keyword
MENU ▼
Read by QxMD icon Read
search

Cross-linking

keyword
https://www.readbyqxmd.com/read/29346675/in-search-of-the-best-xenogeneic-material-for-a-paediatric-conduit-an-experimental-study
#1
Irina Y Zhuravleva, Nataliya R Nichay, Yuriy Y Kulyabin, Tatyana P Timchenko, Alexander A Korobeinikov, Yuliya F Polienko, Svetlana S Shatskaya, Elena V Kuznetsova, Alexey V Voitov, Alexander V Bogachev-Prokophiev, Alexander M Karaskov
OBJECTIVES: The development of calcification-resistant bioprosthetic materials is a very important challenge for paediatric surgery. The subcutaneous implantation in rats is the well-known first-stage model for this kind of research. Using this model, we aimed to compare calcification of the porcine aortic wall and bovine pericardium and jugular vein wall cross-linked with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (DE). We also determined the efficacy of DE-preserved tissue modification with 2-(2-carboxyethylamino)ethylidene-1,1-bisphosphonic acid (CEABA)...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29346493/antimicrobial-studies-using-the-therapeutic-tissue-cross-linking-agent-sodium-hydroxymethylglycinate-implication-for-treating-infectious-keratitis
#2
Patrick B Rapuano, Alexandra H Scanameo, Daeryl E Amponin, Sefy A Paulose, Mariya Zyablitskaya, Anna Takaoka, Leejee H Suh, Takayuki Nagasaki, Stephen L Trokel, David C Paik
Purpose: Our recent studies raise the possibility of using sodium hydroxymethylglycinate (SMG), for pharmacologic therapeutic tissue cross-linking (TXL) of the cornea. The present study was performed to evaluate the antimicrobial effects of SMG for potential use in treating infectious keratitis. Methods: In initial (group 1) experiments, methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa (PA) were treated with SMG (10-40 mM) for 10 to 120 minutes...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29345705/contact-mechanics-for-polydimethylsiloxane-from-liquid-to-solid
#3
L Dorogin, B N J Persson
Adhesion between a glass ball and a polydimethylsiloxane (PDMS) sample is dependent on the PDMS cross-link density, and the transformation of the material from the uncrosslinked liquid state to the fully crosslinked solid state is investigated in this study. The physical picture reflected a gradual transition from capillary forces driven contact mechanics to the classical Johnson-Kendall-Roberts (JKR)-type contact mechanics. PDMS was produced by mixing the base fluid and a cross-linker at a ratio of 10 : 1 and allowed to slowly cross-link at room temperature with simultaneous measurement of the ball-PDMS interaction force...
January 18, 2018: Soft Matter
https://www.readbyqxmd.com/read/29345453/poly-glycidol-coating-on-ultrahigh-molecular-weight-polyethylene-for-reduced-biofilm-growth
#4
Jacob N Lockhart, Thomas J Spoonmore, Michael W McCurdy, Bridget R Rogers, Scott A Guelcher, Eva Harth
Semibranched poly(glycidol) (PG-OH) and poly(glycidol allylglycidyl ether) (PG-Allyl) coatings were formed on ultrahigh molecular weight polyethylene (UMWPE) in a unique two-step process which included radiation of UHMWPE followed by grafting of PG-OH or PG-Allyl to the surface via free radical cross-linking. Resulting surfaces were extensively characterized by FTIR-ATR, XPS, fluorescent microscopy, and contact goniometry. The performance was evaluated using the most prominent biofilm-forming bacteria Staphylococcus aureus for 24 and 48 h...
January 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29345346/galectin-3-type-c-self-association-on-neutrophil-surfaces-the-carbohydrate-recognition-domain-regulates-cell-function
#5
Martina Sundqvist, Amanda Welin, Jonas Elmwall, Veronica Osla, Ulf J Nilsson, Hakon Leffler, Johan Bylund, Anna Karlsson
Galectin-3 is an endogenous β-galactoside-binding lectin comprising a carbohydrate recognition domain (CRD) linked to a collagen-like N-domain. Both domains are required for galectin-3 to induce cellular effects; a C-terminal fragment of galectin-3, galectin-3C, containing the CRD but lacking the N-domain, binds cell surface glycoconjugates but does not induce cellular effects since cross-linking promoted by the N-domain is thought to be required. Instead, galectin-3C is proposed to antagonize the effects of galectin-3 by competing for binding sites...
January 10, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29343618/retention-of-acholeplasma-laidlawii-by-sterile-filtration-membranes-effect-of-cultivation-medium-and-filtration-temperature
#6
Alexander Helling, Hannes König, Felix Seiler, Ralph Berkholz, Volkmar Thom, Milan Polakovic
This experimental study compares cell size, zeta potential and the ability to penetrate tailor-made size exclusion membrane filters of mycoplasma A. laidlawii cultivated in five different cultivation media. The influence of relevant filtration process parameters, in particular transmembrane pressure and filtration temperature, on their respective retention was tested. The impact of the filtration temperature was further evaluated for the Gram-negative bacteria species Brevundimonas diminuta, the Gram-positive bacteria species Staphylococcus epidermidis, the Pseudomonas phage PP7 and the mycoplasma species M...
January 17, 2018: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29343546/p62-filaments-capture-and-present-ubiquitinated-cargos-for-autophagy
#7
Gabriele Zaffagnini, Adriana Savova, Alberto Danieli, Julia Romanov, Shirley Tremel, Michael Ebner, Thomas Peterbauer, Martin Sztacho, Riccardo Trapannone, Abul K Tarafder, Carsten Sachse, Sascha Martens
The removal of misfolded, ubiquitinated proteins is an essential part of the protein quality control. The ubiquitin-proteasome system (UPS) and autophagy are two interconnected pathways that mediate the degradation of such proteins. During autophagy, ubiquitinated proteins are clustered in a p62-dependent manner and are subsequently engulfed by autophagosomes. However, the nature of the protein substrates targeted for autophagy is unclear. Here, we developed a reconstituted system using purified components and show that p62 and ubiquitinated proteins spontaneously coalesce into larger clusters...
January 17, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29342928/protein-oxidation-and-sensory-quality-of-brine-injected-pork-loins-added-ascorbate-or-extracts-of-green-tea-or-mat%C3%A3-during-chill-storage-in-high-oxygen-modified-atmosphere
#8
Sisse Jongberg, Mari Ann Tørngren, Leif H Skibsted
Background: Ascorbate is often applied to enhance stability and robustness of brine-injected pork chops sold for retail, but may affect protein oxidation, while plant extracts are potential substitutes. Methods: Brine-injected pork chops (weight-gain ~12%, NaCl ~0.9%) prepared with ascorbate (225 ppm), green tea extract (25 ppm gallic acid equivalents (GAE)), or maté extract (25 ppm GAE) stored (5 °C, seven days) in high-oxygen atmosphere packaging (MAP: 80% O₂ and 20% CO₂) were analyzed for color changes, sensory quality, and protein oxidation compared to a control without antioxidant...
January 15, 2018: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29342061/the-effect-of-alternative-bearing-surfaces-on-the-risk-of-revision-due-to-infection-in-minimally-stabilized-total-knee-replacement-an-analysis-of-326-603-prostheses-from-the-australian-orthopaedic-association-national-joint-replacement-registry
#9
Christopher J Vertullo, Peter L Lewis, Yi Peng, Stephen E Graves, Richard N de Steiger
BACKGROUND: The effect of alternative bearing materials on the risk of revision due to infection after total knee replacement remains uncertain. By reducing the immunomodulating polyethylene wear-particle burden and with different substrate bacterial adhesion properties, Oxinium oxidized zirconium and cross-linked polyethylene (XLPE) could alter infection risk. The purpose of the current study was to analyze the risk of revision for infection in 3 comparisons of bearing combinations. METHODS: To evaluate the risk of revision for infection with XLPE, cobalt-chromium (CoCr) on XLPE was compared with CoCr on non-cross-linked polyethylene (NXLPE)...
January 17, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29342060/creep-and-wear-in-vitamin-e-infused-highly-cross-linked-polyethylene-cups-for-total-hip-arthroplasty-a-prospective-randomized-controlled-trial
#10
G Rochcongar, G Buia, E Bourroux, J Dunet, V Chapus, C Hulet
BACKGROUND: Aseptic loosening, the most common indication for revision surgery in total hip arthroplasty, can result from osteolysis caused by polyethylene (PE) wear particles. PE wear is increased by age-related oxidation of PE and free radicals emerging during irradiation cross-linking. Diffusion of vitamin E into PE stabilizes free radicals to maintain the biomechanical properties of PE. The purpose of this study was to determine whether vitamin E-infused highly cross-linked PE cups could reduce wear rates...
January 17, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29341838/immobilization-of-cross-linked-tannase-enzyme-on-multi-walled-carbon-nanotubes-and-its-catalytic-behavior
#11
Chong-Boon Ong, Mohamad S M Annuar
Immobilization of cross-linked tannase on pristine multi-walled carbon nanotubes (MWCNT) was successfully carried out. Cross-linking of tannase molecules was made via glutaraldehyde. The immobilized tannase exhibited significantly improved pH, thermal, and recycling stabilities. The optimal pH for both free- and immobilized tannase were observed at pH 5.0 with optimal operating temperature at 30 °C. Moreover, immobilized enzyme retained greater biocatalytic activities upon ten-repeated uses compared to free enzyme in solution...
January 17, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29341589/metal-organic-frameworks-mofs-derived-hierarchical-co3o4-structures-as-efficient-sensing-materials-for-acetone-detection
#12
Rui Zhang, Tingting Zhou, Lili Wang, Tong Zhang
High sensitive and stable gas sensors have attracted much attention because they are the key to innovations in the fields of environment, health, energy savings and security, etc. Sensing materials, which influence the practical sensing performance, are the crucial parts for gas sensors. Metal-organic frameworks (MOFs) are considered as alluring sensing materials for gas sensors due to the possession of high specific surface area, unique morphology, abundant metal sites and functional linkers. Herein, four kinds of porous hierarchical Co3O4 structures have been selectively controlled by optimizing the thermal decomposition (temperature, rate and atmosphere) using ZIF-67 as precursor that was obtained from coprecipitation method with the co-assistant of cobalt salt and 2-methylimidazole in the solution of methanol...
January 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29341305/plasmid-templated-control-of-dna-cyclodextrin-nanoparticle-morphology-through-molecular-vector-design-for-effective-gene-delivery
#13
Laura Gallego-Yerga, Juan M Benito, Laura Blanco-Fernández, María Martínez-Negro, Itziar Vélaz, Emilio Aicart, Elena Junquera, Carmen Ortiz Mellet, Conchita Tros de Ilarduya, Jose M Garcia Fernandez
Engineering self-assembled superstructures through complexation of plasmid DNA (pDNA) and single-isomer nanometric size macromolecules (molecular nanoparticles) is a promising strategy for gene delivery. Notably, the functionality and overall architecture of the vector can be precisely moulded at the atomic level by chemical tailoring, enabling unprecedented opportunities for structure/self-assembling/pDNA delivery relationship studies. Beyond this notion, by judiciously preorganizing the functional elements in cyclodextrin (CD)-based molecular nanoparticles through covalent dimerization, here we demonstrate that the morphology of the resulting nanocomplexes (CDplexes) can be tuned, from spherical to ellipsoidal, rod-type or worm-like nanoparticles, which makes it possible to gain understanding of their shape-dependent transfection properties...
January 17, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29341267/cross-linking-of-thiolated-paclitaxel-oligo-p-phenylene-vinylene-conjugates-aggregates-inside-tumor-cells-leads-to-chemical-locks-that-increase-drug-efficacy
#14
Lingyun Zhou, Fengting Lv, Libing Liu, Guizhi Shen, Xuehai Yan, Guillermo C Bazan, Shu Wang
How to reduce the resistance of certain tumor cells to paclitaxel (PTX) and related taxoid anticancer drugs is a major challenge for improving cure rates. An oligo(p-phenylenevinylene) unit with thiol groups and a PTX unit (OPV-S-PTX), which enhances drug efficacy and reverses resistance is thus designed. The mechanism involves diffusion of OPV-S-PTX into the cell, where π-π interactions lead to aggregation. Cross-linking of the aggregates via oxidation of thiol groups is favored in tumor cells because of the higher reactive oxygen species (ROS) concentration...
January 17, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29341255/data-assisted-protein-structure-modeling-by-global-optimization-in-casp12
#15
Keehyoung Joo, Seungryong Heo, InSuk Joung, Seung Hwan Hong, Sung Jong Lee, Jooyoung Lee
In CASP12, two types of data-assisted protein structure modeling were experimented. Either SAXS experimental data or cross-linking experimental data was provided for a selected number of CASP12 targets, so that the CASP12 predictor can utilize the given data for better protein structure modeling. We devised two separate energy terms for SAXS data and cross-linking data to drive model structures into more native-like structures that satisfy the given experimental data as much as possible. In CASP11, we successfully performed protein structure modeling using simulated sparse and ambiguously-assigned NOE data and/or correct residue-residue contact information, where the only energy term that folded the protein into its native structure was the term which was originated from the given experimental data...
January 17, 2018: Proteins
https://www.readbyqxmd.com/read/29340411/multiplexed-detection-of-nucleic-acids-using-19f-nmr-chemical-shift-changes-based-on-dna-photo-cross-linking-of-3-vinylcarbazole-derivatives
#16
Shigetaka Nakamura, Yasuharu Takashima, Kenzo Fujimoto
The detection methodology for nucleic acids is a useful tool for the analysis of biological systems and diagnosis of diseases. We demonstrated the feasibility of the detection of any nucleic acids based on large chemical shifts via ultrafast DNA photo-cross-linking and the effects of substitution by 3-vinylcarbazole derivatives. These chemical shifts enable the sequence-specific detection of any strand using hybridization chain reaction.
January 17, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29339574/phase-diagram-of-carbon-and-the-factors-limiting-the-quantity-and-size-of-natural-diamonds
#17
Vladimir Blank, Valentin Churkin, Boris Kulnitskiy, Igor A Perezhogin, Alexey Kirichenko, Viktor Denisov, Sergey Erohin, Pavel Sorokin, Mikhail Popov
Phase diagrams of carbon, and those focusing on the graphite-to-diamond transitional conditions in particular, are of great interest for fundamental and applied research. The present study introduces a number of experiments carried out to convert graphite under high-pressure conditions, showing a formation of stable phase of fullerene-type onions cross-linked by sp3-bonds in the 55-115 GPa pressure range instead of diamonds formation (even at temperature 2000-3000 K) and the already formed diamonds turn into carbon onions...
January 17, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29339572/three-dimensional-cross-linking-composite-of-graphene-carbon-nanotube-and-si-nanoparticles-for-lithium-ion-batteries-anode
#18
Suyun Tian, Guannan Zhu, Yanping Tang, Xiaohua Xie, Qian Wang, Yufei Ma, Gu Qiao Ding, Xiaoming Xie
Various graphene-based Si nanocomposites have been reported to improve the performance of active materials in Li ion battery. However, these candidates still yield severe capacity fading due to the electrical disconnection and fracture caused by huge volume changes with extended cycles.Therefore, we design a novel three-dimensional cross-linking structure of graphene and single-wall carbon nanotube to encapsulate Si nanoparticles. The synthesized three-dimensional structure is ascribed to the excellent self-assembly of carbon nanotube with graphene oxide and a thermal treatment process at 900 ºC...
January 17, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29339550/relative-target-affinities-of-t-cell-dependent-bispecific-antibodies-determine-biodistribution-in-a-solid-tumor-mouse-model
#19
Danielle Mandikian, Nene Takahashi, Amy A Lo, Ji Li, Jeffrey Eastham-Anderson, Dionysos Slaga, Jason Ho, Maria Hristopoulos, Robyn Clark, Klara Totpal, Kedan Lin, Sean B Joseph, Mark S Dennis, Saileta Prabhu, Teemu T Junttila, C Andrew Boswell
Anti-HER2/CD3, a T cell-dependent bispecific antibody (TDB) construct, induces T cell-mediated cell death in cancer cells expressing HER2 by cross-linking tumor HER2 with CD3 on cytotoxic T cells, thereby creating a functional cytolytic synapse. TDB design is a very challenging process that requires consideration of multiple parameters. While therapeutic antibody design strategy is commonly driven by striving for the highest attainable antigen binding affinity, little is known about how the affinity of each TDB arm can affect the targeting ability of the other arm and the consequent distribution and efficacy...
January 16, 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29339518/iterative-optimization-yields-mcl-1-targeting-stapled-peptides-with-selective-cytotoxicity-to-mcl-1-dependent-cancer-cells
#20
Raheleh Rezaei Araghi, Gregory H Bird, Jeremy A Ryan, Justin M Jenson, Marina Godes, Jonathan R Pritz, Robert A Grant, Anthony Letai, Loren D Walensky, Amy E Keating
Bcl-2 family proteins regulate apoptosis, and aberrant interactions of overexpressed antiapoptotic family members such as Mcl-1 promote cell transformation, cancer survival, and resistance to chemotherapy. Discovering potent and selective Mcl-1 inhibitors that can relieve apoptotic blockades is thus a high priority for cancer research. An attractive strategy for disabling Mcl-1 involves using designer peptides to competitively engage its binding groove, mimicking the structural mechanism of action of native sensitizer BH3-only proteins...
January 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
keyword
keyword
43087
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"