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Cytokine dynamics

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https://www.readbyqxmd.com/read/29326990/a-fluorescent-microbead-based-microfluidic-immunoassay-chip-for-immune-cell-cytokine-secretion-quantification
#1
Xin Cui, Ya Liu, Dinglong Hu, Weiyi Qian, Chung Tin, Dong Sun, Weiqiang Chen, Raymond H W Lam
Quantitative and dynamic analyses of immune cell secretory cytokines are essential for precise determination and characterization of the "immune phenotype" of patients for clinical diagnosis and treatment of immune-related diseases. Although multiple methods including the enzyme-linked immunosorbent assay (ELISA) have been applied for cytokine detection, such measurements remain very challenging in real-time, high-throughput, and high-sensitivity immune cell analysis. In this paper, we report a highly integrated microfluidic device that allows for on-chip isolation, culture, and stimulation, as well as sensitive and dynamic cytokine profiling of immune cells...
January 12, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29324327/elucidating-the-interfaces-involved-in-card-card-interactions-mediated-by-nlrp1-and-caspase-1-using-molecular-dynamics-simulation
#2
Jitendra Maharana
Inflammasomes are the multi-protein caspase-activating complexes mainly assembled by the sensor proteins (NLRs/ALRs), adaptor molecule (ASC) and effector molecule pro-caspase-1 for the production and release of proinflammatory cytokines, IL-1β and IL-18. NLRP1 is the first NLR known to assemble the multi-protein complex. Unlike NLRP3, NLRP1 has an additional effector binding domain (CARD) at the carboxyl-terminal, which is reported to interact with pro-caspase-1 (precluding the recruitment of ASC) for the transmission of danger signals...
December 24, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29324306/mechanically-resilient-injectable-scaffolds-for-intramuscular-stem-cell-delivery-and-cytokine-release
#3
Stuart A Young, Stephen E Sherman, Tyler T Cooper, Cody Brown, Fraz Anjum, David A Hess, Lauren E Flynn, Brian G Amsden
A promising strategy for treating peripheral ischemia involves the delivery of stem cells to promote angiogenesis through paracrine signaling. Treatment success depends on cell localization, retention, and survival within the mechanically dynamic intramuscular (IM) environment. Herein we describe an injectable, in situ-gelling hydrogel for the IM delivery of adipose-derived stem/stromal cells (ASCs), specifically designed to withstand the dynamic loading conditions of the lower limb and facilitate cytokine release from encapsulated cells...
January 4, 2018: Biomaterials
https://www.readbyqxmd.com/read/29322631/cold-inducible-rna-binding-protein-in-cancer-and-inflammation
#4
REVIEW
Daniel A Lujan, Joey L Ochoa, Rebecca S Hartley
RNA binding proteins (RBPs) play key roles in RNA dynamics, including subcellular localization, translational efficiency and metabolism. Cold-inducible RNA binding protein (CIRP) is a stress-induced protein that was initially described as a DNA damage-induced transcript (A18 hnRNP), as well as a cold-shock domain containing cold-stress response protein (CIRBP) that alters the translational efficiency of its target messenger RNAs (mRNAs). This review summarizes recent work on the roles of CIRP in the context of inflammation and cancer...
January 11, 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29317732/the-regulation-of-seventeen-inflammatory-mediators-are-associated-with-patient-outcomes-in-severe-fever-with-thrombocytopenia-syndrome
#5
Li-Fen Hu, Ting Wu, Bo Wang, Yuan-Yuan Wei, Qin-Xiang Kong, Ying Ye, Hua-Fa Yin, Jia-Bin Li
Severe fever with thrombocytopenia syndrome (SFTS) as an emerging infection disease results in high morbidity and mortality in China. In this study, the circulating levels of 36 inflammatory mediators in 33 SFTS patients on days 3-7, 8-12 and 13-20 post-illness were measured by a multiplex Luminex® system dynamically. Among the patients, 15 severe patients recovered, 11 severe patients died within three weeks. We found IL-1RA, IL-6, IL-15, IL-10, TNF-α, IFN-γ, G-CSF, eotaxin, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β and fractalkine were significantly upregulated in SFTS patients...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315416/human-induced-pluripotent-stem-cells-and-male-infertility-an-overview-of-current-progress-and-perspectives
#6
Fang Fang, Zili Li, Qian Zhao, Honggang Li, Chengliang Xiong
Recently, significant progress has been made in ART for the treatment of male infertility. However, current ART has failed to help infertile patients with non-obstructive azoospermia, unless donor sperm is used. In fact, most couples wish to have their own genetically related child. Human induced pluripotent stem cells (hiPSCs) can be generated from patients' somatic cells and in vitro derivation of functional germ cells from patient-specific iPSCs may provide new therapeutic strategies for infertile couples...
January 5, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29313961/impaired-phagocytosis-directs-human-monocyte-activation-in-response-to-fungal-derived-%C3%AE-glucan-particles
#7
Giorgio Camilli, Elif Eren, David L Williams, Vishukumar Aimanianda, Etienne Meunier, Jessica Quintin
Recognition of the fungal cell wall carbohydrate β-glucan by the host receptor Dectin-1 elicits broad immunomodulatory responses, such as phagocytosis and activation of oxidative burst. These responses are essential for engulfing and killing fungal pathogens. Phagocytic monocytes are key mediators of these early host inflammatory responses to infection. Remarkably, whether phagocytosis of fungal β-glucan leads to an inflammatory response in human monocytes remains to be established. Here, we show that phagocytosis of heat-killed Candida albicans is essential to trigger inflammation and cytokine release...
January 5, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29301935/-inhibition-of-histone-h3k27-demethylase-selectively-modulates-inflammatory-phenotypes-of-natural-killer-cells
#8
Adam Cribbs, Edward S Hookway, Graham Wells, Morten Lindow, Susanna Obad, Henrik Oerum, Rab K Prinjha, Nick Athanasou, Aneka Sowman, Martin Philpott, Henry Penn, Kalle Soderstrom, Marc Feldmann, Udo Oppermann
Natural killer (NK) cells are innate lymphocytes, important in immune surveillance and elimination of stressed, transformed, or virus-infected cells. They critically shape the inflammatory cytokine environment to orchestrate interactions of cells of the innate and adaptive immune systems. Some studies have reported that NK cell activation and cytokine secretion are controlled epigenetically, but have yielded only limited insight into the mechanisms. Using chemical screening with small-molecule inhibitors of chromatin methylation and acetylation, further validated by knockdown approaches, we here identified Jumonji-type histone H3K27 demethylases as key regulators of cytokine production in human NK cell subsets...
January 4, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29298542/the-role-of-alpha7-nicotinic-acetylcholine-receptors-in-inflammatory-bowel-disease-involvement-of-different-cellular-pathways
#9
Mohammad Seyedabadi, Reza Rahimian, Jean-Eric Ghia
Autonomic imbalance plays a pivotal role in the pathophysiology of inflammatory bowel diseases (IBD). The central nervous system (CNS) cooperates dynamically with the immune system to regulate inflammation through humoral and neural pathways. In particular, acetylcholine (Ach), the main neurotransmitter in the vagus nerve, decreases the production of pro-inflammatory cytokines through a mechanism dependent on the α7 nicotinic Ach receptors (α7nAChRs). Areas covered: Here, we review the evidence for involvement of the cholinergic anti-inflammatory pathway (CAP) in IBD...
January 3, 2018: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/29296744/substrate-bound-ccl21-and-icam1-combined-with-soluble-il-6-collectively-augment-the-expansion-of-antigen-specific-murine-cd4-t-cells
#10
Shimrit Adutler-Lieber, Irina Zaretsky, Helena Sabany, Elena Kartvelishvily, Ofra Golani, Benjamin Geiger, Nir Friedman
Immune processes within the complex microenvironment of the lymph node involve multiple intercellular, cell-matrix, and paracrine interactions, resulting in the expansion of antigen-specific T cells. Inspired by the lymph node microenvironment, we aimed to develop an ex vivo "synthetic immune niche" (SIN), which could effectively stimulate the proliferation of antigen-activated CD4+ T cells. This engineered SIN consisted of surfaces coated with the chemokine C-C motif ligand 21 (CCL21) and with the intercellular adhesion molecule 1 (ICAM1), coupled with the soluble cytokine interleukin 6 (IL-6) added to the culture medium...
June 27, 2017: Blood Advances
https://www.readbyqxmd.com/read/29295570/antibacterial-antiproliferative-and-immunomodulatory-activity-of-silver-nanoparticles-synthesized-with-fucans-from-the-alga-dictyota-mertensii
#11
Marília Medeiros Fernandes-Negreiros, Raynara Iusk Araújo Machado, Fabiana Lima Bezerra, Maria Celeste Nunes Melo, Monique Gabriela Chagas Faustino Alves, Luciana Guimaraes Alves Filgueira, Marcelo Antonio Morgano, Edvaldo Silva Trindade, Leandro Silva Costa, Hugo Alexandre Oliveira Rocha
In this study, we aimed to synthesize silver nanoparticles containing fucans from Dictyota mertensii (Martius) Kützing using an environmentally friendly method and to characterize their structure as well as antiproliferative, immunomodulatory, and antibacterial effects. Fucan-coated silver nanoparticles (FN) were characterized by Fourier-transform infrared analysis, dynamic light scattering, zeta potential, atomic force microscopy, energy dispersive X-ray spectroscopy, and inductively coupled plasma emission spectrometry...
December 25, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29289312/diffusion-of-cytokines-in-live-lymph-node-tissue-using-microfluidic-integrated-optical-imaging
#12
A E Ross, R R Pompano
Communication and drug efficacy in the immune system rely heavily on diffusion of proteins such as cytokines through the tissue matrix. Available methods to analyze diffusion in tissue require microinjection or saturating the tissue in protein, which may alter local transport properties due to damage or rapid cellular responses. Here, we developed a novel, user-friendly method - Microfluidic Integrated Optical Imaging (micro-IOI) - to quantify the effective diffusion coefficient of bioactive proteins in live tissue samples ex vivo...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29285415/preclinical-cancer-models-and-biomarkers-for-drug-development-new-technologies-and-emerging-tools
#13
Muthu Dhandapani, Aaron Goldman
Background and purpose: Predicting the efficacy of anticancer therapy is the holy grail of drug development and treatment selection in the clinic. To achieve this goal, scientists require pre-clinical models that can reliably screen anticancer agents with robust clinical correlation. However, there is increasing challenge to develop models that can accurately capture the diversity of the tumor ecosystem, and therefore reliably predict how tumors respond or resistant to treatment. Indeed, tumors are made up of a heterogeneous landscape comprising malignant cells, normal and abnormal stroma, immune cells, and dynamic microenvironment containing chemokines, cytokines and growth factors...
September 2017: Journal of Molecular Biomarkers & Diagnosis
https://www.readbyqxmd.com/read/29285394/c-c-motif-chemokine-receptors-in-gastric-cancer
#14
Hyewon Ryu, Seung Woo Baek, Ji Young Moon, In-Sook Jo, Nayoung Kim, Hyo Jin Lee
Gastric cancer is the fifth most common cancer and the third leading cause of cancer-associated mortality worldwide. Despite recent advances in molecular and clinical research, patients with gastric cancer at an advanced stage have a dismal prognosis and poor survival rates, and systemic treatment relies predominantly on traditional cytotoxic chemotherapy. To improve patients' quality of life and survival, an improved understanding of the complex molecular mechanisms involved in gastric cancer progression and treatment resistance, and of its clinical application in the development of novel targeted therapies, is urgently required...
January 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29284398/mechanisms-of-radiation-bystander-and-non-targeted-effects-implications-to-radiation-carcinogenesis-and-radiotherapy
#15
Rasoul Yahyapour, Elahe Motevaseli, Abolhasan Rezaeyan, Hamid Abdollahi, Bagher Farhood, Mohsen Cheki, Masoud Najafi, Vilmar Villa
Knowledge of radiobiology is of paramount importance to be able to grasp and have an in-depth understanding of the consequences of ionizing radiation. One of the most important effects of this physical stressor's interaction to targeted and non-targeted cells, tissues and organs is on the late effects on the development of primary and secondary cancers. Thus, an in-depth understanding of the mechanisms of radiation carcinogenesis remain to be elucidated, and some studies have demonstrated or proposed a role of non-targeted effect in excess risk of cancer incidence...
December 29, 2017: Current Radiopharmaceuticals
https://www.readbyqxmd.com/read/29283192/functional-and-phenotypical-analysis-of-il-6-secreting-cd4-t-cells-in-human-adipose-tissue
#16
Anja J de Jong, Sabrina Pollastro, Joanneke C Kwekkeboom, Stefan N Andersen, Annemarie L Dorjée, Aleida M Bakker, Fawaz Alzaid, Antoine Soprani, Rob G H H Nelissen, Jan B Mullers, Nicolas Venteclef, Niek de Vries, Margreet Kloppenburg, René E M Toes, Andreea Ioan-Facsinay
Emerging evidence indicates that a dynamic interplay between the immune system and adipocytes contributes to the disturbed homeostasis in adipose tissue of obese subjects. Recently, we observed IL-6-secretion by CD4+ T cells from the stromal vascular fraction (SVF) of the infrapatellar fat pad (IFP) of knee osteoarthritis patients directly ex vivo. Here we show that human IL-6+ CD4+ T cells from SVF display a more activated phenotype than the IL-6- T cells, as evidenced by the expression of the activation marker CD69...
December 28, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29277546/inhibition-of-apoptosis-signal-regulating-kinase-1-attenuates-myocyte-hypertrophy-and-fibroblast-collagen-synthesis
#17
Wendi Yang, Bing Hui Wang, Ian Wang, Li Huang, Feby Savira, Andrew Kompa, Beat M Jucker, Robert N Willette, Darren Kelly, Henry Krum, Zhiliang Li, Qian Fu
BACKGROUND: Cardiac remodelling is a dynamic process whereby structural and functional changes occur within the heart in response to injury or inflammation. Recent studies have demonstrated reactive oxygen species sensitive MAPK, apoptosis signal-regulating kinase 1 (ASK1) plays a critical role in cardiac remodelling. This study aims to determine the effectiveness of small molecule ASK1 inhibitors on these processes and their therapeutic potential. METHODS: Neonatal rat cardiac fibroblasts (NCF) were pre-treated with ASK1 inhibitors, G2261818A (G226) and G2358939A (G235), for 2hours before stimulated with 100nM angiotensin II (AngII), 10μM indoxyl sulphate (IS) or 10ng/ml transforming growth factor β1 (TGFβ1) for 48hours...
December 14, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/29276513/multidimensional-analysis-integrating-human-t-cell-signatures-in-lymphatic-tissues-with-sex-of-humanized-mice-for-prediction-of-responses-after-dendritic-cell-immunization
#18
Valery Volk, Andreas I Reppas, Philippe A Robert, Loukia M Spineli, Bala Sai Sundarasetty, Sebastian J Theobald, Andreas Schneider, Laura Gerasch, Candida Deves Roth, Stephan Klöss, Ulrike Koehl, Constantin von Kaisenberg, Constanca Figueiredo, Haralampos Hatzikirou, Michael Meyer-Hermann, Renata Stripecke
Mice transplanted with human cord blood-derived hematopoietic stem cells (HSCs) became a powerful experimental tool for studying the heterogeneity of human immune reconstitution and immune responses in vivo. Yet, analyses of human T cell maturation in humanized models have been hampered by an overall low immune reactivity and lack of methods to define predictive markers of responsiveness. Long-lived human lentiviral induced dendritic cells expressing the cytomegalovirus pp65 protein (iDCpp65) promoted the development of pp65-specific human CD8+ T cell responses in NOD...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29250067/group-2-innate-lymphoid-cells-exhibit-a-dynamic-phenotype-in-allergic-airway-inflammation
#19
Bobby W S Li, Ralph Stadhouders, Marjolein J W de Bruijn, Melanie Lukkes, Dior M J M Beerens, Maarten D Brem, Alex KleinJan, Ingrid Bergen, Heleen Vroman, Mirjam Kool, Wilfred F J van IJcken, Tata Nageswara Rao, Hans Jörg Fehling, Rudi W Hendriks
Group 2 innate lymphoid cells (ILC2) are implicated in allergic asthma as an early innate source of the type 2 cytokines IL-5 and IL-13. However, their induction in house dust mite (HDM)-mediated airway inflammation additionally requires T cell activation. It is currently unknown whether phenotypic differences exist between ILC2s that are activated in a T cell-dependent or T cell-independent fashion. Here, we compared ILC2s in IL-33- and HDM-driven airway inflammation. Using flow cytometry, we found that surface expression levels of various markers frequently used to identify ILC2s were dependent on their mode of activation, highly variable over time, and differed between tissue compartments, including bronchoalveolar lavage (BAL) fluid, lung, draining lymph nodes, and spleen...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29248120/infection-of-hematopoietic-stem-cells-by-leishmania-infantum-increases-erythropoiesis-and-alters-the-phenotypic-and-functional-profiles-of-progeny
#20
Raquel Carvalho-Gontijo, Diana Raquel Moreira, Mariana Resende, Matheus Fernandes Costa-Silva, Vanessa Peruhype-Magalhães, Cláudia Maria Franco Ribeiro, Daniel Dias Ribeiro, Ricardo Silvestre, Anabela Cordeiro-da-Silva, Olindo Assis Martins-Filho, Andréa Teixeira-Carvalho
Immunosuppression is a well-established risk factor for Visceral Leishmaniasis. Post-immunosuppression leishmaniasis is characterized by an increase of parasite burden, hematopoietic disorders and unusual clinical manifestations. Although there are many reports on bone marrow findings in VL, less is known about the relationship between parasite dynamics in this organ and the function of either hematopoietic stem cells and progenitor cells themselves. In the present study, we tackle these issues using a new approach of infecting human stem cells derived from bone marrow with L...
November 7, 2017: Cellular Immunology
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