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https://www.readbyqxmd.com/read/29333150/evaluation-of-the-correlation-coefficient-of-polyethylene-glycol-treated-and-direct-prolactin-results-and-comparability-with-different-assay-system-results
#1
Shyamali Pal
The presence of Macro prolactin is a significant cause of elevated prolactin resulting in misdiagnosis in all automated systems. Poly ethylene glycol (PEG) pretreatment is the preventive process but such process includes the probability of loss of a fraction of bioactive prolactin. Surprisingly, PEG treated EQAS & IQAS samples in Cobas e 411 are found out to be correlating with direct results of at least 3 immunoassay systems and treated and untreated Cobas e 411 results are comparable by a correlation coefficient...
December 2017: EJIFCC
https://www.readbyqxmd.com/read/29333146/cystatin-c-is-indispensable-for-evaluation-of-kidney-disease
#2
Anders Grubb
The present minireview of the place of cystatin C in clinical medicine emphasizes, and discuss the evidence, that cystatin C-based GFR-estimating equations do not require the use of vague terms like race and sex, that cystatin C-based GFR-esti mating equations are useful for both children and adults, including the elderly, that the best GFR-estimation requires simultaneous use of both cystatin C- and creatinine-based equations, that cystatin C-based GFR-estimating equations are superior to creatinine-based equations in predicting end-stage renal disease, cardiovascular manifestations, hospitalisation and death, and, finally that cystatin C is required to diagnose the new syndrome "Shrunken Pore Syndrome" with its high mortality and morbidity, even in the absence of reduced GFR...
December 2017: EJIFCC
https://www.readbyqxmd.com/read/29328964/a-european-multicenter-study-on-the-analytical-performance-of-the-veris-hbv-assay
#3
Patrick Braun, Rafael Delgado, Monica Drago, Diana Fanti, Hervé Fleury, Jacques Izopet, Alessandra Lombardi, Alessandro Mancon, Maria Angeles Marcos, Karine Sauné, Siobhan O Shea, Alfredo Pérez-Rivilla, John Ramble, Pascale Trimoulet, Jordi Vila, Duncan Whittaker, Alain Artus, Daniel Rhodes
BACKGROUND: Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostics System.1 OBJECTIVES: To evaluate the analytical performance of the VERIS HBV Assay at multiple European virology laboratories. STUDY DESIGN: Precision, analytical sensitivity, negative sample performance, linearity and performance with major HBV genotypes/subtypes for the VERIS HBV Assay was evaluated...
December 19, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29324744/qcloud-a-cloud-based-quality-control-system-for-mass-spectrometry-based-proteomics-laboratories
#4
Cristina Chiva, Roger Olivella, Eva Borràs, Guadalupe Espadas, Olga Pastor, Amanda Solé, Eduard Sabidó
The increasing number of biomedical and translational applications in mass spectrometry-based proteomics poses new analytical challenges and raises the need for automated quality control systems. Despite previous efforts to set standard file formats, data processing workflows and key evaluation parameters for quality control, automated quality control systems are not yet widespread among proteomics laboratories, which limits the acquisition of high-quality results, inter-laboratory comparisons and the assessment of variability of instrumental platforms...
2018: PloS One
https://www.readbyqxmd.com/read/29320362/predictive-value-of-serum-gelsolin-and-gc-globulin-in-sepsis-a-pilot-study
#5
Zoltán Horváth-Szalai, Péter Kustán, Balázs Szirmay, Ágnes Lakatos, Per Hjort Christensen, Tamás Huber, Beáta Bugyi, Diána Mühl, Andrea Ludány, Attila Miseta, Gábor L Kovács, Tamás Kőszegi
BACKGROUND: Simultaneous determination of the two main actin scavenger proteins in sepsis has not been investigated until now. In our pilot study, we elucidated the predictive values of Gc globulin and gelsolin (GSN) in sepsis by comparing them to classic laboratory and clinical parameters. METHODS: A 5-day follow-up was performed, including 46 septic patients, 28 non-septic patients and 35 outpatients as controls. Serum Gc globulin and GSN levels were determined by automated immune turbidimetric assay on a Cobas 8000/c502 analyzer...
January 10, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29314756/sorting-specimen-rich-invertebrate-samples-with-cost-effective-ngs-barcodes-validating-a-reverse-workflow-for-specimen-processing
#6
Wendy Y Wang, Amrita Srivathsan, Maosheng Foo, Seiki Yamane, Rudolf Meier
Biologists frequently sort specimen-rich samples to species. This process is daunting when based on morphology, and disadvantageous if performed using molecular methods that destroy vouchers (e.g., metabarcoding). An alternative is barcoding every specimen in a bulk sample and then presorting the specimens using DNA barcodes, thus mitigating downstream morphological work on presorted units. Such a "reverse workflow" is too expensive using Sanger sequencing, but we here demonstrate that is feasible with an NGS barcoding pipeline that allows for cost-effective high throughput generation of short specimen-specific barcodes (313 bp of COI; lab cost <$0...
January 5, 2018: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29314650/validation-of-reflex-testing-rules-and-establishment-of-a-new-workflow-for-body-fluid-cell-analysis-using-a-sysmex-xn-550-automatic-hematology-analyzer
#7
Y-U Cho, E You, S Jang, C-J Park
INTRODUCTION: We developed and validated reflex testing rules for the microscopic examination (ME) of body fluids (BFs) on the Sysmex XN-550 (Sysmex Corporation) instrument. METHODS: We assessed the detection limits, precision, linearity, and carryover. To develop the reflex testing rules (derivation arm), we tested 515 samples and then validated the rules using another 507 samples (validation arm). RESULTS: All analytical performances were acceptable, and the carryover was negligible...
January 4, 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29314254/manual-versus-automated-streaking-system-in-clinical-microbiology-laboratory-performance-evaluation-of-previ-isola-for-blood-culture-and-body-fluid-samples
#8
Qute Choi, Hyun Jin Kim, Jong Wan Kim, Gye Cheol Kwon, Sun Hoe Koo
BACKGROUND: The process of plate streaking has been automated to improve routine workflow of clinical microbiology laboratories. Although there were many evaluation reports about the inoculation of various body fluid samples, few evaluations have been reported for blood. In this study, we evaluated the performance of automated inoculating system, Previ Isola for various routine clinical samples including blood. METHODS: Blood culture, body fluid, and urine samples were collected...
January 4, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29313570/fosfomycin-susceptibility-in-urinary-tract-enterobacteriaceae
#9
Bhargav Patel, Kinjal Patel, Anjali Shetty, Rajeev Soman, Camilla Rodrigues
Introduction: Antibiotic treatment of Urinary Tract Infections (UTI) is becoming increasingly difficult due to emergence of multi-drug resistant (ESBLs, AmpC, CRE) uropathogens. Fosfomycin is an old antibiotic that has evoked renewed interest with unique properties of not sharing any structural similarity and lack of cross-resistance with other antimicrobial agents. Our aim is to evaluate in-vitro activity of Fosfomycin against urinary tract Enterobacteriaceae. Material & Methods: The study period was March 2014 to September 2015...
September 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/29310948/evaluation-of-the-diasorin-liaison%C3%A2-xl-zika-capture-igm-cmia-for-zika-virus-serological-testing
#10
Angela Sloan, David Safronetz, Kai Makowski, Nicole Barairo, Charlene Ranadheera, Kristina Dimitrova, Kimberly Holloway, Emelissa Mendoza, Heidi Wood, Mike Drebot, Ainsley Gretchen, Kamran Kadkhoda
Due to the increase of Zika virus (ZIKV) transmission throughout the world, many commercial kits have recently become available to aid in laboratory diagnosis of ZIKV infections in clinical samples. Here, we analyze the fully automated Liaison® XL Zika Capture immunoglobulin M (IgM) assay against the recommended IgM-capture enzyme-linked immunosorbent assay.
December 5, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29310696/methods-for-detecting-gemmata-spp-bacteremia-in-the-microbiology-laboratory
#11
Jacques-Robert Christen, Edwin Edmond, Michel Drancourt
OBJECTIVE: Gemmata bacteria are fastidious, Gram-negative and aerobic. The only representatives are Gemmata obscuriglobus and Gemmata massiliana. These Planctomycetes appear to be a part of human digestive tract microbiome, and G. massiliana has been isolated from water. Further specific detection in the blood of two patients with febrile neutropenia suggests that Gemmata bacteremia may remain under-documented. The objective of this study was to develop an effective protocol to document Gemmata spp...
January 8, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29306072/improvements-and-cost-effective-measures-to-the-automated-intermittent-water-renewal-system-for-toxicity-testing-with-sediments
#12
W Tyler Mehler, Jing You, Michael J Keough, Michael J Lydy, Vincent Pettigrove
The push to make bioassays more sensitive has meant an increased duration of testing to look at more chronic endpoints. To conduct these longer bioassays through the use of traditional bioassay methods can be difficult, as many traditional bioassays have employed manual water changes, which take considerable time and effort. To that end, static-renewal systems were designed to provide researchers a technique to ease the manual water change burden. One of the most well-known static-renewal designs, the static intermittent renewal system (STIR) was produced by the United States Environmental Protection Agency in 1993...
January 3, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29305540/direct-from-blood-culture-disk-diffusion-to-determine-antimicrobial-susceptibility-of-gram-negative-bacteria-preliminary-report-from-the-clinical-and-laboratory-standards-institute-methods-development-and-standardization-working-group
#13
Sukantha Chandrasekaran, April Abbott, Shelley Miller, Barb Zimmer, Mel Weinstein, Lauri Thrupp, John Hejna, Lindsey Walker, Tracy Ammann, Thomas Kirn, Robin Patel, Romney M Humphries
The performance of disk diffusion, using broth from positive blood cultures as inoculum ("direct disk diffusion", dDD), was evaluated for a collection of 20 challenge isolates of Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa. Isolates seeded into human blood were inoculated into BACTEC™ Plus Aerobic/F, VersaTREK REDOX 1 and BacT/ALERT FA Plus bottles and incubated on the respective automated blood culture systems. Disk diffusion results were compared to reference disk diffusion. Categorical agreement (CA) of dDD, after removal of random errors due to natural MIC variation, was 87...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29304138/the-use-of-automated-ki67-analysis-to-predict-oncotype-dx-risk-of-recurrence-categories-in-early-stage-breast-cancer
#14
Satbir Singh Thakur, Haocheng Li, Angela M Y Chan, Roxana Tudor, Gilbert Bigras, Don Morris, Emeka K Enwere, Hua Yang
Ki67 is a commonly used marker of cancer cell proliferation, and has significant prognostic value in breast cancer. In spite of its clinical importance, assessment of Ki67 remains a challenge, as current manual scoring methods have high inter- and intra-user variability. A major reason for this variability is selection bias, in that different observers will score different regions of the same tumor. Here, we developed an automated Ki67 scoring method that eliminates selection bias, by using whole-slide analysis to identify and score the tumor regions with the highest proliferative rates...
2018: PloS One
https://www.readbyqxmd.com/read/29300769/validation-of-an-automated-system-for-aliquoting-of-hiv-1-env-pseudotyped-virus-stocks
#15
Anke Schultz, Anja Germann, Martina Fuss, Marcella Sarzotti-Kelsoe, Daniel A Ozaki, David C Montefiori, Heiko Zimmermann, Hagen von Briesen
The standardized assessments of HIV-specific immune responses are of main interest in the preclinical and clinical stage of HIV-1 vaccine development. In this regard, HIV-1 Env-pseudotyped viruses play a central role for the evaluation of neutralizing antibody profiles and are produced according to Good Clinical Laboratory Practice- (GCLP-) compliant manual and automated procedures. To further improve and complete the automated production cycle an automated system for aliquoting HIV-1 pseudovirus stocks has been implemented...
2018: PloS One
https://www.readbyqxmd.com/read/29296502/automated-multimodal-spectral-histopathology-for-quantitative-diagnosis-of-residual-tumour-during-basal-cell-carcinoma-surgery
#16
Radu Boitor, Kenny Kong, Dustin Shipp, Sandeep Varma, Alexey Koloydenko, Kusum Kulkarni, Somaia Elsheikh, Tom Bakker Schut, Peter Caspers, Gerwin Puppels, Martin van der Wolf, Elena Sokolova, T E C Nijsten, Brogan Salence, Hywel Williams, Ioan Notingher
Multimodal spectral histopathology (MSH), an optical technique combining tissue auto-fluorescence (AF) imaging and Raman micro-spectroscopy (RMS), was previously proposed for detection of residual basal cell carcinoma (BCC) at the surface of surgically-resected skin tissue. Here we report the development of a fully-automated prototype instrument based on MSH designed to be used in the clinic and operated by a non-specialist spectroscopy user. The algorithms for the AF image processing and Raman spectroscopy classification had been first optimised on a manually-operated laboratory instrument and then validated on the automated prototype using skin samples from independent patients...
December 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29287260/design-and-validation-of-an-automated-solid-phase-extraction-liquid-chromatography-coupled-mass-spectrometry-method-for-the-quantification-of-propofol-in-plasma
#17
Felix Maurer, Teodora Shopova, Beate Wolf, Daniel Kiefer, Tobias Hüppe, Thomas Volk, Daniel I Sessler, Sascha Kreuer
Propofol concentration in human plasma can be quantified by liquid chromatography coupled mass spectrometry. Sample preparation usually requires solid phase extraction to remove matrix components and enrich the analyte. To facilitate user-independent measurements and speed extraction, we developed and validated a fully automated high throughput in-line sample preparation system with direct injection into liquid chromatography coupled mass spectrometry. We assessed linearity of each method over the clinically relevant concentration range from 0...
December 21, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29287094/validation-of-a-novel-automatic-deposition-of-bacteria-and-yeasts-on-maldi-target-for-maldi-tof-ms-based-identification-using-maldi-colonyst-robot
#18
Katerina Chudejova, Michal Bohac, Anna Skalova, Veronika Rotova, Costas C Papagiannitsis, Jana Hanzlickova, Tamara Bergerova, Jaroslav Hrabák
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic acid and by automatic deposition using MALDI Colonyst...
2017: PloS One
https://www.readbyqxmd.com/read/29286417/building-up-a-high-throughput-screening-platform-to-assess-the-heterogeneity-of-her2-gene-amplification-in-breast-cancers
#19
Giulia Ercoli, Gianluca Lopez, Camilla Ciapponi, Chiara Corti, Luca Despini, Donatella Gambini, Letterio Runza, Concetta Blundo, Amedeo Sciarra, Nicola Fusco
Targeted therapies against the human epidermal growth factor receptor 2 (HER2) have radically changed the outcome of patients with HER2-positive breast cancers. However, a minority of cases displays a heterogeneous distribution of HER2-positive cells, which generates major clinical challenges. To date, no reliable and standardized protocols for the characterization and quantification of HER2 heterogeneous gene amplification in large cohorts have been proposed. Here, we present a high-throughput methodology to simultaneously assess the HER2 status across different topographic areas of multiple breast cancers...
December 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29282212/musclemotion-a-versatile-open-software-tool-to-quantify-cardiomyocyte-and-cardiac-muscle-contraction-in-vitro-and-in-vivo
#20
Luca Sala, Berend J van Meer, Leon T Tertoolen, Jeroen Bakkers, Milena Bellin, Richard P Davis, Chris N Denning, Michel A Dieben, Thomas Eschenhagen, Elisa Giacomelli, Catarina Grandela, Arne Hansen, Eduard Holman, Monique R Jongbloed, Sarah M Kamel, Charlotte D Koopman, Quentin Lachaud, Ingra Mannhardt, Mervyn P Mol, Diogo Mosqueira, Valeria V Orlova, Robert Passier, Marcelo C Ribeiro, Umber Saleem, Godfrey Smith, Francis L L Burton, Christine L Mummery
Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Consequently, data and techniques have been difficult to reproduce across models and laboratories, analysis is time consuming and only specialist researchers can quantify data. Objective: Here we describe and validate an automated, open source software tool (MUSCLEMOTION) adaptable for use with standard laboratory- and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes and pharmacological responses...
December 27, 2017: Circulation Research
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