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https://www.readbyqxmd.com/read/29053085/rifampicin-resistance-and-multidrug-resistant-tuberculosis-detection-using-xpert-mtb-rif-in-wuhan-china-a-retrospective-study
#1
Hai Huang, Yanlin Zhang, Sheng Li, Jun Wang, Jun Chen, Zhiyun Pan, Hui Gan
BACKGROUND: The Xpert MTB/RIF test (Cepheid, Sunnyvale, CA) can simultaneously detect the Mycobacterium tuberculosis (MTB) complex DNA and rifampicin (RFP) resistance and can rapidly determine RFP resistance and predict multidrug-resistant tuberculosis (MDR-TB). In this study, we analyzed clinical examination results of a hospital specializing in TB treatment in Wuhan, Hubei, China, and examined the use of traditional culture and drug-sensitive test (DST) results as a gold standard to assess the diagnosis value of the Xpert MTB/RIF test in RFP resistance and MDR-TB...
October 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29033301/characterization-of-gyra-and-gyrb-mutations-associated-with-fluoroquinolone-resistance-in-mycobacterium-tuberculosis-isolates-from-morocco
#2
Imane Chaoui, Amal Oudghiri, Mohammed El Mzibri
BACKGROUND: Fluoroquinolones (FQs) are the cornerstone for treatment of drug-resistant TB, there are the most effective second-line anti-mycobacterial drugs and are recommended for the treatment of MDR-TB. However, it's widely accepted that FQs resistance is high among MDR-TB. Thus, characterization of mutations conferring resistance to FQs will be of a great interest for an effective and efficient management of TB resistance in Morocco. METHODS: A laboratory collection of 30 MTB isolates already characterized as phenotypically and genotypically MDR and 20 pan-susceptible isolates randomly selected were enrolled in this retrospective study...
October 12, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29023060/polymorphism-of-glutamate-cysteine-ligase-subunit-catalytic-gclc-gene-in-pulmonary-tuberculosis-patients
#3
Ari Yuniastuti, R Susanti, Dewi Mustikaningtyas
BACKGROUND AND OBJECTIVE: The biomarker of oxidative stress in pulmonary tuberculosis patients has not been found. Oxidative stress occurs due to the low level of antioxidants. Single nucleotide polymorphism of glutamate-cysteine ligase subunit catalytic (GCLC) gene namely -129C/T GCLC has been reported to have an association with a risk factor of oxidative stress' susceptibility. The Objective of this study was to determine the GCLC polymorphism in pulmonary tuberculosis (TB) patient...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29019234/a-sensitive-electrochemical-aptasensor-for-thrombin-detection-based-on-electroactive-co-based-metal-organic-frameworks-with-target-triggering-nesa-strategy
#4
Xia Yang, Jiajia Lv, Zhehan Yang, Ruo Yuan, Ya-Qin Chai
In this work, an improved target-triggering nicking enzyme signaling amplification (NESA) strategy as signal enhancer has been fabricated to obtain a sensitive electrochemical thrombin (TB) biosensor combined with PtPd NPs decorated electroactive Co-based metal-organic frameworks (Co-MOFs/PtPdNPs) as a redox mediator. Traditionally, in the NESA strategy, only one of the output double strands DNA is available in the next cycle. However, in this work, all of the output DNA involved in the improved NESA strategies could be further employed, resulting in high utilization of output DNA, which further enhanced signal amplification and sensitivity of the biosensor...
October 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28988027/gelred-g3t-5-tb-3-hybrid-a-novel-label-free-ratiometric-fluorescent-probe-for-h2o2-and-oxidase-based-visual-biosensing
#5
Jie-Qiong Chen, Shi-Fan Xue, Zi-Han Chen, Shengqiang Zhang, Guoyue Shi, Min Zhang
A novel label-free ratiometric fluorescent probe is developed for the detection of H2O2 based on GelRed/[G3T]5/Tb(3+) hybrid, in which GelRed (a nucleic acid dye) intercalated into the designed single-stranded DNA [G3T]5 (i.e. GelRed/[G3T]5) acts as a stable build-in reference with red emission and Tb(3+) sensitized by [G3T]5 (i.e. [G3T]5/Tb(3+)) as a sensitive response signal with green fluorescence. With the successive addition of Hg(2+) and cysteine (Cys) to GelRed/[G3T]5/Tb(3+), the fluorescence of [G3T]5/Tb(3+) can be effectively quenched and recovered respectively, while the fluorescence of GelRed/[G3T]5 remains unchangeable...
September 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28966626/natural-agrobacterium-transformants-recent-results-and-some-theoretical-considerations
#6
REVIEW
Ke Chen, Léon Otten
Agrobacterium rhizogenes causes hairy root growth on a large number of plant species. It does so by transferring specific DNA fragments (T-DNA) from its root-inducing plasmid (pRi) into plant cells. Expression of T-DNA genes leads to abnormal root growth and production of specific metabolites (opines) which are taken up by the bacterium and used for its growth. Recent work has shown that several Nicotiana, Linaria, and Ipomoea species contain T-DNA genes from A. rhizogenes in their genomes. Plants carrying such T-DNAs (called cellular T-DNA or cT-DNA) can be considered as natural transformants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28959248/rifabutin-resistance-associated-with-double-mutations-in-rpob-gene-in-mycobacterium-tuberculosis-isolates
#7
Wei Jing, Yu Pang, Zhaojing Zong, Jing Wang, Ru Guo, Fengmin Huo, Guanglu Jiang, Yifeng Ma, Hairong Huang, Naihui Chu
The objective of this study was to investigate the cross-resistance between rifampin (RIF) and rifabutin (RFB) among clinical Mycobacterium tuberculosis (MTB) isolates, and the correlations between specific rpoB mutations and the minimum inhibitory concentrations (MICs) of RIF and RFB. A total of 256 RIF-resistant isolates were included from the National Tuberculosis Clinical Laboratory in China. The MICs of MTB isolates against RIF and RFB were determined by using a microplate alamarBlue assay. In addition, all the MTB isolates were sequenced for mutations in rpoB gene...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28957391/real-time-pcr-in-detection-and-quantitation-of-leishmania-donovani-for-the-diagnosis-of-visceral-leishmaniasis-patients-and-the-monitoring-of-their-response-to-treatment
#8
Faria Hossain, Prakash Ghosh, Md Anik Ashfaq Khan, Malcolm S Duthie, Aarthy C Vallur, Alessandro Picone, Randall F Howard, Steven G Reed, Dinesh Mondal
Sustained elimination of Visceral Leishmaniasis (VL) requires the reduction and control of parasite reservoirs to minimize the transmission of Leishmania donovani infection. A simple, reproducible and definitive diagnostic procedure is therefore indispensable for the early and accurate detection of parasites in VL, Relapsed VL (RVL) and Post Kala-azar Dermal Leishmaniasis (PKDL) patients, all of whom are potential reservoirs of Leishmania parasites. To overcome the limitations of current diagnostic approaches, a novel quantitative real-time polymerase chain reaction (qPCR) method based on Taqman chemistry was devised for the detection and quantification of L...
2017: PloS One
https://www.readbyqxmd.com/read/28951586/anti-mycobacterial-activity-correlates-with-altered-dna-methylation-pattern-in-immune-cells-from-bcg-vaccinated-subjects
#9
Deepti Verma, Venkata Ramanarao Parasa, Johanna Raffetseder, Mihaela Martis, Ratnesh B Mehta, Mihai Netea, Maria Lerm
The reason for the largely variable protective effect against TB of the vaccine Bacille Calmette-Guerin (BCG) is not understood. In this study, we investigated whether epigenetic mechanisms are involved in the response of immune cells to the BCG vaccine. We isolated peripheral blood mononuclear cells (PBMCs) from BCG-vaccinated subjects and performed global DNA methylation analysis in combination with functional assays representative of innate immunity against Mycobacterium tuberculosis infection. Enhanced containment of replication was observed in monocyte-derived macrophages from a sub-group of BCG-vaccinated individuals (identified as 'responders')...
September 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28941991/conservation-in-gene-encoding-mycobacterium-tuberculosis-antigen-rv2660-and-a-high-predicted-population-coverage-of-h56-multistage-vaccine-in-south-africa
#10
Angy P Perez-Martinez, Edison Ong, Lixin Zhang, Carl F Marrs, Yongqun He, Zhenhua Yang
H56/AERAS-456+IC31 (H56), composed of two early secretion proteins, Ag85B and ESAT-6, and a latency associated protein, Rv2660, and the IC31 Intercell adjuvant, is a new fusion subunit vaccine candidate designed to induce immunity against both new infection and reactivation of latent tuberculosis infection. Efficacy of subunit vaccines may be affected by the diversity of vaccine antigens among clinical strains and the extent of recognition by the diverse HLA molecules in the recipient population. Although a previous study showed the conservative nature of Ag85B- and ESAT-6-encoding genes, genetic diversity of Rv2660c that encodes RV2660 is largely unknown...
September 21, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28935157/thermal-lysis-and-isothermal-amplification-of-mycobacterium-tuberculosis-h37rv-in-one-tube
#11
Prasad Shetty, Dipayan Ghosh, Debjani Paul
Tuberculosis (TB) is a leading cause of high mortality rates in developing countries. Sample preparation is one of the major challenges in developing an inexpensive point-of-care device for rapid and confirmed detection of tuberculosis. Existing chemical and mechanical lysis methods are unsuitable for field applications, as they require intermediate wash steps, manual intervention or separate lysis equipment. We report a one-step reaction protocol (65°C and 60min) for the H37Rv strain of Mycobacterium tuberculosis that (i) completely disinfects the mycobacteria culture, (ii) lyses the cells and (iii) performs helicase dependent amplification on the extracted DNA...
September 19, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28901134/diagnosis-of-tuberculosis-using-colorimetric-gold-nanoparticles-on-a-paper-based-analytical-device
#12
Tsung-Ting Tsai, Chia-Yu Huang, Chung-An Chen, Shu-Wei Shen, Mei-Chia Wang, Chao-Min Cheng, Chien-Fu Chen
We have developed a colorimetric sensing strategy employing gold nanoparticles and a paper-based analytical platform for the diagnosis of tuberculosis (TB). By utilizing the surface plasmon resonance effect, we were able to monitor changes in the color of a gold nanoparticle colloid based on the effects of single-stranded DNA probe molecules hybridizing with targeted double-stranded TB DNA. The hybridization event changes the surface charge density of the nanoparticles, causing them to aggregate to various degrees, which modifies the color of the solution in a manner that can be readily measured to determine the concentration of the targeted DNA analyte...
September 22, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28900077/-the-spectrum-of-mutations-in-genes-associated-with-resistance-to-rifampicin-isoniazid-and-fluoroquinolones-in-the-clinical-strains-of-m-tuberculosis-reflects-the-transmissibility-of-mutant-clones
#13
A Ergeshov, S N Andreevskaya, E E Larionova, T G Smirnova, L N Chernousova
To study the transmissibility of drug resistant mutant clones, M. tuberculosis samples were isolated from the patients of the clinical department and the polyclinic of the Central TB Research Institute (n = 1455) for 2011-2014. A number of clones were phenotypically resistant to rifampicin (n = 829), isoniazid (n = 968), and fluoroquinolones (n = 220). We have detected 21 resistance-associated variants in eight codons of rpoB, six variants in three codons of katG, three variants in two positions of inhA, four variants in four positions of ahpC, and nine variants in five codons of gyrA, which were represented in the analyzed samples with varied frequencies...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28883399/antibiotic-treatment-for-tuberculosis-induces-a-profound-dysbiosis-of-the-microbiome-that-persists-long-after-therapy-is-completed
#14
Matthew F Wipperman, Daniel W Fitzgerald, Marc Antoine Jean Juste, Ying Taur, Sivaranjani Namasivayam, Alan Sher, James M Bean, Vanni Bucci, Michael S Glickman
Mycobacterium tuberculosis, the cause of Tuberculosis (TB), infects one third of the world's population and causes substantial mortality worldwide. In its shortest format, treatment of TB requires six months of multidrug therapy with a mixture of broad spectrum and mycobacterial specific antibiotics, and treatment of multidrug resistant TB is longer. The widespread use of this regimen makes this one of the largest exposures of humans to antimicrobials, yet the effects of TB treatment on intestinal microbiome composition and long-term stability are unknown...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28868128/design-of-peptides-interfering-with-iron-dependent-regulator-ider-and-evaluation-of-mycobacterium-tuberculosis-growth-inhibition
#15
Himen Salimizand, Saeid Amel Jamehdar, Leila Babaei Nik, Hamid Sadeghian
OBJECTIVES: Tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (Mtb), stayed a global health thread with high mortality rate. Since TB has a long-term treatment, it leads high risk of drug resistant development, and there is an urgent to find new drugs. The aim of this study was designing new inhibitors for a new drug target, iron dependent regulator, IdeR. MATERIALS AND METHODS: Based on the interaction most populated amino acids of IdeR to the related gene operators, 50 short peptides were modeled...
June 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28861054/screening-of-tb-actives-for-activity-against-nontuberculous-mycobacteria-delivers-high-hit-rates
#16
Jian Liang Low, Mu-Lu Wu, Dinah Binte Aziz, Benoît Laleu, Thomas Dick
The prevalence of lung disease due to infections with nontuberculous mycobacteria (NTM) has been increasing and surpassed tuberculosis (TB) in some countries. Treatment outcomes are often unsatisfactory, highlighting an urgent need for new anti-NTM medications. Although NTM in general do not respond well to TB specific drugs, the similarities between NTM and Mycobacterium tuberculosis at the molecular and cell structural level suggest that compound libraries active against TB could be leveraged for NTM drug discovery...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28851844/the-new-xpert-mtb-rif-ultra-improving-detection-of-mycobacterium-tuberculosis-and-resistance-to-rifampin-in-an-assay-suitable-for-point-of-care-testing
#17
Soumitesh Chakravorty, Ann Marie Simmons, Mazhgan Rowneki, Heta Parmar, Yuan Cao, Jamie Ryan, Padmapriya P Banada, Srinidhi Deshpande, Shubhada Shenai, Alexander Gall, Jennifer Glass, Barry Krieswirth, Samuel G Schumacher, Pamela Nabeta, Nestani Tukvadze, Camilla Rodrigues, Alena Skrahina, Elisa Tagliani, Daniela M Cirillo, Amy Davidow, Claudia M Denkinger, David Persing, Robert Kwiatkowski, Martin Jones, David Alland
The Xpert MTB/RIF assay (Xpert) is a rapid test for tuberculosis (TB) and rifampin resistance (RIF-R) suitable for point-of-care testing. However, it has decreased sensitivity in smear-negative sputum, and false identification of RIF-R occasionally occurs. We developed the Xpert MTB/RIF Ultra assay (Ultra) to improve performance. Ultra and Xpert limits of detection (LOD), dynamic ranges, and RIF-R rpoB mutation detection were tested on Mycobacterium tuberculosis DNA or sputum samples spiked with known numbers of M...
August 29, 2017: MBio
https://www.readbyqxmd.com/read/28842221/rapid-and-visual-detection-of-mycobacterium-tuberculosis-complex-using-recombinase-polymerase-amplification-combined-with-lateral-flow-strips
#18
Qinglin Ma, Houming Liu, Feidi Ye, Guangxin Xiang, Wanshui Shan, Wanli Xing
To definitively diagnose active pulmonary Tuberculosis (TB), Mycobacterium tuberculosis complex (MTBC) bacilli must be identified within clinical specimens from patients. In this study, we introduced a rapid and visual detection method of MTBC using recombinase polymerase amplification (RPA) combined with lateral flow (LF) strips. The LF-RPA assay, read results with naked eyes, could detect as few as 5 genome copies of M. tuberculosis H37Rv (ATCC 27294) per reaction and had no cross-reactions with other control bacteria even using excessive amount of template DNA...
August 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28818102/field-evaluation-of-the-efficacy-of-mycobacterium-bovis-bcg-vaccine-against-tuberculosis-in-goats
#19
Enric Vidal, Claudia Arrieta-Villegas, Miriam Grasa, Irene Mercader, Mariano Domingo, Bernat Pérez de Val
BACKGROUND: Control of animal tuberculosis (TB) through vaccination has emerged as a long-term strategy to complement test and slaughter control strategy. A pilot trial under field conditions was conducted in a goat herd with high TB prevalence to assess the efficacy of the Mycobacterium bovis BCG vaccine. RESULTS: Twenty-three goat kids vaccinated with BCG and other 22 unvaccinated control kids were euthanized at 18 months post-vaccination. Gross pathological and histopathological examination of target tissues was performed for detection of tuberculous lesions and assessment of vaccine efficacy...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28817723/sequence-analysis-of-the-rifampicin-resistance-determining-region-rrdr-of-rpob-gene-in-multidrug-resistance-confirmed-and-newly-diagnosed-tuberculosis-patients-of-punjab-pakistan
#20
Salma Hameed, Kartyk Moganeradj, Nasir Mahmood, Timothy D McHugh, Muhammad Nawaz Chaudhry, Catherine Arnold
Molecular screening of new patients suspected for TB could help in the effective control of TB in Pakistan as it is a high TB burden country. It will be informative to understand the prevalence of multi drug resistance for a better drug regimen management in this geographical area. The Rifampicin resistance determining region (RRDR) sequencing was used to identify mutations associated with drug resistance in DNA extracts from 130 known multidrug resistant (MDR) cultured strains and compared with mutations observed in DNA extracts directly from 86 sputum samples from consecutive newly diagnosed cases in Lahore, Pakistan...
2017: PloS One
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