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https://www.readbyqxmd.com/read/28328985/construction-and-evaluation-of-a-high-density-snp-array-for-the-pacific-oyster-crassostrea-gigas
#1
Haigang Qi, Kai Song, Chunyan Li, Wei Wang, Busu Li, Li Li, Guofan Zhang
Single nucleotide polymorphisms (SNPs) are widely used in genetics and genomics research. The Pacific oyster (Crassostrea gigas) is an economically and ecologically important marine bivalve, and it possesses one of the highest levels of genomic DNA variation among animal species. Pacific oyster SNPs have been extensively investigated; however, the mechanisms by which these SNPs may be used in a high-throughput, transferable, and economical manner remain to be elucidated. Here, we constructed an oyster 190K SNP array using Affymetrix Axiom genotyping technology...
2017: PloS One
https://www.readbyqxmd.com/read/28324231/occurrence-and-virulence-properties-of-vibrio-and-salinivibrio-isolates-from-tropical-lagoons-of-the-southern-caribbean-sea
#2
Milagro Fernández-Delgado, Paula Suárez, Sandra Giner, Virginia Sanz, Jessy Peña, Damarys Sánchez, M Alexandra García-Amado
The Vibrionaceae are Gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. The aim of this research was to investigate the occurrence and virulence properties of Vibrio and Salinivibrio isolated from lagoons at Cuare Wildlife Refuge and Margarita Island in the southern Caribbean Sea. Water, plankton and oyster samples were collected during October 2011 and March 2012 and examined by specific PCR and culture methods...
March 21, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28320509/response-of-the-pearl-oyster-pinctada-margaritifera-to-cadmium-and-chromium-identification-of-molecular-biomarkers
#3
Gueguen Yannick, Saulnier Denis, Santini Adrien, Magre Kevin, Garen Pierre, Bernagout Solène, Nohl Marine, Bouisset Patrick, Helme Herehia, Planes Serge, Le Moullac Gilles
This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10μgL(-1)) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues...
March 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28318292/from-play-to-proficiency-the-ontogeny-of-stone-tool-use-in-coastal-foraging-long-tailed-macaques-macaca-fascicularis-from-a-comparative-perception-action-perspective
#4
Amanda W Y Tan
Macaques crack shellfish in coastal environments with specialized stone-hammering techniques. I provide the first examination of skill development from 866 object-manipulation and 7,400 tool-use bouts, collected over 15 months, using longitudinal analyses of infants' object manipulation (N = 7) and cross-sectional comparisons of manipulative and tool-use behavior (N = 69). I adopt a Perception-action approach, examining how the emergence of actions on objects relate to the spatial-relational and percussive challenges of tool use...
March 20, 2017: Journal of Comparative Psychology
https://www.readbyqxmd.com/read/28316573/a-production-calendar-based-on-water-temperature-spat-size-and-husbandry-practices-reduce-oshv-1-%C3%AE-var-impact-on-cultured-pacific-oyster-crassostrea-gigas-in-the-ebro-delta-catalonia-mediterranean-coast-of-spain
#5
Noèlia Carrasco, Ignasi Gairin, Josu Pérez, Karl B Andree, Ana Roque, Margarita Fernández-Tejedor, Chris J Rodgers, Cristobal Aguilera, M Dolors Furones
Since 2006, the production of Pacific oyster Crassostrea gigas in the Ebro Delta area has dramatically declined from around 800 metric tons (MT) per year to 138 MT in 2011. This decline in production has had a significant socio-economic impact in a region where the shellfish sector is a traditional economic activity for many families. The identified agent responsible for this reduction in C. gigas production was Ostreid Herpesvirus microvar (OsHV-1 μvar), which has been associated with C. gigas spat mortalities in France, and in many other countries...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28316346/invasion-trajectory-of-pacific-oysters-in-the-northern-wadden-sea
#6
Karsten Reise, Christian Buschbaum, Heike Büttger, Johannes Rick, K Mathias Wegner
Invasion trajectories of introduced alien species usually begin with a long establishment phase of low abundance, often followed by exponential expansion and subsequent adjustment phases. We review the first 26 years of feral Pacific oysters Crassostrea gigas around the island of Sylt in the Wadden Sea (North Sea, NE Atlantic), and reveal causal conditions for the invasion phases. Sea-based oyster farming with repeated introductions made establishment of feral oysters almost inevitable. Beds of mussels Mytilus edulis on mud flats offered firm substrate for attachment and ideal growth conditions around low tide level...
2017: Marine Biology
https://www.readbyqxmd.com/read/28315825/effects-of-florida-red-tides-on-histone-variant-expression-and-dna-methylation-in-the-eastern-oyster-crassostrea-virginica
#7
Rodrigo Gonzalez-Romero, Victoria Suarez-Ulloa, Javier Rodriguez-Casariego, Daniel Garcia-Souto, Gabriel Diaz, Abraham Smith, Juan Jose Pasantes, Gary Rand, Jose M Eirin-Lopez
Massive algal proliferations known as Harmful Algal Blooms (HABs) represent one of the most important threats to coastal areas. Among them, the so-called Florida Red Tides (FRTs, caused by blooms of the dinoflagellate Karenia brevis and associated brevetoxins) are particularly detrimental in the southeastern U.S., causing high mortality rates and annual losses in excess of $40 million. The ability of marine organisms to cope with environmental stressors (including those produced during HABs) is influenced by genetic and epigenetic mechanisms, the latter resulting in phenotypic changes caused by heritable modifications in gene expression, without involving changes in the genetic (DNA) sequence...
March 7, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28304108/differential-effects-of-zn-exposure-on-male-and-female-oysters-crassostrea-angulata-as-revealed-by-label-free-quantitative-proteomics
#8
Lianzhong Luo, Qinghong Zhang, Xue Kong, Heqing Huang, Weiwei You, Caihuan Ke
Oysters accumulate Zn as an adaptation to Zn exposure; however, it is not known whether male and female oysters respond differently to Zn exposure. Here, proteomic and real-time polymerase chain reaction analyses were used to investigate differential responses of male and female oysters (Crassostrea angulata) to Zn exposure. After exposure to 50 µg.L(-1) or 500 µg.L(-1) Zn for 30 days, gonads of female oysters accumulated more Zn than those of males, and gonadal development was accelerated in females but was abnormal in males...
March 17, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28286314/a-norepinephrine-responsive-mirna-directly-promotes-cghsp90aa1-expression-in-oyster-haemocytes-during-desiccation
#9
Hao Chen, Lusheng Xin, Xiaorui Song, Lin Wang, Weilin Wang, Zhaoqun Liu, Huan Zhang, Lingling Wang, Zhi Zhou, Limei Qiu, Linsheng Song
Oyster Crassostrea gigas is one model mollusc inhabiting in the intertidal zone and is frequently stressed by desiccation. The adaptation mechanism of oyster to environmental stress involves multiple levels, and miRNA is one of the most important regulators in post-transcriptional level. In the present study, an oyster norepinephrine-responsive miRNA cgi-miR-365 was proved to contribute to the host adaptation against desiccation by directly promoting the expression of CgHSP90AA1. Briefly, a significant increase of cgi-miR-365 was observed from the first day after aerial exposure and the up-regulation was vigorously repressed when oysters were injected with adrenoceptors antagonists...
March 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28283443/transcriptomic-analysis-of-oyster-crassostrea-gigas-larvae-illustrates-the-response-patterns-regulated-by-catecholaminergic-system-upon-acute-heat-and-bacterial-stress
#10
Zhaoqun Liu, Lingling Wang, Zhi Zhou, Yu Liu, Miren Dong, Weilin Wang, Xiaorui Song, Mengqiang Wang, Qiang Gao, Linsheng Song
Bacterial infection and heat stress, as two major environmental threats of marine molluscs, could affect larval development and dramatically promote mortality of oysters. In the present study, next-generation sequencing, together with determinations of mRNA expression and measurements of enzyme activities, were employed to understand the response patterns of oyster larvae under acute heat and bacterial stress. After RNA-seq, a total of 9472 differentially expressed genes including 4895 significantly up-regulated ones and 4577 significantly down-regulated ones were obtained from 12 transcriptome libraries...
March 7, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28281649/%C3%AE-15-n-values-in-crassostrea-virginica-shells-provides-early-direct-evidence-for-nitrogen-loading-to-chesapeake-bay
#11
H D Black, C F T Andrus, W J Lambert, T C Rick, D P Gillikin
Crassostrea virginica is one of the most common estuarine bivalves in the United States' east coast and is frequently found in archaeological sites and sub-fossil deposits. Although there have been several sclerochronological studies on stable carbon and oxygen isotopes in the shells of this species, less is known about δ(15)N values within their shells, which could be a useful paleoenvironmental proxy to assess estuarine nitrogen dynamics. Modern C. virginica samples were collected in Chesapeake Bay for comparison with archaeological shells from nearby sites ranging in age from ~100 to 3,200 years old...
March 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28280925/a-soxc-gene-related-to-larval-shell-development-and-co-expression-analysis-of-different-shell-formation-genes-in-early-larvae-of-oyster
#12
Gang Liu, Pin Huan, Baozhong Liu
Among the potential larval shell formation genes in mollusks, most are expressed in cells surrounding the shell field during the early phase of shell formation. The only exception (cgi-tyr1) is expressed in the whole larval mantle and thus represents a novel type of expression pattern. This study reports another gene with such an expression pattern. The gene encoded a SoxC homolog of the Pacific oyster Crassostrea gigas and was named cgi-soxc. Whole-mount in situ hybridization revealed that the gene was highly expressed in the whole larval mantle of early larvae...
March 9, 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/28279505/characterization-of-vibrio-parahaemolyticus-isolated-from-oysters-in-korea-resistance-to-various-antibiotics-and-prevalence-of-virulence-genes
#13
Chang-Ho Kang, YuJin Shin, SeokCheol Jang, HongSik Yu, SuKyung Kim, Sera An, Kunbawui Park, Jae-Seong So
Vibrio parahaemolyticus, found frequently in oysters, is the most prevalent gastroenteritis-causing pathogen in Korea and in several other Asian countries. This study monitored changes in the environmental parameters and occurrence of V. parahaemolyticus in oyster aquaculture sites. Of the 44 presumed V. parahaemolyticus isolates obtained, when tested against 16 antibiotics, 90.9, 86.4, and 75.0% of the 44 isolates exhibited resistance to vancomycin, ampicillin, and streptomycin, respectively. PCR analysis for the presence of the toxR gene confirmed 31 of the 44 isolates as being positive V...
March 6, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28274472/green-oysters-occurring-in-an-industrial-harbor-in-central-taiwan
#14
Tien-Hsi Fang, Shi-Yan Dai
Green oysters were found within the Mailiao industrial harbor (MIH) located in Central Taiwan. The metal concentrations in seawater (dissolved and particulate phases), sediment and oyster samples collected within the MIH were analyzed to examine the green oysters. The dissolved and particulate metal concentrations ranged within 0.008-1.47μgl(-1) and 0.97-799.2mgkg(-1), respectively. The Cu concentration in seawater was dominated in the dissolved phase. In contrast, Cr, Pb and Zn in seawater were chiefly present in the particulate phase...
March 5, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28274444/incorporation-of-dietary-fibre-rich-oyster-mushroom-pleurotus-sajor-caju-powder-improves-postprandial-glycaemic-response-by-interfering-with-starch-granule-structure-and-starch-digestibility-of-biscuit
#15
Sze Han Ng, Sathyasurya Daniel Robert, Wan Amir Nizam Wan Ahmad, Wan Rosli Wan Ishak
The purpose of this study was to determine the effects of Pleurotus sajor-caju (PSC) powder addition at 0, 4, 8 and 12% levels on the nutritional values, pasting properties, thermal characteristics, microstructure, in vitro starch digestibility, in vivo glycaemic index (GI) and sensorial properties of biscuits. Elevated incorporation levels of PSC powder increased the dietary fibre (DF) content and reduced the pasting viscosities and starch gelatinisation enthalpy value of biscuits. The addition of DF-rich PSC powder also interfered with the integrity of the starch granules by reducing the sizes and inducing the uneven spherical shapes of the starch granules, which, in turn, resulted in reduced starch susceptibility to digestive enzymes...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28273538/differential-effects-of-bisphenol-a-toxicity-on-oyster-crassostrea-angulata-gonads-as-revealed-by-label-free-quantitative-proteomics
#16
Lianzhong Luo, Qinghong Zhang, Xue Kong, Heqing Huang, Caihuan Ke
Bisphenol A (BPA) is widely used in industry, and is released in large amounts into the environment. BPA is a teratogen and an estrogen receptor agonist and negatively affects reproduction, particularly in aquatic animals, which is of concern for the aquaculture industry. Although there is a large body of literature on the mechanisms that underlie BPA disruption and the effects of different toxicities on invertebrate reproduction, many of the mechanisms involved in invertebrate responses to BPA remain unknown...
February 28, 2017: Chemosphere
https://www.readbyqxmd.com/read/28265512/predatory-blue-crabs-induce-stronger-nonconsumptive-effects-in-eastern-oysters-crassostrea-virginica-than-scavenging-blue-crabs
#17
Avery E Scherer, Miranda M Garcia, Delbert L Smee
By influencing critical prey traits such as foraging or habitat selection, predators can affect entire ecosystems, but the nature of cues that trigger prey reactions to predators are not well understood. Predators may scavenge to supplement their energetic needs and scavenging frequency may vary among individuals within a species due to preferences and prey availability. Yet prey reactions to consumers that are primarily scavengers versus those that are active foragers have not been investigated, even though variation in prey reactions to scavengers or predators might influence cascading nonconsumptive effects in food webs...
2017: PeerJ
https://www.readbyqxmd.com/read/28262379/oyster-based-national-mapping-of-trace-metals-pollution-in-the-chinese-coastal-waters
#18
Guang-Yuan Lu, Cai-Huan Ke, Aijia Zhu, Wen-Xiong Wang
To investigate the distribution and variability of trace metal pollution in the Chinese coastal waters, over 1000 adult oyster individuals were collected from 31 sites along the entire coastline, spanning from temperate to tropical regions (Bohai Sea, Yellow Sea, East China Sea and South China Sea), between August and September 2015. Concentrations of macroelements [sodium (Na), potassium (K), calcium (Ca), magnesium (Mg) and phosphorus (P)] and trace elements [cadmium (Cd), copper (Cu), zinc (Zn), nickel (Ni), lead (Pb), chromium (Cr), silver (Ag), and titanium (Ti)] in these oysters were concurrently measured and analyzed...
March 2, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28259423/the-constitutively-active-estrogen-receptor-er-binds-and-activates-the-promoter-of-the-vitellogenin-vtg-gene-in-the-sydney-rock-oyster-saccostrea-glomerata
#19
Thi Kim Anh Tran, Geoff R MacFarlane, Richard Yuen Chong Kong, Wayne A O'Connor, Richard Man Kit Yu
Vitellogenin (Vtg) is a well-established biomarker of estrogenic exposure in aquatic animals. In vertebrates, Vtg gene transcription is controlled by the estrogen receptors (ERs). Although an ER ortholog is present in molluscs, its role as a transcriptional regulator remains elusive. Here, we tested the hypothesis that in the Sydney rock oyster, Saccostrea glomerata, the ER ortholog activates Vtg gene transcription through specific interaction with its promoter. Luciferase reporter assays indicated that sgER activated both a minimal promoter containing the consensus estrogen-responsive elements (EREs) and the sgVtg promoter in an estrogen-independent manner...
March 1, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28252223/chronic-effects-of-copper-in-oysters-crassostrea-hongkongensis-under-different-exposure-regimes-as-shown-by-nmr-based-metabolomics
#20
Chen Cao, Wen-Xiong Wang
Traditional metal toxicity tests on organisms have mainly focused on continuous exposure at a fixed concentration. However, organisms are more likely exposed to pollutants intermittently in estuarine environments that are significantly impacted by anthropogenic activity. The present study examined whether different copper (Cu) exposure regimes at an equivalent dose can induce different metabolomics effects on the oysters. An estuarine oyster Crassostrea hongkongensis was exposed to Cu continuously or intermittently at an equal dose (time × concentration) for 6 wk...
March 2, 2017: Environmental Toxicology and Chemistry
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