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Transcription factor tissue engineering

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https://www.readbyqxmd.com/read/29734737/inhibition-of-v-rel-induced-oncogenesis-through-microrna-targeting
#1
Yongxiu Yao, Yaoyao Zhang, Na Tang, Miriam Pedrera, Zhiqiang Shen, Venugopal Nair
Several studies have shown that microRNA-targeting is an effective strategy for the selective control of tissue-tropism and pathogenesis of both DNA and RNA viruses. However, the exploitation of microRNA-targeting for the inhibition of transformation by oncogenic viruses has not been studied. The v- rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel /NF-κB family of transcription factors capable of transforming primary chicken spleen and bone marrow cells...
May 5, 2018: Viruses
https://www.readbyqxmd.com/read/29726060/differential-regulation-of-human-bone-marrow-mesenchymal-stromal-cell-chondrogenesis-by-hypoxia-inducible-factor-1%C3%AE-hydroxylase-inhibitors
#2
Dheraj K Taheem, Daniel A Foyt, Sandra Loaiza, Silvia A Ferreira, Dusko Ilic, Holger W Auner, Agamemnon E Grigoriadis, Gavin Jell, Eileen Gentleman
The transcriptional profile induced by hypoxia plays important roles in the chondrogenic differentiation of marrow stromal/stem cells (MSC) and is mediated by the Hypoxia Inducible Factor complex. However, various compounds can also stabilise HIF's oxygen-responsive element, HIF-1α, at normoxia and mimic many hypoxia-induced cellular responses. Such compounds may prove efficacious in cartilage tissue engineering, where microenvironmental cues may mediate functional tissue formation. Here, we investigated three HIF stabilising compounds, which each have distinct mechanisms of action, to understand how they differentially influenced the chondrogenesis of human bone marrow-derived MSC (hBM-MSC) in vitro...
May 3, 2018: Stem Cells
https://www.readbyqxmd.com/read/29725268/mesoporous-bioactive-glass-coated-3d-printed-borosilicate-bioactive-glass-scaffolds-for-improving-repair-of-bone-defects
#3
Xin Qi, Hui Wang, Yadong Zhang, Libin Pang, Wei Xiao, Weitao Jia, Shichang Zhao, Deping Wang, Wenhai Huang, Qiugen Wang
Background: In the field of tissue engineering, there is currently increasing interest in new biomedical materials with high osteogenic ability and comparable mechanical function to repair bone defects. Three-dimensional (3-D) bioactive borosilicate glass (BG) scaffolds exhibit uniform interconnected macro-pores, high porosity and high compressive strength. In this study, we fabricated 3-D BG scaffolds by the 3D printing technique, then coated the surface of the 3-D BG scaffolds with mesoporous bioactive glass (MBG) (BG-MBG scaffold)...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29713381/switchgrass-panicum-virgatum-l-promoters-for-green-tissue-specific-expression-of-the-myb4-transcription-factor-for-reduced-recalcitrance-transgenic-switchgrass
#4
Wusheng Liu, Mitra Mazarei, Rongjian Ye, Yanhui Peng, Yuanhua Shao, Holly L Baxter, Robert W Sykes, Geoffrey B Turner, Mark F Davis, Zeng-Yu Wang, Richard A Dixon, C Neal Stewart
Background: Genetic engineering of switchgrass ( Panicum virgatum L.) for reduced cell wall recalcitrance and improved biofuel production has been a long pursued goal. Up to now, constitutive promoters have been used to direct the expression of cell wall biosynthesis genes toward attaining that goal. While generally sufficient to gauge a transgene's effects in the heterologous host, constitutive overexpression often leads to undesirable plant phenotypic effects. Green tissue-specific promoters from switchgrass are potentially valuable to directly alter cell wall traits exclusively in harvestable aboveground biomass while not changing root phenotypes...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29710848/biofunctionalized-lysophosphatidic-acid-silk-fibroin-film-for-cornea-endothelial-cell-regeneration
#5
Joo Hee Choi, Hayan Jeon, Jeong Eun Song, Joaquim Miguel Oliveira, Rui Luis Reis, Gilson Khang
Cornea endothelial cells (CEnCs) tissue engineering is a great challenge to repair diseased or damaged CEnCs and require an appropriate biomaterial to support cell proliferation and differentiation. Biomaterials for CEnCs tissue engineering require biocompatibility, tunable biodegradability, transparency, and suitable mechanical properties. Silk fibroin-based film (SF) is known to meet these factors, but construction of functionalized graft for bioengineering of cornea is still a challenge. Herein, lysophosphatidic acid (LPA) is used to maintain and increase the specific function of CEnCs...
April 30, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29693145/icariin-promotes-stable-chondrogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-in-self%C3%A2-assembling-peptide-nanofiber-hydrogel-scaffolds
#6
Zhicong Wang, Kaihua Li, Huijun Sun, Ji Wang, Zhuodong Fu, Mozhen Liu
Icariin, a traditional Chinese medicine, has previously been demonstrated to promote chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) in traditional 2D cell culture. The present study investigated whether icariin has the potential to promote stable chondrogenic differentiation of BMSCs without hypertrophy in a 3D microenvironment. BMSCs were cultivated in a self‑assembling peptide nanofiber hydrogel scaffold in chondrogenic medium for 3 weeks. Icariin was added to the medium throughout the culture period at concentrations of 1x10‑6 M...
April 23, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29689281/fabrication-of-engineered-tubular-tissue-for-small-blood-vessels-via-three-dimensional-cellular-assembly-and-organization-ex-vivo
#7
Taisuke Masuda, Mitsuhiro Ukiki, Yuka Yamagishi, Michiya Matsusaki, Mitsuru Akashi, Utako Yokoyama, Fumihito Arai
Although there is a great need for suitable vascular replacements in clinical practice, much progress needs to be made toward the development of a fully functional tissue-engineered construct. We propose a fabrication method of engineered tubular tissue for small blood vessels via a layer-by-layer cellular assembly technique using mouse smooth muscle cells, the construction of a poly-(L-lactide-co-ε-caprolactone) (PLCL) scaffold, and integration in a microfluidic perfusion culture system. The cylindrical PLCL scaffold is incised, expanded, and its surface is laminated with the cell layers...
April 21, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29682901/engineering-storage-capacity-for-volatile-sesquiterpenes-in-nicotiana-benthamiana-leaves
#8
Thierry L Delatte, Giulia Scaiola, Jamil Molenaar, Katyuce de Sousa Farias, Leticia Alves Gomes Albertti, Jacqueline Busscher, Francel Verstappen, Carlos Carollo, Harro Bouwmeester, Jules Beekwilder
Plants store volatile compounds in specialized organs. The properties of these storage organs prevent precarious evaporation and protect neighbouring tissues from cytotoxicity. Metabolic engineering of plants is often done in tissues such as leaf mesophyll cells, which are abundant and easily accessible by engineering tools. However, these tissues are not suitable for the storage of volatile and hydrophobic compound such as sesquiterpenes and engineered volatiles are often lost into the headspace. In the present study we show that the seeds of Arabidopsis thaliana, which naturally contain lipid bodies, accumulate sesquiterpenes upon engineered expression...
April 23, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29545723/coordinated-transcription-of-anril-and-p16-genes-is-silenced-by-p16-dna-methylation
#9
Ying Gan, Wanru Ma, Xiuhong Wang, Juanli Qiao, Baozhen Zhang, Chenghua Cui, Zhaojun Liu, Dajun Deng
Objective: To investigate the relationship between the transcription of ANRIL , P15 , P14 and P16 at the same locus and the regulation mechanism of ANRIL . Methods: Publicly available database of Cancer Cell Line Encyclopedia (CCLE) was used in bioinformatic analyses. Methylation of CpG islands was detected by denaturing high performance liquid chromatography (DHPLC). Gene transcript levels were determined using quantitative real-time polymerase chain reaction (qRT-PCR) assays...
February 2018: Chinese Journal of Cancer Research, Chung-kuo Yen Cheng Yen Chiu
https://www.readbyqxmd.com/read/29533181/nurd-and-caf-1-mediated-silencing-of-the-d4z4-array-is-modulated-by-dux4-induced-mbd3l-proteins
#10
Amy E Campbell, Sean C Shadle, Sujatha Jagannathan, Jong-Won Lim, Rebecca Resnick, Rabi Tawil, Silvère M van der Maarel, Stephen J Tapscott
The DUX4 transcription factor is encoded by a retrogene embedded in each unit of the D4Z4 macrosatellite repeat. DUX4 is normally expressed in the cleavage-stage embryo, whereas chromatin repression prevents DUX4 expression in most somatic tissues. Failure of this repression causes facioscapulohumeral muscular dystrophy (FSHD) due to mis-expression of DUX4 in skeletal muscle. In this study, we used CRISPR/Cas9 engineered chromatin immunoprecipitation (enChIP) locus-specific proteomics to characterize D4Z4-associated proteins...
March 13, 2018: ELife
https://www.readbyqxmd.com/read/29528434/a-bhlh-transcription-factor-regulates-bisbibenzyl-biosynthesis-in-the-liverwort-plagiochasma-appendiculatum
#11
Yi-Feng Wu, Yu Zhao, Xin-Yan Liu, Shuai Gao, Ai-Xia Cheng, Hong-Xiang Lou
Liverworts, a section of the bryophyte plants which pioneered the colonization of terrestrial habitats, produce cyclic bisbibenzyls as a secondary metabolite. These compounds are generated via the phenylpropanoid pathway, similar with flavonoid biosynthesis for which bHLH transcription factors have been identified as one of the improtant regulators in higher plants. Here, a bHLH homolog (PabHLH) was isolated from the liverwort species Plagiochasma appendiculatum and its contribution to bisbibenzyl biosynthesis was explored...
March 8, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29527327/dna-methylation-profile-is-associated-with-the-osteogenic-potential-of-three-distinct-human-odontogenic-stem-cells
#12
Tingting Ai, Jieni Zhang, Xuedong Wang, Xiaowen Zheng, Xueyan Qin, Qian Zhang, Weiran Li, Wei Hu, Jiuxiang Lin, Feng Chen
Among the various sources of human autologous stem cells, stem cells isolated from dental tissues exhibit excellent properties in tissue engineering and regenerative medicine. However, the distinct potential of these odontogenic cell lines remains unclear. In this study, we analyzed DNA methylation patterns to determine whether specific differences existed among three different odontogenic cell types. Using the HumanMethylation450 Beadchip, the whole genomes of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and dental follicle progenitor cells (DFPCs) were compared...
2018: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29523820/zfp226-is-a-novel-artificial-transcription-factor-for-selective-activation-of-tumor-suppressor-kibra
#13
Katrin Schelleckes, Boris Schmitz, Malte Lenders, Mirja Mewes, Stefan-Martin Brand, Eva Brand
KIBRA has been suggested as a key regulator of the hippo pathway, regulating organ size, cell contact inhibition as well as tissue regeneration and tumorigenesis. Recently, alterations of KIBRA expression caused by promotor methylation have been reported for several types of cancer. Our current study aimed to design an artificial transcription factor capable of re-activating expression of the tumor suppressor KIBRA and the hippo pathway. We engineered a new gene named 'ZFP226' encoding for a ~23 kDa fusion protein...
March 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29523191/lrrc15-promotes-osteogenic-differentiation-of-mesenchymal-stem-cells-by-modulating-p65-cytoplasmic-nuclear-translocation
#14
Yuejun Wang, Yunsong Liu, Min Zhang, Longwei Lv, Xiao Zhang, Ping Zhang, Yongsheng Zhou
BACKGROUND: Mesenchymal stem cells (MSCs) are a reliable resource for bone regeneration and tissue engineering, but the molecular mechanisms of differentiation remain unclear. The tumor antigen 15-leucine-rich repeat containing membrane protein (LRRC15) is a transmembrane protein demonstrated to play important roles in cancer. However, little is known about its role in osteogenesis. This study was to evaluate the functions of LRRC15 in osteogenic differentiation of MSCs. METHODS: Osteogenic-induction treatment and the ovariectomized (OVX) model were performed to investigate the potential relationship between LRRC15 and MSC osteogenesis...
March 9, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29508544/generation-and-characterization-of-lhx3-gfp-reporter-knockin-and-lhx3-loxp-conditional-knockout-mice
#15
Mei Xu, Xiaoling Xie, Xuhui Dong, Guoqing Liang, Lin Gan
LHX3, a LIM-homeodomain transcription factor, is broadly expressed in the developing pituitary, spinal cord, medulla, retina and inner ear, and plays essential roles during embryonic development. Mice with homozygous Lhx3 null mutation exhibit failure in the formation of pituitary gland and die perinatally. To facilitate the functional study of Lhx3 in mice, we engineered and characterized two novel Lhx3 mouse strains: Lhx3GFP reporter knock-in and Lhx3loxP conditional knockout mice. Coimmunolabeling of LHX3 and GFP shows that the expression pattern of the knock-in GFP reporter recapitulates that of endogenous LHX3 in cochlea, vestibule, retina, and spinal cord...
April 2018: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/29502489/chitosan-oligosaccharide-promotes-osteoclast-formation-by-stimulating-the-activation-of-mapk-and-akt-signaling-pathways
#16
Bing-Li Bai, Zhong-Jie Xie, She-Ji Weng, Zong-Yi Wu, Hang Li, Zhou-Shan Tao, Viraj Boodhun, De-Yi Yan, Zi-Jian Shen, Jia-Hao Tang, Lei Yang
Chitosan Oligosaccharide (COS) has been widely used for the systemic treatment of clinical diseases such as bone tissue engineering. However, its influence on osteoclast formation, which plays a critical role in bone homeostasis, has never been investigated. The aim of this study was to investigate the effect of chitosan oligosaccharide on differentiation of osteoclast. Using cell counting kit-8, tartrate-resistant acid phosphatase staining, reverse transcription‑quantitative polymerase chain reaction assay and western blot analysis, we demonstrated that chitosan oligosaccharide cannot inhibit RANKL-induced osteoclast precursor proliferation but does promote osteoclast differentiation by stimulating the activation of p38/mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK)/MAPK, extracellular signal-regulated kinase (ERK)/MAPK and protein kinase B (AKT) without affecting nuclear factor kappaB (NF-kB) signaling pathways...
July 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29490603/harnessing-a-novel-inhibitory-role-of-mir-16-in-osteogenesis-by-human-mesenchymal-stem-cells-for-advanced-scaffold-based-bone-tissue-engineering
#17
Irene Mencía Castaño, Caroline M Curtin, Garry P Duffy, Fergal J O'Brien
MicroRNA (miRNA) therapeutics is increasingly being developed to either target bone-related diseases such as osteoporosis and osteoarthritis or as the basis for novel bone tissue engineering strategies. A number of miRNAs have been reported as potential osteo-therapeutics but no consensus has yet been established on the optimal target. miR-16 has been studied extensively in nonosteogenic functions and used as functionality reporter target in the development of nonviral miRNA delivery platforms. This study hypothesized that miR-16 may also play an inhibitory role in osteogenesis due to its ability to directly target Smad5 and AcvR2a...
April 10, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29490590/effect-of-brain-derived-neurotrophic-factor-on-the-neurogenesis-and-osteogenesis-in-bone-engineering
#18
Qing Liu, Lei Lei, Tao Yu, Ting Jiang, Yunqing Kang
During bone growth, the lack of a neuralized vascular network in the regenerating area can affect subsequent bone quality. This study aimed to investigate if brain-derived neurotrophic factor (BDNF) could promote neurogenesis and osteogenesis in human bone mesenchymal stem cells (hBMSCs) to improve bone formation during tissue engineering. Initially, a safe and effective BDNF concentration that facilitated hBMSC proliferation in vitro was determined. Subsequently, examination of mineralized nodule formation and evaluation of alkaline phosphatase (ALP) activity and ALP gene expression revealed that the most effective concentration of BDNF to elicit a response in hBMSCs was 100 ng/mL...
April 10, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29486602/generation-of-insulin-producing-cells-from-human-induced-pluripotent-stem-cells-on-plla-pva-nanofiber-scaffold
#19
Seyed Ehsan Enderami, Mousa Kehtari, Mohammad Foad Abazari, Pegah Ghoraeian, Maryam Nouri Aleagha, Fatemeh Soleimanifar, Masoud Soleimani, Yousef Mortazavi, Samad Nadri, Hossein Mostafavi, Hassan Askari
Pancreatic tissue engineering as a therapeutic option for restoring and maintenance of damaged pancreas function has a special focus to using synthetic Scaffolds. This study was designed to evaluate pancreatic differentiation of human induced pluripotent stem cells (hiPSCs) on poly-L-lactic acid and polyvinyl alcohol (PLLA/PVA) scaffolds as 3 D matrix. During differentiation process, morphology of cells gradually changed and iPSCs derived insulin producing cells (iPSCs-IPCs) formed spherical shaped cell aggregation that was the typical shape of islets of pancreas...
February 27, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29467198/endothelial-tfeb-transcription-factor-eb-positively-regulates-postischemic-angiogenesis
#20
Yanbo Fan, Haocheng Lu, Wenying Liang, Minerva T Garcia-Barrio, Yanhong Guo, Ji Zhang, Tianqing Zhu, Yibai Hao, Jifeng Zhang, Y Eugene Chen
RATIONALE: Postischemic angiogenesis is critical to limit the ischemic tissue damage and improve the blood flow recovery. The regulation and the underlying molecular mechanisms of postischemic angiogenesis are not fully unraveled. TFEB (transcription factor EB) is emerging as a master gene for autophagy and lysosome biogenesis. However, the role of TFEB in vascular disease is less understood. OBJECTIVE: We aimed to determine the role of endothelial TFEB in postischemic angiogenesis and its underlying molecular mechanism...
March 30, 2018: Circulation Research
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