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Nanofiber scaffold

Hitomi Nakao, Robin D Jacquet, Mark Shasti, Noritaka Isogai, Ananth S Murthy, William J Landis
BACKGROUND: Previous regeneration studies of auricle-shaped cartilage by tissue engineering leave unresolved whether the chondrocyte phenotype from human auricular chondrocytes seeded onto polymeric scaffolds is retained over the long term and whether microtia remnants may be a viable cell source for auricular reconstruction. METHODS: Chondrocytes were isolated from human ears, either normal conchal ear or microtia cartilage remnants, expanded in vitro, and seeded onto nanoscale-diameter polyglycolic acid sheets...
April 2017: Plastic and Reconstructive Surgery
Duo Wu, Archana Samanta, Rajiv K Srivastava, Minna Hakkarainen
A straightforward process that enabled electrospinning of bioactive starch-based nanofiber scaffolds was developed by utilizing starch derived nano graphene oxide (nGO) as a property enhancer and formic acid as a solvent and esterification reagent. The reaction mechanism and process were followed by detailed spectroscopic investigation. Furthermore, the incorporation of nGO as a "green bioactive additive" endorsed starch nanofibrous scaffolds several advantageous functionalities including improved electrospinnability and thermal stability, good cytocompatibility, osteo-bioactivity, and retained biodegradability...
March 28, 2017: Biomacromolecules
Vahid Zijah, Roya Salehi, Marziyeh Aghazadeh, Mohammad Samiei, Effat Alizadeh, Soodabeh Davaran
Tissue engineering has emerged as a potential therapeutic option for dental problems in recent years. One of the policies in tissue engineering is to use both scaffolds and additive factors for enhancing cell responses. This study aims to evaluate and compare the effect of three types of biofactors on poly-caprolactone-poly-ethylene glycol-poly caprolactone (PCL-PEG-PCL) nanofibrous scaffold on human dental pulp stem cell (hDPSCs) engineering. The PCL-PEG-PCL copolymer was synthesized with ring opening polymerization method, and its nanofiber scaffold was prepared by electrospinning method...
March 25, 2017: In Vitro Cellular & Developmental Biology. Animal
Sarah L Stanley, Frank Scholle, Jiadeng Zhu, Yao Lu, Xiangwu Zhang, Xingci Situ, Reza A Ghiladi
Toward the objective of developing platform technologies for anti-infective materials based upon photodynamic inactivation, we employed electrospinning to prepare a non-woven textile comprised of polyacrylonitrile nanofibers embedded with a porphyrin-based cationic photosensitizer; termed PAN-Por((+)). Photosensitizer loading was determined to be 34.8 nmol/mg material; with thermostability to 300 °C. Antibacterial efficacy was evaluated against four bacteria belonging to the ESKAPE family of pathogens (Staphylococcus aureus; vancomycin-resistant Enterococcus faecium; Acinetobacter baumannii; and Klebsiella pneumonia), as well as Escherichia coli...
April 20, 2016: Nanomaterials
Li Kun, Zhao Yanhong, Xu Chen, Wang Lianyong, Yang Qiang, Li Hongfa, Teng Binhong
OBJECTIVE: This study aimed to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and to investigate their physicochemical property and compatibility with adipose-derived stem cells (ADSCs). METHODS: A fresh porcine articular cartilage was cut into pieces. Cartilage nanofibers with diameters of 50-500 nm were collected through homogenization and centrifugation. These nanofibers were then decellularized by using Triton X-100 to produce 6% CECM...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Forouzan Mohamadi, Somayeh Ebrahimi-Barough, Mohammad Reza Nourani, Mohammad Ali Derakhshan, Vahabodin Goodarzi, Mohammad Sadegh Nazockdast, Mehdi Farokhi, Roksana Tajerian, Reza Faridi Majidi, Jafar Ai
In among of various methods, nerve tissue engineering (NTE) is one of the applicable methods to reconstruct damaged nerve tissues. Electrospinning technique and biomaterials are often considered to fabricate fibrous tissue engineered conduits which have great similarity to the extracellular matrix on fiber structure. Polymer blending is one of the most effective methods for production of new materials with outstanding features. In this research, conduit structures as main part of the peripheral nerve regeneration based on polymer blend nanocomposites PCL/Collagen/NBG (poly (ε-caprolactone)/Collagen/nano bioglass) were manufactured by electrospinning technique...
March 21, 2017: Journal of Biomedical Materials Research. Part A
Anu Hyysalo, Mervi Ristola, Tiina Joki, Mari Honkanen, Minnamari Vippola, Susanna Narkilahti
Stem cell transplantations for spinal cord injury (SCI) have been studied extensively for the past decade in order to replace the damaged tissue with human pluripotent stem cell (hPSC)-derived neural cells. Transplanted cells may, however, benefit from supporting and guiding structures or scaffolds in order to remain viable and integrate into the host tissue. Biomaterials can be used as supporting scaffolds, as they mimic the characteristics of the natural cellular environment. In this study, hPSC-derived neurons, astrocytes, and oligodendrocyte precursor cells (OPCs) are cultured on aligned poly(ε-caprolactone) nanofiber platforms, which guide cell orientation to resemble that of spinal cord in vivo...
March 15, 2017: Macromolecular Bioscience
Yingke Wu, Weiwei Lin, Hongye Hao, Jiehua Li, Feng Luo, Hong Tan
A series of nanofibrous scaffolds, free of organic solvents, are prepared by electrospinning biodegradable waterborne polyurethane (BWPU) emulsion blending with aqueous poly(vinyl alcohol)(PVA). Tuning the proration of BWPU to PVA, various nanofibers with diameter from 370 to 964 nm are obtained. Strong intermolecular interaction existing between them benefits to the electrospun of BWPU emulsion, which is demonstrated by dynamic thermomechanical analysis and Fourier transform infrared spectroscopy. The nontoxic nanofibrous scaffolds with porous structure, which is similar to the natural extracellular matrix, favor to the attachment and proliferation of the L929 fibroblasts...
May 2017: Journal of Biomaterials Science. Polymer Edition
Hsin-Yi Lin, Wen-Chi Tsai, Shih-Hsing Chang
Researchers have made bi-layered scaffolds but mostly for osteochondral repairs. The anatomic structure of human cartilage has different zones and that each has varying matrix morphology and mechanical properties is often overlooked. Two bi-layered collagen-based composites were made to replicate the superficial and transitional zones of an articular cartilage. Aligned and random collagen-PVA nanofibers were electrospun onto a freeze-dried collagen sponge to make the aligned and random composites, respectively...
May 2017: Journal of Biomaterials Science. Polymer Edition
Bing-Jiao Zhao, Yao Xu, Yue-Hua Liu
PURPOSE: To prepare electrospun simvastatin/polycaprolactone(SMV/PCL) membrane scaffolds and to evaluate the release properties of this formulation. METHODS: Electrospun SMV/PCL membrane scaffolds were prepared as the experimental group, and electrospun PCL membrane as the control group. The morphology and characteristics of membrane surface were determined by scanning electron microscopic(SEM) and X-ray diffraction(XRD). The release profile of SMV was determined using an ultraviolet-visible spectrometer...
December 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
Nosratollah Zarghami, Roghayeh Sheervalilou, Amir Fattahi, Abbas Mohajeri, Mehdi Dadashpour, Younes Pilehvar-Soltanahmadi
Conventional dressings are cost-effective and highly absorbent, but not effectual enough to promote hemostasis, adherence and in holding a moist wound bed. Thanks to the developments in the field of nanotechnology and bioengineering, one of the promising current trends is to move progress of innovative wound dressings, merging the application of traditional healing agents and modern products/practices, such as hydrocolloids, hydrogels, films and nanofibers. The electrospun nanofibers webs can provide the essential parameters require for wound dressing to heal wounds including absorptivity, oxygen permeability, and non-adherence to the healing tissue, barrier to bacteria, bioactivity and occlusivity...
March 8, 2017: Mini Reviews in Medicinal Chemistry
Lauren Sfakis, Anna Sharikova, David Tuschel, Felipe Xavier Costa, Melinda Larsen, Alexander Khmaladze, James Castracane
Core/shell nanofibers are becoming increasingly popular for applications in tissue engineering. Nanofibers alone provide surface topography and increased surface area that promote cellular attachment; however, core/shell nanofibers provide the versatility of incorporating two materials with different properties into one. Such synthetic materials can provide the mechanical and degradation properties required to make a construct that mimics in vivo tissue. Many variations of these fibers can be produced. The challenge lies in the ability to characterize and quantify these nanofibers post fabrication...
February 1, 2017: Biomedical Optics Express
Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada
The nanofibres created by electrospinning technique are currently used for a variety of applications in tissue engineering; and Gelatin and Polyurethane Shape-Memory (SMPU) have important results in biomedicine. Similarly, carbon nanotubes combined with other biomaterials change important properties, opening new opportunities for biomedical applications. In this work, we constructed scaffold using electrospinning technique based in bovine-hide gelatin, SMPU and both materials hybrid with carbon nanotube. Morphology and cytotoxicity were evaluated and mechanical properties for two materials were obtained in scaffold building...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Ting Liu, Pindong Li, Honglin Jin, Qian Ding, Zhenwei Zou, Gang Peng
Nanotechnology provides a novel scope for cancer therapeutics. Nanoscale platforms could influence the survival, adhesion and migration of cancer cells through altering the tumor microenvironments. We report here the development of an anti-tumor material designed specifically for cancer therapy. We synthesized a 48-amino acid peptide RADA-KLA, which could self-assemble into nanostructures with antitumor activities. We tested the microstructure of RADA-KLA nanofiber scaffold using transmission electron microscopy (TEM) and accessed rheological properties of the material...
March 6, 2017: Journal of Biomedical Materials Research. Part A
Kuihua Zhang, Dianwu Huang, Zhiyong Yan, Chunyang Wang
Biomimicing topological structure of natural nerve tissue to direct axon growth and controlling sustained release of moderate neurotrophic factors are extremely propitious to the functional recovery of damaged nervous systems. In this study, the heparin/collagen encapsulating nerve growth factor (NGF) multilayers were coated onto the aligned poly-L-lactide (PLLA) nanofibrous scaffolds via a layer-by-layer (LbL) self-assembly technique to combine biomolecular signals and physical guidance cues for peripheral nerve regeneration...
March 3, 2017: Journal of Biomedical Materials Research. Part A
Asma Sadat Motamedi, Hamid Mirzadeh, Fereshteh Hajiesmaeilbaigi, Shadab Bagheri Khoulenjani, Mohammad Ali Shokrgozar
In this study, gold nanoparticles/Polyvinylidenefluoride composite electrospun mat with enhanced piezoelectricity were fabricated and characterized. Gold colloidal nanoparticles (Au NPs) were prepared via laser ablation of metallic targets in liquid media. The active Q-switched Nd:YAG laser was used as an irradiation source. Then, PVDF (Polyvinylidenefluoride) was dissolved in Au NPs colloidal solution at 30% wt for the synthesis of Au NPs/PVDF composite nanofibers by electrospinning. The optical absorbance spectra of Au NPS and the polymeric solutions were obtained by the UV-Visible spectroscopy...
March 3, 2017: Journal of Biomedical Materials Research. Part A
Tingxiao Li, Xin Ding, Lingling Tian, Jiyong Hu, Xudong Yang, Seeram Ramakrishna
Bead-on-string nanofibers, with appropriate control of the beads diameter, are potential fibrous structures for efficient encapsulation of particle drugs in micron scales and could achieve controlled drug release for tissue engineering applications. In this study, the beads diameter of electrospun bead-on-string nanofibers was controlled by adjusting the concentration of spinning polymer, poly (lactic-co-glycolic acid) (PLGA), and the solvent ratio of chloroform to acetone. The images of the scanning electron microscopy (SEM) suggested that bead-on-string nanofibers could be successfully obtained only with a certain range of PLGA solution concentration...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Bin He, Yunsheng Ou, Shuo Chen, Weikang Zhao, Ao Zhou, Jinqiu Zhao, Hong Li, Dianming Jiang, Yong Zhu
d-Form and l-form peptide nanofiber scaffolds can spontaneously form stable β-sheet secondary structures and nanofiber hydrogel scaffolds, and hold some promise in hemostasis and wound healing. We report here on the synthetic self-assembling peptide d-RADA16 and l-RADA16 are both found to produce stable β-sheet secondary structure and nanofiber hydrogel scaffolds based on circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM) and rheology analysis etc. d-RADA16 hydrogel and l-RADA16 hydrogel can enhance obvious bone repair in femoral condyle defects of the Sprague-Dawley (SD) rat model compared to PBS treatment...
May 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
R Witt, A Weigand, A M Boos, A Cai, D Dippold, A R Boccaccini, D W Schubert, M Hardt, C Lange, A Arkudas, R E Horch, J P Beier
BACKGROUND: Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding skeletal muscle cells on aligned constructs enhance the formation of myotubes as well as cell alignment and may provide a further step towards the clinical application of engineered skeletal muscle...
February 28, 2017: BMC Cell Biology
Ohan S Manoukian, Rita Matta, Justin Letendre, Paige Collins, Augustus D Mazzocca, Sangamesh G Kumbar
Electrospinning has emerged as a simple, elegant, and scalable technique that can be used to fabricate polymeric nanofibers. Pure polymers as well as blends and composites of both natural and synthetic ones have been successfully electrospun into nanofiber matrices for many biomedical applications. Tissue-engineered medical implants, such as polymeric nanofiber scaffolds, are potential alternatives to autografts and allografts, which are short in supply and carry risks of disease transmission. These scaffolds have been used to engineer various soft tissues, including connective tissues, such as skin, ligament, and tendon, as well as nonconnective ones, such as vascular, muscle, and neural tissue...
2017: Methods in Molecular Biology
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