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https://www.readbyqxmd.com/read/28225019/trpv1-deletion-impaired-fracture-healing-and-inhibited-osteoclast-and-osteoblast-differentiation
#1
Lin-Hai He, Meng Liu, Yang He, E Xiao, Lu Zhao, Ting Zhang, Hua-Qian Yang, Yi Zhang
Fracture healing, in which osteoclasts and osteoblasts play important roles, has drawn much clinical attention. Osteoclast deficiency or decreased osteoblast activity will impair fracture healing. TRPV1 is a member of the Ca(2+) permeable cation channel subfamily, and pharmacological inhibition of TRPV1 prevents ovariectomy-induced bone loss, which makes TRPV1 a potential target for osteoporosis. However, whether long term TRPV1 inhibition or TRPV1 deletion will affect the fracture healing process is unclear...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223547/cabozantinib-targets-bone-microenvironment-modulating-human-osteoclast-and-osteoblast-functions
#2
Marco Fioramonti, Daniele Santini, Michele Iuliani, Giulia Ribelli, Paolo Manca, Nicola Papapietro, Filippo Spiezia, Bruno Vincenzi, Vincenzo Denaro, Antonio Russo, Giuseppe Tonini, Francesco Pantano
Cabozantinib, a c-MET and vascular endothelial growth factor receptor 2 inhibitor, demonstrated to prolong progression free survival and improve skeletal disease-related endpoints in castration-resistant prostate cancer and in metastatic renal carcinoma. Our purpose is to investigate the direct effect of cabozantinib on bone microenvironment using a total human model of primary osteoclasts and osteoblasts.Osteoclasts were differentiated from monocytes isolated from healthy donors; osteoblasts were derived from human mesenchymal stem cells obtained from bone fragments of orthopedic surgery patients...
February 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28221217/interobserver-reproducibility-among-gynecologic-pathologists-in-diagnosing-heterologous-osteosarcomatous-component-in-gynecologic-tract-carcinosarcomas
#3
Ankur R Sangoi, Malti Kshirsagar, Andres A Roma, Andrew E Horvai, Mamatha Chivukula, Lora H Ellenson, Oluwole Fadare, Ann K Folkins, Karuna Garg, Krisztina Hanley, Teri A Longacre, Jacqueline Haas, W Glenn McCluggage, Jesse K McKenney, Marisa R Nucci, Esther Oliva, Kay J Park, Vinita Parkash, Charles M Quick, Joseph T Rabban, Joanne K L Rutgers, Robert Soslow, Russell Vang, Anna Yemelyanova, Charles Zaloudek, Andrew H Beck
Distinguishing hyalinized stroma from osteoid production by a heterologous osteosarcomatous component can be challenging in gynecologic tract carcinosarcomas. As heterologous components in a carcinosarcoma may have prognostic and therapeutic implications, it is important that these are recognized. This study examines interobserver reproducibility among gynecologic pathologists in the diagnosis of osteosarcomatous components, and its correlation with expression of the novel antibody SATB2 (marker of osteoblastic differentiation) in these osteosarcomatous foci...
February 17, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28221139/how-do-stones-form-is-unification-of-theories-on-stone-formation-possible
#4
Victoria Y Bird, Saeed R Khan
There are two basic pathways for formation of calcium based kidney stones. Most idiopathic calcium oxalate (CaOx) stones are formed in association with sub-epithelial plaques of calcium phosphate (CaP), known as Randall's plaques, on renal papillary surfaces. Crystal formation and retention within the terminal collecting ducts, the ducts of Bellini, leading to the formation of Randall's plugs, is the other pathway. Both pathways require supersaturation leading to crystallization, regulated by various crystallization modulators produced in response to changing urinary conditions...
January 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28220845/heterogeneous-nuclear-ribonucleoprotein-a1-regulates-rhythmic-synthesis-of-mouse-nfil3-protein-via-ires-mediated-translation
#5
Hyo-Jin Kim, Hwa-Rim Lee, Ji-Young Seo, Hye Guk Ryu, Kyung-Ha Lee, Do-Yeon Kim, Kyong-Tai Kim
Nuclear factor, interleukin 3, regulated (Nfil3, also known as E4 Promoter-Binding Protein 4 (E4BP4)) protein is a transcription factor that binds to DNA and generally represses target gene expression. In the circadian clock system, Nfil3 binds to a D-box element residing in the promoter of clock genes and contributes to their robust oscillation. Here, we show that the 5'-untranslated region (5'-UTR) of Nfil3 mRNA contains an internal ribosome entry site (IRES) and that IRES-mediated translation occurs in a phase-dependent manner...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220828/rspo3-lgr4-regulates-osteogenic-differentiation-of-human-adipose-derived-stem-cells-via-erk-fgf-signalling
#6
Min Zhang, Ping Zhang, Yunsong Liu, Longwei Lv, Xiao Zhang, Hao Liu, Yongsheng Zhou
The four R-spondins (RSPOs) and their three related receptors, LGR4, 5 and 6, have emerged as a major ligand-receptor system with critical roles in development and stem cell survival. However, the exact roles of the RSPO-LGR system in osteogenesis remain largely unknown. In the present study, we showed that RSPO3-shRNA increased the osteogenic potential of human adipose-derived stem cells (hASCs) significantly. Mechanistically, we demonstrated that RSPO3 is a negative regulator of ERK/FGF signalling. We confirmed that inhibition of the ERK1/2 signalling pathway blocked osteogenic differentiation in hASCs, and the increased osteogenic capacity observed after RSPO3 knockdown in hASCs was reversed by inhibition of ERK signalling...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219807/setd2-is-associated-with-strontium-induced-bone-regeneration
#7
Jia Xiaoshi, Long Qiaoyun, Richard J Miron, Yin Chengcheng, Wei Yan, Zhang Yufeng, Wu Min
: Strontium Ranelate has been utilized as a preventative treatment option for osteoporosis with the release of Sr ions having a direct effect on preventing osteoclast activation and promoting osteoblast differentiation. Previously our group has prepared and characterized a porous Sr-mesoporous bioactive glass (Sr-MBG) scaffold demonstrating its ability to enhance new bone formation when compared to MBG alone. The goal of the present study was to elucidate the bone-inducing properties of Sr by utilizing RNA-seq on in vivo tissue samples to investigate potential target genes responsible for Sr-induced new bone formation...
February 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28218908/cell-matrix-signals-specify-bone-endothelial-cells-during-developmental-osteogenesis
#8
Urs H Langen, Mara E Pitulescu, Jung Mo Kim, Rocio Enriquez-Gasca, Kishor K Sivaraj, Anjali P Kusumbe, Amit Singh, Jacopo Di Russo, M Gabriele Bixel, Bin Zhou, Lydia Sorokin, Juan M Vaquerizas, Ralf H Adams
Blood vessels in the mammalian skeletal system control bone formation and support haematopoiesis by generating local niche environments. While a specialized capillary subtype, termed type H, has been recently shown to couple angiogenesis and osteogenesis in adolescent, adult and ageing mice, little is known about the formation of specific endothelial cell populations during early developmental endochondral bone formation. Here, we report that embryonic and early postnatal long bone contains a specialized endothelial cell subtype, termed type E, which strongly supports osteoblast lineage cells and later gives rise to other endothelial cell subpopulations...
February 20, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28218774/osteoblast-differentiation-of-rabbit-adipose-derived-stem-cells-by-polyethylenimine-mediated-bmp-2-gene-transfection-in-vitro
#9
Q F Wang, Y Huang, G C He, H S Wang, Z H Chen, X H Cai, Y H Xie, Q Liu
The aim of this study was to examine the efficiency of polyethylenimine-mediated transfection of the human bone morphogenetic protein-2 (BMP-2) gene into rabbit adipose-derived stem cells (ADSCs), and its effect on osteoblast differentiation. Adipose tissue was isolated from the necks of adult Japanese white rabbits and cultured in vitro to obtain ADSCs. Gene delivery of BMP-2 was mediated by polyethylenimine and stable transformants were selected by G-418. The expression of BMP-2 mRNA was confirmed by reverse transcription-polymerase chain reaction, and of the BMP-2 protein by ELISA...
February 16, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28218496/functional-analysis-of-bioactivated-surfaces
#10
Selgai Haidari, Marko Boskov, Ulrike Schillinger, Klaus-Dietrich Wolff, Christian Plank, Andreas Kolk
Common scaffold surfaces, such as titanium, can have side effects, e.g., infections, cytotoxicity, impaired osseointegration or low regeneration rates for bone tissue. These effects lead to poor implant integration or even implant loss. Therefore, bioactive implants are promising instruments in tissue regeneration. Osteoinductive elements, such as growth factors and anti-infectives, support wound healing and bone growth and thereby enable faster osseointegration, even in elderly patients. In this study, titanium surfaces were coated with a poly-(D,L-lactide) (PDLLA) layer containing different concentrations of copolymer-protected gene vectors (COPROGs) to locally provide bone morphogenetic protein-2 (BMP-2) or activated anti-infective agents, such as chlorhexidine gluconate, triclosan and metronidazole, to prevent peri-implantitis...
February 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28218482/osteoblastic-differentiation-of-periodontal-ligament-stem-cells-on-non-stoichiometric-calcium-phosphate-and-titanium-surfaces
#11
L Winning, L Robinson, A R Boyd, I A El Karim, F T Lundy, B J Meenan
Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1+ve periodontal ligament stem cells (PDLSCs) to three well-characterised biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nano-topography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi)...
February 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28218468/igf-1-receptor-expression-on-circulating-osteoblast-progenitor-cells-predicts-tissue-based-bone-formation-rate-and-response-to-teriparatide-in-premenopausal-women-with-idiopathic-osteoporosis
#12
Adi Cohen, Stavroula Kousteni, Brygida Bisikirska, Jayesh G Shah, J Sanil Manavalan, Robert R Recker, Joan Lappe, David W Dempster, Hua Zhou, Donald J McMahon, Mariana Bucovsky, Mafo Kamanda-Kosseh, Julie Stubby, Elizabeth Shane
We have previously reported that premenopausal women with idiopathic osteoporosis (IOP) have profound microarchitectural deficiencies and heterogeneous bone remodeling. Those with the lowest bone formation rate have higher baseline serum IGF-1 levels and less robust response to teriparatide. Because IGF-1 stimulates bone formation and is critical for teriparatide action on osteoblasts, these findings suggest a state of IGF-1 resistance in some IOP women. To further investigate the hypothesis that osteoblast and IGF-1 related mechanisms mediate differential responsiveness to teriparatide in IOP, we studied circulating osteoblast progenitor (COP) cells and their IGF-1 receptor (IGF-1R) expression...
February 20, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28218329/dopamine-assisted-fixation-of-drug-loaded-polymeric-multilayers-to-osteoarticular-implants-for-tuberculosis-therapy
#13
Dan Li, Litao Li, Yunlong Ma, Yaping Zhuang, Dawei Li, Hong Shen, Xing Wang, Fei Yang, Yuanzheng Ma, Decheng Wu
Currently, the major issues in the treatment of osteoarticular tuberculosis (TB) after implant placement are low drug concentration at the infected focus and drug resistance resulting from the long-term chemotherapy. The application of drug-loaded polymeric multilayers on implantable devices offers a promising solution to the problems. Herein, a poly(ethylene glycol)-based hydrogel film embedded with isoniazid (INH)-loaded alginate microparticles was fixed to Ti implants via adhesive polydopamine, subsequently capped by poly(lactic-co-glycolic acid) membranes for the sustained and localized delivery of the anti-TB drug...
February 20, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28218305/interaction-of-cutibacterium-%C3%A2-formerly-propionibacterium-acnes-with-bone-cells-a-step-toward-understanding-bone-and-joint-infection-development
#14
Guillaume Ghislain Aubin, Marc Baud'huin, Jean-Philippe Lavigne, Régis Brion, François Gouin, Didier Lepelletier, Cédric Jacqueline, Dominique Heymann, Karim Asehnoune, Stéphane Corvec
Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by live C. acnes. The whole genome sequence of six isolates of this collection was analyzed. CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28217533/role-of-odanacatib-in-reducing-bone-loss-due-to-endodontic-disease-an-overview
#15
REVIEW
Rachana Bahuguna, Atul Jain, Suleman Abbas Khan, M S Arvind
AIMS AND OBJECTIVES: Through a comprehensive literature review, this article provides an overview of the potential role of odanacatib (ODN) in reducing bone loss due to endodontic disease. MATERIALS AND METHODS: A literature review was performed in PubMed Central, MEDLINE, Cochrane Library, and EBSCO databases. The articles identified included those published between 2002 and 2016. Based on the predetermined inclusion and exclusion criteria, out of 237 articles found, 50 were selected for this review...
December 2016: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28217387/histological-assessment-of-porous-custom-made-hydroxyapatite-implants-6-months-and-2-5-years-after-cranioplasty
#16
Hajime Ono, Taigen Sase, Yuichiro Tanaka, Hiroshi Takasuna
BACKGROUND: In cranial reconstruction, the features of artificial bone differ. Custom-made porous hydroxyapatite (HAp) implants for cranioplasty have been used all over the world because of their good cosmetic, biocompatibility, and osteoconductive properties. Surgical techniques were analyzed, and histological assessment of new bone formation in the hydroxyapatite was performed. METHODS: Over a 6-year time period, 41 patients underwent cranioplasty using a custom-made three-dimensional hybrid pore structured hydroxyapatite (3DHPoHAp) implant...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28216889/osteoblasts-proliferation-and-differentiation-stimulating-activities-of-the-main-components-of-epimedii-folium
#17
Mingming Liu, Haiyan Xu, Yong Ma, Jian Cheng, Zhen Hua, Guicheng Huang
BACKGROUND: Osteoporosis is a disease of bones that leads to an increased risk of fracture. Epimedii Folium is commonly used for treating bone fractures and joint diseases for thousands of years in China. METHODS: This study was aimed to screen active components, which might have the potency to stimulate osteoblasts proliferation and differentiation in Epimedii Folium. An HPLC method was established to analyze the main components in Epimedii Folium. The MTT and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28216630/smurf2-regulates-bone-homeostasis-by-disrupting-smad3-interaction-with-vitamin-d-receptor-in-osteoblasts
#18
Zhan Xu, Matthew B Greenblatt, Guang Yan, Heng Feng, Jun Sun, Sutada Lotinun, Nicholas Brady, Roland Baron, Laurie H Glimcher, Weiguo Zou
Coordination between osteoblasts and osteoclasts is required for bone health and homeostasis. Here we show that mice deficient in SMURF2 have severe osteoporosis in vivo. This low bone mass phenotype is accompanied by a pronounced increase in osteoclast numbers, although Smurf2-deficient osteoclasts have no intrinsic alterations in activity. Smurf2-deficient osteoblasts display increased expression of RANKL, the central osteoclastogenic cytokine. Mechanistically, SMURF2 regulates RANKL expression by disrupting the interaction between SMAD3 and vitamin D receptor by altering SMAD3 ubiquitination...
February 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28216602/selenium-and-tellurium-based-antioxidants-for-modulating-inflammation-and-effects-on-osteoblastic-activity
#19
Xi Lu, Gemma Mestres, Vijay Pal Singh, Pedram Effati, Jia-Fei Poon, Lars Engman, Marjam Karlsson Ott
Increased oxidative stress plays a significant role in the etiology of bone diseases. Heightened levels of H2O2 disrupt bone homeostasis, leading to greater bone resorption than bone formation. Organochalcogen compounds could act as free radical trapping agents or glutathione peroxidase mimetics, reducing oxidative stress in inflammatory diseases. In this report, we synthesized and screened a library of organoselenium and organotellurium compounds for hydrogen peroxide scavenging activity, using macrophagic cell lines RAW264...
February 14, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28215635/mechanical-stimulation-promote-the-osteogenic-differentiation-of-bone-marrow-stromal-cells-through-epigenetic-regulation-of-sonic-hedgehog
#20
Chuandong Wang, Shengzhou Shan, Chenglong Wang, Jing Wang, Jiao Li, Guoli Hu, Kerong Dai, Qingfeng Li, Xiaoling Zhang
Mechanical unloading leads to bone loss and disuse osteoporosis partly due to impaired osteoblastogenesis of bone marrow stromal cells (BMSCs). However, the underlying molecular mechanisms of this phenomenon are not fully understood. In this study, we demonstrated that cyclic mechanical stretch (CMS) promotes osteoblastogenesis of BMSCs both in vivo and in vitro. Besides, we found that Hedgehog (Hh) signaling pathway was activated in this process. Inhibition of which by either knockdown of Sonic hedgehog (Shh) or treating BMSCs with Hh inhibitors attenuated the osteogenic effect of CMS on BMSCs, suggesting that Hh signaling pathway acts as an endogenous mediator of mechanical stimuli on BMSCs...
February 16, 2017: Experimental Cell Research
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