Alex J Eddins, Riley M Bednar, Subhashis Jana, Abigail H Pung, Lea Mbengi, Kyle Meyer, John J Perona, Richard B Cooley, P Andrew Karplus, Ryan A Mehl
Quantitative labeling of biomolecules is necessary to advance areas of antibody-drug conjugation, super-resolution microscopy imaging of molecules in live cells, and determination of the stoichiometry of protein complexes. Bio-orthogonal labeling to genetically encodable noncanonical amino acids (ncAAs) offers an elegant solution; however, their suboptimal reactivity and stability hinder the utility of this method. Previously, we showed that encoding stable 1,2,4,5-tetrazine (Tet)-containing ncAAs enables rapid, complete conjugation, yet some expression conditions greatly limited the quantitative reactivity of the Tet-protein...
December 4, 2023: Bioconjugate Chemistry