Read by QxMD icon Read

Tilapia promoter

ChangGeng Yang, Ming Jiang, Fan Wu, Lijuan Yu, Juan Tian, Wei Liu, Xing Lu, Hua Wen
C-type lectin, which plays an important role in fish innate immunity, was cloned from tilapia and its functional characterization under low-temperature stress is reported. Its ORF is 453 bp, encoding 150 amino acids, and has a 5'UTR of 83 bp, a 3'UTR of 559 bp, and a poly (A) tail. The tilapia C-type lectin genomic DNA was acquired with a length of 5714 bp, containing six exons and five introns. Its promoter sequence was cloned and has a length of 2251 bp. The highest promoter activity occurs in the regulatory region (-900 bp to -450 bp)...
October 4, 2016: Fish & Shellfish Immunology
Eduardo Adilson Orlando, Aline Gabriela Costa Roque, Marcos Eliseu Losekann, Ana Valéria Colnaghi Simionato
Despite the benefits to fish farmers, the use of antimicrobials in aquaculture has concerned consumers and competent authorities. The indiscriminate use of such substances promotes the emergence of resistant microorganisms, decreases the effectiveness of treatments, and causes possible toxic effects in humans. In Brazil, florfenicol is the only antimicrobial registered for use in aquaculture and is often used in tilapia in cage creation. Thus, this study aimed to develop a method for determination of florfenicol residues and its metabolite florfenicol amine in tilapia fillet by UPLC-MS/MS...
November 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ahmed A Tayel
Processed fish products are worthy sources to supply man with his main nutritional needs, but they are extremely susceptible to quality loss during storage. Microbial (fungal) chitosan is a bioactive polymer that has numerous applications in health promoting fields. Fungal chitosan was extracted from the grown mycelia of Aspergillus brasiliensis (niger) to investigate its potential role as antimicrobial, preservative and quality improvement agent, in processed fish sausages from Nile tilapia (Oreochromis niloticus)...
August 24, 2016: International Journal of Biological Macromolecules
Li-Xue Sun, Yi-Ya Wang, Yan Zhao, Hui Wang, Ning Li, Xiang Shan Ji
In some fish species, high or low temperature can switch the sex determination mechanisms and induce fish sex reversal when the gonads are undifferentiated. During this high or low temperature-induced sex reversal, the expressions of many genes are altered. However, genome-wide DNA methylation changes in fish gonads after high or low temperature treatment are unclear. Herein, we compared the global DNA methylation changes in the gonads from control females (CF), control males (CM), high temperature-treated females (TF), and high temperature-induced males (IM) from the F8 family of Nile tilapia (Oreochromis niloticus) using methylated DNA immunoprecipitation sequencing...
2016: PloS One
Jonathan D Douros, David A Baltzegar, Jamie Mankiewicz, Jordan Taylor, Yoko Yamaguchi, Darren T Lerner, Andre P Seale, E Gordon Grau, Jason P Breves, Russell J Borski
Leptin is an important cytokine for regulating energy homeostasis, however, relatively little is known about its function and control in teleost fishes or other ectotherms, particularly with regard to interactions with the growth hormone (GH)/insulin-like growth factors (IGFs) growth regulatory axis. Here we assessed the regulation of LepA, the dominant paralog in tilapia (Oreochromis mossambicus) and other teleosts under altered nutritional state, and evaluated how LepA might alter pituitary growth hormone (GH) and hepatic insulin-like growth factors (IGFs) that are known to be disparately regulated by metabolic state...
July 19, 2016: General and Comparative Endocrinology
Samah R Khalil, Yasser Abd Elhakim, Abd Elhakeem El-Murr
Increase in consumption of consumer items such as plasticizers have resulted in a sharp rise in the presence of xenobiotics like phthalic acid esters (PEs) in freshwater and marine environments due to contaminated runoff and improper release of effluents. The sublethal toxicity of Di-n-butyl phthalate (DBP) was investigated in juvenile Nile tilapia, Oreochromis niloticus, in an attempt to determine the biological effect of exposure to 1/2 and 1/3 median lethal concentration (96-h LC50) which, in our study was experimentally determined to be 11...
February 2016: Japanese Journal of Veterinary Research
Yingli Shi, Jianhai Xiang, Guangzhou Zhou, Tetsuzan Benny Ron, Hsin-I Tong, Wen Kang, Si Sun, Yuanan Lu
A newly isolated Pacific white shrimp (Litopenaeus vannamei) beta-actin promoter SbaP and its derivative compact construct SbaP (ENX) have recently been demonstrated to promote ectopic gene expression in vitro and in vivo. To further explore the potential transduction application, this newly isolated shrimp promoter SbaP was comparatively tested with cytomegalovirus (CMV), simian virus 40 (SV40), polyhedrin (Polh), and white spot syndrome virus immediate early gene 1 (WSSV ie1) four constitutive promoters and a beta-actin promoter (TbaP) from tilapia fish to characterize its promoting function in eight different cell lines...
June 2016: Marine Biotechnology
Jun Hu, Chao Ran, Suxu He, Yanan Cao, Bin Yao, Yuantu Ye, Xuezhen Zhang, Zhigang Zhou
The present study evaluated the effects of dietary microbial phytase on the growth and gut health of hybrid tilapia (Oreochromis niloticus ♀×Oreochromis aureus ♂), focusing on the effect on intestinal histology, adhesive microbiota and expression of immune-related cytokine genes. Tilapia were fed either control diet or diet supplemented with microbial phytase (1000 U/kg). Each diet was randomly assigned to four groups of fish reared in cages (3×3×2 m). After 12 weeks of feeding, weight gain and feed conversion ratio of tilapia were not significantly improved by dietary microbial phytase supplementation...
June 2016: British Journal of Nutrition
Tian Zhou, Nanping Wang, Yang Xue, Tingting Ding, Xin Liu, Xiumei Mo, Jiao Sun
The development of biomaterials with the ability to induce skin wound healing is a great challenge in biomedicine. In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides. It did not change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4(+)/CD8(+) lymphocytes, and the level of IgG or IgM in Sprague-Dawley rats. The tensile strength and contact angle of collagen nanofibers were 6...
July 1, 2016: Colloids and Surfaces. B, Biointerfaces
Dong-Neng Jiang, Hui-Hui Yang, Ming-Hui Li, Hong-Juan Shi, Xian-Bo Zhang, De-Shou Wang
Gonadal soma-derived factor (gsdf) is critical for testicular differentiation in teleosts, yet detailed analysis of Gsdf on testicular differentiation is lacking. In the present study, we knocked out tilapia gsdf using CRISPR/Cas9. F0 gsdf-deficient XY fish with high mutation rate (≥58%) developed as intersex, with ovotestes 90 days after hatching (dah), and become completely sex-reversed with ovaries at 180 and 240 dah. Those individuals with a low mutation rate (<58%) and XY gsdf(+/-) fish developed as males with normal testes...
June 2016: Molecular Reproduction and Development
M A D Dias, R T F de Freitas, S E Arranz, G V Villanova, A W S Hilsdorf
Different strains of Nile tilapia can be found worldwide. To successfully use them in breeding programs, they must be genetically characterized. In this study, four strains of Nile tilapia - UFLA, GIFT, Chitralada and Red-Stirling - were genetically characterized using 10 noncoding microsatellite loci and two microsatellites located in the promoter and first intron of the growth hormone gene (GH). The two microsatellites in the GH gene were identified at positions -693 to -679 in the promoter [motif (ATTCT)8 ] and in intron 1 at positions +140 to +168 [motif (CTGT)7 ]...
June 2016: Animal Genetics
Hongzhen Liu, Jiaxin Zhang, Jianfeng Li, Jinyun Song, Shuangquan Zhang
B cell-activating factor (BAFF)is a member of the tumor necrosis factor (TNF) family and plays roles in B cell survival and maturation. In this study, the full-length cDNA of Nile tilapia (Oreochromis niloticus) BAFF (tBAFF) was amplified from the spleen by reverse transcription PCR (RT-PCR). The open reading frame of this cDNA encodes a protein of 261 amino acids containing a predicted transmembrane domain and a furin protease cleavage site, similar to mammalian, avian, and reptile BAFF. Real-time quantitative PCR (qPCR) analysis revealed that tBAFF is present in various tissues and is predominantly expressed in the spleen...
April 2016: Fish & Shellfish Immunology
Gabriela Hernández-Puga, Pamela Navarrete-Ramírez, Arturo Mendoza, Aurora Olvera, Patricia Villalobos, Aurea Orozco
T3 and cortisol activate or repress gene expression in virtually every vertebrate cell mainly by interacting with their nuclear hormone receptors. In contrast to the mechanisms for hormone gene activation, the mechanisms involved in gene repression remain elusive. In teleosts, the thyroid hormone receptor beta gene or thrb produces two isoforms of TRβ1 that differ by nine amino acids in the ligand-binding domain of the long-TRβ1, whereas the short-TRβ1 lacks the insert. Previous reports have shown that the genomic effects exerted by 3,5-T2, a product of T3 outer-ring deiodination, are mediated by the long-TRβ1...
April 15, 2016: Molecular and Cellular Endocrinology
Magdalena Lenny Situmorang, Peter De Schryver, Kristof Dierckens, Peter Bossier
The growth promoting effect of the bacterial storage compound poly-β-hydroxybutyrate (PHB) has been studied for young fish of high trophic level (European sea bass) and intermediate trophic level (Siberian sturgeon). Here, the effect of PHB on growth, digestive enzyme activities, body composition and diseases resistance of juvenile Nile tilapia (Oreochromis niloticus) of low trophic level was investigated. Although dietary PHB supplementation (5, 25 and 50 g PHB kg(-1) formulated semi-purified diet) during 28 days resulted in a trend of increased weight gain, there was no significant difference in the mean final body weight (258-284 mg) when compared to the fish from the control group (on average 218 mg)...
2016: Veterinary Microbiology
Chao Liu, Jiao Sun
Alveolar bone regeneration has aroused worldwide attention and plays an important role in oral clinics. In recent years, the application of biomaterials to induce osteogenic differentiation of periodontal ligament cells has become the hot topic in the field of alveolar bone regeneration. At present, most existing biomaterials lack osteoinductivity, while extrinsic inducers carry the risk of unwanted side effects. The objective of this work was to study the in vitro functionality of a newly developed hydrolyzed tilapia fish collagen (HFC) for periodontal tissue regeneration...
December 2015: Biomedical Materials
Zana H Kareem, Yasser M Abdelhadi, Annie Christianus, Murni Karim, Nicholas Romano
A 90-day feeding trial was conducted on the growth performance, feeding efficacy, body indices, various hematological and plasma biochemical parameters, and histopathological examination of the gonads from male and female Nile tilapia fingerlings when fed different crude plant extracts from Cinnamomum camphora, Euphorbia hirta, Azadirachta indica, or Carica papaya at 2 g kg(-1) compared to a control diet. This was followed by a 14-day challenge to Streptococcus agalactiae. All treatments were triplicated, and each treatment consisted of 30 fish...
April 2016: Fish Physiology and Biochemistry
Minghui Li, Yunlv Sun, Jiue Zhao, Hongjuan Shi, Sheng Zeng, Kai Ye, Dongneng Jiang, Linyan Zhou, Lina Sun, Wenjing Tao, Yoshitaka Nagahama, Thomas D Kocher, Deshou Wang
Variation in the TGF-β signaling pathway is emerging as an important mechanism by which gonadal sex determination is controlled in teleosts. Here we show that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh) gene, induces male sex determination in Nile tilapia. amhy is a tandem duplicate located immediately downstream of amhΔ-y on the Y chromosome. The coding sequence of amhy was identical to the X-linked amh (amh) except a missense SNP (C/T) which changes an amino acid (Ser/Leu92) in the N-terminal region...
November 2015: PLoS Genetics
Junjie Wu, Shuting Xiong, Jing Jing, Xin Chen, Weimin Wang, Jian-Fang Gui, Jie Mei
YY super-males have rarely been detected in nature and only been artificially created in some fish species including tilapia and yellow catfish (Pelteobagrusfulvidraco), which provides a promising model for testis development and spermatogenesis. In our previous study, significant differences in morphology and miRNA expression were detected between XY and YY testis of yellow catfish. Here, solexa sequencing technology was further performed to compare mRNA expression between XY and YY testis. Compared with unigenes expressed in XY testis, 1146 and 1235 unigenes have significantly higher and lower expression in YY testis, respectively...
2015: PloS One
Ngo Van Hai
This study aims to present research findings from the use of probiotics in tilapia aquaculture. In omnivorous species of tilapia aquaculture, intestines and gonads, rearing water and sediments or even commercial products, can be sources for acquiring appropriate probiotics. Administration of probiotics varies from direct oral/water routine to feed additives, of which the latter is most commonly used. Probiotic applications can be either mono or multiple strains. Dosage and duration of time are significant factors in providing desired results...
August 2015: Fish & Shellfish Immunology
Chien-Mei Hsiao, Yu-Sheng Wu, Fan-Hua Nan, Shih-Ling Huang, Lynette Chen, Shiu-Nan Chen
The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method...
April 10, 2015: International Wound Journal
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"