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MiR200b

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https://www.readbyqxmd.com/read/27910778/mirsnps-of-mir1274-and-mir3202-genes-that-target-mecp2-and-dnmt3b-are-associated-with-lung-cancer-risk-a-study-conducted-on-massarray-genotyping
#1
Cansu Ozbayer, Irfan Degirmenci, Derya Ustuner, Guntulu Ak, Faruk Saydam, Ertugrul Colak, Hasan Veysi Gunes, Muzaffer Metintas
Genetic variants of miRNAs that target DNMTs and MBDs involved in DNA methylation were scanned with current databases, and 35 miRSNPs in 22 miRNA genes were identified. The aim of the study was to determine the association between these variants of miRNA genes and lung cancer (LC). DNA samples were isolated from blood samples and genotyped using a Sequenom MassARRAY System. An association between the rs188912830 gene variant of miR3202 that targets the MeCP2 protein and LC was indicated in both subtypes. The presence of the C-allele in patients with LC and its subtypes was significantly lower, and the absence of the C-allele was determined to increase the risk of LC by 7,429-times compared to the presence (p=0,010)...
2016: Journal of Environmental Pathology, Toxicology and Oncology
https://www.readbyqxmd.com/read/27685844/circulating-plasma-levels-of-microrna-21-and-microrna-221-are-potential-diagnostic-markers-for-primary-intrahepatic-cholangiocarcinoma
#2
Camilo Correa-Gallego, Danilo Maddalo, Alexandre Doussot, Nancy Kemeny, T Peter Kingham, Peter J Allen, Michael I D'Angelica, Ronald P DeMatteo, Doron Betel, David Klimstra, William R Jarnagin, Andrea Ventura
BACKGROUND: MicroRNAs (miRNAs) are potential biomarkers in various malignancies. We aim to characterize miRNA expression in intrahepatic cholangiocarcinoma (ICC) and identify circulating plasma miRNAs with potential diagnostic and prognostic utility. METHODS: Using deep-sequencing techniques, miRNA expression between tumor samples and non-neoplastic liver parenchyma were compared. Overexpressed miRNAs were measured in plasma from an independent cohort of patients with cholangiocarcinoma using RT-qPCR and compared with that healthy volunteers...
2016: PloS One
https://www.readbyqxmd.com/read/27666412/colorectal-cancer-cell-derived-microrna200-modulates-the-resistance-of-adjacent-blood-endothelial-barriers-in%C3%A2-vitro
#3
Silvio Holzner, Daniel Senfter, Serena Stadler, Anna Staribacher, Chi Huu Nguyen, Anna Gaggl, Silvana Geleff, Nicole Huttary, Sigurd Krieger, Walter Jäger, Helmut Dolznig, Robert M Mader, Georg Krupitza
Since cancer cells, when grown as spheroids, display drug sensitivity and radiation resistance patterns such as seen in vivo we recently established a three‑dimensional (3D) in vitro model recapitulating colorectal cancer (CRC)-triggered lymphatic endothelial cell (LEC)‑barrier breaching to study mechanisms of intra‑/extravasation. CRC metastasizes not only through lymphatics but also through blood vessels and here we extend the 3D model to the interaction of blood endothelial cells (BECs) with naïve and 5‑fluorouracil (5‑FU)‑resistant CRC CCL227 cells...
November 2016: Oncology Reports
https://www.readbyqxmd.com/read/27389411/microrna-153-targeting-of-kcnq4-contributes-to-vascular-dysfunction-in-hypertension
#4
Georgina Carr, Vincenzo Barrese, Jennifer B Stott, Oleksandr V Povstyan, Thomas A Jepps, Hericka B Figueiredo, Dongling Zheng, Yalda Jamshidi, Iain A Greenwood
AIMS: Kv7.4, a voltage-dependent potassium channel expressed throughout the vasculature, controls arterial contraction and is compromised in hypertension by an unknown mechanism. MicroRNAs (miRs) are post-transcriptional regulators of protein production and are altered in disease states such as hypertension. We investigated whether miRs regulate Kv7.4 expression. METHODS AND RESULTS: In renal and mesenteric arteries (MAs) of the spontaneously hypertensive rat (SHR), Kv7...
July 7, 2016: Cardiovascular Research
https://www.readbyqxmd.com/read/27184921/dna-methylation-signatures-of-early-childhood-malnutrition-associated-with-impairments-in-attention-and-cognition
#5
Cyril J Peter, Laura K Fischer, Marija Kundakovic, Paras Garg, Mira Jakovcevski, Aslihan Dincer, Ana C Amaral, Edward I Ginns, Marzena Galdzicka, Cyralene P Bryce, Chana Ratner, Deborah P Waber, David Mokler, Gayle Medford, Frances A Champagne, Douglas L Rosene, Jill A McGaughy, Andrew J Sharp, Janina R Galler, Schahram Akbarian
BACKGROUND: Early childhood malnutrition affects 113 million children worldwide, impacting health and increasing vulnerability for cognitive and behavioral disorders later in life. Molecular signatures after childhood malnutrition, including the potential for intergenerational transmission, remain unexplored. METHODS: We surveyed blood DNA methylomes (~483,000 individual CpG sites) in 168 subjects across two generations, including 50 generation 1 individuals hospitalized during the first year of life for moderate to severe protein-energy malnutrition, then followed up to 48 years in the Barbados Nutrition Study...
November 15, 2016: Biological Psychiatry
https://www.readbyqxmd.com/read/26201309/study-of-micrornas-mirnas-that-are-predicted-to-target-the-autoantigens-ro-ssa-and-la-ssb-in-primary-sj%C3%A3-gren-s-syndrome
#6
V C Gourzi, E K Kapsogeorgou, N C Kyriakidis, A G Tzioufas
The elevated tissue expression of Ro/SSA and La/SSB autoantigens appears to be crucial for the generation and perpetuation of autoimmune humoral responses against these autoantigens in Sjögren's syndrome (SS). The mechanisms that govern their expression are not known. miRNAs, the post-transcriptional regulators of gene expression, might be implicated. We have identified previously the miRNAs let7b, miR16, miR181a, miR200b-3p, miR200b-5p, miR223 and miR483-5p that are predicted to target Ro/SSA [Ro52/tripartite motif-containing protein 21 (TRIM21), Ro60/TROVE domain family, member 2 (TROVE2)] and La/SSB mRNAs...
October 2015: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/25226077/methylsorb-a-simple-method-for-quantifying-dna-methylation-using-dna-gold-affinity-interactions
#7
Abu Ali Ibn Sina, Laura G Carrascosa, Ramkumar Palanisamy, Sakandar Rauf, Muhammad J A Shiddiky, Matt Trau
The analysis of DNA methylation is becoming increasingly important both in the clinic and also as a research tool to unravel key epigenetic molecular mechanisms in biology. Current methodologies for the quantification of regional DNA methylation (i.e., the average methylation over a region of DNA in the genome) are largely affected by comprehensive DNA sequencing methodologies which tend to be expensive, tedious, and time-consuming for many applications. Herein, we report an alternative DNA methylation detection method referred to as "Methylsorb", which is based on the inherent affinity of DNA bases to the gold surface (i...
October 21, 2014: Analytical Chemistry
https://www.readbyqxmd.com/read/25119590/molecular-regulation-of-ovarian-cancer-cell-invasion
#8
Ningxia Sun, Qing Zhang, Chen Xu, Qian Zhao, Yan Ma, Xinmei Lu, Liang Wang, Wen Li
The molecular mechanism underlying ovarian cancer invasiveness and metastasis remains unclear. Since significant downregulation in microRNA 200 (miRNA200) family (miR200a, miR200b, and miR200c) has been reported in the invasive ovarian cancer cells, here, we used two human ovarian cancer cell lines, OVCAR3 and SKOV3, to study the molecular basis of miR200, matrix metalloproteinase 3 (MMP3) activation, and cancer invasiveness. We found that overexpression of either miR200 family member in OVCAR3 or SKOV3 cells significantly inhibited production and secretion of MMP3 and cancer invasiveness...
November 2014: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/24812058/androgen-receptor-as-a-regulator-of-zeb2-expression-and-its-implications-in-epithelial-to-mesenchymal-transition-in-prostate-cancer
#9
Sheeba Jacob, S Nayak, Gwendolyn Fernandes, R S Barai, S Menon, U K Chaudhari, S D Kholkute, Geetanjali Sachdeva
Zinc finger E-box-binding protein 2 (ZEB2) is known to help mediate the epithelial-to-mesenchymal transition, and thereby it facilitates cancer metastasis. This study was initiated to explore whether ZEB2 expression differs in prostate cancer (PCa, n=7) and benign prostatic hyperplasia (BPH, n=7) tissues. In PCa tissues, the levels of both immunoreactive ZEB2 and androgen receptor (AR) were found to be significantly higher (P<0.05) when compared with BPH tissues. Co-regulation of AR and ZEB2 prompted us to investigate the role of androgenic stimuli in ZEB2 expression...
June 2014: Endocrine-related Cancer
https://www.readbyqxmd.com/read/24667864/tgf-%C3%AE-1-stimulates-human-tenon-s-capsule-fibroblast-proliferation-by-mir-200b-and-its-targeting-of-p27-kip1-and-rnd3
#10
Jun Tong, Yuxuan Fu, Xinyu Xu, Shuxin Fan, Hong Sun, Ya Liang, Kai Xu, Zhilan Yuan, Yingbin Ge
PURPOSE: To evaluate the role of miR-200b expression in the proliferation of human Tenon's capsule fibroblasts (HTFs) induced by transforming growth factor-beta 1 (TGF-β1). METHODS: Human Tenon's capsule fibroblasts were treated with various doses of TGF-β1 for 24 hours. Cell proliferation was quantified by the cell counting kit-8 (cck-8) assay, cell cycle analysis, 5-ethynyl-2-deoxyuridine (EdU) assay, and analysis of cyclin E, cyclin D1, and proliferating cell nuclear antigen (PCNA) expression...
April 2014: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/24363810/aberrant-microrna-expression-in-endometrial-carcinoma-using-formalin-fixed-paraffin-embedded-ffpe-tissues
#11
COMPARATIVE STUDY
Taek Sang Lee, Hye Won Jeon, Yong Beom Kim, Young A Kim, Min A Kim, Soon Beom Kang
This study aimed to identify the candidate miRNAs in the carcinogenesis of endometrial carcinoma, and to explore whether FFPE material would be suitable for miRNA profiling. We identified the differences between miRNA expression profiles using human miRNA microarray in endometrioid endometrial adenocarcinomas (EECs) and normal endometria. Of those tested, miR-200a*, miR-200b*, miR-141, miR-182, and miR-205 were greatly enriched. The expressions of these five miRNAs were validated using quantitative real-time reverse transcription-PCR (qRT-PCR)...
2013: PloS One
https://www.readbyqxmd.com/read/24067373/activation-of-mir200-by-c-myb-depends-on-zeb1-expression-and-mir200-promoter-methylation
#12
Marco Pieraccioli, Francesca Imbastari, Alexey Antonov, Gerry Melino, Giuseppe Raschellà
Tumor progression to metastasis is a complex, sequential process that requires proliferation, resistance to apoptosis, motility and invasion to colonize at distant sites. The acquisition of these features implies a phenotypic plasticity by tumor cells that must adapt to different conditions by modulating several signaling pathways (1) during the journey to the final site of metastasis. Several transcription factors and microRNA play a role in tumor progression, but less is known about the control of their expression during this process...
July 15, 2013: Cell Cycle
https://www.readbyqxmd.com/read/23797579/activation-of-mir200-by-c-myb-depends-on-zeb1-expression-and-mir200-promoter-methylation
#13
Marco Pieraccioli, Francesca Imbastari, Alexey Antonov, Gerry Melino, Giuseppe Raschellà
Tumor progression to metastasis is a complex, sequential process that requires proliferation, resistance to apoptosis, motility and invasion to colonize at distant sites. The acquisition of these features implies a phenotypic plasticity by tumor cells that must adapt to different conditions by modulating several signaling pathways ( 1) during the journey to the final site of metastasis. Several transcription factors and microRNA play a role in tumor progression, but less is known about the control of their expression during this process...
June 24, 2013: Cell Cycle
https://www.readbyqxmd.com/read/22101269/tgf%C3%AE-induced-c-myb-affects-the-expression-of-emt-associated-genes-and-promotes-invasion-of-er-breast-cancer-cells
#14
Vincenzo Cesi, Arianna Casciati, Fabiola Sesti, Barbara Tanno, Bruno Calabretta, Giuseppe Raschellà
Advanced breast cancer cells acquire metastatic properties in response to TGFβ. We show here that the expression of c-Myb increases in TGFβ-treated ER (+) breast cancer cells by protein stabilization, transcription activation and release from miR200-dependent down-regulation. In particular, we mapped 2 sites for miR200b, miR200c and miR429 binding in the 3' UTR of the human c-myb gene. These microRNAs decreased the expression of c-Myb when transfected in MCF-7 cells. In addition, luciferase activity from a vector containing the 3' UTR of the c-myb gene was inhibited by miR200s through a binding-dependent mechanism...
December 1, 2011: Cell Cycle
https://www.readbyqxmd.com/read/22031875/change-in-micrornas-associated-with-neuronal-adaptive-responses-in-the-nucleus-accumbens-under-neuropathic-pain
#15
Satoshi Imai, Mai Saeki, Makoto Yanase, Hiroshi Horiuchi, Minako Abe, Michiko Narita, Naoko Kuzumaki, Tsutomu Suzuki, Minoru Narita
Neuropathic pain is the most difficult type of pain to control, and patients lose their motivation for the purposive pursuit with a decrease in their quality of life. Using a functional magnetic resonance imaging analysis, we demonstrated that blood oxygenation level-dependent signal intensity was increased in the ipsilateral nucleus accumbens (N.Acc.) following peripheral nerve injury. microRNAs are small, noncoding RNA molecules that direct the post-transcriptional suppression of gene expression, and play an important role in regulating synaptic plasticity...
October 26, 2011: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/20668090/cellular-micrornas-200b-and-429-regulate-the-epstein-barr-virus-switch-between-latency-and-lytic-replication
#16
Amy L Ellis-Connell, Tawin Iempridee, Iris Xu, Janet E Mertz
We previously showed that the cellular proteins ZEB1 and ZEB2/SIP1 both play key roles in regulating the latent-lytic switch of Epstein-Barr Virus (EBV) by repressing BZLF1 gene expression. We investigated here the effects of cellular microRNA (miRNA) 200 (miR200) family members on the EBV infection status of cells. We show that miR200b and miR429, but not miR200a, can induce EBV-positive cells into lytic replication by downregulating expression of ZEB1 and ZEB2, leading to production of infectious virus. The levels of miR200 family members in EBV-infected cells strongly negatively correlated with the levels of the ZEBs (e...
October 2010: Journal of Virology
https://www.readbyqxmd.com/read/20574809/mir152-mir200b-and-mir338-human-positional-and-functional-neuroblastoma-candidates-are-involved-in-neuroblast-differentiation-and-apoptosis
#17
Marco Ragusa, Alessandra Majorana, Barbara Banelli, Davide Barbagallo, Luisa Statello, Ida Casciano, Maria Rosa Guglielmino, Laura Rita Duro, Marina Scalia, Gaetano Magro, Cinzia Di Pietro, Massimo Romani, Michele Purrello
MicroRNAs (MIRs) perform critical regulatory functions within cell networks, both in physiology as well as in pathology. Through the positional gene candidate approach, we have identified three MIRs (MIR152, MIR200B, and MIR338) that are located in regions frequently altered in neuroblastoma (NB) and target mRNAs encoding proteins involved in cell proliferation, neuroblast differentiation, neuroblast migration, and apoptosis. Expression analysis in NB biopsies and NB cell lines showed that these MIRs are dysregulated...
October 2010: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
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