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https://www.readbyqxmd.com/read/28230179/crowdsourcing-scoring-of-immunohistochemistry-images-evaluating-performance-of-the-crowd-and-an-automated-computational-method
#1
Humayun Irshad, Eun-Yeong Oh, Daniel Schmolze, Liza M Quintana, Laura Collins, Rulla M Tamimi, Andrew H Beck
The assessment of protein expression in immunohistochemistry (IHC) images provides important diagnostic, prognostic and predictive information for guiding cancer diagnosis and therapy. Manual scoring of IHC images represents a logistical challenge, as the process is labor intensive and time consuming. Since the last decade, computational methods have been developed to enable the application of quantitative methods for the analysis and interpretation of protein expression in IHC images. These methods have not yet replaced manual scoring for the assessment of IHC in the majority of diagnostic laboratories and in many large-scale research studies...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229894/mobile-stroke-units-for-prehospital-thrombolysis-triage-and-beyond-benefits-and-challenges
#2
REVIEW
Klaus Fassbender, James C Grotta, Silke Walter, Iris Q Grunwald, Andreas Ragoschke-Schumm, Jeffrey L Saver
In acute stroke management, time is brain. Bringing swift treatment to the patient, instead of the conventional approach of awaiting the patient's arrival at the hospital for treatment, is a potential strategy to improve clinical outcomes after stroke. This strategy is based on the use of an ambulance (mobile stroke unit) equipped with an imaging system, a point-of-care laboratory, a telemedicine connection to the hospital, and appropriate medication. Studies of prehospital stroke treatment consistently report a reduction in delays before thrombolysis and cause-based triage in regard to the appropriate target hospital (eg, primary vs comprehensive stroke centre)...
March 2017: Lancet Neurology
https://www.readbyqxmd.com/read/28227680/a-mobile-platform-for-automated-screening-of-asthma-and-chronic-obstructive-pulmonary-disease
#3
Daniel B Chamberlain, Rahul Kodgule, Richard Ribon Fletcher, Daniel B Chamberlain, Rahul Kodgule, Richard Ribon Fletcher, Daniel B Chamberlain, Richard Ribon Fletcher, Rahul Kodgule
Chronic Obstructive Pulmonary Disease (COPD) and asthma each represent a large proportion of the global disease burden; COPD is the third leading cause of death worldwide and asthma is one of the most prevalent chronic diseases, afflicting over 300 million people. Much of this burden is concentrated in the developing world, where patients lack access to physicians trained in the diagnosis of pulmonary disease. As a result, these patients experience high rates of underdiagnosis and misdiagnosis. To address this need, we present a mobile platform capable of screening for Asthma and COPD...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227671/imquant-an-image-based-fluorescence-reader-for-quantitative-lateral-flow-immunoassays
#4
Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam, Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam, Jayaraj Joseph, Kaushik Basak Chowdhury
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free, automated reader for accurate quantification of immunoassays. Here, we present ImQuant, an image based FIA reader with integrated monochromatic excitation source, optical filtering and real time image capture, assisted with an intuitive graphical user interface...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227197/identifying-individual-sleep-apnea-hypoapnea-epochs-using-smartphone-based-pulse-oximetry
#5
Ainara Garde, Parastoo Dekhordi, J Mark Ansermino, Guy A Dumont, Ainara Garde, Parastoo Dekhordi, J Mark Ansermino, Guy A Dumont, J Mark Ansermino, Parastoo Dekhordi, Guy A Dumont, Ainara Garde
Sleep apnea, characterized by frequent pauses in breathing during sleep, poses a serious threat to the healthy growth and development of children. Polysomnography (PSG), the gold standard for sleep apnea diagnosis, is resource intensive and confined to sleep laboratories, thus reducing its accessibility. Pulse oximetry alone, providing blood oxygen saturation (SpO2) and blood volume changes in tissue (PPG), has the potential to identify children with sleep apnea. Thus, we aim to develop a tool for at-home sleep apnea screening that provides a detailed and automated 30 sec epoch-by-epoch sleep apnea analysis...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227178/a-smartphone-application-suite-for-assessing-mobility
#6
Priyanka Madhushri, Armen A Dzhagaryan, Emil Jovanov, Aleksandar Milenkovic, Priyanka Madhushri, Armen A Dzhagaryan, Emil Jovanov, Aleksandar Milenkovic, Armen A Dzhagaryan, Priyanka Madhushri, Emil Jovanov, Aleksandar Milenkovic
Modern smartphones integrate a growing number of inertial and environmental sensors that can enable the development of new mobile health applications. In this paper we introduce a suite of smartphone applications for assessing mobility in elderly population. The suite currently includes applications that automate and quantify the following standardized medical tests for assessing mobility: Timed-Up-and-Go (TUG), 30 Seconds Chair Stand Test (30SCS), and a 4-stage Balance Test (4SBT). For each smartphone application we describe its functionality and a list of parameters extracted by processing signals from smartphone's inertial sensors...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226582/towards-holistic-free-living-assessment-in-parkinson-s-disease-unification-of-gait-and-fall-algorithms-with-a-single-accelerometer
#7
Alan Godfrey, Alan Bourke, Silvia Del Din, Rosie Morris, Aodhan Hickey, Jorunn L Helbostad, Lynn Rochester, Alan Godfrey, Alan Bourke, Silvia Del Din, Rosie Morris, Aodhan Hickey, Jorunn L Helbostad, Lynn Rochester, Silvia Del Din, Rosie Morris, Alan Godfrey, Lynn Rochester, Jorunn L Helbostad, Aodhan Hickey, Alan Bourke
Technological developments have seen the miniaturization of sensors, small enough to be embedded in wearable devices facilitating unobtrusive and longitudinal monitoring in free-living environments. Concurrently, the advances in algorithms have been ad-hoc and fragmented. To advance the mainstream use of wearable technology and improved functionality of algorithms all methodologies must be unified and robustly tested within controlled and free-living conditions. Here we present and unify a (i) gait segmentation and analysis algorithm and (ii) a fall detection algorithm...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226272/automated-processing-extraction-and-detection-of-herpes-simplex-virus-types-1-and-2-a-comparative-evaluation-of-three-commercial-platforms-using-clinical-specimens
#8
Matthew J Binnicker, Mark J Espy, Brian Duresko, Cole Irish, Jay Mandrekar
BACKGROUND: Recently, automated platforms have been developed that can perform processing, extraction and testing for herpes simplex virus (HSV) nucleic acid on a single instrument. OBJECTIVES: In this study, we compared three commercially-available systems; Aptima(®)/Panther (Hologic, San Diego, CA), ARIES(®) (Luminex Corporation, Austin, TX), and cobas(®) 4800 (Roche Molecular Systems Inc, Pleasanton, CA) for the qualitative detection of HSV-1/2 in clinical samples...
February 14, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28224770/performance-of-an-automated-fluorescence-antinuclear-antibody-image-analyzer
#9
In Young Yoo, Jong Won Oh, Hoon Suk Cha, Eun Mi Koh, Eun Suk Kang
BACKGROUND: The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing. We evaluated the performance of EUROPattern Suite (Euroimmun AG, Germany), an automated FANA image analyzer, with regard to ANA detection and pattern recognition compared with conventional manual interpretation using the fluorescence microscopic IIF assay...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224056/evaluation-and-comparison-of-fta-card-and-ctab-dna-extraction-methods-for-non-agricultural-taxa
#10
Chloe S Siegel, Florence O Stevenson, Elizabeth A Zimmer
PREMISE OF THE STUDY: An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS: DNA was extracted using FTA cards according to the manufacturer's protocol. In parallel, CTAB-based extractions were done using the automated AutoGen DNA isolation system...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28221923/matrix-specific-method-validation-of-an-automated-most-probable-number-system-for-use-in-measuring-bacteriological-quality-of-grade-a-milk-products
#11
Samantha Lindemann, Matthew Kmet, Ravinder Reddy, Steffen Uhlig
The U.S. Food and Drug Administration (FDA) oversees a long-standing cooperative federal and state milk sanitation program that uses the grade "A" Pasteurized Milk Ordinance standards to maintain the safety of grade "A" milk sold in the United States. The Pasteurized Milk Ordinance requires that grade "A" milk samples be tested using validated total aerobic bacterial and coliform count methods. The objective of this project was to conduct an interlaboratory method validation study to compare performance of a film plate method with an automated most-probable-number method for total aerobic bacterial and coliform counts, using statistical approaches from international data standards...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28217778/a-critical-insight-into-the-development-pipeline-of-microfluidic-immunoassay-devices-for-the-sensitive-quantitation-of-protein-biomarkers-at-the-point-of-care
#12
REVIEW
Ana I Barbosa, Nuno M Reis
The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories...
February 20, 2017: Analyst
https://www.readbyqxmd.com/read/28217081/blinker-automated-extraction-of-ocular-indices-from-eeg-enabling-large-scale-analysis
#13
Kelly Kleifges, Nima Bigdely-Shamlo, Scott E Kerick, Kay A Robbins
Electroencephalography (EEG) offers a platform for studying the relationships between behavioral measures, such as blink rate and duration, with neural correlates of fatigue and attention, such as theta and alpha band power. Further, the existence of EEG studies covering a variety of subjects and tasks provides opportunities for the community to better characterize variability of these measures across tasks and subjects. We have implemented an automated pipeline (BLINKER) for extracting ocular indices such as blink rate, blink duration, and blink velocity-amplitude ratios from EEG channels, EOG channels, and/or independent components (ICs)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28214393/flow-cytometric-bacterial-cell-counts-challenge-conventional-heterotrophic-plate-counts-for-routine-microbiological-drinking-water-monitoring
#14
REVIEW
S Van Nevel, S Koetzsch, C R Proctor, M D Besmer, E I Prest, J S Vrouwenvelder, A Knezev, N Boon, F Hammes
Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies...
February 8, 2017: Water Research
https://www.readbyqxmd.com/read/28213280/magnetic-lateral-flow-immunoassay-test-strip-development-considerations-for-proof-of-concept-evaluation
#15
R Connolly, R O' Kennedy
Lateral flow immunoassays (LFIA) have grown to become the predominant test device format for the diagnostics and point-of-care industries. The demand for robust and reproducible LFIAs has been facilitated through scale-up production methods using specialized and automated instruments. However, the feasibility of a LFIA device can still be evaluated in a small-scale laboratory setting through controlled manual preparation methods. The advent of super-paramagnetic (SPMP) labels for use in lateral flow has heralded the possibility of highly sensitive and stable LFIAs...
February 14, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28212711/advances-in-clinical-mass-spectrometry
#16
D French
Although mass spectrometry has been used clinically for decades, the advent of immunoassay technology moved the clinical laboratory to more labor saving automated platforms requiring little if any sample preparation. It became clear, however, that immunoassays lacked sufficient sensitivity and specificity necessary for measurement of certain analytes or for measurement of analytes in specific patient populations. This limitation prompted clinical laboratories to revisit mass spectrometry which could additionally be used to develop assays for which there was no commercial source...
2017: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/28193030/dynamic-cluster-analysis-an-unbiased-method-for-identifying-a-2-element-containing-compounds-in-liquid-chromatographic-high-resolution-time-of-flight-mass-spectrometric-data
#17
Aaron John Christian Andersen, Per Juel Hansen, Kevin Jørgensen, Kristian Fog Nielsen
Dynamic cluster analysis (DCA) is an automated, unbiased technique which can identify Cl, Br, S, and other A + 2 element containing metabolites in liquid chromatographic high-resolution mass spectrometric data. DCA is based on three features, primarily the previously unutilized A + 1 to A + 2 isotope cluster spacing which is a strong classifier in itself but improved with the addition of the monoisotopic mass, and the well-known A:A+2 intensity ratio. Utilizing only the A + 1 to A + 2 isotope cluster spacing and the monoisotopic mass it was possible to filter a chromatogram for metabolites which contain Cl, Br, and S...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28189099/miniaturising-acute-toxicity-and-feeding-rate-measurements-in-daphnia-magna
#18
Konstantinos Grintzalis, Wenkui Dai, Konstantinos Panagiotidis, Alexia Belavgeni, Mark R Viant
Phenotypic markers of animal health form an essential component of regulatory toxicology. Immobilisation of neonate water fleas - Daphnia magna - as a surrogate measure of their mortality following exposure to a chemical for 24-48h forms the basis of the internationally utilised OECD acute toxicity test 202. A second important marker of animal physiology and health is feeding rate, which in Daphnia is determined by measuring the algae feeding rate. Given the widespread use of OECD test 202 for acute toxicity as well as the quantification of feeding rate in toxicological studies of daphniids, significant benefits could result from miniaturising this assay...
February 8, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28188739/comparison-of-automated-devices-ux-2000-and-sedimax-autionmax-for-urine-samples-screening-a-multicenter-spanish-study
#19
Catalina Sánchez-Mora, Delia Acevedo, Maria Amelia Porres, Ana María Chaqués, Javier Zapardiel, Aurelia Gallego-Cabrera, Jose María López, Jose María Maesa
OBJECTIVES: In this study we aim to compare UX2000 (Sysmex Corp, Japan) and SediMAX/AutionMax (Arkray Factory Inc., Japan), totally automatized analyzers, against Fuchs-Rosenthal counting chamber, the gold standard technique for sediment analysis. DESIGN AND METHODS: Urine samples of 1454 patients from three Spanish hospitals were assessed for red and white blood cells (RBC; WBC) using three different techniques: flow cytometry, image-based method and Fuchs-Rosenthal counting chamber...
February 7, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28188528/lumier-a-discovery-tool-for-mammalian-protein-interaction-networks
#20
Miriam Barrios-Rodiles, Jonathan D Ellis, Benjamin J Blencowe, Jeffrey L Wrana
Protein-protein interactions (PPIs) play an essential role in all biological processes. In vivo, PPIs occur dynamically and depend on extracellular cues. To discover novel protein-protein interactions in mammalian cells, we developed a high-throughput automated technology called LUMIER (LUminescence-based Mammalian IntERactome). In this approach, we co-express a Luciferase (LUC)-tagged fusion protein along with a Flag-tagged protein in an efficiently transfectable cell line such as HEK-293T cells. The interaction between the two proteins is determined by co-immunoprecipitation using an anti-Flag antibody, and the presence of the LUC-tagged interactor in the complex is subsequently detected via its luciferase activity...
2017: Methods in Molecular Biology
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