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https://www.readbyqxmd.com/read/28522403/multiplex-autoantibody-detection-for-autoimmune-liver-diseases-and-autoimmune-gastritis
#1
Joris Vanderlocht, Mart van der Cruys, Frans Stals, Liesbeth Bakker-Jonges, Jan Damoiseaux
Autoantibody detection for autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC) and autoimmune gastritis (AIG) is traditionally performed by IIF on a combination of tissues. Multiplex line/dot blots (LIA/DIA) offer multiple advantages, i.e. automation, objective reading, no interfering reactivities, no coincidental findings. In the current study we evaluated automated DIA (D-Tek) for detecting autoantibodies related to autoimmune diseases of the gastrointestinal tract. We tested samples of the Dutch EQC program and compared the results with the consensus of the participating labs...
May 15, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28520977/asynchronous-automated-electronic-laboratory-result-notifications-a-systematic-review
#2
Benjamin H Slovis, Thomas A Nahass, Hojjat Salmasian, Gilad Kuperman, David K Vawdrey
Objective: To systematically review the literature pertaining to asynchronous automated electronic notifications of laboratory results to clinicians. Methods: PubMed, Web of Science, and the Cochrane Collaboration were queried for studies pertaining to automated electronic notifications of laboratory results. A title review was performed on the primary results, with a further abstract review and full review to produce the final set of included articles. Results: The full review included 34 articles, representing 19 institutions...
May 18, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28520873/association-between-neurocognitive-decline-and-visual-field-variability-in-glaucoma
#3
Alberto Diniz-Filho, Lisa Delano-Wood, Fábio B Daga, Sebastião Cronemberger, Felipe A Medeiros
Importance: Visual field variability may impair detection of glaucoma progression over time. Despite the possible overlap between neurocognitive disorders and glaucoma in older individuals, no study has investigated the association between cognitive changes and visual field variability. Objective: To evaluate the association between global neurocognitive impairment and visual field variability in patients diagnosed as having glaucoma or glaucoma suspects. Design, Setting, and Participants: This prospective observational cohort study was conducted at the Visual Performance Laboratory, University of California, San Diego...
May 18, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28513101/industrialization-of-a-perfusion-bioreactor-prime-example-of-a-non-straightforward-process
#4
G Talò, C Turrisi, C Arrigoni, C Recordati, I Gerges, M Tamplenizza, A Cappelluti, S A Riboldi, M Moretti
Bioreactors are essential enabling technologies for the translation of Advanced Therapies Medicinal Products (ATMPs) from the research field towards a successful clinical application. In order to speed up the translation and the spreading of novel Tissue Engineering (TE) products into the clinical routine, TE bioreactors should evolve from laboratory prototypes towards industrialized products. In this work, we thus challenged the industrialization process of a novel technological platform, based on an established research prototype of perfusion bioreactor, following a GMP-driven approach...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28504684/fully-automated-5-plex-fluorescent-immunohistochemistry-with-tyramide-signal-amplification-and-same-species-antibodies
#5
Wenjun Zhang, Antony Hubbard, Tobin Jones, Adriana Racolta, Srabani Bhaumik, Nick Cummins, Liping Zhang, Karl Garsha, Frank Ventura, Mark R Lefever, Zhenqiang Lu, John K Hurley, William A Day, Lidija Pestic-Dragovich, Larry E Morrison, Lei Tang
The ability to simultaneously visualize the presence, abundance, location and functional state of many targets in cells and tissues has been described as a true next-generation approach in immunohistochemistry (IHC). A typical requirement for multiplex IHC (mIHC) is the use of different animal species for each primary (1°Ab) and secondary (2°Ab) antibody pair. Although 1°Abs from different species have been used with differently labeled species-specific 2°Abs, quite often the appropriate combination of antibodies is not available...
May 15, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28502701/fastpcr-an-in-silico-tool-for-fast-primer-and-probe-design-and-advanced-sequence-analysis
#6
Ruslan Kalendar, Bekbolat Khassenov, Erlan Ramanculov, Olga Samuilova, Konstantin I Ivanov
Polymerase chain reaction (PCR) is one of the most important laboratory techniques used in molecular biology, genetics and molecular diagnostics. The success of a PCR-based method largely depends on the correct nucleic acid sequence analysis in silico prior to a wet-bench experiment. Here, we report the development of an online Java-based software for virtual PCR on linear or circular DNA templates and multiple primer or probe search from large or small databases. Primer or probe sensitivity and specificity are predicted by searching a database to find sequences with an optimal number of mismatches, similarity and stability...
May 11, 2017: Genomics
https://www.readbyqxmd.com/read/28502007/high-resolution-image-stitching-as-a-tool-to-assess-tissue-level-protein-distribution-and-localization
#7
Bryan A Millis, Matthew J Tyska
High-resolution microscopy has traditionally come at the expense of field of view, resulting in suboptimal interpretation of protein distribution throughout large or complex samples. Likewise, a low-resolution microscopic approach inhibits the ability of researchers to precisely localize proteins of interest at the subcellular level. Until recently, the ability to combine the strengths of these approaches was limited and technically impractical for most laboratories to implement. Continued advances in microscope automation, sophisticated software applications, and modern workstations have enabled expansion of such combinatorial approaches to researchers outside computationally focused fields...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28501993/antibody-validation-by-western-blotting
#8
Michele Signore, Valeria Manganelli, Alex Hodge
Validation of antibodies is an integral part of translational research, particularly for biomarker discovery. Assaying the specificity of the reagent (antibody) and confirming the identity of the protein biomarker is of critical importance prior to implementing any biomarker in clinical studies, and the lack of such quality control tests may result in unexpected and/or misleading results.Antibody validation is the procedure in which a single antibody is thoroughly assayed for sensitivity and specificity. Although a plethora of commercial antibodies exist, antibody specificity must be extensively demonstrated using diverse complex biological samples, rather than purified recombinant proteins, prior to use in clinical translational research...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28500649/a-comparative-study-using-thrombin-generation-and-three-different-inr-methods-in-patients-on-vitamin-k-antagonist-treatment
#9
N Riva, K Vella, S Meli, K Hickey, D Zammit, C Calamatta, M Makris, S Kitchen, W Ageno, A Gatt
INTRODUCTION: Vitamin K antagonist (VKA) treatment requires routine monitoring using the international normalized ratio (INR). However, different INR assays may vary in their results. The aim of this study was to assess the agreement of three different INR methods, compared with thrombin generation, in patients on VKA treatment. METHODS: Sixty patients attending the Anticoagulation Clinic at Mater Dei Hospital (Msida, Malta) for VKA monitoring between August and September 2015 were enrolled...
May 12, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28490046/rapid-homogeneous-immunoassay-to-quantify-gemcitabine-in-plasma-for-therapeutic-drug-monitoring
#10
Daniel Kozo, Matt W Ross, Justin Jarrah, Michael Barrett, Rebecca L Harney, Jodi B Courtney, Irina Baburina, Julianne L Holleran, Jan H Beumer, Godefridus J Peters, Richard J Honeywell, Salvatore J Salamone
BACKGROUND: Gemcitabine (2',2'-difluoro-2'-deoxycytidine) is a nucleoside analog used as a single agent and in combination regimens for the treatment of a variety of solid tumors. Several studies have shown a relationship between gemcitabine peak plasma concentration (Cmax) and hematological toxicity. An immunoassay for gemcitabine in plasma was developed and validated to facilitate therapeutic drug monitoring (TDM) by providing an economical, robust method for automated chemistry analyzers...
June 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28487671/improved-mental-acuity-forecasting-with-an-individualized-quantitative-sleep-model
#11
Brent D Winslow, Nam Nguyen, Kimberly E Venta
Sleep impairment significantly alters human brain structure and cognitive function, but available evidence suggests that adults in developed nations are sleeping less. A growing body of research has sought to use sleep to forecast cognitive performance by modeling the relationship between the two, but has generally focused on vigilance rather than other cognitive constructs affected by sleep, such as reaction time, executive function, and working memory. Previous modeling efforts have also utilized subjective, self-reported sleep durations and were restricted to laboratory environments...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28486950/performance-of-mycobacterium-growth-indicator-tube-bactec-960-with-lowenstein-jensen-method-for-diagnosis-of-mycobacterium-tuberculosis-at-ethiopian-national-tuberculosis-reference-laboratory-addis-ababa-ethiopia
#12
Getu Diriba, Abebaw Kebede, Zelalem Yaregal, Muluwork Getahun, Mengistu Tadesse, Abyot Meaza, Zekarias Dagne, Shewki Moga, Jibril Dilebo, Kebebe Gudena, Mulu Hassen, Kassu Desta
BACKGROUND: Bacteriological confirmed active case detection remains the corner stone for diagnosing tuberculosis. Non-radiometric liquid culture system Mycobacterium Growth Indicator Tube with automated interface had been recommended by expert groups in addition to conventional solid culture media such as Lowenstein-Jensen. However in high burden resource limited countries advanced non-radiometric based tuberculosis diagnostic methods such as MGIT 960 is limited. Therefore we have evaluated the performance of MGIT 960 system compared to LJ for recovery of Mycobacterium complex (MTBC) from clinical specimens...
May 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28483925/msstatsqc-longitudinal-system-suitability-monitoring-and-quality-control-for-targeted-proteomic-experiments
#13
Eralp Dogu, Sara Mohammad-Taheri, Susan E Abbatiello, Michael S Bereman, Brendan MacLean, Birgit Schilling, Olga Vitek
Selected Reaction Monitoring (SRM) is a powerful tool for targeted detection and quantification of peptides in complex matrices. An important objective of SRM is to obtain peptide quantifications that are (1) suitable for the purpose of the investigation, and (2) reproducible across laboratories and runs. The first objective is achieved by system suitability tests (SST), which verify that mass spectrometric instrumentation performs as specified. The second objective is achieved by quality control (QC), which provides in-process quality assurance of the sample profile...
May 8, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28483731/evaluation-of-patients-with-cystic-echinococcosis
#14
Emine Türkoğlu, Neşe Demirtürk, Havva Tünay, Murat Akıcı, Gürhan Öz, Didem Baskin Embleton
OBJECTIVE: Cystic echinococcosis (CE) is a globally prevalent zoonotic disease. METHODS: Demographic, clinical, laboratory, and follow-up data of patients between May 2009 and 2015 were retrospectively analyzed by screening data from a hospital automation system. RESULTS: A total of 238 (females, n=139 and males, n=99) patients with a mean age of 40.6±20.58 years were included. Less than half (40.8%) of the patients were living in the countryside...
March 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/28482924/multiplexed-tandem-pcr-for-the-specific-diagnosis-of-gastrointestinal-nematode-infections-in-sheep-an-european-validation-study
#15
Florian Roeber, Alison Morrison, Stijn Casaert, Lee Smith, Edwin Claerebout, Philip Skuce
BACKGROUND: Traditional methods of detecting and identifying gastrointestinal nematode infections in small ruminants, including sheep and goats, are time-consuming and lack in sensitivity and specificity. Recently, we developed an automated multiplexed-tandem (MT)-PCR platform for the diagnosis and identification patent infections with key genera/species of gastrointestinal nematodes of sheep and validated this approach in detailed experiments carried out in Australia. In the present study, we deployed this diagnostic platform in Scotland and Belgium to test samples from naturally infected sheep in these environments and to validate the MT-PCR platform relative to traditional diagnostic methods routinely used by local laboratories...
May 8, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28482830/comparing-national-infectious-disease-surveillance-systems-china-and-the-netherlands
#16
Willemijn L Vlieg, Ewout B Fanoy, Liselotte van Asten, Xiaobo Liu, Jun Yang, Eva Pilot, Paul Bijkerk, Wim van der Hoek, Thomas Krafft, Marianne A van der Sande, Qi-Yong Liu
BACKGROUND: Risk assessment and early warning (RAEW) are essential components of any infectious disease surveillance system. In light of the International Health Regulations (IHR)(2005), this study compares the organisation of RAEW in China and the Netherlands. The respective approaches towards surveillance of arboviral disease and unexplained pneumonia were analysed to gain a better understanding of the RAEW mode of operation. This study may be used to explore options for further strengthening of global collaboration and timely detection and surveillance of infectious disease outbreaks...
May 8, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28480121/alchemy-a-web-2-0-real-time-quality-assurance-platform-for-human-immunodeficiency-virus-hepatitis-c-virus-and-bk-virus-quantitation-assays
#17
Emmanuel Agosto-Arroyo, Gina M Coshatt, Thomas S Winokur, Shuko Harada, Seung L Park
BACKGROUND: The molecular diagnostics laboratory faces the challenge of improving test turnaround time (TAT). Low and consistent TATs are of great clinical and regulatory importance, especially for molecular virology tests. Laboratory information systems (LISs) contain all the data elements necessary to do accurate quality assurance (QA) reporting of TAT and other measures, but these reports are in most cases still performed manually: a time-consuming and error-prone task. The aim of this study was to develop a web-based real-time QA platform that would automate QA reporting in the molecular diagnostics laboratory at our institution, and minimize the time expended in preparing these reports...
2017: Journal of Pathology Informatics
https://www.readbyqxmd.com/read/28479922/laboratory-diagnosis-of-malaria-comparison-of-manual-and-automated-diagnostic-tests
#18
Samina Naz Mukry, Madiha Saud, Gul Sufaida, Kashif Shaikh, Arshi Naz, Tahir Sultan Shamsi
Malaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths. In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by species-specific PCR, microscopy of blood smears, hemoanalyzer Sysmex XE-2100, and rapid test devices (First Response Malaria® and ICT Malaria Combo®). The microscopy detected MP in 126 samples (parasite load/µl 386-53712/µl); 71...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28479850/automated-measurements-of-human-cone-photoreceptor-density-in-healthy-and-degenerative-retina-by-region-based-segmentation
#19
Suguru Miyagawa, Hisashi Fukuyama, Masakazu Hirota, Tatsuo Yamaguchi, Kazuo Kitamura, Takao Endo, Hiroyuki Kanda, Takeshi Morimoto, Takashi Fujikado
PURPOSE: The purpose of this study was to develop an algorithm based on region-based segmentation for automated calculations of human cone photoreceptor density of en face images obtained by an adaptive optics scanning laser ophthalmoscope (AOSLO). SUBJECTS AND METHODS: Cone mosaics of 15 eyes of 15 healthy subjects were photographed by a custom-built AOSLO. The cone density was calculated at 0.5, 1.0, and 1.5 mm temporal from the fovea using a region-based segmentation method (RSM) developed in our laboratory...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28472677/an-automated-system-for-positive-reinforcement-training-of-group-housed-macaque-monkeys-at-breeding-and-research-facilities
#20
Jennifer Tulip, Jonas B Zimmermann, David Farningham, Andrew Jackson
BACKGROUND: Behavioural training through positive reinforcement techniques is a well-recognised refinement to laboratory animal welfare. Behavioural neuroscience research requires subjects to be trained to perform repetitions of specific behaviours for food/fluid reward. Some animals fail to perform at a sufficient level, limiting the amount of data that can be collected and increasing the number of animals required for each study. NEW METHOD: We have implemented automated positive reinforcement training systems (comprising a button press task with variable levels of difficulty using LED cues and a fluid reward) at the breeding facility and research facility, to compare performance across these different settings, to pre-screen animals for selection and refine training protocols...
May 1, 2017: Journal of Neuroscience Methods
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