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https://www.readbyqxmd.com/read/28345127/zoledronate-promotes-bone-formation-by-blocking-osteocyte-osteoblast-communication-during-bone-defect-healing
#1
Pingping Cui, Hongrui Liu, Jing Sun, Norio Amizuka, Qinfeng Sun, Minqi Li
Nitrogen-containing bisphosphonates (N-BPs) are potent antiresorptive drugs and their actions on osteoclasts have been studied extensively. Recent studies have suggested that N-BPs also target bone-forming cells. However, the precise mechanism of N-BPs in osteoblasts is paradoxical, and the specific role of osteocytes is worthy of in-depth study. Here, we investigated the cellular mechanisms of N-BPs regulating bone defect healing by zoledronate (ZA). Bone histomorphometry confirmed an increase in new bone formation by systemic ZA administration...
March 27, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28344398/evaluation-of-tissue-engineered-bone-constructs-using-rabbit-fetal-osteoblasts-on-acellular-bovine-cancellous-bone-matrix
#2
Rashmi, Rekha Pathak, Amarpal, H P Aithal, P Kinjavdekar, A M Pawde, A K Tiwari, P Sangeetha, P Tamilmahan, A B Manzoor
AIM: The aim of this study was to generate composite bone graft and investigate the rabbit fetal osteoblasts adhesion, proliferation and penetration on acellular matrices of cancellous bone. MATERIALS AND METHODS: Acellular cancellous bone was prepared and developed as in the previous study with little modification. These matrices were decellularized by rapid freeze and thaw cycle. To remove the cell debris, they were then treated with hydrogen peroxide (3%) and ethanol to remove antigenic cellular and nuclear materials from the scaffold...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28332281/collagen-type-xv-and-the-osteogenic-status
#3
Gina Lisignoli, Elisabetta Lambertini, Cristina Manferdini, Elena Gabusi, Letizia Penolazzi, Francesca Paolella, Marco Angelozzi, Veronica Casagranda, Roberta Piva
We have previously demonstrated that collagen type XV (ColXV) is a novel bone extracellular matrix (ECM) protein. It is well known that the complex mixture of multiple components present in ECM can help both to maintain stemness or to promote differentiation of stromal cells following change in qualitative characteristics or concentrations. We investigated the possible correlation between ColXV expression and mineral matrix deposition by human mesenchymal stromal cells (hMSCs) with different osteogenic potential and by osteoblasts (hOBs) that are able to grow in culture medium with or without calcium...
March 22, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28332213/hen-egg-york-phosvitin-stimulates-osteoblast-differentiation-in-the-absence-of-ascorbic-acid
#4
Qingli Liu, Chunyan Li, Fang Geng, Xi Huang, Meihu Ma
BACKGROUND: Egg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on the osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3-E1 cells by using alkaline phosphatase (ALP) activity analysis, alizarin red S staining and real-time PCR assay. RESULTS: ALP activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3-E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28325836/fibronectins-containing-extradomain-a-or-b-enhance-osteoblast-differentiation-via-distinct-integrins
#5
Carla Sens, Katrin Huck, Stefan Pettera, Stephan Uebel, Guido Wabnitz, Markus Moser, Inaam A Nakchbandi
Fibronectin is a multidomain protein secreted by various cell types. It forms a network of fibers within the extracellular matrix, and impacts intracellular processes by binding to various molecules, primarily integrin receptors on the cells. Both the presence of several isoforms and the ability of the various domains and isoforms to bind to a variety of integrins result in a wide range of effects. In vivo findings suggest that fibronectin isoforms produced by the osteoblasts enhance their differentiation. Here we report that the isoform characterized by the presence of extra-domain A (EDA) activates α4β1 integrin and augments osteoblast differentiation...
March 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28324674/osteoblast-osteoclast-interactions
#6
Xiao Chen, Zhongqiu Wang, Na Duan, Guoying Zhu, Edward M Schwarz, Chao Xie
Bone homeostasis depends on the resorption of bones by osteoclasts and formation of bones by the osteoblasts. Imbalance of this tightly coupled process can cause diseases such as osteoporosis. Thus, the mechanisms that regulate communication between osteoclasts and osteoblasts are critical to bone cell biology. It has been shown that osteoblasts and osteoclasts can communicate with each other through direct cell-cell contact, cytokines, and extracellular matrix interaction. Osteoblasts can affect osteoclast formation, differentiation, or apoptosis through several pathways, such as OPG/RANKL/RANK, RANKL/LGR4/RANK, Ephrin2/ephB4, and Fas/FasL pathways...
February 8, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28323143/effect-of-age-and-gender-on-serum-periostin-relationship-to-cortical-measures-bone-turnover-and-hormones
#7
Jennifer S Walsh, Fatma Gossiel, Jessica R Scott, Margaret A Paggiosi, Richard Eastell
Periostin is an extracellular matrix protein, and in bone is expressed most highly in the periosteum. It increases bone formation through osteoblast differentiation, cell adhesion, Wnt signalling and collagen cross-linking. We hypothesised that serum periostin would be high at times of life when cortical modeling is active, in early adulthood and in older age, and that it would correlate with cortical bone measures, bone turnover and hormones that regulate cortical modeling. We conducted a cross-sectional observational study of 166 healthy men and women at three skeletal stages; the end of longitudinal growth (16-18years), peak bone mass (30-32years) and older age (over 70years)...
March 16, 2017: Bone
https://www.readbyqxmd.com/read/28321651/effects-of-strontium-ranelate-treatment-on-osteoblasts-cultivated-onto-scaffolds-of-trabeculae-bovine-bone
#8
Gerluza Aparecida Borges Silva, Bruno Machado Bertassoli, Cristiane Aparecida Sousa, Juliano Douglas Albergaria, Rayan Silva de Paula, Erika Cristina Jorge
Blocks of Bovine bone have shown promising results as implantable scaffolds to promote bone regeneration. Strontium ranelate (SrR) is both an antiresorptive and an anabolic drug that has been indicated for oral administration to treat osteoporosis. Few studies, however, have investigated the local effects of SrR and its use in association with biomaterials thus far. In this work, we investigated SrR effects in cultures of primary osteoblasts (PO, from Wistar rats calvaria) and immortalized osteoblasts (IO, from MC3T3-E1 cell line) cultivated as a monolayer or in association with scaffolds of bovine bone in mineralized (MBB) and demineralized (DBB) forms...
March 20, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/28318155/-biomineralization-of-electrospun-polycaprolactone-guided-bone-regeneration-membrane
#9
Deng Xia, Bai Shi
OBJECTIVE: To evaluate the biomineralization of the tissue-engineering electrospun polycaprolactone (PCL) scaffold and its potential use for guided bone regeneration (GBR) membranes. METHODS: PCL ultrafinefiber scaffolds were fabricated by electrospinning and then immersed in supersaturated calcification solution (SCS) for biomineralization investigation. The electrospun PCL scaffolds and the calcium phosphate coating were identified by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR)...
December 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28315346/inhibition-of-osteoporosis-by-the-%C3%AE-v%C3%AE-3-integrin-antagonist-of-rhodostomin-variants
#10
Tzu-Hung Lin, Rong-Sen Yang, Huang-Ju Tu, Houng-Chi Liou, Yen-Ming Lin, Woie-Jer Chuang, Wen-Mei Fu
Integrins are heterodimeric cell surface receptors that mediate cell-cell and cell-matrix interaction. The vitronectin and osteopontin receptor αvβ3 integrin has increased expression levels and is implicated in the adhesion, activation, and migration of osteoclasts on the bone surface as well as osteoclast polarization. αvβ3 integrin plays an important role in osteoclast differentiation and resorption. In addition, Arg-Gly-Asp (RGD)-containing peptides, small molecular inhibitors, and antibodies to αvβ3 integrin have been shown to inhibit bone resorption in vitro and in vivo...
March 14, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28302495/neural-egfl-like-1-is-a-downstream-regulator-of-runt-related-transcription-factor-2-in-chondrogenic-differentiation-and-maturation
#11
Chenshuang Li, Jie Jiang, Zhong Zheng, Kevin S Lee, Yanheng Zhou, Eric Chen, Cymbeline T Culiat, Yiqiang Qiao, Xuepeng Chen, Kang Ting, Xinli Zhang, Chia Soo
Recent studies indicate that neural EGFL-like 1 (Nell-1), a secretive extracellular matrix molecule, is involved in chondrogenic differentiation. Herein, we demonstrated that Nell-1 serves as a key downstream target of runt-related transcription factor 2 (Runx2), a central regulator of chondrogenesis. Unlike in osteoblast lineage cells where Nell-1 and Runx2 demonstrate mutual regulation, further studies in chondrocytes revealed that Runx2 tightly regulates the expression of Nell-1; however, Nell-1 does not alter the expression of Runx2...
March 13, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28299448/capacity-of-muscle-derived-stem-cells-and-pericytes-to-promote-tendon-graft-integration-and-ligamentization-following-anterior-cruciate-ligament-reconstruction
#12
Tomislav Ćuti, Maja Antunović, Inga Marijanović, Alan Ivković, Andreja Vukasović, Igor Matić, Marko Pećina, Damir Hudetz
PURPOSE: The aim of this study is to examine the capacity of muscle tissue preserved on hamstring tendons forming candy-stripe grafts in order to improve tendon to bone ingrowth and ligamentization. We hypothesized that muscle tissue does possess a stem cell population that could enhance the healing process of the ACL graft when preserved on the tendons. METHODS: Human samples from gracilis and semitendinosus muscles were collected during ACL surgery from ten patients and from these tissue samples human muscle-derived stem cells and tendon-derived stem cells were isolated and propagated...
March 15, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28296389/abrogated-cell-contact-guidance-on-amino-functionalized-microgrooves
#13
Caroline Mörke, Henrike Rebl, Birgit Finke, Manuela Dubs, Peter Nestler, Aissam Airoudj, Vincent Roucoules, Matthias Schnabelrauch, Andreas Körtge, Karine Anselme, Christiane A Helm, J Barbara Nebe
Topographical and chemical features of biomaterial surfaces affect the cell physiology at the interface and are promising tools for the improvement of implants. The dominance of the surface topography on cell behavior is often accentuated. Striated surfaces induce an alignment of cells and their intracellular adhesion-mediated components. Recently, it could be demonstrated that a chemical modification via plasma polymerized allylamine was not only able to boost osteoblast cell adhesion and spreading but also override the cell alignment on stochastically machined titanium...
March 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28272740/comparative-proteomic-profiling-of-human-osteoblast-derived-extracellular-matrices-identifies-proteins-involved-in-mesenchymal-stromal-cell-osteogenic-differentiation-and-mineralization
#14
Marta Baroncelli, Bram C van der Eerden, Yik-Yang Kan, Rodrigo D Alves, Jeroen A Demmers, Jeroen van de Peppel, Johannes P van Leeuwen
The extracellular matrix (ECM) is a dynamic component of tissue architecture that physically supports cells and actively influences their behavior. In the context of bone regeneration, cell-secreted ECMs have become of interest as they reproduce tissue-architecture and modulate the promising properties of mesenchymal stem cells (MSCs). We have previously created an in vitro model of human osteoblast-derived devitalized ECM that was osteopromotive for MSCs. The aim of this study was to identify ECM regulatory proteins able to modulate MSC differentiation to broaden the spectrum of MSC clinical applications...
March 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28271209/tgf-%C3%AE-1-impairs-mechanosensation-of-human-osteoblasts-via-hdac6-mediated-shortening-and-distortion-of-primary-cilia
#15
Sabrina Ehnert, Vrinda Sreekumar, Romina H Aspera-Werz, Sahar O Sajadian, Elke Wintermeyer, Gunther H Sandmann, Christian Bahrs, Jan G Hengstler, Patricio Godoy, Andreas K Nussler
Transforming growth factor β (TGF-β) is a critical regulator of bone density owing to its multiple effects on cell growth and differentiation. Recently, we have shown that TGF-β1 effectively blocks bone morphogenetic protein (BMP) induced maturation of osteoblasts by upregulating histone deacetylase (HDAC) activity. The current study aimed at investigating the effect of rhTGF-β1 treatment on the expression of specific HDACs and their cellular effects, e.g., microtubule structures (primary cilia) and mechanosensation...
March 7, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28266351/biofabrication-of-a-co-culture-system-in-an-osteoid-like-hydrogel-matrix
#16
Tobias Zehnder, Aldo Boccaccini, Rainer Detsch
Biofabrication aims to develop functional, biological constructs using automated processes (additive manufacturing, AM) involving different cell types and biomaterials [1]. As bone tissue is based on the crosstalk between osteoblasts and osteoclasts at least, evaluating cell-cell and cell-material interactions is of interest to understand bone remodeling. There is increasing interest in the role of osteoclasts not only considering bone resorption, but also their influence on the proliferation, migration and differentiation of osteoblasts...
March 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28265268/brucella-and-osteoarticular-cell-activation-partners-in-crime
#17
REVIEW
Guillermo H Giambartolomei, Paula C Arriola Benitez, M Victoria Delpino
Osteoarticular brucellosis is the most common presentation of human active disease although its prevalence varies widely. The three most common forms of osteoarticular involvement are sacroiliitis, spondylitis, and peripheral arthritis. The molecular mechanisms implicated in bone damage have been recently elucidated. B. abortus induces bone damage through diverse mechanisms in which TNF-α and the receptor activator of nuclear factor kappa-B ligand (RANKL)-the natural modulator of bone homeostasis are involved...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28264787/low-level-laser-therapy-modulates-viability-alkaline-phosphatase-and-matrix-metalloproteinase-2-activities-of-osteoblasts
#18
Flávia Amadeu de Oliveira, Adriana Arruda Matos, Sandra Satiko Matsuda, Marília Afonso Rabelo Buzalaf, Vanderley Salvador Bagnato, Maria Aparecida de Andrade Moreira Machado, Carla Andreotti Damante, Rodrigo Cardoso de Oliveira, Camila Peres-Buzalaf
Low level laser therapy (LLLT) has been shown to stimulate bone cell metabolism but their impact on the matrix metalloproteinase (MMP) expression and activity is little explored. This study evaluated the influence of LLLT at two different wavelengths, red and infrared, on MC3T3-E1 preosteoblast viability, alkaline phosphatase (ALP) and MMP-2 and -9 activities. To accomplish this, MC3T3-E1 cells were irradiated with a punctual application of either red (660nm; InGaAIP active medium) or infrared (780nm; GaAlAs active medium) lasers both at a potency of 20mW, energy dose of 0...
February 28, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28264701/zoledronic-acid-alters-hematopoiesis-and-generates-breast-tumor-suppressive-bone-marrow-cells
#19
Jessalyn M Ubellacker, Marie-Therese Haider, Molly J DeCristo, Gloria Allocca, Nicola J Brown, Daniel P Silver, Ingunn Holen, Sandra S McAllister
BACKGROUND: The bone-targeting agent zoledronic acid (ZOL) increases breast cancer survival in subsets of patients, but the underlying reasons for this protective effect are unknown. ZOL modulates the activity of osteoclasts and osteoblasts, which form hematopoietic stem cell niches, and therefore may affect hematopoietic cells that play a role in breast cancer progression. METHOD: Immunocompetent and immunocompromised strains of mice commonly used for breast cancer research were injected with a single, clinically relevant dose of ZOL (100 μg/kg) or vehicle control...
March 6, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28263431/electrospun-oriented-gelatin-hydroxyapatite-fibre-scaffolds-for-bone-tissue-engineering
#20
Ali A Salifu, Constantina Lekakou, Fatima Labeed
Tissue engineering of human foetal osteoblast (hFOB) cells was investigated on gelatin-hydroxyapatite (HA), crosslinked, electrospun oriented fibre scaffolds at the different hydroxyapatite concentrations of 0, 10, 20 and 25 wt% in the dry fibres and different fibre diameter, pore size and porosity of scaffolds. Rheological tests and proton NMR spectroscopy were conducted for all solutions used for electrospinning. It was found that 25 wt% HA-gelatin scaffolds electrospun at 20 kV led to the greatest cell attachment, cell proliferation and extracellular matrix (ECM) production while fibre orientation improved the mechanical properties, where crosslinked electrospun 25 wt% HA-gelatin fibre scaffolds yielded a Young's modulus in the range of 0...
March 6, 2017: Journal of Biomedical Materials Research. Part A
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