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https://www.readbyqxmd.com/read/28107543/quantification-of-pulmonary-fibrosis-in-a-bleomycin-mouse-model-using-automated-histological-image-analysis
#1
Jean-Claude Gilhodes, Yvon Julé, Sebastian Kreuz, Birgit Stierstorfer, Detlef Stiller, Lutz Wollin
Current literature on pulmonary fibrosis induced in animal models highlights the need of an accurate, reliable and reproducible histological quantitative analysis. One of the major limits of histological scoring concerns the fact that it is observer-dependent and consequently subject to variability, which may preclude comparative studies between different laboratories. To achieve a reliable and observer-independent quantification of lung fibrosis we developed an automated software histological image analysis performed from digital image of entire lung sections...
2017: PloS One
https://www.readbyqxmd.com/read/28107355/assessment-of-inter-expert-variability-and-of-an-automated-segmentation-method-of-40-and-60-mhz-ivus-images-of-coronary-arteries
#2
François Destrempes, Marie-Hélène Roy Cardinal, Yoshifumi Saijo, Gérard Finet, Jean-Claude Tardif, Guy Cloutier
The objectives were to compare the performance of a segmentation algorithm, based on the minimization of an uncertainty function, to delineate contours of external elastic membrane and lumen of human coronary arteries imaged with 40 and 60 MHz IVUS, and to use values of this function to delineate portions of contours with highest uncertainty. For 8 patients, 40 and 60 MHz IVUS coronary data acquired pre- and post-interventions were used, for a total of 68,516 images. Manual segmentations of contours (on 2312 images) performed by experts at three core laboratories were the gold-standards...
2017: PloS One
https://www.readbyqxmd.com/read/28095847/the-biomedical-piglet-establishing-reference-intervals-for-haematology-and-clinical-chemistry-parameters-of-two-age-groups-with-and-without-iron-supplementation
#3
Domenico Ventrella, Francesco Dondi, Francesca Barone, Federica Serafini, Alberto Elmi, Massimo Giunti, Noemi Romagnoli, Monica Forni, Maria L Bacci
BACKGROUND: The similarities between swine and humans in physiological and genomic patterns, and the great correlation in size and anatomy, make pigs extremely useful in preclinical studies. New-born piglets can represent a model for congenital and genetic diseases in new-born children. It is known that piglets may have significant differences in clinicopathological results compared to adult pigs. Therefore, adult laboratory reference intervals cannot be applied to piglets. The aim of this study was to compare haematological and chemical variables in piglets of two ages and determinate age-related reference intervals for commercial hybrid young pigs...
January 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28087144/-contribution-of-microbiology-in-the-diagnosis-of-tuberculosis-in-castile-and-le%C3%A3-n-spain-findings-of-the-grumicale-2013-study
#4
Ramiro López-Medrano, Teresa Nebreda-Mayoral, M Fé Brezmes-Valdivieso, Susana García-de Cruz, Begoña Nogueira-González, Rafael Sánchez-Arroyo, Almudena Tinajas-Puertas, Nieves Gutiérrez-Zufiaurre, Cristina Labayru-Echeverría, Susana Hernando-Real, Luis López-Urrutia, Octavio Rivero-Lezcano, Belén Ullivarri-Francia, Raquel Rodríguez-Tarazona, Isabel Antolín-Ayala
INTRODUCTION AND OBJECTIVES: A retrospective study was conducted by collecting microbiological tuberculosis (TB) data in Castile and León during the year 2013 in order to determine the incidence and distribution of TB, and resistance to the tuberculostatic drug, and compare them with the epidemiological data provided by the Department of Epidemiological Surveillance (SIVE). MATERIAL AND METHODS: Microbiologists of the 14 hospitals of the Castile and León public health network (GRUMICALE) collected epidemiological, microbiological, and management data from the Microbiology laboratories in the community during the year 2013...
January 10, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28086955/evaluation-of-the-xpert%C3%A2-mtb-rif-assay-and-microscopy-for-the-diagnosis-of-mycobacterium-tuberculosis-in-namibia
#5
Rooyen T Mavenyengwa, Emma Shaduka, Innocent Maposa
BACKGROUND: Tuberculosis (TB) kills approximately two million people and infects around nine million worldwide annually. Its proper management, especially in resource-limited settings, has been hindered by the lack of rapid and easy-to-use diagnostic tests. Sputum smear microscopy remains the cheapest, readily available diagnostic method but it only identifies less than half of the patients with a HIV/TB co-infection because the bacilli would have disseminated from the lungs to other areas of the body...
January 11, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28078809/three-year-analysis-of-repeated-laboratory-tests-for-the-markers-total-cholesterol-ferritin-vitamin-d-vitamin-b12-and-folate-in-a-large-research-and-teaching-hospital-in-italy
#6
Monica Lanzoni, Marco Fornili, Irene Felicetta, Rita Maiavacca, Elia Biganzoli, Silvana Castaldi
RATIONALE, AIMS AND OBJECTIVES: The increasing number of diagnostic tests requests all over the world is a problem that can partially be explained by inappropriate testing. Impact on the total costs of health systems becomes relevant when tests are performed in a large amount. In this paper, retesting of total cholesterol, ferritin, vitamin B12 , vitamin D, and folate is assessed. METHODS: The Quality Unit of Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico di Milano (Fondazione) decided to perform a first assessment of the appropriate use of the laboratory tests cholesterol, ferritin, vitamin B12 , vitamin D, and folate focusing on the retesting interval for the same patient in the time period January 1, 2012, to December 31, 2014, in every care setting...
January 12, 2017: Journal of Evaluation in Clinical Practice
https://www.readbyqxmd.com/read/28076305/analytical-and-diagnostic-performance-of-two-automated-fecal-calprotectin-immunoassays-for-detection-of-inflammatory-bowel-disease
#7
Maxime M W De Sloovere, Dieter De Smet, Filip J Baert, Johan Debrabandere, Hilde J M Vanpoucke
BACKGROUND: We evaluated the (pre-)analytical and diagnostic performance of two automated fecal calprotectin (FC) immunoassays, Liaison® Calprotectin (Diasorin) on Liaison® XL and fCAL™ turbo (Bühlmann laboratories AG) on Cobas C501 (Roche Diagnostics), and compared it with our established Bühlmann ELISA method. METHODS: Our study comprised 229 consecutive patients with clinical suspicion of inflammatory bowel disease (IBD). RESULTS: All assay related stool extraction procedures showed excellent correlation with the established method, but the new stool extraction devices tend to give higher results as compared with stool weight methods...
January 11, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28068803/annals-express-artifactual-25-oh-vitamin-d-level-in-multiple-myeloma
#8
Michal Wen Sheue Ong, Rashim Salota, Tracy Reeman, Lapsley Marta, Lydia Jones
The most commonly used techniques to measure vitamin D are automated immunoassays which are known to be affected by interferences, especially from immunoglobulins present in the patient's serum. We present a case of a patient with myeloma in whom interference with the vitamin D assay was identified. An eighty-three year old female, known to have IgG myeloma was found to have a high concentration of 25-OH vitamin D on a routine test without any signs of vitamin D toxicity. She was not taking vitamin D supplements or any other multivitamin preparation and had minimal sun exposure...
January 1, 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28062104/assessment-of-a-novel-device-for-onsite-integrative-large-volume-solid-phase-extraction-of-water-samples-to-enable-a-comprehensive-chemical-and-effect-based-analysis
#9
Tobias Schulze, Marijan Ahel, Jörg Ahlheim, Selim Aït-Aïssa, François Brion, Carolina Di Paolo, Jean Froment, Anita O Hidasi, Juliane Hollender, Henner Hollert, Meng Hu, Anett Kloß, Sanja Koprivica, Martin Krauss, Melis Muz, Peter Oswald, Margit Petre, Jennifer E Schollée, Thomas-Benjamin Seiler, Ying Shao, Jaroslav Slobodnik, Manoj Sonavane, Marc J-F Suter, Knut Erik Tollefsen, Zuzana Tousova, Karl-Heinz Walz, Werner Brack
The implementation of targeted and nontargeted chemical screening analysis in combination with in vitro and organism-level bioassays is a prerequisite for a more holistic monitoring of water quality in the future. For chemical analysis, little or no sample enrichment is often sufficient, while bioanalysis often requires larger sample volumes at a certain enrichment factor for conducting comprehensive bioassays on different endpoints or further effect-directed analysis (EDA). To avoid logistic and technical issues related to the storage and transport of large volumes of water, sampling would benefit greatly from onsite extraction...
January 3, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28060883/an-automated-experimenter-free-method-for-the-standardised-operant-cognitive-testing-of-rats
#10
Marion Rivalan, Humaira Munawar, Anna Fuchs, York Winter
Animal models of human pathology are essential for biomedical research. However, a recurring issue in the use of animal models is the poor reproducibility of behavioural and physiological findings within and between laboratories. The most critical factor influencing this issue remains the experimenter themselves. One solution is the use of procedures devoid of human intervention. We present a novel approach to experimenter-free testing cognitive abilities in rats, by combining undisturbed group housing with automated, standardized and individual operant testing...
2017: PloS One
https://www.readbyqxmd.com/read/28060280/a-protocol-of-manual-tests-to-measure-sensation-and-pain-in-humans
#11
Matthew Kostek, Anna Polaski, Benedict Kolber, Austin Ramsey, Alexander Kranjec, Kimberly Szucs
Numerous qualitative and quantitative techniques can be used to test sensory nerves and pain in both research and clinical settings. The current study demonstrates a quantitative sensory testing protocol using techniques to measure tactile sensation and pain threshold for pressure and heat using portable and easily accessed equipment. These techniques and equipment are ideal for new laboratories and clinics where cost is a concern or a limiting factor. We demonstrate measurement techniques for the following: cutaneous mechanical sensitivity on the arms and legs (von-Frey filaments), radiant and contact heat sensitivity (with both threshold and qualitative assessments using the Visual Analog Scale (VAS)), and mechanical pressure sensitivity (algometer, with both threshold and the VAS)...
December 19, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28053214/the-bactec-fx-blood-culture-system-detects-brucella-melitensis-bacteremia-in-adult-patients-within-the-routine-one-week-incubation-period
#12
Moshe Sagi, Lior Nesher, Pablo Yagupsky
The performance of the BACTEC FX blood culture system for detecting Brucella bacteremia within the routine one-week incubation period was assessed in a prospective study conducted in an endemic area for B. melitensis in southern Israel. Aerobic vials (BD BACTEC™ Plus Aerobic/F Medium) inoculated with blood specimens obtained from adult patients with a positive Rose-Bengal screening test were monitored for four consecutive weeks and blind subcultures of negative vials were performed on solid media on days 7 and 28...
January 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28052923/primerize-2d-automated-primer-design-for-rna-multidimensional-chemical-mapping
#13
Siqi Tian, Rhiju Das
Rapid RNA synthesis of comprehensive single mutant libraries and targeted multiple mutant libraries is enabling new multidimensional chemical approaches to solve RNA structures. PCR assembly of DNA templates and in vitro transcription allows synthesis and purification of hundreds of RNA mutants in a cost-effective manner, with sharing of primers across constructs allowing significant reductions in expense. However, these protocols require organization of primer locations across numerous 96 well plates and guidance for pipetting, non-trivial tasks for which informatics and visualization tools can prevent costly errors...
January 3, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28046040/impact-of-pre-analytical-time-on-the-recovery-of-pathogens-from-blood-cultures-results-from-a-large-retrospective-survey
#14
Claudia Venturelli, Elena Righi, Lucia Borsari, Gabriella Aggazzotti, Stefano Busani, Cristina Mussini, Fabio Rumpianesi, Gian Maria Rossolini, Massimo Girardis
BACKGROUND: Prompt identification of bloodstream pathogens is essential for optimal management of patients. Significant changes in analytical methods have improved the turnaround time for laboratory diagnosis. Less attention has been paid to the time elapsing from blood collection to incubation and to its potential effect on recovery of pathogens. We evaluated the performance of blood cultures collected under typical hospital conditions in relation to the length of their pre-analytical time...
2017: PloS One
https://www.readbyqxmd.com/read/28043626/comparison-of-two-molecular-methods-and-an-automated-liquid-culture-system-for-the-early-detection-of-mycobacterium-tuberculosis-from-both-pulmonary-and-extrapulmonary-specimens-in-kuwait
#15
E M Mokaddas, H Saadaldeen, S Ahmad
OBJECTIVE/BACKGROUND: Molecular methods for the detection of Mycobacterium tuberculosis (MTB) are widely used for the early diagnosis of tuberculosis (TB). The objectives of this study were to evaluate the performance of two molecular techniques for the detection of MTB for both pulmonary and extrapulmonary specimens in comparison with conventional culture techniques in Kuwait and to correlate the results with clinical and histopathological diagnosis. METHODS: A total of 9,594 consecutive pulmonary (n=5,597) and extrapulmonary (n=3,997) specimens received by the Kuwait National TB Reference Laboratory from November 2011 to December 2015 were processed for direct smear microscopy for acid-fast bacilli using a Zhiel-Neelsen stain, Xpert MTB/RIF assay, ProbTec polymerase chain reaction (PCR), and growth in fully automated MGIT 960 system tubes...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#16
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043590/use-of-wgs-in-mycobacterium-tuberculosis-routine-diagnosis
#17
Daniela M Cirillo, Andrea M Cabibbe, Maria Rosaria De Filippo, Alberto Trovato, Tullia Simonetti, Gian Maria Rossolini, Enrico Tortoli
OBJECTIVE/BACKGROUND: Whole Genome Sequencing (WGS) is becoming affordable with overall costs comparable to other tests currently in use to perform the diagnosis of drug-resistant tuberculosis (TB) and cluster analysis. The WGS approach allows an "all-in-one" approach providing results on expected sensitivity of the strains, genetic background, epidemiological data, and indication of risk of laboratory cross-contamination. METHODS: Although ideal, WGS from the direct diagnostic specimen is not yet standardized, and to date the two most promising approaches are WGS from early positive liquid culture and targeted sequencing from diagnostic specimens using Next-Generation Technology...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043528/childhood-tuberculosis-in-qatar
#18
Jay P N Singh, Reem Mohammed, Thanveer Thayyullathil, Abdullatif Al-Khal, Zubaida Al-Suwaidi
Childhood tuberculosis (TB) has been long neglected but has gained attention in recent years. In 2012, the World Health Organization annual report included an estimate for childhood TB for the first time, and in the following year, the TB Alliance received a grant from UNITAID (International Drug Purchase Facility) to develop pediatric TB formulations. Qatar is a low-incidence country. In this observational study, laboratory-confirmed cases of TB were analyzed from 2013 to 2015 and included patients aged ⩽14years...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043179/the-cobas%C3%A2-6800-8800-system-a-new-era-of-automation-in-molecular-diagnostics
#19
Bryan Cobb, Christian O Simon, Susan L Stramer, Barbara Body, P Shawn Mitchell, Natasa Reisch, Wendy Stevens, Sergio Carmona, Louis Katz, Stephen Will, Oliver Liesenfeld
Molecular diagnostics is a key component of laboratory medicine. Here, the authors review key triggers of ever-increasing automation in nucleic acid amplification testing (NAAT) with a focus on specific automated Polymerase Chain Reaction (PCR) testing and platforms such as the recently launched cobas® 6800 and cobas® 8800 Systems. The benefits of such automation for different stakeholders including patients, clinicians, laboratory personnel, hospital administrators, payers, and manufacturers are described...
January 3, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28041870/the-effect-of-nucleic-acid-extraction-platforms-and-sample-storage-on-the-integrity-of-viral-rna-for-use-in-whole-genome-sequencing
#20
Kuiama Lewandowski, Andrew Bell, Rory Miles, Simon Carne, David Wooldridge, Carmen Manso, Nicola Hennessy, Daniel Bailey, Steven T Pullan, Saheer Gharbia, Richard Vipond
Extraction of viral RNA and the storage of sample material are extremely important factors in the detection and whole genome sequencing (WGS) of viral pathogens. Although PCR-based detection methods focus on small amplicons, viral WGS applications require RNA of high quality and integrity for adequate sequence coverage and depth. This study examined the fitness of one manual and four automated RNA extraction platforms commonly used in diagnostic laboratories for use in metagenomic sequencing, how the practice of storing sample material in Qiagen buffer AVL before extraction affected the integrity of viral RNA and its suitability for use in amplicon-based WGS methods, and how the addition of Triton X-100 to buffer AVL affected the capability of the extraction platforms and the integrity of viral RNA in stored samples...
December 29, 2016: Journal of Molecular Diagnostics: JMD
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