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https://www.readbyqxmd.com/read/28411489/kale-borack1-is-involved-in-the-plant-response-to-salt-stress-and-peronospora-brassicae-gaumann
#1
Da-Hong Li, Fu-Jia Shen, Hong-Yan Li, Wei Li
The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, a kale (Brassica oleracea var. acephala f.tricolor) RACK1 gene (BoRACK1) was cloned by RT-PCR. The amino acid sequence of BoRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides...
March 24, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28409820/identification-and-control-of-novel-growth-inhibitors-in-fed-batch-cultures-of-chinese-hamster-ovary-cells
#2
Bhanu Chandra Mulukutla, Jaitashree Kale, Taylor Kalomeris, Michaela Jacobs, Gregory W Hiller
Chinese hamster ovary (CHO) cells in culture are known to consume large amounts of nutrients and divert most of them towards byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. Glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yielding higher peak cell densities and titers. Interestingly, even in such optimized cultures, cell growth slows and eventually stops, indicating the emergence of other factors that negatively affect cell growth...
April 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28408678/draft-genome-sequence-of-a-kale-brassica-oleracea-l-root-endophyte-pseudomonas-sp-strain-c9
#3
Aurelie Laugraud, Sandra Young, Emily Gerard, Maureen O'Callaghan, Steven Wakelin
Pseudomonas sp. strain C9 is a plant growth-promoting bacterium isolated from the root tissue of Brassica oleracea L. grown in soil from Marlborough, New Zealand. Its draft genome of 6,350,161 bp contains genes associated with plant growth promotion and biological control.
April 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28407911/characterization-of-phenolics-glucosinolates-and-antioxidant-activity-of-beverages-based-on-apple-juice-with-addition-of-frozen-and-freeze-dried-curly-kale-leaves-brassica-oleracea-l-var-acephala-l
#4
Róża Biegańska-Marecik, Elżbieta Radziejewska-Kubzdela, Roman Marecik
The aim of this study was to determine the polyphenols, glucosinolates and ascorbic acid content as well as antioxidant activity of beverages on the base of apple juice with addition of frozen and freeze-dried curly kale leaves. Upon enrichment with frozen (13%) and freeze-dried curly kale (3%), the naturally cloudy apple juice was characterized by an increase in phenolic compounds by 2.7 and 3.3-times, accordingly. The antioxidant activity of beverages with the addition of curly kale ranged from 6.6 to 9.4μmol Trolox/mL...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28406091/daptomycin-associated-eosinophilic-pneumonia-with-rechallenge-a-case-report%C3%A2
#5
Melanie Nickerson, Ashish Bhargava, Pramodini B Kale-Pradhan
PURPOSE: A case of daptomycin-associated acute eosinophilic pneumonia (AEP) with positive rechallenge is reported. SUMMARY: AEP associated with daptomycin is reported in the literature, and the product labeling contains a warning and precaution statement. Criteria for diagnosing daptomycin-induced AEP varies and generally includes bronchoalveolar lavage (BAL) eosinophils ≥ 25%. We report a case of a 70-year-old woman with cough, shortness of breath, and altered mental status who presented ~ 9 days after starting therapy with daptomycin to treat methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis...
April 13, 2017: International Journal of Clinical Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28396951/robot-assisted-laparoscopic-pectouteropexy-an-alternative-uterus-sparing-technique-for-pelvic-organ-prolapse-surgery
#6
Taner Usta, Tolga Karacan, Ahmet Kale, Sevgin Mutlu, Talha Tıryakı
The aim of this video is to demonstrate the alternative technique of robot-assisted laparoscopic pectouteropexy for uterus preservation in obese patients with pelvic organ prolapse. We present the case report of a 44-year-old patient with apical pelvic organ prolapse. A pelvic examination was performed during a Valsalva maneuver in the dorsal lithotomy position and in the standing position, and the patient was diagnosed with stage III apical prolapse in accordance with the Pelvic Organ Prolapse Quantification system of the International Continence Society (POP-Q: Аа -1, Ва 0, Вр 0, С +2)...
April 10, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28395807/generation-and-characterization-of-human-ipsc-line-from-cd34-cells-isolated-from-umbilical-cord-blood-belonging-to-indian-origin
#7
Sophia Fernandes, Manasi Talwadekar, Raju Agarwal, Velu Nair, Vaijayanti Kale, Lalita Limaye
We describe here the reprogramming of CD34(+) cells isolated from umbilical cord blood obtained after full term delivery of a healthy female child of Indian origin. The cells were nucleofected by episomal vectors expressing Oct4, Sox2, L-Myc, Klf4, Lin28 and p53DD (negative mutation in p53). Colonies were identified by alkaline phosphatase staining and characterized for expression of pluripotency markers at protein level by immunofluorescence, flow cytometry and at transcript level by PCR. Genomic stability of the cell line was checked by G-banded karyotype...
January 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28394016/integrative-intensive-retreats-for-veteran-couples-and-families-a-pilot-study-assessing-change-in-relationship-adjustment-posttraumatic-growth-and-trauma-symptoms
#8
J Kale Monk, Lauren M Oseland, Briana S Nelson Goff, Brian G Ogolsky, Kali Summers
We assessed a brief, systemic retreat-style intervention that was developed to address concerns about the utilization of services for veterans coping with traumatic stress. A total of 76 dyads (N = 152) were assessed before and after a 4-day retreat, which included psychoeducation, group and conjoint therapeutic sessions, and recreational relaxation components. Overall, participants reported a reduction in trauma symptoms, but only support persons experienced a significant increase in posttraumatic growth from pretest to posttest...
April 10, 2017: Journal of Marital and Family Therapy
https://www.readbyqxmd.com/read/28390604/protective-role-of-misoprostol-in-prevention-of-gentamicin-ototoxicity
#9
Murat Dogan, Halil Polat, Mehmet Yasar, Ali Bayram, Duran Karatas, İbrahim Hira, Ahmet Kale, Fatma Senel, İbrahim Özcan
OBJECTIVES: To demonstrate potential protective effect of misoprostol on cochlear toxicity caused by gentamicin with electrophysiological tests and histopathological studies. MATERIALS AND METHODS: The study included 80 ears of 40 rats with normal hearing threshold and DPOAE value in both ears. Animals were assigned into 4 groups. The rats were randomized into 4 groups. Group I (n = 10): Gentamicin, Group II (n = 10): Gentamicin plus misoprostol, Group III (n = 10): Saline; Group IV (n = 10): Misoprostol...
May 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28389490/diagnosis-and-repair-of-perineal-injuries-knowledge-before-and-after-expert-training-a-multicentre-observational-study-among-palestinian-physicians-and-midwives
#10
Kaled Zimmo, Katariina Laine, Åse Vikanes, Erik Fosse, Mohammed Zimmo, Hadil Ali, Ranee Thakar, Abdul H Sultan, Sahar Hassan
OBJECTIVE: To assess whether a 2 days training with experts teaching on diagnosis and repair of perineal injuries among Palestinian midwives and physicians could change their level of knowledge towards the correct diagnosis and treatment. STUDY DESIGN: Multicentre observational study. SETTING: Obstetric departments in 6 government Palestinian hospitals. PARTICIPANTS: All physicians and midwives who attended the training...
April 7, 2017: BMJ Open
https://www.readbyqxmd.com/read/28388313/arachidonic-acid-and-docosahexanoic-acid-enhance-platelet-formation-from-human-apheresis-derived-cd34-cells
#11
Ankita Dhenge, Kedar Limbkar, Sameer Melinkeri, Vaijayanti Prakash Kale, Lalita Limaye
An Aberration in megakaryopoiesis and thrombopoiesis, 2 important processes that maintain hemostasis, leads to thrombocytopenia. Though platelet transfusions are used to treat this condition, blood banks frequently face a shortage of platelets. Therefore, methods to generate platelets on a large scale are strongly desirable. However, to generate megakaryocytes (MKs) and platelets (PLTs) in numbers sufficient for clinical application, it is essential to understand the mechanism of platelet production and explore efficient strategies accordingly...
April 7, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28383117/an-alternative-prosthetic-approach-for-rehabilitation-of-two-edentulous-maxillectomy-patients-clinical-report
#12
Murat Yenisey, Şafak Külünk, Necati Kaleli
Obturator prosthesis is a common treatment method for maxillectomy patients for maintaining their oronasal separation and resuming their social lives. After tumor resection, the remaining anatomical structures have a significant effect on prosthesis retention. The present study describes the rehabilitation of two maxillectomy patients after cancer surgery using a prosthesis consisting of a denture and a special retentive obturator that is positioned in the anatomical undercuts of the nasal cavity. These patients have undergone total and subtotal maxillectomy surgery after the diagnosis of squamous cell carcinoma...
April 6, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28378144/development-and-genetic-characterization-of-a-novel-herbicide-imazethapyr-tolerant-mutant-in-rice-oryza-sativa-l
#13
D Shoba, M Raveendran, S Manonmani, S Utharasu, D Dhivyapriya, G Subhasini, S Ramchandar, R Valarmathi, Nitasha Grover, S Gopala Krishnan, A K Singh, Pawan Jayaswal, Prashant Kale, M K Ramkumar, S V Amitha Mithra, T Mohapatra, Kuldeep Singh, N K Singh, N Sarla, M S Sheshshayee, M K Kar, S Robin, R P Sharma
BACKGROUND: Increased water and labour scarcity in major rice growing areas warrants a shift towards direct seeded rice cultivation under which management of weeds is a major issue. Use of broad spectrum non-selective herbicides is an efficient means to manage weeds. Availability of rice genotypes with complete tolerance against broad-spectrum non-selective herbicides is a pre-requisite for advocating use of such herbicides. In the present study, we developed an EMS induced rice mutant, 'HTM-N22', exhibiting tolerance to a broad spectrum herbicide, 'Imazethapyr', and identified the mutations imparting tolerance to the herbicide...
December 2017: Rice
https://www.readbyqxmd.com/read/28374115/damage-control-surgery-in-intracerebral-hemorrhage-in-acute-leukemia-a-review-of-two-cases
#14
Jaskaran Singh, Kanwaljeet Garg, Rajeev Sharma, Sumit Sinha, Shashank Sharad Kale
INTRODUCTION: Intracerebral hemorrhage is the second most common cause of mortality (after infections) in acute leukemia and is responsible for approximately 20% of deaths due to acute leukemia. Management of intracerebral hemorrhage (ICH) is mostly conservative but there exist certain patients who need emergent surgery due to the poor Glasgow Coma Scale (GCS) despite their coagulopathic state. CASE REPORT: We present here two such cases which were successfully managed with decompressive craniectomy which was done as a damage control surgery thus stating the importance of surgical intervention in the management of acutely deteriorating patients rather than the commonly employed conservative management due to their coagulopathic state...
April 3, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28373524/firefighting-and-the-heart-implications-for-prevention
#15
EDITORIAL
Stefanos N Kales, Denise L Smith
No abstract text is available yet for this article.
April 4, 2017: Circulation
https://www.readbyqxmd.com/read/28365391/rational-approach-for-design-and-evaluation-of-anti-aggregation-agents-for-protein-stabilization-a-case-study-of-trehalose-phenylalaninate
#16
Smita S Kale, Krishnacharya G Akamanchi
The present work introduces new anti-aggregation agent (AAA) derived through our new approach for design and evaluation of anti-aggregation agent as a multi-purpose excipient to combat protein aggregation. Therapeutic proteins undergo aggregation due to even minor changes in environmental conditions like temperature, pH, shear and stress. Excipients play a vital role in prevention of aggregation. To stabilize a protein formulation different classes of excipients are used in combination after carefully selecting through laborious and time consuming trial and error experiments...
March 30, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28364706/biosensor-for-detection-of-dissolved-chromium-in-potable-water-a-review
#17
REVIEW
Puja Biswas, Abhinav Kumar Karn, P Balasubramanian, Paresh G Kale
The unprecedented deterioration rate of the environmental quality due to rapid urbanization and industrialization causes a severe global health concern to both ecosystem and humanity. Heavy metals are ubiquitous in nature and being used extensively in industrial processes, the exposure to excessive levels could alter the biochemical cycles of living systems. Hence the environmental monitoring through rapid and specific detection of heavy metal contamination in potable water is of paramount importance. Various standard analytical techniques and sensors are used for the detection of heavy metals include spectroscopy and chromatographic methods along with electrochemical, optical waveguide and polymer based sensors...
March 24, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28352892/unique-perforated-graphene-derived-from-bougainvillea-flowers-for-high-power-supercapacitors-a-green-approach
#18
Rajendra P Panmand, Purnima Patil, Yogesh Sethi, Sunil R Kadam, Milind V Kulkarni, Suresh W Gosavi, N R Munirathnam, Bharat B Kale
Herein, we demonstrated a green approach for the synthesis of high surface area (850 m(2) g(-1)) mesoporous perforated graphene (PG) from Bougainvillea flower for the first time using a template free single-step method. The existence of PG was confirmed by XRD, Raman spectroscopy, FESEM, and FETEM. Surprisingly, FETEM clearly showed 5-10 nm perforation on the graphene sheets. More significantly, these mesoporous perforated graphene sheets can be produced in large scale using the present green approach. Considering high surface area and unique perforated graphene architecture, these PGs were studied for supercapacitor applications in detail without any chemical or physical activation...
March 29, 2017: Nanoscale
https://www.readbyqxmd.com/read/28348614/influence-of-sodium-chloride-content-in-electrolyte-solution-on-electrochemical-impedance-measurements-of-human-dentin
#19
Aziza Eldarrat, Alec High, Girish Kale
BACKGROUND: The aim of this study was to investigate the influence of sodium chloride (NaCl) content in electrolyte solution on electrochemical impedance measurements of human dentin by employing electrochemical impedance spectroscopy. MATERIALS AND METHODS: Dentin samples were prepared from extracted molars. Electrochemical impedance measurements were carried out over a wide frequency range (0.01Hz-10MHz). After measurements, samples were characterized using scanning electron microscopy...
January 2017: Dental Research Journal
https://www.readbyqxmd.com/read/28343670/comparison-of-porcelain-bond-strength-of-different-metal-frameworks-prepared-by-using-conventional-and-recently-introduced-fabrication-methods
#20
Necati Kaleli, Duygu Saraç
STATEMENT OF PROBLEM: Most studies evaluating dental laser sintering systems have focused on the marginal accuracy of the restorations. However, the bond strength at the metal-ceramic interface is another important factor that affects the survival of restorations, and currently, few studies focus on this aspect. PURPOSE: The purpose of this in vitro study was to compare the porcelain bond strength of cobalt-chromium (Co-Cr) metal frameworks prepared by using the conventional lost-wax technique, milling, direct metal laser sintering (DMLS), and laser cusing, a direct process powder-bed system...
March 23, 2017: Journal of Prosthetic Dentistry
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