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https://www.readbyqxmd.com/read/28339299/the-10th-gcc-closed-forum-rejected-data-gcp-in-bioanalysis-extract-stability-bav-processed-batch-acceptance-matrix-stability-critical-reagents-eln-and-data-integrity-and-counteracting-fraud
#1
Stephanie Cape, Rafiq Islam, Corey Nehls, John Allinson, Afshin Safavi, Patrick Bennett, James Hulse, Chris Beaver, Masood Khan, Shane Karnik, Maria Cruz Caturla, Steve Lowes, Adriana Iordachescu, Luigi Silvestro, Rabab Tayyem, Ron Shoup, Stephanie Mowery, Anahita Keyhani, Andrea Wakefield, Yinghe Li, Jennifer Zimmer, Javier Torres, Philippe Couerbe, Ardeshir Khadang, James Bourdage, Nicola Hughes, Kayode Awaiye, Brent Matthews, Saadya Fatmi, Rhonda Johnson, Christina Satterwhite, Mathilde Yu, Jenny Lin, Laura Cojocaru, Michele Fiscella, Eric Thomas, Kai Kurylak, John Kamerud, Zhongping John Lin, Wei Garofolo, Natasha Savoie, Mike Buonarati, Nadine Boudreau, Clark Williard, Yansheng Liu, Dominic Warrino, Prashant Kale, Neil Adcock, Radha Shekar, Edward O'Connor, Hanna Ritzen, Christina Sanchez, Roger Hayes, Mohammed Bouhajib, Simona Rizea Savu, Bruce Stouffer, Edward Tabler, Jing Tu, Chad Briscoe, Barry van der Strate, Paul Rhyne, Phyllis Conliffe, Ira DuBey, Jim Yamashita, Daniel Tang, Elizabeth Groeber, Jenifer Vija, Michele Malone, Mohamed Osman
The 10th Global CRO Council (GCC) Closed Forum was held in Orlando, FL, USA on 18 April 2016. In attendance were decision makers from international CRO member companies offering bioanalytical services. The objective of this meeting was for GCC members to meet and discuss scientific and regulatory issues specific to bioanalysis. The issues discussed at this closed forum included reporting data from failed method validation runs, GCP for clinical sample bioanalysis, extracted sample stability, biomarker assay validation, processed batch acceptance criteria, electronic laboratory notebooks and data integrity, Health Canada's Notice regarding replicates in matrix stability evaluations, critical reagents and regulatory approaches to counteract fraud...
March 24, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28330253/parameters-influencing-agrobacterium-mediated-transformation-system-in-safflower-genotypes-aks-207-and-pkv-pink
#2
Dipti Raghunath Dhumale, Prashant Raghunath Shingote, Mahendra Shankarrao Dudhare, Pravin Vishwanath Jadhav, Prashant Bhaskar Kale
Shoot regeneration in safflower (Carthamus tinctorius 'AKS 207' and 'PKV Pink') genetically transformed using Agrobacterium was used for assessing various constraints to the efficiency of transformation including infection period, virulence induction medium, co-cultivation period, bacterial titre, selection regime, and the natural phenolic compound acetosyringone. Transformation frequency was promising with 8-10-day-old cotyledonary leaf explants. Therefore, explants of that age cultured on Agrobacterium minimal medium (AB) containing 100 µM acetosyringone were infected with Agrobacterium (cell titre 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28326095/potential-of-legume-brassica-intercrops-for-forage-production-and-green-manure-encouragements-from-a-temperate-southeast-european-environment
#3
REVIEW
Ana M Jeromela, Aleksandar M Mikić, Svetlana Vujić, Branko Ćupina, Đorđe Krstić, Aleksandra Dimitrijević, Sanja Vasiljević, Vojislav Mihailović, Sandra Cvejić, Dragana Miladinović
Legumes and brassicas have much in common: importance in agricultural history, rich biodiversity, numerous forms of use, high adaptability to diverse farming designs, and various non-food applications. Rare available resources demonstrate intercropping legumes and brassicas as beneficial to both, especially for the latter, profiting from better nitrogen nutrition. Our team aimed at designing a scheme of the intercrops of autumn- and spring-sown annual legumes with brassicas for ruminant feeding and green manure, and has carried out a set of field trials in a temperate Southeast European environment and during the past decade, aimed at assessing their potential for yields of forage dry matter and aboveground biomass nitrogen and their economic reliability via land equivalent ratio...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28326001/the-role-of-plant-processing-for-the-cancer-preventive-potential-of-ethiopian-kale-brassica-carinata
#4
Grace Akinyi Odongo, Nina Schlotz, Corinna Herz, Franziska S Hanschen, Susanne Baldermann, Susanne Neugart, Bernhard Trierweiler, Lara Frommherz, Charles M A P Franz, Benard Ngwene, Abraham Wahid Luvonga, Monika Schreiner, Sascha Rohn, Evelyn Lamy
Background: Ethiopian kale (Brassica carinata) is a horticulturally important crop used as leafy vegetable in large parts of East and Southern Africa. The leaves are reported to contain high concentrations of health-promoting secondary plant metabolites. However, scientific knowledge on their health benefits is scarce. Objective: This study aimed to determine the cancer preventive potential of B. carinata using a human liver in vitro model focusing on processing effects on the pattern of secondary plant metabolites and bioactivity...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28324953/electrical-resistance-increases-at-the-tissue-electrode-interface-as-an-early-response-to-nucleus-accumbens-deep-brain-stimulation
#5
Rajas P Kale, Abbas Z Kouzani, Julian Berk, Ken Walder, Michael Berk, Susannah J Tye
The therapeutic actions of deep brain stimulation are not fully understood. The early inflammatory response of electrode implantation is associated with symptom relief without electrical stimulation, but is negated by anti-inflammatory drugs. Early excitotoxic necrosis and subsequent glial scarring modulate the conductivity of the tissue-electrode interface, which can provide some detail into the inflammatory response of individual patients. The feasibility of this was demonstrated by measuring resistance values across a bipolar electrode which was unilaterally implanted into the nucleus accumbens of a rat while receiving continuous deep brain stimulation with a portable back-mounted device using clinical parameters (130Hz, 200μA, 90μs) for 3 days...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28300944/phytophagy-of-the-predator-podisus-nigrispinus-dallas-1851-hemiptera-pentatomidae-fed-on-prey-and-brassicaceae
#6
J F J Grigolli, M M Kubota Grigolli, D G Ramalho, A L Martins, A M Vacari, S A De Bortoli
The purpose of this study was to investigate the development and reproduction of the zoophytophagous predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) fed kale, broccoli and cabbage affects its. Nymphs and adults of this predator were fed on larvae of Plutella xylostella (L.) (Lepidoptera: Plutellidae) as prey with kale, cabbage, or broccoli. In the nymph period, the duration and prey consumption were similar with all the Brassicacea cultivar. However, nymph viability was higher for predators with broccoli leaves...
March 13, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28288583/genetics-and-fine-mapping-of-a-purple-leaf-gene-bopr-in-ornamental-kale-brassica-oleracea-l-var-acephala
#7
Xiao-Ping Liu, Bao-Zhen Gao, Feng-Qing Han, Zhi-Yuan Fang, Li-Mei Yang, Mu Zhuang, Hong-Hao Lv, Yu-Mei Liu, Zhan-Sheng Li, Cheng-Cheng Cai, Hai-Long Yu, Zhi-Yuan Li, Yang-Yong Zhang
BACKGROUND: Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular ornamental plant. In this study, we report the fine mapping and analysis of a candidate purple leaf gene using a backcross population and an F2 population derived from two parental lines: W1827 (with white leaves) and P1835 (with purple leaves). RESULTS: Genetic analysis indicated that the purple leaf trait is controlled by a single dominant gene, which we named BoPr...
March 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28287894/spinal-arachnoid-cysts-our-experience-and-review-of-literature
#8
Kanwaljeet Garg, Sachin Anil Borkar, Shashank Sharad Kale, Bhawani Shankar Sharma
OBJECTIVE: Arachnoid cysts are discrete pockets of CSF or CSF-like fluid found adjacent to normal CSF spaces, either spinal or cranial. Spinal arachnoid cysts (SAC) are most commonly extradural, however intradural or perineural are also described. METHODS: All patients admitted to our center and surgically treated with a diagnosis of SAC, were included in the study. The results were analyzed in terms of the clinical symptoms, location of cyst, surgical procedure performed and outcome following surgery...
April 2017: British Journal of Neurosurgery
https://www.readbyqxmd.com/read/28286475/neural-networks-for-time-perception-and-working-memory
#9
Sertaç Üstün, Emre H Kale, Metehan Çiçek
Time is an important concept which determines most human behaviors, however questions remain about how time is perceived and which areas of the brain are responsible for time perception. The aim of this study was to evaluate the relationship between time perception and working memory in healthy adults. Functional magnetic resonance imaging (fMRI) was used during the application of a visual paradigm. In all of the conditions, the participants were presented with a moving black rectangle on a gray screen. The rectangle was obstructed by a black bar for a time period and then reappeared again...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28285742/peripheral-quantitative-computed-tomography-pqct-measures-contribute-to-the-understanding-of-bone-fragility-in-older-patients-with-low-trauma-fracture
#10
Hongyuan Jiang, Christopher J Yates, Alexandra Gorelik, Ashwini Kale, Qichun Song, John D Wark
Dual-energy X-ray absorptiometry (DXA) as currently used has limitations in identifying patients with osteoporosis and predicting occurrence of fracture. We aimed to express peripheral quantitative computed tomography (pQCT) variables of patients with low-trauma fracture as T-scores by using T-score scales obtained from healthy young women, and to evaluate the potential clinical utility of pQCT for the assessment of bone fragility. Fracture patients were recruited from a fracture liaison service at the Royal Melbourne Hospital...
March 9, 2017: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/28283426/bringing-precision-medicine-to-the-management-of-bpsd
#11
EDITORIAL
Donovan T Maust, Helen C Kales
No abstract text is available yet for this article.
February 14, 2017: American Journal of Geriatric Psychiatry
https://www.readbyqxmd.com/read/28282404/characterization-of-bacterial-community-associated-with-phytoplankton-bloom-in-a-eutrophic-lake-in-south-norway-using-16s-rrna-gene-amplicon-sequence-analysis
#12
Niranjan Nitin Parulekar, Pandurang Kolekar, Andrew Jenkins, Synne Kleiven, Hans Utkilen, Anette Johansen, Sangeeta Sawant, Urmila Kulkarni-Kale, Mohan Kale, Mona Sæbø
Interactions between different phytoplankton taxa and heterotrophic bacterial communities within aquatic environments can differentially support growth of various heterotrophic bacterial species. In this study, phytoplankton diversity was studied using traditional microscopic techniques and the bacterial communities associated with phytoplankton bloom were studied using High Throughput Sequencing (HTS) analysis of 16S rRNA gene amplicons from the V1-V3 and V3-V4 hypervariable regions. Samples were collected from Lake Akersvannet, a eutrophic lake in South Norway, during the growth season from June to August 2013...
2017: PloS One
https://www.readbyqxmd.com/read/28274074/oral-carcinoma-cuniculatum-a-new-entity-in-the-clinicopathological-spectrum-of-oral-squamous-cell-carcinoma
#13
Uma Vasant Datar, Alka Kale, Deepa Mane
Carcinoma cuniculatum is principally recognized as a variant of carcinoma involving foot. The World Health Organization (WHO) recognizes Oral Carcinoma Cuniculatum (OCC) as a distinct and rare clinicopathological variant of Oral Squamous Cell Carcinoma (OSCC). OCC is confused clinically and histologically with Verrucous Carcinoma (VC) and is often misdiagnosed as either VC or OSCC. To best of our knowledge, till date, only 50 cases of this tumour have been reported in oral cavity (including the present case) and only limited number of cases have been reported from Indian subcontinent...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28270815/lanthionine-synthetase-c-like-2-modulates-immune-responses-to-influenza-virus-infection
#14
Andrew Leber, Josep Bassaganya-Riera, Nuria Tubau-Juni, Victoria Zoccoli-Rodriguez, Pinyi Lu, Victoria Godfrey, Shiv Kale, Raquel Hontecillas
Broad-based, host-targeted therapeutics have the potential to ameliorate viral infections without inducing antiviral resistance. We identified lanthionine synthetase C-like 2 (LANCL2) as a new therapeutic target for immunoinflammatory diseases. To examine the therapeutic efficacy of oral NSC61610 administration on influenza, we infected C57BL/6 mice with influenza A H1N1pdm virus and evaluated influenza-related mortality, lung inflammatory profiles, and pulmonary histopathology. Oral treatment with NSC61610 ameliorates influenza virus infection by down-modulating pulmonary inflammation through the downregulation of TNF-α and MCP-1 and reduction in the infiltration of neutrophils...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28269448/portable-closed-loop-optogenetic-stimulation-device
#15
Epsy S Edward, Abbas Z Kouzani, Rajas P Kale, Susannah J Tye
This paper presents a closed-loop optogenetic stimulation device to achieve online modulation of neurons. The device is designed to be mountable on small rodents in pre-clinical settings. Considering the size of rodents and the need for portability, a single-piece self-contained device is developed which allows real-time photostimulation based on detected neuronal states. It consists of three components: a neural recorder, a control algorithm, and an optogenetic stimulator. The neural recorder which is realized by analogue circuitry measures the neural signal...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28267121/current-techniques-for-postoperative-monitoring-of-microvascular-free-flaps
#16
Nasir Khatri, Shuhao Zhang, Santosh S Kale
Free tissue transfer (FTT) is used in patients with complicated reconstructive needs; it can provide stable wound coverage, improved aesthetic appearance, and restore functional deficits. Despite the high success rates of free flaps, vascular occlusion is a significant risk leading to flap failure. Many studies have demonstrated that the salvage rate for flaps is inversely related to the time between onset of a vascular problem and its surgical correction. As a result, ongoing postoperative monitoring of free flaps for adequate perfusion is imperative to allow timely and accurate diagnosis of vascular compromise...
March 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/28265052/amino-acid-oxidation-of-the-d1-and-d2-proteins-by-oxygen-radicals-during-photoinhibition-of-photosystem-ii
#17
Ravindra Kale, Annette E Hebert, Laurie K Frankel, Larry Sallans, Terry M Bricker, Pavel Pospíšil
The Photosystem II reaction center is vulnerable to photoinhibition. The D1 and D2 proteins, lying at the core of the photosystem, are susceptible to oxidative modification by reactive oxygen species that are formed by the photosystem during illumination. Using spin probes and EPR spectroscopy, we have determined that both O2(•-) and HO(•) are involved in the photoinhibitory process. Using tandem mass spectroscopy, we have identified a number of oxidatively modified D1 and D2 residues. Our analysis indicates that these oxidative modifications are associated with formation of HO(•) at both the Mn4O5Ca cluster and the nonheme iron...
March 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28263592/quantitative-and-atomic-scale-view-of-co-induced-pt-nanoparticle-surface-reconstruction-at-saturation-coverage-via-dft-calculations-coupled-with-in-situ-tem-and-ir
#18
Talin Avanesian, Sheng Dai, Matthew J Kale, George W Graham, Xiaoqing Pan, Phillip Christopher
Atomic-scale insights into how supported metal nanoparticles catalyze chemical reactions are critical for the optimization of chemical conversion processes. It is well-known that different geometric configurations of surface atoms on supported metal nanoparticles have different catalytic reactivity and that the adsorption of reactive species can cause reconstruction of metal surfaces. Thus, characterizing metallic surface structures under reaction conditions at atomic scale is critical for understanding reactivity...
March 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28261027/the-effect-of-vitamin-d-on-clinical-manifestations-and-activity-of-beh%C3%A3-et-s-disease
#19
Nurşad Aslan, Kamile Demirci, Tuba Güler, Fulya Dörtbaş, Ebru Kale
INTRODUCTION: Immune mechanisms have been implemented to have a role in the pathogenesis of Behçet's disease (BD) and vitamin D has been shown to have a regulatory role in the immune system function. AIM: To evaluate the vitamin D levels of BD patients and its relationship between clinical findings and disease activity of BD. MATERIAL AND METHODS: Sixty-eight patients with BD and 70 age- and sex-matched controls were examined retrospectively...
February 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28259697/antipsychotic-use-and-physical-morbidity-in-parkinson-disease
#20
Daniel Weintraub, Claire Chiang, Hyungjin Myra Kim, Jayne Wilkinson, Connie Marras, Barbara Stanislawski, Eugenia Mamikonyan, Helen C Kales
OBJECTIVE: To determine if antipsychotic (AP) use in Parkinson disease (PD) patients is associated with increased physical morbidity. METHODS: Veterans Health Administration data (1999-2010) was used to examine physical morbidity risk associated with AP use in idiopathic PD patients with stable recent physical health. We compared 180-day morbidity rates in patients initiating an AP with matched non-AP users who survived for 180 days (matched on age, sex, race, index year, presence and duration of dementia, PD duration, delirium, hospitalization, Charlson Comorbidity Index, and new non-psychiatric medications; covarying for psychosis)...
February 2, 2017: American Journal of Geriatric Psychiatry
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