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https://www.readbyqxmd.com/read/27902440/synechococcus-diversity-along-a-trophic-gradient-in-the-osterseen-lake-district-bavaria
#1
Joachim Ruber, Franziska Bauer, Andrew Millard, Uta Raeder, Jürgen Geist, Katrin Zwirglmaier
Picocyanobacteria are important primary producers in freshwater, however there is still a knowledge gap regarding their diversity at the strain level. For this reason, the microbial diversity of four lakes with different trophic states was investigated by sequencing of the 16S rRNA gene using universal primers. The study was performed in selected lakes of the Osterseen Lake District, Germany, from 2012 to 2014 (Lake Schiffhuettensee: eutrophic; Lake Ostersee: meso-oligotrophic; Lake Groebensee: oligotrophic; Lake Lustsee: oligotrophic)...
November 1, 2016: Microbiology
https://www.readbyqxmd.com/read/27881349/-diagnosis-of-drowning-by-detecting-gyrb-and-16s-rrna-genes-of-aeromonas-hydrophila-using-pcr-capillary-electrophoresis
#2
Bai-Sheng Mai, Qu-Yi Xu, Chao Liu, Jian Zhao, Ya-Li Han
OBJECTIVE: To establish a method for diagnosis of freshwater drowning by amplifying gyrB and 16S rRNA genes of Aeromonas hydrophila using PCR coupled with capillary electrophoresis (CE). METHODS: DNA samples were extracted from human, 18 planktons (including Candida albicans, Aeromonas hydrophila, and 16 species of algae), and 30 cases of tissue samples (including the lung, liver, and kidney, all examined with microwave digestion-vacuum filtration-automated scanning electron microscopy) from human cadavers, including 28 freshwater drowning victims and 2 with natural death...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27871041/toxicity-of-aqueous-vanadium-to-zooplankton-and-phytoplankton-species-of-relevance-to-the-athabasca-oil-sands-region
#3
Stephanie Schiffer, Karsten Liber
Vanadium (V) is an abundant trace metal present in bitumen from the Athabasca Oil Sands (AOS) region in Alberta, Canada. The upgrading of bitumen can result in the production of large volumes of a carbonaceous material referred to as petroleum coke that contains V at elevated levels compared to the native bitumen. Previous studies have shown that coke has the capacity to leach ecotoxicologically relevant levels of V into water it contacts, yet limited data are available on the toxicity of aqueous V to planktonic organisms...
November 18, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27807262/non-reef-environments-impact-the-diversification-of-extant-jacks-remoras-and-allies-carangoidei-percomorpha
#4
Bruno Frédérich, Giuseppe Marramà, Giorgio Carnevale, Francesco Santini
Various factors may impact the processes of diversification of a clade. In the marine realm, it has been shown that coral reef environments have promoted diversification in various fish groups. With the exception of requiem sharks, all the groups showing a higher level of diversity in reefs than in non-reef habitats have diets based predominantly on plankton, algae or benthic invertebrates. Here we explore the pattern of diversification of carangoid fishes, a clade that includes numerous piscivorous species (e...
November 16, 2016: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/27779795/dna-extraction-from-benthic-cyanobacteria-comparative-assessment-and-optimisation
#5
Virginie Gaget, Angela Keulen, Melody Lau, Paul Monis, Justin D Brookes
AIMS: Benthic cyanobacteria produce toxic and odorous compounds similar to their planktonic counterparts, challenging the quality of drinking water supplies. The biofilm that benthic algae and other micro-organisms produce is a complex and protective matrix. Monitoring to determine the abundance and identification of cyanobacteria therefore relies on molecular techniques, with the choice of DNA isolation technique critical. This study investigated which DNA extraction method is optimal for DNA recovery in order to guarantee the best DNA yield for PCR-based analysis of benthic cyanobacteria...
October 25, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27779617/diversity-and-oceanic-distribution-of-prasinophytes-clade-vii-the-dominant-group-of-green-algae-in-oceanic-waters
#6
Adriana Lopes Dos Santos, Priscillia Gourvil, Margot Tragin, Mary-Hélène Noël, Johan Decelle, Sarah Romac, Daniel Vaulot
Prasinophytes clade VII is a group of pico/nano-planktonic green algae (division Chlorophyta) for which numerous ribosomal RNA (rRNA) sequences have been retrieved from the marine environment in the last 15 years. A large number of strains have also been isolated but have not yet received a formal taxonomic description. A phylogenetic analysis of available strains using both the nuclear 18S and plastidial 16S rRNA genes demonstrates that this group composes at least 10 different clades: A1-A7 and B1-B3. Analysis of sequences from the variable V9 region of the 18S rRNA gene collected during the Tara Oceans expedition and in the frame of the Ocean Sampling Day consortium reveal that clade VII is the dominant Chlorophyta group in oceanic waters, replacing Mamiellophyceae, which have this role in coastal waters...
October 25, 2016: ISME Journal
https://www.readbyqxmd.com/read/27759035/mdrna-seq-analysis-of-marine-microbial-communities-from-the-northern-red-sea
#7
Shengwei Hou, Ulrike Pfreundt, Dan Miller, Ilana Berman-Frank, Wolfgang R Hess
Metatranscriptomic differential RNA-Seq (mdRNA-Seq) identifies the suite of active transcriptional start sites at single-nucleotide resolution through enrichment of primary transcript 5' ends. Here we analyzed the microbial community at 45 m depth at Station A in the northern Gulf of Aqaba, Red Sea, during 500 m deep mixing in February 2012 using mdRNA-Seq and a parallel classical RNA-Seq approach. We identified promoters active in situ for five different pico-planktonic genera (the SAR11 clade of Alphaproteobacteria, Synechococcus of Cyanobacteria, Euryarchaeota, Thaumarchaeota, and Micromonas as an example for picoeukaryotic algae), showing the applicability of this approach to highly diverse microbial communities...
October 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27544677/what-underpins-the-trophic-networks-of-the-plankton-in-shallow-oxbow-lakes
#8
J Kosiba, E Wilk-Woźniak, W Krztoń, M Strzesak, A Pociecha, E Walusiak, K Pudaś, E Szarek-Gwiazda
The aim of this study was to determine the relationships in the microbial trophic network underpinning them about communities of plankton ciliates in shallow oxbow lakes of the Vistula River in southern Poland (Jeziorzany 1, Jeziorzany 2, Piekary, Tyniec). The plankton components (phytoplankton, ciliates, zooplankton) were grouped by dietary preference. The studied oxbows differed in physicochemical parameters and in phytoplankton. Cyanobacteria dominated in the total biomass of phytoplankton in the Tyniec oxbow, big green algae (>30 μm) in Piekary and Jeziorzany 1, and euglenoids in Jeziorzany 2 oxbow...
August 20, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27507040/lunar-influence-on-prey-availability-diet-shifts-and-niche-overlap-between-engraulidae-larvae-in-tropical-mangrove-creeks
#9
A R A Lima, M Barletta
The influence of the lunar cycle on prey availability, diet shifts and overlap between larval Anchovia clupeoides and Cetengraulis edentulus was evaluated in mangrove creeks of the Goiana Estuary. Copepod eggs were highly abundant in the first and last quarter, at the full moon and zoea of Ucides cordatus (Ocypodidae) in the new moon. The Engraulidae larvae fed on microcrustaceans, algae and early planktonic stages of benthic organisms. The relative importance of prey varied according to prey availability in all moon phases...
October 2016: Journal of Fish Biology
https://www.readbyqxmd.com/read/27506027/-community-structure-characteristics-of-diatom-in-reservoirs-located-in-the-south-of-jiangsu-province-china-and-its-control-factors
#10
Jie Ren, Tao Zhou, Guang-wei Zhu, Ying-wei Jin, Yang Cui, Hai Xu, Meng-yuan Zhu, Ming-fang Xia, Wei-min Chen
In order to understand the community structure characteristics of Bacillariophyta and its controlling factors in reservoirs located in the Southeast, China, in the geographic background of hills landscape and humid climate, 18 reservoirs were investigated in June, 2015, during the period with high risk of diatom bloom, covering water quality, and the community structures of phytoplankton. The correlations between Bacillariophyta and other planktons with nutrients, water depth, storage capacity, etc. were analyzed...
May 15, 2016: Huan Jing Ke Xue= Huanjing Kexue
https://www.readbyqxmd.com/read/27484944/multiple-regression-analysis-to-assess-the-role-of-plankton-on-the-distribution-and-speciation-of-mercury-in-water-of-a-contaminated-lagoon
#11
T Stoichev, E Tessier, D Amouroux, C M Almeida, M C P Basto, V M Vasconcelos
Spatial and seasonal variation of mercury species aqueous concentrations and distributions was carried out during six sampling campaigns at four locations within Laranjo Bay, the most mercury-contaminated area of the Aveiro Lagoon (Portugal). Inorganic mercury (IHg(II)) and methylmercury (MeHg) were determined in filter-retained (IHgPART, MeHgPART) and filtered (<0.45μm) fractions (IHg(II)DISS, MeHgDISS). The concentrations of IHgPART depended on site and on dilution with downstream particles. Similar processes were evidenced for MeHgPART, however, its concentrations increased for particles rich in phaeophytin (Pha)...
November 15, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27472028/effect-of-copper-contaminated-food-on-the-life-cycle-and-secondary-production-of-daphnia-laevis
#12
Giseli S Rocha, Alessandra E Tonietto, Ana T Lombardi, Maria da G G Melão
In aquatic environments, copper (Cu) plays important physiological roles in planktonic food chain, such as electron transfer in photosynthesis and constituting proteins that transport oxygen in some arthropods, while at higher concentrations it is toxic on these organisms and higher trophic levels. The combined effects of natural (e.g. volcanic activity) and anthropogenic sources (e.g. mining waste) contribute to the increase in copper pollution in different ecosystems and regions around the world. In the present study, we evaluated the bioaccumulation and effect of Cu on Raphidocelis subcapitata (freshwater algae), and the influence of Cu-contaminated food (algae) on Daphnia laevis (tropical cladoceran)...
November 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27441705/hydrological-controls-on-ecosystem-dynamics-in-lake-fryxell-antarctica
#13
Radu Herbei, Alexander L Rytel, W Berry Lyons, Diane M McKnight, Christopher Jaros, Michael N Gooseff, John C Priscu
The McMurdo Dry Valleys constitute the largest ice free area of Antarctica. The area is a polar desert with an annual precipitation of ∼ 3 cm water equivalent, but contains several lakes fed by glacial melt water streams that flow from four to twelve weeks of the year. Over the past ∼20 years, data have been collected on the lakes located in Taylor Valley, Antarctica as part of the McMurdo Dry Valley Long-Term Ecological Research program (MCM-LTER). This work aims to understand the impact of climate variations on the biological processes in all the ecosystem types within Taylor Valley, including the lakes...
2016: PloS One
https://www.readbyqxmd.com/read/27348854/polysaccharide-utilization-loci-of-bacteroidetes-from-two-contrasting-open-ocean-sites-in-the-north-atlantic
#14
Christin M Bennke, Karen Krüger, Lennart Kappelmann, Sixing Huang, Angélique Gobet, Margarete Schüler, Valérie Barbe, Bernhard M Fuchs, Gurvan Michel, Hanno Teeling, Rudolf I Amann
Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes-affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here we report on the analysis of further 174 fosmids from the same libraries. The combined, re-assembled dataset (226 contigs; 8.8 Mbp) suggests that planktonic Bacteroidetes at the oligotrophic southern station use more peptides and bacterial and animal polysaccharides, whereas Bacteroidetes at the polar station (East-Greenland Current) use more algal and plant polysaccharides...
June 27, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27337877/-temporal-and-spatial-distribution-of-environmental-factors-and-phytoplankton-during-algal-bloom-season-in-pengxi-river-three-gorges-reservoir
#15
Chuan Zhou, Jian-jun Yu, Li Fu, Yu-jie Cui, De-fu Liu, Wei Jiang, Douglas Haffner, Lei Zhang
Planktonic algae and Related Environmental Factors were monitored during the period of algal blooms season in 2014 (Spring April 17 and summer 27 July) in Pengxi river, Three Gorges Reservoir. Mathematical statistical tools were used to analyze the community structure of planktonic algae in Pengxi River with its environmental factors. The results of sampling in spring showed that except the estuary site, S1 and the site close by, S2, the waters stratified, but without epilimnion, only had metalimnion and hypolimnion; the upstream sites had larger temperature difference between water layers than did the downstream ones; from all the 8 sampling sites from estuary to upstream, water depth, and the temperature, pH, conductivity, dissolved oxygen, chlorophyll a, TN and TP of the surface layer (0-0...
March 15, 2016: Huan Jing Ke Xue= Huanjing Kexue
https://www.readbyqxmd.com/read/27057086/chlorophyll-catabolites-in-conditioned-media-of-green-microalga-desmodesmus-subspicatus
#16
Krzysztof Grabski, Natalia Baranowski, Joanna Skórko-Glonek, Zbigniew Tukaj
Although the appearance of coloured chlorophyll degradation products of higher plants is well known, knowledge about such compounds produced and released particularly by planktonic algae is still limited. Colourless conditioned media (CM) obtained from autotrophic cultures of unicellular green alga Desmosdemus subspicatus turn red after acidification. The accumulation of red pigments in the medium and the growth rate of algae were inversely correlated. The red, crude solution isolated from CM by dialysis and ion exchange chromatography, and next purified by means of high-performance liquid chromatography, appeared to be a mixture of three compounds with characteristic UV/VIS absorption maxima near 330 and 505 nm...
2016: Journal of Applied Phycology
https://www.readbyqxmd.com/read/27054497/recurring-patterns-in-bacterioplankton-dynamics-during-coastal-spring-algae-blooms
#17
Hanno Teeling, Bernhard M Fuchs, Christin M Bennke, Karen Krüger, Meghan Chafee, Lennart Kappelmann, Greta Reintjes, Jost Waldmann, Christian Quast, Frank Oliver Glöckner, Judith Lucas, Antje Wichels, Gunnar Gerdts, Karen H Wiltshire, Rudolf I Amann
A process of global importance in carbon cycling is the remineralization of algae biomass by heterotrophic bacteria, most notably during massive marine algae blooms. Such blooms can trigger secondary blooms of planktonic bacteria that consist of swift successions of distinct bacterial clades, most prominently members of the Flavobacteriia, Gammaproteobacteria and the alphaproteobacterial Roseobacter clade. We investigated such successions during spring phytoplankton blooms in the southern North Sea (German Bight) for four consecutive years...
2016: ELife
https://www.readbyqxmd.com/read/26929141/phylogenetic-diversity-and-biogeography-of-the-mamiellophyceae-lineage-of-eukaryotic-phytoplankton-across-the-oceans
#18
Adam Monier, Alexandra Z Worden, Thomas A Richards
High-throughput diversity amplicon sequencing of marine microbial samples has revealed that members of the Mamiellophyceae lineage are successful phytoplankton in many oceanic habitats. Indeed, these eukaryotic green algae can dominate the picoplanktonic biomass, however, given the broad expanses of the oceans, their geographical distributions and the phylogenetic diversity of some groups remain poorly characterized. As these algae play a foundational role in marine food webs, it is crucial to assess their global distribution in order to better predict potential changes in abundance and community structure...
August 2016: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/26914213/toxic-diatom-aldehydes-affect-defence-gene-networks-in-sea-urchins
#19
Stefano Varrella, Giovanna Romano, Susan Costantini, Nadia Ruocco, Adrianna Ianora, Matt G Bentley, Maria Costantini
Marine organisms possess a series of cellular strategies to counteract the negative effects of toxic compounds, including the massive reorganization of gene expression networks. Here we report the modulated dose-dependent response of activated genes by diatom polyunsaturated aldehydes (PUAs) in the sea urchin Paracentrotus lividus. PUAs are secondary metabolites deriving from the oxidation of fatty acids, inducing deleterious effects on the reproduction and development of planktonic and benthic organisms that feed on these unicellular algae and with anti-cancer activity...
2016: PloS One
https://www.readbyqxmd.com/read/26909430/when-is-a-type-iii-functional-response-stabilizing-theory-and-practice-of-predicting-plankton-dynamics-under-enrichment
#20
Wojciech Uszko, Sebastian Diehl, Nadine Pitsch, Kathrin Lengfellner, Thomas Müller
The curvature of generalized Holling type functional response curves is controlled by a shape parameter b yielding hyperbolic type II (b = 1) to increasingly sigmoid type III (b > 1) responses. Empirical estimates of b vary considerably across taxa. Larger consumer-resource body mass ratios have been suggested to generate more pronounced type III responses and therefore to promote dynamic stability. The dependence of consumer- resource stability on b has, however, not been systematically explored, and the accurate empirical determination of b is challenging...
December 2015: Ecology
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