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Nanofiber scaffold

Mariana Laranjeira, Rui M A Domingues, Raquel Costa-Almeida, Rui L Reis, Manuela E Gomes
Tendon and ligament (T/L) function is intrinsically related with their unique hierarchically and anisotropically organized extracellular matrix. Their natural healing capacity is, however, limited. Here, continuous and aligned electrospun nanofiber threads (CANT) based on synthetic/natural polymer blends mechanically reinforced with cellulose nanocrystals are produced to replicate the nanoscale collagen fibrils grouped into microscale collagen fibers that compose the native T/L. CANT are then incrementally assembled into 3D hierarchical scaffolds, resulting in woven constructions, which simultaneously mimic T/L nano-to-macro architecture, nanotopography, and nonlinear biomechanical behavior...
June 20, 2017: Small
Sai Zhang, Guojun Jiang, Molamma P Prabhakaran, Xiaohong Qin, Seeram Ramakrishna
To engineer bone tissue, it is crucial to design scaffolds with micro- and nano-sized architecture imitating approximate hierarchical structure of native bone, and afford desirable biological properties by introducing biocompatible polymers and bioceramics into the scaffolds. Here, a novel scaffold consisting of poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV)/polyaspartic acid (PAA) was fabricated by electrospinning and nano-hydroxyapatite (nHA) was deposited by calcium-phosphate dipping process for bone tissue regeneration...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Aytak Jouybar, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi, Ali Zandi-Karimi, Nikta Feizi, Mohammad-Mehdi Khani, Iraj Pousti
The wound healing process is directly related to the type of treatment. Existing methods of treatment are not responsive enough for severe wounds. The aim of this study was the potential capacity investigation of poly-L-lactic acid (PLLA) nanofibrous scaffolds coated by aloe vera gel for wound dressing applications. In this study, electrospinning method was used for preparing PLLA nanofibers, and after characterization by SEM and MTT, its influence on the wound healing process was investigated with and without aloe vera gel as a wound dressing in full-thickness skin defect in mice...
June 16, 2017: Artificial Organs
Gi-Wan Kwon, Kailash Chandra Gupta, Kyung-Hye Jung, Inn-Kyu Kang
BACKGROUND: To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen-hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1...
2017: Biomaterials Research
Bo Duan, Kangquan Shou, Xiaojuan Su, Yahui Niu, Guan Zheng, Yao Huang, Ai-Xi Yu, Yu Zhang, Hong Xia, Lina Zhang
Chitin exists abundantly in the crab and shrimp shell as the template of the minerals, which inspired us to mineralize it for fabricating the bone grafting materials. In present work, chitin nanofibrous microspheres were used as the matrix for in situ synthesisof the hydroxyapatite (HA) crystals including micro-flake, submicron-needle and submicron-osphere, which were penetrated by long chitin nanofibers, leading to the hierarchical structure. The shape and size of the HA crystals could be controled by changing the HA synthesisprocess...
June 15, 2017: Biomacromolecules
G An, W-B Zhang, D-K Ma, B Lu, G-J Wei, Y Guang, C-H Ru, Y-S Wang
OBJECTIVE: To investigate the influence of VEGF/BMP-2 on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells BMSCs) on PLGA/gelatin composite scaffold. MATERIALS AND METHODS: Randomly-oriented nanofibers with different ratios of Poly Lactic-co-Glycolic Acid (PLGA)/gelatin were produced through electrospinning. The mixture of nanofibers and BMSCs was pipetted onto the surface of the scaffolds, and BMSCs/PLGA/gelatin composite was obtained...
May 2017: European Review for Medical and Pharmacological Sciences
Jun Liang, Gang Liu, Jing Wang, Xiuzhi Susan Sun
Previously, it has been reported that a novel PepGel (h9e peptide) can be triggered into a solid physical hydrogel by the addition of selected ions and proteins for various biomedical applications. Moreover, PepGel displays shear-thinning and repeatedly reversible sol-gel transfer properties that enable it to be easily transferred via an injector. In this study, PepGel is proposed as a carrier for controlled releases of bovine serum albumin (BSA)-bound or -linked drugs. BSA-linked cisplatin (BSA-CP) is used as a model drug in this study and plays two roles: as a trigger of hydrogel and as a target drug for controlled release...
June 12, 2017: Amino Acids
Abdul Jalil Rufaihah, I Ceren Yasa, Vaibavi Srirangam Ramanujam, Suganya Cheyyatraivendran Arularasu, Theo Kofidis, Mustafa O Guler, Ayse B Tekinay
Myocardial infarction remains one of the top leading causes of death in the world and the damage sustained in the heart eventually develops into heart failure. Limited conventional treatment options due to the inability of the myocardium to regenerate after injury and shortage of organ donors require the development of alternative therapies to repair the damaged myocardium. Current efforts in repairing damage after myocardial infarction concentrates on using biologically derived molecules such as growth factors or stem cells, which carry risks of serious side effects including the formation of teratomas...
June 6, 2017: Acta Biomaterialia
Yaobin Wu, Ling Wang, Baolin Guo, Peter X Ma
Mimicking the anisotropic cardiac structure and guiding 3D cellular orientation play a critical role in designing scaffolds for cardiac tissue regeneration. Significant advances have been achieved to control cellular alignment and elongation, but it remains an ongoing challenge for engineering 3D cardiac anisotropy using these approaches. Here, we present a 3D hybrid scaffold based on aligned conductive nanofiber yarns network (NFYs-NET, composition: polycaprolactone, silk fibroin, and carbon nanotubes) within a hydrogel shell for mimicking the native cardiac tissue structure, and further demonstrate their great potential for engineering 3D cardiac anisotropy for cardiac tissue engineering...
June 7, 2017: ACS Nano
Gi Hoon Yang, Hojun Jeon, GeunHyung Kim
For successful skeletal muscle tissue regeneration, inducing alignment and fusion of myoblasts into multinucleated myotubes is critical. Many studies are ongoing to induce myoblast alignment using various micro/nanopatternings on scaffold surfaces, mechanically stretching scaffolds, or aligned micro/nanofibers. In this study, we have developed a simple method to induce myoblast alignment using a modified plasma treatment on a hybrid PCL scaffold consisting of melt-printed perpendicular PCL struts and an electrospun PCL fibrous mat...
June 7, 2017: Biofabrication
Shima Tanha, Morteza Rafiee-Tehrani, Mohamad Abdollahi, Saeid Vakilian, Zahra Esmaili, Zahra Safaei Naraghi, Ehsan Seyedjafari, Hamid Akbari Javar
Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in PCL nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP)HPLC and size-exclusion chromatography (SEC), as well as circular dichroism (CD)...
June 7, 2017: Journal of Biomedical Materials Research. Part A
Bernhard Baumann, Rainer Wittig, Mika Lindén
The incorporation of nanoparticles as drug vectors into 3D scaffolds has attracted a lot of recent interest. In particular, tissue engineering applications would benefit from a spatially and temporally regulated release of biological cues, which act on precursor/stem cells in a three-dimensional growth environment. Injectable cell- and nanoparticle-containing scaffolds are especially interesting in this respect, but require matrix self-assembly and coordinated interactions between cells, matrices, and nanoparticles, which are largely uncharacterized yet...
June 6, 2017: Nanoscale
Benjamin B Rothrauff, Guang Yang, Rocky S Tuan
BACKGROUND: Biological scaffolds composed of tissue-derived extracellular matrix (ECM) can promote homologous (i.e., tissue-specific) cell differentiation through preservation of biophysical and biochemical motifs found in native tissues. Solubilized ECMs derived from decellularized tendon and cartilage have recently been promoted as tissue-specific biomaterials, but whether tissue-specific bioactivity is preserved following solubilization is unknown. This study explored the tissue-specific bioactivity of soluble decellularized tendon and cartilage ECMs on human bone marrow-derived mesenchymal stem cells (MSCs) presented across different culture microenvironments, including two-dimensional (2D) tissue culture plastic, aligned electrospun nanofibers, cell pellets, and cell-seeded photocrosslinkable hydrogels...
June 5, 2017: Stem Cell Research & Therapy
Ekambaram Shoba, Rachita Lakra, Manikantan Syamala Kiran, Purna Sai Korrapati
The physiological and pathological complexity of the wound healing process makes it more challenging to design an ideal tissue regeneration scaffold. Precise scaffolding with high drug loading efficiency, efficient intracellular efficacy for therapeutic delivery, minimal nonspecific cellular and blood protein binding, and maximum biocompatibility forms the basis for an ideal delivery system. This paper describes a combinational multiphasic delivery system, where biomolecules are delivered through the fabrication of coaxial electrospinning of different biocompatible polymers...
June 5, 2017: Biomedical Materials
Haiping Chen, Shikun Xie, Yuanmo Yang, Jing Zhang, Zhuangya Zhang
Biocompatible scaffolds play an important role in modulating tissue growth. A gelatin and sodium alginate scaffold with a unique structure produced by a combination of 3-D printing, electrospinning, and vacuum freeze-drying has been developed for tissue engineering. The scaffold is composed of a macrostructure, a honeycomb microporous surface morphology, and nanofibers. This structure meets the design criteria for an ideal tissue engineering scaffold. The scaffold degrades and has low cytotoxicity. The biocompatibility of the scaffold is improved by the favorable cell-matrix interaction; cells attach to the scaffold well and secrete large amounts of extracellular matrix in vitro...
June 3, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Ali KarbalaeiMahdi, Mohsen Shahrousvand, Hamid Reza Javadi, Marzieh Ghollasi, Faezeh Norouz, Mehdi Kamali, Ali Salimi
In the present study, for the first time, polycaprolactone (PCL) and gelatin (GEL) were used for neural differentiation of human induced pluripotent stem cells (hiPSCs) in the form of bi-electrospun nanofibers. The electrospun fibers were evaluated by FTIR and tensile analysis. MTT assay was used to evaluate the toxicity on the scaffolds. The hiPSCs were seeded on the fibers and after 14days in neural differentiation medium. To confirm the differentiation, real-time PCR and immunocytochemistry (ICC) analyses were performed...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
M Shamsi, M Karimi, M Ghollasi, N Nezafati, M Shahrousvand, M Kamali, A Salimi
Electrospinning method was employed for fabrication of SiO2-CaO-P2O5 bioactive glass (BG) nanofibers, poly-l-lactic acid (PLLA) nanofibers and nanocomposite scaffolds fabricated from as-prepared nanofibers. Characterization of the prepared nanofibers and scaffolds by XRD, FTIR, and SEM techniques revealed the formation of nanofibers with mean diameter of about 500nm and fully fibrous scaffolds with porous structure and interconnected pores. The growth, viability and proliferation of cultured human bone marrow mesenchymal stem cells in the fabricated nanofibers and bioactive glass-poly-l-lactic acid (BG-PLLA) nanocomposite scaffolds were studied using various biological assays including MTT, ALP activity, calcium deposit content, Alizarin red staining, and RT-PCR test...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Liying Xiao, Shanshan Liu, Danyu Yao, Zhaozhao Ding, Zhihai Fan, Qiang Lu, David L Kaplan
Detailed control of nano- and microstructures in porous biomaterial scaffold systems is important for control of interfacial and biological functions. Self-assembled silk protein nanostructured building blocks were incorporated into salt-leached scaffolds to control these features. Controllable concentration and pH were used to induce the formation of amorphous silk nanofibers in solution and also to reduce beta-sheet transformation during the more traditional salt-leaching process. These new scaffolds showed nanofibrous-microporous structures, reduced beta-sheet content, and tunable mechanical properties...
June 2, 2017: Biomacromolecules
Hadi Shirali, Mehdi Rafizadeh, Faramarz Afshar Taromi, Esmaiel Jabbari
A combination of elastic poly(butylene succinate-co-ethylene terephthalate) and rigid nano-hydroxyapatite were used to prepare an in-situ synthesized nanocomposite mimicing bone structure. The microstructure, morphology and dispersion of nanoparticles in the nanocomposites were studied using (1) H-NMR, SEM and TEM, respectively. Then, electrospinning method was used to produce nanofiber matrix with lowest fiber diameter. Presence of chemical bonds among nanoparticles and polymer leads to the excellent particle dispersion and solution phase stability...
June 1, 2017: Journal of Biomedical Materials Research. Part A
Roya Dastjerdi, Mahsa Sharafi, Kourosh Kabiri, Leila Mivehi, A Samadikuchaksaraei
An acid-free water-born chitosan derivative/montmorillonite has been successfully synthesized. A natural based bio-polymer, N-(2-hydroxy) propyl-3-trimethyl ammonium chitosan chloride (HTCC), was synthesized, and its structure confirmed by FTIR and conductometric titration. It was applied to the cationic ion exchange reaction of montmorillonite. Then, the synthesized materials were used to produce water-born composite scaffolds for tissue engineering applications and formed an ultra-fine bead-free multicomponent nanofibrous scaffold...
May 31, 2017: Biomedical Materials
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