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Conexin 43

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https://www.readbyqxmd.com/read/25459080/characterization-and-cardiac-differentiation-of-chicken-spermatogonial-stem-cells
#1
Nguyen T Luan, Neelesh Sharma, Sung-Woo Kim, Pham T H Ha, Yeong-Ho Hong, Sung-Jong Oh, Dong-Kee Jeong
Spermatogonial stem cells (SSCs), a unique population of germline stem cells in adult testis, have the capability to self-renew and produce daughter cells destined to differentiate into spermatozoa throughout the life of the bird. Chicken SSCs were successfully isolated from testicular cells and subsequent analysis was performed to identify pluripotent cells by investigation with cytochemical reagents including Periodic acid-Schiff (PAS), alkaline phosphatase (AP), and antibodies to germline cell specific (DAZL or VASA) and stage-specific embryonic antigens (Oct4, SSEA1, SSEA3, SSEA4, TRA-1-60, and TRA-1-81)...
December 30, 2014: Animal Reproduction Science
https://www.readbyqxmd.com/read/23632630/hydrogen-sulfide-deficiency-and-diabetic-renal-remodeling-role-of-matrix-metalloproteinase-9
#2
Sourav Kundu, Sathnur B Pushpakumar, Aaron Tyagi, Denise Coley, Utpal Sen
Matrix metalloproteinase-9 (MMP-9) causes adverse remodeling, whereas hydrogen sulfide (H2S) rescues organs in vascular diseases. The involvement of MMP-9 and H2S in diabetic renovascular remodeling is, however, not well characterized. We determined whether MMP-9 regulates H2S generation and whether H2S modulates connexin through N-methyl-d-aspartate receptor (NMDA-R)-mediated pathway in the diabetic kidney. Wild-type (WT, C57BL/6J), diabetic (Akita, C57BL/6J-Ins2(Akita)), MMP-9(-/-) (M9KO), double knockout (DKO) of Akita/MMP-9(-/-) mice and in vitro cell culture were used in our study...
June 15, 2013: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/21544982/-cultivated-oral-epithelial-cells-in-oral-cavity-tissue-losses-treatment-with-autologous-transplantation-case-reports
#3
Bogusława Orzechowska-Wylegała', Dariusz Dobrowolski, Bogumił Wowra, Ewa Obuchowicz, Anna Bielecka
UNLABELLED: The rapid development of bioengineering in recent years enables to search for new therapies involving the use of tissue cultures. The aim of this paper is to study and apply the method of keratinocytes culture of mucous membrane on amniotic membrane to cover the losses in the oral cavity. MATERIAL AND METHODS: In four cases keratinocytes culture on amniotic membrane was applied and transplanted to cover antro-oral fistulas and mucosal and mandibular bone losses...
February 2011: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/8704191/are-there-functional-gap-junctions-or-junctional-hemichannels-in-macrophages
#4
L A Alves, R Coutinho-Silva, P M Persechini, D C Spray, W Savino, A C Campos de Carvalho
The existence of functional gap junctions in migratory cells of the immune system is a controversial issue. In this report, we have focused on one particular cell type, namely the macrophages, because connexin-43, a protein that forms gap junctions, has been described in peritoneal macrophages and a macrophage cell line (J774), by Northern and Western blot analysis. To test whether these cell types expressed functional gap junctions, we assayed dye coupling by intracellular injection of Lucifer Yellow. We observed that nonstimulated macrophages are not coupled among themselves and did not form functional gap junctions with an epithelial cell line, which expresses functional gap junctions formed by connexin-43...
July 1, 1996: Blood
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