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Digital Pathology

David W Shofler, Bryon McKenna, Johnny Huang, Robert A Christman
BACKGROUND: Tailor's bunion, or bunionette, deformity is a common condition of the lateral forefoot. To aid in assessing the deformity, radiographic angular measurements are frequently used. The objective of this study was to determine the reliability and reproducibility of these angular measurements. METHODS: Thirty unique weightbearing dorsoplantar digital radiographs of pathologic feet were compiled. For these 30 radiographs and for ten repeated radiographs, six practicing clinicians measured the following angles: the fourth to fifth intermetatarsal angle, the lateral deviation angle, and the fifth metatarsophalangeal angle...
May 2018: Journal of the American Podiatric Medical Association
Alexi Baidoshvili, Anca Bucur, Jasper van Leeuwen, Jeroen van der Laak, Philip Kluin, Paul J van Diest
BACKGROUND: The benefits of digital pathology for workflow improvement and thereby cost savings in pathology, at least partly outweighing investment costs, are increasingly recognized. Successful implementations in a variety of scenarios start to demonstrate cost benefits of digital pathology for both research and routine diagnostics, contributing to a sound business case encouraging further adoption. To further support new adopters, there is still a need for detailed assessment of the impact this technology has on the relevant pathology workflows with emphasis on time saving...
June 20, 2018: Histopathology
Shigeomi Yokoya, Aiko Tamura, Akihiko Hino, Masamichi Bamba, Toshikazu Kato, Hidesato Takezawa, Yukihiro Goto, Hideki Oka, Akihiro Fujii
BACKGROUND: Kawasaki disease (KD) is an acute systemic vasculitis that primarily affects the coronary artery, but does not commonly affect the carotid artery. Cerebral infarction (CI) with internal carotid artery stenosis (ICS) in patients with KD had never been reported. We report a patient with CI as a remote phase complication of KD. CASE PRESENTATION: A 32-year-old man presented with impaired consciousness. Magnetic resonance imaging scan (MRI) and digital subtraction angiogram (DSA) confirmed CI and ICS...
June 16, 2018: World Neurosurgery
Henrik Helin, Teemu Tolonen, Onni Ylinen, Petteri Tolonen, Juha Näpänkangas, Jorma Isola
Background: Whole slide images (WSIs, digitized histopathology glass slides) are large data files whose long-term storage remains a significant cost for pathology departments. Currently used WSI formats are based on lossy image compression alogrithms, either using JPEG or its more efficient successor JPEG 2000. While the advantages of the JPEG 2000 algorithm (JP2) are commonly recognized, its compression parameters have not been fully optimized for pathology WSIs. Methods: We defined an optimized parametrization for JPEG 2000 image compression, designated JP2-WSI, to be used specifically with histopathological WSIs...
2018: Journal of Pathology Informatics
Anil Reddy Konduru, Balasaheb R Yelikar, K V Sathyashree, Ankur Kumar
Background: Open source technologies and mobile innovations have radically changed the way people interact with technology. These innovations and advancements have been used across various disciplines and already have a significant impact. Microscopy, with focus on visually appealing contrasting colors for better appreciation of morphology, forms the core of the disciplines such as Pathology, microbiology, and anatomy. Here, learning happens with the aid of multi-head microscopes and digital camera systems for teaching larger groups and in organizing interactive sessions for students or faculty of other departments...
2018: Journal of Pathology Informatics
Sean D McGarry, Sarah L Hurrell, Kenneth A Iczkowski, William Hall, Amy L Kaczmarowski, Anjishnu Banerjee, Tucker Keuter, Kenneth Jacobsohn, John D Bukowy, Marja T Nevalainen, Mark D Hohenwalter, William A See, Peter S LaViolette
PURPOSE: This study aims to combine multiparametric magnetic resonance imaging (MRI) and digitized pathology with machine learning to generate predictive maps of histologic features for prostate cancer localization. METHODS AND MATERIALS: Thirty-nine patients underwent MRI prior to prostatectomy. After surgery, tissue was sliced according to MRI orientation using patient-specific 3-dimensionally printed slicing jigs. Whole-mount sections were annotated by our pathologist and digitally contoured to differentiate the lumen and epithelium...
April 24, 2018: International Journal of Radiation Oncology, Biology, Physics
N Morchiladze, B Tkeshelashvili, T Gagua, D Gagua
The isolated hypothyroxinemia of pregnancy (IHP) has gained specific attention in the specialized literature during the recent years as the possible factor impeding the intellectual development of fetus and increasing the risk of complications related with pregnancy, delivery and perinatal period. Aim of the study was to define the importance of isolated hypothyroxinemia in the development of obstetric and somatic pathologies in outpatient population of pregnant females. The study of prospective design was performed at the base of "David Gagua Clinic" Ltd...
May 2018: Georgian Medical News
A Carrieroa, A F Pereirab, A J Wilson, S Castagno, B Javaheri, A A Pitsillides, M Marenzana, S J Shefelbine
Bone is a dynamic tissue and adapts its architecture in response to biological and mechanical factors. Here we investigate how cortical bone formation is spatially controlled by the local mechanical environment in the murine tibia axial loading model (C57BL/6). We obtained 3D locations of new bone formation by performing 'slice and view' 3D fluorochrome mapping of the entire bone and compared these sites with the regions of high fluid velocity or strain energy density estimated using a finite element model, validated with ex-vivo bone surface strain map acquired ex-vivo using digital image correlation...
June 2018: Bone Reports
Wanxia Gai, Lu Ji, W K Jacky Lam, Kun Sun, Peiyong Jiang, Anthony W H Chan, John Wong, Paul B S Lai, Simon S M Ng, Brigette B Y Ma, Grace L H Wong, Vincent W S Wong, Henry L Y Chan, Rossa W K Chiu, Y M Dennis Lo, K C Allen Chan
BACKGROUND: Measurement of DNA derived from different tissues in the circulating DNA pool can provide important information regarding the presence of many pathological conditions. However, existing methods involving genome-wide bisulfite sequencing are relatively expensive and may present challenges for large-scale analysis. METHODS: Through identifying differentially methylated regions in the liver and colon compared with other tissues, we identified 2 markers and developed corresponding droplet digital PCR assays...
June 14, 2018: Clinical Chemistry
Y N Wang, H T Wang
Objective: To observe the process of nasal mucosa injury and repair induced by nasal decongestants in guinea pigs Methods: Sixty-five male guinea pigs were randomly divided into 4groups by digital random method.The guinea pigs in Group A (20 guinea pigs)were treated with 2 sprays of 0.1% Naphazoline 6 times a day for 2 weeks; Group B (20 guinea pigs)with 2 drops of 1% Ephedrine 6 times a day for 2 weeks; Group C(20 guinea pigs) with 2 sprays of Naphazolin hydrochloride and Chlorphenamine Maleate Nasal Spray 8 times a day for 2 weeks...
June 7, 2018: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Sriram Somanchi, Daniel B Neill, Anil V Parwani
Advances in medical imaging technology have created opportunities for computer-aided diagnostic tools to assist human practitioners in identifying relevant patterns in massive, multiscale digital pathology slides. This work presents Hierarchical Linear Time Subset Scanning, a novel statistical method for pattern detection. Hierarchical Linear Time Subset Scanning exploits the hierarchical structure inherent in data produced through virtual microscopy in order to accurately and quickly identify regions of interest for pathologists to review...
June 13, 2018: Statistics in Medicine
Koji Kasanuki, Keith A Josephs, Tanis J Ferman, Melissa E Murray, Shunsuke Koga, Takuya Konno, Nobutaka Sakae, Adam Parks, Ryan J Uitti, Jay A Van Gerpen, Neill R Graff-Radford, Zbigniew K Wszolek, Dennis W Dickson
OBJECTIVE: To describe clinical and pathologic characteristics of diffuse Lewy body disease (DLBD) manifesting as corticobasal syndrome (CBS). METHODS: In 523 autopsy-confirmed cases of DLBD, we identified 11 patients diagnosed with CBS. For comparison, we studied 22 DLBD brains with antemortem presentation of dementia with Lewy bodies (DLB). Given previous studies suggesting the importance of pathology in peri-Rolandic cortices in CBS, we used digital pathology to count Lewy bodies and to quantify intracytoplasmic and neuritic α-synuclein and phospho-tau burden in the motor cortex...
June 13, 2018: Neurology
Cleo-Aron Weis, Inmaculada B Aban, Garry Cutter, Henry J Kaminski, Christoph Scharff, Benedict W Grießmann, Maria Deligianni, Klaus Kayser, Gil I Wolfe, Philipp Ströbel, Alexander Marx
The thymectomy specimens from the "thymectomy trial in non-thymomatous myasthenia gravis patients receiving prednisone therapy" (MGTX) underwent rigid and comprehensive work-up, which permits analysis of the spatial distribution of histological and immunohistological features. This analysis revealed strong intra- and inter-case variability. While many histological features (e.g. median percent fat content among different specimens) can easily be correlated with clinical parameters, intra-case spatial variability of histological features has yet defied quantification and statistical evaluation...
2018: PloS One
L Huo, M-J Chen, C Yang, S-Y Zhang, J-S Zheng, Y Chen
We have introduced an effective treatment for mandibular condylar osteochondroma with a digital cutting guide and endoscopically-assisted vertical ramus osteotomy (VRO). Eleven patients with unilateral condylar osteochondroma, who did not require orthognathic surgery or had less than 3mm deviation of the chin and a stable occlusion, were treated during the period April 2013-January 2017 with a digital cutting guide and endoscopically-assisted VRO. Clinical data collected included the occlusion, facial contour, and maximum mouth opening (MMO)...
June 9, 2018: British Journal of Oral & Maxillofacial Surgery
Sophie Goettmann, Isabelle Moulonguet, Inès Zaraa
BACKGROUND: Nail unit melanoma (NUM) is a rare melanoma variant, usually associated with a poor prognosis because of a delayed diagnosis. Few data are available concerning the management and long-term outcome of in situ NUM. OBJECTIVE: To use a large cohort to provide comprehensive patient data and long-term follow-up information. This will serve to investigate distinctive epidemiological, clinical, and histological features of in situ NUM. to report treatment modalities, assess conservative surgery and evaluate its long-term safety...
June 12, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
Alexi Baidoshvili, Nikolas Stathonikos, Gerard Freling, Jos Bart, Nils 't Hart, Jeroen van der Laak, Jan Doff, Bert van der Vegt, Philip Kluin, Paul J van Diest
BACKGROUND: Most validation studies on digital pathology diagnostics have been performed in single institutes. Since rapid consultation of extramural experts is one of the most important use cases for digital pathology laboratory networks, the aim of this study was to validate a whole slide image-based teleconsultation network between three independent laboratories. METHODS: Each laboratory contributed 30 biopsies and / or excisions, adding up to 90 specimens (776 slides) of varying difficulty and covering a wide variety of organs and subspecialties...
June 12, 2018: Histopathology
Jeneen Panezai, Ambereen Ghaffar, Mohammad Altamash, Per-Erik Engström, Anders Larsson
BACKGROUND: Periodontal disease (PD) and rheumatoid arthritis (RA) are bone pathologies mediated through immuno-inflammatory mechanisms. The aim of this study was to investigate the serum markers osteopontin (OPN), tumor necrosis factor receptors 1 (TNFR1) and 2 (TNFR2) receptor activator of nuclear factor-kappa B ligand (RANKL) and RANKL/ osteoprotegerin (OPG) ratio and compare them in PD and RA groups. MATERIALS & METHODS: RA (with PD = 19 and without PD = 19), PD (n = 38) and 14 healthy subjects underwent bleeding on probing (BOP) and probing pocket depth (PPD) measurement...
2018: PloS One
Rüdiger Schulz-Wendtland, Caroline Preuss, Peter A Fasching, Christian R Loehberg, Michael P Lux, Julius Emons, Matthias W Beckmann, Michael Uder, Markus Mueller-Schimpfle
Introduction: For decades, conventional galactography was the only imaging technique capable of showing the mammary ducts. Today, diagnosis is based on a multimodal concept which combines high-resolution ultrasound with magnetic resonance (MR) mammography and ductoscopy/galactoscopy and has a sensitivity and specificity of up to 95%. This study used tomosynthesis in galactography for the first time and compared the synthetic digital 2D full-field mammograms generated with this technique with the images created using the established method of ductal sonography...
May 2018: Geburtshilfe und Frauenheilkunde
Zeran Li, Jorge L Del-Aguila, Umber Dube, John Budde, Rita Martinez, Kathleen Black, Qingli Xiao, Nigel J Cairns, Joseph D Dougherty, Jin-Moo Lee, John C Morris, Randall J Bateman, Celeste M Karch, Carlos Cruchaga, Oscar Harari
BACKGROUND: Alzheimer's disease (AD) is characterized by neuronal loss and astrocytosis in the cerebral cortex. However, the specific effects that pathological mutations and coding variants associated with AD have on the cellular composition of the brain are often ignored. METHODS: We developed and optimized a cell-type-specific expression reference panel and employed digital deconvolution methods to determine brain cellular distribution in three independent transcriptomic studies...
June 8, 2018: Genome Medicine
Donatella Lucchetti, Andrea Fattorossi, Alessandro Sgambato
Background The possibility to study solid tumors through the analysis of extracellular vesicles in biological fluids is one of the most exciting and rapidly advancing field in cancer research. The extracellular vesicles are tiny sacs released in both physiological and pathological conditions and could be used to monitor the evolution of several pathological states, including neoplastic diseases. Indeed, these vesicles carry biological informations and can affect the behavior of recipient cells by transferring proteins, DNA, RNA and microRNA...
June 7, 2018: Biotechnology Journal
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