Kazuhiro Sumida, Tomomitsu Doi, Kunie Obayashi, Yosuke Chiba, Shohei Nagasaka, Noriyoshi Ogino, Koichiro Miyagawa, Ryoko Baba, Hiroyuki Morimoto, Hideki Hara, Takeshi Terabayashi, Toshimasa Ishizaki, Masaru Harada, Motoyoshi Endo
In addition to its role in pyroptosis and inflammatory cytokine maturation, caspase-4 (CASP4) also contributes to the fusion of phagosomes with lysosomes and cell migration. However, its role in cell division remains elusive. In this study, we demonstrate that CASP4 is indispensable for proper cell division in epithelial cells. Knockout of CASP4 (CASP4 KO) in HepG2 cells led to delayed cell proliferation, increased cell size, and increased multinucleation. In mitosis, CASP4 KO cells showed multipolar spindles, asymmetric spindle positioning, and chromosome segregation errors, ultimately increasing DNA content and chromosome number...
December 19, 2023: Biochemical and Biophysical Research Communications