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https://www.readbyqxmd.com/read/28735241/a-new-integrated-approach-to-analyze-bulk-tank-milk-and-raw-milk-filters-for-the-presence-of-the-e-coli-serogroups-frequently-associated-with-vtec-status
#1
Francesca Albonico, Claudia Gusmara, Theo Gugliotta, Monica Loiacono, Michele Mortarino, Alfonso Zecconi
We optimized a combination of microbiological and molecular methods to quickly identify the presence of the O157 and the six non-O157 serogroups (O26, O45, O103, O111, O121 and O145) most frequently associated with VTEC status, at herd level. The lower detection limit of this methodology is 10(1)CFU/ml for each of the serogroups tested. We tested 67 bulk tank milk (BTM) and raw milk filters (RMF) derived from dairy herds located in Lombardy and Trentino Alto Adige. We identified 3 positive samples and 20 positive samples out of 67 respectively in the BTM and RMF...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28732694/development-and-evaluation-of-latex-agglutination-tests-for-the-detection-of-human-antibodies-to-the-lipopolysaccharides-of-verocytotoxin-producing-escherichia-coli-vtec-serogroups-o157-and-non-o157
#2
Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec
Latex agglutination tests (LAT) were developed and evaluated for the rapid detection of LPS antibodies against E. coli serogroup O157, O26, O104, O111 and O145. The latex tests have been proved to be sensitive, fast, easy-to-perform and cost-efficient tools for the screening serodiagnosis of VTEC infections causing haemolytic-uraemic syndrome.
July 18, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28728309/escherichia-coli-outer-membrane-protein-f-ompf-an-immunogenic-protein-induces-cross-reactive-antibodies-against-escherichia-coli-and-shigella
#3
Xiao Wang, Da Teng, Qingfeng Guan, Ruoyu Mao, Ya Hao, Xiumin Wang, Junhu Yao, Jianhua Wang
Diarrhea caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases in humans and animals. Due to antibiotics resistance and the lack of efficient vaccine, more attention should be paid to find potential versatile vaccine candidates to prevent diseases. In this study, the sequence homology analysis indicated that OmpF from E. coli CVCC 1515 shares a high identity (90-100%) with about half of the E. coli (46.7%) and Shigella (52.8%) strains. Then the recombinant OmpF was supposed to be developed as a versatile vaccine to prevent E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28726627/mucus-activatable-shiga-toxin-genotype-stx2d-in-escherichia-coli-o157-h7
#4
Sergio Sánchez, María Teresa Llorente, Laura Herrera-León, Raquel Ramiro, Sandra Nebreda, María Antonia Remacha, Silvia Herrera-León
We identified the mucus-activatable Shiga toxin genotype stx2d in the most common hemolytic uremic syndrome-associated Escherichia coli serotype, O157:H7. stx2d was detected in a strain isolated from a 2-year-old boy with bloody diarrhea in Spain, and whole-genome sequencing was used to confirm and fully characterize the strain.
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28726488/use-of-an-electrostatic-spraying-system-or-the-sprayed-lethality-in-container-method-to-deliver-antimicrobial-agents-onto-the-surface-of-beef-subprimals-to-control-shiga-toxin-producing-escherichia-coli
#5
J Max Stella, John B Luchansky, Kelsey Miller, Bradley A Shoyer, Laura E Shane, Lianna McGeary, Manuela Osoria, Laura J Stahler, Nicholas J Sevart, Randall K Phebus, Harshavardhan Thippareddi, Anna C S Porto-Fett
The efficacy of an electrostatic spraying system (ESS) and/or the sprayed lethality in container (SLIC) method to deliver antimicrobial agents onto the surface of beef subprimals to reduce levels of Shiga toxin-producing Escherichia coli (STEC) was evaluated. Beef subprimals were surface inoculated (lean side; ca. 5.8 log CFU per subprimal) with 2 mL of an eight-strain cocktail comprising single strains of rifampin-resistant (100 μg/mL) STEC (O26:H11, O45:H2, O103:H2, O104:H4, O111:H(-), O121:H19, O145:NM, and O157:H7)...
July 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28723213/dual-functional-core-shell-fluorescent-ag2s-carbon-nanostructure-for-selective-assay-of-e-coli-o157-h7-and-bactericidal-treatment
#6
Ning Wang, Xing Wei, An-Qi Zheng, Ting Yang, Ming-Li Chen, Jian-Hua Wang
A dual functional fluorescent core-shell Ag2S@Carbon nanostructure is prepared by a hydrothermally assisted multi-amino synthesis approach with folic acid (FA), polyethylenimine (PEI), and mannoses (Mans) as carbon and nitrogen sources (FA-PEI-Mans-Ag2S nanocomposite shortly as Ag2S@C). The nanostructure exhibits strong fluorescent emission at λex/λem = 340/450 nm with a quantum yield of 12.57 ± 0.52%. Ag2S@C is bound to E. coli O157:H7 via strong interaction with the Mans moiety in Ag2S@C with FimH proteins on the fimbriae tip in E...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28714572/surface-modified-mesh-filter-for-direct-nucleic-acid-extraction-and-its-application-to-gene-expression-analysis
#7
Jae Bem You, Yong Tae Kim, Kyoung G Lee, Yunho Choi, Seongkyun Choi, Chi Hyun Kim, Kyung Hoon Kim, Sung Jin Chang, Tae Jae Lee, Seok Jae Lee, Sung Gap Im
Rapid and convenient isolation of nucleic acids (NAs) from cell lysate plays a key role for onsite gene expression analysis. Here, this study achieves one-step and efficient capture of NA directly from cell lysate by developing a cationic surface-modified mesh filter (SMF). By depositing cationic polymer via vapor-phase deposition process, strong charge interaction is introduced on the surface of the SMF to capture the negatively charged NAs. The NA capturing capability of SMF is confirmed by X-ray photoelectron spectroscopy, fluorescent microscopy, and zeta potential measurement...
July 17, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28713792/shiga-toxin-producing-escherichia-coii-in-slaughtered-pigs-and-pork-products
#8
Lia Bardasi, Roberta Taddei, Ilaria Fiocchi, Maria Francesca Pelliconi, Mattia Ramini, Elena Toschi, Giuseppe Merialdi
During the years 2015-2016, 83 faecal samples were collected at slaughter from pigs reared in farms located in Central-Northern Italy. During the years 2014-2016 a total of 562 pork products [465 not-readyto-eat (NRTE) and 97 ready-to-eat (RTE) products] were collected from retail outlets, large retailers and processing plants. The samples were analysed according to ISO TS 13136:2012. Out of 83 swine faecal samples, 77 (92.8%) resulted stx-positive by real time polymerase chain reaction (PCR), 5 stx2+ and 1 stx1+ Shiga toxin-producing Escherichia coli (STEC) strains were isolated...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28711240/characterization-and-bioactivities-of-the-exopolysaccharide-from-a-probiotic-strain-of-lactobacillus-plantarum-wlpl04
#9
Zhengqi Liu, Zhihong Zhang, Liang Qiu, Fen Zhang, Xiongpeng Xu, Hua Wei, Xueying Tao
Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10(4) Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28708031/persistence-of-escherichia-coli-o157-h7-and-total-escherichia-coli-in-feces-and-feedlot-surface-manure-from-cattle-fed-diets-with-and-without-corn-or-sorghum-wet-distillers-grains-with-solubles
#10
Elaine D Berry, James E Wells, Vincent H Varel, Kristin E Hales, Norasak Kalchayanand
Feeding corn wet distillers grains with solubles (WDGS) to cattle can increase the load of Escherichia coli O157:H7 in feces and on hides, but the mechanisms are not fully understood. The objective of these experiments was to examine a role for the persistence of E. coli O157:H7 in the feces and feedlot pen surfaces of cattle fed WDGS. In the first study, feces from steers fed 0, 20, 40, or 60% corn WDGS were inoculated with E. coli O157:H7. The E. coli O157:H7 numbers in feces from cattle fed 0% corn WDGS rapidly decreased (P < 0...
July 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28693954/nano-encapsulation-of-chicken-immunoglobulin-igy-in-sodium-alginate-nanoparticles-in%C3%A2-vitro-characterization
#11
Mostafa Bakhshi, Firouz Ebrahimi, Shahram Nazarian, Jamil Zargan, Faeze Behzadi, Dawood Sadeghi Gariz
Controlled delivery of therapeutic agents by alginate nanoparticles became an attractive issue in the gastric organ. Some therapeutic agents such as proteins could not tolerate in severe condition in the gastrointestinal tract. In the present study, four concentrations of a specific IgY as a prophylactic agent against E. coli O157: H7 was entrapped in 0.2% w/v sodium alginate nanoparticles by ionic gelation method. Depending on the IgY concentration entrapment efficacy was 28.31-99.84%. The physicochemical and structural characteristics of free and IgY-loaded Alg NPs revealed that the individual particles exhibited a spherical shape with a diameter of 45-85 nm, and a negatively charged surface with a zeta potential value of 26-36 mV...
July 7, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28675870/urea-derived-graphitic-carbon-nitride-u-g-c3n4-films-with-highly-enhanced-antimicrobial-and-sporicidal-activity
#12
John H Thurston, Necia M Hunter, Lacey J Wayment, Kenneth A Cornell
In this manuscript, we describe the fabrication of photoactive biocidal or sporicidal films from urea-derived graphitic carbon nitride (u-g-C3N4). Co-deposited films of u-g-C3N4 and Escherichia coli O157:H7 (IC50=14.1±0.2mJ) or Staphylococcus aureus (methicillin resistant IC50=33.5±0.2mJ, methicillin sensitive IC50=42.7±0.5mJ) demonstrated significantly enhanced bactericidal behavior upon administration of visible radiation (400nm≤λ≤426nm). In all cases, complete eradication of the microbial sample was realized upon administration of 100mJ of visible radiation, while no antimicrobial activity was observed for non-irradiated samples...
June 27, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28675605/intrahost-milieu-modulates-production-of-outer-membrane-vesicles-vesicle-associated-shiga-toxin-2a-and-cytotoxicity-in-escherichia-coli-o157-h7-and-o104-h4
#13
Andreas Bauwens, Lisa Kunsmann, Monika Marejková, Wenlan Zhang, Helge Karch, Martina Bielaszewska, Alexander Mellmann
Outer membrane vesicles (OMVs) are important virulence tools of enterohaemorrhagic Escherichia coli (EHEC), but other biological functions of these nanostructures are unknown. We tested the hypothesis that modulation of OMV production enables EHEC to resist the intrahost environment during infection by investigating if simulated human gastrointestinal conditions affect OMV production in EHEC O157:H7 and O104:H4. All the conditions tested including a low pH, simulated ileal and colonic media, presence of mucin, intestinal epithelial cell lysate or antimicrobial peptides, as well as iron limitation, significantly increased OMV production by these pathogens...
July 4, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28674066/contribution-of-the-chromosomal-ccdab-operon-to-bacterial-drug-tolerance
#14
Kritika Gupta, Arti Tripathi, Alishan Sahu, Raghavan Varadarajan
One of the first identified and best studied toxin-antitoxin systems in Escherichia coli is the F-plasmid based CcdAB system. This system is involved in plasmid maintenance through post-segregational killing. More recently, ccdAB homologs have been found on the chromosome, including in pathogenic strains of E. coli and other bacteria. However the functional role of chromosomal ccdAB genes, if any, has remained unclear. We show that both the native ccd operon of the E.coliO157 strain, as well as the ccd operon from the F- plasmid, when inserted on the E...
July 3, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28672173/prevalence-of-verocytotoxigenic-escherichia-coli-strains-isolated-from-raw-beef-in-southern-italy
#15
Gaia Nobili, Ilenia Franconieri, Gianfranco La Bella, Maria Grazia Basanisi, Giovanna La Salandra
Verocytotoxin (VT)-producing Escherichia coli (VTEC) are a significant foodborne public health hazard, where most human infections are associated with six serogroups (O157, O26, O103, O145, O111 and O104). VTEC was the fourth most commonly reported zoonosis in the EU in 2015, with 5901 confirmed human cases. Ruminant animals, including cattle, are a major reservoir of VTEC. The consumption of VTEC-contaminated animal-derived foodstuffs, especially undercooked ground beef, is an important transmission route...
June 28, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28668727/inactivation-of-viruses-and-bacteria-on-strawberries-using-a-levulinic-acid-plus-sodium-dodecyl-sulfate-based-sanitizer-taking-sensorial-and-chemical-food-safety-aspects-into-account
#16
Zijin Zhou, Sophie Zuber, Frédérique Cantergiani, Sophie Butot, Dan Li, Thomas Stroheker, Frank Devlieghere, Anthony Lima, Umberto Piantini, Mieke Uyttendaele
The efficacy of levulinic acid (LVA) in combination with sodium dodecyl sulfate (SDS) in removal of foodborne viruses, enteric bacterial pathogens and their surrogates on fresh strawberries was investigated. Inoculated strawberries were treated with potable water, sodium hypochlorite solution (50ppm), 0.5% LVA plus 0.5% SDS solution, and 5% LVA plus 2% SDS solution respectively for 2min, followed by spray-rinsing with potable water. Water washing removed at least 1.0-log of the tested viral and bacterial strains from the strawberries' surfaces...
June 23, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28663823/sequencing-a-piece-of-history-complete-genome-sequence-of-the-original-escherichia-coli-strain
#17
Karl A Dunne, Roy R Chaudhuri, Amanda E Rossiter, Irene Beriotto, Douglas F Browning, Derrick Squire, Adam F Cunningham, Jeffrey A Cole, Nicholas Loman, Ian R Henderson
In 1885, Theodor Escherich first described the Bacillus coli commune, which was subsequently renamed Escherichia coli. We report the complete genome sequence of this original strain (NCTC 86). The 5 144 392 bp circular chromosome encodes the genes for 4805 proteins, which include antigens, virulence factors, antimicrobial-resistance factors and secretion systems, of a commensal organism from the pre-antibiotic era. It is located in the E. coli A subgroup and is closely related to E. coli K-12 MG1655. E...
March 2017: Microbial Genomics
https://www.readbyqxmd.com/read/28659895/autoinducer-2-quorum-sensing-influences-viability-of-escherichia-coli-o157-h7-under-osmotic-and-in-vitro-gastrointestinal-stress-conditions
#18
Hyunjoon Park, Kyuyeon Lee, Soyoung Yeo, Heuynkil Shin, Wilhelm H Holzapfel
Bacteria use autoinducer molecules to communicate both at intra-species and inter-species levels by quorum sensing. One such cell density-dependent signaling system is the luxS-mediated universal quorum sensing using autoinducer-2 (AI-2). Virulence of several pathogens is determined by an AI-2 system and is related to colonization and infection of the host. From this concept, numerous papers have suggested that AI-2 inhibition is an important strategy toward designing of new antimicrobial agents. However, recent studies indicate that the AI-2 system is also involved in adaptation and survival under environmental stress conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28656639/comparison-of-immunomagnetic-separation-beads-for-detection-of-six-non-o157-shiga-toxin-producing-escherichia-coli-serogroups-in-different-matrices
#19
Autumn L Kraft, David W Lacher, Weilin L Shelver, Julie S Sherwood, Teresa M Bergholz
Immunomagnetic separation used with culture based methods has been a useful technique in the detection of pathogens. However, previous studies have not answered many of the necessary questions for real world applications. The objective of this study was to assess the efficacy of different IMS bead types in recovery of the correct serogroup from a mixture of big six non-O157 STEC strains. To determine the impact of different matrices on recovery, samples of sterile phosphate buffered saline (PBS), sterile and non-sterile cattle feces, ground beef, and lettuce were inoculated with 10 CFU ml(-1) mixture of isolates representing the six serogroups...
June 28, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28655830/correction-for-luzader-et-al-eutr-is-a-direct-regulator-of-genes-that-contribute-to-metabolism-and-virulence-in-enterohemorrhagic-escherichia-coli-o157-h7
#20
Deborah H Luzader, David E Clark, Laura A Gonyar, Melissa M Kendall
No abstract text is available yet for this article.
July 15, 2017: Journal of Bacteriology
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