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Nanofiber scaffold

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https://www.readbyqxmd.com/read/27934786/electrospun-polycaprolactone-chitosan-scaffolds-for-nerve-tissue-engineering-physicochemical-characterization-and-schwann-cell-biocompatibility
#1
Ena Bolaina-Lorenzo, Cristina Martínez-Ramos, Manuel Monleón-Pradas, Wilberth Herrera-Kao, Juan V Cauich-Rodríguez, José M Cervantes-Uc
Electrospun polycaprolactone (PCL)/chitosan (CH) blend scaffolds with different CH weight ratios were prepared to study the effect of scaffold composition on its physicochemical and biological properties. Scanning electron microscopy showed bead-free homogeneous randomly arranged nanofibers whose average diameter decreased from 240 to 110 nm with increasing CH content. The infrared spectra of the PCL/CH blends were very similar to the neat PCL scaffold. Energy-dispersive x-ray spectroscopy analysis confirmed the presence of carbon, oxygen and nitrogen in the scaffolds, although fluorine-from chemicals used as solvent-was also detected...
December 9, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27931176/in-vitro-evaluation-of-electrospun-silk-fibroin-nano-hydroxyapatite-bmp-2-scaffolds-for-bone-regeneration
#2
Bingjie Niu, Bin Li, Yong Gu, Xiaofeng Shen, Yong Liu, Liang Chen
Bone tissue engineering by using osteoinductive scaffolds seeded with stem cells to promote bone extracellular matrix (ECM) production and remodeling has evolved into a promising approach for bone repair and regeneration. In order to mimic the ECM of bone tissue structurally and compositionally, nanofibrous silk fibroin (SF) scaffolds containing hydroxyapatite (HAP) nanoparticles and bone morphogenetic protein 2 (BMP-2) were fabricated in this study using electrospinning technique. The microstructure, mechanical property, biocompatibility, and osteogenic characteristics were examined...
December 9, 2016: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/27924854/cell-studies-of-hybridized-carbon-nanofibers-containing-bioactive-glass-nanoparticles-using-bone-mesenchymal-stromal-cells
#3
Xiu-Rui Zhang, Xiao-Qing Hu, Xiao-Long Jia, Li-Ka Yang, Qing-Yang Meng, Yuan-Yuan Shi, Zheng-Zheng Zhang, Qing Cai, Yin-Fang Ao, Xiao-Ping Yang
Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofibers (CNF) via the combination of BG sol-gel and polyacrylonitrile (PAN) electrospinning, followed by carbonization. Three types (49 s, 68 s and 86 s) of BG nanoparticles were incorporated. To understand the mechanism of CNF/BG hybrids exerting osteogenic effects, bone marrow mesenchymal stromal cells (BMSCs) were cultured directly on these hybrids (contact culture) or cultured in transwell chambers in the presence of these materials (non-contact culture)...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27923327/formation-of-nanofibrous-matrices-3d-scaffolds-and-microspheres-from-theory-to-practice
#4
Chi Ma, Xiaohua Liu
Nanofibrous architecture presents unique biophysical cues to facilitate cellular responses and is considered an indispensable feature of a biomimetic three-dimensional (3D) scaffold and cell carrier. While electrospinning is a widely used method to prepare natural extracellular matrix-like nanofibers, it faces significant challenges to incorporate nanofibrous architecture into well-defined macroporous 3D scaffolds or injectable microspheres. Here we report a non-electrospinning approach that is generally effective at generating nanofibers from a variety of synthetic and natural biodegradable polymers and integrating these nanofibers into (1) 3D scaffolds with constructive geometry and designed internal macropore structures; and (2) injectable microspheres...
December 6, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27918967/nanoencapsulation-an-efficient-and-promising-approach-to-maximize-wound-healing-efficacy-of-curcumin-a-review-of-new-trends-and-state-of-the-art
#5
REVIEW
Zahid Hussain, Hnin Ei Thu, Shiow-Fern Ng, Shahzeb Khan, Haliza Katas
Wound healing is a multifarious and vibrant process of replacing devitalized and damaged cellular structures, leading to restoration of the skin's barrier function, re-establishment of tissue integrity, and maintenance of the internal homeostasis. Curcumin (CUR) and its analogs have gained widespread recognition due to their remarkable anti-inflammatory, anti-infective, anticancer, immunomodulatory, antioxidant, and wound healing activities. However, their pharmaceutical significance is limited due to inherent hydrophobic nature, poor water solubility, low bioavailability, chemical instability, rapid metabolism and short half-life...
November 30, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27917883/comparison-of-cell-behavior-on-pva-pva-gelatin-electrospun-nanofibers-with-random-and-aligned-configuration
#6
Chen-Yu Huang, Keng-Hsiang Hu, Zung-Hang Wei
Electrospinning technique is able to create nanofibers with specific orientation. Poly(vinyl alcohol) (PVA) have good mechanical stability but poor cell adhesion property due to the low affinity of protein. In this paper, extracellular matrix, gelatin is incorporated into PVA solution to form electrospun PVA-gelatin nanofibers membrane. Both randomly oriented and aligned nanofibers are used to investigate the topography-induced behavior of fibroblasts. Surface morphology of the fibers is studied by optical microscopy and scanning electron microscopy (SEM) coupled with image analysis...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27914018/encapsulation-of-%C3%AE-sitosterol-in-polyurethane-by-sol-gel-electrospinning
#7
Musarat Amina, Touseef Amna, Nawal Al-Musayeib, Sami A Zabin, M Shamshi Hassan, Myung-Seob Khil
Pristine β-sitosterol or in combination with other phytosterols is utilized in an array of enriched commercial foods. Considering the presence of β-sitosterol in different functional foods and its potential role in prevention and cure of neurodegenerative diseases, the aims of our investigation were to encapsulate β-sitosterol in nanofibers and to estimate influence of β-sitosterol on proliferation of fibroblasts. Electrospun nanofibers have widely been used as scaffolds to mimic natural extracellular matrix...
December 2, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27909870/erratum-to-evaluation-of-the-effect-of-nt-3-and-biodegradable-poly-l-lactic-acid-nanofiber-scaffolds-on-differentiation-of-rat-hair-follicle-stem-cells-into-neural-cells-in-vitro
#8
Fatemeh Moghani Ghoroghi, Leila Beygom Hejazian, Banafshe Esmaeilzade, Masumeh Dodel, Masoud Roudbari, Maliheh Nobakht
No abstract text is available yet for this article.
December 1, 2016: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/27907145/training-to-improve-precision-and-accuracy-in-the-measurement-of-fiber-morphology
#9
Nathan A Hotaling, Jun Jeon, Mary Beth Wade, Derek Luong, Xavier-Lewis Palmer, Kapil Bharti, Carl G Simon
An estimated $7.1 billion dollars a year is spent due to irreproducibility in pre-clinical data from errors in data analysis and reporting. Therefore, developing tools to improve measurement comparability is paramount. Recently, an open source tool, DiameterJ, has been deployed for the automated analysis of scanning electron micrographs of fibrous scaffolds designed for tissue engineering applications. DiameterJ performs hundreds to thousands of scaffold fiber diameter measurements from a single micrograph within a few seconds, along with a variety of other scaffold morphological features, which enables a more rigorous and thorough assessment of scaffold properties...
2016: PloS One
https://www.readbyqxmd.com/read/27906586/neural-differentiation-of-mesenchymal-stem-cells-on-scaffolds-for-nerve-tissue-engineering-applications
#10
Kerlin Quintiliano, Thayane Crestani, Davi Silveira, Virginia Etges Helfer, Annelise Rosa, Eduardo Balbueno, Daniela Steffens, Geraldo Pereira Jotz, Diogo André Pilger, Patricia Pranke
Scaffolds produced by electrospinning act as supports for cell proliferation and differentiation, improved through the release of neurotrophic factors. The objective of this study was to develop aligned and random nanofiber scaffolds with and without nerve growth factor to evaluate the potential of mesenchymal stem cells (MSCs) for neural differentiation. Nanofiber morphology, diameter, degradability, cell morphology, adhesion, proliferation, viability, cytotoxicity, and neural differentiation were performed to characterize the scaffolds...
November 2016: Cellular Reprogramming
https://www.readbyqxmd.com/read/27904497/preparation-of-polypyrrole-embedded-electrospun-poly-lactic-acid-nanofibrous-scaffolds-for-nerve-tissue-engineering
#11
Jun-Feng Zhou, Yi-Guo Wang, Liang Cheng, Zhao Wu, Xiao-Dan Sun, Jiang Peng
Polypyrrole (PPy) is a biocompatible polymer with good conductivity. Studies combining PPy with electrospinning have been reported; however, the associated decrease in PPy conductivity has not yet been resolved. We embedded PPy into poly(lactic acid) (PLA) nanofibers via electrospinning and fabricated a PLA/PPy nanofibrous scaffold containing 15% PPy with sustained conductivity and aligned topography. There was good biocompatibility between the scaffold and human umbilical cord mesenchymal stem cells as well as Schwann cells...
October 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27886552/three-dimensional-electrospun-pcl-pla-blend-nanofibrous-scaffolds-with-significantly-improved-stem-cells-osteogenic-differentiation-and-cranial-bone-formation
#12
Qingqing Yao, Jaqueline G L Cosme, Tao Xu, Jacob M Miszuk, Paulo H S Picciani, Hao Fong, Hongli Sun
Nanofibrous scaffolds that are morphologically/structurally similar to natural ECM are highly interested for tissue engineering; however, the electrospinning technique has the difficulty in directly producing clinically relevant 3D nanofibrous scaffolds with desired structural properties. To address this challenge, we have developed an innovative technique of thermally induced nanofiber self-agglomeration (TISA) recently. The aim of this work was to prepare (via the TISA technique) and evaluate 3D electrospun PCL/PLA blend (mass ratio: 4/1) nanofibrous scaffolds having high porosity of ∼95...
January 2017: Biomaterials
https://www.readbyqxmd.com/read/27885831/a-bioinspired-ultraporous-nanofiber-hydrogel-mimic-of-the-cartilage-extracellular-matrix
#13
Florian A Formica, Ece Öztürk, Samuel C Hess, Wendelin J Stark, Katharina Maniura-Weber, Markus Rottmar, Marcy Zenobi-Wong
A true biomimetic of the cartilage extracellular matrix (ECM) could greatly contribute to our ability to regenerate this tissue in a mechanically demanding, often inflamed environment. Articular cartilage is a composite tissue made of cells and fibrillar proteins embedded in a hydrophilic polymeric meshwork. Here, a polyanionic functionalized alginate is used to mimic the glycosaminoglycan component of the native ECM. To create the fibrillar component, cryoelectrospinning of poly(ε-caprolactone) on a -78 °C mandrel, subsequently treated by O2 plasma, is used to create a stable, ultraporous and hydrophillic nanofiber network...
November 25, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27872844/fabrication-of-poly-%C3%AE%C2%B5-caprolactone-scaffolds-reinforced-with-cellulose-nanofibers-with-and-without-the-addition-of-hydroxyapatite-nanoparticles
#14
Pedro Morouço, Sara Biscaia, Tânia Viana, Margarida Franco, Cândida Malça, Artur Mateus, Carla Moura, Frederico C Ferreira, Geoffrey Mitchell, Nuno M Alves
Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues. In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP)...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27865067/laminin-modified-and-aligned-phbv-peo-nanofibrous-nerve-conduits-promote-peripheral-nerve-regeneration
#15
Xiao-Feng Zhang, Hai-Xia Liu, Lazarus Santiago Ortiz, Zhong-Dang Xiao, Ning-Ping Huang
Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) has received much attention for its biodegradability and biocompatibility, characteristics which are required in tissue engineering. In this study, polyethylene oxide (PEO)-incorporated PHBV nanofibers with random or aligned orientation were obtained by electrospinning. For further use in vivo, the nanofiber films were made into nerve conduits after treated with NH3 plasma, which could improve the hydrophilicity of inner surfaces of nerve conduits and then facilitate laminin adsorption via electrostatic interaction for promoting cell adhesion and proliferation...
November 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27863068/scaffold-free-tissue-engineering-of-functional-corneal-stromal-tissue
#16
Fatima N Syed-Picard, Yiqin Du, Andrew J Hertsenberg, Rachelle Palchesko, Martha L Funderburgh, Adam W Feinberg, James L Funderburgh
Blinding corneal scarring is predominately treated with allogeneic graft tissue, however, there is a worldwide shortage of donor tissue leaving millions in need of therapy. Human corneal stromal stem cells (CSSC) have been shown produce corneal tissue when cultured on nanofiber scaffolding, but this tissue cannot be readily separated from the scaffold. In this study, scaffold-free tissue engineering methods were used to generate biomimetic corneal stromal tissue constructs that can be transplanted in vivo without introducing the additional variables associated with exogenous scaffolding...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27861428/hepatogenic-differentiation-of-human-induced-pluripotent-stem-cells-on-collagen-coated-polyethersulfone-nanofibers
#17
Maryam Mahmoodinia Maymand, Hamid Reza Soleimanpour-Lichaei, Abdolreza Ardeshirylajimi, Masoud Soleimani, Samaneh Mirzaei, Athena Hajarizadeh, Maryam Kabir Salmani
Many scientists have been fascinated with Induced pluripotent stem cells (iPSCs) for cell replacement therapies. Nanofibrous biocompatible scaffolds have been shown to promote better cell adhesion and improve stem cell differentiation. In the present study, after fabrication using electrospinning technique and surface modifications, the characteristics of Polyethersulfone (PES) nanofibers were determined by scanning electron microscopy (SEM), ATR-FTIR and MTT assay. Then, the hepatogenic potential of iPSCs was evaluated using Real-Time RT-PCR and immunocytochemistry (ICC) after culture on collagen coated polyethersulfone (PES/COL) scaffolds...
November 15, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/27832002/renal-differentiation-of-mesenchymal-stem-cells-seeded-on-nanofibrous-scaffolds-improved-by-human-renal-tubular-cell-lines-conditioned-medium
#18
Abdolreza Ardeshirylajimi, Saeid Vakilian, Mohammad Salehi
Kidney injuries and renal dysfunctions are one of the most important clinical problems and tissue engineering could be a valuable method for solving it. The objective of this study was to investigate the synergistic effect of renal cell line conditioned medium and Polycaprolactone nanofibers on renal differentiation of human mesenchymal stem cells. In the present study, after stem cells isolation and characterization, Polycaprolactone nanofibrous scaffold was fabricated using electrospinning methods and characterized morphologically, mechanically and biocompatibility...
November 9, 2016: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/27800687/latent-oxidative-polymerization-of-catecholamines-as-potential-crosslinkers-for-biocompatible-and-multifunctional-biopolymer-scaffolds
#19
Chetna Dhand, Veluchamy Amutha Barathi, Seow Theng Ong, Mayandi Venkatesh, Sriram Harini, Neeraj Dwivedi, Eunice Tze Leng Goh, Muruganantham Nandhakumar, Jayarama Reddy Venugopal, Silvia Marrero Diaz, Mobashar Hussain Urf Turabe Fazil, Xian Jun Loh, Liu Shou Ping, Roger W Beuerman, Navin Kumar Verma, Seeram Ramakrishna, Rajamani Lakshminarayanan
Electrospinning of naturally occurring biopolymers for biological applications requires post-spinning crosslinking for endurance in protease-rich microenvironments and prevention of rapid dissolution. The most commonly used crosslinkers often generate cytotoxic byproducts, necessitate high concentrations or time-consuming procedures. Herein, we report the addition of "safe" catecholamine crosslinkers to collagen or gelatin dope solutions followed by electrospinning yielded junction-containing nanofibrous mats...
November 1, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27792283/self-assembled-peptide-based-hydrogels-as-scaffolds-for-proliferation-and-multi-differentiation-of-mesenchymal-stem-cells
#20
Yung-Li Wang, Shih-Pei Lin, Srinivasa Rao Nelli, Fu-Kai Zhan, Hsun Cheng, Tsung-Sheng Lai, Mei-Yu Yeh, Hsin-Chieh Lin, Shih-Chieh Hung
Fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Fmoc-FF) and Fmoc-arginine-glycine--aspartate (Fmoc-RGD) peptides self-assemble to form a 3D network of supramolecular hydrogel (Fmoc-FF/Fmoc-RGD), which provides a nanofibrous network that uniquely presents bioactive ligands at the fiber surface for cell attachment. In the present study, mesenchymal stem cells (MSCs) in Fmoc-FF/Fmoc-RGD hydrogel increase in proliferation and survival compared to those in Fmoc-FF/Fmoc-RGE hydrogel. Moreover, MSCs encapsulated in Fmoc-FF/Fmoc-RGD hydrogel and induced in each defined induction medium undergo in vitro osteogenic, adipogenic, and chondrogenic differentiation...
October 28, 2016: Macromolecular Bioscience
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