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https://www.readbyqxmd.com/read/28630205/the-impact-of-gyrb-and-eis-mutations-in-improving-second-line-drug-resistance-detection-among-mycobacterium-tuberculosis-isolates-from-georgia
#1
N Bablishvili, N Tukvadze, E Shashkina, B Mathema, N R Gandhi, H M Blumberg, R R Kempker
SETTING: The country of Georgia has a high burden of multi- and extensively drug-resistant tuberculosis (XDR-TB).OBJECTIVE: To evaluate whether the detection of mutations in gyrB and eis genes increased the sensitivity of detecting phenotypic resistance to ofloxacin and kanamycin or capreomycin as compared to use of the first generation MTBDRsl assay alone which evaluates for mutations in gyrA and rrs genes.DESIGN: A retrospective study of storedMycobacterium tuberculosis isolates. All isolates underwent DNA sequencing of resistance determining regions...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28623303/xtractb-assay-a-mycobacterium-tuberculosis-molecular-screening-test-with-sensitivity-approaching-culture
#2
Jennifer L Reed, Debby Basu, Matthew A Butzler, Sally M McFall
Nucleic acid amplification tests are increasingly used to diagnose tuberculosis (TB) due to their speed and sensitivity compared to sputum smear microscopy. However, these tests fail to equal culture's sensitivity with sputum smear microscopy negative specimens and therefore cannot be used to rule out TB disease. For molecular tests to match culture's sensitivity, they must detect ≤10 genomic copies of Mycobacterium tuberculosis (MTB) DNA, the limit of detection of culture, process ≥1 ml of sputum ensuring sufficient number of MTB are in the reaction, and efficiently remove sputum associated inhibitors from this large sample...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616904/-the-diagnostic-value-of-endobronchial-ultrasound-guided-needle-aspiration-biopsy-for-lung-or-mediastinal-lymph-node-cancer-and-tuberculosis
#3
Ye Wang, Hui Zhu, Sai Yang, Ke Wang, Pan-Wen Tian, Jing-Yu Shi, Qing-Bing Han, Yong-Xiao Luo, Feng-Min Luo, Wei-Min Li
OBJECTIVES: To determine the value of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in diagnosing lung or mediastinal lymph node cancer and tuberculosis. METHODS: Clinical and pathological data of 553 patients who underwent EBUS-TBNA from January 2013 to September 2016 in West China Hospital of Sichuan University were reviewed. The sensitivity, specificity and accuracy of EBUS-TBNA for diagnosing lymph node tumor and tuberculosis of hilar and mediastinal lymph nodes were calculated...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28614380/interleukin-34-inhibits-hepatitis-b-virus-replication-in-vitro-and-in-vivo
#4
Sheng-Tao Cheng, Hua Tang, Ji-Hua Ren, Xiang Chen, Ai-Long Huang, Juan Chen
BACKGROUND: The hepatitis B virus (HBV) infection could activate the immune system and induce extensive inflammatory response. As the most important inflammatory factor, interleukins are critical for anti-viral immunity. Here we investigated whether interleukin-34 (IL-34) play a role in HBV infection. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we first found that both serum IL-34 and IL-34 mRNA in PBMCs in chronic HBV patients was significantly decreased compared to the healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28612535/-prediction-and-analysis-of-epitopes-in-clpp2-of-mycobacterium-tuberculosis
#5
Si-Jing Liu, Ming-Juan Jiang, Qi-Kang Pu, Chen-Yan Ren, Lin Su, Xiang Zhang, Chuan Wang
OBJECTIVES: To predict and analyze the antigenic epitopes in Mycobacterium tuberculosis protein caseinolytic protease P2 (clpP2), and explore its possibility to be applied as a new tuberculosis (TB) vaccine and drug development target. METHODS: Secondary structure of clpP2 based on nucleic sequence was predicted by DNA Star software. The homologous sequence conformation were analyzed by Swiss-Model online software. T cells antigenic epitopes were predicted through VaxiPred, and B cell epitopes were predicted by combining use of several different prediction programs, such as ABCpred, COBEPro and BepiPredPred...
March 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28610793/a-novel-automatic-molecular-test-for-detection-of-multidrug-resistance-tuberculosis-in-sputum-specimen-a-case-control-study
#6
Qiang Li, Xi C Ou, Yu Pang, Hui Xia, Hai R Huang, Bing Zhao, Sheng F Wang, Yan L Zhao
MiniLab tuberculosis (ML TB) assay is a new automatic diagnostic tool for diagnosis of multidrug resistance tuberculosis (MDR-TB). This study was conducted with aims to know the performance of this assay. Sputum sample from 224 TB suspects was collected from tuberculosis suspects seeking medical care at Beijing Chest hospital. The sputum samples were directly used for smear and ML TB test. The left sputum sample was used to conduct Xpert MTB/RIF, Bactec MGIT culture and drug susceptibility test (DST). All discrepancies between the results from DST, molecular and phenotypic methods were confirmed by DNA Sequencing...
July 2017: Tuberculosis
https://www.readbyqxmd.com/read/28592925/second-generation-multiple-reaction-monitoring-assays-for-enhanced-detection-of-ultra-low-abundance-mycobacterium-tuberculosis-peptides-in-human-serum
#7
Carolina Mehaffy, Karen M Dobos, Payam Nahid, Nicole A Kruh-Garcia
BACKGROUND: Mycobacterium tuberculosis (Mtb) is the causative agent of Tuberculosis (TB), the number one cause of death due to an infectious disease. TB diagnosis is performed by microscopy, culture or PCR amplification of bacterial DNA, all of which require patient sputum or the biopsy of infected tissue. Detection of mycobacterial products in serum, as biomarkers of diagnosis or disease status would provide an improvement over current methods. Due to the low-abundance of mycobacterial products in serum, we have explored exosome enrichment to improve sensitivity...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28592346/analysing-tuberculosis-cases-among-healthcare-workers-to-inform-infection-control-policy-and-practices
#8
Gerard de Vries, Rianne van Hunen, Fleur S Meerstadt-Rombach, Paul D L P M van der Valk, Marinus Vermue, Sytze T Keizer
OBJECTIVE To determine the number and proportion of healthcare worker (HCW) tuberculosis (TB) cases infected while working in healthcare institutions in the Netherlands and to learn from circumstances that led to these infections. DESIGN Cohort analysis. METHODS We included all HCW TB patients reported to the Netherlands TB Register from 2000 to 2015. Using data from this register, including DNA fingerprints of the bacteria profile and additional information from public health clinics, HCW TB cases were classified into 4 categories: (1) infected during work in the Netherlands, (2) infected in the community, (3) infected outside the Netherlands, or (4) outside these 3 categories...
June 8, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28577597/enhanced-dna-sensitized-tb-3-luminescence-in-organic-solvents-for-more-sensitive-detection
#9
Li Xu, Wenhu Zhou, Juewen Liu
DNA-sensitized Tb(3+) luminescence spectroscopy is a powerful method for probing nucleic acids and developing biosensors. Its performance in organic solvents has yet to be explored. In this study, Tb(3+) luminescence with nucleosides, nucleotides and DNA oligonucleotides in various organic solvents is studied. Tb(3+) emission with single nucleotides is quenched up to 88% in dimethyl formamide (DMF), while its emission with nucleosides is enhanced. For the four 15-mer DNA homopolymers, the strongest absolute emission enhancement was achieved with C15...
July 18, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28574018/genetic-diversity-drug-sensitivity-profiles-of-mycobacterium-tuberculosis-isolates-from-two-slums-of-jaipur-city-rajasthan-india
#10
Bharti Malhotra, Deepti Dashora, Vipin Kumar, Sumit Goyal, Bhavana Sharma, Madhu Kumar, Kailash Narayan Gupta, Vishnu Dutt Sharma, D S Chauhan, Kiran Katoch, Vishwa Mohan Katoch
BACKGROUND & OBJECTIVES: Slums are considered as hotspots of tuberculosis (TB). The study of genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis (MTB) will help understand the transmission dynamics and can be used for better prevention and control of the disease. The aim of this study was to determine the drug susceptibility profiles and genetic diversity using the random amplified polymorphic DNA (RAPD) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU VNTR) of MTB isolates from sputum samples of pulmonary TB patients residing in the two slums of Jaipur city in Rajasthan, India...
January 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28565846/use-of-dna-microarray-chips-for-the-rapid-detection-of-mycobacterium-tuberculosis-resistance-to-rifampicin-and-isoniazid
#11
Peijun Tang, Xiafang Wang, Xinghua Shen, Meihua Shi, Xuefeng Zhu, Xin Yu, Jia Liu, Chunhua Ling, Meiying Wu
The objective of the present study was to evaluate the potential development of DNA microarray chips to detect rifampicin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB), using samples from clinical tuberculosis (TB) patients in Soochow City, China. The sputum samples of 42 patients with TB in the Affiliated Hospital of Infectious Diseases of Soochow University (Soochow, China) were collected. The conventional Lowenstein-Jensen culture medium (Gold Standard) was used to assess drug sensitivity using the absolute concentration method...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28565753/clinical-and-genomic-responses-to-ultra-short-course-chemotherapy-in-spinal-tuberculosis
#12
Ningkui Niu, Qian Wang, Jiandang Shi, Xu Zhang, Guangqi Geng, Shufeng Zhou, Chia Thach, Feng Cheng, Zili Wang
Traditional treatments for spinal tuberculosis (TB) involve chemotherapy and surgery. In the present study, it has been identified that chemotherapy lasting <6 months [ultra-short course chemotherapy (UCCT)], rather than the 6-18 months of the traditional regimen, is effective in sustaining TB clearance following complete surgical debridement. This current study aims to compare the changes in peripheral blood gene expression prior to and following UCCT, subsequent to complete debridement of spinal TB lesions...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28551117/electrochemical-determination-of-m-tuberculosis-antigen-based-on-poly-3-4-ethylenedioxythiophene-and-functionalized-carbon-nanotubes-hybrid-platform
#13
Himkusha Thakur, Navpreet Kaur, Dipti Sareen, Nirmal Prabhakar
An electrochemical DNA aptasensor for the detection of Mycobacterium tuberculosis (M. tb) antigen MPT64, was developed using Poly(3,4-ethylenedioxythiophene) (PEDOT) doped with carbon nanotubes (CNTs). The biotinylated aptamer was immobilized onto streptavidin attached to -COOH functionalized CNTs via streptavidin-biotin interaction. Various characterization studies as FT-IR, FE-SEM, EIS and DPV were done to validate each fabrication step of the aptasensor. Optimization studies related to aptamer concentration and response time were performed...
August 15, 2017: Talanta
https://www.readbyqxmd.com/read/28535727/differential-carriage-of-virulence-associated-loci-in-the-new-zealand-rangipo-outbreak-strain-of-mycobacterium-tuberculosis
#14
Sanjay S Gautam, Micheál Mac Aogáin, James E Bower, Indira Basu, Ronan F O'Toole
BACKGROUND: The Rangipo strain of Mycobacterium tuberculosis achieved notoriety in New Zealand due to its role in several tuberculosis (TB) outbreaks. Why this strain should be the source of relatively large clusters of the disease is unknown. In this work, we performed an in-depth analysis of the genome of the Rangipo strain to determine whether it offers clues to understanding its prevalence. METHODS: Next-generation sequencing was performed on nine isolates which matched the Rangipo genotypic profile...
May 23, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28534565/lanthanoplatins-emissive-eu-iii-and-tb-iii-complexes-staining-nucleoli-targeted-through-pt-dna-crosslinking
#15
Khushbu Singh, Swati Singh, Payal Srivastava, Sri Sivakumar, Ashis K Patra
Two highly luminescent water-soluble heterometallic LnPt2 complexes, [{cis-PtCl(NH3)2}2Ln(L)(H2O)](NO3)2 (Ln = Eu (1), Tb (2)), have been designed for their selective nucleoli staining through formation of Pt-DNA crosslinks. The complexes showed significant cellular uptake and distinctive nucleoli localization through intrinsic emission from Eu(III) or Tb(III) observed through confocal fluorescence microscopy.
June 1, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28522247/potential-of-multi-component-antigens-for-tuberculosis-diagnosis
#16
Sana Khurshid, Madeeha Afzal, Ruqqya Khalid, M Waheed Akhtar, Mahmood Husain Qazi
Tuberculosis is one of the top ten causes of deaths worldwide. The cause of tuberculosis is a bacterium, Mycobacterium tuberculosis, which has been surviving for centuries. Immunological tests based on detecting the presence of antibodies in the sera of active TB patients against various antigens of M. tuberculosis are useful for diagnosis of TB and offer simple, rapid and cost effective methods most suitable for poor and developing countries. Several recombinant antigens have been reported so far with varying sensitivity individually, yet none had shown sensitivity higher enough to be used in a commercial test...
May 15, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28495330/relationships-between-age-and-microarchitectural-descriptors-of-iliac-trabecular-bone-determined-by-microct
#17
C Deguette, A Ramond-Roquin, C Rougé-Maillart
AIM: Estimation of age at death is a major issue in anthropology. The main anthropological histological methods propose studying the architecture of cortical bone. In bone histomorphometry, researches on metabolic bone diseases have provided normative tables for trabecular bone volume (BV/TV) according to age and gender of individuals on trans-iliac bone biopsies. We have used microCT, a non-destructive tool for measuring bone volume and trabecular descriptors to compare the French tables to a series of forensic anthropological population and if the two iliac bones could be used interchangeably...
May 8, 2017: Morphologie: Bulletin de L'Association des Anatomistes
https://www.readbyqxmd.com/read/28487230/assessment-of-antigenic-supplementation-of-bovine-purified-protein-derivative-for-diagnosis-of-subclinical-infection-with-mycobacterium-bovis-in-cattle
#18
A H Alvarez, A Gutiérrez-Ortega, V Gómez-Entzin, G Pérez-Mayorga, J Naranjo-Bastién, V González-Martínez, F Milián-Suazo, M Martínez-Velázquez, S Herrera-Rodríguez, E Hinojoza-Loza
Bovine tuberculosis (bTB) is usually diagnosed in vivo and ex vivo on the basis of delayed hypersensitivity reactions with a complex pool of antigens named bovine tuberculin (PPDB). The IFN-γ release assay (IGRA) for bTB is a blood-based assay that improves detection of infected cattle at early stages that escape skin testing. Improvements to IFN-γ testing with specific proteins have been performed to increase sensitivity. DosR regulon-related antigens are well known mycobacterial proteins expressed during the non-replicative phases of infection, this has been useful to improve the diagnosis of subclinical forms of TB in suspected individuals...
July 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28482027/cmr-is-a-redox-responsive-regulator-of-dosr-that-contributes-to-m-tuberculosis-virulence
#19
Laura J Smith, Aleksandra Bochkareva, Matthew D Rolfe, Debbie M Hunt, Christina Kahramanoglou, Yvonne Braun, Angela Rodgers, Alix Blockley, Stephen Coade, Kathryn E A Lougheed, Nor Azian Hafneh, Sarah M Glenn, Jason C Crack, Nick E Le Brun, José W Saldanha, Vadim Makarov, Irene Nobeli, Kristine Arnvig, Galina V Mukamolova, Roger S Buxton, Jeffrey Green
Mycobacterium tuberculosis (MTb) is the causative agent of pulmonary tuberculosis (TB). MTb colonizes the human lung, often entering a non-replicating state before progressing to life-threatening active infections. Transcriptional reprogramming is essential for TB pathogenesis. In vitro, Cmr (a member of the CRP/FNR super-family of transcription regulators) bound at a single DNA site to act as a dual regulator of cmr transcription and an activator of the divergent rv1676 gene. Transcriptional profiling and DNA-binding assays suggested that Cmr directly represses dosR expression...
May 8, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28469695/molecular-diagnosis-of-central-nervous-system-opportunistic-infections-and-mortality-in-hiv-infected-adults-in-central-china
#20
Rongrong Yang, Hong Zhang, Yong Xiong, Xien Gui, Yongxi Zhang, Liping Deng, Shicheng Gao, Mingqi Luo, Wei Hou, Deyin Guo
BACKGROUND: CSF PCR is the standard diagnostic technique used in resource-rich settings to detect pathogens of the CNS infection. However, it is not currently used for routine CSF testing in China. Knowledge of CNS opportunistic infections among people living with HIV in China is limited. METHODS: Intensive cerebrospiral fluid (CSF) testing was performed to evaluate for bacterial, viral and fungal etiologies. Pathogen-specific primers were used to detect DNA from cytomegalovirus (CMV), herpes simplex virus (HSV), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6) and John Cunningham virus (JCV) via real-time polymerase chain reaction (PCR)...
2017: AIDS Research and Therapy
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