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https://www.readbyqxmd.com/read/28813002/application-of-surfactant-micelle-entrapped-eugenol-for-prevention-of-growth-of-the-shiga-toxin-producing-escherichia-coli-in-ground-beef
#1
Tamra N Tolen, Songsirin Ruengvisesh, Thomas M Taylor
Beef safety may be compromised by O157 and non-O157 Shiga toxin-producing Escherichia coli (STEC) contamination. The capacity of surfactant micelles loaded with the plant-derived antimicrobial eugenol to reduce STEC on beef trimmings that were later ground and refrigerated for five days at 5 ± 1 °C was tested to determine their utility for beef safety protection. STEC-inoculated trimmings were treated with free eugenol, micelle-encapsulated eugenol, 2% lactic acid (55 °C), sterile distilled water (25 °C), or left untreated (control)...
August 16, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28810248/antibiotic-susceptibility-and-molecular-characterization-of-escherichia-coli-o157-isolates-from-urinary-tract-infections
#2
Zonglan Xie, Ziqiang Dong, Ping Zhu, Lusheng Zhang, Xiaobo Chen, Chuanjiang Dong
BACKGROUND: Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. OBJECTIVES: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E...
August 16, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28807622/community-associated-clostridium-difficile-infection-in-emergency-department-patients-in-western-australia
#3
Deirdre A Collins, Linda A Selvey, Antonio Celenza, Thomas V Riley
Clostridium difficile infection (CDI) is primarily associated with hospitalised patients, however, community-associated CDI (CA-CDI) has increased in Australia. We aimed to investigate the epidemiology and outcomes of CA-CDI cases presenting to hospital emergency departments in Western Australia (WA). A retrospective case-control study of CA-CDI cases presenting at six emergency departments in WA from July 2013 to June 2014 was performed. Clinical signs, recent medication, hospitalisations and potential risk factors for CA-CDI were investigated for cases (n = 34) and unmatched controls (n = 62) who were infected with another gastrointestinal pathogen, including Campylobacter spp...
August 11, 2017: Anaerobe
https://www.readbyqxmd.com/read/28807101/examine-the-correlation-between-heat-shock-protein-ibpa-and-heat-tolerance-in-cronobacter-sakazakii
#4
Zhi Jing Zhao, Bin Wang, Jing Yuan, Hao Yu Liang, Si Guo Dong, Ming Zeng
We used a proteomic approach to identify IbpA in Cronobacter sakazakii (C. sakazaki), which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28805884/the-effects-of-monensin-in-diets-fed-to-finishing-beef-steers-and-heifers-on-growth-performance-and-fecal-shedding-of-escherichia-coli-o157-h7
#5
K E Hales, J E Wells, E D Berry, N Kalchayanand, J L Bono, M Kim
Two experiments were conducted to study the effects of monensin dose on growth performance and O157:H7 shedding in finishing beef cattle. In Exp. 1, 198 heifers (298 ± 1.1 kg BW) were allocated to 1 of 2 treatments consisting of 1) 200 mg/heifer daily of monensin and 2) 400 mg/heifer daily of monensin and fed for 151 d. In Exp. 2, 199 steers (430 ± 1.9 kg BW) were stratified by BW and allocated to 1 of 2 treatments consisting of 1) 0 mg/steer daily of monensin and 2) 400 mg/steer daily of monensin and fed for 128 d...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28805457/selection-of-surrogate-bacteria-for-use-in-food-safety-challenge-studies-a-review
#6
Mengyi Hu, Joshua B Gurtler
Nonpathogenic surrogate bacteria are prevalently used in a variety of food challenge studies in place of foodborne pathogens such as Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Clostridium botulinum because of safety and sanitary concerns. Surrogate bacteria should have growth characteristics and/or inactivation kinetics similar to those of target pathogens under given conditions in challenge studies. It is of great importance to carefully select and validate potential surrogate bacteria when verifying microbial inactivation processes...
August 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28803933/phage-in-urban-wastewater-have-the-potential-to-disseminate-antibiotic-resistance
#7
Gayathri Upeksha Gunathilaka, Varun Tahlan, Abdullah Ibn Mafiz, Manisha Polur, Yifan Zhang
A total of 29 Escherichia coli phage were isolated from wastewater samples collected from an urban wastewater treatment plant (WWTP) and characterized by host range determination, Transmission Electron Microscopy (TEM), antibiotic resistance gene identification, and phage transduction. β-lactam resistance genes, blaCMY, blaTEM, blaSHV, blaCTXM, and blaOXA, were amplified on phage DNA by PCR. Out of nine host range patterns observed, six were able to multiply in three or more indicator strains, including Shiga-toxin producing E...
August 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28803022/short-communication-antioxidative-and-antibacterial-activities-on-staphylococcus-aureus-and-escherichia-coli-o157-h4-in-milk-with-added-ginseng-marc-extract-fermented-by-lactobacillus-plantarum-kccm-11613p
#8
S J Eom, J E Hwang, J Jung, H-S Jee, K-T Kim, H-D Paik
Ginseng marc, a by-product of the extraction of fresh ginseng, is known to have bioactive compounds, but is frequently discarded as agriculture waste. The objectives of our study were to assess the antioxidative activity of fermented ginseng marc extract using Lactobacillus plantarum KCCM 11613P and to evaluate antibacterial activity of fermented milk with added ginseng marc extract during fermentation. After 24 h of fermentation of ginseng marc extract, the viable cell number was increased to 7.7 ± 0.1 log cfu/mL, and the pH and total titratable acidity were 4...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28802271/antibiofilm-effect-of-poly-vinyl-alcohol-co-ethylene-pva-co-pe-halamine-film-against-listeria-innocua-and-escherichia-coli-o157-h7
#9
Andrea Cossu, Yang Si, Gang Sun, Nitin Nitin
Bacterial biofilm formation is linked to several infections and foodborne disease outbreaks. To address this challenge, there is an unmet need to develop rechargeable antimicrobial materials that can provide continuous sanitation of contact surfaces especially in the food industry. This study was aimed at evaluating a novel rechargeable antimicrobial polymer formed using PVA-co-PE with halamine functionality to prevent biofilm formation with repeated exposure to high loads of bacteria and organic content and also aid in inactivation of pre-formed biofilms upon contact with this novel material...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28802105/combined-influence-of-multiple-climatic-factors-on-the-incidence-of-bacterial-foodborne-diseases
#10
Myoung Su Park, Ki Hwan Park, Gyung Jin Bahk
Information regarding the relationship between the incidence of foodborne diseases (FBD) and climatic factors is useful in designing preventive strategies for FBD based on anticipated future climate change. To better predict the effect of climate change on foodborne pathogens, the present study investigated the combined influence of multiple climatic factors on bacterial FBD incidence in South Korea. During 2011-2015, the relationships between 8 climatic factors and the incidences of 13 bacterial FBD, were determined based on inpatient stays, on a monthly basis using the Pearson correlation analyses, multicollinearity tests, principal component analysis (PCA), and the seasonal autoregressive integrated moving average (SARIMA) modeling...
August 9, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28798736/escherichia-coli-o157-h7-acid-sensitivity-correlates-with-flocculation-phenotype-during-nutrient-limitation
#11
Kathryn L Kay, Frederick Breidt, Pina M Fratamico, Gian M Baranzoni, Gwang-Hee Kim, Amy M Grunden, Deog-Hwan Oh
Shiga toxin producing Escherichia coli (STEC) strains vary in acid resistance; however, little is known about the underlying mechanisms that result in strain specific differences. Among 25 STEC O157:H7 strains tested, 7 strains flocculated when grown statically for 18 h in minimal salts medium at 37°C, while 18 strains did not. Interestingly, the flocculation phenotype (cells came out of suspension) was found to correlate with degree of acid sensitivity in an assay with 400 mM acetic acid solution at pH 3...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28797098/comparative-genomics-of-two-super-shedder-isolates-of-escherichia-coli-o157-h7
#12
Robab Katani, Rebecca Cote, Indira T Kudva, Chitrita DebRoy, Terrance M Arthur, Vivek Kapur
Shiga toxin-producing Escherichia coli O157:H7 (O157) are zoonotic foodborne pathogens and of major public health concern that cause considerable intestinal and extra-intestinal illnesses in humans. O157 colonize the recto-anal junction (RAJ) of asymptomatic cattle who shed the bacterium into the environment through fecal matter. A small subset of cattle, termed super-shedders (SS), excrete O157 at a rate (≥ 104 CFU/g of feces) that is several orders of magnitude greater than other colonized cattle and play a major role in the prevalence and transmission of O157...
2017: PloS One
https://www.readbyqxmd.com/read/28794460/host-mechanisms-involved-in-cattle-escherichia-coli-o157-shedding-a-fundamental-understanding-for-reducing-foodborne-pathogen-in-food-animal-production
#13
Ou Wang, Tim A McAllister, Graham Plastow, Kim Stanford, Brent Selinger, Le Luo Guan
The host mechanisms involved in Escherichia coli O157 super-shedding in cattle is largely unknown. In this study, the comparison of transcriptomes of intestinal tissues between super-shedders (SS) and cattle negative for E. coli O157 (NS) was performed, aiming to identify genes that are potentially associated with super-shedding. In total, 16,846 ± 639 (cecum) to 18,137 ± 696 (distal jejunum) were expressed throughout the intestine, with the expressed genes associated with immune functions more pronounced in the small intestine...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28790986/colonisation-of-meat-by-escherichia-coli-o157-h7-investigating-bacterial-tropism-with-respect-to-the-different-types-of-skeletal-muscles-subtypes-of-myofibres-and-postmortem-time
#14
Caroline Chagnot, Annie Venien, Sandra Renier, Nelly Caccia, Régine Talon, Thierry Astruc, Mickaël Desvaux
Escherichia coli O157:H7 is an enterohaemorrhagic E. coli (EHEC) responsible for serious diseases, especially pediatric, and of great concern for the meat industry. Meat contamination by EHEC occurs at slaughtering, especially at dehiding stage, where bacteria can be transferred from hides to carcasses. The skeletal muscle tissues comprise four major types of myofibres, which differ in their contraction velocity and metabolism. Myofibres are surrounded by the extracellular matrix (ECM). Adhesion of E. coli O157:H7 to meat was investigated considering well-defined types of skeletal muscle and their constituent myofibres as well as postmortem changes in muscle, using fluorescence microscopy and immunohistochemical analyses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28774270/strand-specific-transcriptomes-of-enterohemorrhagic-escherichia-coli-in-response-to-interactions-with-ground-beef-microbiota-interactions-between-microorganisms-in-raw-meat
#15
Wessam Galia, Francoise Leriche, Stéphane Cruveiller, Cindy Garnier, Vincent Navratil, Audrey Dubost, Stéphanie Blanquet-Diot, Delphine Thevenot-Sergentet
BACKGROUND: Enterohemorrhagic Escherichia coli (EHEC) are zoonotic agents associated with outbreaks worldwide. Growth of EHEC strains in ground beef could be inhibited by background microbiota that is present initially at levels greater than that of the pathogen E. coli. However, how the microbiota outcompetes the pathogenic bacteria is unknown. Our objective was to identify metabolic pathways of EHEC that were altered by natural microbiota in order to improve our understanding of the mechanisms controlling the growth and survival of EHECs in ground beef...
August 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28768110/first-report-of-human-gastroenteritis-caused-by-escherichia-coli-o157-nm-in-brazil
#16
Renata França Castro Santos, Janaína Dos Santos Nascimento, Mercedes Passos Geimba, Claudia Titze Hessel, Eduardo Cesar Tondo
In September 2005, the Sanitary Surveillance Service of Rio de Janeiro (SSS/RJ), Brazil, investigated a case of gastroenteritis involving a 13-year-old teenager hospitalized because of bloody diarrhea and severe abdominal pain. Owing to the severity of the symptoms, an epidemiological investigation was conducted in two states of Brazil. Escherichia coli O157:NM was isolated from stools and from a tomato and cheese salad prepared at the school canteen where the teenager attended. This is the first report of a human case of gastroenteritis related to E...
August 2, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28755469/a-randomized-controlled-trial-to-evaluate-the-effects-of-dietary-fibre-from-distillers-grains-on-enterohemorrhagic-escherichia-coli-detection-from-the-rectoanal-mucosa-and-hides-of-feedlot-steers
#17
L G Schneider, T J Klopfenstein, Z R Stromberg, G L Lewis, G E Erickson, R A Moxley, D R Smith
Feeding high levels (≥40% dry matter) of distillers grains may increase the risk for cattle to carry enterohemorrhagic Escherichia coli (EHEC) O157. The mechanism for the increased risk is not known nor whether non-O157 EHEC are similarly affected. Our objective was to test whether the fibre content or other components of modified distillers grains plus solubles (MDGS) affects the probability for cattle to carry EHEC serogroups of public health importance. A 2 × 2 plus 1 factorial treatment arrangement within a randomized block design was utilized...
July 28, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28753730/a-microfluidic-device-for-rapid-screening-of-e-coli-o157-h7-based-on-ifast-and-atp-bioluminescence-assay-for-water-analysis
#18
Bongkot Ngamsom, Alma Truyts, Louis Fourie, Shavon Kumar, Mark D Tarn, Alexander Iles, Klariska Moodley, Kevin J Land, Nicole Pamme
We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from 6 colony forming units (CFU) in 1 mL spiked buffer within 20 minutes. In tests with wastewater discharged effluent samples, without pre-concentration, the device could detect 104 seeded CFU per mL; suggesting great potential for point-of-need microbiological water quality monitoring...
July 28, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28753655/bacterial-effector-nlel-promotes-enterohemorrhagic-e-coli-induced-attaching-and-effacing-lesions-by-ubiquitylating-and-inactivating-jnk
#19
Xiangpeng Sheng, Qing You, Hongnian Zhu, ZeNan Chang, Qingrun Li, Haifeng Wang, Chen Wang, Hongyan Wang, Lijian Hui, Chongtao Du, Xiaoduo Xie, Rong Zeng, Anning Lin, Dongfang Shi, Kangcheng Ruan, Jinghua Yan, George Fu Gao, Feng Shao, Ronggui Hu
As a major diarrheagenic human pathogen, enterohemorrhagic Escherichia coli (EHEC) produce attaching and effacing (A/E) lesions, characterized by the formation of actin pedestals, on mammalian cells. A bacterial T3SS effector NleL from EHEC O157:H7 was recently shown to be a HECT-like E3 ligase in vitro, but its biological functions and host targets remain elusive. Here, we report that NleL is required to effectively promote EHEC-induced A/E lesions and bacterial infection. Furthermore, human c-Jun NH2-terminal kinases (JNKs) were identified as primary substrates of NleL...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28753059/growth-of-salmonella-spp-and-escherichia-coli-o157-h7-on-fresh-cut-fruits-stored-at-different-temperatures
#20
Ke Feng, Wenzhong Hu, Aili Jiang, Gaowa Saren, Yongping Xu, Yaru Ji, Wenjun Shao
The objective of this work was to determine the growth potential of Salmonella spp. and Escherichia coli O157:H7 on fresh-cut honeydew melon, cantaloupe, watermelon, pitaya, mango, papaya, and pineapple stored at 5°C, 13°C, and 25°C. The results showed that both pathogens were able to grow on fresh-cut fruits except fresh-cut pineapple at 13°C and 25°C. Salmonella spp. grew more rapidly on fresh-cut honeydew melon, cantaloupe, watermelon, and mango than did E. coli O157:H7 at 13°C. The growth of both species was inhibited on fresh-cut pineapple, with that of Salmonella spp...
July 28, 2017: Foodborne Pathogens and Disease
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