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https://www.readbyqxmd.com/read/29322011/safety-evaluation-of-bon-sant%C3%A3-cleanser%C3%A2-polyherbal-in-male-wistar-rats-further-investigations-on-androgenic-and-toxicological-profile
#1
O Awodele, O E Kale, A O Odewabi, M Ekor, B A Salau, A O Adefule-Ositelu
Background: The global increase in acceptance and use of herbal remedies in recent times is still accompanied with poor knowledge of their potential adverse effects and the toxicological implications of their use are underestimated. Methods: Bon-santé Cleanser® (BSC), a polyherbal containing Anogeissus leiocarpus, Terminalia ivorensis, Massularia acuminate and Macuna pruriens, is an "energizer and hormone booster". We assessed the effect of BSC on reproductive function after administration for 60 days in male Wistar rats...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29316439/ribosomal-protein-s12e-has-a-distinct-function-in-cell-competition
#2
Abhijit Kale, Zhejun Ji, Marianthi Kiparaki, Jorge Blanco, Gerard Rimesso, Stephane Flibotte, Nicholas E Baker
Wild-type Drosophila cells can remove cells heterozygous for ribosomal protein mutations (known as "Minute" mutant cells) from genetic mosaics, a process termed cell competition. The ribosomal protein S12 was unusual because cells heterozygous for rpS12 mutations were not competed by wild-type, and a viable missense mutation in rpS12 protected Minute cells from cell competition with wild-type cells. Furthermore, cells with Minute mutations were induced to compete with one another by altering the gene dose of rpS12, eliminating cells with more rpS12 than their neighbors...
January 8, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29313452/fermentative-production-of-rhamnolipid-and-purification-by-adsorption-chromatography
#3
Jagruti Jadhav, Sruba Dutta, Sandeep Kale, Amit Prabhakar Pratap
Glycolipids are one of the major classes of biosurfactants in which the rhamnolipids are best studied. The present work investigates, optimization of inoculum age and batch time for maximizing the yield of rhamnolipid from Pseudomonas aeruginosa (MTCC 2453). The yield and titer of rhamnolipids were maximum in the fermentation batch with an inoculum age of 24 hours. Batch time studies were carried out on biomass production, rhamnolipid production and sunflower oil utilization. The maximum yield of rhamnolipid was achieved at 96 hours when the culture cells were in the late exponential/ early stationary phase...
January 9, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29310904/synthesis-and-characterization-of-vangabhasma
#4
Babita Kale, Nilima Rajurkar
BACKGROUND: Bhasmas are unique Ayurvedic organometallic preparations used for medicinal purpose. Quality of bhasma depends upon quality of starting materials, processing ingredients, meticulous trituration and heating cycle. In Ayurveda, Vanga bhasma is traditional Indian medicine which is an organometallic preparation treated with plant extract. It is especially used in the treatments of diseases related to gastrointestinal tract and genitor urinary system. However detailed characterization studies after synthesis are important which shows authenticity of product...
January 5, 2018: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29306822/celecoxib-a-cyclooxygenase-2-inhibitor-offers-chemoprevention-against-reproductive-and-neurobehavioural-abnormalities-induced-by-atrazine-in-male-wistar-rats
#5
O E Kale, T O Oyesola, F S Raji
The cyclooxygenase-2/prostanoid pathway (COX-2) serves as a potential therapeutic target in various pathological conditions. Thus, the modulatory effect of celecoxib (CXB), a COX-2 inhibitor, in atrazine-induced toxicity was investigated. Five groups (n = 6 rats per group) of adult male Wistar rats received corn oil (2 ml/kg), atrazine (ATZ, 300 mg/kg) and CXB (5.7 mg/kg) respectively and their combinations via the oral route. Results obtained showed reduced (p < 0.05) sperm motility (25.8%) and counts (27...
December 30, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29289736/systematic-magnetic-fluid-hyperthermia-studies-of-carboxyl-functionalized-hydrophilic-superparamagnetic-iron-oxide-nanoparticles-based-ferrofluids
#6
Ganeshlenin Kandasamy, Atul Sudame, Piyush Bhati, Anindita Chakrabarty, S N Kale, Dipak Maity
We have systematically studied heating efficiencies (via specific absorption rate-SAR/intrinsic loss power-ILP) of carboxyl (terephthalic acid-TA) functionalized hydrophilic SPIONs based ferrofluids (with good biocompatibility/high magnetization) and influence of following key factors in magnetic fluid hyperthermia (MFH): (i) alternating magnetic fields (AMFs - H)/frequencies (f) - chosen below/above Hergt's biological safety limit, (ii) concentrations (0.5-8 mg/ml) and (iii) dispersion media (water, a cell-culture medium and triethylene glycol (TEG)) for in vitro cancer therapy...
December 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29288297/biomonitoring-of-polychlorinated-biphenyls-in-bavaria-germany-long-term-observations-and-standardization
#7
Roland Weber, Stefan Gonser, Jutta Köhler, Wolfgang Körner, Christine Herold, Roland Haag, Margit Krapp, Ludwig Peichl
In the 1980s, it was demonstrated that semi-volatile organic compounds (SVOCs) like polychlorinated biphenyls (PCBs) accumulate in plant leaves. Plants are at the base of the food chain, and therefore a starting point for transfer of PCBs to animals and related human exposure. For two decades, the Environment Agency of the German federal state of Bavaria (LfU) has been operating long-term monitoring stations to measure the impact of organic air pollutants. Standardized ryegrass, curly kale, and spruce needles are used as bioindicators for the atmospheric entries of PCBs into vegetation...
December 29, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29278993/adherence-to-gold-guidelines-in-the-inpatient-copd-population
#8
Melissa Lipari, Amber Lanae Smith, Pramodini B Kale-Pradhan, Sheila M Wilhelm
PURPOSE: The Global Initiative for Chronic Obstructive Lung Disease guidelines provide recommendations to manage chronic obstructive lung disease (COPD) exacerbations. This study assessed the management of inpatient COPD exacerbations at an urban teaching hospital. METHODS: A retrospective cohort analysis of adults admitted between December 2010 and August 2012 with a COPD exacerbation was conducted. Patient demographics, length of stay (LOS), Charlson comorbidity score, inpatient pulmonary medications, and 30-day readmission were collected...
January 1, 2017: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/29274886/establishment-of-human-ipsc-line-nccsi003-a-from-cd34-cells-of-peripheral-blood-collected-during-apheresis-of-healthy-donor-from-indian-ethnicity
#9
Sophia Fernandes, Shruti Tembe, Prajakta Shinde, Sameer Melinkeri, Vaijayanti Kale, Lalita Limaye
We present generation of iPSCs from CD34+ cells isolated from peripheral blood, collected during apheresis of a healthy female individual. We nucleofected the CD34+cells by episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative mutation in p53). The resultant colonies showed cobble-stone appearance and stained positive for alkaline phosphatase. The colonies demonstrated presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells subsequently during passages as assessed by PCR...
December 20, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29260132/clinicopathological-analysis-of-appendiceal-mucinous-tumors-a-single-center-experience
#10
Arif Emre, Mehmet Sertkaya, İlhami Taner Kale
Objective: Appendiceal mucinous tumors can be encountered in four different types. The clinical approach varies according to these types and the severity of the present disease. We aimed to share clinical, radiological, and pathological features and surgical options of the patients diagnosed with mucinous tumors at our center. Material and Methods: Between August 2009 and March 2016, 757 patients underwent appendectomy for presumed diagnosis of acute appendicitis at the Department of Surgery, Kahramanmaraş Sütçü İmam University School of Medicine, Kahramanmaraş, Turkey...
2017: Turkish Journal of Surgery
https://www.readbyqxmd.com/read/29249636/anti-hyperglycemic-and-anti-hyperlipidaemic-effect-of-arjunarisht-in-high-fat-fed-animals
#11
Sushant A Shengule, Sanjay Mishra, Kalpana Joshi, Kishori Apte, Dada Patil, Prathmesh Kale, Tejas Shah, Mandavi Deshpande, Amrutesh Puranik
BACKGROUND: Arjunarisht (AA), a formulation used as cardiotonic is a hydroalcoholic formulation of Terminalia arjuna (Roxb.) Wight and Arn. (TA) belonging to family Combretaceae. OBJECTIVE: To evaluate the anti-hyperglycemic and anti-hyperlipidemic effect of Arjunarisht on high-fat diet fed animals. MATERIALS AND METHODS: High-fat diet fed (HFD) Wistar rats were randomly divided into three groups and treated with phytochemically standardized Arjunarisht (1...
December 14, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29248868/sleep-architecture-and-eeg-power-spectra-in-recently-detoxified-alcohol-dependent-patients
#12
Lokesh Kumar Singh, S Haque Nizamie, Sai Krishna Tikka
OBJECTIVES: Persistent sleep abnormalities during abstinence are a harbinger for relapse in patients with chronic alcohol dependence. The present study aimed to compare polysomnography (PSG) data between 'recently detoxified' patients with chronic alcohol dependence and healthy controls. METHODS: Both conventional sleep architectural and power spectral analyses were conducted. Twenty subjects in each of the groups were enrolled. A 2 nights' sleep (first-habituation and second-experimental) PSG data was collected...
December 9, 2017: Asian Journal of Psychiatry
https://www.readbyqxmd.com/read/29234083/nod2-is-required-for-the-early-innate-immune-clearance-of-acinetobacter-baumannii-from-the-lungs
#13
Sandeep D Kale, Neha Dikshit, Pankaj Kumar, Vanniarajan Balamuralidhar, Hanif Javanmard Khameneh, Najib Bin Abdul Malik, Tse Hsien Koh, Gladys Gek Yen Tan, Thuan Tong Tan, Alessandra Mortellaro, Bindu Sukumaran
Acinetobacter baumannii (A. baumannii) is a significant cause of severe nosocomial pneumonia in immunocompromised individuals world-wide. With limited treatment options available, a better understanding of host immnity to A. baumannii infection is critical to devise alternative control strategies. Our previous study has identified that intracellular Nod1/Nod2 signaling pathway is required for the immune control of A. baumannii in airway epithelial cells in vitro. In the current study, using Nod2-/- mice and an in vivo sublethal model of pulmonary infection, we show that Nod2 contributes to the early lung defense against A...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29230885/intercellular-transfer-of-microvesicles-from-young-mesenchymal-stromal-cells-rejuvenates-aged-murine-hematopoietic-stem-cells
#14
Rohan Kulkarni, Manmohan Bajaj, Suprita Ghode, Sapana Jalnapurkar, Lalita Limaye, Vaijayanti Kale
Donor age is one of the major concerns in Bone Marrow Transplantation, as the aged hematopoietic stem cells (HSCs) fail to engraft efficiently. Here, using murine system we show that a brief interaction of aged HSCs with young mesenchymal stromal cells (MSCs) rejuvenates them and restores their functionality via inter-cellular transfer of microvesicles (MVs) containing autophagy-related mRNAs. Importantly, we show that MSCs gain activated AKT signaling as a function of aging. Activated AKT reduces the levels of autophagy-related mRNAs in their MVs, and partitions miR-17 and miR-34a into their exosomes, which upon transfer into HSCs down-regulate their autophagy-inducing mRNAs...
December 12, 2017: Stem Cells
https://www.readbyqxmd.com/read/29230862/patient-perspectives-on-loss-of-local-obstetrical-services-in-rural-northern-minnesota
#15
Jennifer Pearson, Kale Siebert, Samantha Carlson, Nathan Ratner
BACKGROUND: Obstetrical care has been declining in rural communities. We examined patient choices and perspectives from two rural northern Minnesota communities who lost their local obstetrical services in July 2015. Our purpose was to characterize obstetrical use patterns through the years leading to and following the closure and to explore the effects of the closure on these communities. METHODS: Information introducing the project and providing access to the survey was mailed to women who received prenatal care in the communities of interest...
December 12, 2017: Birth
https://www.readbyqxmd.com/read/29220458/allerbase-a-comprehensive-allergen-knowledgebase
#16
Kiran Kadam, Rajiv Karbhal, V K Jayaraman, Sangeeta Sawant, Urmila Kulkarni-Kale
http://bioinfo.net.in/AllerBase/Home.html.
January 1, 2017: Database: the Journal of Biological Databases and Curation
https://www.readbyqxmd.com/read/29214145/bitter-orange-citrus-aurantium-l-extract-subchronic-90-day-safety-study-in-rats
#17
N S Deshmukh, S J Stohs, C C Magar, A Kale, B Sowmya
Bitter orange (Citrus aurantium L.) extracts are widely used in dietary supplements and bitter oranges are used in various juices and food products. p-Synephrine, the primary active constituent, comprises approximately 90% of total protoalkaloids. This study, performed per OECD 408 guidance, examined the 90-day subchronic safety/toxicity of an extract standardized to 50% p-synephrine at doses of 100, 300 and 1000 mg/kg/day to male and female rats. No adverse effects were observed with respect to any of the observed parameters of clinical signs, functional observations of sensory reactivity, grip strength and motor activity, ophthalmology, body weights, hematology, food consumption, urinalysis, organ weights, as well as gross and microscopic pathology at termination at any of the doses in either sex...
2017: Toxicology Reports
https://www.readbyqxmd.com/read/29213115/modulation-of-immune-signaling-and-metabolism-highlights-host-and-fungal-transcriptional-responses-in-mouse-models-of-invasive-pulmonary-aspergillosis
#18
Shiv D Kale, Tariq Ayubi, Dawoon Chung, Nuria Tubau-Juni, Andrew Leber, Ha X Dang, Saikumar Karyala, Raquel Hontecillas, Christopher B Lawrence, Robert A Cramer, Josep Bassaganya-Riera
Incidences of invasive pulmonary aspergillosis, an infection caused predominantly by Aspergillus fumigatus, have increased due to the growing number of immunocompromised individuals. While A. fumigatus is reliant upon deficiencies in the host to facilitate invasive disease, the distinct mechanisms that govern the host-pathogen interaction remain enigmatic, particularly in the context of distinct immune modulating therapies. To gain insights into these mechanisms, RNA-Seq technology was utilized to sequence RNA derived from lungs of 2 clinically relevant, but immunologically distinct murine models of IPA on days 2 and 3 post inoculation when infection is established and active disease present...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211204/pragmatic-criteria-of-the-definition-of-neonatal-near-miss-a-comparative-study
#19
Pauline Lorena Kale, Maria Helena Prado de Mello Jorge, Ruy Laurenti, Sandra Costa Fonseca, Kátia Silveira da Silva
OBJECTIVE: The objective of this study was to test the validity of the pragmatic criteria of the definitions of neonatal near miss, extending them throughout the infant period, and to estimate the indicators of perinatal care in public maternity hospitals. METHODS: A cohort of live births from six maternity hospitals in the municipalities of São Paulo, Niterói, and Rio de Janeiro, Brazil, was carried out in 2011. We carried out interviews and checked prenatal cards and medical records...
December 4, 2017: Revista de Saúde Pública
https://www.readbyqxmd.com/read/29204989/effects-of-glucose-and-gibberellic-acid-on-glucosinolate-content-and-antioxidant-properties-of-chinese-kale-sprouts
#20
Hui-Ying Miao, Meng-Yu Wang, Jia-Qi Chang, Han Tao, Bo Sun, Qiao-Mei Wang
Glucosinolates, anthocyanins, total phenols, and vitamin C, as well as antioxidant capacity, were investigated in Chinese kale sprouts treated with both glucose and gibberellic acid (GA3). The combination of 3% (0.03 g/ml) glucose and 5 μmol/L GA3 treatment was effective in increasing glucosinolate content while glucose or GA3 treatment alone did not influence significantly almost all individual glucosinolates or total glucosinolates. The total phenolic content and antioxidant activity of Chinese kale sprouts were enhanced by combined treatment with glucose and GA3, which could be useful in improving the main health-promoting compounds and antioxidant activity in Chinese kale sprouts...
2017: Journal of Zhejiang University. Science. B
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