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Syed Aoun Ali, Deeba N Baig
Ezrin, Radixin and Moesin (ERM) are critical membranous component involved in cross-linking of actin filaments. Moesin (Msn) is recognized as a pivotal protein involved in regulation of cell signalling events associated with the maintenance of epithelial integrity, actin organization and polarity. Radixin (Rad) is known to cell-to-cell adherens junction as a barbed end-capping protein whereas ezrin (Ezr) is recognized at cell adhesion, motility, apoptosis and phagocytosis. The current study for the first time reports the transcriptional and RNA secondary structural variations among brain-specific ERM genes...
June 12, 2018: International Journal of Developmental Neuroscience
Roghayeh Sadeghi Erami, Karina Ovejero, Soraia Meghdadi, Marco Filice, Mehdi Amirnasr, Antonio Rodríguez-Diéguez, María Ulagares De La Orden, Santiago Gómez-Ruiz
Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole ( hpbtz ). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, 13 C CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection...
June 14, 2018: Nanomaterials
Khaled AbouAitah, Anna Swiderska-Sroda, Ahmed A Farghali, Jacek Wojnarowicz, Agata Stefanek, Stanislaw Gierlotka, Agnieszka Opalinska, Abdou K Allayeh, Tomasz Ciach, Witold Lojkowski
Naturally derived prodrugs have a wide range of pharmacological activities, including anticancer, antioxidant, and antiviral effects. However, significant barriers inhibit their use in medicine, e.g. their hydrophobicity. In this comprehensive study, we investigated simple and effective nanoformulations consisting of amine-functionalized and conjugated with folic acid (FA) mesoporous silica nanoparticles (MSNs). Two types of MSNs were studied: KCC- 1, with mean size 324 nm and mean pore diameter 3.4 nm, and MCM - 41, with mean size 197 and pore diameter 2 nm...
May 29, 2018: Oncotarget
Coline Pinese, Junquan Lin, Ulla Milbreta, Mingqiang Li, Yucai Wang, Kam W Leong, Sing Yian Chew
A low toxicity and efficient delivery system is needed to deliver small interfering RNAs (siRNA) in vitro and in vivo. The use of mesoporous silica nanoparticles (MSN) is becoming increasingly common due to its biocompatibility, tunable pore size and customizable properties. However, bolus delivery of siRNA/MSN complexes remains suboptimal, especially when a sustained and long-term administration is required. Here, we utilized electrospun scaffolds for sustained delivery of siRNA/MSN-PEI through surface adsorption and nanofiber encapsulation...
June 12, 2018: Acta Biomaterialia
Mithat Gunduz, Upendra A Argikar, Amanda L Cirello, Jennifer L Dumouchel
BACKGROUND: Acyl glucuronides of xenobiotics have been a subject of wide interest from the pharmaceutical industry with respect biochemical reactivity, hepatic disposition, and enterohepatic circulation. The reactivity and lack of stability of an acyl glucuronide for a clinical candidate could pose major developability concerns. To date, multiple in vitro assays have been published to assess the risk associated with acyl glucuronides. Despite this fact, the translation of these findings to predicting clinical safety remains poor...
June 10, 2018: Drug Metabolism Letters
Chengjian Wang, Shan Qiang, Wanjun Jin, Xuezheng Song, Ying Zhang, Linjuan Huang, Zhongfu Wang
Glycoproteins play pivotal roles in a series of biological processes and their glycosylation patterns need to be structurally and functionally characterized. However, the lack of versatile methods to release N-glycans as functionalized forms has been undermining glycomics studies. Here a novel method is developed for dissociation of N-linked glycans from glycoproteins for analysis by MS and online LC/MS. This new method employs aqueous ammonia solution containing NaBH3 CN as the reaction medium to release glycans from glycoproteins as 1-amino-alditol forms...
June 6, 2018: Journal of Proteomics
Ranran Cheng, Fanpeng Kong, Lili Tong, Xiaojun Liu, Kehua Xu, Bo Tang
Previous studies proposed that sodium selenite (Na2SeO3) was reduced to hydrogen selenide (H2Se) and that H2Se subsequently reacted with oxygen to generate superoxide anion (O2•-), resulting in tumor cell oxidative stress and apoptosis. However, under the hypoxic conditions of a solid tumor, the anticancer mechanism of sodium selenite remains unclear. To reveal the exact anticancer mechanism of selenite in the real tumor microenvironment, we developed a mitochondria-targeting fluorescent nanosensor, Mito-N-D-MSN, which was fabricated from mesoporous silica nanoparticles (MSNs) loaded with two small-molecule fluorescent probes and a triphenylphosphonium ion as a mitochondria-targeting moiety...
June 8, 2018: Analytical Chemistry
Joram D Mul, Marion Soto, Michael E Cahill, Rebecca E Ryan, Hirokazu Takahashi, Kawai So, Jia Zheng, Denise E Croote, Michael F Hirshman, Susanne E la Fleur, Eric J Nestler, Laurie J Goodyear
Elucidating mechanisms by which physical exercise promotes resilience, the brain's ability to cope with prolonged stress exposure while maintaining normal psychological functioning, is a major research challenge given the high prevalence of stress-related mental disorders, including major depressive disorder. Chronic voluntary wheel running (VWR), a rodent model that mimics aspects of human physical exercise, induces the transcription factor ΔFosB in the nucleus accumbens (NAc), a key reward-related brain area...
May 24, 2018: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
Nahum Zepeta-Flores, Mahara Valverde, Alejandro Lopez-Saavedra, Emilio Rojas
The importance of glutathione (GSH) in alternative cellular roles to the canonically proposed, were analyzed in a model unable to synthesize GSH. Gene expression analysis shows that the regulation of the actin cytoskeleton pathway is strongly impacted by the absence of GSH. To test this hypothesis, we evaluate the effect of GSH depletion via buthionine sulfoximine (5 and 12.5 mM) in human neuroblastoma MSN cells. In the present study, 70% of GSH reduction did not induce reactive oxygen species, lipoperoxidation, or cytotoxicity, which enabled us to evaluate the effect of glutathione in the absence of oxidative stress...
June 4, 2018: Genetics and Molecular Biology
Yifeng Cheng, Xuehua Wang, Xiaoyan Wei, Xueyi Xie, Sebastian Melo, Rajesh C Miranda, Jun Wang
BACKGROUND: Prenatal alcohol exposure (PAE) is a leading cause of hyperactivity in children. Excitation of dopamine D1 receptor-expressing medium spiny neurons (D1-MSNs) of the dorsomedial striatum (DMS), a brain region that controls voluntary behavior, is known to induce hyperactivity in mice. We therefore hypothesized that PAE-linked hyperactivity was due to persistently altered glutamatergic activity in DMS D1-MSNs. METHODS: Female Ai14 tdTomato-reporter mice were given access to alcohol in an intermittent-access, 2-bottle choice paradigm before pregnancy, and following mating with male D1-Cre mice, through the pregnancy period, and until postnatal day (P) 10...
June 5, 2018: Alcoholism, Clinical and Experimental Research
Shobhit Kumar Tiwari, Dilip Kumar Singh, Mayurbhai Kathadbhai Ladumor, Asit K Chakraborti, Saranjit Singh
Study of oxidative stability of pharmaceutical actives and formulations is important as oxidation pathway is the second most significant route for the decay of pharmaceuticals. Montelukast sodium, a leukotriene receptor antagonist, is prone to oxidation reactions owing to sensitive moieties in its structure. It is also known to be light sensitive. This study was aimed to understand the degradation behaviour of the drug in different oxidative media containing hydrogen peroxide, AIBN, Fe3+ , Fenton's reagent and O2 environment under normal laboratory light conditions...
May 26, 2018: Journal of Pharmaceutical and Biomedical Analysis
Onur Bender, Eulogio J Llorent-Martínez, Gokhan Zengin, Adriano Mollica, Ramazan Ceylan, Lucia Molina-García, Maria Luisa Fernández-de Córdova, Arzu Atalay
The genus Hypericum is one of the most popular genera in both traditional medicine and scientific platform. This study is designed to provide conceptual insights on the biological potential and chemical characterization of H. salsugineum, which is endemic to Turkey. The qualitative and quantitative phenolic content of the extracts was characterized by HPLC-ESI-MSn. Biological efficiency was investigated by enzyme inhibitory assays (cholinesterases, tyrosinase, amylase, and glucosidase) and anti-cancer efficacy tests (anti-proliferative activities with the iCELLigence technology, colony formation and wound healing scratch assays)...
2018: PloS One
Nikola Knezevic, Nebojsa Ilic, Veljko R Djokić, Rada Petrovic, Djordje Janackovic
Mesoporous silica nanoparticles (MSN) and periodic mesoporous organosilica nanoparticles containing bridging benzene (PMOBTB) and ethane (PMOBTE) moieties are synthesized, characterized and evaluated for application in skin protection from UVA/UVB sun irradiation. Furthermore, the influence of surface functionalization with chelating 3-(2-aminoethylamino)propylsilane and Zn2+ ions on the UV-blocking ability of MSN is evaluated, along with the photostability and capability of the synthesized nanomaterials to carry avobenzone, a known UV-absorbing agent...
June 4, 2018: ACS Applied Materials & Interfaces
Naima Rahmouni, Diana C G A Pinto, Noureddine Beghidja, Samir Benayache, Artur M S Silva
The phenolic profile of Scabiosa stellata L., a species used in Moroccan traditional medicine, is disclosed. To obtain that profile the species extract was analyzed by ultra-high-performance chromatography coupled to photodiode-array detection and electrospray ionization/ion trap mass spectrometry (UHPLC-DAD-ESI/MSn ). Twenty-five phenolic compounds were identified from which isoorientin and 4- O -caffeoylquinic acid can be highlighted because they are the major ones. The antioxidant activity was significantly controlled by the fraction type, with the n -butanol fraction showing the highest antioxidant activity (FRS50 = 64...
May 27, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Conner C Harper, Andrew G Elliott, Haw-Wei Lin, Evan R Williams
A general method for in situ measurements of the energy of individual ions trapped and weighed using charge detection mass spectrometry (CDMS) is described. Highly charged (> 300 e), individual polyethylene glycol (PEG) ions are trapped and oscillate within an electrostatic trap, producing a time domain signal. A segmented Fourier transform (FT) of this signal yields the temporal evolution of the fundamental and harmonic frequencies of ion motion throughout the 500-ms trap time. The ratio of the fundamental frequency and second harmonic (HAR) depends on the ion energy, which is an essential parameter for measuring ion mass in CDMS...
June 2, 2018: Journal of the American Society for Mass Spectrometry
Youxiu Lin, Qian Zhou, Yongyi Zeng, Dianping Tang
The authors describe a photoelectrochemical (PEC) immunoassay for determination of aflatoxin B1 (AFB1 ) in foodstuff. The competitive immunoreaction is carried out on a microplate coated with a capture antibody against AFB1 using AFB1 -bovine serum albumin (BSA)-liposome-coated mesoporous silica nanoparticles (MSN) loaded with L-cysteine as a support. The photocurrent is produced by a photoactive material consisting of cerium-doped Bi2 MoO6 . Initially, L-cysteine acting as the electron donor is gated in the pores by interaction between mesoporous silica and liposome...
June 2, 2018: Mikrochimica Acta
Xu Chen, Yanan Liu, Ange Lin, Na Huang, Liquan Long, Ye Gang, Jie Liu
Efflux pump system-mediated bacterial multidrug resistance is one of the main causes of antibiotic failure. Therefore, it is necessary to develop a novel nanocarrier that could effectively inhibit drug-resistant bacteria by increasing the intake and retention time of antibiotics. Herein, we constructed a pH-responsive nanocarrier (MSN@FA@CaP@FA) with double folic acid (FA) and calcium phosphate (CaP) covered on the surface of mesoporous silica (MSN) by electrostatic attraction and biomineralization, respectively...
May 31, 2018: Biomaterials Science
Xinjie Zhao, Zhongda Zeng, Aiming Chen, Xin Lu, Chunxia Zhao, Chunxiu Hu, Lina Zhou, Xinyu Liu, Xiaolin Wang, Xiaoli Hou, Yaorui Ye, Guowang Xu
Identification of the metabolites is an essential step in metabolomics study to interpret regulatory mechanism of pathological and physiological processes. However, it is still a big headache in LC-MSn-based studies because of the complexity of mass spectrometry, chemical diversity of metabolites, and deficiency of standards database. In this work, a comprehensive strategy is developed for accurate and batch metabolite identification in non-targeted metabolomics studies. First, a well defined procedure was applied to generate reliable and standard LC-MS2 data including tR, MS1 and MS2 information at a standard operational procedure (SOP)...
May 29, 2018: Analytical Chemistry
Zhaoyi Miao, Yue Hu, Xiaojing Zhang, Xiaotong Yang, Yingying Tang, An Kang, Dong Zhu
Currently, nanosphere-based ligand fishing cannot be accomplished with imaging processing, although this step is important for real-time identification. Herein, a ligand fishing technique combined with real-time imaging is presented for the identification of ligands for heat shock protein 90α (Hsp 90α) from a complex matrix, Alisma plantago-aquatica Linn. crude extract, using Hsp 90α-functionalized mesoporous silica nanoparticle (MSN)-InP/ZnS quantum dot (QD) nanocomposites as a support material. Twenty ligands for Hsp 90α were screened, and their structures were identified by mass spectrometry...
May 16, 2018: Journal of Chromatography. A
Bhoopendra Singh Kushwah, Jugal Gupta, Dilip Kumar Singh, Moolchand Kurmi, Archana Sahu, Saranjit Singh
Forced degradation studies on aliskiren were carried out according to ICH and WHO guidelines. Six degradation products were formed in total in the solution state. Their separation among themselves and from the drug was successfully achieved on a C-18 column utilizing acetonitrile and phosphate buffer (pH 3.0) in the mobile phase, which was run in a gradient mode. To characterize them, a complete mass fragmentation pathway of the drug was first established with the help of MS/TOF and MSn data. This was followed by LC-MS/TOF studies on the degradation products...
May 21, 2018: European Journal of Pharmaceutical Sciences
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