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https://www.readbyqxmd.com/read/28930090/biomimetic-matrix-fabricated-by-lmp-1-gene-transduced-mc3t3-e1-cells-for-bone-regeneration
#1
Junxuan Ma, Wei Guo, Manman Gao, Baoding Huang, Qihua Qi, Zemin Ling, Yan Chen, Hao Hu, Hailian Zhou, Fei Yu, Ke Chen, Zhiyu Zhou, Deming Xiao, Xuenong Zou
Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). The study aimed to mimic native osteoinductive microenvironment by developing an ECM using gene-transduced cells. LIM mineralization protein-1 (LMP-1) gene was transferred to the murine pre-osteoblast cells (MC3T3-E1) using lentiviral vectors. Western blotting essay indicated that the MC3T3-E1 cells expressed an increased level of bone morphologic protein-2, -4 and-7 (BMP-2, -4 and -7) after LMP-1 gene transduction...
September 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28928248/carbonic-anhydrase-iii-protects-osteocytes-from-oxidative-stress
#2
Chao Shi, Yuhei Uda, Christopher Dedic, Ehab Azab, Ningyuan Sun, Amira I Hussein, Christopher A Petty, Keertik Fulzele, Maria C Mitterberger-Vogt, Werner Zwerschke, Renata Pereira, Kunzheng Wang, Paola Divieti Pajevic
Osteocytes are master orchestrators of bone remodeling; they control osteoblast and osteoclast activities both directly via cell-to-cell communication and indirectly via secreted factors, and they are the main postnatal source of sclerostin and RANKL (receptor activator of NF-kB ligand), two regulators of osteoblast and osteoclast function. Despite progress in understanding osteocyte biology and function, much remains to be elucidated. Recently developed osteocytic cell lines-together with new genome editing tools-has allowed a closer look at the biology and molecular makeup of these cells...
September 19, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28927931/polyurethane-foam-scaffold-as-in-vitro-model-for-breast-cancer-bone-metastasis
#3
Valentina Angeloni, Nicola Contessi, Cinzia De Marco, Serena Bertoldi, Maria Cristina Tanzi, Maria Grazia Daidone, Silvia Farè
Breast cancer (BC) represents the most incident cancer case in women (29%), with high mortality rate. Bone metastasis occurs in 20-50% cases and, despite advances in BC research, the interactions between tumor cells and the metastatic microenvironment are still poorly understood. In vitro 3D models gained great interest in cancer research, thanks to the reproducibility, the 3D spatial cues and associated low costs, compared to in vivo and 2D in vitro models. In this study, we investigated the suitability of a poly-ether-urethane (PU) foam as 3D in vitro model to study the interactions between BC tumor-initiating cells and the bone microenvironment...
September 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28926103/fam20c-regulates-osteoblast-behaviors-and-intracellular-signaling-pathways-in-a-cell-autonomous-manner
#4
Chao Liu, Hua Zhang, Priyam Jani, Xiaofang Wang, Yongbo Lu, Nan Li, Jing Xiao, Chunlin Qin
Recent studies indicate that Family with sequence similarity 20 member C (FAM20C) catalyzes the phosphorylation of secreted proteins, and participates in a variety of biological processes, including cell proliferation, migration, mineralization and phosphate homeostasis. To explore the local influences of FAM20C on osteoblast, Fam20c-deficient osteoblasts were generated by treating the immortalized Fam20c(f/f) osteoblasts with CMV-Cre-IRES-EGFP lentivirus. Compared with the normal Fam20c(f/f) osteoblasts, the expression of Bone sialoprotein (Bsp), Osteocalcin (Ocn), Fibroblast growth factor 23 (Fgf23) and transcription factors that promote osteoblast maturation were up-regulated in the Fam20c-deficient osteoblasts...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28921936/sprouty2-is-involved-in-the-control-of-osteoblast-proliferation-and-differentiation-through-the-fgf-and-bmp-signaling-pathways
#5
Takaharu Taketomi, Tomohiro Onimura, Daigo Yoshiga, Daichi Muratsu, Terukazu Sanui, Takao Fukuda, Jingo Kusukawa, Seiji Nakamura
Fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) play essential roles in bone formation and osteoblast activity through the extracellular signal regulated kinase 1/2 (ERK1/2) and Smad pathways. Sprouty family members are intracellular inhibitors of the FGF signaling pathway, and four orthologs of Sprouty have been identified in mammals. In vivo analyses have revealed that Sprouty2 is associated with bone formation. However, the mechanism by which the Sprouty family controls bone formation has not been clarified...
September 16, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28921822/construction-of-human-induced-pluripotent-stem-cell-derived-oriented-bone-matrix-microstructure-by-using-in-vitro-engineered-anisotropic-culture-model
#6
Ryosuke Ozasa, Aira Matsugaki, Yoshihiro Isobe, Taro Saku, Hui-Suk Yun, Takayoshi Nakano
Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold...
September 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28919027/macrolactin-f-inhibits-rankl-mediated-osteoclastogenesis-by-suppressing-akt-mapk-and-nfatc1-pathways-and-promotes-osteoblastogenesis-through-a-bmp-2-smad-akt-runx2-signaling-pathway
#7
Liang Li, Mahesh Sapkota, Ming Gao, Hyukjae Choi, Yunjo Soh
The balance between bone formation and bone resorption is maintained by osteoblasts and osteoclasts. In the current study, macrolactin F (MF) was investigated for novel biological activity on the receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclastogenesis in primary bone marrow-derived macrophages (BMMs). We found that RANKL-induced osteoclast formation and differentiation from BMMs was significantly inhibited by MF in a dose-dependent manner without cytotoxicity. RANKL-induced F-actin ring formation and bone resorption activity in BMMs which was attenuated by MF...
September 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28914969/autocrine-and-paracrine-regulation-of-the-murine-skeleton-by-osteocyte-derived-parathyroid-hormone-related-protein
#8
Niloufar Ansari, Patricia W M Ho, Blessing Crimeen-Irwin, Ingrid J Poulton, Athena R Brunt, Mark R Forwood, Paola Divieti Pajevic, Jonathan H Gooi, T John Martin, Natalie A Sims
Parathyroid hormone related-protein (PTHrP) and parathyroid hormone (PTH) have N-terminal domains that bind a common receptor, PTHR1. N-terminal PTH (teriparatide) and now a modified N-terminal PTHrP (abaloparatide) are FDA-approved therapies for osteoporosis. In physiology, 00PTHrP does not normally circulate at significant levels, but acts locally and osteocytes, cells residing within the bone matrix, express both PTHrP and the PTHR1. Since PTHR1 in osteocytes is required for normal bone resorption, we determined how osteocyte-derived PTHrP influences the skeleton...
September 15, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28910401/strontium-doped-hydroxyapatite-polysaccharide-materials-effect-on-ectopic-bone-formation
#9
C Ehret, R Aid-Launais, T Sagardoy, R Siadous, R Bareille, S Rey, S Pechev, L Etienne, J Kalisky, E de Mones, D Letourneur, J Amedee Vilamitjana
Previous studies performed using polysaccharide-based matrices supplemented with hydroxyapatite (HA) particles showed their ability to form in subcutaneous and intramuscular sites a mineralized and osteoid tissue. Our objectives are to optimize the HA content in the matrix and to test the combination of HA with strontium (Sr-HA) to increase the matrix bioactivity. First, non-doped Sr-HA powders were combined to the matrix at three different ratios and were implanted subcutaneously for 2 and 4 weeks. Interestingly, matrices showed radiolucent properties before implantation...
2017: PloS One
https://www.readbyqxmd.com/read/28910136/nrp1-accelerates-odontoblast-differentiation-of-dental-pulp-stem-cells-through-classical-wnt-%C3%AE-catenin-signaling
#10
Yihua Song, Xiaojuan Liu, Xingmei Feng, Zhifeng Gu, Yongchun Gu, Min Lian, Jingwen Xiao, Peipei Cao, Ke Zheng, Xiaobing Gu, Dongping Li, Ping He, Chenfei Wang
Neuropilin-1 (NRP1) is one of the members of neuropilin family. It can combine with disparate ligands involved in regulating cell proliferation, apoptosis, and differentiation. The binding of NRP1 to Sema3A stimulates osteoblast differentiation through the classical Wnt/β-catenin pathway. However, the functions of NRP1 in dental pulp stem cells (DPSCs) are not clear. The aim of our study was to investigate how NRP1 controlled odontoblast differentiation in DPSCs and clarified the underlying mechanisms. NRP1 expression was increased in time-dependent manner along with cell odontoblast differentiation...
September 14, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28898756/bioreactor-culture-duration-of-engineered-constructs-influences-bone-formation-by-mesenchymal-stem-cells
#11
Debika Mitra, Jacklyn Whitehead, Osamu W Yasui, J Kent Leach
Perfusion culture of mesenchymal stem cells (MSCs) seeded in biomaterial scaffolds provides nutrients for cell survival, enhances extracellular matrix deposition, and increases osteogenic cell differentiation. However, there is no consensus on the appropriate perfusion duration of cellular constructs in vitro to boost their bone forming capacity in vivo. We investigated this phenomenon by culturing human MSCs in macroporous composite scaffolds in a direct perfusion bioreactor and compared their response to scaffolds in continuous dynamic culture conditions on an XYZ shaker...
September 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28891249/poly-vinylphosphonic-acid-co-acrylic-acid-hydrogels-the-effect-of-copolymer-composition-on-osteoblast-adhesion-and-proliferation
#12
Rebecca E Dey, Ian Wimpenny, Julie E Gough, David C Watts, Peter M Budd
There is a clinical need for a synthetic bone graft substitute that can be used at sites of surgical intervention to promote bone regeneration. Poly(vinylphosphonic acid-co-acrylic acid) (PVPA-co-AA) has recently been identified as a potential candidate for use in bone tissue scaffolds. It is hypothesised that PVPA-co-AA can bind to divalent calcium ions on bone mineral surfaces to control matrix mineralisation and promote bone formation. In this study, hydrogels of PVPA-co-AA have been produced and the effect of copolymer composition on the structure and properties of the gels was investigated...
September 11, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28887955/engineered-electrospun-polycaprolactone-pcl-octacalcium-phosphate-ocp-scaffold-for-bone-tissue-engineering
#13
Zohre Heydari, Davod Mohebbi-Kalhori, Mahdi Shafiee Afarani
The main challenge in bone tissue engineering is to find suitable biological substitutes which act as scaffolds. Hence, in this work, a novel scaffold composed of octacalcium phosphate (OCP) particles fabricated by co-precipitation method and polycaprolactone (PCL) using electrospinning technique was introduced. The electrospun scaffolds were characterized by SEM, FTIR, XRD, DSC and TGA analysis. The mechanical properties of the composite scaffolds including maximum tensile stress, strain at break and Young modulus were measured...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887147/both-ligand-and-vdr-expression-levels-critically-determine-the-effect-of-1%C3%AE-25-dihydroxyvitamin-d3-on-osteoblast-differentiation
#14
Dongqing Yang, Paul H Anderson, Asiri R Wijenayaka, Kate R Barratt, Rahma Triliana, Catherine J M Stapledon, Hong Zhou, David M Findlay, Howard A Morris, Gerald J Atkins
Previous studies have shown that 1α,25-dihydroxyvitamin D3 (1,25D) through vitamin D receptor (VDR) signalling has both catabolic and anabolic effects on osteoblast differentiation. However, the mechanism of these differential effects by 1,25D is not fully understood. In this study, mice with three different genetic backgrounds, representing a normal VDR level (wild-type, WT), VDR over-expression specifically in mature osteoblasts (ObVDR-B6) and global VDR knockout (VDRKO), were utilised to generate primary osteoblast-like cultures to further elucidate the effects of 1,25D on osteoblast differentiation...
September 5, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28883483/serpinb2-is-a-novel-tgf%C3%AE-responsive-lineage-fate-determinant-of-human-bone-marrow-stromal-cells
#15
Mona Elsafadi, Muthurangan Manikandan, Muhammad Atteya, Raed Abu Dawud, Sami Almalki, Zahid Ali Kaimkhani, Abdullah Aldahmash, Nehad M Alajez, Musaad Alfayez, Moustapha Kassem, Amer Mahmood
TGF-β1, a multifunctional regulator of cell growth and differentiation, is the most abundant bone matrix growth factor. During differentiation of human bone stromal cells (hBMSCs), which constitute bone marrow osteoblast (OS) and adipocyte (AD) progenitor cells, continuous TGF-β1 (10 ng/ml) treatment enhanced OS differentiation as evidenced by increased mineralised matrix production. Conversely, pulsed TGF-β1 administration during the commitment phase increased mature lipid-filled adipocyte numbers. Global gene expression analysis using DNA microarrays in hBMSCs treated with TGF-β1 identified 1587 up- and 1716 down-regulated genes in OS-induced, TGF-β1-treated compared to OS-induced hBMSCs (2...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28879686/development-of-a-bioactive-porous-collagen-%C3%AE-tricalcium-phosphate-bone-graft-assisting-rapid-vascularization-for-bone-tissue-engineering-applications
#16
Nafiseh Baheiraei, Mohamma Reza Nourani, Sm Javad Mortazavi, Mansoureh Movahedin, Hossein Eyni, Fatemeh Baghery, Mohammad Hadi Norahan
We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/β-TCP) scaffolds with a β-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrow-derived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was,also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28879314/protective-effects-of-remifentanil-against-h2o2-induced-oxidative-stress-in-human-osteoblasts
#17
Ji-Young Yoon, Do-Wan Kim, Eun-Jung Kim, Bong-Soo Park, Ji-Uk Yoon, Hyung-Joon Kim, Jeong-Hoon Park
BACKGROUND: Bone injury is common in many clinical situations, such as surgery or trauma. During surgery, excessive reactive oxygen species (ROS) production decreases the quality and quantity of osteoblasts. Remifentanil decreases ROS production, reducing oxidative stress and the inflammatory response. We investigated remifentanil's protective effects against H2O2-induced oxidative stress in osteoblasts. METHODS: To investigate the effect of remifentanil on human fetal osteoblast (hFOB) cells, the cells were incubated with 1 ng/ml of remifentanil for 2 h before exposure to H2O2...
December 2016: J Dent Anesth Pain Med
https://www.readbyqxmd.com/read/28875562/a-new-composite-hydrogel-combining-the-biological-properties-of-collagen-with-the-mechanical-properties-of-a-supramolecular-scaffold-for-bone-tissue-engineering
#18
Mathieu Maisani, Sophia Ziane, Camille Ehret, Lucie Levesque, Robin Siadous, Jean-François Le Meins, Pascale Chevalier, Philippe Barthélémy, Hugo De Oliveira, Joëlle Amédée, Diego Mantovani, Olivier Chassande
Tissue engineering is a promising alternative to autografts, allografts or biomaterials to address the treatment of severe and large bone lesions. Classically, tissue engineering products associate a scaffold and cells, and are implanted or injected into the lesion. These cells must be embedded in an appropriate biocompatible scaffold, which offers a favourable environment for their survival and differentiation. Here, we designed a composite hydrogel composed of collagen I, an extracellular matrix protein widely used in several therapeutic applications, which we associated with a physical hydrogel generated from a synthetic small amphiphilic molecule...
September 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28872686/weak-bones-in-diabetes-mellitus-an-update-on-pharmaceutical-treatment-options
#19
REVIEW
Daphne P L Lin, Crispin R Dass
OBJECTIVES: Diabetes mellitus is often associated with a number of complications such as nephropathy, neuropathy, retinopathy and foot ulcers. However, weak bone is a diabetic complication that is often overlooked. Although the exact mechanism for weak bones within diabetes mellitus is unclear, studies have shown that the mechanism does differ in both type I (T1DM) and type II diabetes (T2DM). This review, however, investigates the application of mesenchymal stem cells, recombinant human bone morphogenetic protein-2, teriparatide, insulin administration and the effectiveness of a peroxisome proliferator-activated receptor-ϒ modulator, netoglitazone in the context of diabetic weak bones...
September 5, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28871656/fabrication-of-a-biomimetic-zeinpda-nanofibrous-scaffold-impregnated-with-bmp-2-peptide-conjugated-tio2-nanoparticle-for-bone-tissue-engineering
#20
Babitha Sekar, Meenakshi Annamalai, Michal Marcin Dykas, Surajit Saha, Kingshuk Poddar, Jayarama Reddy Venugopal, Seeram Ramakrishna, Thirumalai Venkatesan, Purna Sai Korrapati
A biomimetic Zein polydopamine (PDA) based nanofiber scaffold was fabricated to deliver bone morphogenic protein-2 (BMP-2) peptide conjugated titanium dioxide (TiO2 ) nanoparticles in a sustained manner for investigating its osteogenic differentiation potential. To prolong its retention time at the target site, BMP-2 peptide has been conjugated to TiO2 nanoparticles owing to its high surface to volume ratio. The effect of biochemical cues from BMP-2 peptide and nano topographical stimulation of electrospun Zein PDA nanofibers were examined for its enhanced osteogenic expression of human fetal osteoblast (hFOB) cells...
September 4, 2017: Journal of Tissue Engineering and Regenerative Medicine
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