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https://www.readbyqxmd.com/read/28535307/intergenerational-impact-of-paternal-lifetime-exposures-to-both-folic-acid-deficiency-and-supplementation-on-reproductive-outcomes-and-imprinted-gene-methylation
#1
Lundi Ly, Donovan Chan, Mahmoud Aarabi, Mylène Landry, Nathalie A Behan, Amanda J MacFarlane, Jacquetta Trasler
STUDY QUESTION: Do paternal exposures to folic acid deficient (FD), and/or folic acid supplemented (FS) diets, throughout germ cell development adversely affect male germ cells and consequently offspring health outcomes? SUMMARY ANSWER: Male mice exposed over their lifetimes to both FD and FS diets showed decreased sperm counts and altered imprinted gene methylation with evidence of transmission of adverse effects to the offspring, including increased postnatal-preweaning mortality and variability in imprinted gene methylation...
May 23, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28534180/the-c-terminal-kinesin-motor-kifc1-may-participate-in-nuclear-reshaping-and-flagellum-formation-during-spermiogenesis-of-larimichthys-crocea
#2
Dan-Dan Zhang, Xin-Ming Gao, Yong-Qiang Zhao, Cong-Cong Hou, Jun-Quan Zhu
Spermatogenesis is a highly ordered process in the differentiation of male germ cells. Nuclear morphogenesis is one of the most fundamental cellular transformations to take place during spermatogenesis. These striking transformations from spermatogonia to spermatozoa are a result of phase-specific adaption of the cytoskeleton and its association with molecular motor proteins. KIFC1 is a C-terminal kinesin motor protein that plays an essential role in acrosome formation and nuclear reshaping during spermiogenesis in mammals...
May 23, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28533967/divergent-evolutionary-histories-of-dna-markers-in-a-hawaiian-population-of-the-coral-montipora-capitata
#3
Hollie M Putnam, Diane K Adams, Ehud Zelzion, Nicole E Wagner, Huan Qiu, Tali Mass, Paul G Falkowski, Ruth D Gates, Debashish Bhattacharya
We investigated intra- and inter-colony sequence variation in a population of the dominant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies...
2017: PeerJ
https://www.readbyqxmd.com/read/28531319/improved-sperm-kinematics-in-semen-samples-collected-after-2-h-versus-4-7-days-of-ejaculation-abstinence
#4
H Alipour, G Van Der Horst, O B Christiansen, F Dardmeh, N Jørgensen, H I Nielsen, C Hnida
STUDY QUESTION: Does a short abstinence period of only 2 h yield spermatozoa with better motility characteristics than samples collected after 4-7 days? SUMMARY ANSWER: Despite lower semen volume, sperm concentration, total sperm counts and total motile counts, higher percentages of motile spermatozoa with higher velocity and progressiveness were detected in samples obtained after 2 h. WHAT IS KNOWN ALREADY: Most studies that have assessed the effect of abstinence periods on sperm motility parameters in men with a sperm concentration below 15 million/ml have detected a higher percentage of motile spermatozoa in samples obtained after short abstinence periods...
May 22, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28525330/effect-of-boar-ejaculate-fraction-extender-type-and-time-of-storage-on-quality-of-spermatozoa
#5
A Dziekońska, K Świąder, M Koziorowska-Gilun, K Mietelska, Ł Zasiadczyk, W Kordan
The aim of this study was to investigate the effect the sperm-rich fraction (F1) and the post-F1 fraction (F2) on the quality of boar spermatozoa stored in a liquid state. Ejaculates were collected from three Polish Landrace boars. Each ejaculate fraction was diluted with BTS short-term extender and Safe-Cell Plus (SCP) long-term extender and stored for seven days (D1-D7) at 17°C. Analyses included sperm motility parameters, normal apical ridge (NAR) acrosomes and plasma membrane integrity (PMI). Prior to the dilution of fractions, marked changes (p<0...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28525327/in-vitro-fertilizing-potential-of-urethral-and-epididymal-spermatozoa-collected-from-domestic-cats-felis-catus
#6
S Prochowska, W Niżański
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28522219/racial-variation-in-semen-quality-at-fertility-evaluation
#7
Yash S Khandwala, Chiyuan A Zhang, Shufeng Li, Barry Behr, David Guo, Michael L Eisenberg
OBJECTIVE: To identify racial differences in semen quality among men living in the same geographic area seeking fertility evaluation. METHODS: Men obtaining a semen analysis for infertility evaluation or treatment between 2012 and 2016 at a single center were identified and demographic data including height, weight, BMI and age were described. Mean semen parameters and the proportions of men with suboptimal parameters based on the WHO 5(th) edition criteria were also compared based on race...
May 15, 2017: Urology
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#8
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP-induced sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
May 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#9
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28520588/evaluation-of-the-quality-of-semen-and-sexual-function-in-men-with-inflammatory-bowel-disease
#10
Paz Valer, Alicia Algaba, Daniel Santos, Manuel E Fuentes, Elva Nieto, Javier P Gisbert, Pilar López, Elvira Quintanilla, Francisco J García-Alonso, Iván Guerra, Álvaro Páez, Fernando Bermejo
BACKGROUND: Data on fertility rates and medication safety in men with inflammatory bowel disease (IBD) are limited. The aim of this study was to evaluate whether there is a seminal alteration in patients with IBD and, if so, to evaluate the mechanisms that may play a role according to what has been described in the literature. Its secondary aim was to evaluate the impact on male sexual function of IBD. METHODS: Multicenter, cross-sectional, case series study comparing men with IBD and control subjects...
May 16, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28512759/effect-of-ph-on-rheotaxis-of-bull-sperm-using-microfluidics
#11
T M El-Sherry, M A Abdel-Ghani, N S Abou-Khalil, M Elsayed, M Abdelgawad
The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (1:10). pH of the medium was adjusted using a digital pH meter to the following pH values: 6.0, 6.2, 6.4, 6.4, 6.8, 7.0. All kinetic parameters of sperm (n = 3,385) were determined through a computer-assisted sperm analysis (CASA) system using microfluidic devices with controlled flow velocity. The following parameters were determined: total motility (TM%), positive rheotaxis (PR%), straightline velocity (VSL, μm/s), average path velocity (VAP, μm/s), linearity (LIN, as VSL/VCL, %), beat cross-frequency (BCF, Hz) and curvilinear velocity (VCL, μm/s)...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28511862/effect-of-different-concentrations-of-egg-yolk-and-virgin-coconut-oil-in-tris-based-extenders-on-chilled-and-frozen-thawed-bull-semen
#12
A A Tarig, H Wahid, Y Rosnina, N Yimer, Y M Goh, F H Baiee, A M Khumran, H Salman, M Ebrahimi
The aim of this study was to evaluate the effects of 8% virgin coconut oil (VCO) combined with different percentages of egg yolk in Tris extender on the quality of chilled and frozen-thawed bull semen. A total of 24 ejaculates from four bulls were collected using an electroejaculator. Semen samples were diluted with 8% VCO in Tris extender which contained different concentrations 0% (control), 4%, 8%, 12%, 16% and 20% egg yolk. The diluted semen samples were divided into two fractions: one was chilled and stored at 4°C until evaluation after 24, 72, and 144h; the second fraction was processed by chilling for 3h at 4°C to equilibrate, then packaged in 0...
April 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28511094/isolation-and-genetic-analysis-of-pure-cells-from-forensic-biological-mixtures-the-precision-of-a-digital-approach
#13
F Fontana, C Rapone, G Bregola, R Aversa, A de Meo, G Signorini, M Sergio, A Ferrarini, R Lanzellotto, G Medoro, G Giorgini, N Manaresi, A Berti
Latest genotyping technologies allow to achieve a reliable genetic profile for the offender identification even from extremely minute biological evidence. The ultimate challenge occurs when genetic profiles need to be retrieved from a mixture, which is composed of biological material from two or more individuals. In this case, DNA profiling will often result in a complex genetic profile, which is then subject matter for statistical analysis. In principle, when more individuals contribute to a mixture with different biological fluids, their single genetic profiles can be obtained by separating the distinct cell types (e...
May 1, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28510703/long-chain-fatty-acid-triglyceride-tg-metabolism-disorder-impairs-male-fertility-a-study-using-adipose-triglyceride-lipase-deficient-mice
#14
Hidetake Masaki, Namhyo Kim, Hitomi Nakamura, Keiichi Kumasawa, Eriko Kamata, Ken-Ichi Hirano, Tadashi Kimura
STUDY QUESTION: Does the deletion of adipose triglyceride lipase (Atgl) gene impair male fertility? SUMMARY ANSWER: The deletion of Atgl gene impaired male fertility but the effect was partially reversed by a low long chain triglyceride (TG) diet. WHAT IS KNOWN ALREADY: ATGL specifically hydrolyses long-chain fatty acid triglyceride (TG) to diacylglycerol and a high level of expression of ATGL in testes has been reported. However, the role of ATGL in male fertility is unknown...
May 16, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28510469/outer-dense-fiber-proteins-bridging-between-male-infertility-and-cancer
#15
Faezeh Azizi, Soudeh Ghafouri-Fard
BACKGROUND: The similarities between gametogenic and carcinogenesis processes have been noted for more than decades. Among prominent similarities between these two processes is expression of a group of antigens, namely cancer-testis antigens in both the testes and various cancer tissues. Outer dense fiber (ODF) proteins are testis-specific proteins localized to sperm tails and involved in sperm motility. METHODS: We performed a computerized search of the MEDLINE/PUBMED databases with keywords "outer dense fiber, ODF, cancer, testis, gametogenesis and infertility"...
May 2017: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/28501977/characterization-of-mitochondrial-prohibitin-from-boleophthalmus-pectinirostris-and-evaluation-of-its-possible-role-in-spermatogenesis
#16
Di Wang, Yong-Qiang Zhao, Ying-Li Han, Cong-Cong Hou, Jun-Quan Zhu
Prohibitin (PHB) is an evolutionarily conserved mitochondrial membrane protein. It plays a vital role in cell proteolysis, senescence, and apoptosis and is associated with spermatogenesis and sperm quality control in mammals. To study the characteristics of the PHB gene and its potential roles during spermatogenesis in Boleophthalmus pectinirostris, we cloned a 1153-bp full-length cDNA from the testis of B. pectinirostris with an open reading frame of 816 bp, which encodes 272 amino acid residues. Real-time quantitative PCR (qPCR) analysis revealed the presence of phb mRNA in all the tissues examined, with higher expression levels found in the testis, kidney, intestine, and muscle tissues...
May 13, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28500397/analysis-of-lethal-and-sublethal-impacts-of-environmental-disasters-on-sperm-whales-using-stochastic-modeling
#17
Azmy S Ackleh, Ross A Chiquet, Baoling Ma, Tingting Tang, Hal Caswell, Amy Veprauskas, Natalia Sidorovskaia
Mathematical models are essential for combining data from multiple sources to quantify population endpoints. This is especially true for species, such as marine mammals, for which data on vital rates are difficult to obtain. Since the effects of an environmental disaster are not fixed, we develop time-varying (nonautonomous) matrix population models that account for the eventual recovery of the environment to the pre-disaster state. We use these models to investigate how lethal and sublethal impacts (in the form of reductions in the survival and fecundity, respectively) affect the population's recovery process...
May 12, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28499169/two-novel-dinuclear-ellipsoid-ni-ii-and-co-ii-complexes-bridged-by-4-5-bis-pyrazol-1-yl-phthalic-acid-synthesis-structural-characterization-and-biological-evaluation
#18
Enjun Gao, Zhenzhen Qi, Yun Qu, Yuqing Ding, Yang Zhan, Na Sun, Shaozhong Zhang, Xue Qiu, Mingchang Zhu
Two novel complexes, [Ni2(L)2(H2O)3]·4(H2O) (1) and [Co2(L)2(H2O)3]·5(H2O) (2) [H2L = 4,5-bis(pyrazol-1-yl) phthalic acid] were synthesized and characterized by spectroscopy (IR, (1)H NMR), and elemental analysis. The structures for the complexes were determined by X-ray crystallography providing the dinuclear ellipsoid Ni(II) and Co(II) complexes bridged by 4,5-bis(pyrazol-1-yl)phthalic acid ligands with same coordination modes. The interaction capacity of the complexes with FS-DNA (fish sperm DNA) has been investigated by UV and fluorescence spectroscopy...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28498920/copy-number-variation-of-functional-rbmy1-is-associated-with-sperm-motility-an-azoospermia-factor-linked-candidate-for-asthenozoospermia
#19
Yuanlong Yan, Xiling Yang, Yunqiang Liu, Ying Shen, Wenling Tu, Qiang Dong, Dong Yang, Yongyi Ma, Yuan Yang
STUDY QUESTION: What is the influence of copy number variation (CNV) in functional RNA binding motif protein Y-linked family 1 (RBMY1) on spermatogenic phenotypes? SUMMARY ANSWER: The RBMY1 functional copy dosage is positively correlated with sperm motility, and dosage insufficiency is an independent risk factor for asthenozoospermia. WHAT IS KNOWN ALREADY: RBMY1, a multi-copy gene expressed exclusively in the adult testis, is one of the most important candidates for male infertility in the azoospermia factor (AZF) region of the Y-chromosome...
May 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28497410/could-monopronucleated-icsi-zygotes-be-considered-for-transfer-analysis-through-time-lapse-monitoring-and-pgs
#20
S Mateo, F Vidal, M Parriego, I Rodríguez, V Montalvo, A Veiga, M Boada
PURPOSE: The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage. METHODS: Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator...
May 11, 2017: Journal of Assisted Reproduction and Genetics
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