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https://www.readbyqxmd.com/read/28330290/successful-cloning-of-an-adult-breeding-boar-from-the-novel-chinese-guike-no-1-swine-specialized-strain
#1
Jun-Yu Nie, Xiang-Xing Zhu, Bing-Kun Xie, Su-Qun Nong, Qing-Yan Ma, Hui-Yan Xu, Xiao-Gan Yang, Yang-Qing Lu, Ke-Huan Lu, Yu-Ying Liao, Sheng-Sheng Lu
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed is a novel swine specialized strain incorporated with the pedigree background of Duroc and Chinese Luchuan pig breeds, thus inherits an excellent production performance. The present study was conducted to establish somatic cloning procedures of adult breeding boars from the Chinese Guike No...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330165/genetic-characterization-and-population-structure-of-indian-rice-cultivars-and-wild-genotypes-using-core-set-markers
#2
Malathi Surapaneni, Divya Balakrishnan, Sukumar Mesapogu, Addanki Krishnam Raju, Yadavalli Venkateswara Rao, Sarla Neelamraju
Genetic diversity among 23 rice genotypes including wild species and cultivars of indica, japonica, aus and aromatic type was investigated using 165 genomewide core set microsatellite (SSR) markers. This genotypic characterization was undertaken to know the genetic similarity among the parental lines to be used in developing a set of chromosome segment substitution lines. In all, 253 alleles were identified using 77 polymorphic SSRs, and polymorphism information content ranged from 0.31 to 0.97 with a mean of 0...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330120/assessment-of-genetic-purity-in-rice-oryza-sativa-l-hybrids-using-microsatellite-markers
#3
Anjana Bora, Partha Ray Choudhury, Veena Pande, Asit B Mandal
The objective of the present study is to detect genetic impurity in the seed lots of CMS lines, restorers and hybrids and to identify signature markers to differentiate parents and hybrids through DNA-based assays. Furthermore, attempts have been made to find out an alternative to Grow-Out-Test, which is very tedious, time consuming and used conventionally for seed genetic purity testing since beginning of quality seed multiplication chain. Fifty-one rice-specific sequence tagged microsatellite (STMS) primer pairs distributed throughout the rice genome were employed for fingerprinting of eight rice hybrids and their parental lines with a view to assess variation within parental lines and to test the genetic purity of the commercial seed lots...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330092/development-of-molecular-method-for-sex-identification-in-date-palm-phoenix-dactylifera-l-plantlets-using-novel-sex-linked-microsatellite-markers
#4
Maryam, Muhammad Jafar Jaskani, Faisal Saeed Awan, Saeed Ahmad, Iqrar A Khan
Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Date palm is a dioecious and slow flowering and is very difficult to identify the gender of the trees until it reaches the reproductive age (5-10 years). A total of 12 microsatellite primers were used with 30 date palm samples, 14 parents (8 male + 6 females) and 16 progeny (developed from parents breeding) which showed that microsatellites were highly polymorphic, having a great number of alleles. A total of 124 alleles were characterized in 12 SSR loci...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329141/frequent-spread-of-plasmodium-vivax-malaria-maintains-high-genetic-diversity-at-the-myanmar-china-border-without-distance-and-landscape-barriers
#5
Eugenia Lo, Nancy Lam, Elizabeth Hemming-Schroeder, Jennifer Nguyen, Guofa Zhou, Ming-Chieh Lee, Zhaoqing Yang, Liwang Cui, Guiyun Yan
Background.: In Myanmar, civil unrest and the establishment of internally displaced persons (IDP) settlements along the Myanmar-China border have impacted malaria transmission. Methods.: Microsatellite markers were used to examine source-sink dynamics for Plasmodium vivax between IDP and surrounding villages in the border region. Genotypic structure and diversity were compared across the three years following the establishment of IDP in order to infer demographic history...
March 3, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28329005/rethinking-the-influence-of-hydroelectric-development-on-gene-flow-in-a-long-lived-fish-the-lake-sturgeon-acipenser-fulvescens
#6
Craig A McDougall, Amy B Welsh, Thierry Gosselin, W Gary Anderson, Patrick A Nelson
Many hydroelectric dams have been in place for 50 - >100 years, which for most fish species means that enough generations have passed for fragmentation induced divergence to have accumulated. However, for long-lived species such as Lake Sturgeon, Acipenser fulvescens, it should be possible to discriminate between historical population structuring and contemporary gene flow and improve the broader understanding of anthropogenic influence. On the Winnipeg River, Manitoba, two hypotheses were tested: 1) Measureable quantities of former reservoir dwelling Lake Sturgeon now reside downstream of the Slave Falls Generating Station, and 2) genetically differentiated populations of Lake Sturgeon occur upstream and downstream, a result of historical structuring...
2017: PloS One
https://www.readbyqxmd.com/read/28327965/carbon-dating-cancer-defining-the-chronology-of-metastatic-progression-in-colorectal-cancer
#7
H Lote, I Spiteri, L Ermini, A Vatsiou, A Roy, A McDonald, N Maka, M Balsitis, N Bose, M Simbolo, A Mafficini, A Lampis, J C Hahne, F Trevisani, Z Eltahir, G Mentrasti, C Findlay, Eaj Kalkman, M Punta, B Werner, S Lise, A Aktipis, C Maley, M Greaves, C Braconi, J White, M Fassan, A Scarpa, A Sottoriva, N Valeri
Background: HASH(0x467af60) Patients often ask oncologists how long a cancer has been present before causing symptoms or spreading to other organs. The evolutionary trajectory of cancers can be defined using phylogenetic approaches but lack of chronological references makes dating the exact onset of tumours very challenging. Patients and Methods: HASH(0x4a499e8) Here we describe the case of a colorectal cancer patient presenting with synchronous lung metastasis and metachronous thyroid, chest wall and urinary tract metastases over the course of five years...
February 23, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28327908/predicted-vitamin-d-status-and-colon-cancer-recurrence-and-mortality-in-calgb-89803-alliance
#8
M A Fuchs, C Yuan, K Sato, D Niedzwiecki, X Ye, L B Saltz, R J Mayer, R B Mowat, R Whittom, A Hantel, A Benson, D Atienza, M Messino, H Kindler, A Venook, F Innocenti, R S Warren, M M Bertagnolli, S Ogino, E L Giovannucci, E Horvath, J A Meyerhardt, K Ng
Background: HASH(0x4556d68) Observational studies suggest that higher levels of 25-hydroxyvitamin D 3 (25(OH)D) are associated with a reduced risk of colorectal cancer and improved survival of colorectal cancer patients. However, the influence of vitamin D status on cancer recurrence and survival of patients with stage III colon cancer is unknown. Patients and methods: HASH(0x4916380) We prospectively examined the influence of post-diagnosis predicted plasma 25(OH)D on outcome among 1,016 patients with stage III colon cancer who were enrolled in a National Cancer Institute-sponsored adjuvant therapy trial (CALGB 89803)...
March 15, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28327578/partial-support-for-the-central-marginal-hypothesis-within-a-population-reduced-genetic-diversity-but-not-increased-differentiation-at-the-range-edge-of-an-island-endemic-bird
#9
K M Langin, T S Sillett, W C Funk, S A Morrison, C K Ghalambor
Large-scale population comparisons have contributed to our understanding of the evolution of geographic range limits and species boundaries, as well as the conservation value of populations at range margins. The central-marginal hypothesis (CMH) predicts a decline in genetic diversity and an increase in genetic differentiation toward the periphery of species' ranges due to spatial variation in genetic drift and gene flow. Empirical studies on a diverse array of taxa have demonstrated support for the CMH. However, nearly all such studies come from widely distributed species, and have not considered if the same processes can be scaled down to single populations...
March 22, 2017: Heredity
https://www.readbyqxmd.com/read/28326093/the-complete-chloroplast-genome-of-wild-rice-oryza-minuta-and-its-comparison-to-related-species
#10
Sajjad Asaf, Muhammad Waqas, Abdul L Khan, Muhammad A Khan, Sang-Mo Kang, Qari M Imran, Raheem Shahzad, Saqib Bilal, Byung-Wook Yun, In-Jung Lee
Oryza minuta, a tetraploid wild relative of cultivated rice (family Poaceae), possesses a BBCC genome and contains genes that confer resistance to bacterial blight (BB) and white-backed (WBPH) and brown (BPH) plant hoppers. Based on the importance of this wild species, this study aimed to understand the phylogenetic relationships of O. minuta with other Oryza species through an in-depth analysis of the composition and diversity of the chloroplast (cp) genome. The analysis revealed a cp genome size of 135,094 bp with a typical quadripartite structure and consisting of a pair of inverted repeats separated by small and large single copies, 139 representative genes, and 419 randomly distributed microsatellites...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28325880/balancing-selection-maintains-polymorphisms-at-neurogenetic-loci-in-field-experiments
#11
Eija Lonn, Esa Koskela, Tapio Mappes, Mikael Mokkonen, Angela M Sims, Phillip C Watts
Most variation in behavior has a genetic basis, but the processes determining the level of diversity at behavioral loci are largely unknown for natural populations. Expression of arginine vasopressin receptor 1a (Avpr1a) and oxytocin receptor (Oxtr) in specific regions of the brain regulates diverse social and reproductive behaviors in mammals, including humans. That these genes have important fitness consequences and that natural populations contain extensive diversity at these loci implies the action of balancing selection...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28325827/sessile-serrated-polyps-and-colon-cancer-prevention
#12
Shahrooz Rashtak, Rafaela Rego, Seth R Sweetser, Frank A Sinicrope
Evidence suggests that up to one fifth of colorectal carcinomas (CRC) develop from serrated polyps, named for their pattern of colonic crypts, and include the sessile serrated adenoma/polyp (SSA/P) that has malignant potential. SSA/Ps are typically located in the proximal colon and have molecular features of hypermethylation of CpG islands in gene promoters and activating point mutations (V600E) in the BRAF oncogene. Both of these features are seen in sporadic CRCs with microsatellite instability (MSI) which is potentially consistent with an origin of these cancers from precursor SSA/Ps...
March 21, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28323864/population-genetic-structure-of-eelgrass-zostera-marina-on-the-korean-coast-current-status-and-conservation-implications-for-future-management
#13
Jae Hwan Kim, Ji Hyoun Kang, Ji Eun Jang, Sun Kyeong Choi, Min Ji Kim, Sang Rul Park, Hyuk Je Lee
Seagrasses provide numerous ecosystem services for coastal and estuarine environments, such as nursery functions, erosion protection, pollution filtration, and carbon sequestration. Zostera marina (common name "eelgrass") is one of the seagrass bed-forming species distributed widely in the northern hemisphere, including the Korean Peninsula. Recently, however, there has been a drastic decline in the population size of Z. marina worldwide, including Korea. We examined the current population genetic status of this species on the southern coast of Korea by estimating the levels of genetic diversity and genetic structure of 10 geographic populations using eight nuclear microsatellite markers...
2017: PloS One
https://www.readbyqxmd.com/read/28323777/msh6-past-and-present-and-muir-torre-syndrome-connecting-the-dots
#14
Meera Mahalingam
Sebaceous neoplasms such as adenoma, sebaceoma, and carcinoma, although sporadic in their occurrence, are clinically significant because of their association with Muir-Torre syndrome (MTS). MTS is a rare autosomal dominant genodermatosis characterized by the occurrence of sebaceous neoplasms and/or keratoacanthomas and visceral malignancies. MTS is usually the result of germline mutations in the DNA mismatch repair genes MSH2 and, albeit less commonly, MLH1. Although less know, MSH6 is yet another key player...
April 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28322705/three-divergent-subpopulations-of-the-malaria-parasite-plasmodium-knowlesi
#15
Paul C S Divis, Lee C Lin, Jeffrine J Rovie-Ryan, Khamisah A Kadir, Fread Anderios, Shamilah Hisam, Reuben S K Sharma, Balbir Singh, David J Conway
Multilocus microsatellite genotyping of Plasmodium knowlesi isolates previously indicated 2 divergent parasite subpopulations in humans on the island of Borneo, each associated with a different macaque reservoir host species. Geographic divergence was also apparent, and independent sequence data have indicated particularly deep divergence between parasites from mainland Southeast Asia and Borneo. To resolve the overall population structure, multilocus microsatellite genotyping was conducted on a new sample of 182 P...
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28321848/population-genetic-structure-and-comparative-diversity-of-smallmouth-bass-micropterus-dolomieu-congruent-patterns-from-two-genomes
#16
C A Stepien, S I Karsiotis, T J Sullivan, K E Klymus
Genetic diversity and divergence patterns of smallmouth bass Micropterus dolomieu spawning groups are analysed across its northern native range with mtDNA cytochrome b gene sequences and eight unlinked nuclear DNA microsatellite loci. Results reveal high levels of genetic variability and significant differences in allelic representation among populations (mtDNA: mean ± s.e., HD  = 0·50 ± 0·06, mean ± s.e., θST  = 0·41 ± 0·02 and microsatellites: mean ± s.e. HO  = 0·46 ± 0·03, mean ± s...
March 21, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28320414/epidermal-growth-factor-receptor-and-epididymis-invasion-as-prognostic-biomarkers-in-clinical-stage-i-testicular-germ-cell-tumours
#17
Miguel F Sanmamed, E Esteban, E Uriol, R Zarate, M Capelan, C Muriel, G Crespo, J P Berros, P Pardo-Coto, Q Perez, C Alvarez-Fernández, P Jiménez Fonseca, M Luque, A Astudillo
BACKGROUND: Inguinal orchiectomy is curative in 70-80% of clinical stage I testicular germ cell tumours (CS I TGCT). The identification of patients who are at low risk of relapse is critical to avoid unnecessary treatment. The aim of this study is to explore EGFR, hMLH-1/hMSH-2 and microsatellite instability (MSI) as potential prognostic factors of recurrence in CS I TGCT. METHODS: Fifty-six CS I TGCT patients who underwent inguinal orchiectomy were included in this study...
March 20, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28317542/overlap-of-latent-pathogens-in-the-botryosphaeriaceae-on-a-native-and-agricultural-host
#18
James W M Mehl, Bernard Slippers, Jolanda Roux, Michael J Wingfield
Some species of the Botryosphaeriaceae are capable of infecting a broad range of host plants. We studied the species diversity of Botryosphaeriaceae associated with marula (Sclerocarya birrea subsp. caffra, Anacardiaceae) trees in South Africa over two seasons, as well as species common to both S. birrea and adjacent mango (Mangifera indica, Anacardiaceae) trees in a subset of sites. Gene flow amongst populations of Botryosphaeriaceae shared on these tree species was tested using microsatellite markers. Twelve species were identified from S...
April 2017: Fungal Biology
https://www.readbyqxmd.com/read/28316112/molecular-ecology-studies-of-species-radiations-current-research-gaps-opportunities-and-challenges
#19
Marylaure de la Harpe, Margot Paris, Dirk N Karger, Jonathan Rolland, Michael Kessler, Nicolas Salamin, Christian Lexer
Understanding the drivers and limits of species radiations is a crucial goal of evolutionary genetics and molecular ecology, yet research on this topic has been hampered by the notorious difficulty of connecting micro- and macro-evolutionary approaches to studying the drivers of diversification. To chart the current research gaps, opportunities, and challenges of molecular ecology approaches to studying radiations, we examine the literature in the journal Molecular Ecology and re-visit recent high-profile examples of evolutionary genomic research on radiations...
March 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28314280/embryonal-origin-of-mtscc-of-kidney-may-explain-its-morphological-heterogeneity-diagnostic-impact-of-genetic-analysis
#20
Daniel Banyai, Fanni Vastag, Maria Yusenko, Peter Bugert, Gyula Kovacs
BACKGROUND/AIM: Previous genetic and morphologic characterisation of mucinous tubular and spindle cell carcinoma (MTSCC) have yielded controversial results. The aim of this study was to explain the phenotypic heterogeneity of MTSCC diagnosed by genetic means. MATERIALS AND METHODS: We analyzed 7 MTSCC by array CGH and microsatellite allelotyping and by histology for morphological variation. We worked-up two entire kidneys with MTSCC to find microscopic alterations...
March 2017: Anticancer Research
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