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https://www.readbyqxmd.com/read/29031216/description-of-neoparamoeba-longipodia-n-sp-and-a-new-strain-of-neoparamoeba-aestuarina-page-1970-amoebozoa-dactylopodida-from-deep-sea-habitats
#1
Ekaterina Volkova, Alexander Kudryavtsev
Two strains of lobose amoebae have been isolated from the deep-sea bottom sediments of the Sea of Japan (3.6km deep) and Western Atlantic Ocean (5.1km deep). Amoebae of both strains have a dactylopodial mophotype, intracellular kinetoplastid symbiont (Perkinsela-like organism) and have no microscales on the cell surface. The morphology and molecular data of the Sea of Japan strain allow us to unambiguously identify it as Neoparamoeba aestuarina (Page, 1970). At the same time, the Atlantic strain is described as a new species Neoparamoeba longipodia as it differs from other species of the genus Neoparamoeba in morphology and gene sequence data...
September 21, 2017: European Journal of Protistology
https://www.readbyqxmd.com/read/29031196/anti-edematogenic-and-anti-inflammatory-activity-of-the-essential-oil-from-croton-rhamnifolioides-leaves-and-its-major-constituent-1-8-cineole-eucalyptol
#2
Anita Oliveira Brito Pereira Bezerra Martins, Lindaiane Bezerra Rodrigues, Francisco Rafael Alves Santana Cesário, Maria Rayane Correia de Oliveira, Cicera Datiane Morais Tintino, Fyama Ferreira E Castro, Isabel Sousa Alcântara, Maria Neyze Martins Fernandes, Thaís Rodrigues de Albuquerque, Maria Sanadia Alexandre da Silva, Adriano Antunes de Sousa Araújo, Lucindo José Quintans Júniur, José Galberto Martins da Costa, Irwin Rose Alencar de Menezes, Almir Gonçalves Wanderley
The species Croton rhamnifolioides, belonging to the Croton genus, is known in ethnomedicine as "quebra faca" and is used in the treatment of stomach pain, vomiting and fever. This study aims to evaluate the anti-edematogenic and anti-inflammatory effect of Croton rhamnifolioides leaf essential oil (OEFC) and its major constituent: 1,8-cineole (eucalyptol). The essential oil was extracted from fresh leaves through a hydrodistillation system. The chemical analysis was determined by gas chromatography-mass spectrometry (GC-MS)...
October 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29029339/targeted-enrichment-of-large-gene-families-for-phylogenetic-inference-phylogeny-and-molecular-evolution-of-photosynthesis-genes-in-the-portullugo-clade-caryophyllales
#3
Abigail J Moore, Jurriaan M de Vos, Lillian P Hancock, Eric Goolsby, Erika J Edwards
Hybrid enrichment is an increasingly popular approach for obtaining hundreds of loci for phylogenetic analysis across many taxa quickly and cheaply. The genes targeted for sequencing are typically single-copy loci, which facilitate a more straightforward sequence assembly and homology assignment process. However, this approach limits the inclusion of most genes of functional interest, which often belong to multi-gene families. Here we demonstrate the feasibility of including large gene families in hybrid enrichment protocols for phylogeny reconstruction and subsequent analyses of molecular evolution, using a new set of bait sequences designed for the "portullugo" (Caryophyllales), a moderately sized lineage of flowering plants (∼2200 species) that includes the cacti and harbors many evolutionary transitions to C4 and CAM photosynthesis...
September 25, 2017: Systematic Biology
https://www.readbyqxmd.com/read/29029329/a-new-anthropophilic-species-of-simulium-trichodagmia-diptera-simuliidae-from-amazonia-morphology-chromosomes-and-dna-sequences
#4
Jeane Marcelle Cavalcante Do Nascimento, Neusa Hamada, Vanderly Andrade-Souza, Peter H Adler
The black fly Simulium (Trichodagmia) hirtipupa Lutz (Diptera: Simuliidae) is widely distributed in southern Brazil, with one report from Amapá state in the northern region of Brazilian Amazonia. Morphological comparison of northern and southern populations revealed differences in all life stages, corroborated by chromosomal and molecular analyses, and indicated that the population previously identified as S. hirtipupa from Amapá state represents an undescribed species. This new species is described based on all life stages above the egg, and its chromosomal and molecular divergence from S...
October 2, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29029319/new-records-of-phlebotomine-sand-flies-diptera-psychodidae-from-the-state-of-alagoas-northeast-of-brazil
#5
Moises Thiago de Souza Freitas, Carlos Fernando Rocha Dos Santos, Edilberto Marinho de Andrade, Carlos Brisola Marcondes, Valdir de Queiroz Balbino, Felipe Arley Costa Pessoa
Phlebotomine sand flies are small insects of great medical importance. This study presents new records of phlebotomine sand flies, which augment the list of species, recorded in the state of Alagoas, Brazil. Sand flies were collected using CDC miniature light traps. Collections were made between November 2008 and April 2016, in 47 municipalities within the state of Alagoas. Twenty-seven thousand two-hundred forty-four sand flies were collected, representing 18 species in seven genera. Lutzomyia longipalpis was the predominant species (85...
October 4, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29029254/retrospective-proteomic-analysis-of-serum-after-akhmeta-virus-infection-new-suspect-case-identification-and-insights-into-poxvirus-humoral-immunity
#6
Michael B Townsend, Nadia F Gallardo-Romero, Ekaterine Khmaladze, Neil M Vora, Giorgi Maghlakelidze, Marika Geleishvili, Darin S Carroll, Ginny L Emerson, Mary G Reynolds, P S Satheshkumar
Serologic cross-reactivity, a hallmark of orthopoxvirus (OPXV) infection, makes species-specific diagnosis of infection difficult. In this study, we used a Variola virus (VARV) proteome microarray to characterize and differentiate antibody responses to non-vaccinia OPXV infections from smallpox vaccination. The profile of two-case patients infected with newly discovered OPXV, Akhmeta virus (AKMV), exhibited antibody responses of greater intensity and broader recognition of viral proteins and includes the B21/22 family glycoproteins not encoded by vaccinia virus (VACV) strains used as vaccines...
October 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29029231/analysis-of-phylogenomic-tree-space-resolves-relationships-among-marsupial-families
#7
David A Duchêne, Jason G Bragg, Sebastián Duchêne, Linda E Neaves, Sally Potter, Craig Moritz, Rebecca N Johnson, Simon Y W Ho, Mark D B Eldridge
A fundamental challenge in resolving evolutionary relationships across the Tree of Life is to account for heterogeneity in the evolutionary signal across loci. Studies of marsupial mammals have demonstrated that this heterogeneity can be substantial, leaving considerable uncertainty in the evolutionary timescale and relationships within the group. Using simulations and a new phylogenomic data set comprising nucleotide sequences of 1550 loci from 18 of the 22 extant marsupial families, we demonstrate the power of a method for identifying clusters of loci that support different phylogenetic trees...
September 22, 2017: Systematic Biology
https://www.readbyqxmd.com/read/29029067/pharmacodynamics-of-fosfomycin-against-esbl-and-or-carbapenemase-producing-enterobacteriaceae
#8
Fiona Fransen, Kelly Hermans, Maria J B Melchers, Claudia C M Lagarde, Joseph Meletiadis, Johan W Mouton
Background: The increase in antibiotic resistance in Gram-negative bacteria and the limited therapeutic options due to the shortage of new antibiotics have increased the interest of the 'old' antibiotic fosfomycin in the treatment of infections. However, there are contradictory reports on the pharmacodynamics of and emergence of resistance to fosfomycin. Methods: Time-kill assays were performed with 11 ESBL-positive and 3 ESBL-negative strains, exposing the bacteria to 2-fold static concentrations from 0...
October 4, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29029031/impact-of-field-densities-of-the-naturalized-defoliator-caloptilia-triadicae-lepidoptera-gracillariidae-on-the-invasive-weed-chinese-tallowtree
#9
G S Wheeler, S D Hight, S A Wright
Chinese tallowtree, Triadica sebifera (L.) Small (Euphoriaceae), is one of the most aggressive weeds of coastal wetlands and forests of the southeastern United States. The lack of specialist herbivores in the invaded range may be one of the factors that contribute to the invasive nature of this weed. Chinese tallowtree has been the target of a classical biological control project since 2006. Several herbivore species are being tested for biological control of Chinese tallowtree. Concurrently, an adventive herbivore, Caloptilia triadicae Davis (Lepidoptera: Gracillariidae), was found feeding on Chinese tallowtree in the southeastern United States in 2004 and now occurs throughout the invaded range...
September 23, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/29028667/impact-of-antiretroviral-drugs-on-the-microbiome-unknown-answers-to-important-questions
#10
Sandra Pinto-Cardoso, Nichole R Klatt, Gustavo Reyes-Terán
PURPOSE OF REVIEW: Little is known on how different antiretroviral (ARV) drugs affect the gut microbiome in HIV infection; and conflicting data exists on the effect of ARV drugs on residual inflammation/immune activation and microbial translocation. RECENT FINDINGS: Gut microbiome involvement in the transmission and pathogenesis of HIV infection is increasingly being recognized. Various studies have shown that antiretroviral therapy (ART) is unable to restore gut health despite effective suppression of plasma HIV viremia...
October 11, 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/29028617/a-plasma-lipidomics-strategy-reveals-perturbed-lipid-metabolic-pathways-and-potential-lipid-biomarkers-of-human-colorectal-cancer
#11
Sensen Shen, Li Yang, Linnan Li, Yu Bai, Chun Cai, Huwei Liu
To explore underlying molecular mechanisms and identify novel lipid biomarkers promising for colorectal cancer (CRC) diagnosis, a continuous-flow two dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF/MS) method was employed to comprehensively measure lipid species in human plasma of CRC patients and healthy controls. With a total of 427 annotated lipid species, we identified 64 lipid species with corrected p value less than 0.05 and fold change more than 1.5. These significantly altered lipid species were mainly involved in glycerolipids and glycerophospholipids metabolism and sphingolipids metabolism...
October 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29028580/revision-of-the-genus-micromonas-manton-et-parke-chlorophyta-mamiellophyceae-of-the-type-species-m-pusilla-butcher-manton-parke-and-of-the-species-m-commoda-van-baren-bachy-and-worden-and-description-of-two-new-species-based-on-the-genetic-and-phenotypic-characterization
#12
Nathalie Simon, Elodie Foulon, Daphné Grulois, Christophe Six, Yves Desdevises, Marie Latimier, Florence Le Gall, Margot Tragin, Aude Houdan, Evelyne Derelle, Fabien Jouenne, Dominique Marie, Sophie Le Panse, Daniel Vaulot, Birger Marin
The green picoalgal genus Micromonas is broadly distributed in estuaries, coastal marine habitats and open oceans, from the equator to the poles. Phylogenetic, ecological and genomic analyses of culture strains and natural populations have suggested that this cosmopolitan genus is composed of several cryptic species corresponding to genetic lineages. We performed a detailed analysis of variations in morphology, pigment content, and sequences of the nuclear-encoded small-subunit rRNA gene and the second internal transcribed spacer (ITS2) from strains isolated worldwide...
September 14, 2017: Protist
https://www.readbyqxmd.com/read/29028546/enzymatic-characterization-and-crystal-structure-analysis-of-chlamydomonas-reinhardtii-dehydroascorbate-reductase-and-their-implications-for-oxidative-stress
#13
Hsin-Yang Chang, Shu-Tseng Lin, Tzu-Ping Ko, Shu-Mei Wu, Tsen-Hung Lin, Yu-Ching Chang, Kai-Fa Huang, Tse-Min Lee
Dehydroascorbate reductase (DHAR) is a key enzyme for glutathione (GSH)-dependent reduction of dehydroascorbate (DHA) to recycled ascorbate (AsA) in plants, and plays a major role against the toxicity of reactive oxygen species (ROS). Previously, we proposed that the increase of AsA regeneration via enhanced DHAR activity modulates the ascorbate-glutathione cycle activity against photooxidative stress in Chlamydomonas reinhardtii. In the present work, we use site-directed mutagenesis and crystal structure analysis to elucidate the molecular basis of how C...
October 3, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29028069/acclimatization-patterns-in-tropical-reptiles-uncoupling-temperature-and-energetics
#14
Wiebke Berg, Ole Theisinger, Kathrin H Dausmann
The physiological compensation of animals in changing environments through acclimatization has long been considered to be of minor importance in tropical ectotherms due to more stable climatic conditions compared to temperate regions. Contrasting this assumption are reports about a range of metabolic adjustments in tropical species, especially during the last two decades from field acclimatized animals. Metabolic rates are strongly linked to temperature in ectotherms but they also reflect energetic requirements and restrictions...
October 13, 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/29028005/microbiome-and-infectivity-studies-reveal-complex-polyspecies-tree-disease-in-acute-oak-decline
#15
Sandra Denman, James Doonan, Emma Ransom-Jones, Martin Broberg, Sarah Plummer, Susan Kirk, Kelly Scarlett, Andrew R Griffiths, Maciej Kaczmarek, Jack Forster, Andrew Peace, Peter N Golyshin, Francis Hassard, Nathan Brown, John G Kenny, James E McDonald
Decline-diseases are complex and becoming increasingly problematic to tree health globally. Acute Oak Decline (AOD) is characterized by necrotic stem lesions and galleries of the bark-boring beetle, Agrilus biguttatus, and represents a serious threat to oak. Although multiple novel bacterial species and Agrilus galleries are associated with AOD lesions, the causative agent(s) are unknown. The AOD pathosystem therefore provides an ideal model for a systems-based research approach to address our hypothesis that AOD lesions are caused by a polymicrobial complex...
October 13, 2017: ISME Journal
https://www.readbyqxmd.com/read/29027997/microdiversification-in-genome-streamlined-ubiquitous-freshwater-actinobacteria
#16
Stefan M Neuenschwander, Rohit Ghai, Jakob Pernthaler, Michaela M Salcher
Actinobacteria of the acI lineage are the most abundant microbes in freshwater systems, but there are so far no pure living cultures of these organisms, possibly because of metabolic dependencies on other microbes. This, in turn, has hampered an in-depth assessment of the genomic basis for their success in the environment. Here we present genomes from 16 axenic cultures of acI Actinobacteria. The isolates were not only of minute cell size, but also among the most streamlined free-living microbes, with extremely small genome sizes (1...
October 13, 2017: ISME Journal
https://www.readbyqxmd.com/read/29027722/the-coordination-and-supramolecular-chemistry-of-gold-metalloligands
#17
Juan Gil-Rubio, José Vicente
This review article deals with the use of gold metalloligands as building blocks for the assembly of heterometallic complexes. Several families of gold complexes decorated with crown-ether, amide, pyridine, bipyridine, terpyridine, carboxylato, aminoacid or pi-alkyne binding sites have been reported. Adducts of these metalloligands with alkaline or transition metal cations, or transition metal or lanthanide complexes, have been isolated and structurally characterized. The reported heterometallic species range from simple dinuclear complexes to self-assembled supramolecules, coordination polymers or solids...
October 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29027707/a-trichosporonales-genome-tree-based-on-27-haploid-and-three-evolutionarily-conserved-natural-hybrid-genomes
#18
Masako Takashima, Sira Sriswasdi, Ri-Ichiroh Manabe, Moriya Ohkuma, Takashi Sugita, Wataru Iwasaki
To construct a backbone tree consisting of basidiomycetous yeasts, draft genome sequences from 25 species of Trichosporonales (Tremellomycetes, Basidiomycota) were generated. In addition to the hybrid genomes of Trichosporon coremiiforme and T. ovoides that we described previously, we identified an interspecies hybrid genome in Cutaneotrichosporon mucoides (formerly T. mucoides). This hybrid genome had a gene retention rate of approximately 55%, and its closest haploid relative was C. dermatis. After constructing the C...
October 13, 2017: Yeast
https://www.readbyqxmd.com/read/29027651/syndesmis-aethopharynx-westervelt-kozloff-1990-rhabdocoela-umagillidae-a-revisitation-supported-by-scanning-electron-microscopy-and-molecular-analyses
#19
Francisca I Cavaleiro, Luís F Rangel, Duarte G Frade, Maria J Santos
Species of Syndesmis Francois, 1886 are rhabdocoel platyhelminths typically found in echinoids. Our knowledge of this group is based on old and insufficient studies, generally representing light microscopy-based species descriptions. Syndesmis aethopharynx Westervelt & Kozloff, 1990 is an understudied endosymbiont of Paracentrotus lividus (Lamarck), which is likely to have been confused with the type-species, i.e. Syndesmis echinorum François, 1886, in the literature. In this work, S. aethopharynx is revisited based on new data on surface morphology and phylogeny and basic ecological data are provided...
October 13, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/29027592/a-novel-citrus-viroid-found-in-australia-tentatively-named-citrus-viroid-vii
#20
G A Chambers, N J Donovan, S Bodaghi, S M Jelinek, G Vidalakis
A novel citrus viroid was discovered in a non-symptomatic Lisbon lemon (Citrus x limon L. Burm.f.) tree in New South Wales, Australia. Bioindexing, molecular detection and characterization involving sequencing combined with in silico analysis for the identification of the viroid-RNA hallmark properties of transmissibility and autonomous replication as well as specific sequence and structural motifs suggest that this viroid is a member of a new species in the genus Apscaviroid, family Pospiviroidae, which we have tentatively named "citrus viroid VII" (CVd-VII)...
October 13, 2017: Archives of Virology
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