keyword
MENU ▼
Read by QxMD icon Read
search

TB DNA

keyword
https://www.readbyqxmd.com/read/29216262/direct-detection-of-mycobacterium-tuberculosis-rifampin-resistance-in-bio-safe-stained-sputum-smears
#1
Surabhi Lavania, Divya Anthwal, Manpreet Bhalla, Nagendra Singh, Sagarika Haldar, Jaya Sivaswami Tyagi
Direct smear microscopy of sputum forms the mainstay of TB diagnosis in resource-limited settings. Stained sputum smear slides can serve as a ready-made resource to transport sputum for molecular drug susceptibility testing. However, bio-safety is a major concern during transport of sputum/stained slides and for laboratory workers engaged in processing Mycobacterium tuberculosis infected sputum specimens. In this study, a bio-safe USP (Universal Sample Processing) concentration-based sputum processing method (Bio-safe method) was assessed on 87 M...
2017: PloS One
https://www.readbyqxmd.com/read/29204139/mycobacterium-tuberculosis-latent-antigen-rv2029c-from-the-multistage-dna-vaccine-a39-drives-th1-responses-via-tlr-mediated-macrophage-activation
#2
Haibo Su, Shengling Zhu, Lin Zhu, Cong Kong, Qi Huang, Zhi Zhang, Honghai Wang, Ying Xu
Targeting of Mycobacterium tuberculosis (MTB) latent antigens comprises a crucial strategy for the development of alternative tuberculosis (TB) vaccine(s) that protects against TB reactivation. Here, we generated a multistage DNA vaccine, A39, containing the early antigens Ag85A and Rv3425 as well as the latency-associated protein Rv2029c, which conferred protective immunity in a pre-exposure mouse model. Moreover, administration of the A39 vaccination after MTB exposure inhibited reactivation and resulted in significantly lower bacterial loads in the lungs and spleen of mice, compared to those in the control population...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29190812/diagnostic-performance-of-the-1-3-%C3%AE-d-glucan-assay-in-patients-with-pneumocystis-jirovecii-compared-with-those-with-candidiasis-aspergillosis-mucormycosis-and-tuberculosis-and-healthy-volunteers
#3
Hyo-Ju Son, Heungsup Sung, Se Yoon Park, Taeeun Kim, Hyun Jeong Lee, Sun-Mi Kim, Yong Pil Chong, Sang-Oh Lee, Sang-Ho Choi, Yang Soo Kim, Jun Hee Woo, Sung-Han Kim
BACKGROUND: Diagnosis of pneumocystis pneumonia (PCP) relies on microscopic visualization of P. jirovecii, or detection of Pneumocystis DNA in respiratory specimens, which involves invasive procedures such as bronchoalveolar lavage. The (1-3)-β-D-glucan (BG) assay has been proposed as a less invasive and less expensive diagnostic test to rule out PCP. We therefore compared blood levels of BG in patients with PCP with those of patients with candidemia, chronic disseminated candidiasis (CDC), invasive aspergillosis, mucormycosis, and tuberculosis and those of healthy volunteers...
2017: PloS One
https://www.readbyqxmd.com/read/29184888/targeting-dna-replication-and-repair-for-the-development-of-novel-therapeutics-against-tuberculosis
#4
REVIEW
Michael A Reiche, Digby F Warner, Valerie Mizrahi
Mycobacterium tuberculosis is the etiological agent of tuberculosis (TB), an infectious disease which results in approximately 10 million incident cases and 1.4 million deaths globally each year, making it the leading cause of mortality from infection. An effective frontline combination chemotherapy exists for TB; however, this regimen requires the administration of four drugs in a 2 month long intensive phase followed by a continuation phase of a further 4 months with two of the original drugs, and is only effective for the treatment of drug-sensitive TB...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/29171846/sm-iii-12-mcga-iii-shi-4-as-a-luminescent-probe-for-g-quadruplex-structures
#5
Ewa Rajczak, Vincent L Pecoraro, Bernard Juskowiak
G-quadruplexes (GQs), spatial assemblies of guanine-rich DNA strands, play an important role in the regulation of gene expression and chromosome stabilization. These structures are recognized to be useful in cancer therapies as the presence of multiple G-quadruplexes in a telomeric strand stops cancer cell proliferation. Metallacrowns of the type 12-MC-4 form planar structures that have remarkable similarity to G-tetrads in terms of dimension, shape and the ability to bind alkali metal and lanthanide cations in a central cavity...
November 24, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29166403/integration-of-molecular-typing-results-into-tuberculosis-surveillance-in-germany-a-pilot-study
#6
Marta Andrés, Elke Göhring-Zwacka, Lena Fiebig, Martin Priwitzer, Elvira Richter, Sabine Rüsch-Gerdes, Walter Haas, Stefan Niemann, Bonita Brodhun
An integrated molecular surveillance for tuberculosis (TB) improves the understanding of ongoing TB transmission by combining molecular typing and epidemiological data. However, the implementation of an integrated molecular surveillance for TB is complex and requires thoughtful consideration of feasibility, demand, public health benefits and legal issues. We aimed to pilot the integration of molecular typing results between 2008 and 2010 in the German Federal State of Baden-Württemberg (population 10.88 Million) as preparation for a nationwide implementation...
2017: PloS One
https://www.readbyqxmd.com/read/29165665/novel-structural-features-drive-dna-binding-properties-of-cmr-a-crp-family-protein-in-tb-complex-mycobacteria
#7
Sridevi Ranganathan, Jonah Cheung, Michael Cassidy, Christopher Ginter, Janice D Pata, Kathleen A McDonough
Mycobacterium tuberculosis (Mtb) encodes two CRP/FNR family transcription factors (TF) that contribute to virulence, Cmr (Rv1675c) and CRPMt (Rv3676). Prior studies identified distinct chromosomal binding profiles for each TF despite their recognizing overlapping DNA motifs. The present study shows that Cmr binding specificity is determined by discriminator nucleotides at motif positions 4 and 13. X-ray crystallography and targeted mutational analyses identified an arginine-rich loop that expands Cmr's DNA interactions beyond the classical helix-turn-helix contacts common to all CRP/FNR family members and facilitates binding to imperfect DNA sequences...
November 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29154992/structural-and-biophysical-characterization-of-rv3716c-a-hypothetical-protein-from-mycobacterium-tuberculosis
#8
A Gopalan, G Deka, M Prabhavathi, H S Savithri, M R N Murthy, A Raja
Latent tuberculosis (TB) is the main hurdle in reaching the goal of "Stop TB 2050". Tuberculin skin and Interferon-gamma release assay tests used currently for the diagnosis of TB infection cannot distinguish between active disease and latent tuberculosis infection (LTBI) and hence new and sensitive protein markers need to be identified for the diagnosis. A protein Rv3716c from Mycobacterium tuberculosis (MtbRv3716c) has been identified as a potential surrogate marker for the diagnosis of LTBI. Here, we present characterization of MtbRv3716c (∼13 kDa) using both biophysical and X-Ray crystallographic methods...
November 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29147487/a-study-on-the-immune-response-induced-by-a-dna-vaccine-encoding-mtb32c-hbha-antigen-of-mycobacterium-tuberculosis
#9
Roghayeh Teimourpour, Hadi Peeridogaheh, Amir Teimourpour, Mohsen Arzanlou, Zahra Meshkat
Objectives: Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. Materials and Methods: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29143676/the-future-of-viral-hepatitis-testing-innovations-in-testing-technologies-and-approaches
#10
REVIEW
Rosanna W Peeling, Debrah I Boeras, Francesco Marinucci, Philippa Easterbrook
A large burden of undiagnosed hepatitis virus cases remains globally. Despite the 257 million people living with chronic hepatitis B virus infection, and 71 million with chronic viraemic HCV infection, most people with hepatitis remain unaware of their infection. Advances in rapid detection technology have created new opportunities for enhancing access to testing and care, as well as monitoring of treatment. This article examines a range of other technological innovations that can be leveraged to provide more affordable and simplified approaches to testing for HBV and HCV infection and monitoring of treatment response...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29142798/verification-of-diagnosis-in-tuberculosis-a-case-report-and-discussion
#11
Amanda M Dave, Abed Adelrahman, Vishist Mehta, Stephen Cavalieri, Renuga Vivekanadan
Tuberculosis (TB), caused by strains of Mycobacterium tuberculosis complex (M. tuberculosis), is a pulmonary infection that is spread by airborne droplet transmission. The development and spread of drug-resistant strains of M. tuberculosisgreatly jeopardize TB control efforts. We report the case of a previously healthy 43-year-old male, visiting from China, who presented to the emergency department complaining of hemoptysis of 10 days' duration. Cultures were positive for acid fast bacteria and negative for fungi...
September 3, 2017: Curēus
https://www.readbyqxmd.com/read/29138284/rapid-diagnosis-of-pulmonary-tuberculosis-and-detection-of-drug-resistance-by-combined-simultaneous-amplification-testing-and-reverse-dot-blot
#12
Yiwen Chen, Lahong Zhang, Liquan Hong, Xian Luo, Juping Chen, Leiming Tang, Jiahuan Chen, Xia Liu, Zhaojun Chen
AIMS: Making a correct and rapid diagnosis is essential for managing pulmonary tuberculosis (PTB), particularly multidrug-resistant tuberculosis. We aimed to evaluate the efficacy of the combination of simultaneous amplification testing (SAT) and reverse dot blot (RDB) for the rapid detection of Mycobacterium tuberculosis (MTB) and drug-resistant mutants in respiratory samples. METHODS: 225 suspected PTB and 32 non-TB pulmonary disease samples were collected. All sputum samples were sent for acid-fast bacilli smear, SAT, culture and drug susceptibility testing (DST) by the BACTEC(TM) MGIT(TM) 960 system...
November 14, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29137710/loop-mediated-isothermal-amplification-using-self-avoiding-molecular-recognition-systems-and-antarctic-thermal-sensitive-uracil-dna-glycosylase-for-detection-of-nucleic-acid-with-prevention-of-carryover-contamination
#13
Yi Wang, Dongxin Liu, Jianping Deng, Yan Wang, Jianguo Xu, Changyun Ye
Loop-mediated isothermal amplification (LAMP) is the most popular technique to amplify nucleic acid sequence without the use of temperature cycling. However, LAMP is often confounded by false-positive results, arising from interactions between (hetero-dimer) or within (self-dimerization) primers, off-target hybrids and carryover contaminants. Here, we devised a new LAMP technique that is self-avoiding molecular recognition system (SAMRS) components and antarctic thermal sensitive uracil-DNA-glycosylase (AUDG) enzyme-assisted, termed AUDG-SAMRS-LAMP...
December 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29132287/a-qualitative-analysis-of-the-barriers-to-antiretroviral-therapy-initiation-among-children-2-to-18-months-of-age-in-swaziland
#14
Charisse V Ahmed, Pauline Jolly, Luz Padilla, Musa Malinga, Chantal Harris, Nobuhle Mthethwa, Inessa Ba, Amy Styles, Sarah Perry, Raina Brooks, Florence Naluyinda-Kitabire, Makhosini Mamba, Peter Preko
HIV/AIDS remains one of the leading causes of death among children under 5 years old in Swaziland. Although studies have shown that early initiation of infants and children diagnosed with HIV on antiretroviral therapy (ART) significantly reduces mortality, many children do not initiate ART until the later stages of disease. This study was designed to collect qualitative data from mothers and caregivers of HIV-positive children to identify the barriers to ART initiation. Focus group discussion (FGD) sessions were conducted in siSwati between July and September 2014 among caregivers of aged children 2-18 months in Swaziland who did or did not initiate ART between January 2011 and December 2012 after HIV DNA PCR-positive diagnosis of the infants...
December 2017: African Journal of AIDS Research: AJAR
https://www.readbyqxmd.com/read/29129484/detection-of-resistance-to-fluoroquinolones-and-injectable-drugs-among-antituberculosis-drugs-by-allele-specific-primer-extension-on-a-microsphere-based-platform
#15
Kyungjong Kim, Jeong Seong Yang, Hee Baeg Choi, Seung Heon Lee
Molecular drug susceptibility testing (DST) for antituberculosis drugs is important for improving the efficacy of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) treatment. In this study, we developed a molecular high-throughput assay system based on allele-specific primer extension (ASPE) and MagPlex-TAG microspheres, referred to here as TAG-ASPE, which can detect mutations related to resistance to injectable second-line drugs and fluoroquinolones. Target genes were amplified by multiplex PCR using DNA from H37Rv and 190 clinical Mycobacterium tuberculosis strains and extended by ASPE using 22 ASPE primers...
November 9, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29124333/assessment-of-hepatotoxicity-of-first-line-anti-tuberculosis-drugs-on-wistar-rats
#16
Radhika Sharma, Ramneek Kaur, Manishi Mukesh, Vijay L Sharma
Adverse drug reactions are inevitable risk factors associated with use of modern medicines. First-line anti-tuberculosis drugs contribute to diverse pathological complications, and hepatotoxicity is one of them. This study investigated the effects of anti-TB drugs in combination (rifampicin [RIF] + isoniazid [INH] + pyrazinamide [PZA]) on Wistar rats. Rats were grouped as control group (saline), toxicant group that was given (30.85 mg/kg b.wt., INH + 61.7 mg/kg b.wt., RIF + 132.65 mg/kg b.wt. PZA in dosage extrapolated from dose that is used in human)...
November 9, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/29122502/clinical-and-pathologic-analyses-of-tuberculosis-in-the-oral-cavity-report-of-11-cases
#17
Wu-Tong Ju, Yong Fu, Ying Liu, Yi-Ran Tan, Min-Jun Dong, Li-Zhen Wang, Jiang Li, Lai-Ping Zhong
OBJECTIVE: The aim of this study was to analyze tuberculosis (TB) in the oral cavity according to clinical appearance, clinical differential diagnosis, treatment, and outcome. STUDY DESIGN: We enrolled 11 patients with TB in the oral cavity between November 2012 and November 2016. Glossal lymphoid TB was excluded. Clinical symptoms, auxiliary examinations, treatments, and outcomes were recorded and analyzed. RESULTS: The study population comprised 6 men and 5 women, with a mean age of 59 years...
September 28, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/29122403/validation-of-simple-and-cost-effective-stains-to-assess-acrosomal-status-dna-damage-and-mitochondrial-activity-in-rooster-spermatozoa
#18
Bruno R Rui, Daniel S R Angrimani, João Diego A Losano, Luana de Cássia Bicudo, Marcílio Nichi, Ricardo J G Pereira
Several methods have been developed to evaluate spermatozoa function in birds but many of these are sometimes complicated, costly and not applicable to field studies (i.e., performed within poultry breeding facilities). The objective was, therefore, to validate efficient, practical and inexpensive procedures to determine DNA fragmentation, acrosomal integrity, and mitochondrial activity in poultry spermatozoa. Initially, ejaculates were individually diluted and divided into control (4°C, 4h) and UV-irradiated aliquots (room temperature, 4h), and then samples containing different percentages of DNA-damaged spermatozoa (0%, 25%, 50%, 75% and 100%) were subjected to Toluidine Blue (TB) and Sperm Chromatin Dispersion assessments (SCD)...
December 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29119205/secretome-profile-analysis-of-multidrug-resistant-monodrug-resistant-and-drug-susceptible-mycobacterium-tuberculosis
#19
Chanyanuch Putim, Narumon Phaonakrop, Janthima Jaresitthikunchai, Ratikorn Gamngoen, Khajornsak Tragoolpua, Sorasak Intorasoot, Usanee Anukool, Chayada Sitthidet Tharincharoen, Ponrut Phunpae, Chatchai Tayapiwatana, Watchara Kasinrerk, Sittiruk Roytrakul, Bordin Butr-Indr
The emergence of drug-resistant tuberculosis has generated great concern in the control of tuberculosis and HIV/TB patients have established severe complications that are difficult to treat. Although, the gold standard of drug-susceptibility testing is highly accurate and efficient, it is time-consuming. Diagnostic biomarkers are, therefore, necessary in discriminating between infection from drug-resistant and drug-susceptible strains. One strategy that aids to effectively control tuberculosis is understanding the function of secreting proteins that mycobacteria use to manipulate the host cellular defenses...
November 8, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29118176/highly-sensitive-detection-of-isoniazid-heteroresistance-in-mycobacterium-tuberculosis-by-deepmelt-assay
#20
Bin Liang, Yaoju Tan, Zi Li, Xueshan Tian, Chen Du, Hui Li, Guoli Li, Xiangyang Yao, Zhongan Wang, Ye Xu, Qingge Li
Detection of heteroresistance of Mycobacterium tuberculosis (MTB) remains challenging using current genotypic drug susceptibility testing methods. Here, we described a melting curve analysis-based approach, termed DeepMelt, that can detect less abundant mutants through selective clamping of the wild-type in mixed populations. Singleplex DeepMelt assay detected 0.01% katG S315T in 10(5) MTB genomes/μL. Multiplex DeepMelt TB/INH detected 1% of mutant species in the four loci associated with isoniazid resistance in 10(4) MTB genomes/μL...
November 8, 2017: Journal of Clinical Microbiology
keyword
keyword
43551
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"