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Ji Hoon Kang, Kyung Bin Song
An aqueous chlorine dioxide (ClO₂) treatment combined with highly activated calcium oxide (CaO) and mild heat inactivated naturally existing bacteria and Escherichia coli O157:H7 inoculated on fresh-cut kale. Kale samples were treated with different concentrations of ClO₂ (10, 30, and 50 ppm), CaO (0.01, 0.05, 0.1, and 0.2%), and mild heat (25, 45, 55, and 65°C) as well with combinations of 30 or 50 ppm ClO₂ and 0.2% CaO at 55°C for 3 min. An increasing concentration of ClO₂ and CaO significantly reduced the microbial population compared to the control...
October 25, 2016: Journal of Microbiology and Biotechnology
Jennyka Hallewell, Tim Reuter, Kim Stanford, Ed Topp, Trevor W Alexander
The goal of this study was to monitor Shiga toxin-producing Escherichia coli (STEC) serogroups and virulence genes in cattle (n = 30) originating from a closed herd. Fecal samples were collected (1) at weaning, (2) upon arrival to a feedlot, (3) after 30 days on feed (DOF), and (4) after 135 DOF. DNA was extracted from feces for detection of virulence and serogroup genes by polymerase chain reaction (PCR) and immunomagnetic separation and pulsed-field gel electrophoresis (PFGE) were performed to collect and subtype STEC isolates...
October 25, 2016: Foodborne Pathogens and Disease
G Kamkamidze, I Migriauli, D Razmadze, M Kochlamazashvili, K Mulkijanyan, M Butsashvili
The main objective of this investigation was to develop and pilot a real-time Polymerase Chain Reaction (rt-PCR) diagnostic system for rapid and simultaneous identification of pathogens with a particular emphasis on diarrheal disease diagnostics. The diarrheal diseases were selected as a target for the pilot because they constitute a primary public health priority in Georgia and worldwide. The product developed by our research team "Neo_PCR_Diagnostics" represents an original system for the identification of pathogens associated with gastrointestinal tract infections and diarrhea...
September 2016: Georgian Medical News
Gitishree Das, Jayanta Kumar Patra, Kwang-Hyun Baek
Endophytic bacteria (EB) are a rich source of secondary metabolites with medicinal importance. In this study, EB were isolated from the bottle brush herb Equisetum arvense and identified based on 16S rRNA sequencing. Evaluation of its antibacterial potential was conducted using two common foodborne pathogenic bacteria, Staphylococcus aureus ATCC 12600 and Escherichia coli O157:H7 ATCC 43890. Out of 103 identified EB, three species, Streptomyces albolongus, Dermacoccus sp., and Mycobacterium sp., showed significant antibacterial activity against S...
October 18, 2016: Foodborne Pathogens and Disease
H W Kim, Y J Hong, J I Jo, S D Ha, S H Kim, H J Lee, M S Rhee
Microbiological quality of 206 raw ready-to-eat seafood samples were investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster, and squid) and distribution channels (fishery, hyper, and online market). Enumeration of Aerobic Plate Count (APC) and total coliforms (TC) and pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus) were performed, and level of microbiological quality were classified into four groups: satisfactory, acceptable, unsatisfactory, and unacceptable...
October 16, 2016: Letters in Applied Microbiology
Yosra Ben-Fadhel, Sabrina Saltaji, Mohamed Ali Khlifi, Stephane Salmieri, Khanh Dang Vu, Monique Lacroix
The antimicrobial activity of essential oils (EOs), organic acid (OA) salts and natamycin, a natural antifungal produced during fermentation by the bacterium Streptomyces natalensis, was assessed against four pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Aspergillus niger). The Minimum Inhibitory Concentration (MIC) of each antimicrobial (AM) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. Then, the interactions between different antimicrobial compounds showing the lowest MIC were determined by the checkerboard method...
October 11, 2016: International Journal of Food Microbiology
Indira T Kudva, Michelle Q Carter, Vijay K Sharma, Judith A Stasko, Jorge A Giron
: Our recent studies have shown that Intimin and the Locus of Enterocyte Effacement-encoded proteins do not play a role in Escherichia coli O157:H7 (O157) adherence to the bovine recto-anal junction squamous epithelial (RSE) cells. To define factors that play a contributory role, we investigated the role of curli, a fimbrial adhesin commonly implicated in adherence to various fomites, plant, and human epithelial cells, in O157 adherence to RSE cells. Specifically, we examined (i) wild-type strains of O157; (ii) curli variants of O157 strains; (iii) isogenic curli-deletion mutants of O157; and (iv) adherence-inhibition of O157 using anti-curlin sera...
October 14, 2016: Applied and Environmental Microbiology
Yingying Hong, Jyothi Thimmapuram, Jiayi Zhang, Clayton K Collings, Ketaki Bhide, Kyle Schmidt, Paul D Ebner
Numerous studies have shown the efficacy of phage therapy in reducing foodborne pathogen carriage in food animals. Fewer studies have focused on host reactions, especially in terms of phage-mediated acute immune responses and effects on the gut microbiome. Here we administered E. coli O157:H7 phages in low (single dose of 10(5) PFU) or high (single dose of 10(7) PFU) quantities to mice. While there were time points at which cytokine levels in different treatment groups differed from one another, all cytokine levels remained within normal ranges for mice regardless of treatment...
July 2016: Bacteriophage
Maryoris E Soto Lopez, Laís Silva Batalha, Pedro Marcus Pereira Vidigal, Luiz Augusto A Albino, Delaine Meireles Gouveia Boggione, Marco Tulio Pardini Gontijo, Denise M Soares Bazzolli, Regina C Santos Mendonca
Here, we present the genome sequence of the Escherichia coli bacteriophage UFV-AREG1. This phage was isolated from cowshed wastewater and showed specificity for enterohemorrhagic E. coli O157:H7 (ATCC 43895), E. coli 0111 (CDC O11ab) and E. coli (ATCC 23229).
October 13, 2016: Genome Announcements
Tuhina Banerjee, Shoukath Sulthana, Tyler Shelby, Blaze Heckert, Jessica Jewell, Kalee Woody, Vida Karimnia, James McAfee, Santimukul Santra
Enterohemorrhagic Escherichia coli O157:H7 presents a serious threat to human health and sanitation and is a leading cause in many food- and waterborne ailments. While conventional bacterial detection methods such as PCR, fluorescent immunoassays and ELISA exhibit high sensitivity and specificity, they are relatively laborious and require sophisticated instruments. In addition, these methods often demand extensive sample preparation and have lengthy readout times. We propose a simpler and more sensitive diagnostic technique featuring multiparametric magneto-fluorescent nanosensors (MFnS)...
October 14, 2016: ACS Infectious Diseases
Mithila Ferdous, Anna M D Kooistra-Smid, Kai Zhou, John W A Rossen, Alexander W Friedrich
Escherichia coli (E.coli) O157 that do not produce Shiga toxin and do not possess flagellar antigen H7 are of diverse H serotypes. In this study, the antibiotic resistance properties, genotype of a set of virulence associated genes and the phylogenetic background of E. coli O157:non-H7 groups were compared. Whole genome sequencing was performed on fourteen O157:non-H7 isolates collected in the STEC-ID-net study. The genomes were compared with E. coli O157 genomes and a typical Enteropathogenic E. coli (tEPEC) genome downloaded from NCBI...
2016: Frontiers in Microbiology
Fengying Dai, Miao Zhang, Dixin Xu, Yin Yang, Jiaxiao Wang, Mingzhen Li, Meihong Du
Micro- and nano-immunomagnetic beads (MIMBs and NIMBs) used for immunomagnetic separation (IMS) with Polymerase Chain Reaction (PCR) were studied for the rapid detection of Salmonella. The capture efficiency of the two different IMBs was evaluated by a conventional plate counting method, and the binding pattern was studied by scanning electron microscopy. The specificity of the IMBs was tested with Salmonella, Shigella flexneri, Enterhemorrhagic Escherichia coli O157:H7(EHEC O157:H7) and Listeria monocytogenes...
October 12, 2016: Biotechnology and Applied Biochemistry
Haiying Cui, Cuixia Ma, Lin Lin
E. coli O157:H7 is a bacterium frequently found on vegetable surfaces, such as cucumber, that can pose a significant threat to consumers. In the present work, proteinase K (PK) and thyme oil (TO) were used to inactivate E. coli O157:H7 biofilms. Both PK and TO were encapsulated in liposomes to improve their chemical stability and to allow for a controlled release. The optimal PK/TO-loaded liposomes (particle size of 170.4 nm, polydispersity index of 0.309, zeta potential of -29.8 mV and entrapment efficiency of 33...
September 14, 2016: Food & Function
Vivek Sharma, Taranpreet Kaur, Helen Bridle, Moushumi Ghosh
This study evaluated five extracellular polymers of bacterial origin possessing mucoadhesive properties for their antimicrobial properties and toxicological characteristics. Of the five tested mucoadhesive biopolymers, the extracellular polymer produced by a strain of Acinetobacter haemolyticus exhibited broad antimicrobial efficacy towards Yersinia enterocolitica, Salmonella typhimurium, Listeria monocytogenes, Escherichia coli O157:H7 and Bacillus subtilis. Significant (p<0.05) inhibition of gram negative bacterial pathogens followed by gram positives were observed with the biopolymer at a dose of 40-60μg ml-1 at ambient temperature...
October 6, 2016: International Journal of Biological Macromolecules
V Ntuli, P M K Njage, E M Buys
The current study was undertaken to characterize Escherichia coli and other Enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (PDBM). A total of 258 samples were collected from purchase points in 8 provinces in South Africa. The samples were tested for antibiotic residues, phosphatase, total aerobic bacteria, coliforms, and E. coli counts. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for identification of isolates. Escherichia coli isolates were characterized for virulence factors, antimicrobial resistance, serotypes, and presumptive E...
October 5, 2016: Journal of Dairy Science
Hae In Yong, Haelim Lee, Sanghoo Park, Jooyoung Park, Wonho Choe, Samooel Jung, Cheorun Jo
The aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. After plasma treatment for 10min, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3Log CFU/g. No significant changes in metmyoglobin content, shear force, and myofibrillar fragmentation index were found in the plasma-treated beef jerky...
September 30, 2016: Meat Science
Pamela Vazquez-Gutierrez, Tomas de Wouters, Julia Werder, Christophe Chassard, Christophe Lacroix
The gut microbiota plays an important role in host health, in particular by its barrier effect and competition with exogenous pathogenic bacteria. In the present study, the competition of Bifidobacterium pseudolongum PV8-2 (Bp PV8-2) and Bifidobacterium kashiwanohense PV20-2 (Bk PV20-2), isolated from anemic infant gut microbiota and selected for their high iron sequestration properties, was investigated against Salmonella Typhimurium (S. Typhi) and Escherichia coli O157:H45 (EHEC) by using co-culture tests and assays with intestinal cell lines...
2016: Frontiers in Microbiology
Pauline Bondue, Sébastien Crèvecoeur, François Brose, Georges Daube, Marie-Christine Seghaye, Mansel W Griffiths, Gisèle LaPointe, Véronique Delcenserie
Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and B. crudilactis, a species of bovine origin, is able to metabolize some of those complex carbohydrates...
2016: Frontiers in Microbiology
Eun-Jin Kim, Hyun-Joo Chang, Soojin Kwak, Jong-Hyun Park
Characteristics of E.coli O157:H7 specific infection bacteriophages (O157 coliphages) and broad-host-range bacteriophages for other E.coli serotypes (broad-host coliphages) were compared. The burst sizes of two groups were ranged from 40 to 176 PFU/infected. Distribution of virulence factors of stx1, stx2, ehxA, and saa between two groups were not differenciated. Broad host coliphages showed a little stable at 70°C than O157 coliphages. However, O157 coliphages showed high acid and ethanol tolerant by reduction of only 22% and 11% phages under pH 3 and 70% ethanol for an hour exposure...
October 6, 2016: Journal of Microbiology and Biotechnology
Hyeon-Jeong Son, Kyung Bin Song
This study examined the antimicrobial activity of flaxseed meal extract (FME) against Staphylococcus aureus and Escherichia coli O157:H7 inoculated on red mustard. With the treatment of 0.7% FME for 3 min, the reduction levels of S. aureus and E. coli O157:H7 populations were 1.23 and 1.83 log CFU/g, respectively. In addition, the combined treatment of 0.7% FME at 50°C for 3 min reduced the populations of the pathogenic bacteria by 2.28 and 2.41 log CFU/g, respectively. Color and vitamin C content were not significantly different between treatments...
October 6, 2016: Journal of Microbiology and Biotechnology
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