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https://www.readbyqxmd.com/read/28629427/targeting-vegfr-3-2-signaling-pathways-with-ad0157-a-potential-strategy-against-tumor-associated-lymphangiogenesis-and-lymphatic-metastases
#1
Melissa García-Caballero, Jenny Paupert, Silvia Blacher, Maureen Van de Velde, Ana Rodríguez Quesada, Miguel Angel Medina, Agnès Noël
BACKGROUND: Lymphatic metastasis is one of the leading causes of death in patients with different types of cancer and is the main prognostic factor for the disease survival. The formation of new lymphatic vessels (lymphangiogenesis) in primary tumors facilitates tumor cell dissemination to regional lymph nodes and correlates with distant metastases. Lymphangiogenesis has thus emerged as a suitable therapeutic target to block metastases, but no anti-lymphangiogenic compounds have been approved for clinical use to date...
June 19, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28627621/identification-of-different-macrophage-subpopulations-with-distinct-activities-in-a-mouse-model-of-oxygen-induced-retinopathy
#2
Yanji Zhu, Ling Zhang, Qing Lu, Yushuo Gao, Yujuan Cai, Ailing Sui, Ting Su, Xi Shen, Bing Xie
The aim of the present study was to characterize the phenotypic shift, quantity and role changes in different subgroups of retinal macrophages in a mouse model of oxygen-induced retinopathy (OIR). The mRNA expression levels of macrophage M1 and M2 subgroup marker genes and polarization-associated genes were analyzed by RT-qPCR. The number of M1 and M2 macrophages in our mouse model of OIR was analyzed by flow cytometry at different time points during the progression of OIR. Immunofluorescence whole mount staining of the retinas of mice with OIR was performed at different time points to examine the influx of macrophages, as well as the morphological characteristics and roles of M1 and M2 macrophages...
June 13, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28626028/excess-centrosomes-induce-p53-dependent-senescence-without-dna-damage-in-endothelial-cells
#3
Zhixian Yu, Dana L Ruter, Erich J Kushner, Victoria L Bautch
Tumor blood vessels support tumor growth and progression. Centrosomes are microtubule organization centers in cells, and often up to 30% of tumor endothelial cells (ECs) acquire excess (>2) centrosomes. Although excess centrosomes can lead to aneuploidy and chromosome instability in tumor cells, how untransformed ECs respond to excess centrosomes is poorly understood. We found that the frequency of primary human ECs with excess centrosomes was quickly reduced in a p53-dependent manner. Excess centrosomes in ECs were associated with p53 phosphorylation at Ser33, increased p21 levels, and decreased cell proliferation and expression of senescence markers, but independent of DNA damage and apoptosis...
June 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28618962/amelioration-of-dalton-s-lymphoma-induced-angiogenesis-by-melatonin
#4
Rani Kumari, Kavita Rawat, Anupma Kumari, Anju Shrivastava
For tumor to grow beyond 1-2 mm(3) size, tumor recruits new blood vessels referred as angiogenesis; therefore, targeting angiogenesis can be a promising strategy to suppress cancer progression. In this study, in order to develop a good angiogenesis model, we investigated effect of Dalton's lymphoma on angiogenesis and further monitored the role of melatonin on regulation of angiogenesis. To evaluate angiogenesis, endothelial cells were isolated from main thoracic aorta and cultured in vitro in the presence or absence of Dalton's lymphoma supplemented with or without melatonin to monitor their role on its proliferation and migration, a hallmark of angiogenesis...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28589930/dynamic-alterations-in-decoy-vegf-receptor-1-stability-regulate-angiogenesis
#5
Joshua M Boucher, Ryan P Clark, Diana C Chong, Kathryn M Citrin, Lyndsay A Wylie, Victoria L Bautch
Blood vessel expansion is driven by sprouting angiogenesis of endothelial cells, and is essential for development, wound healing and disease. Membrane-localized vascular endothelial growth factor receptor-1 (mVEGFR1) is an endothelial cell-intrinsic decoy receptor that negatively modulates blood vessel morphogenesis. Here we show that dynamic regulation of mVEGFR1 stability and turnover in blood vessels impacts angiogenesis. mVEGFR1 is highly stable and constitutively internalizes from the plasma membrane. Post-translational palmitoylation of mVEGFR1 is a binary stabilization switch, and ligand engagement leads to depalmitoylation and lysosomal degradation...
June 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28584077/vascular-sprouts-induce-local-attraction-of-proangiogenic-neutrophils
#6
Gustaf Christoffersson, Jalal Lomei, Paul O'Callaghan, Johan Kreuger, Stefan Engblom, Mia Phillipson
Angiogenesis, the growth of new blood vessels, is a complex process requiring the orchestration of numerous different cell types, growth factors, and chemokines. Some of the recently acknowledged actors in this process are immune cells. They accumulate at hypoxic sites, but the kinetics, dynamics, and regulation of that trafficking are unknown. In this study, we used intravital and live cell imaging to understand how neutrophils and macrophages migrate and behave at angiogenic sites. We developed two reproducible models of angiogenesis: one by transplanting isolated and hypoxic pancreatic islets into the cremaster muscles of mice, and another by in vitro coculturing of mouse aortic rings with neutrophils...
June 5, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28570946/microfabricated-blood-vessels-undergo-neoangiogenesis
#7
Kyle A DiVito, Michael A Daniele, Steven A Roberts, Frances S Ligler, André A Adams
The greatest ambition and promise of tissue engineering is to manufacture human organs. Before "made-to-measure" tissues can become a reality [1-3], however, three-dimensional tissues must be reconstructed and characterized. The current inability to manufacture operational vasculature has limited the growth of engineered tissues. Here, free-standing, small diameter blood vessels with organized cell layers that recapitulate normal biological functionality are fabricated using microfluidic technology. Over time in culture, the endothelial cells form a monolayer on the luminal wall and remodel the scaffold with human extracellular matrix proteins...
May 19, 2017: Biomaterials
https://www.readbyqxmd.com/read/28570535/visualization-and-quantitative-analysis-of-embryonic-angiogenesis-in-xenopus-tropicalis
#8
Jiyeon Ohk, Hosung Jung
Blood vessels supply oxygen and nutrients throughout the body, and the formation of the vascular network is under tight developmental control. The efficient in vivo visualization of blood vessels and the reliable quantification of their complexity are key to understanding the biology and disease of the vascular network. Here, we provide a detailed method to visualize blood vessels with a commercially available fluorescent dye, human plasma acetylated low density lipoprotein DiI complex (DiI-AcLDL), and to quantify their complexity in Xenopus tropicalis...
May 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28561127/integrating-perfusable-vascular-networks-with-a-three-dimensional-tissue-in-a-microfluidic-device
#9
Yuji Nashimoto, Tomoya Hayashi, Itsuki Kunita, Akiko Nakamasu, Yu-Suke Torisawa, Masamune Nakayama, Hisako Takigawa-Imamura, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa
Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen...
May 31, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28557377/wet-amd-on-a-chip-modeling-outer-blood-retinal-barrier-in-vitro
#10
Minhwan Chung, Somin Lee, Byung Joo Lee, Kyungmin Son, Noo Li Jeon, Jeong Hun Kim
Choroidal neovascularization (CNV) in the retinal pigment epithelium (RPE)-choroid complex constituting outer blood retinal barrier (oBRB) is a critical pathological step in various ophthalmic diseases, which results in blindness, such as wet type age-related macula degeneration. Current in vitro experimental models using petri dishes or transwell are unable to study CNV morphogenesis. Here, a unique organotypic eye-on-a-chip model is described that mimics the RPE-choroid complex in vitro. This model consists of an RPE monolayer and adjacent perfusable blood vessel network, which is supporting barrier function of oBRB...
May 29, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28551771/synergistic-effects-of-chuanxiong-chishao-herb-pair-on-promoting-angiogenesis-at-network-pharmacological-and-pharmacodynamic-levels
#11
Yan Wang, Gang Guo, Bin-Rui Yang, Qi-Qi Xin, Qi-Wen Liao, Simon Ming-Yuen Lee, Yuan-Jia Hu, Ke-Ji Chen, Wei-Hong Cong
OBJECTIVE: To investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting angiogenesis in silico and in vivo. METHODS: The mechanisms of action of an herb-pair, Chuanxiong- Chishao, were investigated using the network pharmacological and pharmacodynamic strategies involving computational drug target prediction and network analysis, and experimental validation. A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks, including prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao...
May 27, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28534996/ginsenoside-rd-regulates-the-akt-mtor-p70s6k-signaling-cascade-and-suppresses-angiogenesis-and-breast-tumor-growth
#12
Eryun Zhang, Hailian Shi, Li Yang, Xiaojun Wu, Zhengtao Wang
Blockade of angiogenesis is an important approach for cancer treatment and prevention. In the present study, we investigated the effect of ginsenoside Rd (Rd) on angiogenesis in vitro and in vivo. Our results demonstrated that Rd inhibited vascular endothelial growth factor (VEGF)-induced migration, tube formation and proliferation of primary cultured human umbilical vascular endothelial cells (HUVECs) dose‑dependently. Furthermore, Rd abrogated VEGF-induced sprouting of the vessels from aortic rings, and inhibited vascular formation in the Matrigel plug assay in vivo...
May 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28526753/altered-feto-placental-vascularization-feto-placental-malperfusion-and-fetal-growth-restriction-in-mice-with-egfl7-loss-of-function
#13
Lauretta A Lacko, Romulo Hurtado, Samantha Hinds, Michael G Poulos, Jason M Butler, Heidi Stuhlmann
EGFL7 is a secreted, angiogenic factor produced by embryonic endothelial cells. To understand its role in placental development, we established a novel Egfl7 knockout mouse. The mutant mice have gross defects in chorioallantoic branching morphogenesis and placental vascular patterning. Microangiography and 3D imaging revealed patchy perfusion of Egfl7(-/-) placentas marked by impeded blood conductance through sites of narrowed vessels. Consistent with poor feto-placental perfusion, Egfl7 knockout resulted in reduced placental weight and fetal growth restriction...
May 19, 2017: Development
https://www.readbyqxmd.com/read/28505675/-anti-lymph-angiogenic-strategies-to-improve-corneal-graft-survival
#14
Felix Bock, Claus Cursiefen
Corneal transplantation (keratoplasty) is the most frequently performed form of transplantation worldwide. A rejection reaction against the transplant is the main complication occurring after transplantation in an already vascularized, so-called "high-risk" recipient eye. Our group has shown that clinically invisible lymphatic vessels play a crucial role in the induction of a rejection reaction against the corneal graft, and that anti-(lymph)angiogenic therapies in the mouse model of keratoplasty can significantly improve transplant survival...
May 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28469004/sprouting-strategies-and-dead-ends-in-anti-angiogenic-targeting-of-nets
#15
Patricia Carrasco, Iratxe Zuazo-Gaztelu, Oriol Casanovas
Neuroendocrine tumors (NETs) are a heterogeneous group of neoplasms that arise from cells of the neuroendocrine system. NETs are characterized by being highly vascularized tumors that produce large amounts of proangiogenic factors. Due to their complexity and heterogeneity, progress in the development of successful therapeutic approaches has been limited. For instance, standard chemotherapy-based therapies have proven to be poorly selective for tumor cells and toxic for normal tissues. Considering the urge to develop an efficient therapy to treat NET patients, vascular targeting has been proposed as a new approach to block tumor growth...
May 3, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28466788/vanadium-iii-l-cysteine-enhances-the-sensitivity-of-murine-breast-adenocarcinoma-cells-to-cyclophosphamide-by-promoting-apoptosis-and-blocking-angiogenesis
#16
Abhishek Basu, Arin Bhattacharjee, Rathindranath Baral, Jaydip Biswas, Amalesh Samanta, Sudin Bhattacharya
Various epidemiological and preclinical studies have already established the cancer chemopreventive potential of vanadium-based compounds. In addition to its preventive efficacy, studies have also indicated the abilities of vanadium-based compounds to induce cell death selectively toward malignant cells. Therefore, the objective of the present investigation is to improve the therapeutic efficacy and toxicity profile of an alkylating agent, cyclophosphamide, by the concurrent use of an organovanadium complex, vanadium(III)-l-cysteine...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28459441/mural-lymphatic-endothelial-cells-regulate-meningeal-angiogenesis-in-the-zebrafish
#17
Neil I Bower, Katarzyna Koltowska, Cathy Pichol-Thievend, Isaac Virshup, Scott Paterson, Anne K Lagendijk, Weili Wang, Benjamin W Lindsey, Stephen J Bent, Sungmin Baek, Maria Rondon-Galeano, Daniel G Hurley, Naoki Mochizuki, Cas Simons, Mathias Francois, Christine A Wells, Jan Kaslin, Benjamin M Hogan
Mural cells of the vertebrate brain maintain vascular integrity and function, play roles in stroke and are involved in maintenance of neural stem cells. However, the origins, diversity and roles of mural cells remain to be fully understood. Using transgenic zebrafish, we identified a population of isolated mural lymphatic endothelial cells surrounding meningeal blood vessels. These meningeal mural lymphatic endothelial cells (muLECs) express lymphatic endothelial cell markers and form by sprouting from blood vessels...
June 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28456951/s1p-synergizes-with-wall-shear-stress-and-other-angiogenic-factors-to-induce-endothelial-cell-sprouting-responses
#18
Camille L Duran, Roland Kaunas, Kayla J Bayless
Angiogenesis is the process of new blood vessel growth from pre-existing structures. During sprout initiation, endothelial cells (ECs) are activated by pro-angiogenic factors to degrade the basement membrane, migrate into the surrounding matrix, and form structures that anastomose to connect neighboring vessels. Sphingosine 1-phosphate (S1P) is a biologically active lysosphingolipid that is secreted by platelets and promotes angiogenesis under normal and pathological conditions by acting on ECs. In addition to biochemical factors, the endothelium is continuously subjected to mechanical forces in the form of wall shear stress (WSS) from fluid forces...
April 30, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28450937/whole-extracts-of-radix-achyranthis-bidentatae-and-radix-cyathulae-promote-angiogenesis-in-human-umbilical-vein-endothelial-cells-in-vitro-and-in-zebrafish-in-vivo
#19
Xuelin Zhou, Wing-Sum Siu, Cheng Zhang, Cheuk-Lun Liu, Ling Cheng, Hin-Fai Kwok, Chak-Hei Fung, Jacqueline Chor-Wing Tam, Ching-Po Lau, Clara Bik-San Lau, Ping-Chung Leung, Leung-Kim Hung, Chun-Hay Ko
Although Radix Achyranthis Bidentatae (RAB) and Radix Cyathulae (RC) are from two different medicinal plants, they are both used as 'Niu-Xi', a widely used traditional Chinese medicine that is believed to stimulate menstruation and affect bone injury. Angiogenesis is actively involved in treating these illnesses. The aim of the present study was to investigate whether the whole extracts of RAB and RC possess pro-angiogenic effects. In order to examine this idea whole extracts of RAB and RC were extracted with boiling water followed by ethanol, respectively...
March 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28445154/role-of-delta-like-4-in-jagged1-induced-tumour-angiogenesis-and-tumour-growth
#20
Chern Ein Oon, Esther Bridges, Helen Sheldon, Richard C A Sainson, Adrian Jubb, Helen Turley, Russell Leek, Francesca Buffa, Adrian L Harris, Ji-Liang Li
Delta-like 4 (DLL4) and Jagged1 (JAG1) are two key Notch ligands implicated in tumour angiogenesis. They were shown to have opposite effects on mouse retinal and adult regenerative angiogenesis. In tumours, both ligands are upregulated but their relative effects and interactions in tumour biology, particularly in tumour response to therapeutic intervention are unclear. Here we demonstrate that DLL4 and JAG1 displayed equal potency in stimulating Notch target genes in HMEC-1 endothelial cells but had opposing effects on sprouting angiogenesis in vitro...
June 20, 2017: Oncotarget
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