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https://www.readbyqxmd.com/read/28757159/effectivity-of-oral-recombinant-dna-vaccine-against-streptococcus-agalactiae-in-nile-tilapia
#1
Ling Zhu, Qian Yang, Lingyuan Huang, Kaiyu Wang, Xingli Wang, Defang Chen, Yi Geng, Xiaoli Huang, Ping Ouyang, Weimin Lai
The effects of oral vaccination by two immune routes (gavage and mixed fodder administration) using a recombinant DNA vaccine SL7207-pVAX1-sip against Streptococcus agalactiae were assessed. SL7207-pVAX1-sip significantly enhanced various innate immune responses of tilapia, such as total serum protein, superoxide dismutase activity, lysozyme activity, complement C3 concentration in serum, serum antibacterial activity, and interleukin 1β and tumor necrosis factor-α. Vaccinated fish had higher relative percent survival values (57% and 63% for gavage group and mix fodder administration, respectively) at 10 weeks after S...
July 28, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28724462/longitudinal-analyses-of-correlated-response-efficiencies-of-fillet-traits-in-nile-tilapia
#2
E M Turra, A F A Fernandes, E R de Alvarenga, E A Teixeira, G F O Alves, L G Manduca, T W Murphy, M A Silva
Recent studies with Nile tilapia have shown divergent results regarding the possibility of selecting on morphometric measurements to promote indirect genetic gains in fillet yield (FY). The use of indirect selection for fillet traits is important as these traits are only measurable after harvesting. Random regression models are a powerful tool in association studies to identify the best time point to measure and select animals. Random regression models can also be applied in a multiple trait approach to analyze indirect response to selection, which would avoid the need to sacrifice candidate fish...
July 20, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28716034/growth-arrest-specific-gene-2-in-tilapia-oreochromis-niloticus-molecular-characterization-and-functional-analysis-under-low-temperature-stress
#3
ChangGeng Yang, Fan Wu, Xing Lu, Ming Jiang, Wei Liu, Lijuan Yu, Juan Tian, Hua Wen
BACKGROUND: Growth arrest specific 2 (gas2) gene is a component of the microfilament system that plays a major role in the cell cycle, regulation of microfilaments, and cell morphology during apoptotic processes. However, little information is available on fish gas2. In this study, the tilapia (Oreochromis niloticus) gas2 gene was cloned and characterized for the first time. RESULTS: The open reading frame was 1020 bp, encoding 340 amino acids; the 5'-untranslated region (UTR) was 140 bp and the 3'-UTR was 70 bp, with a poly (A) tail...
July 17, 2017: BMC Molecular Biology
https://www.readbyqxmd.com/read/28666865/different-%C3%AE-glucans-improve-the-growth-performance-and-bacterial-resistance-in-nile-tilapia
#4
Fabiana Pilarski, Carlos Alberto Ferreira de Oliveira, Fernanda Patrícia Brito Darpossolo de Souza, Fábio Sabbadin Zanuzzo
The role of glucan as a biologically active immunomodulator has been well documented for more than 40 years. However, the wide diversity of β-glucan forms and the extraction process has implications for the benefits of these compounds. Biorigin developed two samples of β-glucans using different biotechnological processes. Thus, in the present study, we fed Nile tilapia (Oreochromis niloticus) diets containing these two β-glucan molecules (BG01 and BG02) for 30 days prior to bacterial infection with Streptococcus agalactiae...
June 27, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28652133/acute-salinity-tolerance-and-the-control-of-two-prolactins-and-their-receptors-in-the-nile-tilapia-oreochromis-niloticus-and-mozambique-tilapia-o-mossambicus-a-comparative-study
#5
Yoko Yamaguchi, Jason P Breves, Maria C Haws, Darren T Lerner, E Gordon Grau, Andre P Seale
Osmoregulation in vertebrates is largely controlled by the neuroendocrine system. Prolactin (PRL) is critical for the survival of euryhaline teleosts in fresh water by promoting ion retention. In the euryhaline Mozambique tilapia (Oreochromis mossambicus), pituitary PRL cells release two PRL isoforms, PRL188 and PRL177, in response to a fall in extracellular osmolality. Both PRLs function via two PRL receptors (PRLRs) denoted PRLR1 and PRLR2. We conducted a comparative study using the Nile tilapia (O. niloticus), a close relative of Mozambique tilapia that is less tolerant to increases in environmental salinity, to investigate the regulation of PRLs and PRLRs upon acute hyperosmotic challenges in vivo and in vitro...
June 23, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28390071/preparation-and-bioavailability-of-calcium-chelating-peptide-complex-from-tilapia-skin-hydrolysates
#6
Jun Chen, Xujian Qiu, Gengxin Hao, Meng Zhang, Wuyin Weng
BACKGROUND: With the continuous improvement in material life, the generation of fish by-products and the market demand for calcium supplements have been increasing in China. Therefore a calcium-chelating peptide complex (CPC) from tilapia skins was prepared and its effect on calcium (Ca)-deficient mice was investigated. RESULTS: The molecular weight distribution of CPC mainly ranged from 2000 to 180 Da, and its contents of complete amino acids and free amino acids were 85...
April 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28266220/metabolic-mechanism-for-l-leucine-induced-metabolome-to-eliminate-streptococcus-iniae
#7
Chao-Chao Du, Man-Jun Yang, Min-Yi Li, Jun Yang, Bo Peng, Hui Li, Xuan-Xian Peng
Crucial metabolites that modulate hosts' metabolome to eliminate bacterial pathogens have been documented, but the metabolic mechanisms are largely unknown. The present study explores the metabolic mechanism for l-leucine-induced metabolome to eliminate Streptococcus iniae in tilapia. GC-MS-based metabolomics was used to investigate the tilapia liver metabolic profile in the presence of exogenous l-leucine. Thirty-seven metabolites of differential abundance were determined, and 11 metabolic pathways were enriched...
May 5, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28230233/establishment-and-growth-responses-of-nile-tilapia-embryonic-stem-like-cell-lines-under-feeder-free-condition
#8
Zhenhua Fan, Linyan Liu, Xiaohuan Huang, Yang Zhao, Linyan Zhou, Deshou Wang, Jing Wei
Embryonic stem (ES) cells provide an invaluable tool for molecular analysis of vertebrate development and a bridge linking genomic manipulations in vitro and functional analysis of target genes in vivo. Work towards fish ES cells so far has focused on zebrafish (Danio renio) and medaka (Oryzias latipes). Here we describe the derivation, pluripotency, differentiation and growth responses of ES cell lines from Nile tilapia (Oreochromis niloticus), a world-wide commercial farmed fish. These cell lines, designated as TES1-3, were initiated from blastomeres of Nile tilapia middle blastula embryos (MBE)...
February 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28062189/evaluation-of-marine-psychrophile-psychrobacter-namhaensis-so89-as-a-probiotic-in-nile-tilapia-oreochromis-niloticus-diets
#9
Sarah O Makled, Amira M Hamdan, Abdel-Fattah M El-Sayed, Elsayed E Hafez
Marine environment represents a promising source of new, unconventional bioactive compounds with health-promoting abilities, which can be used as food supplements. The present study was carried out to evaluate the efficacy of marine Psychrobacter namhaensis SO89 on growth performance and immune response of Nile tilapia (Oreochromis niloticus). P. namhaensis were isolated from marine environments and phylogenetically identified by 16S rRNA gene sequences. The bacterial isolate was incorporated in Nile tilapia diets (30% crude protein) at three concentrations (0...
February 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28028640/effect-of-dietary-probiotic-supplementation-on-intestinal-microbiota-and-physiological-conditions-of-nile-tilapia-oreochromis-niloticus-under-waterborne-cadmium-exposure
#10
Qixiao Zhai, Leilei Yu, Tianqi Li, Jiamin Zhu, Chengcheng Zhang, Jianxin Zhao, Hao Zhang, Wei Chen
The heavy metal cadmium (Cd) is a hazardous pollutant that exerts various toxic effects on aquatic animals. The biomagnifying effects of this non-essential element in the food chain also pose threats to human health. In this study, the protective effect of a dietary probiotic supplementation, Lactobacillus plantarum CCFM8610, on the intestinal microbiota and physiological conditions of Nile tilapia (Oreochromis niloticus) exposed to waterborne Cd was evaluated. Two hundred fish were divided into four groups, i...
April 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27988351/duplication-of-neuropeptide-y-and-peptide-yy-in-nile-tilapia-oreochromis-niloticus-and-their-roles-in-food-intake-regulation
#11
Peipei Yan, Jirong Jia, Guokun Yang, Dongfang Wang, Caiyun Sun, Wensheng Li
In vertebrates, the neuropeptide Y (NPY) family peptides have been recognized as key players in food intake regulation. NPY centrally promotes feeding, while peptide YY (PYY) and pancreatic polypeptide (PP) mediate satiety. The teleost tetraploidization is well-known to generate duplicates of both NPY and PYY; however, the functional diversification between the duplicate genes, especially in the regulation of food intake, remains unknown. In this study, we identified the two duplicates of NPY and PYY in Nile tilapia (Oreochromis niloticus)...
February 2017: Peptides
https://www.readbyqxmd.com/read/27973565/nutritional-profile-and-chemical-stability-of-pasta-fortified-with-tilapia-oreochromis-niloticus-flour
#12
Maria Lúcia G Monteiro, Eliane T Mársico, Manoel S Soares, Amanda O Magalhães, Anna Carolina V C S Canto, Bruno R C Costa-Lima, Thiago S Alvares, Carlos A Conte
Physicochemical parameters of pasta enriched with tilapia (Oreochromis niloticus) flour were investigated. Five formulations were prepared with different concentrations of tilapia flour as partial substitute of wheat flour: pasta without tilapia flour (PTF0%), pasta with 6% (PTF6%), 12% (PTF12%), 17% (PTF17%), and 23% (PTF23%) of tilapia flour. The formulations were assessed for proximate composition, fatty acid and amino acid profile on day 1 whereas, instrumental color parameters (L*, a* and b* values), pH, water activity (aw), and lipid and protein oxidation were evaluated on days 1, 7, 14, and 21 of storage at 25°C...
2016: PloS One
https://www.readbyqxmd.com/read/27965164/glucose-enhances-tilapia-against-edwardsiella-tarda-infection-through-metabolome-reprogramming
#13
Zhao-Hai Zeng, Chao-Chao Du, Shi-Rao Liu, Hui Li, Xuan-Xian Peng, Bo Peng
We have recently reported that the survival of tilapia, Oreochromis niloticus, during Edwardsiella tarda infection is tightly associated with their metabolome, where the survived O. niloticus has distinct metabolomic profile to dying O. niloticus. Glucose is the key metabolite to distinguish the survival- and dying-metabolome. More importantly, exogenous administration of glucose to the fish greatly enhances their survival for the infection, indicating the functional roles of glucose in metabolome repurposing, known as reprogramming metabolomics...
February 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27855320/mir-122-promotes-hepatic-antioxidant-defense-of-genetically-improved-farmed-tilapia-gift-oreochromis-niloticus-exposed-to-cadmium-by-directly-targeting-a-metallothionein-gene
#14
Jun Qiang, Yi-Fan Tao, Jie He, Pao Xu, Jin-Wen Bao, Yi-Lan Sun
MicroRNAs (miRNAs) are small, non-coding RNAs that regulate target gene expression by binding to the 3'untranslated region (3'UTR) of the target mRNA. MiRNAs regulate a large variety of genes, including those involved in liver homeostasis and energy metabolism. Down-regulated levels of hepatic miR-122 were found in genetically improved farmed tilapia (GIFT, Oreochromis niloticus) exposed to cadmium (Cd) stress. Here, we report for the first time that reduction of miR-122 post-transcriptionally increased metallothionein (MT) mRNA levels by binding to its 3'UTR, as shown by a 3' UTR luciferase reporter assay...
January 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/27800707/characterization-of-tilapia-oreochromis-niloticus-aldehyde-reductase-akr1a1-gene-promoter-and-expression-pattern-in-benzo-a-pyrene-exposed-fish
#15
Abeer Hassanin, Yoshio Kaminishi, Takao Itakura
This study planned to isolation and characterization of AKR1A1 cDNA from Bap injected nile tilapia (Oreochromis niloticus), comparison of its characteristic structures with those of other species, characterization of AKR1A1 gene and promoter, and investigation of AKR1A1 mRNA expression in various organs of Bap injected tilapia. The cDNA was 1172 bp long which includes an open reading frame of 975 bp encoding a 324 amino acids protein and a stop codon. The sequence showed 3' and 5' non-coding regions of 179 and 18 bp...
January 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/27782217/genome-wide-methylation-analysis-identified-sexually-dimorphic-methylated-regions-in-hybrid-tilapia
#16
Zi Yi Wan, Jun Hong Xia, Grace Lin, Le Wang, Valerie C L Lin, Gen Hua Yue
Sexual dimorphism is an interesting biological phenomenon. Previous studies showed that DNA methylation might play a role in sexual dimorphism. However, the overall picture of the genome-wide methylation landscape in sexually dimorphic species remains unclear. We analyzed the DNA methylation landscape and transcriptome in hybrid tilapia (Oreochromis spp.) using whole genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq). We found 4,757 sexually dimorphic differentially methylated regions (DMRs), with significant clusters of DMRs located on chromosomal regions associated with sex determination...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27717900/identification-of-a-c-type-lectin-from-tilapia-oreochromis-niloticus-and-its-functional-characterization-under-low-temperature-stress
#17
ChangGeng Yang, Ming Jiang, Fan Wu, Lijuan Yu, Juan Tian, Wei Liu, Xing Lu, Hua Wen
C-type lectin, which plays an important role in fish innate immunity, was cloned from tilapia and its functional characterization under low-temperature stress is reported. Its ORF is 453 bp, encoding 150 amino acids, and has a 5'UTR of 83 bp, a 3'UTR of 559 bp, and a poly (A) tail. The tilapia C-type lectin genomic DNA was acquired with a length of 5714 bp, containing six exons and five introns. Its promoter sequence was cloned and has a length of 2251 bp. The highest promoter activity occurs in the regulatory region (-900 bp to -450 bp)...
November 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27664336/uplc-ms-ms-determination-of-florfenicol-and-florfenicol-amine-antimicrobial-residues-in-tilapia-muscle
#18
Eduardo Adilson Orlando, Aline Gabriela Costa Roque, Marcos Eliseu Losekann, Ana Valéria Colnaghi Simionato
Despite the benefits to fish farmers, the use of antimicrobials in aquaculture has concerned consumers and competent authorities. The indiscriminate use of such substances promotes the emergence of resistant microorganisms, decreases the effectiveness of treatments, and causes possible toxic effects in humans. In Brazil, florfenicol is the only antimicrobial registered for use in aquaculture and is often used in tilapia in cage creation. Thus, this study aimed to develop a method for determination of florfenicol residues and its metabolite florfenicol amine in tilapia fillet by UPLC-MS/MS...
November 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27565293/microbial-chitosan-as-a-biopreservative-for-fish-sausages
#19
Ahmed A Tayel
Processed fish products are worthy sources to supply man with his main nutritional needs, but they are extremely susceptible to quality loss during storage. Microbial (fungal) chitosan is a bioactive polymer that has numerous applications in health promoting fields. Fungal chitosan was extracted from the grown mycelia of Aspergillus brasiliensis (niger) to investigate its potential role as antimicrobial, preservative and quality improvement agent, in processed fish sausages from Nile tilapia (Oreochromis niloticus)...
December 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27486872/global-dna-methylation-changes-in-nile-tilapia-gonads-during-high-temperature-induced-masculinization
#20
Li-Xue Sun, Yi-Ya Wang, Yan Zhao, Hui Wang, Ning Li, Xiang Shan Ji
In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing...
2016: PloS One
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