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Sperm analysis

Amena Khatun, Md Saidur Rahman, Myung-Geol Pang
The evaluation of infertility in males consists of physical examination and semen analyses. Standardized semen analyses depend on the descriptive analysis of sperm motility, morphology, and concentration, with a threshold level that must be surpassed to be considered a fertile spermatozoon. Nonetheless, these conventional parameters are not satisfactory for clinicians since 25% of infertility cases worldwide remain unexplained. Therefore, newer tests methods have been established to investigate sperm physiology and functions by monitoring characteristics such as motility, capacitation, the acrosome reaction, reactive oxygen species, sperm DNA damage, chromatin structure, zona pellucida binding, and sperm-oocyte fusion...
March 2018: Obstetrics & Gynecology Science
Simon F Schoeller, Eric E Keaveny
Swimming cells and microorganisms are as diverse in their collective dynamics as they are in their individual shapes and propulsion mechanisms. Even for sperm cells, which have a stereotyped shape consisting of a cell body connected to a flexible flagellum, a wide range of collective dynamics is observed spanning from the formation of tightly packed groups to the display of larger-scale, turbulence-like motion. Using a detailed mathematical model that resolves flagellum dynamics, we perform simulations of sperm suspensions containing up to 1000 cells and explore the connection between individual and collective dynamics...
March 2018: Journal of the Royal Society, Interface
Chetna Gangwar, Atul Saxena, Akhil Patel, S P Singh, Sarvajeet Yadav, Ravindra Kumar, Vijay Singh
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done...
March 9, 2018: Animal Reproduction Science
Mathew J Tomlinson, Asad Naeem
CASA has been used in reproductive medicine and pathology laboratories for over 25 years, yet the 'fertility industry' generally remains sceptical and has avoided automation, despite clear weaknesses in manual semen analysis. Early implementers had difficulty in validating CASA-Mot instruments against recommended manual methods (haemocytometer) due to the interference of seminal debris and non-sperm cells, which also affects the accuracy of grading motility. Both the inability to provide accurate sperm counts and a lack of consensus as to the value of sperm kinematic parameters appear to have continued to have a negative effect on CASA-Mot's reputation...
March 21, 2018: Reproduction, Fertility, and Development
Yanan Du, Yan Wang, Liqing Du, Chang Xu, Kaihua Ji, Jinhan Wang, Qiang Liu
In this data article, 146 villagers (exposed group) were randomly selected from the workers who involved in the e-wastes recycling directly as a daily job in Tianjin. Control group, including 121 villagers, came from another town without e-waste disposal sites. Chromosomal aberrations (CA) and cytokinesis blocking micronucleus (CBMN) were performed to detect the cytogenetic effect for each subject. DNA damage was detected using comet assay; the DNA percentage in the comet tail (TDNA%), tail moment (TM), and Olive tail moment (OTM) were recorded to describe DNA damage to lymphocytes and spermatozoa...
April 2018: Data in Brief
Zufferey Fanny, Rahban Rita, Garcia Arnaud, Gagnebin Yoric, Boccard Julien, Tonoli David, Jeanneret Fabienne, Stettler Eric, Senn Alfred, Nef Serge, Rudaz Serge, F Rossier Michel
Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another objective was to determine whether the presence of xenobiotics or drugs could influence these profiles. Semen analysis was performed according to WHO guidelines, and steroid profiles in blood serum and seminal plasma were determined by two complementary approaches: a targeted investigation involving the quantification of a limited number of relevant steroids for testing putative correlations with sperm parameters and a global "steroidomic" analysis highlighting their complex metabolic relationship...
March 16, 2018: Clinical Biochemistry
Osamu Kawase, Mitsuru Jimbo
Antisperm antibodies potentially inhibit sperm functions causing the sterility in humans and experimentally treated animals. However, there is no information about antisperm antibodies emerging spontaneously in wildlife. In this study, we searched for the sperm-reactive antibodies, spontaneously produced in wild sika deer (Cervus nippon), and identified the sperm antigens. We collected 529 fecal masses of sika deer in Japanese cities, from which we extracted the mucosal antibodies to test them for reactivities to deer sperm proteins by ELISA...
March 16, 2018: Journal of Veterinary Medical Science
Anita Kuriya, Chioma Agbo, Michael H Dahan
OBJECTIVE: To evaluate the relationship of one or a combination of semen analysis parameter results on insemination outcomes. MATERIAL AND METHODS: A retrospective analysis was performed to evaluate the effect on pregnancy rates in relation to one or more abnormal semen analysis parameters based on the 2010 World Health Organization semen analysis guidelines. RESULTS: Nine hundred and eighty one couples underwent 2231 intrauterine insemination cycles at the Stanford Fertility and Reproductive Medicine Center...
March 19, 2018: Journal of the Turkish German Gynecological Association
Yan-Wei Sha, Xiong Wang, Zhi-Ying Su, Chengrong Wang, Zhi-Yong Ji, Li-Bin Mei, Ling Zhang, Bing-Bing Deng, Xian-Jing Huang, Wei Yan, Jie Chen, Ping Li, Yuan-Qing Cui, Qing-Lan Qu, Chenghong Yin, Xue-Mei He
Oligoasthenoteratozoospermia (OAT) is characterized as low sperm count, decreased sperm motility and structural abnormalities of the sperm head in the same patient. However, very few studies reported the genetic alterations associated with OAT. Here we report a 38-year-old patient with OAT from a consanguineous family, with 2-6 million/mL sperm density, 2.1-3.8% normal sperm morphology and immotile sperm. Whole-exome sequencing (WES) identified homozygous variant c.1259A>G:p.Y420C in the TDRD6 gene. TDRD6 is a testis-specific expressed protein that was localized to the chromatoid bodies in germ cells and played an important role in the nonsense-mediated decay pathway...
March 15, 2018: Gene
Sh Mohammadi, M Gholamin, M Mohammadi, A Mansouri, R Mahmoodian, S Attari, S M Kebriaei, B Zibaei, M Roshanaei, F Daneshvar, M Khandehro, M A Khodadadegan, A Delshad, F Mohammadzadeh, Mostafa Peyvandi, Majid Ghayour-Mobarhan, Shima Tavallaie, Samaneh Boroumand-Noughabi, Gordon A A Ferns
In the study of the expression of CatSper genes, consideration of the effects of environmental metal toxicity is very important. Therefore, in this study, the effects of lead acetate and mercury chloride exposure on expression of CatSper genes, sperm parameters, histology of testis and prooxidant antioxidant balance (PAB) values of serum were investigated. A total of 28 mice was divided into four groups. The control group did not receive injections. The sham group received normal saline intraperitoneally. Lead and mercury groups were injected 60 and 1...
March 8, 2018: Environmental Toxicology and Pharmacology
Ross E Anderson, Heidi A Hanson, Diana Thai, Chong Zhang, Angela P Presson, Kenneth I Aston, Douglas T Carrell, Ken R Smith, James M Hotaling
PURPOSE: To study the role of individual semen parameters on the offspring birth weight and body mass index (BMI) from a population of men evaluated in an assisted reproduction technology (ART) clinic compared to fertile controls. METHODS: We performed a retrospective study using a cohort with fertile, age-matched controls of men evaluated with semen analysis at the University of Utah Andrology Clinic from 1996 to 2011 and Intermountain Healthcare from 2002 to 2011...
March 17, 2018: Journal of Assisted Reproduction and Genetics
Kris Poppe, Candice Autin, Flora Veltri, Pierre Kleynen, Lidia Grabczan, Serge Rozenberg, Lieveke Ameye
Background: Since 2010, three meta-analyses have been published on the impact of thyroid autoimmunity (TAI) on pregnancy outcomes in infertile women treated with assisted reproductive technology (ART). The initially observed high risk of miscarriage became very low in the most recent meta-analysis published in 2016. Objective: To investigate whether the lower risk of miscarriage in the latest meta-analysis was associated with the increased use of intracytoplasmic sperm injection (ICSI) in recent studies...
March 12, 2018: Journal of Clinical Endocrinology and Metabolism
Matthew R Marcello, Marina Druzhinina, Andrew Singson
The interaction and organization of proteins in the sperm membrane are important for all aspects of sperm function. We have determined the interactions between twelve known mutationally defined and cloned sperm membrane proteins in a model system for reproduction, the nematode Caenorhabditis elegans. Identification of the interactions between sperm membrane proteins will improve our understanding of and ability to characterize defects in sperm function. To identify interacting proteins, we conducted a split-ubiquitin membrane yeast two-hybrid (MYTH) analysis of gene products identified through genetic screens that are necessary for sperm function and predicted to encode transmembrane proteins...
March 13, 2018: Biology of Reproduction
Bivas Panigrahi, Chang-Hung Lu, Neha Ghayal, Chia-Yuan Chen
The zebrafish sperm activation profoundly depends upon the homogeneous mixing of the sperm cells with its diluent in a quick succession as it alters the cell's extracellular medium and initiates their motility. Manual stirring, the traditional method for zebrafish sperm activation is tedious, time-consuming, and has a poor outcome. In this aspect, an artificial cilia embedded serpentine microfluidic is designed through which hydrodynamic factors of the microfluidic environment can be precisely regulated to harness uniform mixing, hence ensuring a superior sperm activation...
March 15, 2018: Scientific Reports
Paulina A Santander Pérez, Álvaro P Ceschin, Daniela M P de Moraes, Lucileine K S N de Oliveira, Nathan I Ceschin, Nathan Ichikawa
OBJECTIVE: To analyze progesterone and prolactin plasma levels nine days after oocyte retrieval and evaluate their correlation with pregnancy rates in in vitro fertilization cycles. To achieve pregnancy, several factors are analyzed before and during the in vitro fertilization cycle. Progesterone supplementation for adequate luteal phase support is indicated despite the presence of multiple corpus luteum in IVF stimulation cycles because of blockage caused by hypothalamic agonists and antagonists...
March 15, 2018: JBRA Assisted Reproduction
Wei Zhou, Geoffry N De Iuliis, Matthew D Dun, Brett Nixon
The testicular spermatozoa of all mammalian species are considered functionally immature owing to their inability to swim in a progressive manner and engage in productive interactions with the cumulus-oocyte complex. The ability to express these key functional attributes develops progressively during the cells' descent through the epididymis, a highly specialized ductal system that forms an integral part of the male reproductive tract. The functional maturation of the spermatozoon is achieved via continuous interactions with the epididymal luminal microenvironment and remarkably, occurs in the complete absence of de novo gene transcription or protein translation...
2018: Frontiers in Endocrinology
Katarzyna Brach, Akiko Hatakeyama, Claude Nogues, Joanna Olesiak-Banska, Malcolm Buckle, Katarzyna Matczyszyn
The anisotropic shape of DNA molecules allows them to form lyotropic liquid crystals (LCs) at high concentrations. This liquid crystalline arrangement is also found in vivo (e.g., in bacteriophage capsids, bacteria or human sperm nuclei). However, the role of DNA liquid crystalline organization in living organisms still remains an open question. Here we show that in vitro, the DNA spatial structure is significantly changed in mesophases compared to non-organized DNA molecules. DNA LCs were prepared from pBluescript SK (pBSK) plasmid DNA and investigated by photochemical analysis of structural transitions (PhAST)...
March 14, 2018: Scientific Reports
Jin-Chun Lu, Jun Jing, Li Chen, Yi-Feng Ge, Rui-Xiang Feng, Yuan-Jiao Liang, Bing Yao
BACKGROUND: Many factors may lead to sperm DNA damage. However, it is little known that the correlations of sperm DNA damage with obesity-associated markers, and reproductive hormones and lipids levels in serum and seminal plasma. METHODS: In our prospective study, a total of 1 010 subfertile men, aged from 18 to 50 years old, were enrolled from August 2012 through June 2015. Their obesity-associated markers, semen parameters, sperm acrosomal enzyme activity, seminal plasma biochemical markers, and reproductive hormones and lipids levels in serum and seminal plasma were detected...
March 14, 2018: Reproductive Biology and Endocrinology: RB&E
Marja Visser, Trudie Gerrits, Fulco van der Veen, Monique Mochtar
When intended parents choose to have donor sperm treatment (DST), this may entail wide-ranging and long-lasting psychosocial implications related to the social parent not having a genetic tie with the child, how to disclose donor-conception and future donor contact. Counselling by qualified professionals is recommended to help intended parents cope with these implications. The objective of this study is to present findings and insights about how counsellors execute their counselling practices. We performed a qualitative study that included 13 counsellors working in the 11 clinics offering DST in the Netherlands...
March 14, 2018: Human Fertility: Journal of the British Fertility Society
A Mostek, M Slowinska, S Judycka, H Karol, A Ciereszko, M A Dietrich
During semen cryopreservation, spermatozoa are exposed to physical and chemical stressors that result in their functional and structural damage. Growing evidence suggests that most cryoinjuries result from oxidative stress accompanying sperm cryopreservation. Elevated amounts of reactive oxygen species (ROS) generated during cryopreservation can react with sperm macromolecules, including proteins. The goal of this study was to investigate the oxidative modifications (measured as carbonylation level changes) of carp spermatozoa proteins triggered by the cryopreservation process...
March 9, 2018: Journal of Animal Science
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