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https://www.readbyqxmd.com/read/28102095/significance-of-the-ionized-calcium-measurement-to-assess-calcium-status-in-osteopenic-osteoporosis-postmenopausal-outpatients
#1
Letizia Guiducci, Silvia Maffei, Laura Sabatino, Luc Zyw, Debora Battaglia, Alessandro Vannucci, Pietro Di Cecco, Cristina Vassalle
INTRODUCTION: Evaluation of calcium status is important in the osteoporotic risk assessment. Although guidelines indicate total calcium (tCa) as first-line measurement, directly measured ionized calcium (m-iCa), considered as the gold standard, is more and more often required. Aim of this study is to evaluate the agreement between m-iCa, tCa and iCa calculated from a formula based on total calcium and albumin (c-iCa) in osteopenic/osteoporotic postmenopausal outpatients. METHODS: A total of 140 postmenopausal outpatients, 41 osteopenic (OPN) and 99 osteoporotic (OP) were enrolled...
January 19, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28101973/heparin-cross-linked-collagen-sponge-scaffolds-improve-functional-regeneration-of-rat-tracheal-epithelium
#2
Ryosuke Nakamura, Akiko Tani, Susumu Yoshie, Masakazu Ikeda, Ikuo Wada, Akihiro Hazama, Yukio Nomoto, Yasuhiro Tada, Tatsuo Nakamura, Koichi Omori
Tracheal epithelial cells maintain airway homeostasis by mediating mucociliary clearance. Following tracheal reconstruction, timely epithelial regeneration is required to prevent respiratory compromise and infectious diseases. To achieve rapid tracheal epithelial regeneration, a heparin cross-linked collagen sponge containing fibroblast growth factor-2 (FGF-2) was prepared as a graft for tracheal reconstruction. The heparin cross-linked sponge exhibited a high FGF-2 retaining capacity, and tracheal epithelial and mesenchymal cells cultured in this sponge containing FGF-2 showed high proliferative capacities...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28101870/fibrillar-collagens
#3
Jordi Bella, David J S Hulmes
Fibrillar collagens (types I, II, III, V, XI, XXIV and XXVII) constitute a sub-group within the collagen family (of which there are 28 types in humans) whose functions are to provide three-dimensional frameworks for tissues and organs. These networks confer mechanical strength as well as signalling and organizing functions through binding to cellular receptors and other components of the extracellular matrix (ECM). Here we describe the structure and assembly of fibrillar collagens, and their procollagen precursors, from the molecular to the tissue level...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28100492/dynamic-assembly-of-ultrasoft-colloidal-networks-enables-cell-invasion-within-restrictive-fibrillar-polymers
#4
Alison M Douglas, Alexandros A Fragkopoulos, Michelle K Gaines, L Andrew Lyon, Alberto Fernandez-Nieves, Thomas H Barker
In regenerative medicine, natural protein-based polymers offer enhanced endogenous bioactivity and potential for seamless integration with tissue, yet form weak hydrogels that lack the physical robustness required for surgical manipulation, making them difficult to apply in practice. The use of higher concentrations of protein, exogenous cross-linkers, and blending synthetic polymers has all been applied to form more mechanically robust networks. Each relies on generating a smaller network mesh size, which increases the elastic modulus and robustness, but critically inhibits cell spreading and migration, hampering tissue regeneration...
January 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28100304/iron-responsive-like-elements-in-the-parasite-entamoeba-histolytica
#5
Liliana Soto-Castro, Laura Yuliana Plata-Guzman, Elisa Elvira Figueroa-Angulo, Jaeson Calla-Choque, Magda Reyes-Lopez, Mireya DE LA Garza, Nidia Leon-Sicairos, Jose Antonio Garzon-Tiznado, Rossana Arroyo, Claudia Leon-Sicairos
In Entamoeba histolytica, iron modulates virulence and gene expression via unknown regulatory mechanisms. The existence of a posttranscriptional iron regulatory system parallel to the IRE/IRP (Iron-Responsive Element/Iron Regulatory Protein) system in the protozoan Trichomonas vaginalis has recently been reported. Due to their evolutionary closeness and the importance of iron for growth and virulence in these protozoa, we hypothesized the existence of an IRE/IRP-like mechanism in E. histolytica. To determine the presence of IRE-like elements in some mRNAs from this parasite, we performed in silico analyses of the 5'- and 3'-UTRs of mRNAs encoding virulence factors and cytoskeleton, ribosomal, and metabolism proteins...
January 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28099001/conformational-dynamics-in-penicillin-binding-protein-2a-of-methicillin-resistant-staphylococcus-aureus-allosteric-communication-network-and-enablement-of-catalysis
#6
Kiran V Mahasenan, Rafael Molina, Renee Bouley, Maria T Batuecas, Jed F Fisher, Juan A Hermoso, Mayland Chang, Shahriar Mobashery
The mechanism of the β-lactam antibacterials is the functionally irreversible acylation of the enzymes that catalyze the cross-linking steps in the biosynthesis of their peptidoglycan cell wall. The Gram-positive pathogen Staphylococcus aureus uses one primary resistance mechanism. An enzyme, called penicillin-binding protein 2a (PBP2a), is brought into this biosynthetic pathway to complete the cross-linking. PBP2a effectively discriminates against the β-lactam antibiotics as potential inhibitors, and in favor of the peptidoglycan substrate...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28098463/absolute-configuration-of-native-oligomeric-proanthocyanidins-with-dentin-biomodification-potency
#7
Joo-Won Nam, Rasika S Phansalkar, David C Lankin, James B McAlpine, Ariene A Leme-Kraus, Cristina M P Vidal, Li-She Gan, Ana Bedran-Russo, Shao-Nong Chen, Guido F Pauli
The structurally complex oligomeric proanthocyanidins (OPACs) are promising biomimetic agents, capable of strengthening the macromolecular backbone of teeth via intermolecular and intermicrofibrillar cross-linking. This study establishes analytical methods capable of determining the absolute configuration of the catechin-type monomeric units of underivatized OPACs. This preserves the capacity of their biological evaluation, aimed at understanding the inevitably stereospecific interactions between the OPACs and dentin collagen...
January 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28098445/circular-and-chainlike-copper-ii-lanthanide-iii-complexes-generated-by-assembly-reactions-of-racemic-and-chiral-copper-ii-cross-linking-ligand-complexes-with-ln-iii-no3-3%C3%A2-6h2o-ln-iii-gd-iii-tb-iii-dy-iii
#8
Takahiro Ueno, Takeshi Fujinami, Naohide Matsumoto, Masaki Furusawa, Ryo Irie, Nazzareno Re, Takuya Kanetomo, Takayuki Ishida, Yukinari Sunatsuki
The 1:1 assembly reaction of the racemic form of the cross-linking ligand complex Na[Cu(II)L(dpen(1R2R/1S2S))] with Ln(III)(NO3)3·6H2O gave the centrosymmetric circular (Cu(II)Ln(III))2 complex [Cu(II)L(dpen(1R2R/1S2S))Ln(III)(NO3)2]2 (1Ln: Ln = Gd, Tb, Dy), while the reaction of the enantiopure form Na[Cu(II)L(dpen(1R2R))] with Ln(III)(NO3)3·6H2O gave the chiral chainlike (Cu(II)Ln(III))1∞ complex [Cu(II)L(dpen(1R2R))Ln(III)(NO3)2(CH3CN)]1∞·CH3CN (2Ln: Ln = Gd, Tb, Dy), where {Cu(II)L(dpen(1R2R))}(-) is (N-((1R,2R)-2-(((E)-3-ethoxy-2-oxybenzylidene)amino)-1,2-diphenylethyl)-2-oxybenzamide)copper(II) and {Cu(II)L(dpen(1R2R/1S2S))}(-) is the racemic mixture of {Cu(II)L(dpen(1R2R))}(-) and {Cu(II)L(dpen(1S2S))}(-)...
January 18, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28097869/a-solvent-free-processable-and-photo-patternable-hybrid-gate-dielectric-for-flexible-top-gate-organic-field-effect-transistors
#9
Jun Seon Kwon, Han-Wool Park, Do Hwan Kim, Young-Je Kwark
We report a novel solvent-free and direct photo-patternable poly[(mercaptopropyl)methyl-siloxane] (PMMS) hybrid dielectric for flexible top-gate organic field-effect transistors (OFETs), utilizing a photo-activated thiol-ene reaction under UV irradiation of 254 nm to induce cross-linking, even in air and at low temperature. In particular, a solvent-free PMMS-f dielectric film, for which an optimal cross-linking density is shown by a well-organized molar ratio between thiol and vinyl in the thiol-ene reaction, exhibited a high dielectric constant (5...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28097682/evaluation-of-perforated-demineralized-dentin-scaffold-on-bone-regeneration-in-critical-size-sheep-iliac-defects
#10
Md Arafat Kabir, Masaru Murata, Toshiyuki Akazawa, Kaoru Kusano, Katsuhisa Yamada, Manabu Ito
OBJECTIVES: Regenerating critical-size bone injury is a major problem that continues to inspire the design of new graft materials. Therefore, tissue engineering has become a novel approach for targeting bone regeneration applications. Human teeth are a rich source of stem cells, matrix, trace metal ions, and growth factors. A vital tooth-derived demineralized dentin matrix is acid-insoluble and composed of cross-linked collagen with growth factors. In this study, we recycled human non-functional tooth into a unique geometric dentin scaffold, entitled perforated root-demineralized dentin matrix (PR-DDM)...
January 17, 2017: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/28096358/repair-shielding-of-platinum-dna-lesions-in-testicular-germ-cell-tumors-by-high-mobility-group-box-protein-4-imparts-cisplatin-hypersensitivity
#11
Samuel G Awuah, Imogen A Riddell, Stephen J Lippard
Cisplatin is the most commonly used anticancer drug for the treatment of testicular germ cell tumors (TGCTs). The hypersensitivity of TGCTs to cisplatin is a subject of widespread interest. Here, we show that high-mobility group box protein 4 (HMGB4), a protein preferentially expressed in testes, uniquely blocks excision repair of cisplatin-DNA adducts, 1,2-intrastrand cross-links, to potentiate the sensitivity of TGCTs to cisplatin therapy. We used CRISPR/Cas9-mediated gene editing to knockout the HMGB4 gene in a testicular human embryonic carcinoma and examined cellular responses...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28094986/improving-internal-cell-colonization-of-porous-scaffolds-with-chemical-gradients-produced-by-plasma-assisted-approaches
#12
Eloisa Sardella, Rania A Salama, Gihan H Waly, A Nour Habib, Pietro Favia, Roberto Gristina
Cell colonization of the surrounding environment is a very significant process in both physiological and pathological events. In order to understand the tissue regeneration process and thereby provide guidance principles for designing new biomaterials, it is of paramount importance to study the cell colonization in the presence of physical, chemical and biological cues. Flat "gradient" materials are generally used with this purpose. Three dimensional (3D) gradient scaffolds mimicking more precisely the situation in vivo, are somewhat more complex to fabricate and characterize...
January 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28094979/photo-induced-cross-linking-of-short-furan-modified-dna-on-surfaces
#13
Cinthya Yamila Véliz Montes, Henry Memczak, Ellen Gyssels, Tomas Torres, Annemieke Madder, Rudolf J Schneider
We report for the first time the formation of site-specifically interstrand cross-linked (ICL) surface-immobilized furan-modified DNA duplexes via singlet oxygen. 1O2, necessary for effecting furan-mediated ICL formation, was produced in situ using methylene blue or a zinc phthalocyanine derivative (TT1) as photosensitizers. Via surface plasmon resonance spectroscopy we show that surface ICL was achieved and established a robust link which enhances the stability of the 12-mer duplex even after surface regeneration...
January 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28094390/simultaneous-measurement-of-the-young-s-modulus-and-the-poisson-ratio-of-thin-elastic-layers
#14
Wolfgang Gross, Holger Kress
The behavior of cells and tissue is greatly influenced by the mechanical properties of their environment. For studies on the interactions between cells and soft matrices, especially those applying traction force microscopy the characterization of the mechanical properties of thin substrate layers is essential. Various techniques to measure the elastic modulus are available. Methods to accurately measure the Poisson ratio of such substrates are rare and often imply either a combination of multiple techniques or additional equipment which is not needed for the actual biological studies...
January 17, 2017: Soft Matter
https://www.readbyqxmd.com/read/28094370/probing-sub-nano-level-molecular-packing-and-correlated-positron-annihilation-characteristics-of-ionic-cross-linked-chitosan-membranes-using-positron-annihilation-spectroscopy
#15
Rui Xia, Xingzhong Cao, Meizhen Gao, Peng Zhang, Minfeng Zeng, Baoyi Wang, Long Wei
Chitosan, CS, cross-linked with bivalent palladium has shown enhanced mechanical and thermal properties depending on the transformation of the structure at a microscopic scale. In the present study, CS directly cross-linked by palladium cation membranes (CS-cr-PM) was prepared through a solution-casting method. Mobility of chitosan chains were greatly reduced after crosslinking, making a great reduction in the swelling ratio studied by a water-swelling degree measurement, which led to an improvement in molecular chain rigidity...
January 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28094355/biopolymer-chitosan-based-supramolecular-hydrogels-as-solid-state-electrolytes-for-electrochemical-energy-storage
#16
Lujie Cao, Mingyang Yang, Dong Wu, Fucong Lyu, Zhifang Sun, Xiongwei Zhong, Hui Pan, Hongtao Liu, Zhouguang Lu
A biopolymer-chitosan based Supramolecular Hydrogel type solid state Electrolyte (SHE) is prepared via a simple and fast cross-linking between chitosan and Li(+)/Ag(+). The obtained SHE itself shows high thermal stability and excellent flexible and mouldable properties. When integrated in an asymmetric supercapacitor with MnO2 as the positive electrode and active carbon as the negative electrode, it can survive more than 10 000 cycles with the areal capacity of 10 mF cm(-2) at the 1.8 mA cm(-2) current density...
January 17, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28093528/a-b-cell-epitope-peptide-derived-from-the-major-grass-pollen-allergen-phl-p-1-boosts-allergen-specific-secondary-antibody-responses-without-allergen-specific-t-cell-help
#17
Meena Narayanan, Raphaela Freidl, Margarete Focke-Tejkl, Ulrike Baranyi, Thomas Wekerle, Rudolf Valenta, Birgit Linhart
More than 40% of allergic patients suffer from grass pollen allergy. Phl p 1, the major timothy grass pollen allergen, belongs to the cross-reactive group 1 grass pollen allergens that are thought to initiate allergic sensitization to grass pollen. Repeated allergen encounter boosts allergen-specific IgE production and enhances clinical sensitivity in patients. To investigate immunological mechanisms underlying the boosting of allergen-specific secondary IgE Ab responses and the allergen epitopes involved, a murine model for Phl p 1 was established...
January 16, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28093366/strategy-for-constructing-vascularized-adipose-units-in-poly-l-glutamic-acid-hydrogel-porous-scaffold-through-inducing-in-situ-formation-of-ascs-spheroids
#18
Kunxi Zhang, Li Song, Jia Wang, Shifeng Yan, Guifei Li, Lei Cui, Jingbo Yin
: Vascularization is of great importance to adipose tissue regeneration. Here we introduced a paradigm that using scaffold to induce ASC spheroids, so to promote vascularized adipose tissue regeneration. Poly (L-glutamic acid) (PLGA) was activated by EDC, followed by being cross-linked by Adipic dihydrazide (ADH) to form a homogeneous hydrogel. Lyophilization was then carried out to create porous structure. The PLGA hydrogel scaffold possessed a significant swollen hydrophilic network to weaken cell-scaffold adhesion but drive ASCs to aggregate to form spheroids...
January 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28093333/characterization-of-oral-disintegrating-film-of-peanut-skin-extract-potential-route-for-buccal-delivery-of-phenolic-compounds
#19
Marcela Tedesco, Carla Monaco, Rosemary A Carvalho
This paper aimed to develop and characterize oral disintegrating films (ODF) based on gelatin and hydroxypropyl methylcellulose (HPMC) incorporated with peanut skin extract (PSE) as phenolic compounds vehicle. Films were prepared by casting technique varying the polymer ratio (GEL:HPMC 100:0, 25:75, 50:50, 75:25, 0:100) and PSE concentration (20 and 30g/100g film-forming solution). Formulations with high content of gelatin presented insoluble complex possibly due to cross-linking between gelatin and polyphenols...
January 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28093072/in-vitro-effects-of-hyaluronic-acid-on-human-periodontal-ligament-cells
#20
Masako Fujioka-Kobayashi, Heinz-Dieter Müller, Andrea Mueller, Adrian Lussi, Anton Sculean, Patrick R Schmidlin, Richard J Miron
BACKGROUND: Hyaluronic acid (HA) has been reported to have a positive effect on periodontal wound healing following nonsurgical and surgical therapy. However, to date, a few basic in vitro studies have been reported to investigating the potential of HA on human periodontal ligament (PDL) cell regeneration. Therefore, the aim of this study was to investigate the effect of HA on PDL cell compatibility, proliferation, and differentiation in vitro. METHODS: Either non-cross-linked (HA_ncl) or cross-linked (HA_cl) HA was investigated...
January 16, 2017: BMC Oral Health
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