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Embryo culture

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https://www.readbyqxmd.com/read/28722155/metabolite-availability-as-a-window-to-view-the-early-embryo-microenvironment-in-vivo
#1
REVIEW
Kailun Hu, Yang Yu
A preimplantation embryo exists independent of blood supply, and relies on energy sources from its in vivo environment (e.g. oviduct and uterine fluid) to sustain its development. The embryos can survive in this aqueous environment because it contains amino acids, proteins, lactate, pyruvate, oxygen, glucose, antioxidants, ions, growth factors, hormones, and phospholipids - albeit the concentration of each component varies by species, stage of the estrous cycle, and anatomical location. The dynamic nature of this environment sustains the early development from the one-cell zygote to blastocyst, and is reciprocally influenced by the embryo at each embryonic stage...
July 19, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28719824/supplementation-of-l-carnitine-during-in%C3%A2-vitro-maturation-improves-embryo-development-from-less-competent-bovine-oocytes
#2
Drahomira Knitlova, Pavlina Hulinska, Michal Jeseta, Katerina Hanzalova, Bartosz Kempisty, Marie Machatkova
The present study was designed to define the impact of l-carnitine, supplemented during maturation, on bovine oocytes with different meiotic competence in terms of their IVF outcomes. Meiotically more competent (MMC) and less competent (MLC) oocytes were obtained separately from differently sized follicles at selected phases of folliculogenesis. The oocytes were matured with or without l-carnitine, fertilized and cultured to the blastocyst stage. The oocytes were examined for nuclear maturation, mitochondrial cluster formation, lipid consumption, fertilization and embryo development...
July 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/28718081/blastocyst-utilization-rates-after-continuous-culture-in-two-commercial-single-step-media-a-prospective-randomized-study-with-sibling-oocytes
#3
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, Christos A Venetis, George K Petsas, Basil C Tarlatzis, Tryfon G Lainas
PURPOSE: The aim of this study is to determine whether blastocyst utilization rates are different after continuous culture in two different commercial single-step media. METHODS: This is a paired randomized controlled trial with sibling oocytes conducted in infertility patients, aged ≤40 years with ≥10 oocytes retrieved assigned to blastocyst culture and transfer. Retrieved oocytes were randomly allocated to continuous culture in either Sage one-step medium (Origio) or Continuous Single Culture (CSC) medium (Irvine Scientific) without medium renewal up to day 5 post oocyte retrieval...
July 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28712873/the-effect-of-oil-sands-tailings-pond-sediments-on-embryo-larval-walleye-sander-vitreus
#4
J C Raine, D Turcotte, V Tumber, K M Peru, Z Wang, C Yang, J V Headley, J L Parrott
Walleye (Sander vitreus) are a commercially important North American fish species that inhabit the Athabasca River. This river flows through the Athabasca oil sands where natural sources of bitumen erode from the McMurray formation. Little information is available on responses of walleye embryos to oil sands tailings pond sediments in a laboratory setting. The current study describes the design and implementation of a daily-renewal bioassay to assess the potential effects of tailings pond sediments from the Athabasca oil sands area on walleye development...
July 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28712411/calcium-sensing-receptor-casr-is-involved-in-porcine-in-vitro-fertilisation-and-early-embryo-development
#5
C Liu, Y Liu, K Larsen, Y P Hou, H Callesen
It has been demonstrated that extracellular calcium is necessary in fertilisation and embryo development but the mechanism is still not well understood. The present study mainly focussed on the extracellular calcium effector called the calcium-sensing receptor (CASR) and examined its expression in porcine gametes and embryos and its function during fertilisation and early embryo development. By using reverse transcription polymerase chain reaction, CASR was found to be expressed in porcine oocytes, spermatozoa and embryos at different developmental stages...
July 17, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28712378/treatment-of-allicin-improves-maturation-of-immature-oocytes-and-subsequent-developmental-ability-of-preimplantation-embryos
#6
Sang-Gi Jeong, Seung-Eun Lee, Yun-Gwi Park, Yeo-Jin Son, Min-Young Shin, Eun-Young Kim, Se-Pill Park
Allicin (AL) regulates the cellular redox, proliferation, viability, and cell cycle of different cells against extracellular-derived stress. This study investigated the effects of allicin treatment on porcine oocyte maturation and developmental competence. Porcine oocytes were cultured in medium supplemented with 0 (control), 0.01, 0.1, 1, 10 or 100 μM AL, respectively, during in vitro maturation (IVM). The rate of polar body emission was higher in the 0.1 AL-treated group (74.5% ± 2.3%) than in the control (68...
July 17, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28708500/reversible-meiotic-arrest-of-bovine-oocytes-by-egfr-inhibition-and-follicular-hemisections
#7
Paulo Roberto Antunes da Rosa, Matheus Pedrotti De Cesaro, Andressa Minussi Pereira Dau, Raj Duggavathi, Vilceu Bordignon, Paulo Bayard Dias Gonçalves
The objective of this study was to investigate the effects of inhibiting the epidermal growth factor receptor (EGFR) pathway on meiosis blockage and resumption, mRNA expression of genes involved in oocyte maturation and cumulus expansion, and embryo development. Bovine cumulus-oocyte complexes (COCs) were cultured for 15 h in the presence of the EGFR inhibitor (AG1478) and follicular hemisections (FHS). Most of the oocytes (89.3%) remained at the germinal vesicle (GV) stage when cultured in the presence of FHS and 5 μM AG1478...
May 23, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708489/2-methoxystypandrone-improves-in%C3%A2-vitro-produced-bovine-embryo-quality-through-inhibition-of-ikbkb
#8
Ayman Mesalam, Imran Khan, Kyeong-Lim Lee, Seok-Hwan Song, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
2-Methoxystypandrone (2-MS), a naphthoquinone from Polygonum cuspidatum, has been reported to exhibit antioxidant activities. However, its pharmacological properties have not been fully studied in vitro culture system of oocytes and embryos development. In this study, we examined the effects of 2-MS on bovine blastocyst developmental competency during preimplantation using TUNEL assay, qRT-PCR and immunocytochemistry. Oocytes were treated with 1 μM 2-MS during maturation, followed by in vitro fertilized and the zygotes were cultured...
May 19, 2017: Theriogenology
https://www.readbyqxmd.com/read/28708486/improved-quality-of-porcine-embryos-cultured-with-hyaluronan-due-to-the-modification-of-the-mitochondrial-membrane-potential-and-reactive-oxygen-species-level
#9
Marek Romek, Barbara Gajda, Ewa Krzysztofowicz, Marcin Kucia, Agnieszka Uzarowska, Zdzislaw Smorag
Although considerable progress has been made in pig embryo culture systems, the developmental competence and quality of the produced embryos are still lower than their in vivo-derived counterparts. Because hyaluronan (HA) regulates various cellular processes and possesses antioxidant properties, this glycosaminoglycan seems to be a promising supplement in culture media. However, until now, its beneficial influence on in vitro pig embryo development has been debatable. Hence, we aimed to investigate the effect of 0...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28706297/harmless-effects-of-argon-plasma-on-caudal-fin-regeneration-and-embryogenesis-of-zebrafish-novel-biological-approaches-for-safe-medical-applications-of-bioplasma
#10
Min-Kyung Nam, Goo-Young Kim, Si-Eun Yun, Ja-Young Jang, Yong-Hee Kim, Eun Ha Choi, Seongman Kang, Hyangshuk Rhim
The argon plasma jet (Ar-PJ) is widely used in medical fields such as dermatology and dentistry, and it is considered a promising tool for cancer therapy. However, the in vivo effects of Ar-PJ for medical uses have not yet been investigated, and there are no biological tools to determine the appropriate clinical dosages of Ar-PJ. In this study, we used the caudal fin and embryo of zebrafish as novel in vivo tools to evaluate the biosafety of Ar-PJ. Typically, Ar-PJ is known to induce cell death in two-dimensional (2D) cell culture systems...
July 14, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28705896/bmp-signaling-maintains-a-mesoderm-progenitor-cell-state-in-the-mouse-tailbud
#11
Richa Sharma, Maxwell E R Shafer, Eric Bareke, Mathieu Tremblay, Jacek Majewski, Maxime Bouchard
Caudal somites are generated from a pool of progenitor cells located in the tailbud region. These progenitor cells form the presomitic mesoderm that gradually differentiates into somites under the action of the segmentation clock. The signals responsible for tailbud mesoderm progenitor pool maintenance during axial elongation are still elusive. Here, we show that Bmp signaling is sufficient to activate the entire mesoderm progenitor gene signature in primary cultures of caudal mesoderm cells. Bmp signaling acts through the key regulatory genes Brachyury (T) and Nkx1-2 and contributes to the activation of several other regulators of the mesoderm progenitor gene network...
July 13, 2017: Development
https://www.readbyqxmd.com/read/28702912/the-sugarcane-herbicide-ametryn-induces-oxidative-stress-and-developmental-abnormalities-in-zebrafish-embryos
#12
Monica A M Moura, Rhaul Oliveira, Claudio M Jonsson, Inês Domingues, Amadeu M V M Soares, António J A Nogueira
Ametryn (AMT) is one of the most widely used herbicides in tropical sugarcane crops, the main culture of São Paulo State, Brazil. It is known as a diffuse pollutant, being found in surface water and sediment of water bodies adjacent to the crop fields. In the present study, the toxicity of AMT to zebrafish (Danio rerio) embryos was evaluated using developmental and biochemical endpoints. At the biochemical level, lactate dehydrogenase responded at the lowest concentration tested (4 μg L(-1)) indicating a high demand of energy required to cope with the stress condition...
July 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28701751/secretome-of-in-vitro-cultured-human-embryos-contains-extracellular-vesicles-that-are-uptaken-by-the-maternal-side
#13
Elisa Giacomini, Riccardo Vago, Ana Maria Sanchez, Paola Podini, Natasa Zarovni, Valentina Murdica, Roberta Rizzo, Daria Bortolotti, Massimo Candiani, Paola Viganò
Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28697174/uv-radiation-promotes-melanoma-dissemination-mediated-by-the-sequential-reaction-axis-of-cathepsins-tgf-%C3%AE-1-fap-%C3%AE
#14
Petra Wäster, Kyriakos Orfanidis, Ida Eriksson, Inger Rosdahl, Oliver Seifert, Karin Öllinger
BACKGROUND: Ultraviolet radiation (UVR) is the major risk factor for development of malignant melanoma. Fibroblast activation protein (FAP)-α is a serine protease expressed on the surface of activated fibroblasts, promoting tumour invasion through extracellular matrix (ECM) degradation. The signalling mechanism behind the upregulation of FAP-α is not yet completely revealed. METHODS: Expression of FAP-α was analysed after UVR exposure in in vitro co-culture systems, gene expression arrays and artificial skin constructs...
July 11, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28696244/effect-of-vitrification-on-in-vitro-development-and-imprinted-gene-grb10-in-mouse-embryos
#15
Jianfeng Yao, Lixia Geng, Rongfu Huang, Weilin Peng, Xuan Chen, Xiaohong Jiang, Miao Yu, Ming Li, Yanfang Huang, Xiaoyu Yang
Vitrification of embryos is a routine procedure in IVF (in vitro fertilization) laboratories. In the present study, we aimed to investigate the effect of vitrification on mouse preimplantation embryo development in vitro, and effect on the epigenetic status of imprinted gene Grb10 in mouse embryos. The blastocyst formation rate for vitrified 8-cell embryos was similar to the non-vitrified 8-cell embryos, whereas the blastocyst hatching rate was lower than that of the non-vitrified group. The expression level of Grb10 major-type transcript decreased significantly in vitrified blastocysts compared with non-vitrified and in vivo blastocysts...
September 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28696225/glypican-6-promotes-the-growth-of-developing-long-bones-by-stimulating-hedgehog-signaling
#16
Mariana Capurro, Tomomi Izumikawa, Philippe Suarez, Wen Shi, Marzena Cydzik, Tomoyuki Kaneiwa, Jean Gariepy, Luisa Bonafe, Jorge Filmus
Autosomal-recessive omodysplasia (OMOD1) is a genetic condition characterized by short stature, shortened limbs, and facial dysmorphism. OMOD1 is caused by loss-of-function mutations of glypican 6 (GPC6). In this study, we show that GPC6-null embryos display most of the abnormalities found in OMOD1 patients and that Hedgehog (Hh) signaling is significantly reduced in the long bones of these embryos. The Hh-stimulatory activity of GPC6 was also observed in cultured cells, where this GPC increased the binding of Hh to Patched 1 (Ptc1)...
July 10, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28694259/high-efficiency-non-mosaic-crispr-mediated-knock-in-and-mutations-in-f0-xenopus
#17
Yetki Aslan, Emmanuel Tadjuidje, Aaron M Zorn, Sang-Wook Cha
The revolution in CRISPR-mediated genome editing has enabled the mutation and insertion of virtually any DNA sequence, particularly in cell culture where selection can be used to recover relatively rare homologous recombination events. The efficient use of this technology in animal models still presents a number of challenges including the time to establish mutant lines, mosaic gene editing in founder animals, and low homologous recombination rates. Here we report a method for CRISPR-mediated genome editing in Xenopus oocytes with homology-dependent repair (HDR) that provides efficient non-mosaic targeted insertion of small DNA fragments of 40-50 nucleotides, in 4...
July 10, 2017: Development
https://www.readbyqxmd.com/read/28693953/cryopreservation-of-luciola-praeusta-kiesenwetter-coleoptera-lampyridae-embryos-by-vitrification
#18
Kang Li, Xuanli Chen, Xiaoling Song, Xi Wu, Yi Xian
There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) was used...
July 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28693635/acetylation-and-methylation-profiles-of-h3k27-in-porcine-embryos-cultured-in-vitro
#19
Luciana Simões Rafagnin Marinho, Vitor Braga Rissi, Andressa Guidugli Lindquist, Marcelo Marcondes Seneda, Vilceu Bordignon
Methylation and acetylation of histone H3 at lysine 27 (H3K27) regulate chromatin structure and gene expression during early embryo development. While H3K27 acetylation (H3K27ac) is associated with active gene expression, H3K27 methylation (H3K27me) is linked to transcriptional repression. The aim of this study was to assess the profile of H3K27 acetylation and methylation (mono-, di- and trimethyl) during oocyte maturation and early development in vitro of porcine embryos. Oocytes/embryos were fixed at different developmental stages from germinal vesicle to day 8 blastocysts and submitted to an immunocytochemistry protocol to identify the presence and quantify the immunofluorescence intensity of H3K27ac, H3K27me1, H3K27me2 and H3K27me3...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28693630/developmental-competence-of-dromedary-camel-camelus-dromedarius-oocytes-selected-using-brilliant-cresyl-blue-staining
#20
Mohamed Fathi, Mohamed Ashry, Ali Salama, Magdy R Badr
The objectives of the present studies were to investigate the developmental capacity of dromedary camel oocytes selected by brilliant cresyl blue (BCB) staining and to investigate the expression of select transcripts in germinal vesicle (GV) stage oocytes. These transcripts included BMP15 and GDF9 as important transcripts for folliculogenesis and oocyte development, Zar1 and Mater as maternal transcripts required for embryonic development, Cyclin B1 and CDK1 as cell cycle regulators and Oct4 and STAT3 as transcription factors...
July 11, 2017: Zygote: the Biology of Gametes and Early Embryos
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