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Transcription factor tissue engineering

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https://www.readbyqxmd.com/read/28928909/transplanting-embryonic-stem-cells-onto-damaged-human-corneal-endothelium
#1
Charles Hanson, Arsaell Arnarsson, Thorir Hardarson, Ann Lindgård, Mandana Daneshvarnaeini, Catarina Ellerström, Anita Bruun, Ulf Stenevi
AIM: To investigate whether human embryonic stem cells (hESCs) could be made to attach, grow and differentiate on a human Descemet's membrane (DM). METHODS: Spontaneously differentiated hESCs were transferred onto a human corneal button with the endothelial layer removed using ocular sticks. The cells were cultured on a DM for up to 15 d. The genetically engineered hESC line expressed green fluorescent protein, which facilitated identification during the culture experiments, tissue preparation, and analysis...
August 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/28928460/molecular-dissection-of-transcriptional-reprogramming-of-steviol-glycosides-synthesis-in-leaf-tissue-during-developmental-phase-transitions-in-stevia-rebaudiana-bert
#2
Gopal Singh, Gagandeep Singh, Pradeep Singh, Rajni Parmar, Navgeet Paul, Radhika Vashist, Mohit Kumar Swarnkar, Ashok Kumar, Sanatsujat Singh, Anil Kumar Singh, Sanjay Kumar, Ram Kumar Sharma
Stevia is a natural source of commercially important steviol glycosides (SGs), which share biosynthesis route with gibberellic acids (GAs) through plastidal MEP and cytosolic MVA pathways. Ontogeny-dependent deviation in SGs biosynthesis is one of the key factor for global cultivation of Stevia, has not been studied at transcriptional level. To dissect underlying molecular mechanism, we followed a global transcriptome sequencing approach and generated more than 100 million reads. Annotation of 41,262 de novo assembled transcripts identified all the genes required for SGs and GAs biosynthesis...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28921443/polyester-based-nanoparticles-for-the-encapsulation-of-monoclonal-antibodies
#3
Flávia Sousa, Pedro Fonte, Andreia Cruz, Patrick J Kennedy, Inês Mendes Pinto, Bruno Sarmento
Aliphatic polyesters have been widely explored for biomedical applications (e.g., drug delivery systems, biomedical devices, and tissue engineering). Recently, polyesters have been used in nanoparticle formulations for the controlled release of monoclonal antibodies (mAbs) for the enhanced efficacy of antibody-based therapy. Polyester-based nanoparticles for mAb delivery provide decreased antibody dosage, increased antibody stability and protection and longer therapeutic action, ultimately translating to an increased therapeutic index...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28903624/repair-of-calvarial-bone-defect-using-jarid1a-knockdown-bone-mesenchymal-stem-cells-in-rats
#4
Yuan Deng, Tao Guo, Jipeng Li, Li Guo, Ping Gu, Xianqun Fan
Histone methylation is regarded as an important epigenetic event during stem cell differentiation. Jumonji AT-rich interactive domain 1A (Jarid1a) is a histone demethylase that specifically catalyzes demethylation of dimethyl or trimethyl histone H3K4me3, which is normally associated with transcriptionally active genes. Runt-related transcription factor 2 (Runx2) has been identified as a key transcription factor in the early stage of osteogenesis. A better understanding of this epigenetic mechanism that governs osteogenic differentiation of bone mesenchymal stem cells (BMSCs) can provide new insights into BMSCs based bone tissue engineering...
September 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28879758/light-controlled-bmsc-sheet-implant-complexes-with-improved-osteogenesis-via-an-lrp5-%C3%AE-catenin-runx2-regulatory-loop
#5
Zhiwei Jiang, Huiming Wang, Ke Yu, Yuting Feng, Ying Wang, Tingben Huang, Kaichen Lai, Yue Xi, Guoli Yang
The combination of bone marrow mesenchymal stem cell (BMSC) sheets and titanium implants (BMSC sheet-implant complexes) can accelerate osseointegration. However, methods of fabricating BMSC sheet-implant complexes are quite limited, and the survival of BMSC sheet-implant complexes is one of the key barriers. Here, we show that a light-controlled fabricating system can generate less injured BMSC sheet-implant complexes with improved viability and osteogenesis, and non-invasive monitoring of viability of BMSC sheet-implant complexes using lentiviral delivery system is feasible...
September 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28864123/effects-of-glucose-lactate-and-basic-fgf-as-limiting-factors-on-the-expansion-of-human-induced-pluripotent-stem-cells
#6
Ikki Horiguchi, Yusuke Urabe, Keiichi Kimura, Yasuyuki Sakai
Pluripotent stem cells (PSCs) are one of the promising cell sources for tissue engineering and drug screening. However, mass production of induced pluripotent stem cells (iPSCs) is still developing. Especially, a huge amount of culture medium usage causes expensive cost in the mass production process. In this report, we reduced culture medium usage by extending interval of changing culture medium. In parallel, we also increased glucose concentration and supplied heparan sulfate to avoid depletion of glucose and bFGF respectively...
August 29, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28855541/determination-of-the-physiological-and-pathological-roles-of-e2f3-in-adult-tissues
#7
Ivonne Gamper, Deborah L Burkhart, Megan J Bywater, Daniel Garcia, Catherine H Wilson, Peter A Kreuzaler, Mark J Arends, Yao-Wu Zheng, Alessandra Perfetto, Trevor D Littlewood, Gerard I Evan
While genetically engineered mice have made an enormous contribution towards the elucidation of human disease, it has hitherto not been possible to tune up or down the level of expression of any endogenous gene. Here we describe compound genetically modified mice in which expression of the endogenous E2f3 gene may be either reversibly elevated or repressed in adult animals by oral administration of tetracycline. This technology is, in principle, applicable to any endogenous gene, allowing direct determination of both elevated and reduced gene expression in physiological and pathological processes...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28820389/hippo-pathway-brief-overview-of-its-relevance-in-cancer
#8
A L Zygulska, K Krzemieniecki, P Pierzchalski
The Hippo pathway is the major regulator of organ growth and proliferation. Described initially in Drosophila, it is now recognized as one of the most conserved molecular pathways in all metazoan. Recent studies have revealed the Hippo signalling pathway might contribute to tumorigenesis and cancer development. The core components of the Hippo pathway include the mammalian sterile 20-like kinases (MSTs), large tumour suppressor kinases (LATSs), the adaptor proteins Salvador homologue 1 (SAV1, also called WW45) and Mps One Binder kinase activator proteins...
June 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28781077/embryonic-stem-cell-differentiation-to-functional-arterial-endothelial-cells-through-sequential-activation-of-etv2-and-notch1-signaling-by-hif1%C3%AE
#9
Kit Man Tsang, James S Hyun, Kwong Tai Cheng, Micaela Vargas, Dolly Mehta, Masuko Ushio-Fukai, Li Zou, Kostandin V Pajcini, Jalees Rehman, Asrar B Malik
The generation of functional arterial endothelial cells (aECs) from embryonic stem cells (ESCs) holds great promise for vascular tissue engineering. However, the mechanisms underlying their generation and the potential of aECs in revascularizing ischemic tissue are not fully understood. Here, we observed that hypoxia exposure of mouse ESCs induced an initial phase of HIF1α-mediated upregulation of the transcription factor Etv2, which in turn induced the commitment to the EC fate. However, sustained activation of HIF1α in these EC progenitors thereafter induced NOTCH1 signaling that promoted the transition to aEC fate...
September 12, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28776092/hepatocytic-differentiation-of-ips-cells-on-decellularized-liver-tissue
#10
Mitsuhi Hirata, Tetsuji Yamaoka
Decellularized tissues (DETs) have been attracting great attention as scaffolds for tissue-engineering approaches. Recently, some studies have reported that decellularized liver tissues (DLT) can provide an excellent environment for the hepatocytic differentiation of hepatic stem/progenitor cells that were already committed to the hepatocyte lineage. However, the effects of DLT on the hepatocytic differentiation of induced pluripotent stem cells (iPSs) have not yet been established. Here we studied the hepatocytic differentiation of iPSs on DLT and decellularized heart tissues (DHT) in order to determine the tissue-specific effects of DETs on iPSs differentiation...
August 3, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/28714175/schwann-cells-secrete-extracellular-vesicles-to-promote-and-maintain-the-proliferation-and-multipotency-of-hdpcs
#11
Ziyue Li, Yan Liang, Kuangwu Pan, Hui Li, Mei Yu, Weihua Guo, Guoqing Chen, Weidong Tian
OBJECTIVES: Schwann cells (SCs) are the principal glial cells in peripheral nerve system, involved in neuropathies with great regenerative potential. Dental pulp cells have been reported to maintain neurogenic potential. In contrast, the regulatory role of SCs on human dental pulp cells (hDPCs) development remains undefined. MATERIALS AND METHODS: SC secretion and SC-derived extracellular vesicles (EVs) were collected and used to treat hDPCs; and proliferation and multiple differentiation of hDPCs were detected after EVs treatments...
August 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28711779/hyperactive-foxo1-results-in-lack-of-tip-stalk-identity-and-deficient-microvascular-regeneration-during-kidney-injury
#12
Lan T H Dang, Takahide Aburatani, Graham A Marsh, Bryce G Johnson, Stella Alimperti, Christine J Yoon, Angela Huang, Suzanne Szak, Naoki Nakagawa, Ivan Gomez, Shuyu Ren, Sarah K Read, Chris Sparages, Alfred C Aplin, Roberto F Nicosia, Chris Chen, Giovanni Ligresti, Jeremy S Duffield
Loss of the microvascular (MV) network results in tissue ischemia, loss of tissue function, and is a hallmark of chronic diseases. The incorporation of a functional vascular network with that of the host remains a challenge to utilizing engineered tissues in clinically relevant therapies. We showed that vascular-bed-specific endothelial cells (ECs) exhibit differing angiogenic capacities, with kidney microvascular endothelial cells (MVECs) being the most deficient, and sought to explore the underlying mechanism...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28705193/spatial-regulation-of-monolignol-biosynthesis-and-laccase-genes-control-developmental-and-stress-related-lignin-in-flax
#13
Julien Le Roy, Anne-Sophie Blervacq, Anne Créach, Brigitte Huss, Simon Hawkins, Godfrey Neutelings
BACKGROUND: Bast fibres are characterized by very thick secondary cell walls containing high amounts of cellulose and low lignin contents in contrast to the heavily lignified cell walls typically found in the xylem tissues. To improve the quality of the fiber-based products in the future, a thorough understanding of the main cell wall polymer biosynthetic pathways is required. In this study we have carried out a characterization of the genes involved in lignin biosynthesis in flax along with some of their regulation mechanisms...
July 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28699694/the-epigenomics-of-schizophrenia-in-the-mouse
#14
REVIEW
Behnam Javidfar, Royce Park, Bibi S Kassim, Lucy K Bicks, Schahram Akbarian
Large-scale consortia including the Psychiatric Genomics Consortium, the Common Minds Consortium, BrainSeq and PsychENCODE, and many other studies taken together provide increasingly detailed insights into the genetic and epigenetic risk architectures of schizophrenia (SCZ) and offer vast amounts of molecular information, but with largely unexplored therapeutic potential. Here we discuss how epigenomic studies in human brain could guide animal work to test the impact of disease-associated alterations in chromatin structure and function on cognition and behavior...
September 2017: American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics
https://www.readbyqxmd.com/read/28690010/tailoring-surface-nanoroughness-of-electrospun-scaffolds-for-skeletal-tissue-engineering
#15
Honglin Chen, Xiaobin Huang, Minmin Zhang, Febriyani Damanik, Matthew B Baker, Anne Leferink, Huipin Yuan, Roman Truckenmüller, Clemens van Blitterswijk, Lorenzo Moroni
Electrospun scaffolds provide a promising approach for tissue engineering as they mimic the physical properties of extracellular matrix. Previous studies have demonstrated that electrospun scaffolds with porous features on the surface of single fibers, enhanced cellular attachment and proliferation. Yet, little is known about the effect of such topographical cues on cellular differentiation. Here, we aimed at investigating the influence of surface roughness of electrospun scaffolds on skeletal differentiation of human mesenchymal stromal cells (hMSCs)...
September 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28687929/periostin-promotes-ectopic-osteogenesis-of-ctla4-modified-bone-marrow-mesenchymal-stem-cells
#16
Fei Zhang, Zhigang Rong, Zhengdong Wang, Zehua Zhang, Dong Sun, Shiwu Dong, Jianzhong Xu, Fei Dai
The improved ectopic osteogenesis of cytotoxic T-lymphocyte-associated antigen 4-Ig-modified bone marrow mesenchymal stem cells (MSCs-CTLA4) has been demonstrated but the mechanisms involved remain to be determined. The extracellular matrix (ECM) has recently been reported to play a vital role in bone formation and periostin (POSTN) has been suggested as a key member in constructing the ECM in bone tissue. We found that POSTN expression in the MSCs-CTLA4 group is significantly enhanced compared with that in the MSCs group, not only in tissue-engineered bone (TEB) with femur heterotopic transplantation in vivo but also under the immune activation condition in vitro...
July 8, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28686585/constructing-cellular-niche-properties-by-localized-presentation-of-wnt-proteins-on-synthetic-surfaces
#17
Molly Lowndes, Sergi Junyent, Shukry J Habib
Wnt signaling is crucial during embryonic development and for the maintenance of adult tissues. Depending on the tissue type, the Wnt pathway can promote stem cell self-renewal and/or direct lineage commitment. Wnt proteins are subject to lipid modification, often restricting them to act in a localized manner on responsive cells. Most methods for inducing Wnt signaling in stem cell cultures do not control the spatial presentation of the protein. To recreate the local presentation of Wnt proteins often seen in vivo, we previously developed a method to immobilize the protein onto synthetic surfaces...
July 2017: Nature Protocols
https://www.readbyqxmd.com/read/28677757/hypertrophic-differentiation-of-mesenchymal-stem-cells-is-suppressed-by-xanthotoxin-via-the-p38%C3%A2-mapk-hdac4-pathway
#18
Zhen Cao, Yun Bai, Chuan Liu, Ce Dou, Jianmei Li, Junyu Xiang, Chunrong Zhao, Zhao Xie, Qiang Xiang, Shiwu Dong
Chondrocyte hypertrophy is a physiological process in endochondral ossification. However, the hypertrophic‑like alterations of chondrocytes at the articular surface may result in osteoarthritis (OA). In addition, the generation of fibrocartilage with a decreased biological function in tissue engineered cartilage, has been attributed to chondrocyte hypertrophy. Therefore, suppressing chondrocyte hypertrophy in OA and the associated regeneration of non‑active cartilage is of primary concern. The present study examined the effects of xanthotoxin (XAT), which is classified as a furanocoumarin, on chondrocyte hypertrophic differentiation of mesenchymal stem cells...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28676126/umbilical-cord-derived-mesenchymal-stromal-cells-predictive-obstetric-factors-for-cell-proliferation-and-chondrogenic-differentiation
#19
Léonore Avercenc-Léger, Philippe Guerci, Jean-Marc Virion, Ghislaine Cauchois, Sébastien Hupont, Rachid Rahouadj, Jacques Magdalou, Jean-François Stoltz, Danièle Bensoussan, Céline Huselstein, Loïc Reppel
BACKGROUND: The umbilical cord is becoming a notable alternative to bone marrow (BM) as a source of mesenchymal stromal cells (MSC). Although age-dependent variations in BM-MSC are well described, less data are available for MSC isolated from Wharton's jelly (WJ-MSC). We initiated a study to identify whether obstetric factors influenced MSC properties. We aimed to evaluate the correlation between a large number of obstetric factors collected during pregnancy and until peripartum (related to the mother, the labor and delivery, and the newborn) with WJ-MSC proliferation and chondrogenic differentiation parameters...
July 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28659188/comparative-transcriptome-analysis-of-shoot-and-root-tissue-of-bacopa-monnieri-identifies-potential-genes-related-to-triterpenoid-saponin-biosynthesis
#20
Gajendra Singh Jeena, Shahnoor Fatima, Pragya Tripathi, Swati Upadhyay, Rakesh Kumar Shukla
BACKGROUND: Bacopa monnieri commonly known as Brahmi is utilized in Ayurveda to improve memory and many other human health benefits. Bacosides enriched standardized extract of Bacopa monnieri is being marketed as a memory enhancing agent. In spite of its well known pharmacological properties it is not much studied in terms of transcripts involved in biosynthetic pathway and its regulation that controls the secondary metabolic pathway in this plant. The aim of this study was to identify the potential transcripts and provide a framework of identified transcripts involved in bacosides production through transcriptome assembly...
June 28, 2017: BMC Genomics
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