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https://www.readbyqxmd.com/read/29778498/validation-on-milk-and-sprouts-of-en-iso-16654-2001-microbiology-of-food-and-animal-feeding-stuffs-horizontal-method-for-the-detection-of-escherichia-coli-o157
#1
Rosangela Tozzoli, Antonella Maugliani, Valeria Michelacci, Fabio Minelli, Alfredo Caprioli, Stefano Morabito
In 2006, the European Committee for standardisation (CEN)/Technical Committee 275 - Food analysis - Horizontal methods/Working Group 6 - Microbiology of the food chain (TC275/WG6), launched the project of validating the method ISO 16654:2001 for the detection of Escherichia coli O157 in foodstuff by the evaluation of its performance, in terms of sensitivity and specificity, through collaborative studies. Previously, a validation study had been conducted to assess the performance of the Method No 164 developed by the Nordic Committee for Food Analysis (NMKL), which aims at detecting E...
May 8, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29778196/vaccination-with-killed-whole-cells-of-escherichia-coli-o157-h7-hha-mutant-emulsified-with-an-adjuvant-induced-vaccine-strain-specific-serum-antibodies-and-reduced-e-coli-o157-h7-fecal-shedding-in-cattle
#2
Vijay K Sharma, Robert G Schaut, Crystal L Loving
Escherichia coli O157:H7 (O157) can cause from a mild diarrheal illness to hemorrhagic colitis and hemolytic uremic syndrome in humans. Cattle are the primary reservoir for O157 and fecal shedding of O157 by these animals is a major risk factor in contamination of cattle hides and carcasses at slaughter. Vaccination is an important strategy to reduce fecal shedding of O157 in cattle. In this study, we evaluated the immunogenicity and efficacy of an inactivated vaccine strain of O157 formulated with an adjuvant...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29765909/shiga-toxin-producing-escherichia-coli-infection-in-j%C3%A3-nk%C3%A3-ping-county-sweden-occurrence-and-molecular-characteristics-in-correlation-with-clinical-symptoms-and-duration-of-stx-shedding
#3
Xiangning Bai, Sara Mernelius, Cecilia Jernberg, Ing-Marie Einemo, Stefan Monecke, Ralf Ehricht, Sture Löfgren, Andreas Matussek
Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhea (BD), hemorrhagic colitis (HC), and even hemolytic uremic syndrome (HUS). In Nordic countries, STEC are widely spread and usually associated with gastrointestinal symptoms and HUS. The objective of this study was to investigate the occurrence of STEC in Swedish patients over 10 years of age from 2003 through 2015, and to analyze the correlation of critical STEC virulence factors with clinical symptoms and duration of stx shedding. Diarrheal stool samples were screened for presence of stx by real-time PCR...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29765781/bactericidal-and-cytotoxic-activities-of-polyphenol-extracts-from-solanum-tuberosum-spp-tuberosum-and-spp-andigena-cultivars-on-escherichia-coli-and-human-neuroblastoma-sh-sy5y-cells-in-vitro
#4
María Ximena Silveyra, María Luciana Lanteri, Rocío Belén Damiano, Adriana Balbina Andreu
Potatoes ( Solanum tuberosum L.) are a good source of dietary antioxidant polyphenols. This study investigated the potential antioxidant, bactericidal, and cytotoxic activities in vitro of the phenolic compounds present in tubers of one S. tuberosum spp. tuberosum (Summerside), and three S. tuberosum spp. andigena (landraces Moradita, Waicha, and Santa María) cultivars. Both the content of phenolic acids, chlorogenic acids (CGAs) being the most abundant, and the antioxidant activity were higher in extracts from skin than from flesh...
2018: Journal of Nutrition and Metabolism
https://www.readbyqxmd.com/read/29765581/the-antimicrobial-activity-of-probiotic-bacteria-escherichia-coli-isolated-from-different-natural-sources-against-hemorrhagic-e-coli-o157-h7
#5
Sahar Karimi, Fatemeh Azizi, Mohammad Nayeb-Aghaee, Leila Mahmoodnia
Background: Diarrheal diseases have been seen in all geographical areas throughout the world. Therefore, considering treatment, could be deemed a necessary action. Objective: The aim of this study was to determine the antimicrobial effect of probiotic bacterial strains isolated from different natural sources against 2 pathotypes of pathogenic E. coli. Methods: This cross-sectional study of Martyr Chamran University of Ahvaz was carried out from December 2013 to July 2014...
March 2018: Electronic Physician
https://www.readbyqxmd.com/read/29763937/bacteriophage-cocktail-for-biocontrol-of-escherichia-coli-o157-h7-stability-and-potential-allergenicity-study
#6
Karina Ramirez, Carmina Cazarez-Montoya, Hector Samuel Lopez-Moreno, Nohelia Castro-Del Campo
Escherichia coli O157:H7 has become a global public health and a food safety problem. Despite the implementation of control strategies that guarantee the safety in various products, outbreaks persist and new alternatives are necessary to reduce this pathogen along the food chain. Recently, our group isolated and characterised lytic bacteriophages against E. coli O157:H7 with potential to be used as biocontrol agents in food. To this end, phages need certain requirements to allow their manufacture and application...
2018: PloS One
https://www.readbyqxmd.com/read/29763867/shiga-toxigenic-escherichia-coli-incidence-is-related-to-small-area-variation-in-cattle-density-in-a-region-in-ireland
#7
C Brehony, J Cullinan, M Cormican, D Morris
Shiga toxigenic Escherichia coli (STEC) are pathogenic E. coli that cause infectious diarrhoea. In some cases infection may be complicated by renal failure and death. The incidence of human infection with STEC in Ireland is the highest in Europe. The objective of the study was to examine the spatial incidence of human STEC infection in a region of Ireland with significantly higher rates of STEC incidence than the national average and to identify possible risk factors of STEC incidence at area level. Anonymised laboratory records (n = 379) from 2009 to 2015 were obtained from laboratories serving three counties in the West of Ireland...
May 12, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29758485/isolation-characterization-and-strain-selection-of-a-paenibacillus-species-for-use-as-a-probiotic-to-aid-in-ruminal-methane-mitigation-nitrate-nitrite-detoxification-and-food-safety
#8
Elizabeth A Latham, William E Pinchak, Julian Trachsel, Heather K Allen, Todd R Callaway, David J Nisbet, Robin C Anderson
The effects of dietary nitrate and Paenibacillus 79R4 (79R4), a denitrifying bacterium, when co-administered as a probiotic, on methane emissions, nitrate and nitrite-metabolizing capacity and fermentation characteristics were studied in vitro. Mixed populations of rumen microbes inoculated with 79R4 metabolized all levels of nitrite studied after 24 h in vitro incubation. Results from in vitro simulations resulted in up to 2 log10 colony forming unit reductions in E. coli O157:H7 and Campylobacter jejuni when these were co-cultured with 79R4...
April 30, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29758060/genetic-variation-in-humoral-response-to-an-escherichia-coli-o157-h7-vaccine-in-beef-cattle
#9
Kara B Marley, Larry A Kuehn, John W Keele, Benjamin W Wileman, Michael G Gonda
Individuals often respond differently to the same vaccine; some of this variation may be caused by genetic differences among animals. Our objective was to estimate heritability and identify genomic regions associated with humoral response to an Escherichia coli O157:H7 vaccine in beef cattle. Crossbred beef cattle (n = 651) were vaccinated with a commercially available E. coli O157:H7 vaccine. Serum was collected at time of initial vaccination (d 0), booster (d 21), and d 56 after initial vaccination. Total antibodies specific to siderophore receptor and porin proteins in the vaccine were quantified by enzyme-linked immunosorbent assay...
2018: PloS One
https://www.readbyqxmd.com/read/29757011/thermal-inactivation-of-shiga-toxin-producing-escherichia-coli-in-ground-beef-with-varying-fat-content
#10
Jagpinder S Brar, Jolena N Waddell, Matthew Bailey, Sydney Corkran, Carmen Velasquez, Vijay K Juneja, Manpreet Singh
Decimal reduction time ( D-value) was calculated for six non-O157 Shiga toxin-producing Escherichia coli (STEC) in a laboratory medium and ground beef. For the laboratory medium, an overnight culture of each strain of STEC was divided into 10-mL sample bags and heated in a water bath for a specific time on the basis of the temperatures. Survival curves were generated by plotting the surviving bacterial population against time, and a linear-log primary model was used to estimate the D-values from survival curves...
May 14, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29756774/selection-identification-and-binding-mechanism-studies-of-an-ssdna-aptamer-targeted-to-different-stages-of-e-coli-o157-h7
#11
Ying Zou, Nuo Duan, Shijia Wu, Mofei Shen, Zhouping Wang
Enterohemorrhagic Escherichia coli O157:H7 (E.coli O157:H7) is known as an important food-borne pathogens related to public health. In this study, aptamers which could bind to different stages of E.coli O157:H7 (adjustment phase, log phase and stationary phase) with high affinity and specificity was obtained by Whole Cell-SELEX method through fourteen selection rounds including three counter-selection rounds. Altogether, thirty-two sequences were obtained and nine families were classified to select the optimal aptamer...
May 14, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29753990/combination-of-garlic-essential-oil-allyl-isothiocyanate-and-nisin-z-as-bio-preservatives-in-fresh-sausage
#12
Monyque Kais Araújo, Aline Marzaleck Gumiela, Keliani Bordin, Fernando Bittencourt Luciano, Renata Ernlund Freitas de Macedo
The effects of natural antimicrobial compounds (garlic essential oil [GO], allyl isothiocyanate [AITC], and nisin Z [NI]) on microbiological, physicochemical and sensory characteristics of fresh sausage were assessed. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) towards Escherichia coli O157:H7 and Lactobacillus plantarum were determined in vitro. Sausages inoculated with E. coli O157:H7, were treated with different combinations of antimicrobials and assessed for microbiological and physicochemical parameters during storage (6C for 20 d)...
May 3, 2018: Meat Science
https://www.readbyqxmd.com/read/29752274/molecular-epidemiology-of-shiga-toxin-producing-escherichia-coli-stec-on-new-zealand-dairy-farms-application-of-a-culture-independent-assay-and-whole-genome-sequencing
#13
A Springer Browne, Anne C Midwinter, Helen Withers, Adrian L Cookson, Patrick J Biggs, Jonathan C Marshall, Jackie Benschop, Steve Hathaway, Neville A Haack, Rukhshana N Akhter, Nigel P French
New Zealand has a relatively high incidence of human cases of Shiga toxin-producing Escherichia coli (STEC), with 8.9 STEC cases per 100,000 people reported in 2016. Previous research showed living near cattle and contact with cattle feces as significant risk factors for STEC infections in humans in New Zealand, but infection was not linked to food-associated factors. During the 2014 spring calving season, a random stratified cross-sectional study of dairy farms (n=102) in six regions across New Zealand assessed the prevalence of the 'Top 7' STEC (serogroups O157, O26, O45, O103, O111, O121, O145) in young calves (n=1,508) using a culture-independent diagnostic test (PCR/MALDI-TOF)...
May 11, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29748542/probiotic-lactobacillus-fermentum-strain-jdfm216-stimulates-the-longevity-and-immune-response-of-caenorhabditis-elegans-through-a-nuclear-hormone-receptor
#14
Mi Ri Park, Sangdon Ryu, Brighton E Maburutse, Nam Su Oh, Sae Hun Kim, Sejong Oh, Seong-Yeop Jeong, Do-Youn Jeong, Sangnam Oh, Younghoon Kim
Here, we examined the functionality of Lactobacillus fermentum strain JDFM216, a newly isolated probiotic bacterium, using a Caenorhabditis elegans model. We determined bacterial colonization in the intestinal tract of C. elegans by plate counting and transmission electron microscopy and examined the survival of C. elegans using a solid killing assay. In addition, we employed DNA microarray analysis, quantitative real time-polymerase chain reaction, and immunoblotting assays to explore health-promoting pathways induced by probiotic bacteria in C...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29747467/electrochemical-detection-of-e-coli-o157-h7-in-water-after-electrocatalytic-and-ultraviolet-treatments-using-a-polyguanine-labeled-secondary-bead-sensor
#15
Michael G Beeman, Ugochukwu C Nze, Himanshu J Sant, Hammad Malik, Swomitra Mohanty, Bruce K Gale, Krista Carlson
The availability of clean drinking water is a significant problem worldwide. Many technologies exist for purifying drinking water, however, many of these methods require chemicals or use simple methods, such as boiling and filtering, which may or may not be effective in removing waterborne pathogens. Present methods for detecting pathogens in point-of-use (POU) sterilized water are typically time prohibitive or have limited ability differentiating between active and inactive cells. This work describes a rapid electrochemical sensor to differentially detect the presence of active Escherichia coli (E...
May 10, 2018: Sensors
https://www.readbyqxmd.com/read/29745757/inhibition-and-inactivation-of-escherichia-coli-o157-h7-biofilms-by-selenium
#16
Meera Surendran Nair, Abhinav Upadhyay, Samantha Fancher, Indu Upadhyaya, Swayandipta Dey, Anup Kollanoor-Johny, Jing Zhao, Kumar Venkitanarayanan
The present study investigated the efficacy of selenium (Se) in reduction of enterohemorrhagic Escherichia coli (EHEC) exopolysaccharide (EPS) synthesis, inhibition of biofilm formation at 25 and 4°C on polystyrene surface, and inactivation of mature EHEC biofilms in combination with hot water. Sterile 96-well polystyrene plates inoculated with EHEC (∼6.0 log CFU per well) were treated with a subinhibitory concentration (SIC) of Se, and biofilms were allowed to mature at 4 and 25°C for 96 h. Biofilm-associated bacterial population was determined by scraping and plating, whereas the extent of EPS production was determined using ruthenium red staining assay...
May 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29745756/transfer-and-redistribution-of-salmonella-typhimurium-lt2-and-escherichia-coli-o157-h7-during-pilot-scale-processing-of-baby-spinach-cilantro-and-romaine-lettuce
#17
Haley S Smolinski, Siyi Wang, Lin Ren, Yuhuan Chen, Barbara Kowalcyk, Ellen Thomas, Jane VAN Doren, Elliot T Ryser
Several outbreaks of foodborne illness traced to leafy greens and culinary herbs have been hypothesized to involve cross-contamination during washing and processing. This study aimed to assess the redistribution of Salmonella Typhimurium LT2 during pilot-scale production of baby spinach and cilantro and redistribution of Escherichia coli O157:H7 during pilot-scale production of romaine lettuce. Four inoculated surrogate:uninoculated product weight ratios (10:100, 5:100, 1:100, and 0.5:100) and three inoculation levels (103 , 101 , and 10-1 CFU/g) were used for the three commodities...
May 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29745302/shiga-toxin-producing-escherichia-coli-stec-o157-outbreak-associated-with-likely-transmission-in-an-inflatable-home-paddling-pool-in-england-june-2017
#18
Mtr Pereboom, D Todkill, E Knapper, C Jenkins, J Hawker, N Coetzee
In June 2017, an outbreak of Shiga toxin-producing Escherichia coli (STEC) O157 infection with phage type 21/28 and identical genotypic profiles involving three children from Staffordshire was reported. Two cases developed haemolytic uraemic syndrome (HUS). Person-to-person transmission via a shared inflatable home paddling pool was the most likely route of infection, following contamination by the first case. The source of infection in the first case was not identified. We recommend that individuals experiencing gastroenteritis should not bathe in paddling pools and that water should be changed at frequent intervals throughout the day to minimise the spread of infection...
May 1, 2018: Perspectives in Public Health
https://www.readbyqxmd.com/read/29741154/novel-application-of-the-matched-case-control-design-to-compare-food-supply-chains-during-an-escherichia-coli-o157-outbreak-united-kingdom-2016
#19
Thomas Inns, Paul Cleary, Nick Bundle, Sarah Foulkes, Ashley Sharp, Lara Utsi, Chris McBrien, Rehman Teagle, Alison Waldram, Chris Williams, Cathy McCann, Rob Smith, Sepeedeh Saleh, Noel McCarthy, Roberto Vivancos, Jeremy Hawker, Valerie Decraene
There is a need for innovative methods to investigate outbreaks of food-borne infection linked to produce with a complex distribution network. The investigation of a large outbreak of Escherichia coli O157 PT34 infection in the United Kingdom in 2016 indicated that catering venues associated with multiple cases had used salad leaves sourced from one supplier. Our aim was to investigate whether catering venues linked to cases were more likely to have used salad leaves from this supplier. We conducted a matched case-control study, with catering venues as the units of analysis...
May 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29741151/national-outbreak-of-shiga-toxin-producing-escherichia-coli-o157-h7-linked-to-mixed-salad-leaves-united-kingdom-2016
#20
Maya Gobin, Jeremy Hawker, Paul Cleary, Thomas Inns, Daniel Gardiner, Amy Mikhail, Jacquelyn McCormick, Richard Elson, Derren Ready, Tim Dallman, Iain Roddick, Ian Hall, Caroline Willis, Paul Crook, Gauri Godbole, Drazenka Tubin-Delic, Isabel Oliver
We investigated a large outbreak of Escherichia coli O157 in the United Kingdom (UK) with 165 cases between 31 May and 29 July 2016. No linked cases were reported in other countries. Cases were predominately female (n = 128) and adult (n = 150), 66 attended hospital and nine had features of haemorrhagic uraemic syndrome. A series of epidemiological studies (case-control, case-case, ingredients-based and venue-based studies) and supply chain investigations implicated mixed salad leaves from Supplier A as the likely outbreak vehicle...
May 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
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