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https://www.readbyqxmd.com/read/29331237/time-lapse-algorithms-and-morphological-selection-of-day-5-embryos-for-transfer-a-preclinical-validation-study
#1
Ashleigh Storr, Christos Venetis, Simon Cooke, Suha Kilani, William Ledger
OBJECTIVE: To determine the agreement between published time-lapse algorithms in selecting the best day-5 embryo for transfer, as well as the agreement between these algorithms and embryologists. DESIGN: Prospective study. SETTING: Private in vitro fertilization center. PATIENT(S): Four hundred and twenty-eight embryos from 100 cycles cultured in the EmbryoScope. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Interalgorithm agreement as assessed by the Fleiss kappa coefficient...
January 11, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29329912/identification-and-fate-mapping-of-the-pancreatic-mesenchyme
#2
Jesse Angelo, Kimberly D Tremblay
The murine pancreas buds from the ventral embryonic endoderm at approximately 8.75 dpc and a second pancreas bud emerges from the dorsal endoderm by 9.0 dpc. Although it is clear that secreted signals from adjacent mesoderm-derived sources are required for both the appropriate emergence and further refinement of the pancreatic endoderm, neither the exact signals nor the requisite tissue sources have been defined in mammalian systems. Herein we use DiI fate mapping of cultured murine embryos to identify the embryonic sources of both the early inductive and later condensed pancreatic mesenchyme...
January 9, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29325603/culture-conditions-affect-ca2-release-in-artificially-activated-mouse-and-human-oocytes
#3
Yuechao Lu, Davina Bonte, Minerva Ferrer-Buitrago, Mina Popovic, Jitesh Neupane, Margot Van der Jeught, Luc Leybaert, Petra De Sutter, Björn Heindryckx
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM...
January 12, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29318868/embryo-development-until-blastocyst-stage-with-and-without-renewal-of-single-medium-on-day-3
#4
Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales
OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3)...
January 10, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29317193/an-essential-role-for-neuregulin-4-in-the-growth-and-elaboration-of-developing-neocortical-pyramidal-dendrites
#5
Blanca Paramo, Sean Wyatt, Alun M Davies
Neuregulins, with the exception of neuregulin-4 (NRG4), have been shown to be extensively involved in many aspects of neural development and function and are implicated in several neurological disorders, including schizophrenia, depression and bipolar disorder. Here we provide the first evidence that NRG4 has a crucial function in the developing brain. We show that both the apical and basal dendrites of neocortical pyramidal neurons are markedly stunted in Nrg4-/- neonates in vivo compared with Nrg4+/+ littermates...
January 6, 2018: Experimental Neurology
https://www.readbyqxmd.com/read/29314362/a-small-population-of-resident-limb-bud-mesenchymal-cells-expresses-few-msc-associated-markers-but-the-expression-of-these-markers-is-increased-immediately-after-cell-culture
#6
Jessica Cristina Marín-Llera, Jesús Chimal-Monroy
Skeletal progenitors are derived from resident limb bud mesenchymal cells of the vertebrate embryos. However, it remains poorly understood if they represent stem cells, progenitors or multipotent mesenchymal stromal cells (MSC). Derived-MSC of different adult tissues under in vitro experimental conditions can differentiate into the same cellular lineages that are present in the limb. Here, comparing non-cultured versus cultured mesenchymal limb bud cells, we determined the expression of MSC-associated markers, the in vitro differentiation capacity and their gene expression profile...
January 4, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29313222/establishment-of-an-embryonic-cell-line-from-the-american-cockroach-periplaneta-americana-blattaria-blattidae-and-a-preliminary-study-of-telomerase-activity-changes-during-the-culturing-process
#7
Xin Zhang, Ying Feng, Wei-Feng Ding, Xian Li, Si-Cong Xie
Despite the pest status and medicinal value of the American cockroach Periplaneta americana, few attempts have been made to establish cell lines from this insect owing to the difficulty of culturing Blattarian cells. Here, we describe the establishment of the RIRI-PA1 line from P. americana embryo tissue following primary culture in modified Grace's medium containing 20% fetal bovine serum. RIRI-PA1 was found to primarily consist of attached spindle-shaped and giant cells, which attach themselves to their container...
January 8, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29310712/genome-wide-screening-of-dna-methylation-in-bovine-blastocysts-with-different-kinetics-of-development
#8
Jessica Ispada, Camila Bruna de Lima, Marc-André Sirard, Patrícia Kubo Fontes, Marcelo Fábio Gouveia Nogueira, Kelly Annes, Marcella Pecora Milazzotto
BACKGROUND: The timing of the first cell divisions may predict the developmental potential of an embryo, including its ability to establish pregnancy. Besides differences related to metabolism, stress, and survival, embryos with different speeds of development present distinct patterns of gene expression, mainly related to energy and lipid metabolism. As gene expression is regulated by epigenetic factors, and that includes DNA methylation patterns, in this study we compared the global DNA methylation profile of embryos with different kinetics of development in order to identify general pathways and regions that are most influenced by this phenotype...
January 8, 2018: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29310676/functional-role-of-akt-signaling-in-bovine-early-embryonic-development-potential-link-to-embryotrophic-actions-of-follistatin
#9
Mohamed Ashry, Sandeep K Rajput, Joseph K Folger, Jason G Knott, Nabil A Hemeida, Omaima M Kandil, Refaat S Ragab, George W Smith
BACKGROUND: TGF-β signaling pathways regulate several crucial processes in female reproduction. AKT is a non-SMAD signaling pathway regulated by TGF-β ligands essential for oocyte maturation and early embryonic development in the mouse, but its regulatory role in bovine early embryonic development is not well established. Previously, we demonstrated a stimulatory role for follistatin (a binding protein for specific members of TGF-β superfamily) in early bovine embryonic development...
January 8, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29307054/lethal-and-sublethal-effects-of-the-herbicide-atrazine-in-the-early-stages-of-development-of-physalaemus-gracilis-anura-leptodactylidae
#10
Camila F Rutkoski, Natani Macagnan, Cassiane Kolcenti, Guilherme V Vanzetto, Paola F Sturza, Paulo A Hartmann, Marilia T Hartmann
Water sources used as reproductive sites by crying frog, Physalaemus gracilis, are extensively associated with agroecosystems in which the herbicide atrazine is employed. To evaluate the lethal and sublethal effects of atrazine commercial formulation, acute and chronic toxicity tests were performed in the embryonic phase and the beginning of the larval phase of P. gracilis. Tests were started on stage 19 of Gosner (Herpetologica 16:183-190, 1960) and performed in 24-well cell culture plates. Acute tests had a duration of 96 h with embryo mortality monitoring every 24 h...
January 6, 2018: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29304340/capturing-totipotent-stem-cells
#11
REVIEW
Christopher L Baker, Martin F Pera
Minority subpopulations within embryonic stem cell cultures display an expanded developmental potential similar to that of early embryo blastomeres or the early inner cell mass. The ability to isolate and culture totipotent cells capable of giving rise to the entire conceptus would enhance our capacity to study early embryo development, and might enable more efficient generation of chimeric animals for research and organ production for transplantation. Here we review the biological and molecular characterization of cultured cells with developmental potential similar to totipotent blastomeres, and assess recent progress toward the capture and stabilization of the totipotent state in vitro...
January 4, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29301505/optimising-the-chick-chorioallantoic-membrane-xenograft-model-of-neuroblastoma-for-drug-delivery
#12
Rasha Swadi, Grace Mather, Barry L Pizer, Paul D Losty, Violaine See, Diana Moss
BACKGROUND: Neuroblastoma is a paediatric cancer that despite multimodal therapy still has a poor outcome for many patients with high risk tumours. Retinoic acid (RA) promotes differentiation of some neuroblastoma tumours and cell lines, and is successfully used clinically, supporting the view that differentiation therapy is a promising strategy for treatment of neuroblastoma. To improve treatment of a wider range of tumour types, development and testing of novel differentiation agents is essential...
January 4, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29301092/metabolomic-profiling-of-bovine-oviductal-fluid-across-the-oestrous-cycle-using-proton-nuclear-magnetic-resonance-spectroscopy
#13
Julie Lamy, Julie Gatien, Florine Dubuisson, Lydie Nadal-Desbarats, Pascal Salvetti, Pascal Mermillod, Marie Saint-Dizier
In the present study we tested whether regulation of the metabolome in bovine oviductal fluid depended on the stage of the oestrous cycle, the side relative to ovulation and local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected in the preovulatory, postovulatory, mid- and late luteal phases, from cyclic cows at a local abattoir (18-27 cows per stage and side). The metabolomes were assessed by proton nuclear magnetic resonance spectroscopy (H-NMR). In all, 39 metabolites were identified, among which the amino acid glycine and the energy substrates lactate and myoinositol were the most abundant at all stages...
January 5, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29299323/a-transcriptional-view-on-somatic-embryogenesis
#14
REVIEW
Anneke Horstman, Marian Bemer, Kim Boutilier
Somatic embryogenesis is a form of induced plant cell totipotency where embryos develop from somatic or vegetative cells in the absence of fertilization. Somatic embryogenesis can be induced in vitro by exposing explants to stress or growth regulator treatments. Molecular genetics studies have also shown that ectopic expression of specific embryo- and meristem-expressed transcription factors or loss of certain chromatin-modifying proteins induces spontaneous somatic embryogenesis. We begin this review with a general description of the major developmental events that define plant somatic embryogenesis and then focus on the transcriptional regulation of this process in the model plant Arabidopsis thaliana (arabidopsis)...
August 2017: Regeneration
https://www.readbyqxmd.com/read/29298861/hypersensitive-assessment-of-aryl-hydrocarbon-receptor-transcriptional-activity-using-a-novel-truncated-cyp1a-promoter-in-zebrafish
#15
Juan-Juan Luo, Dong-Sheng Su, Shao-Lin Xie, Yi Liu, Pei Liu, Xiao-Jun Yang, De-Sheng Pei
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant (POP), an unintentional byproduct of various industrial processes, and a human carcinogen. The expression of the cytochrome P450 1A (cyp1a) gene is upregulated in the presence of TCDD through activating the aryl hydrocarbon receptor pathway in a dose-dependent manner. Several essential response elements, including the 8 potential xenobiotic response elements in the cyp1a promoter region, have been identified to be the main functional parts for the response to TCDD...
January 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29291021/circulating-grp78-antibodies-from-ovarian-cancer-patients-a-promising-tool-for-cancer-cell-targeting-drug-delivery-system
#16
Kylie Van Hoesen, Sonia Meynier, Pascale Ribaux, Patrick Petignat, Florence Delie, Marie Cohen
Glucose-regulated protein 78 (GRP78) is a chaperone protein that has a high frequency in tumor cells. Normally it is found in the endoplasmic reticulum to assist in protein folding, but under cellular stress, GRP78 influences proliferative signaling pathways at the cell surface. The increased expression elicits autoantibody production, providing a biomarker of ovarian cancer, as well as other types of cancer. This study aims to determine the epitope recognition of GRP78 autoantibodies isolated from serum of ovarian cancer patients and use the identified antibodies to design new drug delivery systems to specifically target cancer cells...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29288978/expression-and-function-of-muc1-in-uterine-tissues-during-early-pregnancy-in-sheep-after-natural-oestrous-or-artificially-induced-oestrous
#17
Xiangguo Wang, Boyang Zhu, Shanhui Xiong, Xihui Sheng, Xiaolong Qi, Qizhen Huang, Chaolei Chen, Yong Guo, Hemin Ni
Mucin 1 (MUC1), a cell surface glycoprotein, is expressed mainly in the endometrial luminal epithelium (LE) and glandular epithelium (GE) of the endometrium in many mammalian species including mice, rats, pigs, sheep, horses and humans during various stages of a menstrual or oestrous cycle, where it plays an important role in embryo implantation and placentation. However, the expression and function of MUC1 in uterine tissues during early pregnancy in sheep after artificially-induced oestrous is not known. Therefore, we investigated the expression and function of MUC1 in the early pregnant and non-pregnant uterine tissues of sheep with natural oestrous or artificially-induced oestrous on days 10, 12, 14, 16 and 18 of the cycle by in situ hybridization, quantitative real-time polymerase chain reaction, immunohistochemical staining and western blotting methods...
December 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29288822/copper-containing-mesoporous-bioactive-glass-promotes-angiogenesis-in-an-in-vivo-zebrafish-model
#18
Lilian B Romero-Sánchez, Manuel Marí-Beffa, Paloma Carrillo, Miguel Ángel Medina, Aránzazu Díaz-Cuenca
The osteogenic and angiogenic responses of organisms to the ionic products of degradation of bioactive glasses (BGs) are being intensively investigated. The promotion of angiogenesis by copper (Cu) has been known for more than three decades. This element can be incorporated to delivery carriers, such as BGs, and the materials used in biological assays. In this work, Cu-containing mesoporous bioactive glass (MBG) in the SiO2-CaO-P2O5 compositional system was prepared incorporating 5% mol Cu (MBG-5Cu) by replacement of the corresponding amount of Ca...
December 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29285739/sonic-hedgehog-regulation-of-the-neural-precursor-cell-fate-during-chicken-optic-tectum-development
#19
Ciqing Yang, Xiaoying Li, Qiuling Li, Han Li, Liang Qiao, Zhikun Guo, Juntang Lin
During nervous system development, neurons project axons over long distances to reach the appropriate targets for correct neural circuit formation. Sonic hedgehog (Shh) is a secreted protein and plays a key role in regulating vertebrate embryogenesis, especially in central nervous system (CNS) patterning, including neuronal migration and axonal projection in the brain and spinal cord. In the developing ventral midbrain, Shh is sufficient to specify a striped pattern of cell fates. Little is known about the molecular mechanisms underlying the Shh regulation of the neural precursor cell fate during the optic tectum development...
December 28, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29285169/mir-30b-regulates-chondrogenic-differentiation-of-mouse-embryo-derived-stem-cells-by-targeting-sox9
#20
Qingde Wa, Peiheng He, Shuai Huang, Jianwei Zuo, Xing Li, Jinsong Zhu, Song Hong, Guoqing Lv, Dongfeng Cai, Dongliang Xu, Xuenong Zou, Yi Liu
The present study aimed to investigate the mechanisms underlying microRNA (miRNA)-mediated regulation of chondrogenic differentiation. Mouse embryo-derived stem cells C3H10T1/2 were cultured and chondrogenic differentiation was induced using transforming growth factor-β3 (TGF-β3). In addition, miRNA expression profiles were detected via miRNA array analysis, and quantitative polymerase chain reaction was performed to verify the differentially expressed miRNAs. Furthermore, bioinformatics software was used to predict the putative targets and the prediction was validated by dual-luciferase reporter assays and western blot analysis...
December 2017: Experimental and Therapeutic Medicine
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