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Nanofiber scaffold

Jessica H Brown, Prativa Das, Michael D DiVito, David Ivancic, Lay Poh Tan, Jason A Wertheim
A major challenge of maintaining primary hepatocytes in vitro is progressive loss of hepatocyte-specific functions, such as protein synthesis and cytochrome P450 (CYP450) catalytic activity. We developed a three-dimensional (3D) nanofibrous scaffold made from poly(L-lactide-co-glycolide) (PLGA) polymer using a newlyoptimizedwet electrospinning techniquethat resulted in a highly porous structure that accommodated inclusion of primary human hepatocytes. Extracellular matrix (ECM) proteins (type I collagen or fibronectin) at varying concentrations were chemically linked to electrospun PLGA using amine coupling to develop an in vitro culture system containing the minimal essential ECM components of the liver micro-environment that preserve hepatocyte function in vitro...
February 14, 2018: Acta Biomaterialia
S Uma Maheshwari, K Govindan, M Raja, A Raja, M B S Pravin, S Vasanth Kumar
β Tricalcium phosphate ceramic was used to reinforce nanofibers in composite mats produced via electrospinning of poly(vinyl alcohol) (PVA), polycaprolactone (PCL) and (PVA: PCL) bilayers. The role of TCP ceramic on morphology of nanocomposites, crystalline structure, functional groups and thermal behaviour of nanocomposites were characterized by SEM, EDAX, XRD, FTIR and DSC analysis. Ultrathin cross-sections of the obtained nanocomposites were morphologically investigated with SEM and all fabricated composites consisted of fibers with average fiber diameter (AFD) around 100 nm except PCL-TCP fibers having AFD in the range of 608 nm...
March 1, 2018: Journal of Nanoscience and Nanotechnology
Sukhwinder K Bhullar, Deepti Rana, Burçak Kaya Ozsel, Mehmet Orhan, Martin B G Jun, Harpal S Buttar, Serge Ostrovidov, Murugan Ramalingam
In this article, we report a simple, cost-effective and eco-friendly method of airbrushing for the fabrication of antibacterial composite nanofibers using Nylon-6 and silver chloride (AgCl). The Nylon-6 is a widely used polymer for various biomedical applications because of its excellent biocompatibility and mechanical properties. Similarly, silver has also been known for their antibacterial, antifungal, antiviral, and anti-inflammatory properties. In order to enhance the antibacterial functionality of the Nylon-6, composite nanofibers in combination with AgCl have been fabricated using airbrush method...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Zhiying Wang, He Shen, Saijie Song, Liming Zhang, Wei Chen, Jianwu Dai, Zhijun Zhang
Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in combination with biomaterial scaffolds, has raised increasing interests from researchers in tissue engineering. Nanofibers fabricated by electrospinning technique mimicking extracellular matrix (ECM) are widely used in tissue engineering applications...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Xiaoying Wang, Yijie Wang, Meng Jiang, Yanqun Shan, Xin Jin, Miao Gong, Xiaoning Wang
A functional electrospun nanofibers-based electrochemiluminescence (ECL) immunosensor for detection of the tumor suppressor protein p53 (TSP53) at trace level using core-shell nanocomposite as signal enhancers is fabricated. The gold nanoparticles (AuNPs) were decorated on the surface of the electrospun carbon-nanotubes (MWCNTs) doped chitosan (CTS) nanofibers (MWCNTs-CTS) by in-situ electrodeposition. The functional electrospun nanofibers (MWCNTs-CTS-AuNPs) was utilized as supporting scaffolds for TSP53 capture antibody (CAb) immobilization firstly...
February 9, 2018: Analytical Biochemistry
Mercedes Pérez-Recalde, Ignacio E Ruiz Arias, Élida B Hermida
BACKGROUND: Millions of people in the world suffer from chronic wounds of different etiologies such as diabetic foot and leg ulcers, without solutions nowadays. Molecules obtained from plants offer an alternative to aid wound healing. Strong evidence about essential oils (EO) anti-inflammatory and antimicrobial properties is thoroughly described in literature and their chemical compositions are well characterized. More recently, EO effects in experimental wounds have begun to be analyzed...
January 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Tong Wu, Jiajia Xue, Haoxuan Li, Chunlei Zhu, Xiumei Mo, Younan Xia
Scaffolds functionalized with circular gradients of active proteins are attractive for tissue regeneration due to their enhanced capability to accelerate cell migration and/or promote neurite extension in a radial fashion. Here we report a general method for generating circular gradients of active proteins on scaffolds comprised of radially aligned nanofibers. In a typical process, the scaffold, with its central portion raised using a copper wire to take a cone shape, was placed in a container (upright or up-side-down), followed by dropwise addition of a bovine serum albumin (BSA) solution into the container...
February 8, 2018: ACS Applied Materials & Interfaces
Wei Ji, Shogo Onomura, Toshio Sasaki, Ye Zhang
Extracellular matrix (ECM) is the natural fibrous scaffold that regulates cell behaviors in a hierarchical manner. By mimicking the dynamic and reciprocal interactions between ECM and cells, we developed matrix-mediated self-assembly (SA) of organic molecules attaining higher-order nanostructure. Two self-sorted coumarin-based gelators, one peptide molecule and one benzoate molecule that self-assemble into nanofibers and nanobelts in different time scales, respectively, are designed and synthesized. Upon the SA of peptide gelators forming fibrous scaffold, nanobelts transform into layer-by-layer nanosheet reaching 9-fold increase in height...
February 7, 2018: Angewandte Chemie
Martin Humenik, Gregor Lang, Thomas Scheibel
Natural silk fibers represent one of the most advanced blueprints for (bio)polymer scientists, displaying highly optimized mechanical properties due to their hierarchical structures. Biotechnological production of silk proteins and implementation of advanced processing methods enabled harnessing the potential of these biopolymer not just based on the mechanical properties. In addition to fibers, diverse morphologies can be produced, such as nonwoven meshes, films, hydrogels, foams, capsules and particles. Among them, nanoscale fibrils and fibers are particularly interesting concerning medical and technical applications due to their biocompatibility, environmental and mechanical robustness as well as high surface-to-volume ratio...
February 2, 2018: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Tomáš Suchý, Monika Šupová, Martin Bartoš, Radek Sedláček, Marco Piola, Monica Soncini, Gianfranco Beniamino Fiore, Pavla Sauerová, Marie Hubálek Kalbáčová
Collagen composite scaffolds have been used for a number of studies in tissue engineering. The hydration of such highly porous and hydrophilic structures may influence mechanical behaviour and porosity due to swelling. The differences in physical properties following hydration would represent a significant limiting factor for the seeding, growth and differentiation of cells in vitro and the overall applicability of such hydrophilic materials in vivo. Scaffolds based on collagen matrix, poly(DL-lactide) nanofibers, calcium phosphate particles and sodium hyaluronate with 8 different material compositions were characterised in the dry and hydrated states using X-ray microcomputed tomography, compression tests, hydraulic permeability measurement, degradation tests and infrared spectrometry...
February 1, 2018: Journal of Materials Science. Materials in Medicine
Xueshi Luo, Zhenzhao Guo, Ping He, Tian Chen, Lihua Li, Shan Ding, Hong Li
Collagen electrospun scaffolds properly reproduce the framework of the extracellular matrix (ECM) of tissues that are natural with the fibrous morphology of the protein by coupling large biomimetism of the biological material. However, traditional solvents employed for collagen electrospinning lead to poor mechanical attributes and bad hydro-stability. In this work, by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride with N-hydroxysulfosuccinimide (EDC-NHS), glutaraldehyde (GTA) and genipin (GP) respectively, electrospun collagen fibers cross-linked, effectively stabilized the fiber morphology over 2 months and improved the mechanical properties in both dry and wet state, especially EDC-NHS with large ultimate tensile stress and εb...
January 29, 2018: International Journal of Biological Macromolecules
Ruibo Zhong, Qian Tang, Shaopeng Wang, Hongbo Zhang, Feng Zhang, Mingshu Xiao, Tiantian Man, Xiangmeng Qu, Li Li, Weijia Zhang, Hao Pei
Conducting hydrogels provide great potential for creating designer shape-morphing architectures for biomedical applications owing to their unique solid-liquid interface and ease of processability. Here, a novel nanofibrous hydrogel with significant enzyme-like activity that can be used as "ink" to print flexible electrochemical devices is developed. The nanofibrous hydrogel is self-assembled from guanosine (G) and KB(OH)4 with simultaneous incorporation of hemin into the G-quartet scaffold, giving rise to significant enzyme-like activity...
February 1, 2018: Advanced Materials
Tong Wu, Jialing Zhang, Yuanfei Wang, Binbin Sun, Xuran Guo, Yosry Morsi, Hany El-Hamshary, Mohamed El-Newehy, Xiumei Mo
Simulating the modeling of smooth muscle layer in the vascular structure makes a great difference for vascular tissue regeneration. A functional tissue engineered vascular media shall promote the aligned organization and three-dimensional penetration of smooth muscle cells (SMCs) into the scaffold. To this aim, dynamic liquid and conjugated nanoyarns based on poly(L-lactide-co-caprolactone) (P(LLA-CL)) and collagen (COL) with a weight ratio at 3:1 were fabricated by electrospinning methods, with random and aligned nanofibers as control groups...
March 2017: Journal of Biomedical Nanotechnology
Yoo-Mi Lee, Hyung-Mun Yun, Hye-Young Lee, Hyun-Chang Lim, Hae-Hyoung Lee, Hae-Won Kim, Eun-Cheol Kim
A xerogel was interfaced onto biopolymer nanofibers though a core–shell electrospinning design for bone regeneration. The xerogel-interfaced biopolymer nanofibrous matrix was bioactive and highly hydrophilic, with a significant decrease in the water contact angle. The matrix showed excellent in vitro responses of primary osteoblasts in terms of adhesion, proliferation, and migration. Furthermore, the osteoblastic differentiation of cells, including alkaline phosphatase activity, mineralization, and gene expression, was significantly upregulated by the xerogel interface...
February 2017: Journal of Biomedical Nanotechnology
Gili Kaufman, Ryan A Whitescarver, Laiz Nunes, Xavier-Lewis Palmer, Drago Skrtic, Wojtek Tutak
Deep wounds in the gingiva caused by trauma or surgery require a rapid and robust healing of connective tissues. We propose utilizing gas-brushed nanofibers coated with collagen and fibrin for that purpose. Our hypotheses are that protein-coated nanofibers will: (i) attract and mobilize cells in various spatial orientations, and (ii) regulate the expression levels of specific extracellular matrix (ECM)-associated proteins, determining the initial conformational nature of dense and soft connective tissues. Gingival fibroblast monolayers and 3D spheroids were cultured on ECM substrate and covered with gas-blown poly-(DL-lactide-co-glycolide) (PLGA) nanofibers (uncoated/coated with collagen and fibrin)...
January 24, 2018: Biomedical Materials
Jonathan M Zuidema, Tushar Kumeria, Dokyoung Kim, Jinyoung Kang, Joanna Wang, Geoffrey Hollett, Xuan Zhang, David S Roberts, Nicole Chan, Cari Dowling, Elena Blanco-Suarez, Nicola J Allen, Mark H Tuszynski, Michael J Sailor
Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period...
January 24, 2018: Advanced Materials
Mehrdad Piran, Saeid Vakilian, Mehran Piran, Abdollah Mohammadi-Sangcheshmeh, Simzar Hosseinzadeh, Abdolreza Ardeshirylajimi
Migration of fibroblasts into wound area is a critical phenomenon in wound healing process. We used an appropriate system to fabricate an electrospun bioactive scaffold with controlled release of PDGF-BB in order to induce migration of primary skin fibroblast cells. First of all, protein-loaded chitosan nanoparticles based on ionic gelation interaction between chitosan and sodium tripolyphosphate were prepared. Then polycaprolactone electrospun fibers containing chitosan nanoparticles or PDGF-BB-loaded chitosan nanoparticles were prepared...
January 23, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Seungkuk Ahn, Christophe O Chantre, Alanna R Gannon, Johan U Lind, Patrick H Campbell, Thomas Grevesse, Blakely B O'Connor, Kevin Kit Parker
Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content. More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration. It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen. In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen...
January 23, 2018: Advanced Healthcare Materials
Reza Zamani, Sedigheh Fekri Aval, Younes Pilehvar-Soltanahmadi, Kazem Nejati-Koshki, Nosratollah Zarghami
The progression of nanotechnology provides opportunities to manipulate synthetic and natural materials to mimic the natural structure for tissue engineering applications. The electrospinning technique applies electrostatic principle to fabricate electrospun nanofibers. Nanofiber scaffolds are precisely similar to the native extracellular matrix (ECM) and support cell proliferation, adhesion, tendency to preserve their phenotypic shape and directed growth according to the nanofiber direction. This study reviewed both the natural and synthetic type of nanofibers and described the different properties used to trigger certain process in the tissue development...
January 22, 2018: Drug Research
Sepideh Jalilzadeh-Tabrizi, Younes Pilehvar-Soltanahmadi, Effat Alizadeh, Shahriar Alipour, Mehdi Dadashpour, Kazem Nejati-Koshki, Nosratollah Zarghami
BACKGROUND: Emu oil (EO) with anti-inflammatory, antioxidative, and wound healing properties can be blended for preparing bioactive nanofibrous scaffold. Adipose tissue-derived stem cells (ADSCs) are promising candidates for tissue engineering, and preserving their stemness potential is vital for further therapeutic applications. AIM: The aim of this study was to fabricate EO-blended nanofiber and investigate its effect on proliferation, survival, and stemness preservation of ADSCs...
January 22, 2018: Biopreservation and Biobanking
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