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https://www.readbyqxmd.com/read/28225103/beneficial-effects-of-lycium-barbarum-polysaccharide-on-spermatogenesis-by-improving-antioxidant-activity-and-inhibiting-apoptosis-in-streptozotocin-induced-diabetic-male-mice
#1
Guang-Jiang Shi, Jie Zheng, Jing Wu, Hai-Qi Qiao, Qing Chang, Yang Niu, Tao Sun, Yu-Xiang Li, Jian-Qiang Yu
Spermatogenic dysfunction is one of the major secondary complications of diabetes. L. barbarum polysaccharide (LBP) has long been considered to possess anti-apoptotic activities and antioxidant, anti-inflammatory and fertility-enhancing properties in traditional medical practices in China. The aim of the current study was to seek out scientific evidence to determine if LBP also contributes to the recovery from spermatogenic dysfunction in diabetic individuals. We investigated whether the oral administration of LBP in streptozotocin (STZ)-induced type-1 diabetic male mice would reverse spermatogenic dysfunction and improve histological damage in testes...
February 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28220637/effect-of-selenium-supplementation-on-semen-characteristics-of-brazil-s-ram
#2
M Piagentini, D C Silva, Cpf Dell'Aqua, C F Moya-Araujo, V M Codognoto, A A Ramos, E Oba
The aim of this study was to investigate the effects of different concentrations of oral supplementation with selenium (Se) upon ram sperm parameters. Thirty rams managed in stall under intensive system were used and divided into five groups (six animals per group) as follows: control group (G1) mineral mixture supplementation without Se, group 2 (G2) mineral mixture supplemented with 5 mg/kg Se, group 3 (G3) supplemented with 10 mg/kg Se, group 4 (G4) supplemented with 15 mg/kg Se and group 5 (G5) supplemented with 20 mg/kg Se...
February 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28220615/identification-of-sperm-morphometric-subpopulations-in-cooled-stored-canine-sperm-and-its-relation-with-sperm-dna-integrity
#3
M Urbano, I Ortiz, J Dorado, M Hidalgo
The aims of this study were to (i) identify different morphometric subpopulations in cooled-stored canine sperm and their patterns of distribution during cool-storage for up to 240 hr and (ii) determine whether or not morphometric sperm subpopulations (sP) are related to sperm DNA integrity. For that purpose, morphometric parameters were analysed by computer-assisted sperm analysis (CASA) and sperm DNA fragmentation (sDFi) using the sperm Halomax test. Four morphometric sperm heads subpopulations were identified: sP1 (large and rounded), sP2 (large and elongated), sP3 (small and rounded) and sP4 (small and elongated)...
February 20, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28220153/high-throughput-flowing-upstream-sperm-sorting-in-a-retarding-flow-field-for-human-semen-analysis
#4
Jen-Kuei Wu, Peng-Chun Chen, Yu-Nan Lin, Chia-Woei Wang, Li-Chern Pan, Fan-Gang Tseng
In this paper, we propose a microfluidic device capable of generating a retarding flow field for the sorting and separation of human motile sperm in a high-throughput manner. The proposed sorting/separation process begins with a rapid flow field in a straight-flow zone to carry sperm into a sorting zone to maintain the sperm's mobility. The sorting zone consists of a diffuser-type sperm sorter to differentiate sperm with different motilities based on the flowing upstream nature of human sperm in a retarding flow field...
February 21, 2017: Analyst
https://www.readbyqxmd.com/read/28216914/the-quality-of-sperm-preparation-medium-affects-the-motility-viability-and-dna-integrity-of-human-spermatozoa
#5
Fatemeh Anbari, Iman Halvaei, Ali Nabi, Shahin Ghazali, Mohammad Ali Khalili, Lars Johansson
AIM: The goal was to compare the effects of three different sperm preparation media on sperm motility, viability, and DNA integrity of semen samples from normozoospermic men. METHODS: A total of 15 normozoospermic males were included in the study. The semen analysis (SA) was performed in accordance with the WHO guidelines (2010). After SA, each sample was divided into three aliquots, and swim-up was performed with three different sperm preparation media (Sperm Preparation Media, Origio, Denmark; Ham's F10, Biochrome, Berlin, Germany; and VitaSperm™, Innovative Biotech, Iran)...
October 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28215690/seasonal-and-genera-specific-variations-in-semen-availability-and-semen-characteristics-in-large-parrots
#6
A Bublat, D Fischer, S Bruslund, H Schneider, S Meinecke-Tillmann, A Wehrend, M Lierz
In large parrots electro-stimulation is suitable for collecting semen, and therefore, to facilitate semen examination and artificial insemination. Previous studies have detected differences in the semen collection success rate and semen parameters between psittacine genera. It remained unclear whether these differences were genera-related, seasonal variations or depend on the males' relationship status. To answer these questions, semen collection and spermatological analysis were performed for four psittacine groups (macaws, amazons, eclectus parrots and cockatoos) over 13 months...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215685/effects-of-female-bovine-plasma-collected-at-different-days-of-the-estrous-cycle-on-epididymal-spermatozoa-motility
#7
M Nait Mouloud, F Ouennoughi, L Yaiche, R Kaidi, M Iguer-Ouada
The aim of this study was to assess the effects of female bovine plasma collected at different days of the reproductive cycle on epididymal spermatozoa motility and to test hypothesis that the subpopulations pattern of motile spermatozoa is affected by this treatment. Blood plasma samples were collected from five Holstein Friesian cows at different stages of the estrous cycle (days 0, 5, 10, 12 and 18), one pregnant cow and one adult bull and were diluted 1:9 (V/V) with normal saline. Female charcoal-treated plasma, Bull plasma and saline were used as controls...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215340/quantitative-approaches-for-the-study-of-microtubule-aster-motion-in-large-eggs
#8
H Tanimoto, N Minc
The proper positioning of microtubule (MT) asters underlies fundamental processes such as nuclear centration, cell polarity, division positioning, and embryogenesis. In large eggs and early blastomeres, MT asters may exhibit long range motions with atypical speed and precision to target their functional position. The biophysical mechanisms regulating such motions remain however largely unknown. The centration of sperm asters in sea urchin embryos is a stereotypical example of such aster long range motion. In this chapter, we describe methods developed in this system to (1) quantify sperm aster 3-D motion with confocal microscopy and automated image analysis and (2) severe a portion of astral MTs with a UV laser...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28214572/clinical-relevance-of-oxidation-reduction-potential-in-the-evaluation-of-male-infertility
#9
Ashok Agarwal, Siew May Wang
Objectives To evaluate 1) the relationship between oxidation-reduction potential (ORP) and abnormal sperm quality, 2) changes in ORP and sperm parameters over time, in search of a potential surrogate marker of poor sperm quality that may assist in the diagnosis of oxidative stress- related male infertility. Methods 194 infertile men were included and 28 patients were identified to have repeated semen analyses and ORP measurements. The semen samples obtained were categorized into normal and abnormal sperm parameters based on the World Health Organization (WHO) 5(th) edition guidelines...
February 15, 2017: Urology
https://www.readbyqxmd.com/read/28213128/quantitative-proteomic-analysis-of-human-testis-reveals-system-wide-molecular-and-cellular-pathways-associated-with-non-obstructive-azoospermia
#10
Mehdi Alikhani, Mehdi Mirzaei, Marjan Sabbaghian, Pouria Parsamatin, Razieh Karamzadeh, Samane Adib, Niloofar Sodeifi, Mohammad Ali Sadighi Gilani, Masoud Zabet-Moghaddam, Lindsay Parker, Yunqi Wo, Vivek Gupta, Paul A Haynes, Hamid Gourabi, Hossein Baharvand, Ghasem Hosseini Salekdeh
: Male infertility accounts for half of the infertility problems experienced by couples. Azoospermia, having no measurable level of sperm in seminal fluid, is one of the known conditions resulting in male infertility. In order to elucidate the complex molecular mechanisms causing male azoospermia, label-free quantitative shotgun proteomics was carried out on testicular tissue specimens from patients with obstructive azoospermia and non-obstructive azoospermia, including maturation arrest (MA) and Sertoli cell only syndrome (SCOS)...
February 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28208562/re-use-of-testicular-versus-ejaculated-sperm-for-intracytoplasmic-sperm-injection-among-men-with-cryptozoospermia-a-meta-analysis
#11
Craig Niederberger
No abstract text is available yet for this article.
March 2017: Journal of Urology
https://www.readbyqxmd.com/read/28207000/spatial-transcriptomic-analysis-of-cryosectioned-tissue-samples-with-geo-seq
#12
Jun Chen, Shengbao Suo, Patrick Pl Tam, Jing-Dong J Han, Guangdun Peng, Naihe Jing
Conventional gene expression studies analyze multiple cells simultaneously or single cells, for which the exact in vivo or in situ position is unknown. Although cellular heterogeneity can be discerned when analyzing single cells, any spatially defined attributes that underpin the heterogeneous nature of the cells cannot be identified. Here, we describe how to use Geo-seq, a method that combines laser capture microdissection (LCM) and single-cell RNA-seq technology. The combination of these two methods enables the elucidation of cellular heterogeneity and spatial variance simultaneously...
March 2017: Nature Protocols
https://www.readbyqxmd.com/read/28203244/pistil-transcriptome-analysis-to-disclose-genes-and-gene-products-related-to-aposporous-apomixis-in-hypericum-perforatum-l
#13
Giulio Galla, Sara Zenoni, Linda Avesani, Lothar Altschmied, Paride Rizzo, Timothy F Sharbel, Gianni Barcaccia
Unlike sexual reproduction, apomixis encompasses a number of reproductive strategies, which permit maternal genome inheritance without genetic recombination and syngamy. The key biological features of apomixis are the circumvention of meiosis (i.e., apomeiosis), the differentiation of unreduced embryo sacs and egg cells, and their autonomous development in functional embryos through parthenogenesis, and the formation of viable endosperm either via fertilization-independent means or following fertilization with a sperm cell...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28194272/fatty-acid-content-in-epididymal-fluid-and-spermatozoa-during-sperm-maturation-in-dogs
#14
Daniel S Ramos Angrimani, Marcilio Nichi, João Diego A Losano, Cristina F Lucio, Gisele A Lima Veiga, Márcia V M Junqueira Franco, Camila I Vannucchi
BACKGROUND: During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28191666/appraisal-and-standardization-of-curvilinear-velocity-vcl-cut-off-values-for-casa-analysis-of-japanese-quail-coturnix-japonica-sperm
#15
U Farooq, I A Malecki, M Mahmood, G B Martin
One of the basic steps in objective analysis of sperm motility is the subdivision of a motile sperm population into slow, medium and rapid categories based on their velocity. However, for CASA analysis of quail sperm, the velocity values for categorization of slow, medium and rapid sperm have not yet been standardized. To identify the cut-off values of "velocity curvilinear" (VCL) for quail sperm categorization, we captured and analysed 22,300 tracks of quail sperm using SCA(®) -CASA. The median and mean VCL values were 85 and 97 μm/s...
February 13, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28191261/global-dna-methylation-levels-are-altered-by-modifiable-clinical-manipulations-in-assisted-reproductive-technologies
#16
Jayashri Ghosh, Christos Coutifaris, Carmen Sapienza, Monica Mainigi
BACKGROUND: We analyzed placental DNA methylation levels at repeated sequences (LINE1 elements) and all CCGG sites (the LUMA assay) to study the effect of modifiable clinical or laboratory procedures involved in in vitro fertilization. We included four potential modifiable factors: oxygen tension during embryo culture, fresh embryo transfer vs frozen embryo transfer, intracytoplasmic sperm injection (ICSI) vs conventional insemination or day 3 embryo transfer vs day 5 embryo transfer...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28187743/the-influence-of-macro-and-microelements-in-seminal-plasma-on-diluted-boar-sperm-quality
#17
Maja Zakošek Pipan, Janko Mrkun, Breda Jakovac Strajn, Katarina Pavšič Vrtač, Janko Kos, Anja Pišlar, Petra Zrimšek
BACKGROUND: Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15-17 °C...
February 10, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28187507/diagnostic-genetic-screening-for-assisted-reproductive-technologies-patients-with-macrozoospermia
#18
V Carmignac, J-M Dupont, R C Fierro, J Barberet, C Bruno, N Lieury, E Dulioust, J Auger, P Fauque
Macrozoospermia is characterized by a high proportion of abnormal spermatozoa with enlarged heads. So far, it has been associated with mutations only in the Aurora Kinase C gene (AURKC) in some cases. Although many publications have reported failure to conceive in couples with macrozoospermia, a few others have described successful pregnancies, thus raising questions as to whether ICSI and AURKC genetic screening should be recommended in all patients with macrozoospermia. First, we report on two monozygotic twins presenting macrozoospermia for whom the genetic status was explored (Aurora Kinase C sequencing) and whole semen and gradient-selected spermatozoa were analyzed, using Fluorescent In Situ Hybridization (FISH), Electron Microscopy and flow cytometry...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28185602/impaired-hypothalamic-pituitary-testicular-axis-activity-spermatogenesis-and-sperm-function-promote-infertility-in-males-with-lead-poisoning
#19
Jason Gandhi, Rafael J Hernandez, Andrew Chen, Noel L Smith, Yefim R Sheynkin, Gargi Joshi, Sardar Ali Khan
Lead poisoning is a stealthy threat to human physiological systems as chronic exposure can remain asymptomatic for long periods of time before symptoms manifest. We presently review the biophysical mechanisms of lead poisoning that contribute to male infertility. Environmental and occupational exposure of lead may adversely affect the hypothalamic-pituitary-testicular axis, impairing the induction of spermatogenesis. Dysfunction at the reproductive axis, namely testosterone suppression, is most susceptible and irreversible during pubertal development...
February 10, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28185121/the-association-between-coenzyme-q10-concentrations-in-follicular-fluid-with-embryo-morphokinetics-and-pregnancy-rate-in-assisted-reproductive-techniques
#20
Süleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Zeliha Günnur Dikmen, Mustafa Agah Tekindağ
PURPOSE: This study seeks to evaluate the association between follicular fluid (FF) coenzyme Q10 (CoQ10) levels, embryo morphokinetics, and pregnancy rate. METHODS: Sixty infertile patients who underwent intracytoplasmic sperm injection (ICSI) cycles were included in the study. For each patient, CoQ10 level of the follicular fluid was measured by high-performance liquid chromatography system. After the ICSI of each oocyte, the relationship between the level of CoQ10 content of each follicular fluid, the subsequent embryo quality, and embryo morphokinetics was investigated...
February 9, 2017: Journal of Assisted Reproduction and Genetics
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