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https://www.readbyqxmd.com/read/27806763/-use-of-alveolar-bone-mucosa-periosteal-bone-flap-in-dental-implantation
#1
P Xu, X N Li, Xhy Xu, Y N Cheng, D Yu, L Y Lu
To introduce a method of increasing the width of alveolar bone. The patient, who needed dental implantation and had narrow alveolar bone, was selected. The preparation of mucosa-periosteal bone flap included two surgeries. The first surgery was corticotomy, which made a square cortex cut on narrow alveolar bone region. The second surgery was performed four weeks after the first one, which split the alveolar bone and inserted implants. Artificial bone and/or autologous bone was filled between inner and outer bone plate, and collagen membrane and platelet-rich fibrin membrane were used to cover the wound...
November 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27768215/refixation-allograft-and-adjuvant-cell-therapy-to-treat-nonunion-of-the-femoral-neck-in-a-child
#2
Marina Magnani, Giovanni Trisolino, Stefano Stilli
INTRODUCTION: Idiopathic posttraumatic nonunion is a rare condition in children; the treatment of this complication is still controversial in this peculiar population. CASE REPORT: We report a case of a subcapital type II femoral neck fracture, in a 12-year-old boy, which was initially treated by internal fixation and subsequently went on to a nonunion at the femoral neck, in absence of infection or other disease.1 year after index surgery we performed surgical debridement and refixation with plate and bone allograft, added with autologous bone marrow aspiration concentrate (BMAC) and platelet-rich fibrin (PRF)...
October 15, 2016: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/27563616/regeneration-of-mandibular-ameloblastoma-defect-with-the-help-of-autologous-dental-pulp-stem-cells-and-buccal-pad-of-fat-stromal-vascular-fraction
#3
K Manimaran, Rohini Sharma, S Sankaranarayanan, S Mahendra Perumal
Ameloblastoma is benign odontogenic tumor, which is locally aggressive in behavior. Till date, the treatment of choice is resection and reconstruction using a variety of modalities. Inadequate resection may lead to many complications such as bone deformity and dysfunction. This report is about a 14-year-old male with ameloblastoma treated with autologous dental pulp stem cells (DPSCs) and stromal vascular fraction (SVF) and evidence of bone regeneration. Marsupialization was performed; tooth was extracted and sent for DPSC cultivation...
January 2016: Annals of Maxillofacial Surgery
https://www.readbyqxmd.com/read/27338946/dimensional-changes-of-the-post-extraction-alveolar-ridge-preserved-with-leukocyte-and-platelet-rich-fibrin-a-clinical-pilot-study
#4
Andreas Anwandter, Stephanie Bohmann, Mauricio Nally, Ana B Castro, Marc Quirynen, Nelson Pinto
OBJECTIVES: This clinical trial explored the clinical and radiographic dimensional changes of the alveolar ridge in the first 4 months after tooth extraction in combination with the application of Leukocyte- and Platelet Rich Fibrin (L-PRF). METHODS: Eighteen single rooted maxillary and mandibular sockets were filled with L-PRF without soft tissue closure. Clinical measurements (bone sounding) were performed using a customized acrylic stent and radiographic measurements were accomplished using Cone Beam Computed Tomography (CBCT), immediately after tooth extraction and after 4 months...
September 2016: Journal of Dentistry
https://www.readbyqxmd.com/read/27140443/management-of-a-previously-treated-calcified-and-dilacerated-maxillary-lateral-incisor-a%C3%A2-combined-nonsurgical-surgical-approach-assisted-by-cone-beam-computed-tomography
#5
Pooja Gupta Dudeja, Krishan Kumar Dudeja, Arvind Garg, Dhirendra Srivastava, Shibani Grover
Teeth with calcified canals, dilacerated roots, and associated large periradicular lesions involving both cortical plates pose a challenge to dentists. In addition to the nonsurgical endodontic treatment, such teeth may require surgical intervention with concomitant use of bone grafting materials and barrier techniques. These techniques, when combined with the use of a host modulating agent such as platelet-rich fibrin (PRF), may improve the chances of success. A 26-year-old woman was referred for dental treatment with a recurrence of an intraoral sinus tract 2 months after periradicular surgery in the upper anterior region...
June 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27095909/socket-preservation-by-beta-tri-calcium-phosphate-with-collagen-compared-to-platelet-rich-fibrin-a-clinico-radiographic-study
#6
Swati Das, Rajesh Jhingran, Vivek Kumar Bains, Rohit Madan, Ruchi Srivastava, Iram Rizvi
OBJECTIVES: This study was primarily designed to determine the clinico-radiographic efficacy of platelet-rich fibrin (PRF) and beta-tri-calcium phosphate with collagen (β-TCP-Cl) in preserving extraction sockets. MATERIALS AND METHODS: For Group I (PRF), residual sockets (n = 15) were filled with autologous PRF obtained from patients' blood; and for Group II (β-TCP-Cl), residual sockets (n = 15) were filled with β-TCP-Cl. For the sockets randomly selected for Group II (β-TCP-Cl), the reshaped Resorbable Tissue Replacement cone was inserted into the socket...
April 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/27034749/use-of-leukocyte-and-platelet-rich-fibrin-l-prf-in-periodontally-accelerated-osteogenic-orthodontics-paoo-clinical-effects-on-edema-and-pain
#7
Francisco Munoz, Constanza Jiménez, Daniela Espinoza, Alain Vervelle, Jacques Beugnet, Ziyad Haidar
BACKGROUND: Demand for shorter treatment time is common in orthodontic patients. Periodontally Accelerated Osteogenic Orthodontics (PAOO) is a somewhat new surgical procedure which allows faster tooth movement via combining orthodontic forces with corticotomy and grafting of alveolar bone plates. Leukocyte and Platelet-Rich Fibrin (L-PRF) possess hard- and soft-tissue healing properties. Further, evidence of pain-inhibitory and anti-inflammatory potential is growing. Therefore, this study explores the feasibility, intra- and post-operative effects of using L-PRF in PAOO in terms of post-operative pain, inflammation, infection and post-orthodontic stability...
April 2016: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/26886361/effects-on-fibrinogen-fibrin-and-blood-coagulation-of-proteolytic-extracts-from-fruits-of-pseudananas-macrodontes-bromelia-balansae-and-b-hieronymi-bromeliaceae-in-comparison-with-bromelain
#8
María E Errasti, Anabela Prospitti, Carolina A Viana, Mariana M Gonzalez, Márcio V Ramos, Alejandra E Rotelli, Néstor O Caffini
Extracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. Given the close relationship between hemostasis and inflammation, it is attractive to investigate therapeutic agents capable of modulating both systems. The aim of this work was to study the effect of Pm, Bb, and Bh on fibrin(ogen) and blood coagulation compared with stem bromelain (Bro). Action on fibrinogen was electrophoretically and spectrophotometrically evaluated, fibrinolytic activity was measured both electrophoretically and by the fibrin plate assay, and the effect on blood coagulation was studied by conventional coagulation tests (PT and APPT)...
June 2016: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/26809431/comparative-release-of-growth-factors-from-prp-prf-and-advanced-prf
#9
Eizaburo Kobayashi, Laura Flückiger, Masako Fujioka-Kobayashi, Kosaku Sawada, Anton Sculean, Benoit Schaller, Richard J Miron
OBJECTIVES: The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF). MATERIALS AND METHODS: Eighteen blood samples were collected from six donors (3 samples each for PRP, PRF, and A-PRF). Following preparation, samples were incubated in a plate shaker and assessed for growth factor release at 15 min, 60 min, 8 h, 1 day, 3 days, and 10 days...
January 25, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/26363110/delivery-of-tissue-plasminogen-activator-and-streptokinase-magnetic-nanoparticles-to-target-vascular-diseases
#10
Ateke Tadayon, Reza Jamshidi, Akbar Esmaeili
Thrombolytic therapy for acute myocardial infarction standardly makes use of the medications streptokinase (SK) and tissue plasminogen activator (tPA). In this study, the potential of silica-coated magnetic nanoparticles (SiO2-MNPs) as nanocarriers clinical thrombolytic therapy was investigated. SiO2-MNPs for use in targeted therapeutic delivery of tPA and SK were prepared using a combined technique incorporating controlled precipitation and hydrothermal methods. Response surface methodology (RSM) was employed to evaluate the efficiency of the SiO2-MNPs...
November 10, 2015: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/26006020/fibrin-supports-human-fetal-islet-epithelial-cell-differentiation-via-p70-s6k-and-promotes-vascular-formation-during-transplantation
#11
Matthew Riopel, Jinming Li, Mark Trinder, George F Fellows, Rennian Wang
The human fetal pancreas expresses a variety of extracellular matrix (ECM) binding receptors known as integrins. A provisional ECM protein found in blood clots that can bind to integrin receptors and promote β cell function and survival is fibrin. However, its role in support of human fetal pancreatic cells is unknown. We investigated how fibrin promotes human fetal pancreatic cell differentiation in vitro and in vivo. Human fetal pancreata were collected from 15 to 21 weeks of gestation and collagenase digested...
August 2015: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/24684275/buccal-bone-deficiency-in-fresh-extraction-sockets-a-prospective-single-cohort-study
#12
Antonio Barone, Massimiliano Ricci, Georgios E Romanos, Paolo Tonelli, Fortunato Alfonsi, Ugo Covani
OBJECTIVE: The purpose of this prospective single cohort study was to evaluate the use of xenograft and collagen membranes in treating full or partial buccal bone defects of fresh extraction sockets in the esthetic zone. MATERIALS AND METHODS: Thirty-three patients requiring tooth extraction in the anterior maxillary area and showing a complete or partial buccal bone plate deficiency (more than 2 mm) were consecutively enrolled and treated. Corticocancellous porcine bone and platelet-rich fibrin (PRF) with a collagen membrane were used to graft the extraction sockets, and the membranes were left exposed to the oral cavity with a secondary soft tissue healing...
July 2015: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/23999090/functional-characterization-of-a-slow-and-tight-binding-inhibitor-of-plasmin-isolated-from-russell-s-viper-venom
#13
An-Chun Cheng, Inn-Ho Tsai
BACKGROUND: Snake venoms are rich in Kunitz-type protease inhibitors that may have therapeutic applications. However, apart from trypsin or chymotrypsin inhibition, the functions of most of these inhibitors have not been elucidated. A detailed functional characterization of these inhibitors may lead to valuable drug candidates. METHODS: A Kunitz-type protease inhibitor, named DrKIn-II, was tested for its ability to inhibit plasmin using various approaches such as far western blotting, kinetic analyses, fibrin plate assay and euglobulin clot lysis assay...
January 2014: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/22329757/enhanced-cellular-responses-of-human-bone-marrow-stromal-cells-cultured-on-pretreated-surface-with-allogenic-platelet-rich-plasma
#14
Seung Han Shin, Jeong Joon Yoo, Ha Na Kim, Jinwoo Nam, Hee Joong Kim
The principal objective of this study was to evaluate the effects of surface pretreatment with platelet-rich plasma (PRP) on the cellular functions of human bone marrow stromal cells (hBMSCs). The surfaces of tissue culture plates (TCPs) were pretreated by adding PRP followed by centrifugation to bring platelets closer to the surface, followed by incubation for 60 min at 37°C. Then, hBMSCs were seeded onto TCP and TCP pretreated with PRP (TCP-PRP), followed by culture in osteogenic medium. Cell attachment, proliferation, and osteogenic differentiation were evaluated...
2012: Connective Tissue Research
https://www.readbyqxmd.com/read/22260455/enhanced-cellular-responses-of-human-bone-marrow-stromal-cells-cultured-on-pre-treated-surface-with-allogenic-platelet-rich-plasma
#15
Seung Han Shin, Jeong Joon Yoo, Ha Na Kim, Jinwoo Nam, Hee Joong Kim
Abstract The principal objective of this study was to evaluate the effects of surface pre-treatment with platelet-rich plasma (PRP) on the cellular functions of human bone marrow stromal cells (hBMSCs). The surfaces of tissue culture plates (TCPs) were pre-treated by adding PRP followed by centrifugation to bring platelets closer to the surface, followed by incubation for 60 min at 37°C. Then, hBMSCs were seeded onto TCP and TCP pre-treated with PRP (TCP-PRP), followed by culture in osteogenic medium. Cell attachment, proliferation, and osteogenic differentiation were evaluated...
January 19, 2012: Connective Tissue Research
https://www.readbyqxmd.com/read/22200762/antiplatelet-anticoagulant-and-fibrinolytic-effects-of-litchi-chinensis-sonn-extract
#16
Yoon-Young Sung, Won-Kyung Yang, Ho Kyoung Kim
Litchi chinensis Sonn. (lychee), which belongs to the family of Sapindaceae, is a subtropical evergreen tree that is cultivated throughout Southeast Asia, particularly in China. Litchi chinensis has been reported to have anti-inflammatory, antioxidant and antidiabetic activities. However, the antiplatelet and anticoagulant effects of Litchi chinensis have not been reported previously. In this study, we investigated the effects of a 70% ethanol extract from Litchi chinensis (LCE) on platelet aggregation, coagulation and fibrinolysis...
March 2012: Molecular Medicine Reports
https://www.readbyqxmd.com/read/22120925/the-impact-of-factor-xa-inhibition-on-axial-dependent-arterial-thrombus-formation-triggered-by-a-tissue-factor-rich-surface
#17
Nicholas Pugh, Gavin E Jarvis, Annelize Koch, Kjell S Sakariassen, Bill Davis, Richard W Farndale
This study was designed to assess the effect of Factor Xa antagonists on thrombus formation at various axial positions on a tissue factor rich surface under arterial blood flow conditions. Non-anticoagulated, flowing human blood, drawn directly from an antecubital vein, was perfused over a tissue factor coated cover slip in a parallel-plate perfusion chamber. Thrombus surface coverage, thrombus mean height and fibrin surface coverage were measured at six different axial positions by confocal microscopy. Both thrombus surface coverage and mean height decreased along the cover slip axis whereas the fibrin surface coverage increased...
January 2012: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/21717321/biochemical-analysis-of-a-fibrinolytic-enzyme-purified-from-bacillus-subtilis-strain-a1
#18
Won Sik Yeo, Min Jeong Seo, Min Jeong Kim, Hye Hyeon Lee, Byoung Won Kang, Jeong Uck Park, Yung Hyun Choi, Yong Kee Jeong
A fibrinolytic enzyme from Bacillus subtilis strain Al was purified by chromatographic methods, including DEAE Sephadex A-50 column chromatography and Sephadex G-50 column gel filtration. The purified enzyme consisted of a monomeric subunit and was estimated to be approximately 28 kDa in size by SDS-PAGE. The specific activity of the fibrinolytic enzyme was 1632-fold higher than that of the crude enzyme extract. The fibrinolytic activity of the purified enzyme was approximately 0.62 and 1.33 U/ml in plasminogen-free and plasminogen-rich fibrin plates, respectively...
June 2011: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/21311321/antiplatelet-anticoagulant-and-fibrinolytic-activity-in-vitro-of-extracts-from-selected-fruits-and-vegetables
#19
Constanza Torres-Urrutia, Luis Guzmán, Guillermo Schmeda-Hirschmann, Rodrigo Moore-Carrasco, Marcelo Alarcón, Luís Astudillo, Margarita Gutierrez, Gilda Carrasco, José A Yuri, Eduardo Aranda, Iván Palomo
A diet rich in fruits and vegetables is known to decrease the risk of cardiovascular disease. However, the information regarding the antithrombotic activity (antiplatelet, anticoagulant, and fibrinolytic) of fruits and vegetables is scarce. The aim of this study was to assess the antithrombotic activity of extracts from fruits and vegetables widely consumed in central Chile. The study included samples of 19 fruits and 26 vegetables, representative of the local diet. The extracts prepared from each sample included an aqueous (juice or pressed solubles) and/or methanol-soluble fraction...
April 2011: Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis
https://www.readbyqxmd.com/read/21221943/purification-and-biochemical-characterization-of-a-17-kda-fibrinolytic-enzyme-from-schizophyllum-commune
#20
In Suk Park, Jeong Uck Park, Min Jeong Seo, Min Jeong Kim, Hye Hyeon Lee, Sung Ryeal Kim, Byoung Won Kang, Yung Hyun Choi, Woo Hong Joo, Yong Kee Jeong
A fibrinolytic enzyme of the mushroom, Schizophyllum commune was purified with chromatographic methods, including a DEAE-Sephadex A-50 ion-exchange column and gel filtrations with Sephadex G-75 and Sephadex G-50 columns. The analysis of fibrin-zymography and SDS-PAGE showed that the enzyme was a monomeric subunit that was estimated to be approximately 17 kDa in size. The fibrinolytic activity of the enzyme in plasminogen-rich and plasminogen-free fibrin plates was 1.25 and 0.44 U/ml, respectively. The N-terminal amino acid sequence of the purified enzyme was identified as HYNIXNSWSSFID, which was highly distinguished from known fibrinolytic enzymes...
December 2010: Journal of Microbiology / the Microbiological Society of Korea
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