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Nitric Oxide

Michelle Bedenbaugh, Rick McCosh, Justin Lopez, John Connors, Robert L Goodman, Stan Hileman
<br> Background: Neuronal intermediates that communicate estrogen and progesterone feedback to gonadotropin-releasing hormone (GnRH) neurons are essential for modulating reproductive cyclicity. Individually, kisspeptin and nitric oxide (NO) influence GnRH secretion. However, it is possible these two neuronal intermediates interact with one another to affect reproductive cyclicity. METHODS: We investigated the neuroanatomical relationship of one isoform of the enzyme that synthesizes NO, neuronal nitric oxide synthase (nNOS), to kisspeptin and GnRH in adult female rhesus monkeys and sheep using dual-label immunofluorescence...
June 21, 2018: Neuroendocrinology
Marcus C Parrish, Isaac A Chaim, Zachary D Nagel, Steven R Tannenbaum, Leona D Samson, Bevin P Engelward
It is well established that inflammation leads to the creation of potent DNA damaging chemicals, including reactive oxygen and nitrogen species. Nitric oxide can react with glutathione to create S-nitrosoglutathione (GSNO), which can in turn lead to S-nitrosated proteins. Of particular interest is the impact of GSNO on the function of DNA repair enzymes. The base excision repair (BER) pathway can be initiated by the alkyl-adenine DNA glycosylase (AAG), a monofunctional glycosylase that removes methylated bases...
May 5, 2018: DNA Repair
Xiaoong Liu, Xin Xu, Ruru Shang, Yingjie Chen
Patients with chronic kidney disease have an increased cardiovascular morbidity and mortality. It has been recognized that the traditional cardiovascular risk factors could only partially explain the increased cardiovascular morbidity and mortality in patients with chronic kidney disease. Asymmetric dimethylarginine (ADMA) and N-monomethy l-arginine (L-NMMA) are endogenous inhibitors of nitric oxide synthases that attenuate nitric oxide production and enhance reactive oxidative specie generation. Increased plasma ADMA and/or L-NMMA are strong and independent risk factor for chronic kidney disease, and various cardiovascular diseases such as hypertension, coronary artery disease, atherosclerosis, diabetes, and heart failure...
June 18, 2018: Nitric Oxide: Biology and Chemistry
Yves Boucher, Nathan Moreau, Annie Mauborgne, Wisam Dieb
We explored the molecular and behavioral effects of a perineural Lipopolysaccharide (LPS)-mediated inflammatory priming on the development and maintenance of painful post-traumatic trigeminal neuropathy (PPTTN) following infra-orbital nerve chronic constriction injury (CCI-IoN) in rats. Rats were pretreated with repetitive perineural injections in the vicinity of the IoN of either LPS or vehicle (Vhcl) before being submitted to CCI-IoN. Orofacial pain-like behaviors (response to Von Frey Filament testing and spontaneous isolated face grooming) were measured during the period of LPS injections (three weeks) and following CCI-IoN surgery (two weeks)...
June 18, 2018: Physiology & Behavior
Seu-Hwa Chen, June-Horng Lue, Yung-Jung Hsiao, Shu-Mei Lai, Hsin-Ying Wang, Chi-Te Lin, Ya-Chin Chen, Yi-Ju Tsai
In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the cuneate nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI...
2018: PloS One
Martin Wepler, Tamara Merz, Ulrich Wachter, Josef Vogt, Enrico Calzia, Angelika Scheuerle, Peter Möller, Michael Gröger, Sandra Kress, Marina Fink, Britta Lukaschewski, Grégoire Rumm, Bettina Stahl, Michael Georgieff, Markus Huber-Lang, Roberta Torregrossa, Matthew Whiteman, Oscar McCook, Peter Radermacher, Clair Hartmann
Hemorrhagic shock (HS) accounts for 30-40% of trauma-induced mortality, which is due to multi-organ-failure subsequent to systemic hyper-inflammation, triggered by hypoxemia and tissue ischemia. The slow-releasing, mitochondria-targeted H2S donor AP39 exerted beneficial effects in several models of I/R-injury and acute inflammation. Therefore, we tested the effects of AP39-treatment in a murine model of combined blunt chest trauma (TxT) and HS with subsequent resuscitation. METHODS: After blast wave-induced TxT or sham procedure, anesthetized and instrumented mice underwent 1 hour of hemorrhage followed by 4 hours of resuscitation comprising an i...
June 20, 2018: Shock
Jose Sergio Possomato-Vieira, Jessica Sabbatine Chimini, Maria Luiza Santos da Silva, Carlos A Dias-Junior
Dysregulation of hydrogen sulphide (H2 S) producing enzymes has been related to hypertensive pregnancy and H2 S donor, sodium hydrosulphide (NaHS) exerts antihypertensive effects, modulates angiogenic factors production and acts as an antioxidant. Moreover, reduction in nitric oxide (NO) bioavailability is related to hypertensive pregnancy and H2 S may interact with NO, modulating its production. We aimed to investigate the NaHS effects in hypertension-in-pregnancy and also in feto-placental parameters. Female Wistar rats (200-250 g) were mated and desoxycorticosterone acetate injections followed by replacement of water by 0...
June 21, 2018: Clinical and Experimental Pharmacology & Physiology
Alejandro Marín Medina, Eduardo Esteban Zubero, Moisés Alejandro Alatorre Jiménez, Sara Anabel Alonso Barragan, Carlos Arturo López García, José Juan Gómez Ramos, Juan Francisco Santoscoy Gutierrez, Zurisadai González Castillo
Chronic kidney disease (CKD) is a multifactorial pathophysiologic irreversible process that often leads to a terminal state in which the patient requires renal replacement therapy. Most cases of CKD are due to chronic-degenerative diseases and endothelial dysfunction is one of the factors that contribute to its pathophysiology. One of the most important mechanisms for proper functioning of the endothelium is the regulation of the synthesis of nitric oxide. This compound is synthesized by the enzyme nitric oxide synthase, which has 3 isoforms...
May 28, 2018: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
Qijie Liu, Chao Zhang, Yanling Bao, Guangze Dai
Optimizing supports for microorganisms is required for bioreactors. Carbon fibers (CF) were employed as supports for microorganisms. To optimize CF supports for immobilizing bacterial cells, we used methods of nitric acid oxidation and calcium ion coverage. We evaluated the capacity of these CF supports (untreated CF, nitric acid oxidation CF and Ca2+ -covered CF) via bacterial cell adhesion tests, based on extended Derjaguin -Landau -Verwey -Overbeek (XDLVO) theory. The results implied that because of the high hamaker constants, oxidized CF supports had higher capacity in this regard than untreated CF supports...
June 21, 2018: Environmental Technology
Yue Gu, Li-Ping Xie, Guo-Liang Meng, Yong Ji
Diabetes and its complications continue to be an increasing global health problem. Hydrogen sulfide (H2S)has emerged as the third gasotransmitter following the nitric oxide and carbon monoxide in the body, and plays an important role in both physiology and pathophysiology. Recent work has suggested important roles for H2S in the regulation of pancreatic β-cell function, as well as development of insulin resistance and diabetic complications. Targeting H2S represents a novel therapeutic approach in diabetes and its associated complications...
June 21, 2018: Sheng Li Ke Xue Jin Zhan [Progress in Physiology]
Ye-Yi Li, Man Shang, Kun-Wei Zhang, Su Wei, Chao Liu, Qian Zhu, Jun-Yu Zhao, Yan-Na Wu, Jun-Qiu Song, Yan-Xia Liu
OBJECTIVES: To investigate the effects of Astragaloside IV (AST) on diastolic function of rat thoracic aorta rings which was injured by microvesicles derived from hypoxia/reoxygenation (H/R)-treated human umbilical vein endothelial cells (HUVECs), and the mechanism of AST. METHODS: H/R-induced endothelial microvesicles (H/R-EMVs) were generated from cultured HUVECs in vitro under the condition of hypoxia for 12 hour/Reoxygenation for 4 hour, H/R-EMVs were stored in D-Hank's solution...
February 8, 2018: Chinese Journal of Applied Physiology
Parimala Narne, Vimal Pandey, Prakash Babu Phanithi
Nitric oxide (NO) and hydrogen sulfide (H2 S) are the key gasotransmitters with an imperious role in the maintenance of cerebrovascular homeostasis. A decline in their levels contributes to endothelial dysfunction that portends ischemic stroke (IS) or cerebral ischemia/reperfusion (CI/R). Nevertheless, their exorbitant production during CI/R is associated with exacerbation of cerebrovascular injury in the post-stroke epoch. NO-producing nitric oxide synthases are implicated in IS pathology and their activity is regulated, inter alia, by various post-translational modifications and chromatin-based mechanisms...
June 20, 2018: Molecular Neurobiology
La-Mei Wang, Na Tang, Hua Zhong, Li-Juan Pang, Chun-Jun Zhang, Fang He
The present study was to investigate the role of the interaction between canonical transient receptor potential channel 1 (TRPC1) and calcium release-activated calcium modulator 1 (Orai1) in extracellular Ca2+ -sensing receptor (CaR)-induced extracellular Ca2+ influx and nitric oxide (NO) production. Human umbilical vein endothelial cells (HUVECs) were incubated with CaR agonist Spermine [activating store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC)] alone or in combination with the following reagents: CaR negative allosteric modulator Calhex231 plus ROC analogue TPA (activating ROC and blocking SOC), Ro31-8220 (PKC inhibitor that activates SOC and blocks ROC) or Go6967 (PKCs and PKCµ inhibitor that activates SOC and blocks ROC)...
June 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
B Yao, D W Liu, W Z Chai, X T Wang, H M Zhang
Objective: To study the effect of N-acetyl-L-cysteine (NAC) on vascular heterogeneity in microcirculation dysfunction caused by endotoxemia and its mechanism. Methods: Eighteen Japanese big ear rabbits were divided into three groups: Control group, Lipopolysaccharides (LPS) group and LPS+ NAC group.A dose of 2 mg/kg LPS was injected to set up endotoxemia rabbit models.Rabbits in the LPS+ NAC group were infused with NAC at a dose of 350 mg/kg, and the same amount of normal saline were infected intravenously to the rabbits in the Control and LPS group...
June 19, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Priscila Ferreira Batista, Alan Carlos Costa, Caroline Müller, Robson de Oliveira Silva-Filho, Fábia Barbosa da Silva, Andrew Merchant, Giselle Camargo Mendes, Kelly Juliane Telles Nascimento
Crambe abyssinica is widely cultivated in the off-season in the Midwest region of Brazil with great potential for biodeisel production. Low precipitation is characteristic of this region, which can drastically affect the productivity of C. abyssinica. Signaling molecules, such as nitric oxide (NO), can potentially alleviate the effects of water stress on plants. Here we test whether nitric oxide, applied by donor sodium nitroprusside (SNP), can alleviate the occurrence of water deficit damages in Crambe plants and maintain physiological and biochemical processes...
June 12, 2018: Plant Physiology and Biochemistry: PPB
Kung-Ha Choi, Mi Hwa Park, Hyun Ah Lee, Ji-Sook Han
Exposure to high levels of glucose may cause glucotoxicity, leading to pancreatic β cell dysfunction, including cell apoptosis and impaired glucose-stimulated insulin secretion. The aim of this study was to explore the effect of cyanidin-3-rutinoside (C3R), a derivative of anthocyanin, on glucotoxicity-induced apoptosis in INS-1 pancreatic β cells. Glucose (30 mM) treatment induced INS-1 pancreatic β cell death, but glucotoxicity and apoptosis significantly decreased in cells treated with 50 μM C3R compared to that observed in 30 mM glucose-treated cells...
June 20, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Hai-Jun Hu, Yue Zhou, Zhu-Zhen Han, Yan-Hong Shi, Shu-Sheng Zhang, Zheng-Tao Wang, Li Yang
Twelve new abietane diterpenoids (1-12) and 31 known analogues (13-43) were isolated from a medicinal Chinese herb, Clerodendrum trichotomum Thunberg. The absolute configurations of 1-3 were established on the basis of ECD and X-ray crystallography data, whereas that of 4 was elucidated by comparison of experimental and calculated ECD data. Eight diterpenoids, 15,16-dehydroteuvincenone G (1), trichotomin A (4), 2α-hydrocaryopincaolide F (7), villosin C (20), 15-dehydro-17-hydroxycyrtophyllone A (22), demethylcryptojaponol (38), 6β-hydroxydemethylcryptojaponol (39), and trichotomone (43), exerted inhibitory effects against NO production with IC50 values of 5...
June 20, 2018: Journal of Natural Products
Rodolfo Sumayao, Philip Newsholme, Tara McMorrow
Nitric oxide (NO) has been shown to play an important role in renal physiology and pathophysiology partly through its influence on various transport systems in the kidney proximal tubule. The role of NO in the kidney dysfunction associated with the lysosomal storage disorder, cystinosis, is largely unknown. In the present study, the effects of iNOS-specific inhibitor, 1400W, on Na+ ,K+ -ATPase activity and expression, mitochondrial integrity and function, nutrient metabolism, and apoptosis were investigated in Ctns null proximal tubular epithelial cells (PTECs)...
June 20, 2018: Clinical and Experimental Pharmacology & Physiology
Zsófia Lázár, Péter Horváth, Rita Puskás, Gabriella Gálffy, György Losonczy, Ildikó Horváth, András Bikov
OBJECTIVE: Extended nitric oxide (NO) analysis offers the partitioned monitoring of inflammation in central and peripheral airways. Different mathematical models are used to estimate pulmonary NO dynamics in asthma with variable results and limitations. We aimed to establish a protocol for extended NO analysis in patients with differing asthma severity. METHODS: Forty patients with stable asthma and twenty-five matched control subjects were recruited. Exhaled NO was measured at constant flow rates between 10 and 300 mL/s...
June 20, 2018: Journal of Asthma: Official Journal of the Association for the Care of Asthma
Dario Kringel, Catharina Lippmann, Michael J Parnham, Eija Kalso, Alfred Ultsch, Jörn Lötsch
BACKGROUND: Human genetic research has implicated functional variants of more than one hundred genes in the modulation of persisting pain. Artificial intelligence and machine learning techniques may combine this knowledge with results of genetic research gathered in any context, which permits the identification of the key biological processes involved in chronic sensitization to pain. METHODS: Based on published evidence, a set of 110 genes carrying variants reported to be associated with modulation of the clinical phenotype of persisting pain in eight different clinical settings was submitted to unsupervised machine-learning aimed at functional clustering...
June 19, 2018: European Journal of Pain: EJP
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