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https://www.readbyqxmd.com/read/29775615/extracellular-matrix-protein-dmp1-suppresses-osteogenic-differentiation-of-mesenchymal-stem-cells
#1
Shufan Zhang, Huixuan Wan, Peng Wang, Mengmeng Liu, Gongchen Li, Chunxue Zhang, Yao Sun
Mesenchymal Stem Cells (MSCs) are self-renewing and multipotent stem cells which was investigated for diverse clinical applications. However, complex mechanism of MSCs fate determination is still not fully disclosed. Extracellular matrix (ECM) proteins contribute to maintain MSCs stemness by providing extracellular microenvironment. Increasing evidences show that ECM proteins could also regulate the fate of MSCs directly. Dentin matrix protein 1 (DMP1) is an ECM protein enrich in bone tissue and terminal cells, which well-known in promoting osteoblasts and osteocytes maturation, and facilitate mineralization...
May 15, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29752107/macroporous-microbeads-containing-apatite-modified-mesoporous-bioactive-glass-nanofibres-for-bone-tissue-engineering-applications
#2
Fu-Yin Hsu, Hsien-Wen Hsu, Yu-Han Chang, Jing-Lun Yu, Lih-Rou Rau, Shiao-Wen Tsai
Mesoporous bioactive glass (MBG) has a greater surface area and pore volume than conventional BG. Hence, MBG is useful as a drug delivery carrier. Previously, MBG has been fabricated as dense or porous blocks. Compared to blocks, microbeads have a greater flexibility to fill different-shaped cavities with close packing. Moreover, fibrous materials have proven to increase cell attachment and differentiation because they mimic the three-dimensional structure of the natural extracellular matrix (ECM). Macroporous materials possess porous structures with interconnecting channels that allow the invasive growth of cells and capillaries...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29749440/vitamin-k%C3%A2-dependent-proteins-involved-in-bone-and-cardiovascular-health-review
#3
Lianpu Wen, Jiepeng Chen, Lili Duan, Shuzhuang Li
In postmenopausal women and elderly men, bone density decreases with age and vascular calcification is aggravated. This condition is closely associated with vitamin K2 deficiency. A total of 17 different vitamin K‑dependent proteins have been identified to date. Vitamin K‑dependent proteins are located within the bone, heart and blood vessels. For instance, carboxylated osteocalcin is beneficial for bone and aids the deposition of calcium into the bone matrix. Carboxylated matrix Gla protein effectively protects blood vessels and may prevent calcification within the vascular wall...
April 27, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29748441/maternal-embryonic-leucine-zipper-kinase-inhibitor-otssp167-has-preclinical-activity-on-multiple-myeloma-bone-disease
#4
Joséphine Muller, Arnold Bolomsky, Sophie Dubois, Elodie Duray, Kathrin Stangelberger, Erwan Plougonven, Margaux Lejeune, Angélique Léonard, Caroline Marty, Ute Hempel, Frédéric Baron, Yves Beguin, Martine Cohen-Solal, Heinz Ludwig, Roy Heusschen, Jo Caers
Multiple myeloma bone disease is characterized by an uncoupling of bone remodeling in the multiple myeloma microenvironment, resulting in the development of lytic bone lesions. Most myeloma patients suffer from these bone lesions, which not only causes morbidity but also negatively impacts survival. The development of novel therapies, ideally with a combined anti-resorptive and bone-anabolic effect, is of great interest because lesions persist with the current standard of care, even in patients in complete remission...
May 10, 2018: Haematologica
https://www.readbyqxmd.com/read/29745944/pegylated-graphene-oxide-mediated-quercetin-modified-collagen-hybrid-scaffold-for-enhancement-of-mscs-differentiation-potential-and-diabetic-wound-healing
#5
Jing Chu, Panpan Shi, Wenxia Yan, Jinping Fu, Zhi Yang, Chengmin He, Xiaoyuan Deng, Hanping Liu
Nanoscale delivery based on polyethylene glycol (PEG)ylated graphene oxide (GO-PEG) merits attention for biomedical applications owing to its functional surface modification, superior solubility/biocompatibility and controllable drug release capability. However, impaired skin regeneration in applications of these fascinating nanomaterials in diabetes is still limited, and critical issues need to be addressed regarding insufficient collagen hyperplasia and inadequate blood supply. Therefore, a high-performance tissue engineering scaffold with biocompatible and biodegradable properties is essential for diabetic wound healing...
May 10, 2018: Nanoscale
https://www.readbyqxmd.com/read/29745808/the-er-ca-2-sensor-stim1-can-activate-osteoblast-and-odontoblast-differentiation-in-mineralized-tissues
#6
Yinghua Chen, Amsaveni Ramachandran, Youbin Zhang, Rahul Koshy, Anne George
Bone and dentin development requires temporal and spatial deposition of calcium phosphate mineral. A host of proteins works in concert to contribute to this tightly regulated process while malfunction in this scheme often leads to pathological defects. We have reported earlier that DMP1 stimulation of preosteoblasts leads to calcium release from internal Ca2+ stores and this store depletion is sensed by the ER Ca2+ sensor STIM1 (stromal interaction molecule 1). In this study, we first assessed the temporal and spatial localization of STIM1 protein during the development of bone and dentin by immunohistochemical methods...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29744297/fgf-2-suppresses-expression-of-nephronectin-via-jnk-and-pi3k-pathways
#7
Tadashi Kato, Atsushi Yamada, Mikiko Ikehata, Yuko Yoshida, Kiyohito Sasa, Naoko Morimura, Akiko Sakashita, Takehiko Iijima, Daichi Chikazu, Hiroaki Ogata, Ryutaro Kamijo
Nephronectin (Npnt), an extracellular matrix protein, is a ligand for integrin α8β1 and is involved in the development of various organs, such as the kidneys, bones, liver, and muscles. Previously, we found that Npnt expression was inhibited by various cytokines including transforming growth factor-β (Tgf-β) and oncostatin M (Osm). Fibroblast growth factor (Fgf)-2, otherwise known as basic Fgf, also plays important roles in skeletal development and postnatal osteogenesis. In this study, Npnt expression was found to be suppressed by Fgf-2 in MC3T3-E1 cells, an osteoblast-like cell line, in a dose- and time-dependent manners...
May 2018: FEBS Open Bio
https://www.readbyqxmd.com/read/29741358/fibrous-topography-potentiated-canonical-wnt-signaling-directs-the-odontoblastic-differentiation-of-dental-pulp-derived-stem-cells
#8
Saeed Ur Rahman, Joung-Hwan Oh, Young-Dan Cho, Shin Hye Chung, Gene Lee, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms which underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29731830/rho-rho-associated-protein-kinase-signaling-pathway-mediated-downregulation-of-runt-related-transcription-factor-2-expression-promotes-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts
#9
Xiaoqing Huang, Xiaoling Chen, Hongbai Chen, Dongwei Xu, Chen Lin, Bin Peng
The present study investigated the role of runt-related transcription factor 2 (Runx2) in regulating the differentiation of human dental pulp stem cells (hDPSCs) into odontoblasts under the mediation of the Rho/Rho-associated protein kinase (ROCK) signaling pathway. hDPSCs and human bone marrow mesenchymal stem cells (hBMSCs) were mineralized to induce differentiation. The expression levels of odontoblast- and osteoblast-specific proteins, dentin sialophosphoprotein (DSPP), osteocalcin (OCN) and Runx2, were measured using western blot analysis...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29730855/chitosan-gelatin-scaffolds-support-bone-regeneration
#10
Anthie Georgopoulou, Fotios Papadogiannis, Aristea Batsali, John Marakis, Kalliopi Alpantaki, Aristides G Eliopoulos, Charalampos Pontikoglou, Maria Chatzinikolaidou
Chitosan/Gelatin (CS:Gel) scaffolds were fabricated by chemical crosslinking with glutaraldehyde or genipin by freeze drying. Both crosslinked CS:Gel scaffold types with a mass ratio of 40:60% form a gel-like structure with interconnected pores. Dynamic rheological measurements provided similar values for the storage modulus and the loss modulus of the CS:Gel scaffolds when crosslinked with the same concentration of glutaraldehyde vs. genipin. Compared to genipin, the glutaraldehyde-crosslinked scaffolds supported strong adhesion and infiltration of pre-osteoblasts within the pores as well as survival and proliferation of both MC3T3-E1 pre-osteoblastic cells after 7 days in culture, and human bone marrow mesenchymal stem cells (BM-MSCs) after 14 days in culture...
May 5, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29728612/identification-of-the-effector-domain-of-biglycan-that-facilitates-bmp-2-osteogenic-function
#11
Prapaporn Jongwattanapisan, Masahiko Terajima, Patricia A Miguez, William Querido, Hideaki Nagaoka, Noriko Sumida, Elizabeth Grace Gurysh, Kristy M Ainslie, Nancy Pleshko, Lalith Perera, Mitsuo Yamauchi
We have reported that recombinant biglycan (BGN) core protein accelerates bone formation in vivo by enhancing bone morphogenetic protein (BMP)-2 function. The purpose of the present study was to identify the specific domain ("effector") within the BGN core protein that facilitates BMP-2 osteogenic function. Thus, we generated various recombinant and synthetic peptides corresponding to several domains of BGN, and tested their effects on BMP-2 functions in vitro. The results demonstrated that the leucine-rich repeats 2-3 domain (LRR2-3) of BGN significantly enhanced the BMP-2 induced Smad1/5/9 phosphorylation, osteogenic gene expression, and alkaline phosphatase activity in myogenic C2C12 cells...
May 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29725360/-ajuga-decumbens-stimulates-mesenchymal-stem-cell-differentiation-and-regenerates-cartilage-in-a-rabbit-osteoarthritis-model
#12
Yoko Sawada, Atsushi Sugimoto, Tomohiro Osaki, Yoshiharu Okamoto
In a previous study by our group, Ajuga decumbens extract (ADE) was demonstrated to decrease the number of osteoclasts in subchondral bone and to have a synergistic effect with glucosamine in improving cartilaginous injuries in a rabbit model of osteoarthritis. In the present study, a concentrate of the useful fraction of ADE, termed extra ADE (EADE), which includes higher concentrations of the active component 20-hydroxyecdysone, was evaluated for its efficacy to accelerate the healing of experimental cartilage injury...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29721618/square-prism-micropillars-improve-osteogenicity-of-poly-methyl-methacrylate-surfaces
#13
O Hasturk, M Ermis, U Demirci, N Hasirci, V Hasirci
Osteogenicity and osteointegration of materials is one of the key elements of the success of bone implants. Poly(methyl methacrylate) (PMMA) is the basic compound of bone cement and has been widely investigated for other orthopedic applications, but its poor osteointegration and the subsequent loosening of implant material limits its widespread use as bone implants. Micropillar features on substrate surfaces were recently reported to modulate cell behavior through alteration of cell morphology and promotion of osteogenesis...
May 2, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29721422/runx2-osterix-and-zinc-uptake-synergize-to-orchestrate-osteogenic-differentiation-and-citrate-containing-bone-apatite-formation
#14
Xuekun Fu, Yunyan Li, Tongling Huang, Zhiwu Yu, Kun Ma, Meng Yang, Qingli Liu, Haobo Pan, Huaiyu Wang, Junfeng Wang, Min Guan
Citrate is essential to biomineralization of the bone especially as an integral part of apatite nanocomposite. Citrate precipitate of apatite is hypothesized to be derived from mesenchymal stem/stromal cells (MSCs) upon differentiation into mature osteoblasts. Based on 13 C-labeled signals identified by solid-state multinuclear magnetic resonance analysis, boosted mitochondrial activity and carbon-source replenishment of tricarboxylic acid cycle intermediates coordinate to feed forward mitochondrial anabolism and deposition of citrate...
April 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/29708246/development-of-non-orthogonal-3d-printed-scaffolds-to-enhance-their-osteogenic-performance
#15
Diana R Fonseca, Rita Sobreiro-Almeida, Paula C Sol, Nuno M Neves
Three-dimensional (3D)-printed polycaprolactone (PCL)-based scaffolds have been extensively proposed for Tissue Engineering (TE) applications. Currently, the majority of the scaffolds produced are not representative of the complex arrangement of natural structures, since the internal morphologies follow an orthogonal and regular pattern. In order to produce scaffolds that more closely replicate the structure of the extracellular matrix (ECM) of tissues, herein both circular and sinusoidal scaffolds were fabricated and compared to their conventional orthogonal counterparts...
April 30, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29707538/insulin-like-growth-factor-axis-expression-in-dental-pulp-cells-derived-from-carious-teeth
#16
Hanaa Esa Alkharobi, Hasanain Al-Khafaji, James Beattie, Deirdre Ann Devine, Reem El-Gendy
The insulin-like growth factor (IGF) axis plays an important role in dental tissue regeneration and most components of this axis are expressed in human dental pulp cells (DPCs). In our previous study, we analyzed IGF axis gene expression in DPCs and demonstrated a novel role of IGF binding protein (IGFBP)-2 and -3 in coordinating mineralized matrix formation in differentiating DPCs. A more recent study from our laboratory partially characterized dental pulp stem cells from teeth with superficial caries (cDPCs) and showed that their potential to differentiate odontoblasts and/or into osteoblasts is enhanced by exposure to the mild inflammatory conditions characteristic of superficial caries...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29705656/humanization-of-bone-and-bone-marrow-in-an-orthotopic-site-reveals-new-potential-therapeutic-targets-in-osteosarcoma
#17
Ferdinand Wagner, Boris M Holzapfel, Jacqui A McGovern, Abbas Shafiee, Jeremy G Baldwin, Laure C Martine, Christoph A Lahr, Felix M Wunner, Thor Friis, Onur Bas, Melanie Boxberg, Peter M Prodinger, Ali Shokoohmand, Davide Moi, Roberta Mazzieri, Daniela Loessner, Dietmar W Hutmacher
BACKGROUND: Existing preclinical murine models often fail to predict effects of anti-cancer drugs. In order to minimize interspecies-differences between murine hosts and human bone tumors of in vivo xenograft platforms, we tissue-engineered a novel orthotopic humanized bone model. METHODS: Orthotopic humanized tissue engineered bone constructs (ohTEBC) were fabricated by 3D printing of medical-grade polycaprolactone scaffolds, which were seeded with human osteoblasts and embedded within polyethylene glycol-based hydrogels containing human umbilical vein endothelial cells (HUVECs)...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29702291/multilayered-coating-of-titanium-implants-promotes-coupled-osteogenesis-and-angiogenesis-in-vitro-and-in-vivo
#18
Weizhen Chen, Kui Xu, Bailong Tao, Liangliang Dai, Yonglin Yu, Caiyun Mu, Xinkun Shen, Yan Hu, Ye He, Kaiyong Cai
We used surface-modified titanium (Ti) substrates with a multilayered structure composed of chitosan-catechol (Chi-C), gelatin (Gel) and hydroxyapatite (HA) nanofibers, which were previously shown to improve osteogenesis, as a platform to investigate the interaction of osteogenesis and angiogenesis during bone healing. Combined techniques of Transwell co-culture, wound healing assay, enzyme linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blotting and immunohistochemical staining were used to evaluate adhesion, morphology and migration of adipose-derived mesenchymal stem cells (Ad-MSCs) and human umbilical vein endothelial cells (HUVECs) grown on different Ti substrates...
April 24, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29701213/influence-of-the-nanofiber-chemistry-and-orientation-of-biodegradable-poly-butylene-succinate-based-scaffolds-on-osteoblast-differentiation-for-bone-tissue-regeneration
#19
Francesco Cristofaro, Matteo Gigli, Nora Bloise, Honglin Chen, Giovanna Bruni, Andrea Munari, Lorenzo Moroni, Nadia Lotti, Livia Visai
Innovative nanofibrous scaffolds have attracted considerable attention in bone tissue engineering, due to their ability to mimic the hierarchical architecture of an extracellular matrix. Aiming at investigating how the polymer chemistry and fiber orientation of electrospun scaffolds (ES) based on poly(butylene succinate) (PBS) and poly(butylene succinate/diglycolate) (P(BS80BDG20)) affect human osteoblast differentiation, uniaxially aligned (a-) and randomly (r-) distributed nanofibers were produced. Although human osteoblastic SAOS-2 cells were shown to be viable and adherent onto all ES materials, a-P(BS80BDG20) exhibited the best performance both in terms of cellular phosphorylated focal adhesion kinase expression and in terms of alkaline phosphatase activity, calcified bone matrix deposition and quantitative gene expression of bone specific markers during differentiation...
April 27, 2018: Nanoscale
https://www.readbyqxmd.com/read/29699909/osteoblastic-differentiation-on-hydrogels-fabricated-from-ca-2-responsive-self-assembling-peptides-functionalized-with-bioactive-peptides
#20
Hiroshi Tsutsumi, Megumi Kawamura, Hisakazu Mihara
We recently developed an amphiphilic peptide, E1Y9 (Ac-E-YEYKYEYKY-NH2 ), that self-assembles into nanofibers and forms a hydrogel in the presence of Ca2+ ion. Four E1Y9-derivatives (E1Y9-ALK, E1Y9-DGR, E1Y9-PRG and E1Y9-RGD) were designed as conjugates of E1Y9 with bioactive peptide sequences named as ALK (ALKRQGRTLYGF), DGR (DGRDSVAYG), PRG (PRGDSGYRGDS) and RGD (RGDS), respectively, and stimulated osteoblast cells growth as well as differentiation. In this study, E1Y9/E1Y9-derivative mixed hydrogels were constructed to serve as scaffolds for osteoblastic differentiation of MC3T3-E1 cells...
April 18, 2018: Bioorganic & Medicinal Chemistry
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