keyword
MENU ▼
Read by QxMD icon Read
search

measurement

keyword
https://www.readbyqxmd.com/read/28330313/optimization-of-process-parameters-for-enhanced-production-of-jamun-juice-using-pectinase-aspergillus-aculeatus-enzyme-and-its-characterization
#1
Payel Ghosh, Rama Chandra Pradhan, Sabyasachi Mishra
Jamun fruit comprises of seed and thick pulp. The pectin-protein bond of the thick pulp creates difficulty in making juice. Clear Jamun juice is not available in the market, so there is a need for extraction of juice with maximum yield. The goal of this research is to obtain high yield of clarified juice with the help of Pectinase (Aspergillus aculeatus) enzyme. The study was conducted at different enzyme concentration (0.01-0.1%), time duration (40-120 min), and temperature (30-50 °C). Various physical and chemical parameters, such as yield, turbidity, viscosity, clarity, colour, polyphenol, protein, TSS, and total solid, were measured as dependent variables...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330312/isolation-and-screening-of-l-asparaginase-free-of-glutaminase-and-urease-from-fungal-sp
#2
Kruthi Doriya, Devarai Santhosh Kumar
L-Asparaginase is a chemotherapeutic drug used in the treatment of acute lymphoblastic leukaemia (ALL), a malignant disorder in children. L-Asparaginase helps in removing acrylamide found in fried and baked foods that is carcinogenic in nature. L-Asparaginase is present in plants, animals and microbes. Various microorganisms such as bacteria, yeast and fungi are generally used for the production of L-asparaginase as it is difficult to obtain the same from plants and animals. L-Asparaginase from bacteria causes anaphylaxis and other abnormal sensitive reactions due to low specificity to asparagine...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330291/optimization-of-culture-conditions-by-response-surface-methodology-and-unstructured-kinetic-modeling-for-bioactive-metabolite-production-by-nocardiopsis-litoralis-vsm-8
#3
Ushakiranmayi Managamuri, Muvva Vijayalakshmi, Sudhakar Poda, V S Rama Krishna Ganduri, R Satish Babu
Response surface methodology-based central composite design on five variables incubation time, pH, temperature, sucrose concentration, and soya peptone concentration was employed for optimization of the production of bioactive compounds by Nocardiopsis litoralis strain VSM 8. The main quadratic effects and interactions of the five variables on the production of bioactive metabolites were investigated. A second-order polynomial model produced a satisfied fit for experimental data with regard to the production of the bioactive metabolites...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330286/effect-of-highly-branched-hyphal-morphology-on-the-enhanced-production-of-cellulase-in-trichoderma-reesei-des-15
#4
Ronglin He, Chen Li, Lijuan Ma, Dongyuan Zhang, Shulin Chen
The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330271/assessment-of-protein-silver-nanoparticles-toxicity-against-pathogenic-alternaria-solani
#5
Sobhy I I Abdel-Hafez, Nivien A Nafady, Ismail R Abdel-Rahim, Abeer M Shaltout, José-Antonio Daròs, Mohamed A Mohamed
Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract of endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were characterized by means of spectroscopic and microscopic techniques. The surface plasmon resonance found at 430 nm confirmed the formation of stable AgNPs for several weeks at room temperature. Also, the results revealed the formation of spherical and monodispersed AgNPs with an average size of 14...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330248/photo-inactivation-of-escherichia-coli-and-enterococcus-hirae-using-methylene-blue-and-sodium-anthraquinone-2-sulphonate-effect-of-process-parameters
#6
Madhavi Singh, Kannan Pakshirajan, Vishal Trivedi
In this study, effect of different parameters, viz. concentration of photosensitizer (PS), pH of the bacterial cell suspension and initial cell count, on photo-inactivation of Escherichia coli and Enterococcus hirae bacteria using methylene blue (MB) and sodium anthraquinone-2-sulphonate (SAQS) was investigated employing the statistically valid full factorial design of experiments. The inactivation efficiency of E. hirae using MB ranges between 10.81 and 48.55 %, whereas in the case of E. coli it ranges between 10...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330247/production-of-glucosinolates-phenolic-compounds-and-associated-gene-expression-profiles-of-hairy-root-cultures-in-turnip-brassica-rapa-ssp-rapa
#7
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR. GSLs levels (glucoallysin, glucobrassicanapin, gluconasturtiin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and 4-hydroxyglucobrassicin) and their gene expression levels (BrMYB28, BrMYB29, BrMYB34, BrMYB51, BrMYB122, CYP79 and CYP83) significantly increased in hairy roots compared with that in non-transformed roots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330234/optimization-of-cellulase-production-by-penicillium-sp
#8
H N Prasanna, G Ramanjaneyulu, B Rajasekhar Reddy
The production of cellulolytic enzymes (β-exoglucanase, β-endoglucanase and β-glucosidase) by Penicillium sp. on three different media in liquid shake culture conditions was compared. The organism exhibited relatively highest activity of endoglucanase among three enzymes measured at 7-day interval during the course of its growth on Czapek-Dox medium supplemented with 0.5 % (w/v) cellulose. Cellulose at 0.5 %, lactose at 0.5 %, sawdust at 0.5 %, yeast extract at 0.2 % as a nitrogen source, pH 5.0 and 30 °C temperature were found to be optimal for growth and cellulase production by Penicillium sp...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330209/biodegradation-of-real-petroleum-wastewater-by-immobilized-hyper-phenol-tolerant-strains-of-bacillus-cereus-in-a-fluidized-bed-bioreactor
#9
Aditi Banerjee, Aloke K Ghoshal
Microbial bioremediation of petroleum wastewater by phenol-degrading-bacteria holds promise in circumventing the issue of petroleum-spill related pollution. Herein, biodegradation of petroleum wastewater samples collected from oil refinery site was carried out in a fluidized bed bioreactor by Ca-alginate immobilized biomass of phenol-degrading strains of Bacillus cereus (AKG1 MTCC9817 and AKG2 MTCC9818). Degradation performance of the system was evaluated by measuring the changes in chemical oxygen demand (COD) and level of phenolic compounds in the wastewater samples during the microbial treatment...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330204/endophytic-fungal-isolate-mediated-biosynthesis-of-silver-nanoparticles-and-their-free-radical-scavenging-activity-and-anti-microbial-studies
#10
Vasudeva Reddy Netala, Venkata Subbaiah Kotakadi, Pushpalatha Bobbu, Susmila Aparna Gaddam, Vijaya Tartte
The present study reports that the biosynthesis of AgNPs using an endophytic fungus isolated from the ethnomedicinal plant Centella asiatica. The endophytic fungus was identified as Aspergillus versicolor ENT7 based on 18S rRNA gene sequencing (NCBI Accession number KF493864). The AgNPs synthesized were characterized by UV-visible spectroscopy, Fourier transform infra-red spectroscopy (FTIR), transmission electron microscopy (TEM), X-ray diffraction (XRD), particle size analyzer, and zeta potential measurements...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330162/evaluation-of-silver-nanoparticles-synthetic-potential-of-couroupita-guianensis-aubl-flower-buds-extract-and-their-synergistic-antibacterial-activity
#11
T Venkata Rajesh Kumar, J S R Murthy, Madamsetti Narayana Rao, Y Bhargava
The present investigation demonstrates Couroupita guianensis flower buds extract mediated synthesis of stable silver nanoparticles (AgNPs). Instant formation of AgNPs was primarily confirmed by the appearance of yellowish brown colour and characteristic silver SPR band in the UV-visible spectrum. Elemental and crystalline natures of the AgNPs were identified from EDX and XRD pattern, respectively. Spherical morphology and the mono-disparity were revealed from TEM and AFM images. The particle size ranged from 5 to 30 nm and average size of 17 nm was consistent in XRD, TEM and AFM measurements...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330155/purification-and-characterization-of-rga2-a-rho2-gtpase-activating-protein-from-tinospora-cordifolia
#12
Mohd Amir, Mohammad Aasif Dar, Wahiduzzaman, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Rho GTPases activating protein 2 (RGA2) is primarily involved in the modulation of numerous morphological events in eukaryotes. It protects plants by triggering the defense system which restricts the pathogen growth. This is the first report on the isolation, purification and characterization of RGA2 from the stems of Tinospora cordifolia, a medicinal plant. The RGA2 was purified using simple two-step process using DEAE-Hi-Trap FF and Superdex 200 chromatography columns, with a high yield. The purity of RGA2 was confirmed by SDS-PAGE and identified by MALDI-TOF/MS...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#13
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329935/-a-cross-sectional-survey-of-receiving-no-methadone-maintenance-treatment-in-hiv-infected-injecting-drug-users-in-dehong-dai-and-jingpo-autonomous-prefecture-yunnan-province
#14
R H Tang, R H Ye, Y C Yang, S T Yao, J B Wang, R M Zhang, L F Luo, Z L Wu, Y C Long, M S Yin, N He, S Duan
Objective: To understand the current status of receiving no methadone maintenance treatment (MMT) and influencing factors in HIV infected injecting drug users (IDUs) in Dehong Dai and Jingpo autonomous prefectures, Yunnan province. Methods: Data of survival of IDUs with AIDS in Dehong were collected from " Chinese National Comprehensive HIV/AIDS and Care Information System" in December, 2014. Results: There were 987 IDUs who should receive MMT, the majority of them were males (94.6%, 934/987), aged 35-44 years (53...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329930/-commuting-mode-specific-exposure-to-pm-2-5-in-urban-area-of-guangzhou
#15
X J Lyu, Z H Li, X Li, W L Zeng, P Yang, Q X Lin, J Y Zheng, X L Du, Y Z Gu, Y Q Zhao, R S Xie, T Liu, H L Lin, W J Ma
Objective: To find the differences in PM(2.5) exposure level in the context of four commuting modes (by walk, bicycle, bus and subway) in Guangzhou. Methods: The PM(2.5) exposure assessment was carried out from January to December 2015 in Guangzhou. PM(2.5) was measured by using SidePak individual dust meter (AM510, TSI Inc. USA) with time interval of 1 minute. Our measurement was taken on Monday, Wednesday, Friday and Sunday in the second week of each month and the samples were collected in the morning (07:00-09:00), afternoon (11:00-13:00) and evening (17:00-19:00)...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329927/-lagged-effects-of-diurnal-temperature-range-on-mortality-in-66-cities-in-china-a-time-series-study
#16
Y Q Zhao, L J Wang, Y Luo, P Yin, Z J Huang, T Liu, H L Lin, J P Xiao, X Li, W L Zeng, W J Ma, M G Zhou
Objective: To estimate the effect of daily diurnal temperature range (DTR) on mortality in different areas in China. Methods: A time series study using the data collected from 66 areas in China was conducted, and Meta-analysis was used to analyze the estimates of associations between DTR and daily mortality. Modifying effects of extremely low and high DTR-mortality relationship by season and socioeconomic status (SES) were also evaluated respectively. Cumulative excess risk (CER) was used as an index to evaluate the effects...
March 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28329909/testing-the-boundaries-of-boundary-extension-anticipatory-scene-representation-across-development-and-disorder
#17
G Spanò, H Intraub, J O Edgin
Recent studies have suggested that Boundary Extension (BE), a scene construction error, may be linked to the function of the hippocampus. In this study, we tested BE in two groups with variations in hippocampal development and disorder: a typically developing sample ranging from preschool to adolescence and individuals with Down syndrome. We assessed BE across three different test modalities: drawing, visual recognition, and a 3D scene boundary reconstruction task. Despite confirmed fluctuations in memory function measured through a neuropsychological assessment, the results showed consistent BE in all groups across test modalities, confirming the near universal nature of BE...
March 22, 2017: Hippocampus
https://www.readbyqxmd.com/read/28329874/long-term-health-related-quality-of-life-outcomes-in-digital-replantation-versus-revision-amputation
#18
Oren Tessler, Matthew J Bartow, Marie P Tremblay-Champagne, Alex M Lin, Geneviève Landes, Sarah Sebbag, Andreas Nikolis
Background Earlier, digit viability judged the success of digital replantation. Now, utility health-related quality of life (HRQOL) measures can better assess the impact of digital replantation. Methods Overall, 264 digital injury patients were sent a regimen of utility measures: Disabilities of the Arm, Shoulder and Hand (DASH) score, European Quality of Life 5 Dimensions, visual analog scale (VAS), time trade-off (TTO), and standard gamble (SG). Overall, 51 patients responded completely to all of these-36 replantation patients and 15 revision amputation patients...
March 22, 2017: Journal of Reconstructive Microsurgery
https://www.readbyqxmd.com/read/28329867/effectiveness-of-a-virtual-reality-forest-on-people-with-dementia-a-mixed-methods-pilot-study
#19
Wendy Moyle, Cindy Jones, Toni Dwan, Tanya Petrovich
Purpose of the Study: To measure and describe the effectiveness of a Virtual Reality Forest (VRF) on engagement, apathy, and mood states of people with dementia, and explore the experiences of staff, people with dementia and their families. Design and Methods: A mixed-methods study conducted between February and May 2016. Ten residents with dementia, 10 family members, and 9 care staff were recruited from 2 residential aged care facilities, operated by one care provider, located in Victoria, Australia...
March 15, 2017: Gerontologist
https://www.readbyqxmd.com/read/28329864/age-differences-in-cognitive-and-affective-theory-of-mind-concurrent-contributions-of-neurocognitive-performance-sex-and-pulse-pressure
#20
Ashley L Fischer, Norm O'Rourke, Wendy Loken Thornton
Objectives: Theory of mind (ToM) allows us to detect and make inferences about cognitive and affective mental states. Mixed findings exist regarding (a) age differences in cognitive and affective ToM and (b) what mechanisms may underlie changes in the two components. We addressed these questions by examining the unique and joint contributions of neurocognitive performance, pulse pressure (PP), and biological sex to age differences in cognitive and affective ToM. Method: We tested 86 young and 85 older adults on standardized measures of neurocognitive performance and ToM...
August 7, 2016: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
keyword
keyword
43340
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"