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https://www.readbyqxmd.com/read/28293295/electrode-assisted-acetoin-production-in-a-metabolically-engineered-escherichia-coli-strain
#1
Andreas H Förster, Sebastian Beblawy, Frederik Golitsch, Johannes Gescher
BACKGROUND: This paper describes the metabolic engineering of Escherichia coli for the anaerobic fermentation of glucose to acetoin. Acetoin has well-established applications in industrial food production and was suggested to be a platform chemical for a bio-based economy. However, the biotechnological production is often hampered by the simultaneous formation of several end products in the absence of an electron acceptor. Moreover, typical production strains are often potentially pathogenic...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28131378/multiplex-pcr-based-identification-of-streptococcus-canis-streptococcus-zooepidemicus-and-streptococcus-dysgalactiae-subspecies-from-dogs
#2
M Moriconi, E Acke, D Petrelli, S Preziuso
Streptococcus canis (S. canis), Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) and Streptococcus dysgalactiae subspecies (S. dysgalactiae subspecies) are β-haemolytic Gram positive bacteria infecting animals and humans. S. canis and S. zooepidemicus are considered as two of the major zoonotic species of Streptococcus, while more research is needed on S. dysgalactiae subspecies bacteria. In this work, a multiplex-PCR protocol was tested on strains and clinical samples to detect S. canis, S. dysgalactiae subspecies and S...
February 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/28130304/ccpa-and-cody-coordinate-acetate-metabolism-in-streptococcus-mutans
#3
Jeong Nam Kim, Robert A Burne
In the dental caries pathogen Streptococcus mutans, phosphotransacetylase (Pta) and acetate kinase (Ack) convert pyruvate into acetate with the concomitant generation of ATP. The genes for this pathway are tightly regulated by multiple environmental and intracellular inputs, but the basis for differential expression of the genes for Pta and Ack in S. mutans had not been investigated. Here, we show that inactivation in S. mutans of ccpA or codY reduced the activity of the ackA promoter, whereas a ccpA mutant displayed elevated pta promoter activity...
April 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28113691/estimation-and-lqg-control-over-unreliable-network-with-acknowledgment-randomly-lost
#4
Hong Lin, Hongye Su, Peng Shi, Renquan Lu, Zheng-Guang Wu
In this paper, we study the state estimation and optimal control [i.e., linear quadratic Gaussian (LQG) control] problems for networked control systems in which control inputs, observations, and packet acknowledgments (ACKs) are randomly lost. The packet ACK is a signal that is transmitted from the actuator to notice the estimator the occurence of control packet loss. For such systems, we obtain the optimal estimator, which is consisted of exponentially increasing terms. For the solvability of the LQG problem, we come to a conclusion that in general even the optimal LQG control exists, it is impossible and unnecessary to be obtained as its calculation is not only technically difficult but also computationally prohibitive...
September 13, 2016: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28088294/suitability-of-small-diagnostic-peripheral-blood-samples-for-cell-therapy-studies
#5
Coralea Stephanou, Panayiota Papasavva, Myria Zachariou, Petros Patsali, Marilena Epitropou, Petros Ladas, Ruba Al-Abdulla, Soteroulla Christou, Michael N Antoniou, Carsten W Lederer, Marina Kleanthous
BACKGROUND AIMS: Primary hematopoietic stem and progenitor cells (HSPCs) are key components of cell-based therapies for blood disorders and are thus the authentic substrate for related research. We propose that ubiquitous small-volume diagnostic samples represent a readily available and as yet untapped resource of primary patient-derived cells for cell- and gene-therapy studies. METHODS: In the present study we compare isolation and storage methods for HSPCs from normal and thalassemic small-volume blood samples, considering genotype, density-gradient versus lysis-based cell isolation and cryostorage media with different serum contents...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/28075347/sdtcp-towards-datacenter-tcp-congestion-control-with-sdn-for-iot-applications
#6
Yifei Lu, Zhen Ling, Shuhong Zhu, Ling Tang
The Internet of Things (IoT) has gained popularity in recent years. Today's IoT applications are now increasingly deployed in cloud platforms to perform Big Data analytics. In cloud data center networks (DCN), TCP incast usually happens when multiple senders simultaneously communicate with a single receiver. However, when TCP incast happens, DCN may suffer from both throughput collapse for TCP burst flows and temporary starvation for TCP background flows. In this paper, we propose a software defined network (SDN)-based TCP congestion control mechanism, referred to as SDTCP, to leverage the features, e...
January 8, 2017: Sensors
https://www.readbyqxmd.com/read/28036001/tple-a-reliable-data-delivery-scheme-for-on-road-wsn-traffic-monitoring
#7
Rui Wang, Fei Chang, Suli Ren
In an on-road environment, motor-engines severely disturb the wireless link of a sensor node, leading to high package loss rate, high delivery delay, and poor radio communication quality. The existing data delivery mechanisms, such as the ACK-based retransmission mechanism and window-based link quality estimation mechanism, could not handle these challenges well. To solve this challenge, we propose a Target-Prediction-based Link quality Estimation scheme (TPLE) to realize high quality data delivery in an on-road environment...
December 27, 2016: Sensors
https://www.readbyqxmd.com/read/27860343/isolation-of-campylobacter-spp-from-client-owned-dogs-and-cats-and-retail-raw-meat-pet-food-in-the-manawatu-new-zealand
#8
K Bojanić, A C Midwinter, J C Marshall, L E Rogers, P J Biggs, E Acke
Campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. The disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. The prevalence of Campylobacter spp. in dogs and cats varies widely, and data on New Zealand pets are limited. This study aimed to investigate the prevalence of Campylobacter spp. in dogs, cats and retail raw meat pet food products in New Zealand and to characterize Campylobacter jejuni isolates using multilocus sequence typing (MLST)...
November 12, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/27738720/efficient-multi-enzyme-catalyzed-cdp-choline-production-driven-by-an-atp-donor-module
#9
Yingmiao Liu, Junzhi Wang, Chongmao Xu, Yong Chen, Junjie Yang, Dong Liu, Huanqing Niu, Yu Jiang, Sheng Yang, Hanjie Ying
Cytidine diphosphate choline (CDP-choline) has been applied for treating acute craniocerebral injury and allowing recovery of consciousness after brain surgery. In this study, an acetate kinase (ACK)/acetyl phosphate system was used to supply ATP and combined with Escherichia coli-overexpressed CMP kinase (CMK), NDP kinase (NDK), choline phosphate cytidylyltransferase (CCT), and choline kinase (CKI) to produce CDP-choline from CMP and choline chloride. Within 1 h, 49 mM CDP-choline was produced, for a molar yield of 89...
February 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27669243/gateway-assisted-retransmission-for-lightweight-and-reliable-iot-communications
#10
Hui-Ling Chang, Cheng-Gang Wang, Mong-Ting Wu, Meng-Hsun Tsai, Chia-Ying Lin
Message Queuing Telemetry Transport for Sensor Networks (MQTT-SN) and Constrained Application Protocol (CoAP) are two protocols supporting publish/subscribe models for IoT devices to publish messages to interested subscribers. Retransmission mechanisms are introduced to compensate for the lack of data reliability. If the device does not receive the acknowledgement (ACK) before retransmission timeout (RTO) expires, the device will retransmit data. Setting an appropriate RTO is important because the delay may be large or retransmission may be too frequent when the RTO is inappropriate...
September 22, 2016: Sensors
https://www.readbyqxmd.com/read/27632366/evaluation-and-comparison-of-vitamin-d-responsive-gene-expression-in-ovine-canine-and-equine-kidney
#11
Sara Azarpeykan, Keren E Dittmer, Jonathan C Marshall, Kalyani C Perera, Erica K Gee, Els Acke, Keith G Thompson
The aim of this study was to determine the relative abundance and relationship of vitamin D responsive and calcium transporting transcripts (TRPV5, TRPV6, calD9k, calD28k, PMCA, NCX1, CYP27B1, CYP24A1, and VDR) in ovine, canine and, equine kidney using quantitative real-time PCR (RT-qPCR), and then perform a comparison between the three species. Renal tissue samples were harvested post-mortem from 10 horses, 10 sheep, and five dogs. Primers were designed for each gene. For each sample total RNA was extracted, cDNA synthesised, and RT-qPCR was performed...
2016: PloS One
https://www.readbyqxmd.com/read/27572832/genomic-and-enzymatic-evidence-for-acetogenesis-among-multiple-lineages-of-the-archaeal-phylum-bathyarchaeota-widespread-in-marine-sediments
#12
Y He, M Li, V Perumal, X Feng, J Fang, J Xie, S M Sievert, F Wang
Members of the archaeal phylum Bathyarchaeota are widespread and abundant in the energy-deficient marine subsurface sediments. However, their life strategies have remained largely elusive. Here, we provide genetic evidence that some lineages of Bathyarchaeota are acetogens, being capable of homoacetogenesis, a metabolism so far restricted to the domain Bacteria. Metabolic reconstruction based on genomic bins assembled from the metagenome of deep-sea subsurface sediments shows that the metabolism of some lineages of Bathyarchaeota is similar to that of bona fide bacterial homoacetogens, by having pathways for acetogenesis and for the fermentative utilization of a variety of organic substrates...
2016: Nature Microbiology
https://www.readbyqxmd.com/read/27553086/ammonium-chloride-potassium-lysing-buffer-treatment-of-fully-differentiated-cells-increases-cell-purity-and-resulting-neotissue-functional-properties
#13
Wendy E Brown, Jerry C Hu, Kyriacos A Athanasiou
Juvenile and fetal, primary, fully differentiated cells are widely considered to be ideal cell types for tissue engineering applications. However, their use in tissue engineering may be hindered through contamination by undesirable cell types. These include blood-associated cells as well as unwanted resident cell types found both in healthy and pathologic donor tissues. Ammonium-chloride-potassium (ACK) lysing buffer is used to lyse red blood cells (RBCs) during the isolation of stem cell populations, but has not been explored for the purification of fully differentiated cells...
September 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27462444/ack-promotes-tissue-growth-via-phosphorylation-and-suppression-of-the-hippo-pathway-component-expanded
#14
Lianxin Hu, Jiajun Xu, Meng-Xin Yin, Liguo Zhang, Yi Lu, Wenqing Wu, Zhaoyu Xue, Margaret S Ho, Guanjun Gao, Yun Zhao, Lei Zhang
Non-receptor tyrosine kinase activated cdc42 kinase was reported to participate in several types of cancers in mammals. It is also believed to have an anti-apoptotic function in Drosophila. Here, we report the identification of Drosophila activated cdc42 kinase as a growth promoter and a novel Hippo signaling pathway regulator. We find that activated cdc42 kinase promotes tissue growth through modulating Yorkie activity. Furthermore, we demonstrate that activated cdc42 kinase interacts with Expanded and induces tyrosine phosphorylation of Expanded on multiple sites...
2016: Cell Discovery
https://www.readbyqxmd.com/read/27458439/industrial-acetogenic-biocatalysts-a-comparative-metabolic-and-genomic-analysis
#15
Frank R Bengelsdorf, Anja Poehlein, Sonja Linder, Catarina Erz, Tim Hummel, Sabrina Hoffmeister, Rolf Daniel, Peter Dürre
Synthesis gas (syngas) fermentation by anaerobic acetogenic bacteria employing the Wood-Ljungdahl pathway is a bioprocess for production of biofuels and biocommodities. The major fermentation products of the most relevant biocatalytic strains (Clostridium ljungdahlii, C. autoethanogenum, C. ragsdalei, and C. coskatii) are acetic acid and ethanol. A comparative metabolic and genomic analysis using the mentioned biocatalysts might offer targets for metabolic engineering and thus improve the production of compounds apart from ethanol...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27438061/the-role-of-reactive-oxygen-species-in-antibiotic-induced-cell-death-in-burkholderia-cepacia-complex-bacteria
#16
Heleen Van Acker, Jan Gielis, Marloes Acke, Freya Cools, Paul Cos, Tom Coenye
It was recently proposed that bactericidal antibiotics, besides through specific drug-target interactions, kill bacteria by a common mechanism involving the production of reactive oxygen species (ROS). However, this mechanism involving the production of hydroxyl radicals has become the subject of a lot of debate. Since the contribution of ROS to antibiotic mediated killing most likely depends on the conditions, differences in experimental procedures are expected to be at the basis of the conflicting results...
2016: PloS One
https://www.readbyqxmd.com/read/27431058/phospho-transfer-networks-and-atp-homeostasis-in-response-to-an-ineffective-electron-transport-chain-in-pseudomonas-fluorescens
#17
V P Appanna, A A Alhasawi, C Auger, S C Thomas, V D Appanna
Although oxidative stress is known to impede the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, the nutritionally-versatile microbe, Pseudomonas fluorescens has been shown to proliferate in the presence of hydrogen peroxide (H2O2) and nitrosative stress. In this study we demonstrate the phospho-transfer system that enables this organism to generate ATP was similar irrespective of the carbon source utilized. Despite the diminished activities of enzymes involved in the TCA cycle and in the electron transport chain (ETC), the ATP levels did not appear to be significantly affected in the stressed cells...
September 15, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27380686/we-need-abuse-training-for-all
#18
Bekki Lloyd
I support mandatory domestic abuse training for all healthcare workers (news, June 22). I attended an emergency department aft er my ex-partner had att acked me. No one asked if I was okay - it was more a case of clean me up, check for injuries and send me on my way. Clearly, the 6Cs were not implemented during my visit.
July 6, 2016: Nursing Standard
https://www.readbyqxmd.com/read/27317896/variation-in-the-limit-of-detection-of-the-prospect-campylobacter-microplate-enzyme-immunoassay-in-stools-spiked-with-emerging-campylobacter-species
#19
Krunoslav Bojanić, Anne Camilla Midwinter, Jonathan Craig Marshall, Lynn Elizabeth Rogers, Patrick Jon Biggs, Els Acke
Campylobacter enteritis in humans is primarily associated with C. jejuni/coli infection. The impact of other Campylobacter spp. is likely to be underestimated due to the bias of culture methods towards Campylobacter jejuni/coli diagnosis. Stool antigen tests are becoming increasingly popular and appear generally less species-specific. A review of independent studies of the ProSpecT® Campylobacter Microplate enzyme immunoassay (EIA) developed for C. jejuni/coli showed comparable diagnostic results to culture methods but the examination of non-jejuni/coli Campylobacter spp...
August 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27221631/tcdd-modulation-of-gut-microbiome-correlated-with-liver-and-immune-toxicity-in-streptozotocin-stz-induced-hyperglycemic-mice
#20
Daniel E Lefever, Joella Xu, Yingjia Chen, Guannan Huang, Nagy Tamas, Tai L Guo
An increasing body of evidence has shown the important role of the gut microbiome in mediating toxicity following environmental contaminant exposure. The goal of this study was to determine if the adverse metabolic effects of chronic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure would be sufficient to exacerbate hyperglycemia, and to further determine if these outcomes were attributable to the gut microbiota alteration. Adult male CD-1 mice were exposed to TCDD (6μg/kg body weight biweekly) by gavage and injected (i...
August 1, 2016: Toxicology and Applied Pharmacology
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