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https://www.readbyqxmd.com/read/27918557/automated-evaluation-of-liver-fibrosis-in-thioacetamide-carbon-tetrachloride-and-bile-duct-ligation-rodent-models-using-second-harmonic-generation-two-photon-excited-fluorescence-microscopy
#1
Feng Liu, Long Chen, Hui-Ying Rao, Xiao Teng, Ya-Yun Ren, Yan-Qiang Lu, Wei Zhang, Nan Wu, Fang-Fang Liu, Lai Wei
Animal models provide a useful platform for developing and testing new drugs to treat liver fibrosis. Accordingly, we developed a novel automated system to evaluate liver fibrosis in rodent models. This system uses second-harmonic generation (SHG)/two-photon excited fluorescence (TPEF) microscopy to assess a total of four mouse and rat models, using chemical treatment with either thioacetamide (TAA) or carbon tetrachloride (CCl4), and a surgical method, bile duct ligation (BDL). The results obtained by the new technique were compared with that using Ishak fibrosis scores and two currently used quantitative methods for determining liver fibrosis: the collagen proportionate area (CPA) and measurement of hydroxyproline (HYP) content...
December 5, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27916134/underway-analysis-of-nanomolar-dissolved-reactive-phosphorus-in-oligotrophic-seawater-with-automated-on-line-solid-phase-extraction-and-spectrophotometric-system
#2
Jian Ma, Yuan Yuan, Dongxing Yuan
The automated in-field determination of trace phosphate (and other nutrients) is highly valuable for studying nutrient dynamics and cycling in oligotrophic oceans. Here, we report an automated portable analyzer for week-long underway analysis of nanomolar dissolved reactive phosphorus (DRP) in seawater. The method is based on classic phosphomolybdenum blue (PMB) chemistry combined with on-line solid phase extraction (SPE) and flow analysis. Under optimized conditions, the formed PMB from sample is automatically concentrated on a hydrophilic lipophilic balanced (HLB) copolymer SPE...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27913270/part-1-comparison-of-plasma-ammonia-results-from-seven-different-automated-platforms-in-use-throughout-central-australia
#3
Corey Markus, Michael Metz
INTRODUCTION: The clinical catchment area for the Metabolic service at the Women's and Children's Hospital in Adelaide, South Australia, covers nearly 2.5millionkm(2). Care of children with metabolic disorders in these remote areas is assisted from Adelaide, and at times, using plasma ammonia results from laboratories up to 3000km away. There are seven different platforms measuring plasma ammonia within this vast clinical catchment area. Hence, a correlation study was conducted to examine the relationship between plasma ammonia results from the seven different platforms in use throughout central Australia...
November 29, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27908978/potential-of-laboratory-execution-systems-less-to-simplify-the-application-of-business-process-management-systems-bpmss-in-laboratory-automation
#4
Sebastian Neubert, Bernd Göde, Xiangyu Gu, Norbert Stoll, Kerstin Thurow
Modern business process management (BPM) is increasingly interesting for laboratory automation. End-to-end workflow automation and improved top-level systems integration for information technology (IT) and automation systems are especially prominent objectives. With the ISO Standard Business Process Model and Notation (BPMN) 2.X, a system-independent and interdisciplinary accepted graphical process control notation is provided, allowing process analysis, while also being executable. The transfer of BPM solutions to structured laboratory automation places novel demands, for example, concerning the real-time-critical process and systems integration...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27906169/automated-identification-of-mouse-visual-areas-with-intrinsic-signal-imaging
#5
Ashley L Juavinett, Ian Nauhaus, Marina E Garrett, Jun Zhuang, Edward M Callaway
Intrinsic signal optical imaging (ISI) is a rapid and noninvasive method for observing brain activity in vivo over a large area of the cortex. Here we describe our protocol for mapping retinotopy to identify mouse visual cortical areas using ISI. First, surgery is performed to attach a head frame to the mouse skull (∼1 h). The next day, intrinsic activity across the visual cortex is recorded during the presentation of a full-field drifting bar in the horizontal and vertical directions (∼2 h). Horizontal and vertical retinotopic maps are generated by analyzing the response of each pixel during the period of the stimulus...
January 2017: Nature Protocols
https://www.readbyqxmd.com/read/27897454/considerations-for-standardizing-predictive-molecular-pathology-for-cancer-prognosis
#6
Michelangelo Fiorentino, Marina Scarpelli, Antonio Lopez-Beltran, Liang Cheng, Rodolfo Montironi
Molecular tests that were once ancillary to the core business of cyto-histopathology are becoming the third most relevant workload in pathology departments after histopathology/cytopathology and before autopsies. This has resulted from innovations in molecular biology techniques, which have developed at an incredibly fast pace. Areas covered: Most of the current widely used techniques in molecular pathology such as FISH, direct sequencing, pyrosequencing, and allele-specific PCR will be replaced by massive parallel sequencing that will not be considered next generation, but rather, will be considered to be current generation sequencing...
November 29, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27896144/clinical-decision-support-for-drug-related-events-moving-towards-better-prevention
#7
REVIEW
Sandra L Kane-Gill, Archita Achanta, John A Kellum, Steven M Handler
Clinical decision support (CDS) systems with automated alerts integrated into electronic medical records demonstrate efficacy for detecting medication errors (ME) and adverse drug events (ADEs). Critically ill patients are at increased risk for ME, ADEs and serious negative outcomes related to these events. Capitalizing on CDS to detect ME and prevent adverse drug related events has the potential to improve patient outcomes. The key to an effective medication safety surveillance system incorporating CDS is advancing the signals for alerts by using trajectory analyses to predict clinical events, instead of waiting for these events to occur...
November 4, 2016: World Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/27889359/elevated-liver-enzymes-emergency-department-focused-management
#8
Eric Sulava, Samuel Bergin, Brit Long, Alex Koyfman
BACKGROUND: Liver function test (LFT) abnormalities are a common problem faced by emergency physicians. This has become more common with the introduction of laboratory panels and automated routine laboratory testing. Fortunately, not all patients with irregularities in liver enzymes possess underlying pathology. This emergency medicine focused review provides a discussion of the various biochemical tests, their underlying biological basis, and an algorithmic approach to the interpretation of abnormalities...
November 23, 2016: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/27886006/semi-automated-extraction-and-characterization-of-stromal-vascular-fraction-using-a-new-medical-device
#9
Alexander Hanke, Lukas Prantl, Carina Wenzel, Michael Nerlich, Gero Brockhoff, Markus Loibl, Sebastian Gehmert
INTRODUCTION: The stem cell rich Stromal Vascular Fraction (SVF) can be harvested by processing lipo-aspirate or fat tissue with an enzymatic digestion followed by centrifugation. To date neither a standardised extraction method for SVF nor a generally admitted protocol for cell application in patients exists. A novel commercially available semi-automated device for the extraction of SVF promises sterility, consistent results and usability in the clinical routine. The aim of this work was to compare the quantity and quality of the SVF between the new system and an established manual laboratory method...
November 23, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27872822/automating-hesc-differentiation-with-3d-printing-and-legacy-liquid-handling-solutions
#10
Eric Zluhan, Kathleen Kelly, Nick LeClair, Danique Wortel, Kesley Moody
Historically, the routine use of laboratory automation solutions has been prohibitively expensive for many laboratories. As legacy hardware has begun to emerge on the secondary market, automation is becoming an increasingly affordable option to augment workflow in virtually any laboratory. To assess the utility of legacy liquid handling in stem cell differentiation, a used liquid handling robot was purchased at auction to automate a stem cell differentiation protocol that gives rise to CD14 + CD45+ mononuclear cells...
2016: MethodsX
https://www.readbyqxmd.com/read/27872505/harmonisation-of-osmolal-gap-can-we-use-a-common-formula
#11
REVIEW
Kay Weng Choy, Nilika Wijeratne, Zhong X Lu, James Cg Doery
Osmolal gap is the difference between the measured osmolality and a calculated osmolality based on the major commonly measured osmotically active particles. The perceived gap indicates the presence of unmeasured osmotically active particles. The major use of osmolal gap today is to screen for the possible presence of exogenous toxic substances in patients in an emergency department or intensive care unit. There is a long history of osmolal gap calculations and it needs to be appreciated that the uncertainty of the osmolal gap will be determined by the sum of errors in the calculated osmolality, error in measured osmolality and variability in unmeasured analytes...
August 2016: Clinical Biochemist. Reviews
https://www.readbyqxmd.com/read/27872403/biomechanical-analysis-of-weighted-ball-exercises-for-baseball-pitchers
#12
Glenn S Fleisig, Alek Z Diffendaffer, Kyle T Aune, Brett Ivey, Walter A Laughlin
BACKGROUND: Weighted-ball throwing programs are commonly used in training baseball pitchers to increase ball velocity. The purpose of this study was to compare kinematics and kinetics among weighted-ball exercises with values from standard pitching (ie, pitching standard 5-oz baseballs from a mound). HYPOTHESIS: Ball and arm velocities would be greater with lighter balls and joint kinetics would be greater with heavier balls. STUDY DESIGN: Controlled laboratory study...
November 21, 2016: Sports Health
https://www.readbyqxmd.com/read/27869118/de-novo-protein-function-prediction-using-dna-binding-and-rna-binding-proteins-as-a-test-case
#13
Sapir Peled, Olga Leiderman, Rotem Charar, Gilat Efroni, Yaron Shav-Tal, Yanay Ofran
Of the currently identified protein sequences, 99.6% have never been observed in the laboratory as proteins and their molecular function has not been established experimentally. Predicting the function of such proteins relies mostly on annotated homologs. However, this has resulted in some erroneous annotations, and many proteins have no annotated homologs. Here we propose a de-novo function prediction approach based on identifying biophysical features that underlie function. Using our approach, we discover DNA and RNA binding proteins that cannot be identified based on homology and validate these predictions experimentally...
November 21, 2016: Nature Communications
https://www.readbyqxmd.com/read/27868333/to-measure-or-not-to-measure-direct-oral-anticoagulants-before-surgery-or-invasive-procedures-comment
#14
R Selby, Z Shah, L Black, J Ongteco, S Kulkarni, D Piraino
With interest, we read a recent publication by Armando Tripodi, aimed at discussing whether direct oral anticoagulants (DOACs) should be measured prior to surgical or invasive procedures [1]. Although specific assays for the assessment of anticoagulant activity of the direct oral anticoagulants (dabigatran and the oral factor Xa inhibitors) have been developed and are easy to perform on automated coagulation analyzers [1-3], they are currently not available in the majority of Ontario laboratories. Since the APTT is readily available at most of our laboratories, we studied the effect of different dabigatran concentrations on the APTT, using six different APTT reagents and performed on three coagulation analyzer platforms...
November 21, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27864340/microfluidic-tissue-mesodissection-in-molecular-cancer-diagnostics
#15
Christine Surrette, David Shoudy, Alex Corwin, Wei Gao, Maria I Zavodszky, Stanislav L Karsten, Todd Miller, Michael J Gerdes, Nichole Wood, John R Nelson, Chris M Puleo
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive...
November 18, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27862350/establishment-of-complete-blood-count-reference-intervals-for-chinese-preschoolers
#16
Guo-Cheng Wang, Na Li, Cui Niu, Wei-Bo Ma, Zhu-Liang Wang, Hao Guo, Li-Hong Han
BACKGROUND: Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS: Three thousand eight hundred and twenty-one blood specimens from children aged 4 months to 6 years were collected and analyzed. Complete blood counts were determined by Sysmex XT-4000i Automated Hematology Analyzer. The nonparametric 2...
November 15, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27859908/an-improved-automated-type-based-method-for-area-assessment-of-wound-surface
#17
Xin Qi, Lian Ding, Wenjian Huang, Bing Wen, Xiaohui Guo, Jue Zhang
Accurate and precise wound measurements are a critical component of wound detection and assessment. Digital cameras are convenient and objective tools that are being increasingly used worldwide to assist with wound measurements and assessments. However, heterogeneous wounds and poor lighting conditions continue to be obstacles to wound area recognition. This study therefore provides an improved automated type-based wound area assessment method that is robust to lighting conditions and can distinguish between different wound tissues based on wound colors...
November 14, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27859449/cytocentrifuge-preparation-in-veterinary-cytology-a-quick-simple-and-affordable-manual-method-to-concentrate-low-cellularity-fluids
#18
Ricardo Marcos, Marta Santos, Carla Marrinhas, Carla Correia-Gomes, Mario Caniatti
BACKGROUND: Smears prepared by cytocentrifugation, the so-called cytospins, are widely used in human and veterinary cytology. However, the high cost has hampered the availability of commercial cytospin centrifuges in some veterinary clinics and laboratories. Nevertheless, cytospins are important for evaluating fluids with very low cellularity such as cerebrospinal fluid (CSF) or bronchoalveolar lavage fluid (BALF). OBJECTIVES: The aim of this study was to devise and test the use of a low-cost, in-house manual cytocentrifuge to obtain cytospin preparations...
November 9, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27856945/microfluidic-cartridges-for-automated-point-of-care-blood-cell-counting
#19
Suzanne Smith, Phophi Madzivhandila, René Sewart, Ureshnie Govender, Holger Becker, Pieter Roux, Kevin Land
Disposable, low-cost microfluidic cartridges for automated blood cell counting applications are presented in this article. The need for point-of-care medical diagnostic tools is evident, particularly in low-resource and rural settings, and a full blood count is often the first step in patient diagnosis. Total white and red blood cell counts have been implemented toward a full blood count, using microfluidic cartridges with automated sample introduction and processing steps for visual microscopy cell counting to be performed...
November 17, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27852634/calibration-and-evaluation-of-quantitative-antibody-titers-for-measles-virus-using-the-bioplex-2200
#20
Todd F Hatchette, Heidi Scholz, Shelly Bolotin, Natasha S Crowcroft, Colleen Jackson, Elizabeth McLachlan, Alberto Severini
The BioPlex 2200 (Bio-Rad Laboratories, Hercules, Ca) is a rapid, automated platform, which can screen large numbers of specimens for antibodies to measles, mumps, rubella and varicella. Although approved for producing qualitative results, in this study we have validated the test (off-label) to allow reporting of quantitative results. To do this, we used the 3(rd) anti-measles WHO standard to generate a calibration curve that allowed relative fluorescence intensity to be translated into quantitative antibody titer (Antibody Units/mL (AU/mL))...
November 16, 2016: Clinical and Vaccine Immunology: CVI
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