keyword
MENU ▼
Read by QxMD icon Read
search

Sperm analysis

keyword
https://www.readbyqxmd.com/read/28737486/-no-daddy-a-kind-of-daddy-words-used-by-donor-conceived-children-and-aspiring-parents-to-refer-to-the-sperm-donor
#1
Veerle Provoost, Jodie Bernaerdt, Hanna Van Parys, Ann Buysse, Petra De Sutter, Guido Pennings
Research has shown that the recipients of donor sperm can experience difficulties finding appropriate language to refer to the donor. Based on two qualitative analysis techniques, namely word count and empirical discourse analysis, we studied the words used to refer to the donor in heterosexual and lesbian (aspiring) parents and in donor conceived children. Findings show that the words used in these households are highly diverse and have at least four different interlinked functions: (1) to position the donor in relation to the nuclear family; (2) to safeguard the role of the social parent; (3) to clarify family structure; and (4) to present a positive picture of the donor...
July 24, 2017: Culture, Health & Sexuality
https://www.readbyqxmd.com/read/28732331/an-evaluation-of-dna-damage-in-human-lymphocytes-and-sperm-exposed-to-methyl-methanesulfonate-involving-the-regulation-pathways-associated-with-apoptosis
#2
Khaled Habas, Mojgan Najafzadeh, Adolf Baumgartner, Martin H Brinkworth, Diana Anderson
Exposure to DNA-damaging agents produces a range of stress-related responses. These change the expression of genes leading to mutations that cause cell cycle arrest, induction of apoptosis and cancer. We have examined the contribution of haploid and diploid DNA damage and genes involved in the regulation of the apoptotic process associated with exposure, The Comet assay was used to detect DNA damage and quantitative RT-PCR analysis (qPCR) to detect gene expression changes in lymphocytes and sperm in response to methyl methanesulfonate...
June 23, 2017: Chemosphere
https://www.readbyqxmd.com/read/28732188/adapted-methods-for-scanning-electron-microscopy-in-assessment-of-human-sperm-morphology
#3
Petra Nussdorfer, Ines Cilenšek, Branko Zorn, Daniel Petrovič
Infertility is a widespread problem, and in some cases, the routine basic semen analysis is not sufficient to detect the cause of male infertility. The use of the scanning electron microscope (SEM) could provide a detailed insight into spermatozoa morphology, but it requires specific sample preparation techniques. The purpose of this study was to select, adjust, and optimize a method for the preparation of spermatozoa samples prior to SEM analysis, and to establish the protocol required for its use in clinical practice...
July 21, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28732140/fibroblast-growth-factor-2-fgf2-is-present-in-human-spermatozoa-and-is-related-with-sperm-motility-the-use-of-recombinant-fgf2-to-improve-motile-sperm-recovery
#4
D J Garbarino Azúa, L Saucedo, S Giordana, M L Magri, M G Buffone, F Neuspiller, M H Vazquez-Levin, C I Marín-Briggiler
Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts...
July 21, 2017: Andrology
https://www.readbyqxmd.com/read/28728862/seminal-plasma-proteins-modify-the-distribution-of-sperm-subpopulations-in-cryopreserved-semen-of-rams-with-lesser-fertility
#5
Alba Ledesma, Lucía Zalazar, Estela Fernández-Alegre, Federico Hozbor, Andreina Cesari, Felipe Martínez-Pastor
Any physiological mechanism involved in sperm selection and semen improvement has effects on heterogeneous sperm populations. This is mainly due to the fact that sperm populations within a single ejaculate have considerable heterogeneity for many variables, such as motility which is meaningful in terms of understanding how some sperm cells possess fertility advantages as compared with other cells. In the present research, initially there was a multivariate and clustering analysis used to assess sperm motility data from cryopreserved ram semen to identify subpopulations and compare the distribution of these clusters between rams with lesser and greater fertility...
June 27, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28727099/identification-of-differentially-expressed-proteins-in-testicular-semen-of-sex-reversed-female-xx-and-normal-male-xy-rainbow-trout
#6
J Nynca, M Adamek, A Ciereszko
Masculinized females, named sex-reversed females (SRF), have a male phenotype but retain the female genotype (XX) and all spermatozoa produced in their testes carry the X chromosome. Masculinization of females leads to incomplete testicular development and the production of lower-quality semen. The mechanism of masculinization is unknown at present. Therefore, the aim of our study was to identify differentially abundant proteins in testicular semen of normal males and SRF using a difference in-gel electrophoresis approach...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727006/genetic-analysis-of-semen-production-traits-of-japanese-black-and-holstein-bulls-genome-wide-marker-based-estimation-of-genetic-parameters-and-environmental-effect-trends
#7
Y Atagi, A Onogi, M Kinukawa, A Ogino, K Kurogi, K Uchiyama, T Yasumori, K Adachi, K Togashi, H Iwata
The semen production traits of bulls from 2 major cattle breeds in Japan, Holstein and Japanese Black, were analyzed comprehensively using genome-wide markers. Weaker genetic correlations were observed between the 2 age groups (1 to 3 yr old and 4 to 6 yr old) regarding semen volume and sperm motility compared with those observed for sperm number and motility after freeze-thawing. The preselection of collected semen for freezing had a limited effect. Given the increasing importance of bull proofs at a young age because of genomic selection and the results from preliminary studies, we used a multiple-trait model that included motility after freeze-thawing with records collected at young ages...
May 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726302/a-multicenter-study-to-evaluate-oxidative-stress-by-oxidation-reduction-potential-a-reliable-and-reproducible-method
#8
A Agarwal, M Arafa, R Chandrakumar, A Majzoub, S AlSaid, H Elbardisi
Seminal oxidative stress (OS) is well-known to affect male fertility status. The discrepancy in OS measurement has hindered its clinical use as a quality indicator for semen. Some tests measured single markers of oxidants or reductants, leading to lack of standardization of results. Oxidation-reduction potential (ORP) is a better representative for OS as it provides an overall measure of the activity of both oxidants and reductants. ORP assessment by MiOXSYS has been introduced as a measure of OS with high specificity in differentiating fertile from infertile semen samples...
July 20, 2017: Andrology
https://www.readbyqxmd.com/read/28725387/origins-of-two-hemiclonal-hybrids-among-three-hexagrammos-species-teleostei-hexagrammidae-genetic-diversification-through-host-switching
#9
Hiroyuki Munehara, Miho Horita, Motoko R Kimura-Kawaguchi, Aya Yamazaki
Two natural, hemiclonal hybrid strains were discovered in three Hexagrammos species. The natural hybrids, all of which were females that produced haploid eggs containing only the Hexagrammos octogrammus genome (maternal ancestor; hereafter Hoc), generated F1 hybrid-type offspring by fertilization with haploid sperm of Hexagrammos agrammus or Hexagrammos otakii (paternal species; Hag and Hot, respectively). This study was performed to clarify the extent of diversification between the two hybrids and the maternal ancestor...
October 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/28724632/hydrogen-peroxide-produced-by-superoxide-dismutase-sod-2-is-required-for-sperm-activation-in-caenorhabditis-elegans
#10
Taro Sakamoto, Hirotaka Imai
Superoxide dismutase (SOD) is a ubiquitous antioxidant enzyme that catalytically converts the superoxide radical to hydrogen peroxide (H2O2). In mammals, high SOD activity can be detected in sperm and seminal plasma, and a correlation between loss of SOD activity and male infertility has been observed; however, the underlying mechanisms remain to be clarified. Here, we report that the deletion of two major SOD genes in Caenorhabditis elegans, sod-1 and sod-2, cause sperm activation defects, thereby leading to a significant reduction in brood size...
July 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28724621/mildew-resistance-locus-function-in-pollen-ovary-reception-is-linked-to-its-oligomerization-and-subcellular
#11
Daniel S Jones, Jing Yuan, Benjamin Smith, Andrew Willoughby, Emily L Kumimoto, Sharon A Kessler
Sexual reproduction in flowering plants requires communication between synergid cells and a tip-elongating pollen tube (PT) for the successful delivery of sperm cells to the embryo sac. The reception of the PT relies on signaling within the synergid cell that ultimately leads to the degeneration of the receptive synergid and PT rupture, releasing the sperm cells for double fertilization. In Arabidopsis thaliana, NORTIA (NTA), a member of the Mildew resistance locus O (MLO) family of proteins, plays a critical role in the communication processes regulating PT reception...
July 19, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28724182/efficacy-of-myo-inositol-in-the-clinical-management-of-patients-with-asthenozoospermia
#12
A Dinkova, D Martinov, E Konova
OBJECTIVE: The aim of this study was to analyze the effect of myo-inositol in combination with minerals and vitamin on sperm motility in patients affected by asthenozoospermia as index of Bulgarian population. PATIENTS AND METHODS: One-hundred-nine men aged between 18 and 50 years with reduced sperm motility identified by routine semen analysis were enrolled in this study. After excluding any urological problems, three months of treatment with a supplementation based on myo-inositol and other minerals and vitamins (Andrositol, MLD trading, Bulgaria) was prescribed...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28724179/contribution-of-electron-microscopy-to-study-in-vitro-inositol-effects-on-human-spermatozoa
#13
M Colone, A Calcabrini, V Unfer, A Stringaro
Infertility is a worldwide problem and male partner contributes to almost 30% of cases of infertility. The term oligoasthenoteratospermia is related to defective spermatogenesis and is characterized by a reduction of motility and number of spermatozoa and a change in their morphology. Electron microscopes are frequently used in order to evaluate sperm pathology and overall to establish a correlation between structural and functional deficiencies of altered sperm. High levels of reactive oxygen species endanger sperm function and viability...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28723592/effects-of-near-infrared-radiation-on-dna-dls-and-atr-ftir-study
#14
Katarzyna Szymborska-Małek, Małgorzata Komorowska, Marlena Gąsior-Głogowska
We presume that the primary effect of Near Infrared (NIR) radiation on aqueous solutions of biological molecules concerns modification of hydrogen bonded structures mainly the global and the hydration shell water molecules. Since water has a significant influence on the DNA structure, we expect that the thermal stability of DNA could be modified by NIR radiation. The herring sperm DNA was exposed to NIR radiation (700-1100nm) for 5, 10, and 20min periods. The temperature dependent infrared measurements were done for the thin films formed on the diamond ATR crystal from evaporated DNA solutions exposed and unexposed to NIR radiation...
July 12, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28718894/sperm-cryopreservation-and-assisted-reproductive-technology-outcome-in-patients-with-spinal-cord-injury
#15
A Reignier, J Lammers, C Splingart, D Redhead, J J Labat, S Mirallié, P Barrière, T Fréour
This study aimed to describe spinal cord injured patients' semen characteristics before and after cryopreservation, and assisted reproductive technology cycles outcome compared to the infertile population. Data about sperm analysis and assisted reproductive technology (ART) cycles outcomes for 78 men with spinal cord injury referred for sperm cryopreservation between 1998 and 2013 were retrospectively analysed and compared with a reference group consisting of every Intra Cytoplasmic Sperm Injection (ICSI) cycle performed in our in vitro fertilization unit over the 2009-2014 period...
July 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28718529/dna-fragmentation-in-spermatozoa-a-historical-review
#16
REVIEW
A S Rex, J Aagaard, J Fedder
Sperm DNA Fragmentation has been extensively studied for more than a decade. In the 1940s the uniqueness of the spermatozoa protein complex which stabilizes the DNA was discovered. In the fifties and sixties, the association between unstable chromatin structure and subfertility was investigated. In the seventies, the impact of induced DNA damage was investigated. In the 1980s the concept of sperm DNA fragmentation as related to infertility was introduced as well as the first DNA fragmentation test: the Sperm Chromatin Structure Assay (SCSA)...
July 2017: Andrology
https://www.readbyqxmd.com/read/28718528/impairment-of-sperm-dna-methylation-in-male-infertility-a-meta-analytic-study
#17
D Santi, S De Vincentis, E Magnani, G Spaggiari
Considering the widespread use of assisted reproductive techniques (ART), DNA methylation of specific genes involved in spermatogenesis achieves increasingly clinical relevance, representing a possible explanation of increased incidence of syndromes related to genomic imprinting in medically assisted pregnancies. Several trials suggested a relationship between male sub-fertility and sperm DNA methylation, although its weight on seminal parameters alteration is still a matter of debate. To evaluate whether aberrant sperm DNA methylation of imprinted genes is associated with impaired sperm parameters...
July 2017: Andrology
https://www.readbyqxmd.com/read/28712886/impacts-of-abstinence-time-on-semen-parameters-in-a-large-population-based-cohort-of-subfertile-men
#18
Sorena Keihani, James R Craig, Chong Zhang, Angela P Presson, Jeremy B Myers, William O Brant, Kenneth I Aston, Benjamin R Emery, Timothy G Jenkins, Douglas T Carrell, James M Hotaling
OBJECTIVES: To assess the effects of abstinence time on semen parameters in normozoospermic and oligozoospermic men using a large cohort of subfertile men. PATIENTS AND METHODS: From 2002-2013, we retrospectively reviewed data from 15623 patients seen at our fertility clinic. Data on patient age and semen parameters were extracted along with abstinence time. Abstinence time was categorized into 4 groups (≤2 days; >2 & ≤5 days; >5 & ≤7 days; >7 days)...
July 13, 2017: Urology
https://www.readbyqxmd.com/read/28711916/polymorphisms-of-protamine-genes-contribute-to-male-infertility-susceptibility-in-the-chinese-han-population
#19
Weijun Jiang, Peiran Zhu, Jing Zhang, Qiuyue Wu, Weiwei Li, Shuaimei Liu, Mengxia Ni, Maomao Yu, Jin Cao, Yi Li, Yingxia Cui, Xinyi Xia
Protamine (PRM) plays important roles in the packaging of DNA within the sperm nucleus. To investigate the role of PRM1/2 and transition protein 1 (TNP1) polymorphisms in male infertility, 636 infertile men and 442 healthy individuals were recruited into this case-controlled study of the Chinese Han population, using MassARRAY technology to analyze genotypes. Our analysis showed that there were no significant differences between controls and infertile cases among the five single nucleotide polymorphisms identified in PRM1, PRM2 and TNP1 [rs737008 (G/A), rs2301365 (C/A), rs2070923 (C/A), rs1646022 (C/G) and rs62180545 (A/G)]...
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28709083/the-peculiar-extra-acrosomal-structure-of-the-collembola-hexapoda-spermatozoa
#20
P P Fanciulli, C Mencarelli, D Mercati, R Dallai, P Lupetti
The springtail Collembola are characterized by having rolled spermatozoa, with a long cylindrical extracellular structure adhering to the acrosome. This structure is produced by the secretory activity of the testes epithelial cells at almost the end of spermiogenesis. At the beginning of its formation, it is a thin extension with a helical wall and a dense axial region. Later the cylindrical structure shows an inner organization which is different in the several species examined: species of Entomobryidae contain material with a paracrystalline structure, whilst some of Symphypleona contain ovoid structures...
June 29, 2017: Micron: the International Research and Review Journal for Microscopy
keyword
keyword
43384
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"