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https://www.readbyqxmd.com/read/29342189/the-microbiota-metabolite-indole-inhibits-salmonella-virulence-involvement-of-the-phopq-two-component-system
#1
Nandita Kohli, Zeni Crisp, Rebekah Riordan, Michael Li, Robert C Alaniz, Arul Jayaraman
The microbial community present in the gastrointestinal tract is an important component of the host defense against pathogen infections. We previously demonstrated that indole, a microbial metabolite of tryptophan, reduces enterohemorrhagic Escherichia coli O157:H7 attachment to intestinal epithelial cells and biofilm formation, suggesting that indole may be an effector/attenuator of colonization for a number of enteric pathogens. Here, we report that indole attenuates Salmonella Typhimurium (Salmonella) virulence and invasion as well as increases resistance to colonization in host cells...
2018: PloS One
https://www.readbyqxmd.com/read/29341217/acetoacetate-and-ethyl-acetoacetate-as-novel-inhibitors-of-bacterial-biofilm
#2
S M Horne, M Schroeder, J Murphy, B M Prüβ
Acetoacetate (AAA) was identified as a biofilm inhibitor in a previous study, where the effect of 190 carbon and nitrogen sources on biofilm amounts by Escherichia coli O157:H7 was determined. With this study, we tested the effect of AAA on growth and biofilm amounts of Cronobacter sakazakii, Serratia marcescens, and Yersinia enterocolitica. AAA reduced growth and biofilm amounts of the three pathogens, albeit at rather high concentrations of 10 to 35 mg ml-1 . AAA at a concentration of 5 mg ml-1 reduced Y...
January 16, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29338194/sequential-and-selective-detection-of-two-molecules-with-a-single-solid-contact-chronopotentiometric-ion-selective-electrode
#3
Jiawang Ding, Nana Yu, Xuedong Wang, Wei Qin
A polymeric membrane ion-selective electrode (ISE) is typically designed for determination of one specific ion using conventional method. In this work, we demonstrate a simple, versatile and sensitive platform for simultaneous detection of two molecules with a single ISE. Under a series of periodic galvanostatic polarization, a solid-contact ISE without ion exchanger properties under zero-current conditions has been successfully used for simultaneous detection of two opposite charged ions with high sensitivity, good selectivity and fast reversibility...
January 17, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29337344/microbiological-quality-and-safety-of-fresh-fruits-and-vegetables-at-retail-levels-in-korea
#4
Charles Nkufi Tango, Shuai Wei, Imran Khan, Mohammad Shakhawat Hussain, Paul-François Ngnitcho Kounkeu, Joong-Hyun Park, Se-Hun Kim, Deog Hwan Oh
The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29336911/-detection-and-characterization-of-shiga-toxin-producing-escherichia-coli-in-children-treated-at-an-inter-zonal-pediatric-hospital-in-the-city-of-la-plata
#5
Sebastián Oderiz, Gerardo A Leotta, Lucía Galli
Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen that can cause watery diarrhea, bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). The objective of this study was to determine the phenotypic and genotypic profiles of STEC strains isolated from children with BD and HUS treated at a pediatric hospital in the city of La Plata in the period 2006-2012, and to establish the clonal relationship of O157:H7 isolates by pulsed field electrophoresis. The percentage of positive samples was 4...
January 11, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29328809/methods-for-the-control-of-foodborne-pathogens-in-low-moisture-foods
#6
Alma Fernanda Sánchez-Maldonado, Alvin Lee, Jeffrey M Farber
Low-moisture foods (LMFs) have been defined as those food products with a water activity (aw) less than 0.85 and are generally considered less susceptible to microbial spoilage and the growth of foodborne pathogens. However, in recent years, outbreaks linked to LMFs have increased, with Salmonella spp., Bacillus cereus, Cronobacter sakazakii, Clostridium spp., Escherichia coli O157:H7, non-O157 E. coli, and Staphylococcus aureus being the principal pathogens involved. Because of the new concerns raised as a result of recent outbreaks, new approaches need to be developed to control foodborne pathogens in LMFs...
January 12, 2018: Annual Review of Food Science and Technology
https://www.readbyqxmd.com/read/29326391/zoonotic-bacteria-and-parasites-found-in-raw-meat-based-diets-for-cats-and-dogs
#7
Freek P J van Bree, Gertie C A M Bokken, Robin Mineur, Frits Franssen, Marieke Opsteegh, Joke W B van der Giessen, Len J A Lipman, Paul A M Overgaauw
Feeding raw meat-based diets (RMBDs) to companion animals has become increasingly popular. Since these diets may be contaminated with bacteria and parasites, they may pose a risk to both animal and human health. The purpose of this study was to test for the presence of zoonotic bacterial and parasitic pathogens in Dutch commercial RMBDs. We analysed 35 commercial frozen RMBDs from eight different brands. Escherichia coli serotype O157:H7 was isolated from eight products (23 per cent) and extended-spectrum beta-lactamases-producing E coli was found in 28 products (80 per cent)...
January 13, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29320233/antibacterial-activities-of-hibiscus-sabdariffa-extracts-and-chemical-sanitizers-directly-on-green-leaves-contaminated-with-foodborne-pathogens
#8
Carlos A Gómez-Aldapa, Esmeralda Rangel-Vargas, Ma Refugio Torres-Vitela, Angélica Villarruel-López, Otilio A Acevedo-Sandoval, Alberto J Gordillo-Martínez, Angélica Godínez-Oviedo, Javier Castro-Rosas
Leafy greens have been associated with foodborne disease outbreaks in different countries. To decrease microbial contamination of leafy greens, chemical agents are commonly used; however, a number of studies have shown these agents to have limited antimicrobial effect against pathogenic bacteria on vegetables. The objective of this study was to compare the antibacterial effect of Hibiscus sabdariffa calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on leafy greens...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29315715/a-mathematical-model-for-pathogen-cross-contamination-dynamics-during-the-postharvest-processing-of-leafy-greens
#9
Amir Mokhtari, David Oryang, Yuhuan Chen, Regis Pouillot, Jane Van Doren
We developed a probabilistic mathematical model for the postharvest processing of leafy greens focusing on Escherichia coli O157:H7 contamination of fresh-cut romaine lettuce as the case study. Our model can (i) support the investigation of cross-contamination scenarios, and (ii) evaluate and compare different risk mitigation options. We used an agent-based modeling framework to predict the pathogen prevalence and levels in bags of fresh-cut lettuce and quantify spread of E. coli O157:H7 from contaminated lettuce to surface areas of processing equipment...
January 8, 2018: Risk Analysis: An Official Publication of the Society for Risk Analysis
https://www.readbyqxmd.com/read/29306732/sequential-effect-of-phages-and-cold-nitrogen-plasma-against-escherichia-coli-o157-h7-biofilms-on-different-vegetables
#10
Haiying Cui, Mei Bai, Lu Yuan, Duraiarasan Surendhiran, Lin Lin
Escherichia coli O157:H7 (E. coli O157:H7) is one of the most common pathogens in fresh vegetables and fruits, and most of the diseases produced by E. coli O157:H7 are associated with biofilms. Cold nitrogen plasma (CNP) is a cold sterilization technique which has no residue. However to completely eliminate the biofilm on the surface of vegetables the processing power and time of CNP have to be enhanced, which will impact on the quality of fruits and vegetables. Thus the sequential treatment of CNP and phage techniques was engineered in this study...
January 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29303175/nanoporous-anodic-aluminum-oxide-internalized-with-gold-nanoparticles-for-on-chip-pcr-and-direct-detection-by-surface-enhanced-raman-scattering
#11
B M Tran, N N Nam, S J Son, N Y Lee
Nanoporous anodic aluminum oxide internalized with gold nanoparticles was utilized as an integrated platform miniaturized for consecutively performing on-chip PCR and downstream detection of the amplified product of a 183 bp eaeA gene fragment from Escherichia coli O157:H7 using surface-enhanced Raman scattering (SERS).
January 5, 2018: Analyst
https://www.readbyqxmd.com/read/29300761/investigation-of-prevalence-of-free-shiga-toxin-producing-escherichia-coli-stec-specific-bacteriophages-and-its-correlation-with-stec-bacterial-hosts-in-a-produce-growing-area-in-salinas-california
#12
Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu
Shiga toxin-producing E. coli (STEC) causes approximately 265,000 illnesses and 3,600 hospitalizations annually and is highly associated with animal contamination due to the natural reservoir of ruminant gastrointestinal tracts. Free STEC-specific bacteriophages against STEC strains are also commonly isolated from fecal-contaminated environment. Previous studies have evaluated the correlation between the prevalence of STEC-specific bacteriophages and STEC strains to improve animal-associated environment. However, the similar information regarding free STEC-specific bacteriophages prevalence in produce growing area is lacking...
2018: PloS One
https://www.readbyqxmd.com/read/29284205/pathogenic-and-phylogenetic-characteristics-of-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-retail-meats-in-south-korea
#13
June Bong Lee, Dalmuri Han, Hyung Tae Lee, Seon Mi Wi, Jeong Hoon Park, Jeong Woo Jo, Young-Jae Cho, Tae-Wook Hahn, Sunjin Lee, Byunghak Kang, Hyo Sun Kwak, Jonghyun Kim, Jang Won Yoon
Herein, we report the pathogenic and phylogenetic characteristics of seven Shiga toxin (Stx)-producing Escherichia coli (STEC) isolates from 434 retail meats in Korea during 2006-2012. The experimental analyses revealed that all the isolates (i) were identified as non-O157 STEC, including O91:H14 (3 isolates), O121:H10 (2), O91:H21 (1), and O18:H20 (1), (ii) carried diverse Stx subtype genes (stx1, stx2c, stx2e, or stx1+stx2b) whose expression levels were varied strain by strain, and (iii) lacked the locus of enterocyte effacement (LEE) pathogenicity island, a major virulence factor of STEC, but possessed one or more alternative virulence genes encoding cytotoxins (Cdt and SubAB) and/or adhesins (Saa, Iha, and EcpA)...
December 28, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29280544/low-prevalence-of-the-gang-of-seven-and-absence-of-the-o80-h2-serotypes-amongst-shigatoxigenic-and-enteropathogenic-escherichia-coli-stec-and-epec-in-intestinal-contents-of-healthy-cattle-at-two-slaughterhouses-in-belgium-2014
#14
D Thiry, K De Rauw, S Takaki, J-N Duprez, A Iguchi, D Piérard, N Korsak, J G Mainil
AIMS: The purpose of this survey was to estimate the respective prevalence of the "gang of seven" and "non-gang of seven" serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. METHODS AND RESULTS: After overnight enrichment growth the 69 intestinal contents testing positive with PCR targeting the eae, stx1 and stx2 genes were inoculated onto four agar media...
December 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29280543/associations-between-escherichia-coli-o157-shedding-and-the-fecal-microbiota-of-dairy-cows
#15
Chloe Stenkamp-Strahm, Craig McConnel, Sheryl Magzamen, Zaid Abdo, Stephen Reynolds
AIMS: Dairy cattle shed pathogenic E. coli O157 (O157) in feces, playing a role in human exposure. We aimed to measure fecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. METHODS AND RESULTS: Daily fecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene sequencing was used to measure sample-level microbial communities. Alpha diversity metrics were associated with O157 outcomes via regression modeling, adjusting for confounders...
December 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29280319/importance-of-the-rpoe-regulon-in-maintaining-the-lipid-bilayer-during-antimicrobial-treatment-with-the-polycationic-agent-chlorhexidine
#16
Sinisa Vidovic, Prabhakara Medihala, James J Dynes, Prasad Daida, Vladimir Vujanovic, Adam P Hitchcock, Deeksha Shetty, Haixia Zhang, David R Brown, John R Lawrence, Darren R Korber
The emergence of multidrug resistance has reached alarming levels. To solve this growing problem, discovery of novel cellular targets or pathways important for antimicrobial resistance is urgently needed. In this study, we explored how the alternative sigma factor, RpoE, protects Escherichia coli O157 against the toxic effects of the polycationic antimicrobial agent, chlorhexidine (CHX). Susceptibility of this organism to CHX was found to directly correlate to the growth rate, with the faster-replicating wild-type being more susceptible to CHX than its more slowly-replicating ΔrpoE O157 mutant...
December 26, 2017: Proteomics
https://www.readbyqxmd.com/read/29279448/comparison-of-three-molecular-subtyping-methods-among-o157-and-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-japanese-cattle
#17
Hiromi Nakamura, Atsushi Iguchi, Tomofumi Maehara, Kyohei Fujiwara, Atsushi Fujiwara, Jun Ogasawara
To determine the infection source, route, and extent of outbreak, it is important to subtype Shiga toxin-producing Escherichia coli (STEC) isolates belonging to the same serotype for clustering into clonally related groups. In this study, we compared three molecular subtyping methods, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and multiple-locus variable number of tandem repeat analysis (MLVA), using O157 and non-O157 STEC isolated from Japanese beef cattle. A total of 73 STEC isolates belonging to 9 O-serogroups were used...
December 26, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29278379/a-comparison-study-of-quality-attributes-of-ground-beef-and-veal-patties-and-thermal-inactivation-of-escherichia-coli-o157-h7-after-double-pan-broiling-under-dynamic-conditions
#18
KaWang Li, Amanda Gipe McKeith, Cangliang Shen, Russell McKeith
This study compared the quality variation and thermal inactivation of Escherichia coli O157:H7 in non-intact beef and veal. Coarse ground beef and veal patties (2.1 cm thick, 12.4 cm diameter, 180 g) inoculated with E. coli O157:H7, aerobically stored before double pan-broiling for 0-360 s without rest or to 55, 62.5, 71.1, and 76 °C (internal temperature) with 0.5- or 3.5-min rest. Microbial population and qualities including color, cooking losses, pH, water activity, fat, and moisture content, were tested...
December 26, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29276663/detection-of-viable-but-non-culturable-escherichia-coli-o157-h7-by-pcr-in-combination-with-propidium-monoazide
#19
Junliang Zhong, Xihong Zhao
The aim of this study was to evaluate the applicability of the conventional PCR detection method combined with propidium monoazide (PMA) treatment for the detection of viable but non-culturable (VBNC) state Escherichia coli O157:H7 in ground beef meatballs. Under low temperature, E. coli O157:H7 cells were induced into the VBNC state in ground beef meatballs at - 20 °C after 152 days. The optimal PMA concentration of 5 µg/mL was obtained in beef meatball samples, which could completely inhibit the DNA amplification on dead cells (106 cells/mL) but with no inhibition on viable cells...
January 2018: 3 Biotech
https://www.readbyqxmd.com/read/29276661/microencapsulation-of-reuterin-to-enhance-long-term-efficacy-against-food-borne-pathogen-listeria-monocytogenes
#20
Santosh Kumar Mishra, R K Malik, Harsh Panwar, Amit Kumar Barui
The aim of this study was to microencapsulate the reuterin produced by Lactobacillus reuteri BPL-36 strain for its long-term efficacy against food-borne pathogen Listeria monocytogenes. Lactobacillus reuteri BPL-36 strain previously isolated from a human infant fecal sample in lab was selected for the present study based on its ability to produce reuterin. The organism displayed a broad-spectrum antimicrobial activity. Reuterin concentration of 89.63 mM was obtained in the MRS-glycerol medium after 16 h incubation at 37 °C...
January 2018: 3 Biotech
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