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https://www.readbyqxmd.com/read/28607240/social-impacts-on-adult-use-of-tobacco-findings-from-the-international-tobacco-control-project-india-wave-1-survey
#1
Cecily S Ray, M S Pednekar, P C Gupta, M Bansal-Travers, Ack Quah, G T Fong
BACKGROUND: Social impacts on tobacco use have been reported but not well quantified. This study investigated how strongly the use of smoked and smokeless tobacco may be influenced by other users who are close to the respondents. METHODS: The International Tobacco Control Project (TCP), India, used stratified multistage cluster sampling to survey individuals aged ≥15 years in four areas of India about their tobacco use and that of their close associates. The present study used logistic regression to calculate odds ratios (ORs) for tobacco use for each type of close associate...
September 2016: WHO South-East Asia Journal of Public Health
https://www.readbyqxmd.com/read/28604643/an-energy-aware-hybrid-arq-scheme-with-multi-acks-for-data-sensing-wireless-sensor-networks
#2
Jinhuan Zhang, Jun Long
Wireless sensor networks (WSNs) are one of the important supporting technologies of edge computing. In WSNs, reliable communications are essential for most applications due to the unreliability of wireless links. In addition, network lifetime is also an important performance metric and needs to be considered in many WSN studies. In the paper, an energy-aware hybrid Automatic Repeat-reQuest protocol (ARQ) scheme is proposed to ensure energy efficiency under the guarantee of network transmission reliability. In the scheme, the source node sends data packets continuously with the correct window size and it does not need to wait for the acknowledgement (ACK) confirmation for each data packet...
June 12, 2017: Sensors
https://www.readbyqxmd.com/read/28594177/a-versatile-toolbox-for-the-control-of-protein-levels-using-n-%C3%AE%C2%B5-acetyl-l-lysine-dependent-amber-suppression
#3
Wolfram Volkwein, Christopher Maier, Ralph Krafczyk, Kirsten Jung, Jürgen Lassak
The analysis of the function of essential genes in vivo depends on the ability to experimentally modulate levels of their protein products. Current methods to address this are based on transcriptional or post-transcriptional regulation of mRNAs but approaches based on the exploitation of translational regulation have so far been neglected. Here we describe a toolbox, based on amber suppression in the presence of Nε-acetyl-L-lysine (AcK), for translational tuning of protein output. We chose the highly sensitive luminescence system LuxCDABE as a reporter and incorporated a UAG stop codon into the gene for the reductase subunit LuxC...
June 8, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28593724/a-new-strategy-for-creating-noncanonical-amino-acid-dependent-organisms
#4
Weimin Xuan, Peter G Schultz
The use of noncanonical amino acids (ncAAs) to control an organism's viability provides a strategy for the development of conditional 'kill switches' for live vaccines or engineered human cells. Here we report an approach inspired by the posttranslational acetylation/deacetylation of lysine residues, in which a protein encoded by a gene with an in-frame nonsense codon at an essential lysine can be expressed in its native state only upon genetic incorporation of AcK, and subsequent enzymatic deacetylation in the host cell...
June 7, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28539360/a-dock-and-coalesce-mechanism-driven-by-hydrophobic-interactions-governs-cdc42-binding-with-its-effector-protein-ack
#5
George J N Tetley, Helen R Mott, R Neil Cooley, Darerca Owen
Cdc42 is a Rho-family small G protein that has been widely studied for its role in controlling the actin cytoskeleton and plays a part in several potentially oncogenic signalling networks. Similar to most other small G proteins, Cdc42 binds to many downstream effector proteins to elicit its cellular effects. These effector proteins all engage the same face of Cdc42, the conformation of which is governed by the activation state of the G protein. Previously, the importance of individual residues in conferring binding affinity has been explored for residues within Cdc42 for three of its CRIB effectors, activated Cdc42 kinase (ACK), p21-activated kinase (PAK), and Wiskott-Aldrich syndrome protein (WASP)...
May 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28505492/comprehensive-evaluation-of-scfa-production-in-the-intestinal-bacteria-regulated-by-berberine-using-gas-chromatography-combined-with-polymerase-chain-reaction
#6
Lu-Lu Wang, Hui-Hui Guo, Shuai Huang, Chen-Lin Feng, Yan-Xing Han, Jian-Dong Jiang
Short-chain fatty acids (SCFAs) of intestine microbial have caught accumulating attention for their beneficial effects on human health. Botanic compounds with low bioavailability such as berberine (BBR) and resveratrol might interact with intestinal microbial ecosystem and promote gut bacteria to produce SCFA, which contribute to their biological effects. In the present study, a comprehensive assay system was built to detect SCFAs production in intestinal bacteria, in which stringent anaerobic culture was applied for in vitro bacterial fermentation, followed by direct-injection GC detection (chemical detection) in combination with real time polymerase chain reaction (RT-PCR, biological detection)...
July 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28455447/acetylation-of-lysine-%C3%AF%C2%B5-amino-groups-regulates-aminoacyl-trna-synthetase-activity-in-escherichia-coli
#7
Qing Ye, Quan-Quan Ji, Wei Yan, Fang Yang, En-Duo Wang
Previous proteomic analyses have shown that aminoacyl-tRNA synthetases (aaRSs) in many organisms can be modified by acetylation of lysine (Lys). In this present study, leucyl-tRNA synthetase and arginyl-tRNA synthetase from Escherichia coli (EcLeuRS and EcArgRS) were overexpressed and purified, and found to be acetylated on Lys residues by mass spectrometry (MS). Glutamine (Gln) scanning mutagenesis revealed that Lys619, Lys624 and Lys809 in EcLeuRS and Lys126 and Lys408 in EcArgRS might play important roles in enzyme activity...
April 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28450293/extracellular-cyclophilin-a-especially-acetylated-causes-pulmonary-hypertension-by-stimulating-endothelial-apoptosis-redox-stress-and-inflammation
#8
Chao Xue, Mark Sowden, Bradford C Berk
OBJECTIVE: Oxidative stress and inflammation play key roles in the development of pulmonary arterial hypertension (PAH). Cyclophilin A (CypA) is secreted in response to oxidative stress and promotes inflammation and cardiovascular disease. Endothelial cell (EC) dysfunction is an early event in the pathogenesis of PAH. We evaluated the role of extracellular CypA in PAH and compared the effects of acetylated CypA (AcK-CypA, increased by oxidative stress) and CypA on EC dysfunction. APPROACH AND RESULTS: In transgenic mice that express high levels of CypA in EC specifically, a PAH phenotype was observed at 3 months including increased right ventricular systolic pressure, α-smooth muscle actin expression in small arterioles, and CD45-positive cells in the lungs...
June 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28293295/electrode-assisted-acetoin-production-in-a-metabolically-engineered-escherichia-coli-strain
#9
Andreas H Förster, Sebastian Beblawy, Frederik Golitsch, Johannes Gescher
BACKGROUND: This paper describes the metabolic engineering of Escherichia coli for the anaerobic fermentation of glucose to acetoin. Acetoin has well-established applications in industrial food production and was suggested to be a platform chemical for a bio-based economy. However, the biotechnological production is often hampered by the simultaneous formation of several end products in the absence of an electron acceptor. Moreover, typical production strains are often potentially pathogenic...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28131378/multiplex-pcr-based-identification-of-streptococcus-canis-streptococcus-zooepidemicus-and-streptococcus-dysgalactiae-subspecies-from-dogs
#10
M Moriconi, E Acke, D Petrelli, S Preziuso
Streptococcus canis (S. canis), Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) and Streptococcus dysgalactiae subspecies (S. dysgalactiae subspecies) are β-haemolytic Gram positive bacteria infecting animals and humans. S. canis and S. zooepidemicus are considered as two of the major zoonotic species of Streptococcus, while more research is needed on S. dysgalactiae subspecies bacteria. In this work, a multiplex-PCR protocol was tested on strains and clinical samples to detect S. canis, S. dysgalactiae subspecies and S...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28130304/ccpa-and-cody-coordinate-acetate-metabolism-in-streptococcus-mutans
#11
Jeong Nam Kim, Robert A Burne
In the dental caries pathogen Streptococcus mutans, phosphotransacetylase (Pta) and acetate kinase (Ack) convert pyruvate into acetate with the concomitant generation of ATP. The genes for this pathway are tightly regulated by multiple environmental and intracellular inputs, but the basis for differential expression of the genes for Pta and Ack in S. mutans had not been investigated. Here, we show that inactivation in S. mutans of ccpA or codY reduced the activity of the ackA promoter, whereas a ccpA mutant displayed elevated pta promoter activity...
April 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28113691/estimation-and-lqg-control-over-unreliable-network-with-acknowledgment-randomly-lost
#12
Hong Lin, Hongye Su, Peng Shi, Renquan Lu, Zheng-Guang Wu
In this paper, we study the state estimation and optimal control [i.e., linear quadratic Gaussian (LQG) control] problems for networked control systems in which control inputs, observations, and packet acknowledgments (ACKs) are randomly lost. The packet ACK is a signal that is transmitted from the actuator to notice the estimator the occurence of control packet loss. For such systems, we obtain the optimal estimator, which is consisted of exponentially increasing terms. For the solvability of the LQG problem, we come to a conclusion that in general even the optimal LQG control exists, it is impossible and unnecessary to be obtained as its calculation is not only technically difficult but also computationally prohibitive...
September 13, 2016: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28088294/suitability-of-small-diagnostic-peripheral-blood-samples-for-cell-therapy-studies
#13
Coralea Stephanou, Panayiota Papasavva, Myria Zachariou, Petros Patsali, Marilena Epitropou, Petros Ladas, Ruba Al-Abdulla, Soteroulla Christou, Michael N Antoniou, Carsten W Lederer, Marina Kleanthous
BACKGROUND AIMS: Primary hematopoietic stem and progenitor cells (HSPCs) are key components of cell-based therapies for blood disorders and are thus the authentic substrate for related research. We propose that ubiquitous small-volume diagnostic samples represent a readily available and as yet untapped resource of primary patient-derived cells for cell- and gene-therapy studies. METHODS: In the present study we compare isolation and storage methods for HSPCs from normal and thalassemic small-volume blood samples, considering genotype, density-gradient versus lysis-based cell isolation and cryostorage media with different serum contents...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/28075347/sdtcp-towards-datacenter-tcp-congestion-control-with-sdn-for-iot-applications
#14
Yifei Lu, Zhen Ling, Shuhong Zhu, Ling Tang
The Internet of Things (IoT) has gained popularity in recent years. Today's IoT applications are now increasingly deployed in cloud platforms to perform Big Data analytics. In cloud data center networks (DCN), TCP incast usually happens when multiple senders simultaneously communicate with a single receiver. However, when TCP incast happens, DCN may suffer from both throughput collapse for TCP burst flows and temporary starvation for TCP background flows. In this paper, we propose a software defined network (SDN)-based TCP congestion control mechanism, referred to as SDTCP, to leverage the features, e...
January 8, 2017: Sensors
https://www.readbyqxmd.com/read/28036001/tple-a-reliable-data-delivery-scheme-for-on-road-wsn-traffic-monitoring
#15
Rui Wang, Fei Chang, Suli Ren
In an on-road environment, motor-engines severely disturb the wireless link of a sensor node, leading to high package loss rate, high delivery delay, and poor radio communication quality. The existing data delivery mechanisms, such as the ACK-based retransmission mechanism and window-based link quality estimation mechanism, could not handle these challenges well. To solve this challenge, we propose a Target-Prediction-based Link quality Estimation scheme (TPLE) to realize high quality data delivery in an on-road environment...
December 27, 2016: Sensors
https://www.readbyqxmd.com/read/27860343/isolation-of-campylobacter-spp-from-client-owned-dogs-and-cats-and-retail-raw-meat-pet-food-in-the-manawatu-new-zealand
#16
K Bojanić, A C Midwinter, J C Marshall, L E Rogers, P J Biggs, E Acke
Campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. The disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. The prevalence of Campylobacter spp. in dogs and cats varies widely, and data on New Zealand pets are limited. This study aimed to investigate the prevalence of Campylobacter spp. in dogs, cats and retail raw meat pet food products in New Zealand and to characterize Campylobacter jejuni isolates using multilocus sequence typing (MLST)...
November 12, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27738720/efficient-multi-enzyme-catalyzed-cdp-choline-production-driven-by-an-atp-donor-module
#17
Yingmiao Liu, Junzhi Wang, Chongmao Xu, Yong Chen, Junjie Yang, Dong Liu, Huanqing Niu, Yu Jiang, Sheng Yang, Hanjie Ying
Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP kinase (NDK), choline phosphate cytidylyltransferase (CCT), and choline kinase (CKI) to produce CDP-choline from CMP and choline chloride. Within 1 h, 49 mM CDP-choline was produced, for a molar yield of 89...
February 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27669243/gateway-assisted-retransmission-for-lightweight-and-reliable-iot-communications
#18
Hui-Ling Chang, Cheng-Gang Wang, Mong-Ting Wu, Meng-Hsun Tsai, Chia-Ying Lin
Message Queuing Telemetry Transport for Sensor Networks (MQTT-SN) and Constrained Application Protocol (CoAP) are two protocols supporting publish/subscribe models for IoT devices to publish messages to interested subscribers. Retransmission mechanisms are introduced to compensate for the lack of data reliability. If the device does not receive the acknowledgement (ACK) before retransmission timeout (RTO) expires, the device will retransmit data. Setting an appropriate RTO is important because the delay may be large or retransmission may be too frequent when the RTO is inappropriate...
September 22, 2016: Sensors
https://www.readbyqxmd.com/read/27632366/evaluation-and-comparison-of-vitamin-d-responsive-gene-expression-in-ovine-canine-and-equine-kidney
#19
Sara Azarpeykan, Keren E Dittmer, Jonathan C Marshall, Kalyani C Perera, Erica K Gee, Els Acke, Keith G Thompson
The aim of this study was to determine the relative abundance and relationship of vitamin D responsive and calcium transporting transcripts (TRPV5, TRPV6, calD9k, calD28k, PMCA, NCX1, CYP27B1, CYP24A1, and VDR) in ovine, canine and, equine kidney using quantitative real-time PCR (RT-qPCR), and then perform a comparison between the three species. Renal tissue samples were harvested post-mortem from 10 horses, 10 sheep, and five dogs. Primers were designed for each gene. For each sample total RNA was extracted, cDNA synthesised, and RT-qPCR was performed...
2016: PloS One
https://www.readbyqxmd.com/read/27572832/genomic-and-enzymatic-evidence-for-acetogenesis-among-multiple-lineages-of-the-archaeal-phylum-bathyarchaeota-widespread-in-marine-sediments
#20
Y He, M Li, V Perumal, X Feng, J Fang, J Xie, S M Sievert, F Wang
Members of the archaeal phylum Bathyarchaeota are widespread and abundant in the energy-deficient marine subsurface sediments. However, their life strategies have remained largely elusive. Here, we provide genetic evidence that some lineages of Bathyarchaeota are acetogens, being capable of homoacetogenesis, a metabolism so far restricted to the domain Bacteria. Metabolic reconstruction based on genomic bins assembled from the metagenome of deep-sea subsurface sediments shows that the metabolism of some lineages of Bathyarchaeota is similar to that of bona fide bacterial homoacetogens, by having pathways for acetogenesis and for the fermentative utilization of a variety of organic substrates...
2016: Nature Microbiology
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