and differentiation of human dental pulp stem cells (hDPSCs) on four commercially available scaffold biomaterials. METHODS: hDPSCs were isolated from human dental pulp tissues of extracted wisdom teeth and established in stem cell growth medium. hDPSCs at passage 3-5 were seeded on four commercially available scaffold biomaterials, SureOss (Allograft), Cerabone (Xenograft), PLLA (Synthetic), and OSTEON II Collagen (Composite), for 7 and 14 d in osteogenic medium...
November 26, 2015: World Journal of Stem Cells
https://www.readbyqxmd.com/read/26227281/osteoinductive-potential-of-a-novel-biphasic-calcium-phosphate-bone-graft-in-comparison-with-autographs-xenografts-and-dfdba
#14
Richard J Miron, Anton Sculean, Yang Shuang, Dieter D Bosshardt, Reinhard Gruber, Daniel Buser, Fatiha Chandad, Yufeng Zhang
OBJECTIVES: Since the original description of osteoinduction in the early 20th century, the study and development of innovative biomaterials has emerged. Recently, novel synthetic bone grafts have been reported with potential to form ectopic bone in vivo. However, their full characterization in comparison with other leading bone grafts has not been investigated. The aim of this study was to determine the osteoinductive potential of bone grafts by comparing autogenous bone grafts, demineralized freeze-dried bone allografts (DFDBA), a commonly utilized natural bone mineral (NBM) from bovine origin (Bio-Oss), and a newly developed biphasic calcium phosphate (BCP)...
June 2016: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/26199628/bernese-periacetabular-osteotomy-in-a-hip-extra-articular-resection-followed-by-reconstruction-using-an-extracorporeal-irradiated-acetabulum-autograft-with-megaprosthesis-for-proximal-femur-osteosarcoma-in-a-pediatric-patient
#15
Victor Barro, Roberto Velez, Daniel Pacha, Jordi Giralt, Isabel Roca, Marius Aguirre
Osteosarcoma is the most common primary malignant tumour of bone. The oncologic surgery of a proximal femur osteosarcoma affecting the hip joint can be very challenging. We present an 8-year-old boy with a 5-month history of right hip pain. Radiographs and magnetic resonance imaging (MRI) showed a lytic lesion of the proximal femur extending 13 cm to the diaphysis. Histological evaluation was consistent with high-grade osteoblastic osteosarcoma. After completing chemotherapy we performed an extra-articular resection of the hip...
2015: Case Reports in Medicine
https://www.readbyqxmd.com/read/26084504/in-vitro-effect-of-mineralized-and-demineralized-bone-allografts-on-proliferation-and-differentiation-of-mg-63-osteoblast-like-cells
#16
Ardeshir Lafzi, Surena Vahabi, Shadab Ghods, Maryam Torshabi
Due to the extensive use of bone allografts in bone reconstruction and periodontal therapy as suitable alternatives to autografts, they are now marketed under different commercial brands. Considering the controversial reports regarding the osteoinductive properties of bone allografts, this study sought to assess the effect of type (mineralized/demineralized), amount and particle size of several allografts on the proliferation and differentiation of MG-63 osteoblast-like cells. MG-63 cells (24-h culture) were exposed to 20 and 40 mg amounts of nine different commercially available freeze-dried bone allografts...
March 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/25965275/significance-of-the-immunohistochemical-expression-of-bone-morphogenetic-protein-4-in-bone-maturation-after-maxillary-sinus-grafting-in-humans
#17
Laura Torrecillas-Martínez, Pablo Galindo-Moreno, Gustavo Ávila-Ortiz, Inmaculada Ortega-Oller, Alberto Monje, Pedro Hernández-Cortés, David Aguilar, Francisco O'Valle
BACKGROUND: Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β (TGFβ) protein superfamily and are known to be involved in bone and cartilage formation. Within this family, BMP-4 is one of the most studied members. It has been shown to induce osteogenic differentiation of osteoblasts and osteoprogenitor cells in vitro, but the intimate processes in which this protein promotes and regulates osseous repair still remains unclear. PURPOSE: To assess whether the native cellular immunohistochemical expression of BMP-4 correlates with the maturation of bone samples obtained at 6 months after maxillary sinus augmentation...
August 2016: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/25563684/-the-use-of-structural-proximal-tibial-allografts-coated-with-human-albumin-in-treating-extensive-periprosthetic-knee-joint-bone-deficiency-and-averting-late-complications-case-report
#18
Tamás Klára, Lajos Csönge, Gábor Janositz, Károly Pap, Zsombor Lacza
The authors report the history of a 74-year-old patient who underwent surgical treatment for segmental knee-joint periprosthetic bone loss using structural proximal tibial allografts coated with serum albumin. Successful treatment of late complications which occurred in the postoperative period is also described. The authors emphasize that bone replacement with allografts is a physiological process that enables the stable positioning of the implant and the reconstruction of the soft tissues, the replacement of extensive bone loss, and also it is a less expensive operation...
January 11, 2015: Orvosi Hetilap
https://www.readbyqxmd.com/read/25539497/geometry-sensing-through-por1-regulates-rac1-activity-controlling-early-osteoblast-differentiation-in-response-to-nanofiber-diameter
#19
A M Higgins, B L Banik, J L Brown
Bone grafting procedures in the United States rely heavily upon autografts and allografts, which are donor-dependent, cause donor site pain, and can transmit disease. Synthetic bone grafts can reduce these risks; however, synthetics lack the bone differentiating (osteoinductive) abilities of auto- and allografts. Achieving innate osteoinductive properties of synthetics through surface modifications is currently under investigation. This study focuses on nanofibers, with emphasis on how fiber diameter and the potential curvature sensor POR1 affect the activation of the signaling molecules Rac1 and Arf1, and leading to expression of alkaline phosphatase (ALP), an osteoinductive marker...
February 2015: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/25479410/mesenchymal-stem-cells-promote-liver-regeneration-and-prolong-survival-in-small-for-size-liver-grafts-involvement-of-c-jun-n-terminal-kinase-cyclin-d1-and-nf-%C3%AE%C2%BAb
#20
Weijie Wang, Zhiyong Du, Jiqi Yan, Di Ma, Minmin Shi, Mingjun Zhang, Chenghong Peng, Hongwei Li
BACKGROUND: The therapeutic potential of mesenchymal stem cells (MSCs) has been highlighted recently for treatment of acute or chronic liver injury, by possibly differentiating into hepatocyte-like cells, reducing inflammation, and enhancing tissue repair. Despite recent progress, exact mechanisms of action are not clearly elucidated. In this study, we attempted to explore whether and how MSCs protected hepatocytes and stimulated allograft regeneration in small-for-size liver transplantation (SFSLT)...
2014: PloS One
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