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mirna aneurysm

Crystal Yin Tung Chan, Bernice Lai Yee Cheuk, Stephen Wing Keung Cheng
OBJECTIVE: MicroRNAs (miRNAs or miRs) have been highlighted to be involved in abdominal aortic aneurysm (AAA) with the emergence of recent miRNA microarray profiling studies. miR-516a-5p has been shown to be significantly overexpressed in vascular smooth muscle cells (VSMCs) from human AAA tissues from our previous microarray study, suggesting its crucial association with AAA. In addition, further bioinformatics analysis predicted methylenetetrahydrofolate reductase (MTHFR), which regulates homocysteine (Hcy) metabolism and is proposed to be a risk gene for AAA formation, to be the down-regulation target of miR-516a-5p...
March 10, 2017: Annals of Vascular Surgery
Liqian Sun, Manman Zhao, Jingbo Zhang, Ming Lv, Youxiang Li, Xinjian Yang, Aihua Liu, Zhongxue Wu
BACKGROUND/AIMS: Our previous microarray results identified numerous microRNAs (miRNAs), including miR-29b, that were differentially expressed in the serum of intracranial aneurysm (IA) patients. The current study aimed to investigate whether miR-29b downregulation in IA could promote the phenotypic modulation of vascular smooth muscle cells (VSMCs) involved in the pathogenesis of aneurysm by activating ATG14-mediated autophagy. METHODS: First, the levels of miR-29b and autophagy related genes (ATGs) between IA patients and normal subjects were compared...
January 30, 2017: Cellular Physiology and Biochemistry
Peng Gao, Jiyuan Si, Bin Yang, Jixiang Yu
BACKGROUND The objective of the present study was to identify the association between miR-15a-5p and CDKN2B, and their roles in regulating the development of abdominal aortic aneurysm (AAA). MATERIAL AND METHODS We searched the miRNA database online ( and used a luciferase reporter assay system to study the regulatory relationship between miR-15a-5p and CDKN2B. We also conducted real-time PCR and Western blot analysis to study the mRNA and protein expression level of CDKN2B among different patient groups (participants with abdominal aortic aneurysm (AAA) and normal controls) or cells treated with scramble control, miR-15a-5p mimics, CDKN2B siRNA, and miR-15a-5p inhibitors...
February 18, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Azra Alajbegovic, Karolina M Turczyńska, Tran Thi Hien, Pilar Cidad, Karl Swärd, Per Hellstrand, Alessandro Della Corte, Amalia Forte, Sebastian Albinsson
The dynamic properties of the actin cytoskeleton in smooth muscle cells play an important role in a number of cardiovascular disease states. The state of actin does not only mediate mechanical stability and contractile function but can also regulate gene expression via myocardin related transcription factors (MRTFs). These transcriptional co-activators regulate genes encoding contractile and cytoskeletal proteins in smooth muscle. Regulation of small non-coding microRNAs (miRNAs) by actin polymerization may mediate some of these effects...
December 8, 2016: Biochimica et Biophysica Acta
Jing Luo, Hengwei Jin, Yuhua Jiang, Huijian Ge, Jiwei Wang, Youxiang Li
BACKGROUND MiR-9 is reportedly involved with many diseases, such as acute myeloid leukemia and liver oncogenesis. In the present study we investigated the molecular mechanism, including the potential regulator and signaling pathways, of MYOCD, which is the gene that in humans encodes the protein myocardin. MATERIAL AND METHODS We searched the online miRNA database ( with the "seed sequence" located within the 3'-UTR of the target gene, and then validated MYOCD to be the direct gene via luciferase reporter assay system, and further confirmed it in cultured cells by using Western blot analysis and realtime PCR...
November 8, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Hao Li, Wen Wang, Lu Zhang, Qing Lan, Jiangfei Wang, Yong Cao, Jizong Zhao
BACKGROUND: Intracranial aneurysm (IA) is a cerebrovascular disorder characterized by an abnormally bulged artery in the brain and subarachnoid hemorrhage caused by IA rupture with a high ratio of fatality and morbidity. However, the genetic cause of IA remains largely unknown. METHODS: A dysregulated long noncoding RNA (lncRNA) -associated competing endogenous RNA (ceRNA) network was constructed by using sample-matched microRNA (miRNA), lncRNA, and messenger (mRNA) expression profiles in 12 saccular IA samples and paired control arteries...
January 2017: World Neurosurgery
Yiming Zhang, Zhao Liu, Min Zhou, Changjian Liu
Aberrant smooth muscle cells (SMCs) play important roles in the formation of abdominal aortic aneurysm (AAA). Although the molecular mechanism of AAA formation has been investigated, there is a lack of understanding concerning the role of microRNAs (miRNAs) in AAA, which the current study aimed to address. Firstly, miRNA array analysis was performed in order to compare the miRNA profiles in a mouse model of AAA with those in normal control mice, and differentially expressed miRNAs were identified. miR-129-5p was selected for further analysis, and was used to transfect human SMCs...
October 2016: Experimental and Therapeutic Medicine
Sabina Licholai, Wojciech Szczeklik, Marek Sanak
BACKGROUND: Abdominal aortic aneurysm (AAA) is usually asymptomatic and mostly diagnosed incidentally. Aortic dilatation progresses along with the wall weakening and eventually leads to a life-threatening rupture. Currently, there are no effective screening biomarkers for AAA. MicroRNAs, a class of small noncoding RNA molecules, offer great potential as biomarkers because of their stability in the bloodstream and expression profile specific for different diseases. OBJECTIVE: To assess the circulating miR-29c-3p as a potential biomarker of AAA and to elucidate the biological functions of miR-29c-3p in terms of pathogenesis of aneurysms...
2016: MicroRNA
Albert Busch, Suzanne M Eken, Lars Maegdefessel
Non-coding RNA (ncRNA) is a class of genetic, epigenetic and translational regulators, containing short and long transcripts with intriguing abilities for use as biomarkers due to their superordinate role in disease development. In the past five years many of these have been investigated in cardiovascular diseases (CVD), mainly myocardial infarction (MI) and heart failure. To extend this view, we summarize the existing data about ncRNA as biomarker in the whole entity of CVDs by literature-based review and comparison of the identified candidates...
June 2016: Annals of Translational Medicine
Stanley S Stylli, Alexios A Adamides, Rachel M Koldej, Rodney B Luwor, David S Ritchie, James Ziogas, Andrew H Kaye
OBJECTIVE MicroRNAs (miRNAs) regulate gene expression and therefore play important roles in many physiological and pathological processes. The aim of this pilot study was to determine the feasibility of extraction and subsequent profiling of miRNA from CSF samples in a pilot population of aneurysmal subarachnoid hemorrhage patients and establish if there is a distinct CSF miRNA signature between patients who develop cerebral vasospasm and those who do not. METHODS CSF samples were taken at various time points during the clinical management of a subset of SAH patients (SAH patient samples without vasospasm, n = 10; SAH patient samples with vasospasm, n = 10)...
April 29, 2016: Journal of Neurosurgery
Kimon Bekelis, Joanna S Kerley-Hamilton, Amy Teegarden, Craig R Tomlinson, Rachael Kuintzle, Nathan Simmons, Robert J Singer, David W Roberts, Manolis Kellis, David A Hendrix
OBJECTIVE The molecular mechanisms behind cerebral aneurysm formation and rupture remain poorly understood. In the past decade, microRNAs (miRNAs) have been shown to be key regulators in a host of biological processes. They are noncoding RNA molecules, approximately 21 nucleotides long, that posttranscriptionally inhibit mRNAs by attenuating protein translation and promoting mRNA degradation. The miRNA and mRNA interactions and expression levels in cerebral aneurysm tissue from human subjects were profiled...
December 2016: Journal of Neurosurgery
Xian Wei Su, Anna Ho Yin Chan, Gang Lu, Marie Lin, Johnny Sze, Jing Ye Zhou, Wai Sang Poon, Qiang Liu, Vera Zhi Yuan Zheng, George Kwok Chu Wong
BACKGROUND: Aneurysmal subarachnoid hemorrhage (SAH) is a highly morbid and fatal condition with high rate of cognitive impairment and negative impact in quality of life among survivors. Delayed cerebral infarction (DCI) is one the major factors for these negative outcomes. In this study we compared the circulating microRNA profiles of SAH patients and healthy individuals, and the circulating microRNA profiles of SAH patients with and without DCI. METHODS: Peripheral blood samples on Day 7 after the onset of SAH were subjected to microarray analysis with Affymetrix miRNA 3...
2015: PloS One
Ondřej Volný, Linda Kašičková, Dominika Coufalová, Petra Cimflová, Jan Novák
Cardiovascular diseases are major causes of morbidity and mortality in developed countries. Cerebrovascular diseases, especially stroke, represent major burden of disability and economy impact. Major advances in primary and secondary prevention and therapy are needed in order to tackle this public health problem. Our better understanding of pathophysiology is essential in order to develop novel diagnostic and therapeutic tools and strategies. microRNAs are a family of important post-transcriptional regulators of gene expression and their involvement in the pathophysiology of cerebrovascular diseases has already been reported...
2015: Advances in Experimental Medicine and Biology
André Jakob, Jane Whelan, Matthaeus Kordecki, Reinhard Berner, Brigitte Stiller, Raoul Arnold, Rudiger von Kries, Elena Neumann, Nicholas Roubinis, Mirna Robert, Jochen Grohmann, René Höhn, Markus Hufnagel
BACKGROUND: National estimates of Kawasaki disease (KD) incidence often do not include incomplete cases (diagnosed based on only laboratory or echocardiographic criteria), and/or they rely on retrospective case reports and data registries where underreporting is known to be a problem. METHODS: We conducted a prospective nationwide KD surveillance study in children younger than 5 years through the hospital-based German Pediatric Surveillance Unit (ESPED). We accounted for underreporting through applying capture-recapture methodology in 2 federal states using hospital discharge records with KD International Statistical Classification of Diseases and Related Health Problems 10th revision code (ie, M30...
February 2016: Pediatric Infectious Disease Journal
Xian-ming Fu, Yang-zhao Zhou, Zhao Cheng, Xiao-bo Liao, Xin-min Zhou
An aortic aneurysm (AA) is a common disease with potentially life-threatening complications. Despite significant improvements in the diagnosis and treatment of AA, the associated morbidity and mortality remain high. MicroRNAs (miRNAs, miR) are small noncoding ribonucleic acids that negatively regulate gene expression at the posttranscriptional level by inhibiting mRNA translation or promoting mRNA degradation. miRNAs are recently reported to be critical modulators for vascular cell functions such as cell migration, contraction, differentiation, proliferation, and apoptosis...
2015: BioMed Research International
X T Sima, J G Xu, J Li, C You
Vascular inflammation has been shown to be involved in the pathogenesis of intracranial aneurysms (IA). MiRNAs are key molecules that participate in the regulation of many important biological processes including inflammation. Studies on the hsa-miR-146a rs2910164 polymorphism and its association with different inflammatory related diseases have engendered inconsistent results, and until now, there have been no reports on the association between this polymorphism and the susceptibility to IA. In this study, we aimed to investigate whether the rs2910164 polymorphism is involved in the process of IA...
2015: Genetics and Molecular Research: GMR
Athanassios Kotsinas, Fragkiska Sigala, Spiros D Garbis, Giorgos Galyfos, Konstantinos Filis, Konstantinos Vougas, Alexandros Papalampros, Elizabeth E Johnson, Efstathios Chronopoulos, Alexandros G Georgakilas, Vassilis G Gorgoulis
Cardiovascular diseases (CVDs) have become a predominant cause of death worldwide. Although CVDs encompass a variety of pathological conditions that affect the heart and blood vasculature, they can be classified into two major groups according to their basic pathophysiologic mechanisms, namely atherosclerosis and aneurysm formation. Increasing evidence implicates micro-RNAs (miRNAs or miRs) as vital factors both in the initiation and progression processes of CVDs. Some miRs have a promoting role in CVD development, while others have a protective role...
2015: Current Medicinal Chemistry
Rong Wei, Rufang Zhang, Yewei Xie, Li Shen, Fang Chen
BACKGROUND & AIMS: Deep hypothermic circulatory arrest (DHCA) is a cerebral protection technique that has been used in the operations involving the aortic arch and brain aneurysm for decades. We previous showed that DHCA treated rats developed a significant oxidative stress and apoptosis in neurons. We here intend to investigate the protective the effect of hydrogen against oxidative stress-induced cell injury and the involved mechanisms using an in vitro experimental model of hypoxia/reoxygenation (H/R) on HT-22 cells...
2015: Cellular Physiology and Biochemistry
Rafaelle Spear, Ludovic Boytard, Renaud Blervaque, Maggy Chwastyniak, David Hot, Jonathan Vanhoutte, Bart Staels, Yves Lemoine, Nicolas Lamblin, François-René Pruvot, Stephan Haulon, Philippe Amouyel, Florence Pinet
Abdominal aortic aneurysm (AAA) is an inflammatory disease associated with marked changes in the cellular composition of the aortic wall. This study aims to identify microRNA (miRNA) expression in aneurysmal inflammatory cells isolated by laser microdissection from human tissue samples. The distribution of inflammatory cells (neutrophils, B and T lymphocytes, mast cells) was evaluated in human AAA biopsies. We observed in half of the samples that adventitial tertiary lymphoid organs (ATLOs) with a thickness from 0...
2015: International Journal of Molecular Sciences
Søren Bache, Rune Rasmussen, Maria Rossing, Niels Risør Hammer, Marianne Juhler, Lennart Friis-Hansen, Finn Cilius Nielsen, Kirsten Møller
BACKGROUND: Secondary brain injury accounts for a major part of the morbidity and mortality in patients with spontaneous aneurysmal subarachnoid hemorrhage (SAH), but the pathogenesis and pathophysiology remain controversial. MicroRNAs (miRNAs) are important posttranscriptional regulators of complementary mRNA targets and have been implicated in the pathophysiology of other types of acute brain injury. Cerebral microdialysis is a promising tool to investigate these mechanisms. We hypothesized that miRNAs would be present in human cerebral microdialysate...
May 7, 2015: Journal of Translational Medicine
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