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Antibiotic protocols

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https://www.readbyqxmd.com/read/28816710/establishment-of-primary-cell-culture-from-ascitic-fluid-and-solid-tumor-obtained-from-epithelial-ovarian-carcinoma-patients
#1
Rajarshi Kar, Diwesh Chawla, Bindiya Gupta, Mohit Mehndiratta, Neelam Wadhwa, Rachna Agarwal
OBJECTIVE: Ovarian cancer is the seventh leading cause of cancer death worldwide. This is mainly due to late diagnosis and high rate of relapse and resistance following chemotherapy. In the present study, we describe simple and cost-effective method to establish primary culture from ascitic fluid and solid tumor obtained from epithelial ovarian carcinoma patient, which may provide a better tool for in vitro testing of drug sensitivity and designing individualized treatment protocol. METHODS: Complete Dulbecco modified Eagle medium (DMEM) was prepared by supplementing DMEM with 10% fetal bovine serum and antibiotics (ciprofloxacin and amphotericin B)...
August 16, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28816443/characterization-of-ciprofloxacin-permeation-pathways-across-the-porin-ompc-using-metadynamics-and-a-string-method
#2
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
The rapid spreading of antimicrobial resistance in Gram-negative bacteria has become a major threat for humans as well as animals. As one of the main factors involved, the permeability of the outer membrane has attracted quite some attention recently. However, the knowledge regarding the translocation mechanisms for most available antibiotics is so far rather limited. Here, a theoretical study concerning the diffusion route of ciprofloxacin across the outer membrane porin OmpC from E. coli is presented. To this end, we establish a protocol to characterize meaningful permeation pathways by combining metadynamics with the zero-temperature string method...
August 17, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28811792/antimicrobial-susceptibility-patterns-of-clinical-isolates-of-pseudomonas-aeruginosa-isolated-from-patients-of-respiratory-tract-infections-in-a-tertiary-care-hospital-peshawar
#3
Abdul Samad, Tanveer Ahmed, Afaq Rahim, Abdul Khalil, Iftikhar Ali
OBJECTIVE: This study aims to determine the prevalence and susceptibility pattern of Pseudomonas aeruginosa and multidrug-resistant (MDR) isolates in patients suffering from respiratory tract infection. METHODS: A cross sectional study was conducted from January to December 2014 in Northwest General Hospital and Research Centre, Peshawar. A total of 615 sputum samples were collected from both in and out-patients. Sputum samples were collected as per standard procedure and were inoculated on Blood, MacConkey and Chocolate agar...
May 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28811266/expression-and-purification-of-a-difficult-sarcomeric-protein-telethonin
#4
Huanbo Tan, Wencheng Su, Pengju Wang, Wenyu Zhang, Michael Sattler, Peijian Zou
Telethonin anchors the N-terminal region of titin in the Z-disk of the sarcomere by binding to two immunoglobulin-like (Ig) domains (Z1 and Z2) of titin (Z1Z2). Thereby telethonin plays an important role in myofibril assembly and in muscle development and functional regulation. The expression and purification of recombinant telethonin is very challenging. In previous studies, recombinant telethonin expressed from E. coli was refolded in the presence of Z1Z2. Here, we report various strategies to establish a reliable and efficient protocol for the preparation of telethonin and titin Z1Z2 protein...
August 12, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28810197/photochemical-formation-of-chitosan-stabilized-near-infrared-absorbing-silver-nanoworms-a-green-synthetic-strategy-and-activity-on-gram-negative-pathogenic-bacteria
#5
Sreekar Marpu, Samar S Kolailat, Daniel Korir, Brian L Kamras, Ratnesh Chaturvedi, Abel Joseph, Christopher M Smith, Misael C Palma, Jyoti Shah, Mohammad A Omary
A facile, single-step, non-seeded photochemical protocol for producing a new type of anisotropic silver nanostructure, "nanoworms", with curved longer dimensions and smooth, rounded edges. The nanoworms exhibit surface plasmon resonance (SPR) absorption in the near-infrared window (NIRW) region and are stabilized using biocompatible polymer chitosan, rendering biocompatibility and amplified safety for biological utility of the composition. Both NIRW-absorbing nanoworms and visible-absorbing nanospheres herein are attained exclusively by employing green chemistry principles...
August 4, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28810038/culture-positive-endophthalmitis-after-open-globe-injuries-with-and-without-retained-intraocular-foreign-bodies
#6
Tanuj P Banker, Andrew J McClellan, Benjamin D Wilson, Fabiana M Juan, Ajay E Kuriyan, Nidhi Relhan, Felix V Chen, Eric D Weichel, Thomas A Albini, Audina M Berrocal, Jay Sridhar, Ninel Z Gregori, Justin H Townsend, Harry W Flynn
BACKGROUND AND OBJECTIVE: To report the visual and anatomical outcomes and microbiologic spectrum of culture-positive endophthalmitis in open globe injuries (OGIs) with or without intraocular foreign bodies (IOFBs). PATIENTS AND METHODS: A retrospective, interventional case series of OGIs (n = 718) treated between 2004 and 2015. Patients underwent a management protocol for OGI, including systemic broad-spectrum antibiotics, on presentation. RESULTS: Culture-positive cases of endophthalmitis after open globe repair occurred in 2...
August 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28809612/a-retrospective-study-evaluating-the-efficacy-of-identification-and-management-of-sepsis-at-a-district-level-hospital-internal-medicine-department-in-the-western-cape-province-south-africa-in-comparison-with-the-guidelines-stipulated-in-the-2012-survivi
#7
Raisa Bhikoo, Sarah Versfeld, M M De V Basson, Almero H Oosthuizen
BACKGROUND: Currently there is little information on the identification, management and outcomes of patients with sepsis in developing countries. Simple cost-effective measures such as accurate identification of patients with sepsis and early antibiotic administration are achievable targets, within reach without having to make use of unsustainable protocols constructed in developed countries. OBJECTIVES: To assess the ability of clinicians at a district-level hospital to identify and manage sepsis, and to assess patient outcome in terms of in-hospital mortality and length of hospital stay given the above management...
July 28, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28807915/both-14-day-hybrid-and-bismuth-quadruple-therapies-cure-most-patients-with-helicobacter-pylori-infection-in-populations-with-moderate-antibiotic-resistance-a-randomized-controlled-trial
#8
Feng-Woei Tsay, Deng-Chyang Wu, Hsien-Chung Yu, Sung-Shuo Kao, Kung-Hung Lin, Jin-Shiung Cheng, Huay-Min Wang, Wen-Chi Chen, Wei-Chih Sun, Kuo-Wang Tsai, Ping-I Hsu
BACKGROUND & AIM: Hybrid therapy is a novel two-step treatment achieving a high eradication rate for H. pylori infection. Currently, whether this new therapy achieves a higher eradication rate than bismuth quadruple therapy remains an unanswered question. The aim of this prospective, randomized, comparive study was to investigate the efficacies of 14-day hybrid therapy and bismuth quadruple therapy in the treatment of H. pylori infection.METHODS: From July 2013 to June 2015, eligible H. pylori-infected subjects were randomly assigned to receive either 14-day bismuth quadruple therapy (pantoprazole, bismuth subcitrate, tetracycline, and metronidazole for 14 days) or 14-day hybrid therapy (a 7-day dual therapy with pantoprazole plus amoxicillin, followed by a 7-day quadraple therapy with pantoprazole plus amoxicillin, clarithromycin and metronidazole)...
August 14, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28807380/reconstruction-after-osteomyelitis-of-the-midfoot-from-high-pressure-washer-injury
#9
Matthew M Reiner, Wissam E Khoury, Audley Mackel, Duane J Ehredt, Mark C Razzante
Reports of ballistic injures to the extremities typically include those involving gunshot wounds. However, high-pressure washer injuries frequently lead to equally damaging injuries. These injuries should not be overlooked, because they can result in high morbidity and complication rates. Similar to the open fracture protocol, these injuries require prompt debridement and irrigation with administration of antibiotics to avoid limb loss. The present case report identified a delay in the treatment protocol that left limited options for the patient...
August 11, 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/28807192/does-compliance-with-antibiotic-prophylaxis-in-pediatric-simple-appendicitis-matter
#10
Krislynn M Mueck, Luke R Putnam, Kathryn T Anderson, Kevin P Lally, KuoJen Tsao, Lillian S Kao
BACKGROUND: Institutional protocols for preincisional antibiotic prophylaxis can standardize care and improve outcomes. However, challenges remain in compliance with such protocols for urgent or emergent operations. We hypothesized that compliance with an institutional protocol for antibiotic prophylaxis for appendectomy for appendicitis in pediatric patients results in reduced surgical site infections (SSIs) after simple appendectomy. METHODS: This retrospective study assessed all pediatric patients (≤18 y) who underwent appendectomy for confirmed simple appendicitis at a tertiary children's hospital between 2012 and 2015...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28803905/azithromycin-treatment-is-able-to-control-the-infection-by-two-genotypes-of-toxoplasma-gondii-in-human-trophoblast-bewo-cells
#11
Mayara Ribeiro, Priscila Silva Franco, Janice Buiate Lopes-Maria, Mariana Bodini Angeloni, Bellisa de Freitas Barbosa, Angelica de Oliveira Gomes, Andressa Silva Castro, Rafaela José da Silva, Fernanda Chaves de Oliveira, Iliana Claudia Balga Milian, Olindo Assis Martins-Filho, Francesca Ietta, José Roberto Mineo, Eloisa Amália Vieira Ferro
Trophoblast infection by Toxoplasma gondii plays a pivotal role in the vertical transmission of toxoplasmosis. Here, we investigate whether the antibiotic therapy with azithromycin, spiramycin and sulfadiazine/pyrimethamine are effective to control trophoblast infection by two Brazilian T. gondii genotypes, TgChBrUD1 or TgChBrUD2. Two antibiotic protocols were evaluated, as follow: i) pre-treatment of T. gondii-tachyzoites with selected antibiotics prior trophoblast infection and ii) post-treatment of infected trophoblasts...
August 10, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28802424/can-antibiotic-impregnated-cement-nail-achieve-both-infection-control-and-bony-union-in-infected-diaphyseal-femoral-non-unions
#12
Chetan Pradhan, Atul Patil, Chetan Puram, Dheeraj Attarde, Parag Sancheti, Ashok Shyam
BACKGROUND: Infected non-union is complex and debilitating disorder affecting orthopaedic surgeon and patient in terms of cost and time. Many methods are described in the literature for treatment of infected non-union. Local high concentration of antibiotic and mechanical stability of antibiotic cement impregnated intramedullary nail (ACIIN) proves cost and time effective. Recently it was suggested that ACIIN can achieve both union and infection control in infected non-unions with bone gap less than 4cm...
August 2017: Injury
https://www.readbyqxmd.com/read/28802354/a-novel-management-of-streptococcal-pharyngotonsillar-infections-by-laser-activated-silver-nanoparticles-and-methylene-blue-conjugate-in%C3%A2-vitro-study
#13
Ahmed Kassab, Ola Dabous, Mona Morsy
INTRODUCTION: Species of Streptococcus are classified based on their hemolytic properties into alpha and beta types. And, since antimicrobial drug resistance is an increasingly problematic issue, the efforts to develop modalities that would overcome this obstacle and avoid antibiotic side effects is an ongoing challenge. METHODS AND MATERIALS: 20 patients from both sexes were selected. The isolated organisms were identified according to standard laboratory methods...
September 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28801434/comparative-effectiveness-of-six-chinese-herb-formulas-for-acute-exacerbation-of-chronic-obstructive-pulmonary-disease-protocol-for-systematic-review-and-network-meta-analysis
#14
Shaonan Liu, Jing Chen, Yihan He, Lei Wu, Jiaqi Lai, Jinhong Zuo, Lihong Yang, Xinfeng Guo
INTRODUCTION: Chinese medicine is commonly used to combine with pharmacotherapy for the treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Six Chinese herb formulas involving Weijing decoction, Maxingshigan decoction, Yuebijiabanxia decoction, Qingqihuatan decoction, Dingchuan decoction and Sangbaipi decoction are recommended in Chinese medicine clinical guideline or textbook, to relieve patients with phlegm-heat according to Chinese syndrome differentiation...
August 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28796008/methicillin-resistant-staphylococcus-aureus-in-cystic-fibrosis-how-should-it-be-managed
#15
Marianne S Muhlebach
PURPOSE OF REVIEW: Methicillin-resistant Staphylococcus aureus (MRSA) remains prevalent in people with cystic fibrosis (CF). As chronic infection with worse pulmonary outcomes develops frequently, the organism is concerning to CF providers and patients. This review describes current epidemiology, our understanding of risk factors for MRSA infection, and relevant aspects of treatment with review of new and ongoing trials. RECENT FINDINGS: Prevalence ranges from a low of 3 to 4% in some European countries to a high of approximately 26% in the United States...
August 8, 2017: Current Opinion in Pulmonary Medicine
https://www.readbyqxmd.com/read/28794875/the-humtick-study-protocol-for-a-prospective-cohort-study-on-post-treatment-lyme-disease-syndrome-and-the-disease-and-cost-burden-of-lyme-borreliosis-in-belgium
#16
Laurence Geebelen, Tinne Lernout, Benoît Kabamba-Mukadi, Veroniek Saegeman, Hein Sprong, Steven Van Gucht, Philippe Beutels, Niko Speybroeck, Katrien Tersago
BACKGROUND: In Belgium, different routine surveillance systems are in place to follow-up Lyme borreliosis trends. However, accurate data on the disease and monetary burden for the different clinical manifestations are lacking. Despite recommended antibiotic treatment, a proportion of Lyme patients report persisting aspecific symptoms for six months or more (e.g. fatigue, widespread musculoskeletal pain, cognitive difficulties), a syndrome now named "post-treatment Lyme disease syndrome" (PTLDS)...
2017: Archives of Public Health, Archives Belges de Santé Publique
https://www.readbyqxmd.com/read/28791879/management-of-colon-perforation-during-percutaneous-nephrolithotomy-12-years-of-experience-in-a-referral-center
#17
Robab- Maghsoudi, Massoud Etemadian, Amir Hossein Kashi, Kaveh Mehravaran
INTRODUCTION: The management protocol for colon perforation during percutaneous nephrolithotomy (PNL) is controversial due to the scarcity of reported cases and their management diversity. We present our management experience of colon perforation during PNL. MATERIALS AND METHODS: All PNL operations between April 2004 and September 2016 in our center (N=11376) were reviewed for the occurrence and management of colon perforation. We typically performed PNL with insertion of nephrostomy tube before mid 2007...
August 9, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28791235/recurrent-urinary-tract-infections-in-patients-with-incomplete-bladder-emptying-is-there-a-role-for-intravesical-therapy
#18
REVIEW
Elizabeth V Dray, J Quentin Clemens
The goal of this review article is to discuss the etiology of recurrent urinary tract infections (UTIs) in individuals with impaired bladder emptying, evaluate existing studies regarding UTI prevention strategies in this population, and explore the published experiences with intravesical therapy for the prevention and treatment of recurrent UTIs in patients performing clean intermittent catheterization (CIC). We will also describe the intravesical antibiotic protocol utilized at our institution.
July 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28790976/factors-affecting-measurement-of-salivary-cortisol-and-secretory-immunoglobulin-a-in-field-studies-of-athletes
#19
Barry Thomas Pritchard, Warren Stanton, Roger Lord, Peter Petocz, Gert-Jan Pepping
AIMS: Biological and lifestyle factors, such as daily rhythm, caffeine ingestion, recent infection, and antibiotic intake, have been shown to influence measurements of salivary cortisol (SC) and secretory immunoglobulin A (sIgA). Current methodology in unsynchronized, field-based biomarker studies does not take these effects into account. Moreover, very little is known about the combined effects of biological and lifestyle factors on SC and sIgA. This study supports development of a protocol for measuring biomarkers from saliva collected in field studies by examining the individual and combined effects of these factors on SC and sIgA...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28790042/multicentre-randomised-controlled-trial-to-investigate-the-usefulness-of-continuous-pneumatic-regulation-of-tracheal-cuff-pressure-for-reducing-ventilator-associated-pneumonia-in-mechanically-ventilated-severe-trauma-patients-the-agate-study-protocol
#20
Nicolas Marjanovic, Denis Frasca, Karim Asehnoune, Catherine Paugam, Sigismond Lasocki, Carole Ichai, Jean-Yves Lefrant, Marc Leone, Claire Dahyot-Fizelier, Julien Pottecher, Dominique Falcon, Benoit Veber, Jean-Michel Constantin, Sabrina Seguin, Jérémy Guénézan, Olivier Mimoz
INTRODUCTION: Severe trauma represents the leading cause of mortality worldwide. While 80% of deaths occur within the first 24 hours after trauma, 20% occur later and are mainly due to healthcare-associated infections, including ventilator-associated pneumonia (VAP). Preventing underinflation of the tracheal cuff is recommended to reduce microaspiration, which plays a major role in the pathogenesis of VAP. Automatic devices facilitate the regulation of tracheal cuff pressure, and their implementation has the potential to reduce VAP...
August 7, 2017: BMJ Open
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