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https://www.readbyqxmd.com/read/28550057/prevalence-and-genomic-characterization-of-escherichia-coli-o157-h7-in-cow-calf-herds-throughout-california
#1
Jay N Worley, Kristopher A Flores, Xun Yang, Jennifer A Chase, Guojie Cao, Shuai Tang, Jianghong Meng, Edward R Atwill
Escherichia coli serotype O157:H7 (E. coli O157:H7) is a zoonotic food- and waterborne bacterial pathogen that has a high hospitalization rate and can cause life-threatening complications. Increasingly, E. coli O157:H7 infections appear to originate from fresh produce. Ruminants, like cattle, are a prominent reservoir of E. coli O157:H7 in the United States. California is one of the most agriculturally productive regions in the world for fresh produce, beef, and milk. The close proximity of fresh produce and cattle present food safety challenges on a uniquely large scale...
May 26, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28544962/effect-of-immobilization-on-the-antimicrobial-activity-of-a-cysteine-terminated-antimicrobial-peptide-cecropin-p1-tethered-to-silica-nanoparticle-against-e-coli-o157-h7-edl933
#2
Xi Wu, Pei-Hsun Wei, Xiao Zhu, Mary J Wirth, Arun Bhunia, Ganesan Narsimhan
Antimicrobial peptides (AMPs) have the ability to penetrate the cell membrane, form pores which eventually lead to cell death. Immobilization of AMP on nanoparticles can play a major role in antimicrobial materials, biosensors for pathogen detection and in food safety. The minimum inhibitory concentration (MIC) of free Cecropin P1 (CP1, sequence SWLSTAKKLENSAKKRLSEGIAIAIQGGPR) and adsorbed on silica nanoparticle against E. coli O157:H7 EDL933 were 0.78μg/ml. This was found to be consistent with preservation of α-helical secondary structure of CP1 upon adsorption as indicated by circular dichroism (CD)...
May 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28543603/silver-nanoparticles-antimicrobial-activity-cytotoxicity-and-synergism-with-n-acetyl-cysteine
#3
Selwan Hamed, Mohamed Emara, Riham M Shawky, Ramadan A El-Domany, Tareq Youssef
The fast progression of nanotechnology has led to novel therapeutic interventions. Antimicrobial activities of silver nanoparticles (Ag NPs) were tested against standard ATCC strains of Staphylococcus aureus (ATCC 9144), Escherichia coli (O157:H7), Pseudomonas aeruginosa (ATCC 27853), and Candida albicans (ATCC 90028) in addition to 60 clinical isolates collected from cancer patients. Antimicrobial activity was tested by disk diffusion method and MIC values for Ag NPs alone and in combination with N-acetylcysteine (NAC) against tested pathogens were determined by broth microdilution method...
May 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28543177/fingerprints-of-resistant-escherichia-coli-o157-h7-from-vegetables-and-environmental-samples
#4
G O Abakpa, V J Umoh, S Kamaruzaman, A M Ibekwe
BACKGROUND: Some routes of transmission of E. coli O157:H7 to fresh produce include contaminated irrigation water and manure polluted soils. The aim of this study was to determine the genetic relationships of E. coli O157:H7 isolated from some produce growing region in Nigeria using Enterorepititive Intergenic Consensus Deoxyribonucleic Acid Fingerprinting (ERIC-PCR) analysis. A total of four hundred and forty samples comprising leafy greens, irrigation water, manure, and soil were obtained from vegetable producing regions in Kano and Plateau States, Nigeria...
May 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28542913/reduction-of-escherichia-coli-o157-h7-in-biofilms-using-bacteriophage-bpeco-19
#5
Mohammad Sadekuzzaman, Sungdae Yang, Md Furkanur Rahaman Mizan, Sang-Do Ha
Biofilm formation is a growing concern in the food industry. Escherichia coli O157:H7 is one of the most important foodborne pathogens that can persists in food and food-related environments and subsequently produce biofilms. The efficacy of bacteriophage BPECO 19 was evaluated against three E. coli O157:H7 strains in biofilms. Biofilms of the three E. coli O157:H7 strains were grown on abiotic (stainless steel, rubber, and minimum biofilm eradication concentration [MBEC(TM) ] device) and biotic (lettuce) surfaces at different temperatures...
May 19, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28536016/production-and-characterization-of-a-new-biodegradable-fenugreek-seed-gum-based-active-nanocomposite-film-reinforced-with-nanoclays
#6
Saliha Memiş, Fatih Tornuk, Fatih Bozkurt, M Zeki Durak
In the present work, fenugreek seed gum (FSG)/clay nanocomposite films were prepared with nanoclays (Na(+) montmorillonite [MMT], halloysite [HNT] and Nanomer® I.44 P [NM]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g FSG) by solution casting method and characterized. Increasing amount of nanoclay significantly (P<0.05) improved oxygen barrier and thermal properties of the biodegradable films. Agar diffusion tests revealed that FSG based nanocomposite films exhibited strong antimicrobial properties against foodborne pathogens namely Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus and Bacillus cereus independently of clay type and concentration...
May 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527243/-synthesis-of-antibiotic-loaded-polylactic-acid-nanoparticles-and-their-antibacterial-activity-against-escherichia-coli-o157-h7-and-methicillin-resistant-staphylococcus-aureus
#7
Mónica Tatiana Herrera, Jhon Jhamilton Artunduaga, Claudia Cristina Ortiz, Rodrigo Gonzalo Torres
INTRODUCTION: Polymeric nanoparticles are promising nanotechnology tools to fight pathogenic bacteria resistant to conventional antibiotics. OBJECTIVE: To synthesize polylactic acid nanoparticles loaded with ofloxacin and vancomycin, and to determine their antibacterial activity against Escherichia coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA). MATERIALS AND METHODS: We synthesized ofloxacin or vancomycin loaded polylactic acid nanoparticles by the emulsification-solvent evaporation method, and characterized them by dynamic light scattering, laser Doppler electrophoresis and scanning electron microscopy...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28525985/investigation-of-optimum-ohmic-heating-conditions-for-inactivation-of-escherichia-coli-o157-h7-salmonella-enterica-serovar-typhimurium-and-listeria-monocytogenes-in-apple-juice
#8
Il-Kyu Park, Jae-Won Ha, Dong-Hyun Kang
BACKGROUND: Control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. However, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. This study aims to investigate effective voltage gradients of an ohmic heating system and corresponding sugar concentrations (°Brix) of apple juice for inactivating major foodborne pathogens (E...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28515721/efficacy-of-lariciresinol-to-control-bacterial-growth-of-staphylococcus-aureus-and-escherichia-coli-o157-h7
#9
Vivek K Bajpai, Shruti Shukla, Woon K Paek, Jeongheui Lim, Pradeep Kumar, Pankaj Kumar, MinKyun Na
This study was undertaken to assess the antibacterial potential of a polyphenolic compound (+)-lariciresinol isolated from Rubia philippinensis against selected foodborne pathogens Staphylococcus aureus KCTC1621 and Escherichia coli O157:H7. (+)-Lariciresinol at the tested concentrations (250 μg/disk) evoked a significant antibacterial effect as a diameter of inhibition zones (12.1-14.9 mm) with minimum inhibitory concentration (MIC), and minimum bactericidal concentration values of 125-250 and 125-250 μg/mL, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28511109/sensitive-detection-of-viable-escherichia-coli-o157-h7-from-foods-using-a-luciferase-reporter-phage-phiv10lux
#10
Jinwoo Kim, Minsik Kim, Seongmi Kim, Sangryeol Ryu
Escherichia coli O157:H7, a major foodborne pathogen, is a major public health concern associated with life-threatening diseases such as hemolytic uremic syndrome. To alleviate this burden, a sensitive and rapid system is required to detect this pathogen in various kinds of foods. Herein, we propose a phage-based pathogen detection method to replace laborious and time-consuming conventional methods. We engineered an E. coli O157:H7-specific phage phiV10 to rapidly and sensitively detect this notorious pathogen...
May 5, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28508923/biomarkers-of-oxidative-damage-in-bacteria-for-the-assessment-of-sanitation-efficacy-in-lettuce-wash-water
#11
Andrea Cossu, Fang Dou, Glenn M Young, Nitin Nitin
In the fresh produce industry, validation of sanitation efficacy is critical to prevent cross-contamination of produce. The current validation approaches are either based on time-consuming plate counting assays or indirect measurements of chemical properties of wash water. In the study, the focus was to identify biomarkers that can provide direct assessment of oxidative damage in bacteria upon exposure to sanitizers in the presence of fresh produce and correlation of these oxidative biomarkers with logarithmic inactivation of bacteria...
May 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28506793/frequency-and-risk-factors-analysis-of-escherichia-coli-o157-h7-in-bali-cattle
#12
I Wayan Suardana, Dyah Ayu Widiasih, Widagdo Sri Nugroho, Michael Haryadi Wibowo, I Nyoman Suyasa
Cattle are known as the main reservoir of zoonotic agents verocytotoxin-producing Escherichia coli. These bacteria are usually isolated from calves with diarrhea and/or mucus and blood. Tolerance of these agents to the environmental conditions will strengthen of their transmission among livestock. A total of 238 cattle fecal samples from four sub-districts in Badung, Bali were used in this study. Epidemiological data observed include cattle age, sex, cattle rearing system, the source of drinking water, weather, altitude, and type of cage floor, the cleanliness of cage floor, the slope of cage floor, and the level of cattle cleanliness...
May 12, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28505584/development-of-a-gold-nanoparticle-based-universal-oligonucleotide-microarray-for-multiplex-and-low-cost-detection-of-foodborne-pathogens
#13
Xiaoqiang Wang, Sisi Ying, Xiaoguang Wei, Jun Yuan
Bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. In this study a novel assay, combining gold nanoparticle (GNP)-based multiplex oligonucleotide ligation-PCR and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including Shigella spp., Campylobacter jejuni, Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, Staphylococcus aureus, and Vibrio parahaemolyticus...
May 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28499121/molecular-characterization-of-o157-h7-o26-h11-and-o103-h2-shiga-toxin-producing-escherichia-coli-isolated-from-dairy-products
#14
T Douëllou, S Delannoy, S Ganet, P Fach, E Loukiadis, M-C Montel, D Sergentet-Thevenot
Pathogenic Shiga toxin-producing E. coli (STEC) are recognized worldwide as environment and foodborne pathogens which can be transmitted by ingestion of ready-to-eat food such as raw milk-derived products. STEC show a prevalence rate in dairy products of 0.9%, yet comparably few outbreaks have been related to dairy products consumption. In this study, we used rt-qPCR to identify the virulence potential of O157, O26 and O103 STEC strains isolated from raw-milk dairy products by analyzing virulence-related gene frequencies and associations with O-island (OI) 44, OI-48, OI-50, OI-57, OI-71 and OI-122...
April 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28493685/selective-detection-of-shiga-like-toxin-1-from-complex-samples-using-pigeon-ovalbumin-functionalized-gold-nanoparticles-as-affinity-probes
#15
Chun-Hsien Li, Yi-Ling Bai, Karuppuchamy Selvaprakash, Kwok-Kong Tony Mong, Yu-Chie Chen
Escherichia coli O157:H7 is a foodborne pathogen. This bacterial strain can generate Shiga-like toxins (SLTs), which can cause serious sickness and even death. Thus, it is important to develop effective and sensitive methods that can be used to rapidly identify the presence of SLTs from complex samples. Pigeon egg white (PEW) contains abundant glycoproteins, including pigeon ovalbumin (POA) (∼60%). POA possesses Gal-α(1→4)-Gal-β(1→4)-GlcNAc termini, which can recognize the B subunits in SLT type 1 (SLT-1B)...
May 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28485631/comparative-effect-of-heat-shock-on-survival-of-o157-h7-and-non-o157-shiga-toxigenic-escherichia-coli-and-salmonella-in-lean-beef-with-or-without-moisture-enhancing-ingredients
#16
Akhila Vasan, Steven C Ingham, Barbara H Ingham
Thermal tolerance of pathogenic bacteria has been shown to increase after exposure to sublethal elevated temperatures, or heat shock. We evaluated the effect of heat shock at 48°C on thermal tolerance (D55°C) of cocktails of O157 and non-O157 Shiga toxigenic Escherichia coli (STEC) and Salmonella in lean ground beef with or without moisture-enhancing ingredients. Beef was moisture enhanced to 110% (w) with a 5% NaCl-2.5% sodium tripolyphosphate (w/w) brine. Meat, with or without added brine, was inoculated (∼10(8) CFU/g) and heat shocked at 48°C for 0, 5, or 30 min, followed by isothermal heating at 55°C...
May 9, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28484684/the-nag-sensor-nagc-regulates-lee-gene-expression-and-contributes-to-gut-colonization-by-escherichia-coli-o157-h7
#17
Guillaume Le Bihan, Jean-Félix Sicard, Philippe Garneau, Annick Bernalier-Donadille, Alain P Gobert, Annie Garrivier, Christine Martin, Anthony G Hay, Francis Beaudry, Josée Harel, Grégory Jubelin
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 are human pathogens responsible for bloody diarrhea and renal failures. EHEC employ a type 3 secretion system to attach directly to the human colonic epithelium. This structure is encoded by the locus of enterocyte effacement (LEE) whose expression is regulated in response to specific nutrients. In this study, we show that the mucin-derived sugars N-acetylglucosamine (NAG) and N-acetylneuraminic acid (NANA) inhibit EHEC adhesion to epithelial cells through down-regulation of LEE expression...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28476502/quercetin-suppresses-nlrp3-inflammasome-activation-in-epithelial-cells-triggered-by-escherichia-coli-o157-h7
#18
Yansong Xue, Min Du, Mei-Jun Zhu
Inflammatory responses elicited by LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is induced by a wide variety of stress signals including infectious agents and cellular disorders. E. coli O157:H7 causes serious gastrointestinal diseases that results in severe inflammation and oxidative stress, causing host cell damage. In this study, we found that E. coli O157:H7 infection induced NLRP3 assembly, caspase-1 activation and interleukin (IL)-1β and IL-18 release in Caco-2 cells. Infection also resulted in mitochondrial dysfunction with disrupted mitochondrial potential and mitochondrial complex-I activity, as well as the cytosolic release of cytochrome c and altered mitochondrial respiratory chain...
May 2, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28474971/effect-of-ultrasound-on-some-chemical-and-microbiological-properties-of-sour-cherry-juice-by-response-surface-methodology
#19
Tuğba Türken, Hande S Erge
In this study, it is aimed to determine effect of ultrasonication on some chemical and microbiological properties of sour cherry juice by response surface methodology, since ultrasound is known as an alternative method for thermal food processing. Sour cherry juice was sonicated at varying amplitude levels (50, 75, 100%); moderate temperatures (20, 30, 40 ℃); and treatment times of 2, 6, 10 min at a constant frequency of 20 kHz. Different ultrasonication amplitudes, temperatures, and times had no significant effect on pH,°Bx, and titratable acidity...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28473385/draft-whole-genome-sequences-of-multidrug-resistant-escherichia-coli-o157-h7-strains-isolated-from-feedlot-cattle-treated-with-growth-promoting-agents
#20
Muhammad A Rehman, Catherine Carrillo, François Malouin, Moussa S Diarra
Enterohemorrhagic Escherichia coli serotype O157:H7 is a major cause of foodborne outbreaks and hemolytic-uremic syndrome. Here, we report the draft genome sequences of three antibiotic-resistant E. coli O157:H7 strains isolated from feedlot cattle. These draft genome sequences will aid in the development of sequence-based tools for the detection of virulence and antimicrobial resistance genotypes.
May 4, 2017: Genome Announcements
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