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Digital Pathology

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https://www.readbyqxmd.com/read/29228211/asymmetry-of-post-mortem-neuropathology-in-behavioural-variant-frontotemporal-dementia
#1
David J Irwin, Corey T McMillan, Sharon X Xie, Katya Rascovsky, Vivianna M Van Deerlin, H Branch Coslett, Roy Hamilton, Geoffrey Aguirre, Edward B Lee, Virginia M Y Lee, John Q Trojanowski, Murray Grossman
Antemortem behavioural and anatomic abnormalities have largely been associated with right hemisphere disease in behavioural-variant frontotemporal dementia, but post-mortem neuropathological examination of bilateral hemispheres remains to be defined. Here we measured the severity of post-mortem pathology in both grey and white matter using a validated digital image analysis method in four cortical regions sampled from each hemisphere in 26 patients with behavioural-variant frontotemporal dementia, including those with frontotemporal degeneration (i...
December 8, 2017: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/29222437/digital-pcr-for-gene-expression-impact-from-inherent-tissue-rna-degradation
#2
Melanie J Millier, Lisa K Stamp, Paul A Hessian
Subtle molecular differences indicate the heterogeneity present in a number of disease settings. Digital-PCR (dPCR) platforms achieve the necessary levels of sensitivity and accuracy over standard quantitative RT-PCR (qPCR) that promote their use for such situations, detecting low abundance transcript and subtle changes from gene expression. An underlying requisite is good quality RNA, principally dictated by appropriate tissue handling and RNA extraction. Here we consider the application of dPCR to measures of gene expression in pathological tissues with inherent necrosis, focusing on rheumatoid subcutaneous nodules...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29220095/digital-image-analysis-of-ki67-in-hot-spots-is-superior-to-both-manual-ki67-and-mitotic-counts-in-breast-cancer
#3
Gustav Stålhammar, Stephanie Robertson, Lena Wedlund, Michael Lippert, Mattias Rantalainen, Jonas Bergh, Johan Hartman
AIMS: During pathological examination of breast tumours, proliferative activity is routinely evaluated by a count of mitoses. Adding immunohistochemical stains of Ki67 provides extra prognostic and predictive information. However, currently used methods for these evaluations suffer from imperfect reproducibility. It is still unclear whether analysis of Ki67 should be performed in hot spots, in the tumour periphery or as an average of the whole tumour section. In this paper, we compare the clinical relevance of mitoses, Ki67 and Phosphohistone H3 in two cohorts of primary breast cancer specimens (total n=294)...
December 8, 2017: Histopathology
https://www.readbyqxmd.com/read/29219668/multispectral-imaging-reveals-hyper-active-tgf-%C3%AE-signaling-in-colorectal-cancer
#4
Lei Yang, Zheng Liu, Jinjing Tan, Hongwei Dong, Xiaojing Zhang
Advances in multiplex immunohistochemistry (IHC) techniques and digital pathology platforms allow quantification of multiple proteins at same tissue section and produce continuous data. TGF-β signaling plays crucial and complex roles in colorectal cancer (CRC). We here aimed to investigate clinical pathological relevant of proteins involved in TGF-β signaling at CRC tissues. Multiplex fluorescent IHC was used to quantitative analysis. The levels of eight proteins (TGF-β1, TGFBRI, TGFBRII, SMAD4, SMAD2/3, p-SMAD2/3, SMAD1/5/9, and p-SMAD1/5/9) were determined in TMA sections...
December 8, 2017: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29216704/-prevalence-of-x-ray-signs-of-femoroacetabular-impingement-in-mexican-population
#5
R Gutiérrez-Ramos, S A Ávalos-Calderón, L A Bahena-Peniche
BACKGROUND: Femoroacetabular impingement (FAI) is a suggested diagnosis for pain in the hip in the absence of other apparent causes. It is thought to be a cause of progressive arthrosis of the hip. Our objective was to determine the prevalence of radiographic data of FAI in the adult population without hip pathology over a period of six months. MATERIAL AND METHODS: Analytical, observational, multicentric, ambispective and transverse study based on digital X-rays analysis...
May 2017: Acta Ortopédica Mexicana
https://www.readbyqxmd.com/read/29211355/tumor-marker-%C3%AE-fetoprotein-receptor-does-not-discriminate-between-benign-prostatic-disease-and-prostate-cancer
#6
Tomaž Smrkolj, Borut Gubina, Jure Bizjak, Kristina Kumer, Teja Fabjan, Joško Osredkar
BACKGROUND: The α-fetoprotein receptor (RECAF) is a proposed novel tumor marker for detecting several different types of tumors, including prostate cancer (PCa). OBJECTIVES: The aim of the study was to evaluate RECAF in discriminating benign prostatic conditions from PCa and to compare it with prostate-specific antigen (PSA). MATERIAL AND METHODS: A total of 64 patients with elevated serum PSA levels and/or abnormal digital rectal examination of the prostate referred to a tertiary center for transrectal ultrasound (TRUS) biopsy of the prostate were prospectively enrolled in the study from January 2009 to April 2010...
October 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29209735/atypical-lipomatous-tumor-of-the-hand-with-transformation-to-dedifferentiated-liposarcoma-a-case-report
#7
Daniel C Ramirez, Sinchun Hwang, Edward A Athanasian, Lu Wang, Meera Hameed
Atypical lipomatous tumor/well-differentiated liposarcoma is the most common sarcoma of soft tissue in adults. We describe the clinical, radiologic, and pathologic features of an atypical lipomatous tumor arising within the soft tissue of the left hand of a 68-year-old female that underwent transformation to dedifferentiated liposarcoma and eventually metastasized. At initial presentation, imaging demonstrated an extensively calcified fatty soft tissue mass with displacement of the digits. Following biopsy and staged debulking, the patient subsequently developed local recurrence, dedifferentiation, and widespread metastases to the lungs, pancreas, bone, and soft tissues...
December 5, 2017: Skeletal Radiology
https://www.readbyqxmd.com/read/29207599/automated-histological-classification-of-whole-slide-images-of-colorectal-biopsy-specimens
#8
Hiroshi Yoshida, Yoshiko Yamashita, Taichi Shimazu, Eric Cosatto, Tomoharu Kiyuna, Hirokazu Taniguchi, Shigeki Sekine, Atsushi Ochiai
Background: An automated image analysis system, e-Pathologist, was developed to improve the quality of colorectal biopsy diagnostics in routine pathology practice. Objective: The aim of the study was to evaluate the classification accuracy of the e-Pathologist image analysis software in the setting of routine pathology practice in two institutions. Materials and methods: In total, 1328 colorectal tissue specimens were consecutively obtained from two hospitals (1077 tissues from Tokyo hospital, and 251 tissues from East hospital) and the stained specimen slides were anonymized and digitized...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29203879/qupath-open-source-software-for-digital-pathology-image-analysis
#9
Peter Bankhead, Maurice B Loughrey, José A Fernández, Yvonne Dombrowski, Darragh G McArt, Philip D Dunne, Stephen McQuaid, Ronan T Gray, Liam J Murray, Helen G Coleman, Jacqueline A James, Manuel Salto-Tellez, Peter W Hamilton
QuPath is new bioimage analysis software designed to meet the growing need for a user-friendly, extensible, open-source solution for digital pathology and whole slide image analysis. In addition to offering a comprehensive panel of tumor identification and high-throughput biomarker evaluation tools, QuPath provides researchers with powerful batch-processing and scripting functionality, and an extensible platform with which to develop and share new algorithms to analyze complex tissue images. Furthermore, QuPath's flexible design makes it suitable for a wide range of additional image analysis applications across biomedical research...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29203739/4-and-6-interleukin-s-action-in-the-pathogenesis-of-periodontitis-gingivitis-and-dental-alveolitis
#10
Alla I Berezniakova, Valentуna F Cheremisina
INTRODUCTION: The paper presents the results of studying the role of interleukins 4 and 6 in the pathogenesis of periodontal tissue diseases, specifically, in periodontitis, gingivitis and alveolitis. THE AIM: To study the nature of participation of IL-4 and IL-6 in the mechanisms of development of periodontitis, gingivitis and alveolitis. MATERIALS AND METHODS: Studies were carried out on 80 nonlinear male rats with a body weight of 200.0 to 220...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29203379/arthroscopic-reconstruction-of-the-ligamentum-teres-a-guide-to-safe-tunnel-placement
#11
Alex W Brady, Jorge Chahla, Renato Locks, Jacob D Mikula, Erik L Slette, Robert F LaPrade, Marc J Philippon
PURPOSE: To provide a quantitative guide to tunnel placement concurrently through the femur and acetabulum during a ligamentum teres reconstruction, minimizing the risk of injury to the obturator neurovascular bundle. METHODS: Nine human cadaveric pelvises, complete with femurs (mean age, 59.6 years; age range, 47-65 years), were studied. Before dissection, a 3-dimensional coordinate-measuring device was used to record the neutral orientation of the femur in the acetabulum...
December 1, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29193851/survival-of-corneal-nerve-sheath-structures-in-organ-cultured-donor-corneas
#12
Virinder K Dhillon, Mohamed S Elalfy, Marco Messina, Mouhamed Al-Aqaba, Harminder S Dua
PURPOSE: To study the morphology of human corneal nerves in eye bank organ-cultured corneas and in corneal grafts post-transplantation. METHODS: Thirty-seven organ-cultured corneas were divided into: Group-A, anterior 300-400 μm of 20 corneas used for Descemets stripping endothelial keratoplasty, and Group-B, 17 full-thickness corneas unsuitable for transplantation. Corneas whole mounts were stained for nerves using acetylcholinesterase technique and examined by NanoZoomer digital pathology microscope...
November 29, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/29191678/comparison-of-digital-color-fundus-imaging-and-fluorescein-angiographic-findings-for-the-early-detection-of-diabetic-retinopathy-in-young-type-1-diabetic-patients
#13
Z Kapsala, A Anastasakis, D Mamoulakis, I Maniadaki, M Tsilimbaris
PURPOSE: To compare the findings from digital 7-field color fundus (CF) photography and fundus fluorescein angiography (FFA) in young patients with diabetes mellitus (DM) type 1 without known diabetic retinopathy. METHODS: In this prospective, observational cohort study, 54 type 1 diabetic patients were recruited. Participants had been diagnosed with diabetes mellitus (DM) for at least 6 years, had Best Corrected Visual Acuity of 20/25 or better and did not have any known retinal pathology...
November 27, 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/29182386/in-vivo-evaluation-of-effects-of-sedation-on-results-of-acoustoelastography-of-the-superficial-digital-flexor-tendons-in-clinically-normal-horses
#14
Diego De Gasperi, Samantha L Dzierzak, Peter Muir, Ray Vanderby, Sabrina H Brounts
OBJECTIVE To assess the effects of sedation on results of acoustoelastography of the superficial digital flexor tendons (SDFTs) in clinically normal horses. ANIMALS 27 clinically normal horses. PROCEDURES For each horse, the pathology index (PI) for the SDFT of each thoracic limb was determined by use of acoustoelastography at 4 locations (5, 10, 15, and 20 cm distal to the accessory carpal bone). Horses were evaluated before and after they were sedated with a combination of detomidine hydrochloride (0.01 mg/kg, IV) and butorphanol tartrate (0...
December 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29175265/digital-image-analysis-in-breast-pathology-from-image-processing-techniques-to-artificial-intelligence
#15
REVIEW
Stephanie Robertson, Hossein Azizpour, Kevin Smith, Johan Hartman
Breast cancer is the most common malignant disease in women worldwide. In recent decades, earlier diagnosis and better adjuvant therapy have substantially improved patient outcome. Diagnosis by histopathology has proven to be instrumental to guide breast cancer treatment, but new challenges have emerged as our increasing understanding of cancer over the years has revealed its complex nature. As patient demand for personalized breast cancer therapy grows, we face an urgent need for more precise biomarker assessment and more accurate histopathologic breast cancer diagnosis to make better therapy decisions...
November 7, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/29162012/meeting-report-tissue-based-image-analysis
#16
Chandra Saravanan, Vanessa Schumacher, Danielle Brown, Robert Dunstan, Jean-Rene Galarneau, Marielle Odin, Sasmita Mishra
Quantitative image analysis (IA) is a rapidly evolving area of digital pathology. Although not a new concept, the quantification of histological features on photomicrographs used to be cumbersome, resource-intensive, and limited to specialists and specialized laboratories. Recent technological advances like highly efficient automated whole slide digitizer (scanner) systems, innovative IA platforms, and the emergence of pathologist-friendly image annotation and analysis systems mean that quantification of features on histological digital images will become increasingly prominent in pathologists' daily professional lives...
October 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/29155983/meox1-accelerates-myocardial-hypertrophic-decompensation-through-gata4
#17
Dan Lu, Jizheng Wang, Jing Li, Feifei Guan, Xu Zhang, Wei Dong, Ning Liu, Shan Gao, Lianfeng Zhang
Aims: Pathological hypertrophy is the result of gene network regulation, which ultimately leads to adverse cardiac remodelling and heart failure (HF), and is accompanied by the reactivation of a 'foetal gene programme'. The Mesenchyme homeobox 1 (Meox1) gene is one of the foetal programme genes. Meox1 may play a role in embryonic development, but its regulation of pathological hypertrophy is not known. Therefore, this study investigated the effect of Meox1 on pathological hypertrophy, including familial and pressure overload-induced hypertrophy, and its potential mechanism of action...
November 16, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/29154725/non-aneurysmal-subarachnoid-hemorrhage-when-is-a-second-angiography-indicated
#18
Asma Bashir, Ronni Mikkelsen, Leif Sørensen, Niels Sunde
Purpose Repeat imaging in patients with non-aneurysmal subarachnoid hemorrhage (NASAH) remains controversial. We aim to report our experience with NASAH with different hemorrhage patterns, and to investigate the need for further diagnostic workup to determine the underlying cause of hemorrhage. Method We conducted a retrospective analysis of all spontaneous SAH with an initial negative computed tomography (CT) with angiography (CTA) and/or digital subtraction angiography (DSA) from October 2011 through May 2017...
January 1, 2017: Neuroradiology Journal
https://www.readbyqxmd.com/read/29153993/the-high-frequency-of-superior-labrum-biceps-tendon-and-superior-rotator-cuff-pathologies-in-patients-with-subscapularis-tears-a-cohort-study
#19
Muhammet Baybars Ataoglu, Mehmet Cetinkaya, Mustafa Ozer, Tacettin Ayanoglu, Ulunay Kanatli
BACKGROUND: The purpose of this study was to assess the frequency of superior labrum anterior posterior (SLAP) lesions, long head of biceps tendon (LHBT) pathologies, and superior rotator cuff tears accompanying subscapularis tears. We hypothesised that LHBT lesions, superior rotator cuff tears, and especially SLAP lesions were very frequent with subscapularis tears. METHODS: The digital files of patients who underwent shoulder arthroscopy were reviewed retrospectively...
November 16, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/29153365/comparison-of-screening-cedm-and-mri-for-women-at-increased-risk-for-breast-cancer-a-pilot-study
#20
Maxine S Jochelson, Katja Pinker, D David Dershaw, Mary Hughes, Girard F Gibbons, Kareem Rahbar, Mark E Robson, Debra A Mangino, Debra Goldman, Chaya S Moskowitz, Elizabeth A Morris, Janice S Sung
OBJECTIVES: Contrast enhanced digital mammography (CEDM) is a new breast imaging technology increasingly used in the diagnostic setting but its utility in the pure screening setting has not been reported. The goal of this pilot study is to prospectively compare screening CEDM to breast MRI in women with an increased risk for breast cancer. METHODS: In this IRB-approved HIPAA-compliant study, 318 women at increased breast cancer risk were consented (December 2012-May 2015) to undergo CEDM in addition to their scheduled MRI...
December 2017: European Journal of Radiology
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