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https://www.readbyqxmd.com/read/29781510/tramadol-opioid-abuse-is-associated-with-a-dose-and-time-dependent-poor-sperm-quality-and-hyperprolactinaemia-in-young-men
#1
A G A Farag, M A Basha, S A Amin, N F Elnaidany, N G Elhelbawy, M M T Mostafa, S A Khodier, R A Ibrahem, R Z Mahfouz
Tramadol, one of the most commonly abused drugs in Middle East, impacts spermatogenesis and disturbs reproductive hormones in animal studies. We aimed to investigate tramadol impact on sperm quality and on levels of testosterone, prolactin and gonadotropins, in tramadol abusers (n = 30) to age-matched control (n = 30). Abusers had significantly low percentages of sperm motility, normal forms and vitality compared with control (95% CI -40.7 to -19.3, -13.5 to -9.3 and -31.9 to -9.7 respectively). Hypoandrogenism (95% CI -4...
May 21, 2018: Andrologia
https://www.readbyqxmd.com/read/29780028/induced-endometrial-trauma-endometrial-scratch-in-the-mid-luteal-menstrual-cycle-phase-preceding-first-cycle-ivf-icsi-versus-usual-ivf-icsi-therapy-study-protocol-for-a-randomised-controlled-trial
#2
Clare Pye, Robin Chatters, Judith Cohen, Kate Brian, Ying C Cheong, Susan Laird, Lamiya Mohiyiddeen, Jonathan Skull, Stephen Walters, Tracey Young, Mostafa Metwally
INTRODUCTION: Endometrial trauma commonly known as endometrial scratch (ES) has been shown to improve pregnancy rates in women with a history of repeated implantation failure undergoing in vitro fertilisation (IVF), with or without intracytoplasmic sperm injection (ICSI). However, the procedure has not yet been fully explored in women having IVF/ICSI for the first time. This study aims to examine the effect of performing an ES in the mid-luteal phase prior to a first-time IVF/ICSI cycle on the chances of achieving a clinical pregnancy and live birth...
May 20, 2018: BMJ Open
https://www.readbyqxmd.com/read/29779447/development-of-postmeiotic-cells-in-vitro-from-spermatogonial-cells-of-prepubertal-cancer-patients
#3
Maram Abofoul-Azab, Ali AbuMadigem, Eitan Lunenfeld, Joseph Kapelushnik, Qinghua Shi, Haim Pinkas, Mahmoud Huleihel
Aggressive chemotherapy in childhood often results in testicular damage and consequently jeopardizes future fertility. The presence of spermatogonial cells (SPGCs) in the testes of prepubertal cancer patient boys (PCPBs) can be used to develop future strategies for male fertility preservation. In the present study, we examined the presence of SPGCs in testes of chemotherapy-treated PCPBs and their ability to develop spermatogenesis in vitro using a three-dimensional (3D) culture system. Seven testicular biopsies were obtained from chemotherapy-treated PCPBs and one from a patient with β-thalassemia major...
May 19, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#4
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29778923/quantitative-proteomic-profiling-indicates-the-difference-in-reproductive-efficiency-between-meishan-and-duroc-boar-spermatozoa
#5
Li Xinhong, Linqing Zhen, Jieli Fu, Lirui Wang, Qiangzhen Yang, Peifei Li, Yuhua Li
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported. Therefore, we applied iTRAQ labeling coupled with mass spectrometry, and analyzed the differences in proteins between Meishan and Duroc sperm. In the present study, a total of 1597 proteins were quantified. Of these proteins, 190 showed statistically significant fold changes between Meishan and Duroc spermatozoa...
April 30, 2018: Theriogenology
https://www.readbyqxmd.com/read/29778554/cumulative-live-birth-rates-after-ivf-in-patients-with-polycystic-ovaries-phenotype-matters
#6
Michel De Vos, Stéphanie Pareyn, Panagiotis Drakopoulos, José M Raimundo, Ellen Anckaert, Samuel Santos-Ribeiro, Nikolaos P Polyzos, Herman Tournaye, Christophe Blockeel
RESEARCH QUESTION: Do cumulative live birth rates (CLBR) vary among women with different polycystic ovary syndrome (PCOS) phenotypes who undergo IVF/intracytoplasmic sperm injection (ICSI) treatment? DESIGN: In this retrospective cohort study, data from 567 patients undergoing an assisted reproductive technology (ART) cycle between January 2010 and December 2015 were collected. Demographical traits, cycle characteristics and clinical and laboratory data were analysed...
May 7, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29778381/dietary-patterns-are-positively-associated-with-semen-quality
#7
Michal Efrat, Anat Stein, Haim Pinkas, Ron Unger, Ruth Birk
OBJECTIVE: To study association of semen quality with a priori whole dietary pattern indexes, which reflect real-world dietary practices and the numerous combinations by which foods are consumed: Healthy Eating Index (HEI), Dietary Approaches to Stop Hypertension (DASH), alternate Mediterranean Diet score (aMED), and Alternative Healthy Eating Index (AHEI). DESIGN: A cross-sectional single-center study. SETTING: Hospital fertility center and university...
May 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29777550/evidence-for-dietary-time-series-in-layers-of-cetacean-skin-using-stable-carbon-and-nitrogen-isotope-ratios
#8
Lauren A Wild, Ellen M Chenoweth, Franz J Mueter, Janice M Straley
RATIONALE: Stable isotope analysis integrates diet information over a time period specific to the type of tissue sampled. For metabolically active skin of free-ranging cetaceans, cells are generated at the basal layer of the skin and migrate outward until they eventually slough off, suggesting potential for a dietary time series. METHODS: Skin samples from cetaceans were analyzed using continuous-flow elemental analyzer isotope ratio mass spectrometery (EA-IRMS)...
May 18, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29777417/effects-of-dietary-microencapsulated-cymbopogon-flexuosus-essential-oil-on-reproductive-related-parameters-in-male-rhamdia-quelen
#9
Carine de Freitas Souza, Cristine Rampelotto, Bruno Bianchi Loureiro, Fernanda Alves Pereira, Adriane Erbice Bianchini, Carine Dahl Corcini, Antonio Sergio Varela Junior, Tatiana Emanuelli, Leila Picolli da Silva, Sílvio Teixeira da Costa, Kalyne Bertolin, Monique Tomazele Rovani, Paulo Bayard Dias Gonçalves, Berta Maria Heinzmann, Bernardo Baldisserotto
In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish (Rhamdia quelen) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0 = control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl, smtl, pomca, and pomcb was assessed in the pituitary gland by real-time PCR...
May 18, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29777145/testicular-sperm-is-superior-to-ejaculated-sperm-for-icsi-in-cryptozoospermia-an-update-systematic-review-and-meta-analysis
#10
Yi-No Kang, Ya-Wen Hsiao, Chien-Yu Chen, Chien-Chih Wu
Intracytoplasmic sperm injection (ICSI) is well established and provides patients with severely impaired sperm quality with an opportunity to father a child. However, previous studies do not clearly indicate whether male with cryptozoospermia should use testicular sperm or ejaculated sperm for ICSI. The newest systematic review of this topic also gave a controversial conclusion that was based on incorrect pooling result. Moreover, two clinical studies published after the systematic review. In the present update systematic review and meta-analysis, a comprehensive citation search for relevant studies was performed using the Cochrane library databases, Embase, Ovid MEDLINE, PubMed, ScienceDirect, Scopus, and Web of Science up to September 2017...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29774482/the-effect-of-subclinical-varicocele-on-pregnancy-rates-and-semen-parameters-a-systematic-review-and-meta-analysis
#11
REVIEW
Taylor P Kohn, Samuel J Ohlander, Jake S Jacob, Tina M Griffin, Larry I Lipshultz, Alexander W Pastuszak
PURPOSE OF REVIEW: Current guidelines recommend against surgical repair of subclinical varicoceles (SCVs) for infertility; several studies demonstrate mixed fertility results after SCV correction. To determine whether surgical correction of SCV improves semen parameters and/or reproductive outcomes, we performed a systematic review and meta-analysis. Seven biomedical literature databases were searched through January 2018 for studies that assessed reproductive outcomes and/or change in semen parameters in men with corrected SCV compared to either (1) uncorrected SCV or (2) corrected clinical varicocele...
May 17, 2018: Current Urology Reports
https://www.readbyqxmd.com/read/29774143/expression-of-trpc5-is-decreased-in-the-sperm-of-patients-with-varicocele-associated-asthenozoospermia
#12
Guangbin Zhu, Changying Xie, Zhonghua Yang, Yongzhi Wang, Dong Chen, Xinghuan Wang
The present study aimed to determine whether the expression of transient receptor potential channel 5 (TRPC5) protein is altered in spermatozoa of patients with varicocele-associated asthenozoospermia. TRPC5 expression in spermatozoa was determined by polymerase chain reaction and western blotting analyses, and indirect immunofluorescence was used for identification and immunolocalization of the TRPC5 channel in human sperm. Sperm motility and superoxide dismutase (SOD) activity were also determined with a computer-assisted semen analysis system and assay kit, respectively...
June 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29774101/qilin-pills-alleviate-oligoasthenospermia-by-inhibiting-bax-caspase-9-apoptosis-pathway-in-the-testes-of-model-rats
#13
Kaishu Zhang, Zhengyan Ge, Longlong Fu, Qi An, Fang Zhou, Ying Guo, Xiaowei Wang, Wenhong Lu, Xiaowei Liang, Shusong Wang, Xuejun Shang, Yiqun Gu
At present, the treatment of oligoasthenospermia with western medicine is ineffective. Qilin pill (QLP) is a Chinese traditional medicine for treating male infertility. Recent multicenter clinical studies in China reported that QLPs markedly improved sperm quality. However, the mechanism of action of QLPs on oligoasthenospermia remains unknown. In this study, we investigated the mechanistic basis for improvement of semen parameters and reversal of testis damage by QLPs in a rat model of oligoasthenospermia induced by treatment with tripterygium glycosides (TGs) (40 mg/kg) once daily for 4 weeks...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29769570/in-utero-and-postnatal-exposure-to-high-fat-high-sucrose-diet-suppressed-testis-apoptosis-and-reduced-sperm-count
#14
Jiude Mao, Kathleen A Pennington, Omonseigho O Talton, Laura C Schulz, Miriam Sutovsky, Yan Lin, Peter Sutovsky
Obesity affects male fertility and maternal diabetes affects the offspring sperm epigenome. However, the effects of in utero exposure to maternal glucose intolerance in combination with postnatal high fat, high sucrose (HFHS) diet consumption on offspring spermatogenesis is not clear. The present study was designed to test these effects. One week before and during pregnancy, dams were fed either control or HFHS diet to induce gestational glucose intolerance, and returned to standard diet during lactation. Male offspring from each maternal group were split into control and HFHS-fed groups for eight weeks prior to sacrifice at 11, 19 or 31 weeks of age, and reproductive tissues were harvested for analysis of testicular germ cell apoptosis and sperm output...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769389/extracts-for-analysis-of-dna-replication-in-a-nucleus-free-system
#15
Justin Sparks, Johannes C Walter
Frog egg extracts represent a powerful approach with which to dissect molecular mechanisms of vertebrate DNA replication and repair. In the classical approach, sperm chromatin is added to a crude egg lysate to form replication-competent nuclei. We subsequently described a procedure that bypasses the requirement for nuclear assembly in DNA replication. In this method, DNA is first added to a high-speed supernatant (HSS) of egg lysate, which mimics the G1 phase of the cell cycle in that it supports replication licensing...
May 16, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29769123/obesity-impairs-male-fertility-through-long-term-effects-on-spermatogenesis
#16
Yan-Fei Jia, Qian Feng, Zheng-Yan Ge, Ying Guo, Fang Zhou, Kai-Shu Zhang, Xiao-Wei Wang, Wen-Hong Lu, Xiao-Wei Liang, Yi-Qun Gu
OBJECTIVE: This study aimed to investigate the effect and possible underlying mechanisms of high-fat diet-induced obesity on spermatogenesis in male rats. METHODS: A total of 45 male rats were randomly divided into control (n = 15, normal diet) and obesity groups (n = 30, high-fat diet) and were fed for 16 weeks. Body weight and organ indexes were determined after sacrifice. Indicators of reproductive function, including sperm count, sperm motility, apoptosis of spermatogenic cells, and oxidative stress levels, were measured...
May 16, 2018: BMC Urology
https://www.readbyqxmd.com/read/29768446/inhibitory-effect-of-k-ions-and-influence-of-other-ions-and-osmolality-on-the-spermatozoa-motility-of-european-burbot-lota-lota-l
#17
Katarzyna Dziewulska, Malwina Pilarska
BACKGROUND: In fish with external fertilization, two main start-up mechanisms of the path that blocks or activates the spermatozoan motility apparatus are known. The main factor managing the path is osmolality or potassium ion. In burbot from the European and North American population, contradictory findings regarding the factors influencing the onset of spermatozoa motility were reported. The objective of the current study was to determine the effect of potassium and osmolality on the spermatozoa activation of European burbot, Lota lota (Actinopterygii, Gadiformes, Lotidae)...
2018: PloS One
https://www.readbyqxmd.com/read/29763586/postnatal-testis-development-in-the-collared-peccary-tayassu-tajacu-with-emphasis-on-spermatogonial-stem-cells-markers-and-niche
#18
Nathália de Lima E Martins Lara, Guilherme Mattos Jardim Costa, Gleide Fernandes de Avelar, Diva Anelie Guimarães, Luiz Renato de França
Collared peccaries (Tayassu tajacu) present a unique testis cytoarchitecture, where Leydig cells (LC) are mainly located in cords around the seminiferous tubules (ST) lobes. This peculiar arrangement is very useful to better investigate and understand the role of LC in spermatogonial stem cells (SSCs) biology and niche. Recent studies from our laboratory using adult peccaries have shown that the undifferentiated type A spermatogonia (Aund or SSCs) are preferentially located in ST regions adjacent to the intertubular compartment without LC...
May 12, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29761541/telfairia-occidentalis-supplemented-diet-induces-changes-in-sperm-parameters-and-testosterone-level-in-rats
#19
R-C C Njoku, S O Abarikwu, A A Uwakwe, C J Mgbudom-Okah
The growing patronage on herbal remedies and formulations from natural products in most developing countries has warranted research into certain health challenges including their antifertility effects. This study assessed the effects of boiled Telfairia occidentalis (TO) seed-supplemented diets on the level of testosterone and semen quality in Wistar rats. Boiled TO seed diets at 10%, 15% and 30% were given to rats for 60 days. Our study showed that sperm quality was impaired as evidenced by the decreased number of motile spermatozoa, epididymal sperm numbers, percentage live/dead ratio and increased numbers of abnormal spermatozoa comparable to control values (p < ...
May 14, 2018: Andrologia
https://www.readbyqxmd.com/read/29760927/is-sperm-fish-analysis-still-useful-for-robertsonian-translocations-meiotic-analysis-for-23-patients-and-review-of-the-literature
#20
Anna Lamotte, Guillaume Martinez, Françoise Devillard, Jean-Pascal Hograindleur, Véronique Satre, Charles Coutton, Radu Harbuz, Florence Amblard, James Lespinasse, Mehdi Benchaib, Julien Bessonnat, Sophie Brouillet, Sylviane Hennebicq
Background: Robertsonian translocations (RobT) are common structural chromosome rearrangements where carriers display a majority of chromosomally balanced spermatozoa from alternate segregation mode. According to some monotony observed in the rates of balanced segregation, is sperm FISH analysis obsolete for RobT carriers? Methods: Retrospective cohort research study on 23 patients analyzed in our center from 2003 to 2017 and compared to the data of 187 patients in literature from 1983 to 2017...
2018: Basic and Clinical Andrology
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