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https://www.readbyqxmd.com/read/29050247/rnai-mediated-knockdown-of-parp1-does-not-improve-the-development-of-female-cloned-mouse-embryos
#1
Guang-Yu Bai, Si-Hang Song, Rui-Zhen Sun, Zi-Hui Zhang, Jingyu Li, Zhen-Dong Wang, Zhong-Hua Liu, Lei Lei
Somatic cell nuclear transfer is an important technique for life science research, but its efficiency is still extremely low, and most genes that are important during early development, such as X chromosome-linked genes, are not appropriately expressed during this process. Poly (ADP-ribose) polymerase (PARP) is an enzyme that transfers ADP ribose clusters to target proteins. PARP family members such as PARP1 participate in cellular signalling pathways through poly (ADP-ribosylation) (PARylation), which ultimately promotes changes in chromatin structure, gene expression, and the localization and activity of proteins that mediate signalling responses...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29024917/thermo-responsive-mesoporous-silica-lipid-bilayer-hybrid-nanoparticles-for-doxorubicin-on-demand-delivery-and-reduced-premature-release
#2
Qing Zhang, Xuanxuan Chen, Huihui Shi, Gaoqiu Dong, Meiling Zhou, Tianji Wang, Hongliang Xin
Hybrid nanocarriers based on mesoporous silica nanoparticles (MSNs) and supported lipid bilayer (SLB) have been studied as drug delivery system. It still remains challenges to develop these nanocarriers (SLB-MSNs) with on-demand drug release profile for chemotherapy. Here, we reported the biocompatible SLB-MSNs with high drug loading, which could release doxorubicin (DOX) in response to hyperthermia and reduce premature release. After synthesis of MSNs via a sol-gel procedure, the thermo-responsive SLB was deposited on the MSNs by sonication to completely seal the mesopores...
October 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29019495/a-novel-visible-light-responsive-nanosystem-for-cancer-treatment
#3
M Martínez-Carmona, D Lozano, A Baeza, M Colilla, M Vallet-Regí
A novel singlet-oxygen sensitive drug delivery nanocarrier able to release its cargo after exposure to visible (Vis) light from a common lamp is presented. This nanodevice is based on mesoporous silica nanoparticles (MSN) decorated with porphyrin-caps grafted via reactive oxygen species (ROS)-cleavable linkages. In the presence of Vis light porphyrin-nanocaps produce singlet oxygen molecules that break the sensitive-linker, which triggers pore uncapping and therefore allows the release of the entrapped cargo (topotecan, TOP)...
October 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28978764/calcium-dynamics-control-k-atp-channel-mediated-bursting-in-substantia-nigra-dopamine-neurons-a-combined-experimental-and-modeling-study
#4
Christopher J Knowlton, Sylvie Kutterer, Jochen Roeper, Carmen C Canavier
Burst firing in medial substantia nigra dopamine (mSN DA) neurons has been selectively linked to novelty-induced exploration behavior in mice. Burst firing in mSN DA neurons, in contrast to lateral SN DA neurons, requires functional ATP-sensitive potassium channels (K-ATP) both in vitro and in vivo. However, the precise role of K-ATP channels in promoting burst firing is un-known. We show experimentally that L-type calcium channel activity in mSN DA neurons en-hances open probability of K-ATP channels. We then generated a mathematical model to study the role of Ca(2+) dynamics driving K-ATP channel function in mSN DA neurons during bursting...
October 4, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28977750/data-independent-mass-spectrometry-approach-for-screening-and-identification-of-dna-adducts
#5
Jingshu Guo, Peter W Villalta, Robert J Turesky
Long-term exposures to environmental toxicants and endogenous electrophiles are causative factors for human diseases including cancer. DNA adducts reflect the internal exposure to genotoxicants and can serve as biomarkers for risk assessment. Liquid chromatography-multistage mass spectrometry (LC-MS(n)) is the most common method for biomonitoring DNA adducts, generally targeting single exposures and measuring up to several adducts. However, the data often provide limited evidence for a role of a chemical in the etiology of cancer...
October 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28974022/effect-of-green-and-brown-propolis-extracts-on-the-expression-levels-of-micrornas-mrnas-and-proteins-related-to-oxidative-stress-and-inflammation
#6
Vincenzo Zaccaria, Valeria Curti, Arianna Di Lorenzo, Alessandra Baldi, Cristina Maccario, Sabrina Sommatis, Roberto Mocchi, Maria Daglia
A large body of evidence highlights that propolis exerts many biological functions that can be ascribed to its antioxidant and anti-inflammatory components, including different polyphenol classes. Nevertheless, the molecular mechanisms are yet unknown. The aim of this study is to investigate the mechanisms at the basis of propolis anti-inflammatory and antioxidant activities. The effects of two brown and green propolis extracts-chemically characterized by RP-HPLC-PDA-ESI-MSn-on the expression levels of miRNAs associated with inflammatory responses (miR-19a-3p and miR-203a-3p) and oxidative stress (miR-27a-3p and miR-17-3p), were determined in human keratinocyte HaCat cell lines, treated with non-cytotoxic concentrations...
October 1, 2017: Nutrients
https://www.readbyqxmd.com/read/28960952/polydopamine-like-coatings-as-payload-gatekeepers-for-mesoporous-silica-nanoparticles
#7
Miguel-Ángel Moreno-Villaécija, Josep Sedó-Vegara, Eduardo Guisasola, Alejandro Baeza, María Vallet Regí, Fabiana Nador, Daniel Ruiz-Molina
We report the use of bis-catecholic polymers as candidates for obtaining effective, tunable gatekeeping coatings for mesoporous silica nanoparticles (MSNs) intended for drug release applications. In monomers, catechol rings act as adhesive moieties and reactive sites for polymerization, together with middle linkers which may be chosen to tune the physicochemical properties of the resulting coating. Stable and low-toxicity coatings (pNDGA and pBHZ) were prepared from two bis-catechols of different polarity (NDGA and BHZ) on MSN carriers previously loaded with rhodamine B (RhB) as a model payload, by means of a previously reported synthetic methodology and without any previous surface modification...
September 29, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28958879/dual-targeting-mesoporous-silica-nanoparticles-for-inhibiting-tumour-cell-invasion-and-metastasis
#8
Wenqing Li, Zhaoming Guo, Kun Zheng, Kun Ma, Changhao Cui, Li Wang, Yue Yuan, Yu Tang
The invasion and metastasis of tumour cells are closely correlated with poor prognosis of cancer patients. In this study, a CD44 and N-cadherin dual targeting drug delivery system based on mesoporous silica nanoparticles (MSNs) has been successfully constructed for inhibiting tumour cell invasion and metastasis. Amino modified MSN (MSN/NH2) was first synthesized and then functionalized with hyaluronic acid (HA) and ADH-1, constructing the carrier ADH-1-HA-MSN. Doxorubicin hydrochloride (DOX) was selected as a model anticancer drug...
September 25, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28953354/simultaneous-and-sequential-ms-ms-scan-combinations-and-permutations-in-a-linear-quadrupole-ion-trap
#9
Dalton T Snyder, Lucas J Szalwinski, R Graham Cooks
Methods of performing precursor ion scans as well as neutral loss scans in a single linear quadrupole ion trap have recently been described. In this paper we report methodology for performing permutations of MS/MS scan modes, that is, ordered combinations of precursor, product, and neutral loss scans following a single ion injection event. Only particular permutations are allowed; the sequences demonstrated here are (1) multiple precursor ion scans, (2) precursor ion scans followed by a single neutral loss scan, (3) precursor ion scans followed by product ion scans, and (4) segmented neutral loss scans...
October 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28952259/-rapid-characterization-of-chemical-constituents-in-capillary-wormwood-extract-based-on-uhplc-ltq-orbitrap
#10
Wen-Zhu Ouyang, Zhan-Peng Shang, Wen-Jian Wang, Shou-Xu Wang, Jia-Xu Shen, Hao Qian, Zhi-Guo Ma, Jia-Yu Zhang
A rapid and sensitive UHPLC-HR-MSn method was developed for the identification of chemical constituents in capillary wormwood extract. ACQUITY UHPLC HSS T3 chromatography column (2.1 mm×100 mm, 1.7 μm) was used with 0.1% formic acid-acetonitrile solution as the mobile phase in gradient elution. The extract was detected by ESI-LTQ-Orbitrap equipped with an ESI ion source in a negative mode. Based on the accurate mass measurements, retention time, mass fragmentation patterns and literature reports, a total of 50 compounds including 21 flavonoids, 22 phenolic acids, 6 coumarins and 1 other compound were tentatively screened and characterized...
February 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28933118/-influence-of-different-drying-methods-on-steroidal-saponins-and-homoisoflavonoids-in-ophiopogon-japonicus
#11
Fei Wang, Zhan-Peng Shang, Zhi-Guo Ma, Si-Yi Liu, Zi-Jian Wang, Jia-Yu Zhang, Jian-Qiu Lu
To study the influence of three different drying methods (including 50 ℃-drying, 80 ℃-drying and -70 ℃-freeze-drying methods) on steroidal saponins and homoisoflavonoids in Ophiopogon japonicus,a HPLC-DAD-ELSD-MSn method was investigated to screen and identify the differential components. Through comparing the HPLC chromatograms with that of fresh O. japonicus, 50 ℃-drying medicine was similar with fresh medicine whereas the other two drying methods had great influence on the components of O. japonicus...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28924051/the-stress-regulatory-transcription-factors-msn2-and-msn4-regulate-fatty-acid-oxidation-in-budding-yeast
#12
Praveen Kumar Rajvanshi, Madhuri Arya, Ram Rajasekharan
The transcription factors multicopy suppressor of SNF1 mutation (Msn) Msn2 and Msn4 bind the stress-response element in gene promoters in the yeast Saccharomyces cerevisiae. However, the roles of Msn2/4 in primary metabolic pathways such as fatty acid β-oxidation are unclear. Here, in silico analysis revealed that the promoters of most genes involved in the biogenesis, function, and regulation of the peroxisome contain Msn2/4 binding sites. We also found that transcript levels of MSN2/MSN4 are increased in glucose-depletion conditions and that during growth in nonpreferred carbon sources, Msn2 is constantly localized to the nucleus in wildtype cells...
September 18, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28923978/mcam-contributes-to-the-establishment-of-cell-autonomous-polarity-in-myogenic-and-chondrogenic-differentiation
#13
Artal Moreno-Fortuny, Laricia Bragg, Giulio Cossu, Urmas Roostalu
Cell polarity has a fundamental role in shaping the morphology of cells and growing tissues. Polarity is commonly thought to be established in response to extracellular signals. Here we used a minimal in vitro assay that enabled us to monitor the determination of cell polarity in myogenic and chondrogenic differentiation in the absence of external signalling gradients. We demonstrate that the initiation of cell polarity is regulated by melanoma cell adhesion molecule (MCAM). We found highly polarized localization of MCAM, Moesin (MSN), Scribble (SCRIB) and Van-Gogh-like 2 (VANGL2) at the distal end of elongating myotubes...
September 18, 2017: Biology Open
https://www.readbyqxmd.com/read/28921966/tool-for-rapid-analysis-of-glycopeptide-by-permethylation-via-one-pot-site-mapping-and-glycan-analysis
#14
Asif Shajahan, Nitin T Supekar, Christian Heiss, Mayumi Ishihara, Parastoo Azadi
To overcome the challenges in the analysis of protein glycosylation, we have developed a comprehensive and universal tool through permethylation of glycopeptides and their tandem mass spectrometric analysis. This method has the potential to simplify glycoprotein analysis by integrating glycan sequencing and glycopeptide analysis in a single experiment. Moreover, glycans with unique glycosidic linkages, particularly from prokaryotes, which are resistant to enzymatic or chemical release, could also be detected and analyzed by this methodology...
October 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28921950/highly-efficient-and-rapid-neural-differentiation-of-mouse-embryonic-stem-cells-based-on-retinoic-acid-encapsulated-porous-nanoparticle
#15
Se-Jin Park, Seongchan Kim, Sung-Yon Kim, Noo Li Jeon, Joon Myong Song, Cheolhee Won, Dal-Hee Min
An improved cell conversion strategy for neural differentiation of mouse embryonic stem (mES) cells is developed by incorporating functionalized mesoporous silica nanoparticle (MSN) as an efficient delivery carrier of retinoic acid (RA), which is a pleiotropic factor required for initiation of neural differentiation. Traditional RA-mediated neural differentiation methods required either preactivation of the cells to the differentiating state by embryoid body (EB) formation or repetitive treatment of the differentiation factor...
October 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28917803/atp-mediated-rolling-circle-amplification-and-opening-dna-gate-for-drug-delivery-to-cell
#16
Yujie Wang, Xinxin Shang, Jia Liu, Yingshu Guo
Here, we have developed a facile fluorometric system for the detection of adenosine triphosphate (ATP) by a rolling circle amplification (RCA) based on proximity ligation mediated amplification, and simultaneously achieved the release of the anticancer drug doxorubicin (DOX) through the mesoporous silicon system. Once the ATP molecule is present, the linker DNA will be released from the graphene oxide (GO) surface and hybridized to the template DNA of the GO surface joining with ligation enzyme. RCA reaction is followed by the addition of the phi29 DNA polymerase...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28895809/screening-brief-intervention-and-referral-to-treatment-sbirt-curriculum-integration-and-sustainability-social-work-and-nursing-faculty-perspectives
#17
M Nicole Belfiore, Marcela D Blinka, Karlynn BrintzenhofeSzoc, Joseph Shields
BACKGROUND: The Substance Abuse and Mental Health Services Administration (SAMHSA) has funded grants to universities to provide training and conduct research on the dissemination of screening, brief intervention, and referral to treatment (SBIRT) to health care professionals. However, when it comes to integrating SBIRT content into an existing curriculum, difficulties can arise. When there is so much content already in the curriculum, adding more can be challenging. Additionally, some faculty believe that course curricula should be driven by the expertise and knowledge of faculty, not by opportunities afforded because of grant funding...
September 12, 2017: Substance Abuse
https://www.readbyqxmd.com/read/28894298/molecular-basis-of-dendritic-atrophy-and-activity-in-stress-susceptibility
#18
T C Francis, R Chandra, A Gaynor, P Konkalmatt, S R Metzbower, B Evans, M Engeln, T A Blanpied, M K Lobo
Molecular and cellular adaptations in nucleus accumbens (NAc) medium spiny neurons (MSNs) underlie stress-induced depression-like behavior, but the molecular substrates mediating cellular plasticity and activity in MSN subtypes in stress susceptibility are poorly understood. We find the transcription factor early growth response 3 (EGR3) is increased in D1 receptor containing MSNs of mice susceptible to social defeat stress. Genetic reduction of Egr3 levels in D1-MSNs prevented depression-like outcomes in stress susceptible mice by preventing D1-MSN dendritic atrophy, reduced frequency of excitatory input and altered in vivo activity...
September 12, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28888000/ph-and-redox-dual-stimulate-responsive-nanocarriers-based-on-hyaluronic-acid-coated-mesoporous-silica-for-targeted-drug-delivery
#19
Jian-Tao Lin, Ji-Kun Du, Yi-Qiu Yang, Li Li, Da-Wei Zhang, Cui-Ling Liang, Jie Wang, Jun Mei, Guan-Hai Wang
In this work, we developed a drug-conjugated nanocarrier with "zero premature release" property for actively targeted drug delivery. The pH and redox dual-responsive nanocarrier was fabricated based on hyaluronic acid (HA) modified the mesoporous silica nanoparticles (MSNs). Doxorubicin (DOX) was conjugated to MSNs via hydrazone bonds, which can be cleaved in tumor tissue (acidic conditions). To improve specific cellular uptake and stability of nanocarriers, HA was equipped with an outer shell on the nanoparticle surface via a disulfide crosslinker...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28877224/transformation-products-elucidation-of-forchlorfenuron-in-postharvest-kiwifruit-by-time-of-flight-mass-spectrometry
#20
Zhiwei Zhang, Zhenhong Gao, Yuan Wang, Yahong Yuan, Jing Dong, Tianli Yue
Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea, FCF) is a plant growth regulator, being extensively used for increasing kiwifruit size. The toxicological properties of its may persist in their transformation products (TPs) or even higher toxicity than FCF. TPs elucidation of FCF in postharvest kiwifruit (Actinidia chinensis, Chinese gooseberry) by the liquid chromatography ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) in positive mode was the objective of the present study...
2017: PloS One
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