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https://www.readbyqxmd.com/read/28813039/comparative-assessment-of-ssr-and-snp-markers-for-inferring-the-population-genetic-structure-of-the-common-fungus-armillaria-cepistipes
#1
T Tsykun, C Rellstab, C Dutech, G Sipos, S Prospero
During the last years, simple sequence repeats (SSRs, also known as microsatellites) and single-nucleotide polymorphisms (SNPs) have become the most popular molecular markers for describing neutral genetic variation in populations of a wide range of organisms. However, only a limited number of studies has focused on comparing the performance of these two types of markers for describing the underlying genetic structure of wild populations. Moreover, none of these studies targeted fungi, the group of organisms with one of the most complex reproductive strategies...
August 16, 2017: Heredity
https://www.readbyqxmd.com/read/28811671/microsatellite-evidence-of-dispersal-mechanism-of-red-swamp-crayfish-procambarus-clarkii-in-the-pearl-river-basin-and-implications-for-its-management
#2
Jinlong Huang, Shaoqing Tang, Fengjin Cai, Yanfang Lin, Zhengjun Wu
Discerning the dispersal patterns of invasive species is critically important for the design of effective management strategies and the development of appropriate theoretical models predicting the spatial expansion of introduced populations. Post-introduction dispersal may occur naturally or via human transport, but for many organisms, assessing the relative contribution of each of these factors is difficult using traditional methods. Here, we explored the genetic patterns associated with the spread of red swamp crayfish (Procambarus clarkii) among 21 populations in the Pearl River basin and 2 peripheral populations in the Yangtze River basin...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811251/deletion-in-hsp110-t17-correlation-with-wild-type-hsp110-expression-and-prognostic-significance-in-microsatellite-unstable-advanced-gastric-cancers
#3
Kyung-Ju Kim, Tae Hun Lee, Jung Ho Kim, Nam-Yun Cho, Woo Ho Kim, Gyeong Hoon Kang
Deletion of the HSP110 T17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T17 repeat and expression of HSP110wt, using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs...
August 12, 2017: Human Pathology
https://www.readbyqxmd.com/read/28810861/the-implementation-of-long-lasting-insecticidal-bed-nets-has-differential-effects-on-the-genetic-structure-of-the-african-malaria-vectors-in-the-anopheles-gambiae-complex-in-dielmo-senegal
#4
Seynabou Sougoufara, Cheikh Sokhna, Nafissatou Diagne, Souleymane Doucouré, Pape MBacké Sembène, Myriam Harry
BACKGROUND: Mosquitoes belonging to the Anopheles gambiae complex are the main vectors of malaria in sub-Saharan Africa. Among these, An. gambiae, Anopheles coluzzii and Anopheles arabiensis are the most efficient vectors and are largely distributed in sympatric locations. However, these species present ecological and behavioural differences that impact their vectorial capacity and complicate vector-control efforts, mainly based on long-lasting insecticidal bed nets (LLINs) and indoor residual spraying (IRS)...
August 15, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28809943/genetic-evidence-for-subspecies-differentiation-of-the-himalayan-marmot-marmota-himalayana-in-the-qinghai-tibet-plateau
#5
Jingyan Yan, Hongjian Chen, Gonghua Lin, Qian Li, Jiarui Chen, Wen Qin, Jianping Su, Tongzuo Zhang
The primary host of plague in the Qinghai-Tibet Plateau (QTP), China, is Marmota himalayana, which plays an essential role in the maintenance, transmission, and prevalence of plague. To achieve a more clear insight into the differentiation of M. himalayana, complete cytochrome b (cyt b) gene and 11 microsatellite loci were analyzed for a total of 423 individuals from 43 localities in the northeast of the QTP. Phylogenetic analyses with maximum likelihood and Bayesian inference methods showed that all derived haplotypes diverged into two primary well-supported monophyletic lineages, I and II, which corresponded to the referential sequences of two recognized subspecies, M...
2017: PloS One
https://www.readbyqxmd.com/read/28808540/who-s-my-daddy-considerations-for-the-influence-of-sexual-selection-on-multiple-paternity-in-elasmobranch-mating-systems
#6
Kady Lyons, Chris L Chabot, Christopher G Mull, Corinne N Paterson Holder, Christopher G Lowe
Polyandry resulting in multiply-sired litters has been documented in the majority of elasmobranch species examined to date. Although commonly observed, reasons for this mating system remain relatively obscure, especially in batoids. The round stingray (Urobatis halleri) is an abundant, well-studied elasmobranch distributed throughout the northeastern Pacific that we used to explore hypotheses regarding multiple paternity in elasmobranchs. Twenty mid- to late-term pregnant females were sampled off the coast of southern California and their litters analyzed for the occurrence of multiple paternity using five nuclear microsatellite loci...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28807210/personalized-treatment-in-patients-with-colorectal-liver-metastases
#7
Dimitrios Moris, Timothy M Pawlik
BACKGROUND: Precision Medicine Initiative is a new research effort aiming to offer personalized treatment in many diseases, including cancer. The aim of the present article is to offer novel insights about the role of personalized treatment in patients with colorectal liver metastases (CRLM). METHODS: A review of the literature regarding personalized medicine and colorectal liver metastases was performed mainly in the MEDLINE/PubMed database. RESULTS: Surgical resection remains the only hope for cure of CRLM...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28806735/molecular-characterization-and-multi-locus-genotypes-of-enterocytozoon-bieneusi-from-captive-red-kangaroos-macropus-rfus-in-jiangsu-province-china
#8
Zhijun Zhong, Yinan Tian, Yuan Song, Lei Deng, Junxian Li, Zhihua Ren, Xiaoping Ma, Xiaobin Gu, Changliang He, Yi Geng, Guangneng Peng
Enterocytozoon bieneusi is the most common pathogen of microsporidian species infecting humans worldwide. Although E. bieneusi has been found in a variety of animal hosts, information on the presence of E. bieneusi in captive kangaroos in China is limited. The present study was aimed at determining the occurrence and genetic diversity of E. bieneusi in captive kangaroos. A total of 61 fecal specimens (38 from red kangaroos and 23 from grey kangaroos) were collected from Nanjing Hongshan Forest Zoo and Hongshan Kangaroo Breeding Research Base, Jiangsu province, China...
2017: PloS One
https://www.readbyqxmd.com/read/28805283/closing-the-gap-avian-lineage-splits-at-a-young-narrow-seaway-imply-a-protracted-history-of-mixed-population-response
#9
S A Trewick, S Pilkington, L D Shepherd, G C Gibb, M Morgan-Richards
The evolutionary significance of spatial habitat gaps has been well recognised since Alfred Russel Wallace compared the faunas of Bali and Lombok. Gaps between islands influence population structuring of some species, and flightless birds are expected to show strong partitioning even where habitat gaps are narrow. We examined the population structure of the most numerous living flightless land bird in New Zealand and the world, Weka (Gallirallus australis). We surveyed Weka and their feather lice in native and introduced populations using genetic data gathered from DNA sequences of mitochondrial genes and nuclear β-fibrinogen and five microsatellite loci...
August 14, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28803727/elimination-of-toxic-microsatellite-repeat-expansion-rna-by-rna-targeting-cas9
#10
Ranjan Batra, David A Nelles, Elaine Pirie, Steven M Blue, Ryan J Marina, Harrison Wang, Isaac A Chaim, James D Thomas, Nigel Zhang, Vu Nguyen, Stefan Aigner, Sebastian Markmiller, Guangbin Xia, Kevin D Corbett, Maurice S Swanson, Gene W Yeo
Microsatellite repeat expansions in DNA produce pathogenic RNA species that cause dominantly inherited diseases such as myotonic dystrophy type 1 and 2 (DM1/2), Huntington's disease, and C9orf72-linked amyotrophic lateral sclerosis (C9-ALS). Means to target these repetitive RNAs are required for diagnostic and therapeutic purposes. Here, we describe the development of a programmable CRISPR system capable of specifically visualizing and eliminating these toxic RNAs. We observe specific targeting and efficient elimination of microsatellite repeat expansion RNAs both when exogenously expressed and in patient cells...
August 8, 2017: Cell
https://www.readbyqxmd.com/read/28803425/frameshift-mutations-in-repeat-sequences-of-ank3-hacd4-tcp10l-tp53bp1-mfn1-lcmt2-rnmt-trmt6-mettl8-and-mettl16-genes-in-colon-cancers
#11
Su Yeon Yeon, Yun Sol Jo, Eun Ji Choi, Min Sung Kim, Nam Jin Yoo, Sug Hyung Lee
Diminished ANK3 contributes to cell survival by inhibiting detachment-induced apoptosis. TP53BP1 that interacts with p53 and MFN1 that encodes a mitochondrial membrane protein are considered to have tumor suppressor gene (TSG) functions. HACD4 involving fatty acid synthesis and TCPL10 with transcription regulation functions are considered TSGs. Many genes involved in DNA methylations such as LCMT2, RNMT, TRMT6, METTL8 and METTL16 are often perturbed in cancer. The aim of our study was to find whether these genes were mutated in colorectal cancer (CRC)...
August 12, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28799406/a-polymorphic-ggc-repeat-in-the-npas2-gene-and-its-association-with-melanoma
#12
Alessandra Franzoni, Elitza Markova-Car, Sanja Dević-Pavlić, Davor Jurišić, Cinzia Puppin, Catia Mio, Marila De Luca, Giulia Petruz, Giuseppe Damante, Sandra Kraljević Pavelić
Circadian clock regulation in mammals is controlled by feedback loops of a set of circadian genes. One of these circadian genes, NPAS2, encodes for a member of the bHLH-PAS class of transcription factors and is expressed in the forebrain and in some peripheral organs such as liver and skin. Other biological processes are also regulated by circadian genes. For example, NPAS2 is involved in cell proliferation, DNA damage repair and malignant transformation. Aberrant expression of clock genes has been previously observed in melanoma which led to our effort to sequence the NPAS2 promoter region in this cancer type...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28798757/establishing-the-bases-for-introducing-the-unexplored-portuguese-common-bean-germplasm-into-the-breeding-world
#13
Susana T Leitão, Marco Dinis, Maria M Veloso, Zlatko Šatović, Maria C Vaz Patto
Common bean (Phaseolus vulgaris L.) is among the most important grain legumes for human consumption worldwide. Portugal has a potentially promising common bean germplasm, resulting from more than five centuries of natural adaptation and farmers' selection. Nevertheless, limited characterization of this resource hampers its exploitation by breeding programs. To support a more efficient conservation of the national bean germplasm and promote its use in crop improvement, we performed, for the first time, a simultaneous molecular marker (21 microsatellites and a DNA marker for phaseolin-type diversity analysis) and seed and plant morphological characterization (14 traits) of 175 accessions from Portuguese mainland and islands traditional bean-growing regions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28797715/universal-point-of-care-testing-for-lynch-syndrome-in-patients-with-upper-tract-urothelial-carcinoma
#14
Michael J Metcalfe, Firas G Petros, Priya Rao, Maureen E Mork, Lianchun Xiao, Russell R Broaddus, Surena F Matin
BACKGROUND: Patients with Lynch syndrome (LS) are at risk for upper tract urothelial carcinoma (UTUC). We aim to identify the most reliable means of point-of-care (POC) screening for LS in UTUC patients. METHODS: From January 2013 through July 2016, 115 consecutive UTUC patients without a history of LS were universally screened during their follow-up. We evaluated patient/family history (Amsterdam criteria I [AMS1] and II [AMS2]), tumor immunohistochemistry (IHC) for mismatch repair proteins (MMRP), and microsatellite instability (MSI)...
August 7, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28797302/development-and-characterization-of-microsatellite-markers-for-diploid-populations-of-the-wind-pollinated-herb-mercurialis-annua
#15
Ana Paula Machado, John R Pannell, Jeanne Tonnabel
OBJECTIVE: Mercurialis annua is a wind-pollinated annual plant that has long been used as a model for the study of ploidy and sexual-systems evolution. However, no molecular markers are yet available for genetic studies of its diploid populations. Here, we develop and characterize a set of eight polymorphic microsatellite markers for diploid dioecious M. annua. RESULTS: Following an SSR-enrichment protocol, 13 microsatellite markers were proposed, eight of which yielded successful amplification and polymorphism...
August 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28796829/new-data-on-weddell-seal-leptonychotes-weddellii-colonies-a-genetic-analysis-of-a-top-predator-from-the-ross-sea-antarctica
#16
Ighor Antunes Zappes, Anna Fabiani, Valerio Sbordoni, Arnold Rakaj, Roberto Palozzi, Giuliana Allegrucci
In this paper, we studied the genetic variability in Weddell seal from colonies in Terra Nova Bay and Wood Bay, both sites located in the Ross Sea area, Antarctica. Two mitochondrial genes and one nuclear gene, with different mutation rates, were sequenced to investigate the haplotype diversity of the colonies and to test for a possible recent expansion. Fifteen microsatellites were used to analyze their genetic structure. Sequenced genes and microsatellites were also used to estimate the effective population size of the studied colonies and the Ross Sea seal population...
2017: PloS One
https://www.readbyqxmd.com/read/28796808/pd-l1-and-gastric-cancer-prognosis-a-systematic-review-and-meta-analysis
#17
Lihu Gu, Manman Chen, Dongyu Guo, Hepan Zhu, Wenchao Zhang, Junhai Pan, Xin Zhong, Xinlong Li, Haoran Qian, Xianfa Wang
The expression of Programmed cell Death Ligand 1 (PD-L1) is observed in many malignant tumors and is associated with poor prognosis including Gastric Cancer (GC). The relationship between PD-L1 expression and prognosis, however, is controversial in GC. This paper purports to use a meta-analysis to investigate the relationship between PD-L1 expression and prognosis in GC. For this study, the following databases were searched for articles published from June 2003 until February 2017: PubMed, EBSCO, Web of Science and Cochrane Library...
2017: PloS One
https://www.readbyqxmd.com/read/28796789/multiple-introductions-of-the-dengue-vector-aedes-aegypti-into-california
#18
Evlyn Pless, Andrea Gloria-Soria, Benjamin R Evans, Vicki Kramer, Bethany G Bolling, Walter J Tabachnick, Jeffrey R Powell
The yellow fever mosquito Aedes aegypti inhabits much of the tropical and subtropical world and is a primary vector of dengue, Zika, and chikungunya viruses. Breeding populations of A. aegypti were first reported in California (CA) in 2013. Initial genetic analyses using 12 microsatellites on collections from Northern CA in 2013 indicated the South Central US region as the likely source of the introduction. We expanded genetic analyses of CA A. aegypti by: (a) examining additional Northern CA samples and including samples from Southern CA, (b) including more southern US populations for comparison, and (c) genotyping a subset of samples at 15,698 SNPs...
August 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28795290/development-and-characterization-of-24-chloroplast-microsatellite-markers-for-two-species-of-eranthis-ranunculaceae
#19
Ami Oh, Byoung-Un Oh
Chloroplast microsatellites for two Korean endemic species, Eranthis byunsanensis and E. pungdoensis (Ranunculaceae), were isolated to address the questions of their distributional patterns and evolutionary relationships, using next-generation sequencing. Twenty-four polymorphic chloroplast microsatellite markers for these two species were developed, and then characterized in 65 individuals (55 individuals of E. byunsanensis and 10 individuals of E. pungdoensis). The number of alleles per locus ranged from 2 to 9; the average number of alleles across all the loci scored 4...
August 9, 2017: Molecular Biology Reports
https://www.readbyqxmd.com/read/28794806/recent-developments-in-the-treatment-of-metastatic-colorectal-cancer
#20
REVIEW
Jonathan M Loree, Scott Kopetz
Over the past decade there have been significant advances in the molecular characterization of colorectal cancer (CRC) that are driving treatment decisions. Expanded RAS testing beyond KRAS exon 2 was established as crucial for identifying patients who will respond to anti-epidermal growth factor receptor (EGFR) therapies and low-frequency mutations in RAS/tumor heterogeneity are gaining recognition as potential mechanisms of resistance. Despite this progress, the fact that we do not understand why left-sided but not right-sided tumors have improved outcomes following anti-EGFR therapy highlights our superficial understanding of this disease...
August 2017: Therapeutic Advances in Medical Oncology
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