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Transcription factor tissue engineering

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https://www.readbyqxmd.com/read/29339095/patterning-the-gastrointestinal-epithelium-to-confer-regional-specific-functions
#1
REVIEW
Cayla A Thompson, Ann DeLaForest, Michele A Battle
The gastrointestinal (GI) tract, in simplest terms, can be described as an epithelial-lined muscular tube extending along the cephalocaudal axis from the oral cavity to the anus. Although the general architecture of the GI tract organs is conserved from end to end, the presence of different epithelial tissue structures and unique epithelial cell types within each organ enables each to perform the distinct digestive functions required for efficient nutrient assimilation. Spatiotemporal regulation of signaling pathways and downstream transcription factors controls GI epithelial morphogenesis during development to confer essential regional-specific epithelial structures and functions...
January 12, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29330205/clinical-utility-of-a-stat3-regulated-microrna-200-family-signature-with-prognostic-potential-in-early-gastric-cancer
#2
Liang Yu, Di Wu, Hugh Gao, Jesse Balic, Anna Tsykin, Tae-Su Han, You Dong Liu, Catherine Lydia Kennedy, Ji-Kun Li, Jie-Qi Mao, Patrick Tan, Masanobu Oshima, Gregory J Goodall, Brendan J Jenkins
PURPOSE: The majority of gastric cancer (GC) patients are diagnosed with late stage disease, for which distinct molecular subtypes have been identified that are potentially amenable to targeted therapies. However, there exists no molecular classification system with prognostic power for early stage GC (EGC) because the molecular events promoting GC initiation remain ill-defined. EXPERIMENTAL DESIGN: miRNA microarrays were performed on gastric tissue from the gp130F/F preclinical EGC mouse model, prior to tumor initiation...
January 12, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29316809/extracorporeal-shock-waves-trigger-tenogenic-differentiation-of-human-adipose-derived-stem-cells
#3
Letizia Rinella, Francesca Marano, Laura Paletto, Marco Fraccalvieri, Laura Annaratone, Isabella Castellano, Nicoletta Fortunati, Alessandro Bargoni, Laura Berta, Roberto Frairia, Maria Graziella Catalano
PURPOSES: Incomplete tendon healing impairs the outcome of tendon ruptures and tendinopathies. Human Adipose-derived Stem Cells (hASCs) are promising for tissue engineering applications. Extracorporeal Shock Waves (ESW) are a leading choice for the treatment of several tendinopathies. In this study, we investigated the effects of ESW treatment and tenogenic medium on the differentiation of hASCs into tenoblast-like cells. MATERIALS AND METHODS: hASCs were treated with ESW generated by a piezoelectric device and tenogenic medium...
January 9, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29300346/the-osteogenic-differentiation-effect-of-the-fn-type-10-peptide-amphiphile-on-pcl-fiber
#4
Ye-Rang Yun, Hae-Won Kim, Jun-Hyeog Jang
The fibronectin type 10-peptide amphiphile (FNIII10-PA) was previously genetically engineered and showed osteogenic differentiation activity on rat bone marrow stem cells (rBMSCs). In this study, we investigated whether FNIII10-PA demonstrated cellular activity on polycaprolactone (PCL) fibers. FNIII10-PA significantly increased protein production and cell adhesion activity on PCL fibers in a dose-dependent manner. In cell proliferation results, there was no effect on cell proliferation activity by FNIII10-PA; however, FNIII10-PA induced the osteogenic differentiation of MC3T3-E1 cells via upregulation of bone sialoprotein (BSP), collagen type I (Col I), osteocalcin (OC), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) mitochondrial RNA (mRNA) levels; it did not increase the alkaline phosphatase (ALP) mRNA level...
January 4, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29289734/conductive-nanofibrous-composite-scaffolds-based-on-in-situ-formed-polyaniline-nanoparticle-and-polylactide-for-bone-regeneration
#5
Jing Chen, Meng Yu, Baolin Guo, Peter X Ma, Zhanhai Yin
Conducting polymers and biodegradable polylactide (PLA) scaffolds are both promising biomaterials applied in bone tissue engineering. It is necessary to develop a composite scaffold combining their properties of osteogenic differentiation promotion and three-dimension matrix. To conquer the problem of poor processability of conductive polymers, we use a novel in-situ polymerization/thermal induced phase separation (TIPS) method to fabricate conductive nanofibrous PLA scaffolds with well-distributed polyaniline (PANI) nano-structures...
December 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29240236/evaluation-of-osteoblast-differentiation-and-function-when-cultured-on-mesoporous-bioactive-glass-adsorbed-with-testosterone
#6
Kai Gao, Xiaoyan Wang, Qianqian Liu, Wei Chen, Gan Wang, Dongyi Zhang, Long Liu
Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure and large pore volume. Here we reported that the proliferation, differentiation and mineralization were promoted in MC3T3-E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3-E1 cells cultured on MBG/T...
December 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29222839/decellularized-human-liver-extracellular-matrix-hdlm-mediated-hepatic-differentiation-of-human-induced-pluripotent-stem-cells-hipsc
#7
Maria Jaramillo, Heidi Yeh, Martin L Yarmush, Basak E Uygun
Liver tissue engineering has emerged as a promising approach in organ transplantation, but has been hampered by the lack of a reliable and readily available cell source. Induced pluripotent cells (hiPSC) have been highlighted a desirable source, due to their differentiation potential, ability to self-renew and the possibility of making patient specific cells. We developed a decellularization protocol that efficiently removes cellular material, while retaining extracellular matrix components. Subsequently, hiPSC were differentiated on the decellularized human liver matrix (hDLM) scaffolds using an established hepatic differentiation protocol...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29213288/microrna-214-suppresses-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-atf4
#8
Siqi Yao, Wei Zhao, Qianmin Ou, Lanchen Liang, Xuefeng Lin, Yan Wang
Periodontitis is the main cause of adult tooth loss. Stem cell-based tissue engineering has become a promising therapy for periodontitis treatment. To date, human periodontal ligament stem cells (hPDLSCs) have been shown to be a favorable source for tissue engineering, but modulatory mechanisms of hPDLSCs remain unclear. Approximately 60% of mammalian genes are the targets of over 2000 miRNAs in multiple human cell types, and miRNAs are able to influence various biological processes in the human body, including bone formation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29174331/constitutively-active-smad2-3-are-broad-scope-potentiators-of-transcription-factor-mediated-cellular-reprogramming
#9
Tyson Ruetz, Ulrich Pfisterer, Bruno Di Stefano, James Ashmore, Meryam Beniazza, Tian V Tian, Daniel F Kaemena, Luca Tosti, Wenfang Tan, Jonathan R Manning, Eleni Chantzoura, Daniella Rylander Ottosson, Samuel Collombet, Anna Johnsson, Erez Cohen, Kosuke Yusa, Sten Linnarsson, Thomas Graf, Malin Parmar, Keisuke Kaji
Reprogramming of cellular identity using exogenous expression of transcription factors (TFs) is a powerful and exciting tool for tissue engineering, disease modeling, and regenerative medicine. However, generation of desired cell types using this approach is often plagued by inefficiency, slow conversion, and an inability to produce mature functional cells. Here, we show that expression of constitutively active SMAD2/3 significantly improves the efficiency of induced pluripotent stem cell (iPSC) generation by the Yamanaka factors...
November 13, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/29172997/identification-of-anxa2-annexin-a2-as-a-specific-bleomycin-target-to-induce-pulmonary-fibrosis-by-impeding-tfeb-mediated-autophagic-flux
#10
Kui Wang, Tao Zhang, Yunlong Lei, Xuefeng Li, Jingwen Jiang, Jiang Lan, Yuan Liu, Haining Chen, Wei Gao, Na Xie, Qiang Chen, Xiaofeng Zhu, Xiang Liu, Ke Xie, Yong Peng, Edouard C Nice, Min Wu, Canhua Huang, Yuquan Wei
Bleomycin is a clinically potent anticancer drug used for the treatment of germ-cell tumors, lymphomas and squamous-cell carcinomas. Unfortunately, the therapeutic efficacy of bleomycin is severely hampered by the development of pulmonary fibrosis. However, the mechanisms underlying bleomycin-induced pulmonary fibrosis, particularly the molecular target of bleomycin, remains unknown. Here, using a chemical proteomics approach, we identify ANXA2 (annexin A2) as a direct binding target of bleomycin. The interaction of bleomycin with ANXA2 was corroborated both in vitro and in vivo...
November 26, 2017: Autophagy
https://www.readbyqxmd.com/read/29169271/differentiation-of-mesenchymal-stem-cells-into-neuron-like-cells-using-composite-3d-scaffold-combined-with-valproic-acid-induction
#11
Sadegh Ghorbani, Taki Tiraihi, Masoud Soleimani
The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines neural stem, scaffolds, and growth factors may improve the chance of restoration of damaged neural tissues. A favorable niche for neural regeneration would be both fibrous and electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wet-electrospun 3D scaffolds composed of poly lactic acid coated with natural polymers including alginate and gelatin, followed by a multi-wall carbon nanotube coating...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29159895/proliferation-and-differentiation-of-mesenchymal-stem-cells-on-scaffolds-containing-chitosan-calcium-polyphosphate-and-pigeonite-for-bone-tissue-engineering
#12
S Dhivya, A Keshav Narayan, R Logith Kumar, S Viji Chandran, M Vairamani, N Selvamurugan
OBJECTIVES: Treatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP) and Pg particles for bone formation in vitro and in vivo...
November 21, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29155979/epigenetic-editing-of-the-dlg4-psd95-gene-improves-cognition-in-aged-and-alzheimer-s-disease-mice
#13
Fernando J Bustos, Estibaliz Ampuero, Nur Jury, Rodrigo Aguilar, Fahimeh Falahi, Jorge Toledo, Juan Ahumada, Jaclyn Lata, Paula Cubillos, Berta Henríquez, Miguel V Guerra, Jimmy Stehberg, Rachael L Neve, Nibaldo C Inestrosa, Ursula Wyneken, Marco Fuenzalida, Steffen Härtel, Miguel Sena-Esteves, Lorena Varela-Nallar, Marianne G Rots, Martin Montecino, Brigitte van Zundert
The Dlg4 gene encodes for post-synaptic density protein 95 (PSD95), a major synaptic protein that clusters glutamate receptors and is critical for plasticity. PSD95 levels are diminished in ageing and neurodegenerative disorders, including Alzheimer's disease and Huntington's disease. The epigenetic mechanisms that (dys)regulate transcription of Dlg4/PSD95, or other plasticity genes, are largely unknown, limiting the development of targeted epigenome therapy. We analysed the Dlg4/PSD95 epigenetic landscape in hippocampal tissue and designed a Dlg4/PSD95 gene-targeting strategy: a Dlg4/PSD95 zinc finger DNA-binding domain was engineered and fused to effector domains to either repress (G9a, Suvdel76, SKD) or activate (VP64) transcription, generating artificial transcription factors or epigenetic editors (methylating H3K9)...
November 16, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29152381/selective-deletion-of-the-soluble-colony-stimulating-factor-1-isoform-in-vivo-prevents-estrogen-deficiency-bone-loss-in-mice
#14
Gang-Qing Yao, Nancy Troiano, Christine A Simpson, Karl L Insogna
Neutralizing CSF1 in vivo completely prevents ovariectomy (OVX)-induced bone loss in mice. There are two isoforms of CSF1, soluble (sCSF1), and membrane-bound (mCSF1), but their individual biological functions are unclear. It had been previously reported that mCSF1 knockout (K/O) and wild type (Wt) female mice experience the same degree of bone loss following OVX. In Wt mice the expression of sCSF1 was elevated fourfold in skeletal tissue following OVX while expression of mCSF1 was unchanged. To examine the role of sCSF1 in OVX-induced bone loss, mice were engineered in which sCSF1 was not expressed but expression of mCSF1 was unaffected (sCSF1 K/O)...
2017: Bone Research
https://www.readbyqxmd.com/read/29126771/bmp-2-plasmid-dna-loaded-chitosan-films-a-new-strategy-for-bone-engineering
#15
Juan Li, Jun Lin, Wenke Yu, Xiaojia Song, Qiaoling Hu, Jing-Hong Xu, Huiming Wang, Christian Mehl
OBJECTIVES: Bone defects are common in every area of medicine and remain a clinical challenge. Tissue engineering has led to promising new strategies in accelerating bone repair. Bone morphogenetic proteins (BMPs) play crucial roles in bone regeneration, but are required in supra-physiological doses, which are expensive and produce severe side effects. METHODS: To address these issues, we prepared BMP-2 plasmid DNA-loaded chitosan films, and examined their effects on mouse osteoblast-like MC3T3-E1 cell morphology, proliferation, and runt-related transcription factor 2 (RUNX2) expression...
October 14, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29119879/magnesium-and-zinc-borate-enhance-osteoblastic-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-in-vitro
#16
Yao-Jen Liu, Wen-Ta Su, Po-Hung Chen
Various biocompatible and biodegradable scaffolds blended with biochemical signal molecules with adequate osteoinductive and osteoconductive properties have attracted significant interest in hard tissue engineering regeneration. We evaluated the distinct effects of magnesium borate, zinc borate, and boric acid blended into chitosan scaffold for osteogenic differentiation of stem cells from exfoliated deciduous teeth. Stem cells from exfoliated deciduous teeth cells are a potential source of functional osteoblasts for applications in bone tissue engineering, but the efficiency of osteoblastic differentiation is low, thereby significantly limiting their clinical applications...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29117231/human-pancreatic-islet-derived-extracellular-vesicles-modulate-insulin-expression-in-3d-differentiating-ipsc-clusters
#17
Diana Ribeiro, Eva-Marie Andersson, Nikki Heath, Anette Persson-Kry, Richard Collins, Ryan Hicks, Niek Dekker, Anna Forslöw
It has been suggested that extracellular vesicles (EVs) can mediate crosstalk between hormones and metabolites within pancreatic tissue. However, the possible effect of pancreatic EVs on stem cell differentiation into pancreatic lineages remains unknown. Herein, human islet-derived EVs (h-Islet-EVs) were isolated, characterized and subsequently added to human induced pluripotent stem cell (iPSC) clusters during pancreatic differentiation. The h-islet-EVs had a mean size of 117±7 nm and showed positive expression of CD63 and CD81 EV markers as measured by ELISA...
2017: PloS One
https://www.readbyqxmd.com/read/29115387/generation-of-human-induced-pluripotent-stem-cells-from-burn-patient-derived-skin-fibroblasts-using-a-non-integrative-method
#18
Shangfeng Fu, Jianwu Ding, Dewu Liu, Heping Huang, Min Li, Yang Liu, Longxiang Tu, Deming Liu
Patient specific induced pluripotent stem cells (iPSCs) have been recognized as a possible source of cells for skin tissue engineering. They have the potential to greatly benefit patients with large areas of burned skin or skin defects. However, the integration virus-based reprogramming method is associated with a high risk of genetic mutation and mouse embryonic fibroblast feeder-cells may be a pollutant. In the present study, human skin fibroblasts (HSFs) were successfully harvested from patients with burns and patient-specific iPSCs were generated using a non-integration method with a feeder-free approach...
October 23, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29089527/cross-platform-analysis-of-transcriptomic-data-identifies-ageing-has-distinct-and-opposite-effects-on-tendon-in-males-and-females
#19
Louise I Pease, Peter D Clegg, Carole J Proctor, Daryl J Shanley, Simon J Cockell, Mandy J Peffers
The development of tendinopathy is influenced by a variety of factors including age, gender, sex hormones and diabetes status. Cross platform comparative analysis of transcriptomic data elucidated the connections between these entities in the context of ageing. Tissue-engineered tendons differentiated from bone marrow derived mesenchymal stem cells from young (20-24 years) and old (54-70 years) donors were assayed using ribonucleic acid sequencing (RNA-seq). Extension of the experiment to microarray and RNA-seq data from tendon identified gender specific gene expression changes highlighting disparity with existing literature and published pathways...
October 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29079356/parkin-overexpression-in-human-mesenchymal-stromal-cells-from-wharton-s-jelly-suppresses-6-hydroxydopamine-induced-apoptosis-potential-therapeutic-strategy-in-parkinson-s-disease
#20
A R Bonilla-Porras, A Arevalo-Arbelaez, J F Alzate-Restrepo, C Velez-Pardo, M Jimenez-Del-Rio
BACKGROUND AIMS: Stem cell transplantation is an excellent option for regenerative or replacement therapy. However, deleterious microenvironmental and endogenous factors (e.g., oxidative stress) compromise ongoing graft survival and longevity. Therefore, (transient or stable) genetically modified cells may be reasonably thought to resist oxidative stress-induced damage. Genetic engineering of mesenchymal stromal cells (MSCs) obtained from Wharton's jelly tissue may offer some therapeutic potential...
October 24, 2017: Cytotherapy
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