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https://www.readbyqxmd.com/read/29925053/seawater-acidification-aggravated-cadmium-toxicity-in-the-oyster-crassostrea-gigas-metal-bioaccumulation-subcellular-distribution-and-multiple-physiological-responses
#1
Ruiwen Cao, Yongliang Liu, Qing Wang, Zhijun Dong, Dinglong Yang, Hui Liu, Wen Ran, Yi Qu, Jianmin Zhao
Mounting evidence has demonstrated the combined effects of ocean acidification (OA) and other environmental stressors on marine organisms. Although metal pollution is widely distributed in coasts and estuaries, the combined effects of OA and metal pollution have received little attention until recent years. In this study, the accumulation and subcellular distribution of cadmium (Cd) and the physiological responses of the oyster Crassostrea gigas were investigated after 31 days of exposure to OA and Cd, either alone or in combination...
June 17, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29920567/factors-affecting-infection-of-corals-and-larval-oysters-by-vibrio-coralliilyticus
#2
Blake Ushijima, Gary P Richards, Michael A Watson, Carla B Schubiger, Claudia C Häse
The bacterium Vibrio coralliilyticus can threaten vital reef ecosystems by causing disease in a variety of coral genera, and, for some strains, increases in virulence at elevated water temperatures. In addition, strains of V. coralliilyticus (formally identified as V. tubiashii) have been implicated in mass mortalities of shellfish larvae causing significant economic losses to the shellfish industry. Recently, strain BAA-450, a coral pathogen, was demonstrated to be virulent towards larval Pacific oysters (Crassostrea gigas)...
2018: PloS One
https://www.readbyqxmd.com/read/29915695/long-term-experimental-in-situ-farming-of-crambe-crambe-demospongiae-poecilosclerida
#3
Andrea Padiglia, Fabio D Ledda, Bachisio M Padedda, Roberto Pronzato, Renata Manconi
Background: The marine sponge Crambe crambe was chosen as an experimental model of sustainable shallow-water mariculture in the Sardinian Sea (Western Mediterranean) to provide biomass with high potential in applied research. Methods: Explants were cultured in four long-term experiments (19 and 31 months at ca. 2.5 m depth), to determine the suitability of new culture techniques by testing substrata and seeding time (season), and monitoring survival and growth. Explants were excised and grown in an experimental plant close to the wild donor sponge population...
2018: PeerJ
https://www.readbyqxmd.com/read/29912963/optimal-age-of-the-donor-graft-tissue-in-relation-to-cultured-pearl-phenotypes-in-the-mollusc-pinctada-margaritifera
#4
Carole Blay, Serge Planes, Chin-Long Ky
Ageing is defined as the progressive decline in tissue and organ functions over time. This study aims to evaluate the ageing effect on cultured pearl quality phenotypes (including size and quality traits) in the graft-recipient animal model: Pinctada margaritifera. For this, eight uniform grafting experiments were designed using two hatchery-produced pearl oyster families as donors, which were followed through time, between 7 and 30 months in age. For each age category, 20 donors were studied for each culture site giving a total of 2400 grafted oysters...
2018: PloS One
https://www.readbyqxmd.com/read/29910029/comparative-sensitivity-of-crassostrea-angulata-and-crassostrea-gigas-embryo-larval-development-to-as-under-varying-salinity-and-temperature
#5
Anthony Moreira, Etelvina Figueira, Giovanni Libralato, Amadeu M V M Soares, Marco Guida, Rosa Freitas
Oysters are a diverse group of marine bivalves that inhabit coastal systems of the world's oceans, providing a variety of ecosystem services, and represent a major socioeconomic resource. However, oyster reefs have become inevitably impacted from habitat destruction, overfishing, pollution and disease outbreaks that have pushed these structures to the break of extinction. In addition, the increased frequency of climate change related events promise to further challenge oyster species survival worldwide. Oysters' early embryonic development is likely the most vulnerable stage to climate change related stressors (e...
June 7, 2018: Marine Environmental Research
https://www.readbyqxmd.com/read/29906693/proteomic-response-of-gill-microsomes-of-crassostrea-brasiliana-exposed-to-diesel-fuel-water-accommodated-fraction
#6
Gabrielle do Amaral E Silva Müller, Karim Hahn Lüchmann, Guilherme Razzera, Guilherme Toledo-Silva, Maria João Bebianno, Maria Risoleta Freire Marques, Afonso Celso Dias Bainy
Diesel fuel water-accommodated fraction (diesel-WAF) is a complex mixture of organic compounds that may cause harmful effects to marine invertebrates. Expression of microsomal proteins can be changed by oil exposure, causing functional alterations in endoplasmic reticulum (ER). The aim of this study was to investigate changes in protein expression signatures in microsomes of oysterl Crassostrea brasiliana (=C.gasar) gill after exposure to 10% diesel-WAF for 24 and 72 h. Protein expression signatures of gills of oysters exposed to diesel-WAF were compared to those of unexposed oysters using two-dimensional electrophoresis (2-DE) to identify differentially expressed proteins...
June 6, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29903822/genome-sequence-of-a-canadian-vibrio-parahaemolyticus-isolate-with-unique-mobilizing-capacity
#7
Audrey Bioteau, Kévin Huguet, Vincent Burrus, Swapan Banerjee
Vibrio parahaemolyticus is a clinically significant marine bacterium implicated in gastroenteritis among consumers of raw or undercooked seafood. This report presents the whole-genome sequence of a unique strain of V. parahaemolyticus isolated from oysters harvested in Canada.
June 14, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29902761/cellular-responses-of-pacific-oyster-crassostrea-gigas-gametes-exposed-in-vitro-to-polystyrene-nanoparticles
#8
Carmen González-Fernández, Kevin Tallec, Nelly Le Goïc, Christophe Lambert, Philippe Soudant, Arnaud Huvet, Marc Suquet, Mathieu Berchel, Ika Paul-Pont
While the detection and quantification of nano-sized plastic in the environment remains a challenge, the growing number of polymer applications mean that we can expect an increase in the release of nanoplastics into the environment by indirect outputs. Today, very little is known about the impact of nano-sized plastics on marine organisms. Thus, the objective of this study was to investigate the toxicity of polystyrene nanoplastics (NPs) on oyster (Crassostrea gigas) gametes. Spermatozoa and oocytes were exposed to four NPs concentrations ranging from 0...
June 6, 2018: Chemosphere
https://www.readbyqxmd.com/read/29902509/design-and-evaluation-of-nested-pcr-primers-for-specific-detection-of-genogroup-i-noroviruses-in-oysters
#9
Ping Guo, Yongxin Yu, Yingjie Pan, Shuling Yan, Yongjie Wang
A pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters.
June 11, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29892231/the-molecular-mechanism-underlying-pro-apoptotic-role-of-hemocytes-specific-transcriptional-factor-lhx9-in-crassostrea-hongkongensis
#10
Yingli Zhou, Fan Mao, Zhiying He, Jun Li, Yuehuan Zhang, Zhiming Xiang, Shu Xiao, Haitao Ma, Yang Zhang, Ziniu Yu
Hemocytes are the central organ of immune defense against pathogens by means of inflammation, phagocytosis, and encapsulation in mollusks. The well-functioning of the host immune system relies on the hemocytes' task exertion and frequent renewal, but the underlying renewal mechanism remains elusive at the gene level. Here, we identified one transcription factor, LIM homeobox 9, in Crassostrea hongkongensis ( Ch Lhx9) that could be involved in hemocyte apoptosis or renewal. Ch Lhx9 contains a homeodomain and two LIM domains...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29886973/trophic-transfer-of-metals-in-a-seagrass-food-web-bioaccumulation-of-essential-and-non-essential-metals
#11
Larissa Schneider, William A Maher, Jaimie Potts, Anne M Taylor, Graeme E Batley, Frank Krikowa, Aaron Adamack, Anthony A Chariton, Bernd Gruber
Metal concentrations are reported for a seagrass ecosystem receiving industrial inputs. δ13 C and δ15 N isotope ratios were used to establish trophic links. Copper concentrations (dry mass) ranged from <0.01 μg/g in fish species to 570 μg/g (μ = 49 ± SD = 90 μg/g) in the oyster Saccostrea glomerata. Zinc concentrations ranged from 0.6 μg/g in the seagrass Zostera capricorni to 10,800 μg/g in the mud oyster Ostrea angasi (μ = 434 ± 1390 μg/g). Cadmium concentrations ranged from <0...
June 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29886933/tissue-specific-metabolic-responses-of-the-pearl-oyster-pinctada-martensii-exposed-to-benzo-a-pyrene
#12
Hao Chen, Xiaoping Diao, Hailong Zhou
Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that is well known for its teratogenic, mutagenic and carcinogenic effects. In this study, we applied metabolomics to investigate the tissue-specific metabolic responses of the Pinctada martensii digestive glands and gills after a short-duration exposure to BaP (1 μg/L and 10 μg/L). After 72 h of exposure to BaP, the majority of metabolite changes were related to osmolytes, energy metabolites, and amino acids. BaP (1 μg/L) accelerated energy deterioration and decreased osmotic regulation, while BaP (10 μg/L) disturbed energy metabolism and increased osmotic stress in the digestive glands...
June 2018: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/29886855/marteilia-refringens-and-marteilia-pararefringens-sp-nov-are-distinct-parasites-of-bivalves-and-have-different-european-distributions
#13
R Kerr, G M Ward, G D Stentiford, A Alfjorden, S Mortensen, J P Bignell, S W Feist, A Villalba, M J Carballal, A Cao, I Arzul, D Ryder, D Bass
Marteilia refringens causes marteiliosis in oysters, mussels and other bivalve molluscs. This parasite previously comprised two species, M. refringens and Marteilia maurini, which were synonymized in 2007 and subsequently referred to as M. refringens 'O-type' and 'M-type'. O-type has caused mass mortalities of the flat oyster Ostrea edulis. We used high throughput sequencing and histology to intensively screen flat oysters and mussels (Mytilus edulis) from the UK, Sweden and Norway for infection by both types and to generate multi-gene datasets to clarify their genetic distinctiveness...
June 11, 2018: Parasitology
https://www.readbyqxmd.com/read/29875442/population-resequencing-reveals-candidate-genes-associated-with-salinity-adaptation-of-the-pacific-oyster-crassostrea-gigas
#14
Zhicai She, Li Li, Jie Meng, Zhen Jia, Huayong Que, Guofan Zhang
The Pacific oyster Crassostrea gigas is an important cultivated shellfish. As a euryhaline species, it has evolved adaptive mechanisms responding to the complex and changeable intertidal environment that it inhabits. To investigate the genetic basis of this salinity adaptation mechanism, we conducted a genome-wide association study using phenotypically differentiated populations (hyposalinity and hypersalinity adaptation populations, and control population), and confirmed our results using an independent population, high-resolution melting, and mRNA expression analysis...
June 6, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29872651/ensuring-food-integrity-by-metrology-and-fair-data-principles
#15
Michael Rychlik, Giovanna Zappa, Larraitz Añorga, Nastasia Belc, Isabel Castanheira, Olivier F X Donard, Lenka Kouřimská, Nives Ogrinc, Marga C Ocké, Karl Presser, Claudia Zoani
Food integrity is a general term for sound, nutritive, healthy, tasty, safe, authentic, traceable, as well as ethically, safely, environment-friendly, and sustainably produced foods. In order to verify these properties, analytical methods with a higher degree of accuracy, sensitivity, standardization and harmonization and a harmonized system for their application in analytical laboratories are required. In this view, metrology offers the opportunity to achieve these goals. In this perspective article the current global challenges in food analysis and the principles of metrology to fill these gaps are presented...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29871790/electrospun-composite-plla-oyster-shell-scaffold-enhances-proliferation-and-osteogenic-differentiation-of-stem-cells
#16
Roghaieh Didekhani, Mahmoud Reza Sohrabi, Ehsan Seyedjafari, Masoud Soleimani, Hana Hanaee-Ahvaz
In bone tissue engineering, bioceramics are of the most widely used materials for treatment of bone defects clinically. The composites of bioceramic/polymer fibrous scaffolds have been designed and developed to fulfill the mechanical and biological requirements of the damaged tissue. In the present study, oyster shell (OS) as a bioceramic in combination with the biodegradable and biocompatible poly (l-lactide) has been used to prepare a new tissue-engineered composite. The morphology, porosity, water contact angle and mechanical properties of scaffolds were investigated...
June 2, 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29870546/bone-substitute-made-from-a-brazilian-oyster-shell-functions-as-a-fast-stimulator-for-bone-forming-cells-in-an-animal-model
#17
Ricardo Coringa, Eduardo Martins de Sousa, Juliana Nunes Botelho, Rafael Soares Diniz, Joicy Cortez de Sá, Maria Carmen Fontoura Nogueira da Cruz, Marco Aurelio Beninni Paschoal, Letícia Machado Gonçalves
Despite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeastern Brazil (Crassostrea rhizophora) has effects on bone development using an animal model. Oysters were collected from a biologically assisted vivarium, and their inner layer was used for preparing an EBS. Chemical and surface characterization of EBS was performed using Individually Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Scanning Electron Microscope (SEM), respectively...
2018: PloS One
https://www.readbyqxmd.com/read/29869500/towards-an-increased-functionality-in-oyster-pleurotus-mushrooms-produced-on-grape-marc-or-olive-mill-wastes-serving-as-sources-of-bioactive-compounds
#18
Georgios Koutrotsios, Nick Kalogeropoulos, Andriana C Kaliora, Georgios Zervakis
Pleurotus ostreatus, P. eryngii and P. nebrodensis were cultivated on non-conventional substrates containing grape marc (GMC) or olive mill by-products (OMB); wheat straw (WHS) served as control. GMC-based media demonstrated equal/better mushroom productivity than WHS for P. eryngii and P. nebrodensis, while the cultivation performance of P. eryngii was improved in OMB-based media. Both GMC and OMB substrates led to large increase of fruit-bodies content in phenolic acids, resveratrol, triterpenic compounds and ergosterol; in particular, P...
June 5, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29864664/interrogating-pollution-sources-in-a-mangrove-food-web-using-multiple-stable-isotopes
#19
Iara da C Souza, Hiulana P Arrivabene, Carol-Ann Craig, Andrew J Midwood, Barry Thornton, Silvia T Matsumoto, Michael Elliott, Daniel A Wunderlin, Magdalena V Monferrán, Marisa N Fernandes
Anthropogenic activities including metal contamination create well-known problems in coastal mangrove ecosystems but understanding and linking specific pollution sources to distinct trophic levels within these environments is challenging. This study evaluated anthropogenic impacts on two contrasting mangrove food webs, by using stable isotopes (δ13 C, δ15 N, 87 Sr/86 Sr, 206 Pb/207 Pb and 208 Pb/207 Pb) measured in sediments, mangrove trees (Rhizophora mangle, Laguncularia racemosa, Avicennia schaueriana), plankton, shrimps (Macrobranchium sp...
June 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29864587/the-first-morphologic-and-functional-characterization-of-hemocytes-in-hong-kong-oyster-crassostrea-hongkongensis
#20
Jun Li, Yuehuan Zhang, Fan Mao, Yue Lin, Shu Xiao, Zhiming Xiang, Haitao Ma, Yang Zhang, Ziniu Yu
Hemocytes are the first line of defence of the innate immune system of molluscs. For the first time hemocytes of Crassostrea hongkongensis were morphologically and functionally characterized, identifying circulating cell types and studying their involvement in immune responses. In the present study, two main populations, hyalinocytes and granulocytes, were characterized based on the presence or absence of cytoplasmic granules, using light and electron microscopy (TEM), and flow cytometry analyses. Granulocytes are 7-13 μm in diameter and present evident cytoplasmic granules, and hyalinocytes, 6-15 μm in diameter, with a few or no granules...
June 1, 2018: Fish & Shellfish Immunology
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