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Nanofiber scaffold

Guo Li, Sirong Shi, Shiyu Lin, Tengfei Zhou, Xiaoru Shao, Qiao Huang, Bofeng Zhu, Xiaoxiao Cai
BACKGROUND: Due to its poor self-repair ability, the cartilage injury has been a great challenge in clinical work. OBJECTIVE: The conventional therapies for cartilage injury mostly form fibrocartilage with weaker strength and flexibility compared to natural cartilage. The development of tissue engineering technology provides a new approach for cartilage repair and regeneration. RESULTS: An appropriate scaffold is one of key factors in cartilage tissue engineering...
April 17, 2018: Current Stem Cell Research & Therapy
Yang Huang, Jinsong Kong, Xiaokang Gong, Xin Zheng, Haibao Wang, Jianwei Ruan
<b>Objective:</b> To analysis the biomechanical and biocompatible properties of calcium phosphate cement (CPC) enhanced by chitosan short nanofibers(CSNF) and Arg-Gly-Asp (RGD). <b>Methods:</b> Chitosan nanofibers were prepared by electrospinning, and cut into short fibers by high speed dispersion. CPC with calcium phosphorus ratio of 1.5:1 was prepared by Biocement D method. The composition and structure of CPC, CSNF, RGD modified CSNF (CSNF-RGD), CSNF enhanced CPC (CPC-CSNF), RGD modified CPC-CSNF (CPC-CSNF-RGD) were observed by infrared spectrum, X-ray diffraction (XRD) and scan electron microscopy (SEM)...
May 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Afarin Karimi, Saeed Karbasi, Shahnaz Razavi, Elham Naghash Zargar
Background: Reconstruction of nervous system is a great challenge in the therapeutic medical field. Nerve tissue engineering is a novel method to regenerate nervous system in human health care. Tissue engineering has introduced novel approaches to promote and guide peripheral nerve regeneration using submicron and nanoscale fibrous scaffolds. Materials and Methods: In this study, 9 wt% poly(3-hydroxybutyrate) (PHB) solutions with two different ratios of chitosan (CTS) (15%, and 20%) were mixed in trifluoroacetic acid as a cosolvent...
2018: Advanced Biomedical Research
Elif Arslan, Melis Sardan Ekiz, Cagla Eren Cimenci, Nuray Can, M Hanifi Gemci, Huseyin Ozkan, Mustafa O Guler, Ayse B Tekinay
Osteoarthritis (OA) is a condition where tissue function is lost through a combination of secondary inflammation and deterioration in articular cartilage. One of the most common causes of OA is age-related tissue impairment because of wear and tear due to mechanical erosion. Hyaluronic acid-based viscoelastic supplements have been widely used for the treatment of knee injuries. However, the current formulations of hyaluronic acid are unable to provide efficient healing and recovery. Here, a nanofiber-hyaluronic acid membrane system that was prepared by using a quarter of the concentration of commercially available hyaluronic acid supplement, Hyalgan® , was used for the treatment of an osteoarthritis model, and Synvisc® , which is another commercially available hyaluronic acid containing viscoelastic supplement, was used as a control...
April 12, 2018: Acta Biomaterialia
Priya Vashisth, Jayesh R Bellare
In the present study, a biomimetic three-dimensional hybrid scaffold has been designed considering the bone natural architecture with favorable interconnected porous structure, nano-microscale features and mechanical strength. The chief components of the hybrid scaffold are core-sheath nanofibers and hydrogel, suitably arranged to create a bone like microenvironment. Specifically, the core-sheath nanofibers were coiled tightly into a ring to mimic the osteon, and reinforced in a hydrogel matrix. Morphological analysis using SEM and 4D-X-ray microscopy revealed that the hybrid scaffold consists of coiled rings of nanofibers in highly porous hydrogel matrix showing structural similarity to osteons...
April 9, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Nathalie Barroca, Ana Marote, Sandra I Vieira, Abílio Almeida, Maria H V Fernandes, Paula M Vilarinho, Odete A B da Cruz E Silva
Tissue engineering is evolving towards the production of smart platforms exhibiting stimulatory cues to guide tissue regeneration. This work explores the benefits of electrical polarization to produce more efficient neural tissue engineering platforms. Poly (l-lactic) acid (PLLA)-based scaffolds were prepared as solvent cast films and electrospun aligned nanofibers, and electrically polarized by an in-lab built corona poling device. The characterization of the platforms by thermally stimulated depolarization currents reveals a polarization of 60 × 10-10 C cm-2 that is stable on poled electrospun nanofibers for up to 6 months...
March 30, 2018: Colloids and Surfaces. B, Biointerfaces
Amin Sadeghi, Mohamad Pezeshki-Modaress, Mojgan Zandi
Electrospun nanofibers have attracted a lot of attention in recent years in tissue engineering applications. In this research, novel polyvinyl alcohol/gelatin/chondroitin sulfate (PVA/GE/Cs) nanofibrous scaffolds using non-carcinogen solvent system via electrospinning technique was evaluated. A solvent system containing water and acetic acid was used as a safe solvent system to obtain a homogenous mixture with suitable solvent properties and finally non-toxic nanofibrous scaffolds. The effect of water to the acetic acid ratio in the solvent system (7:3, 6:4, 5:5, 4:6, 3:7) and also polymer concentration (8, 9, 10 w/v %) on nanofibers morphology was investigated...
April 5, 2018: International Journal of Biological Macromolecules
Payam Zarrintaj, Behnaz Bakhshandeh, Mohammad Reza Saeb, Farshid Sefat, Iraj Rezaeian, Mohammad Reza Ganjali, Seeram Ramakrishna, Masoud Mozafari
The science and engineering of biomaterials have improved the human life expectancy. Tissue engineering is one of the nascent strategies with an aim to fulfill this target. Tissue engineering scaffolds are one of the most significant aspects of the recent tissue repair strategies; hence, it is imperative to design biomimetic substrates with suitable features. Conductive substrates can ameliorate the cellular activity through enhancement of cellular signaling. Biocompatible polymers with conductivity can mimic the cells' niche in an appropriate manner...
April 3, 2018: Acta Biomaterialia
Saeid Vakilian, Mohammad Norouzi, Mahsa Soufi-Zomorrod, Iman Shabani, Simzar Hosseinzadeh, Masoud Soleimani
Since ancient times, some herbal medicines have been extensively used for burn and wound treatments, showing preference to the common synthetic medications by virtue of having less side effects and faster healing rate. In this study, hybrid nanofibrous scaffolds of poly-l-lactic-acid (PLLA) and gelatin incorporated L. inermis were fabricated via electrospinning technique. Morphology and characteristics of the scaffolds were studied by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR), respectively...
April 2018: Tissue & Cell
Tony T Yuan, Ann Marie DiGeorge Foushee, Monica C Johnson, Angela R Jockheck-Clark, Jonathan M Stahl
Normal wound healing is a highly complex process that requires the interplay of various growth factors and cell types. Despite advancements in biomaterials, only a few bioactive wound dressings reach the clinical setting. The purpose of this research was to explore the feasibility of electrospinning a novel nanofibrous chitosan (CS)-fibrinogen (Fb) scaffold capable of sustained release of platelet-derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. CS-Fb scaffolds were successfully electrospun using a dual-spinneret electrospinner and directly evaluated for their physical, chemical, and biological characteristics...
April 2, 2018: Nanoscale Research Letters
Daniela Steffens, Daikelly I Braghirolli, Natasha Maurmann, Patricia Pranke
Regenerative medicine involves the study of cells, signaling cues and biomatrices to restore normal function of tissues and organs. To develop the matrices for use in tissue engineering there are three main groups of biomaterials: (i) naturally derived materials; (ii) synthetic polymers; and (iii) decellularized organ or tissue scaffolds. These biomaterials, in various forms such as hydrogels, nanofibers and 3D scaffolds, among others, have been employed for different tissue regeneration purposes, with several techniques involved in their production, including rapid prototyping, tissue decellularization and electrospinning...
March 30, 2018: Drug Discovery Today
Lauren Sfakis, Tim Kamaldinov, Alexander Khmaladze, Zeinab F Hosseini, Deirdre A Nelson, Melinda Larsen, James Castracane
Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren's syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds...
March 29, 2018: International Journal of Molecular Sciences
Punamshree Das, Nomita Ojah, Raghuram Kandimalla, Kiranjyoti Mohan, Dolly Gogoi, Swapan Kumar Dolui, Arup Jyoti Choudhury
In this paper, surface of electrospun PVA/Cs nanofibers is modified using dielectric barrier discharge (DBD) plasma and the relationship between the observed mechanical properties and biocompatibility of the nanofibers and plasma-induced surface properties is discussed. Plasma treatment of electrospun PVA/Cs nanofibers is carried out with both inert (argon, Ar) and reactive (oxygen, O2 ) gases at atmospheric pressure. Incorporation of oxygen-containing polar functional groups on the surface of Ar-plasma treated (PVA/Cs/Ar) and O2 -plasma treated (PVA/Cs/O2 ) nanofibers and increase in surface roughness contribute to the improvement of surface wettability and the decrease of contact angle with water of the nanofibers...
March 22, 2018: International Journal of Biological Macromolecules
Jacob M Miszuk, Tao Xu, Qingqing Yao, Fang Fang, Josh D Childs, Zhongkui Hong, Jianning Tao, Hao Fong, Hongli Sun
Bone morphogenic protein 2 (BMP2) is a key growth factor for bone regeneration, possessing FDA approval for orthopedic applications. BMP2 is often required in supratherapeutic doses clinically, yielding adverse side effects and substantial treatment costs. Considering the crucial role of materials for BMPs delivery and cell osteogenic differentiation, we devote to engineering an innovative bone-matrix mimicking niche to improve low dose of BMP2-induced bone formation. Our previous work describes a novel technique, named thermally induced nanofiber self-agglomeration (TISA), for generating 3D electrospun nanofibrous (NF) polycaprolactone (PCL) scaffolds...
March 2018: Applied Materials Today
Yong Cheol Shin, Chuntae Kim, Su-Jin Song, Seungwon Jun, Chang-Seok Kim, Suck Won Hong, Suong-Hyu Hyon, Dong-Wook Han, Jin-Woo Oh
Recently, there have been tremendous efforts to develop the biofunctional scaffolds by incorporating various biochemical factors. In the present study, we fabricated poly(lactic- co -glycolic acid) (PLGA) nanofiber sheets decorated with graphene oxide (GO) and RGD peptide. The decoration of GO and RGD peptide was readily achieved by using RGD peptide-displaying M13 bacteriophage (RGD-M13 phage) and electrospinning. Furthermore, the aligned GO-decorated PLGA/RGD peptide (GO-PLGA/RGD) ternary nanofiber sheets were prepared by magnetic field-assisted electrospinning, and their potentials as bifunctional scaffolds for facilitating myogenesis were explored...
2018: Nanotheranostics
Batool Hashemi-Beni, Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Abbas Ali Khademi
Background: The aim of this study was to compare the cytotoxicity and the biocompatibility of three different nanofibers scaffolds after seeding of stem cells harvested from human deciduous dental pulp. Given the importance of scaffold and its features in tissue engineering, this study demonstrated the construction of polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite scaffold using electrospinning method. Materials and Methods: This experimental study was conducted on normal exfoliated deciduous incisors obtained from 6-year-old to 11-year-old healthy children...
March 2018: Dental Research Journal
Richard P Tan, Alex H P Chan, Katarina Lennartsson, Maria M Miravet, Bob S L Lee, Jelena Rnjak-Kovacina, Zoe E Clayton, John P Cooke, Martin K C Ng, Sanjay Patel, Steven G Wise
BACKGROUND: Induced pluripotent stem-cell derived endothelial cells (iPSC-ECs) can be generated from any somatic cell and their iPSC sources possess unlimited self-renewal. Previous demonstration of their proangiogenic activity makes them a promising cell type for treatment of ischemic injury. As with many other stem cell approaches, the low rate of in-vivo survival has been a major limitation to the efficacy of iPSC-ECs to date. In this study, we aimed to increase the in-vivo lifetime of iPSC-ECs by culturing them on electrospun polycaprolactone (PCL)/gelatin scaffolds, before quantifying the subsequent impact on their proangiogenic function...
March 21, 2018: Stem Cell Research & Therapy
José Luis Suarez-Franco, Febe Carolina Vázquez-Vázquez, Amaury Pozos-Guillen, Juan José Montesinos, Octavio Alvarez-Fregoso, Marco Antonio Alvarez-Perez
This study evaluated the influence in the biocompatibility of human periodontal ligament (hPDL) mesenchymal stromal cell onto poly lactic-acid (PLA) films and PLA fiber membrane. Fiber scaffold was prepared via air jet spinning (AJS) from PLA solutions (6, 7, and 10%) and analyzed using SEM, AFM and FTIR. Biocompatibility was evaluated by adhesion, proliferation and cell-material interaction. PLA film exhibited a smooth and homogenously surface topography in comparison with random orientation of PLA fiber with roughness structure where diameter size depends on PLA solution...
March 17, 2018: Dental Materials Journal
Hande Nalvuran, Ayşe Eser Elçin, Yaşar Murat Elçin
Graphene and silk fibroin (SF) have been extensively investigated in the literature. Hybrid scaffolds of SF and graphene combine the properties of both of the materials and provide promising applications for tissue engineering purposes. In this study, reduced graphene oxide (RGO) (0.5%, 1.0% and 2.0% (w/v)) was incorporated into SF and fabricated into composite nanofibers through electrospinning. The fibers were characterized and analyzed by SEM, XRD, FTIR, TGA, circular dichroism analysis, contact angle measurements and tensile tests...
March 15, 2018: International Journal of Biological Macromolecules
Christophe O Chantre, Patrick H Campbell, Holly M Golecki, Adrian T Buganza, Andrew K Capulli, Leila F Deravi, Stephanie Dauth, Sean P Sheehy, Jeffrey A Paten, Karl Gledhill, Yanne S Doucet, Hasan E Abaci, Seungkuk Ahn, Benjamin D Pope, Jeffrey W Ruberti, Simon P Hoerstrup, Angela M Christiano, Kevin Kit Parker
Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (∼1...
March 5, 2018: Biomaterials
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