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https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#1
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944904/triptolide-inhibits-proliferation-differentiation-and-induces-apoptosis-of-osteoblastic-mc3t3%C3%A2-e1-cells
#2
Wei Ji, Shijia Liu, Xia Zhao, Yunke Guo, Sha Xia, Yueyang Lu, Menyun Yin, Xiao Xu
Ankylosing spondylitis (AS) is characterized by the formation of bony spurs. Treatment of the resulting ankylosis, excessive bone formation and associated functional impairment, remain the primary therapeutic aims in research regarding this condition. Triptolide is the primary active component of the perennial vine Tripterygium wilfordii Hook. f., and has previously been demonstrated to exert anti‑tumor activities including inhibition of cell growth and the induction of apoptosis, however, the effect of triptolide on osteoblasts remains to be elucidated...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944833/low-doses-of-co-nanoparticles-induce-death-and-regulate-osteogenic-differentiation-in-mg%C3%A2-63-cells
#3
Qinglin Han, Fan Liu
The aim of the present study was to investigate the effects of cobalt nanoparticles (CoNPs) on the proliferation and differentiation of human osteoblasts in vitro, and to investigate the molecular mechanisms via which CoNPs affect proliferation and differentiation of osteoblasts. The MG‑63 human osteoblast cell line was treated with different concentrations of CoNPs for 12 to 48 h in vitro. At each time point, cell morphology was observed and an MTT assay was performed to assess cell viability. Alkaline phosphatase (ALP), osteocalcin (BGLAP), collagen I (COL I), osteoprotegerin (OPG) and receptor activator of nuclear factor κ‑B ligand mRNA expression levels, and ALP, BGLAP and COL protein expression levels, were assessed by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944822/investigation-of-crucial-genes-and-micrornas-in-conventional-osteosarcoma-using-gene-expression-profiling-analysis
#4
Chuangang Peng, Qi Yang, Bo Wei, Baoming Yuan, Yong Liu, Yuxiang Li, Dawer Gu, Guochao Yin, Bo Wang, Dehui Xu, Xuebing Zhang, Daliang Kong
The present study aimed to screen potential genes associated with conventional osteosarcoma (OS) and obtain further information on the pathogenesis of this disease. The microarray dataset GSE14359 was downloaded from the Gene Expression Omnibus. A total of 10 conventional OS samples and two non‑neoplastic primary osteoblast samples in the dataset were selected to identify the differentially expressed genes (DEGs) using the Linear Models for Microarray Data package. The potential functions of the DEGs were predicted using Gene Ontology (GO) and pathway enrichment analyses...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944766/magnesium-oxide-nanoparticle-loaded-polycaprolactone-composite-electrospun-fiber-scaffolds-for-bone-soft-tissue-engineering-applications-in-vitro-and-in-vivo-evaluation
#5
Ajay Suryavanshi, Kunal Khanna, K R Sindhu, Jayesh Bellare, Rohit Srivastava
The objective of the present investigation was to assess the potential of magnesium oxide nanoparticle (MgO NP)-loaded electrospun polycaprolactone (PCL) polymer composites as a bone-soft tissue engineering scaffold. MgO NPs were synthesized using a hydroxide precipitation sol-gel method and characterized using field emission gun-scanning electron microscopy/energy-dispersive x-ray spectroscopy (FEG-SEM/EDS), field emission gun-transmission electron microscopy (FEG-TEM), and x-ray diffraction (XRD) analysis...
September 25, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28944287/detection-of-pentosidine-cross-links-in-cell-secreted-decellularized-matrices-using-time-resolved-fluorescence-spectroscopy
#6
Debika Mitra, Hussain Fatakdawala, Michael Nguyen-Truong, Amy Creecy, Jeffry Nyman, Laura Marcu, J Kent Leach
Hyperglycemia-mediated, nonenzymatic collagen cross-links such as pentosidine (PENT) can have deleterious effects on cellular interactions with the extracellular matrix (ECM). Present techniques to quantify PENT are limited, motivating the need for improved methods to study the accumulation and contribution of PENT toward diabetic clinical challenges such as impaired bone healing. Current methods for studying PENT are destructive, laborious, and frequently employ oversimplified collagen films that lack the complexity of the native ECM...
September 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28944087/exercise-induced-irisin-in-bone-and-systemic-irisin-administration-reveal-new-regulatory-mechanisms-of-bone-metabolism
#7
Jin Zhang, Paloma Valverde, Xiaofang Zhu, Dana Murray, Yuwei Wu, Liming Yu, Hua Jiang, Michel M Dard, Jin Huang, Zhiwei Xu, Qisheng Tu, Jake Chen
Irisin is a polypeptide hormone derived from the proteolytic cleavage of fibronectin-type III domain-containing 5 (FNDC5) protein. Once released to circulation upon exercise or cold exposure, irisin stimulates browning of white adipose tissue (WAT) and uncoupling protein 1 (UCP1) expression, leading to an increase in total body energy expenditure by augmented UCP1-mediated thermogenesis. It is currently unknown whether irisin is secreted by bone upon exercise or whether it regulates bone metabolism in vivo...
2017: Bone Research
https://www.readbyqxmd.com/read/28944060/one-ion-to-rule-them-all-combined-antibacterial-osteoinductive-and-anticancer-properties-of-selenite-incorporated-hydroxyapatite
#8
Vuk Uskoković, Maheshwar Adiraj Iyer, Victoria M Wu
Although hydroxyapatite (HAp) has been doped with dozens of different ions, the quest for an ion imparting a combination of properties conducive to bone healing is still ongoing. Because of its protean potency and the similarity in size and shape to the phosphate tetrahedron, selenite ion presents a natural ionic substitute in HAp. The incorporation of selenite into synthetic HAp using two different methods - co-precipitation and ion-exchange sorption - was studied for its effect on crystal properties and on a triad of biological responses: antibacterial, anticancer and osteoinductive...
2017: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/28942160/gold-nanoconjugates-reinforce-the-potency-of-conjugated-cisplatin-and-doxorubicin
#9
Sana Iram, Manaal Zahera, Salman Khan, Imran Khan, Asad Syed, Abu Ayoobul Ansary, Fuad Ameen, Omar H M Shair, Mohd Sajid Khan
Osteosarcoma or osteogenic sarcoma is the most common and prevalent cancerous tumor of bone and occurs especially in children and teens. Recent treatment strategy includes a combination of both chemotherapy and surgeries. Although, the use of single drug-based chemotherapy treatment remains unsatisfactory. Therefore, combinatorial therapy has emerged as a potential strategy for treatment with limited side- effects. Here, we evaluated the combinatorial anticancerous effect of cisplatin (CIS) and doxorubicin (DOX) bioconjugated bromelain encapsulated gold nanoparticles (B-AuNPs conjugated CIS and DOX) in the treatment of osteosarcoma...
September 7, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28942121/ex-vivo-construction-of-human-primary-3d-networked-osteocytes
#10
Qiaoling Sun, Saba Choudhary, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Y Lee
A human bone tissue model was developed by constructing ex vivo the 3D network of osteocytes via the biomimetic assembly of primary human osteoblastic cells with 20-25μm microbeads and subsequent microfluidic perfusion culture. The biomimetic assembly: (1) enabled 3D-constructed cells to form cellular network via processes with an average cell-to-cell distance of 20-25μm, and (2) inhibited cell proliferation within the interstitial confine between the microbeads while the confined cells produced extracellular matrix (ECM) to form a mechanically integrated structure...
September 20, 2017: Bone
https://www.readbyqxmd.com/read/28941279/suppressing-angiogenesis-regulates-the-irradiation-induced-stimulation-on-osteoclastogenesis-in-vitro
#11
Ling Tong, Guoying Zhu, Jianping Wang, Ruilian Sun, Feilong He, Jianglong Zhai
Ionizing radiation-induced bone loss is a potential health concern in radiotherapy, occupational exposure and astronauts. Although impaired bone vasculature and reduced proliferation of bone-forming osteoblasts has been implicated in this process, it has not been clearly characterized that whether radiation affects the growth of bone-resorbing osteoclasts. The molecular crosstalk between different cell populations in the skeletal system has not yet been elucidated in detail, especially between the increased bone resorption at early stage of post-irradiation and bone marrow-derived endothelial progenitor cells (BM-EPCs)...
September 23, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28941043/cocr-enriched-medium-modulates-integrin-based-downstream-signaling-and-requires-a-set-of-inflammatory-genes-reprograming-in-vitro
#12
Célio J C Fernandes, Fabio Bezerra, Maiara das D do Carmo, Georgia Feltran, Mariana C Rossi, Rodrigo A da Silva, Pedro de M Padilha, Willian F Zambuzzi
Significant health concerns have been raised by the high levels of Cr and Co ions into whole blood as resulted of corrosion process released from biomedical implants, but very little is known about their biological behavior in governing cell metabolism. Thus, we prompted to address this issue by exploring the effects of CoCr enriched medium on both fibroblast and pre-osteoblast (pre-Ob) cells. Firstly, we showed there is a significant difference in Co and Cr releasing dependent on engineered surface, it being even more released in dual acid-etching treating surface (named w/DAE) than the machined surfaces (named wo/DAE)...
September 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28940469/cytoprotective-effects-of-xanthohumol-against-methylglyoxal-induced-cytotoxicity-in-mc3t3-e1-osteoblastic-cells
#13
Kwang Sik Suh, Suk Chon, Eun Mi Choi
Methylglyoxal (MG) has been suggested to be a major source of intracellular reactive carbonyl compounds, and has been implicated in increasing the levels of advanced glycation end products in age-related diseases. Xanthohumol is a prenylated flavonoid found in hops (Humulus lupulus) and beer. In the present study, we investigated the effects of xanthohumol on MG-induced cytotoxicity in osteoblastic MC3T3-E1 cells. Xanthohumol attenuated MG-induced cytotoxicity, as evidenced by improved cell viability, and prevented MG-induced MG-protein adducts, inflammatory cytokines, reactive oxygen species and mitochondrial superoxide production...
September 20, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28940410/mechanisms-of-bone-destruction-in-multiple-myeloma
#14
REVIEW
E Terpos, D Christoulas, M Gavriatopoulou, M A Dimopoulos
Osteolytic bone disease is a frequent complication of multiple myeloma, resulting in skeletal complications that are a significant cause of morbidity and mortality. It is the result of an increased activity of osteoclasts, which is not followed by reactive bone formation by osteoblasts. Recent studies have revealed novel molecules and pathways that are implicated in osteoclast activation and osteoblast inhibition. Among them, the most important include the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin pathway, the macrophage inflammatory proteins and the activin-A that play a crucial role in osteoclast stimulation in myeloma, while the wingless-type (Wnt) signalling inhibitors (sclerostin and dickkopf-1) along with the growth factor independence-1 are considered the most important factors for the osteoblast dysfunction of myeloma patients...
September 21, 2017: European Journal of Cancer Care
https://www.readbyqxmd.com/read/28940008/p21-waf1-cip1-depletion-promotes-dexamethasone-induced-apoptosis-in-osteoblastic-mc3t3-e1-cells-by-inhibiting-the-nrf2-ho-1-pathway
#15
Dandan Han, Jian Gao, Xiaolong Gu, Jan Georg Hengstler, Limei Zhang, Muhammad Shahid, Tariq Ali, Bo Han
Dexamethasone (Dex), a glucocorticoid with strong anti-inflammatory and immunosuppressive activities, has been shown to exhibit marked cytotoxicity and apoptosis in osteoblasts, but the underlying mechanisms have not yet been comprehensively investigated. P21(Waf1/Cip1) (p21) plays a critical role in the regulation of cell cycle progression and apoptosis. The present study aims to investigate the role of p21 in Dex-induced apoptosis in osteoblastic MC3T3-E1 cells, and to explore its mechanisms. Results demonstrated that Dex-induced apoptosis decreased the phosphorylation of Akt in a concentration-dependent manner...
September 22, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28939921/pathophysiology-and-treatment-of-cardiovascular-disease-in-pediatric-chronic-kidney-disease
#16
REVIEW
Nadine Khouzam, Katherine Wesseling-Perry
Life expectancy in patients with all stages of chronic kidney disease (CKD) falls far short of that in the general population. Cardiovascular disease is the leading cause of mortality in pediatric patients with CKD. In contrast to the intimal atherosclerotic lesions that characterize cardiovascular disease in the general population, vascular endothelial dysfunction, medial arterial calcification, and cardiac dysfunction contribute to cardiovascular pathological conditions in CKD. The pathogenesis of these lesions, the origins of which can be identified in the absence of traditional cardiovascular risk factors, is incompletely understood...
September 22, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28939526/inhibition-of-the-tgf-%C3%AE-1-smad-signaling-pathway-protects-against-cartilage-injury-and-osteoarthritis-in-a-rat-model
#17
Yong-Jian Wang, Min Shen, Shan Wang, Xin Wen, Xin-Rui Han, Zi-Feng Zhang, Hui Li, Fei Wang, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
Transforming growth factor-β1 (TGF-β1) in osteoblastic cells triggers pathological changes observed in osteoarthritis (OA). Thus, we hypothesized that the inhibition of the TGF-β1/Smad signaling pathway could act to protect against cartilage injury in rats with OA. After establishment of rat models induced by papain and L-cysteine, rats were subsequently assigned into the TβRI inhibitors, normal control (NC) and OA model groups. RT-qPCR and western blotting were performed to determine mRNA and protein expressions of TGFβ1, Smad2, Smad3 and Activin receptor-like kinase 5 (ALK5), respectively...
September 19, 2017: Life Sciences
https://www.readbyqxmd.com/read/28939019/anti-inflammatory-effects-of-traditional-mixed-extract-of-medicinal-herbs-memh-on-monosodium-urate-crystal-induced-gouty-arthritis
#18
Ju-Suk Nam, Supriya Jagga, Ashish Ranjan Sharma, Joon-Hee Lee, Jong Bong Park, Jun-Sub Jung, Sang-Soo Lee
Korean oriental medicine prescription is widely used for the treatment of gouty diseases. In the present study, we investigated anti-inflammatory effects of modified Korean herbal formulation, mixed extract of medicinal herbs (MEMH), and its modulatory effects on inflammatory mediators associated with gouty arthritis. Both in vitro and in vivo studies were carried out to assess the anti-inflammatory efficacy of MEMH on monosodium urate (MSU) crystals-induced gouty inflammation. MSU crystals stimulated human chondrosarcoma cell line, SW1353, and human primary chondrocytes were treated with MEMH in vitro...
August 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28938631/microrna-455-targets-cullin-3-to-activate-nrf2-signaling-and-protect-human-osteoblasts-from-hydrogen-peroxide
#19
Dawei Xu, Hao Zhu, Chengniu Wang, Xinhui Zhu, Genxiang Liu, Chu Chen, Zhiming Cui
Over-production of hydrogen peroxide (H2O2) will lead to human osteoblast dysfunction and apoptosis, causing progression of osteoporosis and osteonecrosis. NF-E2-related factor 2 (Nrf2) is a well-characterized anti-oxidant signaling. Cullin 3 (Cul3) ubiquitin E3 ligase dictates Nrf2 degradation. We demonstrate that microRNA-455 ("miR-455") is a putative Cul3-targeting microRNA. Forced-expression of miR-455 in both hFOB1. 19 osteoblast cell line and primary human osteoblasts induced Cul3 degradation and Nrf2 protein stabilization, which led to subsequent transcription of ARE (anti-oxidant response element)-dependent genes (NQO1, HO1 and GCLC)...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938572/osthole-inhibits-bone-metastasis-of-breast-cancer
#20
Chunyu Wu, Zhenping Sun, Baofeng Guo, Yiyi Ye, Xianghui Han, Yuenong Qin, Sheng Liu
Bone is one of the most common sites for breast cancer metastasis, which greatly contributes to patient morbidity and mortality. Osthole, a major extract from Cnidium monnieri (L.), exhibits many biological and pharmacological activities, however, its potential as a therapeutic agent in the treatment of breast cancer bone metastases remain poorly understood. In this study, we set out to investigate whether osthole could inhibit breast cancer metastasis to bone in mice and clarified the potential mechanism of this inhibition...
August 29, 2017: Oncotarget
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