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https://www.readbyqxmd.com/read/28507505/protein-protein-interaction-among-the-foxp-family-members-and-their-regulation-of-two-target-genes-vldlr-and-cntnap2-in-the-zebra-finch-song-system
#1
Ezequiel Mendoza, Constance Scharff
The Forkhead transcription factor FOXP2 is implicated in speech perception and production. The avian homolog, FoxP2 contributes to song learning and production in birds. In human cell lines, transcriptional activity of FOXP2 requires homo-dimerization or dimerization with paralogs FOXP1 or FOXP4. Whether FoxP dimerization occurs in the brain is unknown. We recently showed that FoxP1, FoxP2 and FoxP4 (FoxP1/2/4) proteins are co-expressed in neurons of Area X, a song control region in zebra finches. We now report on dimer- and oligomerization of zebra finch FoxPs and how this affects transcription...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28465536/metformin-disrupts-malignant-behavior-of-oral-squamous-cell-carcinoma-via-a-novel-signaling-involving-late-sv40-factor-aurora-a
#2
Chang-Han Chen, Hsin-Ting Tsai, Hui-Ching Chuang, Li-Yen Shiu, Li-Jen Su, Tai-Jan Chiu, Sheng-Dean Luo, Fu-Min Fang, Chao-Cheng Huang, Chih-Yen Chien
Conventional therapeutic processes in patient with OSCC are associated with several unfavorable effects leading to patients with poor survival rate. Metformin has been shown to protect against a variety of specific diseases, including cancer. However, the precise roles and mechanisms underlying the therapeutic effects of metformin on OSCC remain elusive. In the current study, in vitro and xenograft model experiments revealed that metformin inhibited growth and metastasis of oral cancer cells. Importantly, metformin-restrained tumorigenesis of oral cancer was accompanied with strong decrease of both Aurora-A and Late SV40 Factor (LSF) expressions...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28424574/present-molecular-limitations-of-on-bipolar-cell-targeted-gene-therapy
#3
Michiel van Wyk, Elmar C Hulliger, Lara Girod, Andreas Ebneter, Sonja Kleinlogel
Recent studies have demonstrated the safety and efficacy of ocular gene therapy based on adeno-associated viral vectors (AAVs). Accordingly, a surge in promising new gene therapies is entering clinical trials, including the first optogenetic therapy for vision restoration. To date, optogenetic therapies for vision restoration target either the retinal ganglion cells (GCs) or presynaptic ON-bipolar cells (OBCs). Initiating light responses at the level of the OBCs has significant advantages over optogenetic activation of GCs...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28383556/synovial-cell-death-is-regulated-by-tnf-%C3%AE-induced-expression-of-b-cell-activating-factor-through-an-erk-dependent-increase-in-hypoxia-inducible-factor-1%C3%AE
#4
Jae-Wook Lee, Jiyoung Lee, Sung Hee Um, Eun-Yi Moon
B-cell activating factor (BAFF) has a role in the maturation and maintenance of B cells and is associated with rheumatoid arthritis (RA). Here, we investigated whether tumor necrosis factor (TNF)-α-induced BAFF expression controls the survival of fibroblast-like synoviocytes (FLS) and whether their survival can be regulated by TNF-α-mediated upregulation of hypoxia-inducible factor (HIF)-1α using MH7A synovial cells transfected with the SV40 T antigen. More TNF-α-treated cells died compared with the control...
April 6, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28356524/sgta-dependent-regulation-of-hsc70-promotes-cytosol-entry-of-sv40-from-the-er
#5
Allison Dupzyk, Jeffrey M Williams, Parikshit Bagchi, Takamasa Inoue, Billy Tsai
Membrane penetration by non-enveloped viruses remains enigmatic. In the case of the non-enveloped polyomavirus SV40, the virus penetrates the endoplasmic reticulum (ER) membrane to reach the cytosol and then traffics to the nucleus to cause infection. We previously demonstrated that the cytosolic Hsc70-SGTA-Hsp105 complex is tethered to the ER membrane where Hsp105 and SGTA facilitate the extraction of SV40 into the cytosol. We now find that Hsc70 also ejects SV40 into the cytosol in a step regulated by SGTA...
March 29, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28352540/promoter-transgene-and-cell-line-effects-in-the-transfection-of-mammalian-cells-using-pdmaema-based-nano-stars
#6
Alexander Raup, Valérie Jérôme, Ruth Freitag, Christopher V Synatschke, Axel H E Müller
Non-viral transfection protocols are typically optimized using standard cells and reporter proteins, potentially underestimating cellular or transgene effects. Here such effects were studied for two human (Jurkat, HEK-293) and two rodent (CHO-K1, L929) cell lines and three fluorescent reporter proteins. Expression of the enhanced green fluorescent protein (EGFP) was studied under the control of the human elongation factor 1 alpha promoter and three viral promoters (SV40, SV40/enhancer, CMV), that of ZsYellow1 (yellow fluorescence) and mCherry (red fluorescence) for the CMV promoter...
September 2016: Biotechnology Reports
https://www.readbyqxmd.com/read/28216629/matrix-attachment-region-combinations-increase-transgene-expression-in-transfected-chinese-hamster-ovary-cells
#7
Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia, Tian-Yun Wang
Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell expression system. To investigate the effects of MAR combinations on transgene expression and the underlying regulatory mechanisms, we generated constructs in which the enhanced green fluorescent protein (eGFP) gene flanked by different combinations of human β-interferon and β-globin MAR (iMAR and gMAR, respectively), which was driven by the cytomegalovirus (CMV) or simian virus (SV) 40 promoter...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210900/false-responses-of-renilla-luciferase-reporter-control-to-nuclear-receptor-tr4
#8
Dongyun Zhang, Sam S Atlasi, Krishna K Patel, Zihao Zhuang, Anthony P Heaney
Renilla luciferase reporter is a widely used internal control in dual luciferase reporter assay system, where its transcription is driven by a constitutively active promoter. However, the authenticity of the Renilla luciferase response in some experimental settings has recently been questioned. Testicular receptor 4 (TR4, also known as NR2C2) belongs to the subfamily 2 of nuclear receptors. TR4 binds to a direct repeat regulatory element in the promoter of a variety of target genes and plays a key role in tumorigenesis, lipoprotein regulation, and central nervous system development...
February 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28206953/zinc-finger-protein-zfp36l1-promotes-osteoblastic-differentiation-but-represses-adipogenic-differentiation-of-mouse-multipotent-cells
#9
Kuo-Yun Tseng, Yi-Hsuan Chen, Shankung Lin
Zinc finger protein 36, C3H type-like 1 (ZFP36L1) is a member of the tristetraprolin (TTP) family and its role in the aging-related bone loss is currently unknown. We present evidence that ZFP36L1 expression in rat femurs and bone marrow mesenchymal stem cells (bmMSCs) was down-regulated with aging. ZFP36L1 knockdown decreased osteoblastic differentiation of MC3T3-E1 and C3H10T1/2 cells, and increased adipogenic differentiation of 3T3-L1 and C3H10T1/2 cells, whereas ZFP36L1 overexpression did the opposite. The finding that ZFP36L1 overexpression enhanced osteoblastic and repressed adipogenic differentiation was also corroborated by ex vivo experiments...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28099942/bigh3-silencing-increases-retinoblastoma-tumor-growth-in-the-murine-sv40-tag-rb-model
#10
Nathalie Allaman-Pillet, Anne Oberson, Daniel F Schorderet
BIGH3, a secreted protein of the extracellular matrix interacts with collagen and integrins on the cell surface. BIGH3 can have opposing functions in cancer, acting either as tumor suppressor or promoter by enhancing tumor progression and angiogenesis. In the eye, BIGH3 is expressed in the cornea and the retinal pigment epithelium and could impact on the development of retinoblastoma, the most common paediatric intraocular neoplasm. Retinoblastoma initiation requires the inactivation of both alleles of the RB1 tumor suppressor gene in the developing retina and tumor progression involves additional genomic changes...
February 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28052271/establishment-and-characterization-of-an-sv40-large-t-antigen-transduced-porcine-colonic-epithelial-cell-line
#11
Bastian Kaiser, Martina Böttner, Thilo Wedel, Ronald M Brunner, Tom Goldammer, Szilvia Lesko, Gotthold Gäbel, Alexander Gleich, Helga Pfannkuche
Continuous cell lines have become indispensable tools that have enabled investigations into cellular mechanisms by increasing experimental reproducibility and sample availability, and decreasing the use of experimental animals. To facilitate studies of epithelial barrier function of the porcine colon, we aimed to establish an epithelial cell line with an extended replicative capacity. Cells were isolated from the proximal colon of a 3-week-old piglet and transduced using a recombinant retroviral vector construct containing the simian virus 40 large T antigen (SV40 TAg)...
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28012275/emc1-dependent-stabilization-drives-membrane-penetration-of-a-partially-destabilized-non-enveloped-virus
#12
Parikshit Bagchi, Takamasa Inoue, Billy Tsai
Destabilization of a non-enveloped virus generates a membrane transport-competent viral particle. Here we probe polyomavirus SV40 endoplasmic reticulum (ER)-to-cytosol membrane transport, a decisive infection step where destabilization initiates this non-enveloped virus for membrane penetration. We find that a member of the ER membrane protein complex (EMC) called EMC1 promotes SV40 ER membrane transport and infection. Surprisingly, EMC1 does so by using its predicted transmembrane residue D961 to bind to and stabilize the membrane-embedded partially destabilized SV40, thereby preventing premature viral disassembly...
December 24, 2016: ELife
https://www.readbyqxmd.com/read/27987076/hop-expression-is-regulated-by-p53-and-ras-and-characteristic-of-a-cancer-gene-signature
#13
Stacey A Mattison, Gregory L Blatch, Adrienne L Edkins
The Hsp70/Hsp90 organising protein (HOP) is a co-chaperone essential for client protein transfer from Hsp70 to Hsp90 within the Hsp90 chaperone machine. Although HOP is upregulated in various cancers, there is limited information from in vitro studies on how HOP expression is regulated in cancer. The main objective of this study was to identify the HOP promoter and investigate its activity in cancerous cells. Bioinformatic analysis of the -2500 to +16 bp region of the HOP gene identified a large CpG island and a range of putative cis-elements...
March 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27896813/transcriptional-and-post-transcriptional-regulation-of-histone-variant-h2a-z-during-sea-urchin-development
#14
Mihai Hajdu, Jasmine Calle, Andrea Puno, Aminat Haruna, César Arenas-Mena
Histone variant H2A.Z promotes chromatin accessibility at transcriptional regulatory elements and is developmentally regulated in metazoans. We characterize the transcriptional and post-transcriptional regulation of H2A.Z in the purple sea urchin Strongylocentrotus purpuratus. H2A.Z depletion by antisense translation-blocking morpholino oligonucleotides during early development causes developmental collapse, in agreement with its previously demonstrated general role in transcriptional multipotency. During H2A...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27864420/the-landscape-of-viral-expression-reveals-clinically-relevant-viruses-with-potential-capability-of-promoting-malignancy-in-lower-grade-glioma
#15
Zheng Wang, Yajing Hao, Chuanbao Zhang, Zhiliang Wang, Xing Liu, Guanzhang Li, Lihua Sun, Jingshan Liang, Jianjun Luo, Dabiao Zhou, Runsheng Chen, Tao Jiang
Purpose: RNA sequencing (RNA-seq) has recently proved to be effective for revealing novel virus-tumor associations. To get a thorough investigation of virus-glioma associations, we screened viruses in gliomas with RNA-seq data from the Chinese Glioma Genome Atlas project. Experimental Design: In total, 325 samples were enrolled into this study. Reads that failed to map to the human genome were aligned to viral genomes and screened for potential virus-derived transcripts. For quantification, VPKM was calculated according to mapped reads weighted by genome sizes and sequencing depth...
November 18, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27787944/generation-of-immortalized-equine-chondrocytes-with-inducible-sox9-expression-allows-control-of-hypertrophic-differentiation
#16
Saliya Gurusinghe, Bryan Hilbert, Gareth Trope, Lexin Wang, Nadeeka Bandara, Padraig Strappe
Immortalization of chondrocytes enables long term in vitro culture; however, the chondrogenic capacity of transformed cells varies, thus highlighting the need to develop a proliferative and tuneable chondrocyte cell line where hypertrophic differentiation can be controlled. In this study the SV40 large T antigen and human telomerase reverse transcriptase were employed to immortalize pooled equine chondrocytes through lentiviral vector mediated transduction either singly or on combination. Transformed chondrocytes proliferated stably over multiple passages, but resulted in significantly lower expression of chondrocyte specific collagen II mRNA (P < 0...
May 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27630227/the-presumed-polyomavirus-viroporin-vp4-of-simian-virus-40-or-human-bk-polyomavirus-is-not-required-for-viral-progeny-release
#17
Stian Henriksen, Terkel Hansen, Jack-Ansgar Bruun, Christine Hanssen Rinaldo
The minor capsid protein of human BK polyomavirus (BKPyV), VP2, and its N-terminally truncated form, VP3, are both important for viral entry. The closely related simian virus 40 (SV40) reportedly produces an additional truncated form of VP2/3, denoted VP4, apparently functioning as a viroporin promoting progeny release. The VP4 open reading frame is conserved in some polyomaviruses, including BKPyV. In this study, we investigated the role of VP4 in BKPyV replication. By transfecting viral genomes into primary human renal proximal tubule epithelial cells, we demonstrated that unaltered BKPyV and mutants with start codon substitutions in VP4 (VP2M229I and VP2M229A) abolishing putative VP4 production were released at the same level to supernatants...
November 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27617744/enterovirus-a71-dna-launched-infectious-clone-as-a-robust-reverse-genetic-tool
#18
Chee Wah Tan, Han Kang Tee, Michelle Hui Pheng Lee, I-Ching Sam, Yoke Fun Chan
Enterovirus A71 (EV-A71) causes major outbreaks of hand, foot and mouth disease, and is occasionally associated with neurological complications and death in children. Reverse genetics is widely used in the field of virology for functional study of viral genes. For EV-A71, such tools are limited to clones that are transcriptionally controlled by T7/SP6 bacteriophage promoter. This is often time-consuming and expensive. Here, we describe the development of infectious plasmid DNA-based EV-A71 clones, for which EV-A71 genome expression is under transcriptional control by the CMV-intermediate early promoter and SV40 transcriptional-termination signal...
2016: PloS One
https://www.readbyqxmd.com/read/27572655/promoter-optimisation-of-lentiviral-vectors-for-efficient-insulin-gene-expression-in-canine-mesenchymal-stromal-cells-potential-surrogate-beta-cells
#19
Pratigya Gautam, Asha Recino, Robert D Foale, Jing Zhao, Shu Uin Gan, Maja Wallberg, Roy Calne, Andrew M L Lever
BACKGROUND: The lack of an ideal cell type that can be easily acquired, modified to produce insulin, and re-implanted has been a limitation for ex vivo insulin gene therapy. Canine diabetes is currently treated with human insulin and is a good model for human diabetes. Mesenchymal stromal cells (MSCs) are a promising candidate cell type for gene therapy. In the present study, we optimised insulin production using lentiviral transduced canine MSCs (cMSCs), aiming to evaluate their ability for use as surrogate beta cells...
October 2016: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27551315/liver-specific-ng37-overexpression-leads-to-diet-dependent-fatty-liver-disease-accompanied-by-cardiac-dysfunction
#20
Xin Zhou, MengMeng Xu, Liyang Wang, Yulian Mu, Rui Feng, Zhilong Dong, Yuexin Pan, Xunzhang Chen, Yongfeng Liu, Shangen Zheng, Donald D Anthony, Jianjie Ma, Williams B Isaacs, Xuehong Xu
BACKGROUND: Environmental factors are well-known causes of diseases. However, aside from a handful of risk indicators, genes' encoding susceptibility to chronic illnesses and their associated environmental triggers are largely unknown. In this era of increasingly rich diets, such genetic predispositions would be immensely helpful from a public health perspective. The novel transgenic mouse model with liver-specific NG37 overexpression characterized in this article identifies the diet-dependent function of NG37 in the pathogenesis of fatty liver disease and cardiac arrhythmia...
2016: Genes & Nutrition
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