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https://www.readbyqxmd.com/read/28230503/super-resolution-in-light-microscopy
#1
Nuno Moreno, Nuno Pimpão Martins, Gabriel Martins
No abstract text is available yet for this article.
January 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28230156/drebrin-mediated-microtubule-actomyosin-coupling-steers-cerebellar-granule-neuron-nucleokinesis-and-migration-pathway-selection
#2
Niraj Trivedi, Daniel R Stabley, Blake Cain, Danielle Howell, Christophe Laumonnerie, Joseph S Ramahi, Jamshid Temirov, Ryan A Kerekes, Phillip R Gordon-Weeks, David J Solecki
Neuronal migration from a germinal zone to a final laminar position is essential for the morphogenesis of neuronal circuits. While it is hypothesized that microtubule-actomyosin crosstalk is required for a neuron's 'two-stroke' nucleokinesis cycle, the molecular mechanisms controlling such crosstalk are not defined. By using the drebrin microtubule-actin crosslinking protein as an entry point into the cerebellar granule neuron system in combination with super-resolution microscopy, we investigate how these cytoskeletal systems interface during migration...
February 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28226534/example-based-super-resolution-for-enhancing-spatial-resolution-of-medical-images
#3
Dai-Viet Tran, Sebastien Li-Thiao-Te, Marie Luong, Thuong Le-Tien, Francoise Dibos, Jean-Marie Rocchisani, Dai-Viet Tran, Sebastien Li-Thiao-Te, Marie Luong, Thuong Le-Tien, Francoise Dibos, Jean-Marie Rocchisani, Sebastien Li-Thiao-Te, Francoise Dibos, Jean-Marie Rocchisani, Marie Luong, Dai-Viet Tran, Thuong Le-Tien
This paper proposes an effective exampled-based super-resolution (SR) method to improve the spatial resolution of medical image heavily corrupted by noise. Based on the sparsity of patches, the reconstruction of a high-resolution (HR) patch from each low-resolution (LR) input patch can be performed with the help of a database, by solving a non-negative sparse optimization problem. The challenge is to effectively solve this problem in case of a large size database. To cope with this issue, we propose a metric to measure the similarity between image patches based on the Earth Mover's Distance (EMD) in order to select only the most similar candidates that will be used in the optimization problem...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225761/amplified-stimulated-emission-in-upconversion-nanoparticles-for-super-resolution-nanoscopy
#4
Yujia Liu, Yiqing Lu, Xusan Yang, Xianlin Zheng, Shihui Wen, Fan Wang, Xavier Vidal, Jiangbo Zhao, Deming Liu, Zhiguang Zhou, Chenshuo Ma, Jiajia Zhou, James A Piper, Peng Xi, Dayong Jin
Lanthanide-doped glasses and crystals are attractive for laser applications because the metastable energy levels of the trivalent lanthanide ions facilitate the establishment of population inversion and amplified stimulated emission at relatively low pump power. At the nanometre scale, lanthanide-doped upconversion nanoparticles (UCNPs) can now be made with precisely controlled phase, dimension and doping level. When excited in the near-infrared, these UCNPs emit stable, bright visible luminescence at a variety of selectable wavelengths, with single-nanoparticle sensitivity, which makes them suitable for advanced luminescence microscopy applications...
February 22, 2017: Nature
https://www.readbyqxmd.com/read/28223507/imaging-mrna-and-protein-interactions-within-neurons
#5
Carolina Eliscovich, Shailesh M Shenoy, Robert H Singer
RNA-protein interactions are essential for proper gene expression regulation, particularly in neurons with unique spatial constraints. Currently, these interactions are defined biochemically, but a method is needed to evaluate them quantitatively within morphological context. Colocalization of two-color labels using wide-field microscopy is a method to infer these interactions. However, because of chromatic aberrations in the objective lens, this approach lacks the resolution to determine whether two molecules are physically in contact or simply nearby by chance...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28219682/epilepsy-and-synaptic-proteins
#6
REVIEW
Yuko Fukata, Masaki Fukata
Throughout history, epilepsy affects about 1-2% of the population worldwide. Epilepsy can be caused by traumatic brain injury, exposure to certain toxins and drugs, and mutations of genes that often encode synaptic proteins. In addition to conventional linkage and association studies, the recent trio exome sequencing in epilepsy and proteomic analysis in autoimmune synaptopathies have accelerated identification of novel epilepsy-related proteins, most of which play critical roles in synaptic transmission. Furthermore, super-resolution microscopy analysis has revealed subsynaptic nanoscale distribution of presynaptic and postsynaptic proteins and suggests a precise trans-synaptic alignment of neurotransmitter release to receptors...
February 17, 2017: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/28217792/super-resolution-imaging-of-self-assembly-of-amphiphilic-photoswitchable-macrocycles
#7
Qiong-Xin Hua, Bo Xin, Zu-Jing Xiong, Wen-Liang Gong, Chong Li, Zhen-Li Huang, Ming-Qiang Zhu
Self-assembly of an amphiphilic photoswitchable fluorescent macrocycle methoxy-tetraethylene glycol-substituted hexaarylbiimidazole-borondipyrromethene can be observed directly under a super-resolution fluorescence microscope, with the nanoscale resolution beyond the optical diffraction limitation.
February 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28211677/super-resolution-microscopy-shedding-light-on-the-cellular-plasma-membrane
#8
Matthew B Stone, Sarah A Shelby, Sarah L Veatch
Lipids and the membranes they form are fundamental building blocks of cellular life, and their geometry and chemical properties distinguish membranes from other cellular environments. Collective processes occurring within membranes strongly impact cellular behavior and biochemistry, and understanding these processes presents unique challenges due to the often complex and myriad interactions between membrane components. Super-resolution microscopy offers a significant gain in resolution over traditional optical microscopy, enabling the localization of individual molecules even in densely labeled samples and in cellular and tissue environments...
February 17, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28211479/decoupling-absorption-and-emission-processes-in-super-resolution-localization-of-emitters-in-a-plasmonic-hotspot
#9
David L Mack, Emiliano Cortés, Vincenzo Giannini, Peter Török, Tyler Roschuk, Stefan A Maier
The absorption process of an emitter close to a plasmonic antenna is enhanced due to strong local electromagnetic (EM) fields. The emission, if resonant with the plasmonic system, re-radiates to the far-field by coupling with the antenna via plasmonic states, whose presence increases the local density of states. Far-field collection of the emission of single molecules close to plasmonic antennas, therefore, provides mixed information of both the local EM field strength and the local density of states. Moreover, super-resolution localizations from these emission-coupled events do not report the real position of the molecules...
February 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28208837/projections-onto-convex-sets-super-resolution-reconstruction-based-on-point-spread-function-estimation-of-low-resolution-remote-sensing-images
#10
Chong Fan, Chaoyun Wu, Grand Li, Jun Ma
To solve the problem on inaccuracy when estimating the point spread function (PSF) of the ideal original image in traditional projection onto convex set (POCS) super-resolution (SR) reconstruction, this paper presents an improved POCS SR algorithm based on PSF estimation of low-resolution (LR) remote sensing images. The proposed algorithm can improve the spatial resolution of the image and benefit agricultural crop visual interpolation. The PSF of the highresolution (HR) image is unknown in reality. Therefore, analysis of the relationship between the PSF of the HR image and the PSF of the LR image is important to estimate the PSF of the HR image by using multiple LR images...
February 13, 2017: Sensors
https://www.readbyqxmd.com/read/28206746/direct-formation-of-large-area-2d-nanosheets-from-fluorescent-semiconducting-homopolymer-with-orthorhombic-crystalline-orientation
#11
Sanghee Yang, Suyong Shin, Inho Choi, Jaeho Lee, Tae-Lim Choi
Semiconducting polymers have been widely investigated due to their intriguing optoelectronic properties and their high crystallinity that provides a strong driving force for self-assembly. Although there are various reports of successful self-assembly of nanostructures using semiconducting polymers, direct in situ self-assembly of these polymers into two-dimensional (2D) nanostructures has proven difficult, despite their importance for optoelectronics applications. Here, we report the synthesis of a simple conjugated homopolymer by living cyclopolymerization of a 1,6-heptadiyne (having a fluorene moiety) and its efficient in situ formation of large-area 2D fluorescent semiconducting nanostructures...
February 16, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28202688/super-resolution-mapping-of-scaffold-nucleoporins-in-the-nuclear-pore-complex
#12
Jiong Ma, Joseph M Kelich, Samuel L Junod, Weidong Yang
The nuclear pore complex (NPC), composed of ∼30 different nucleoporins (Nups), is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring-scaffold perforates the nuclear envelope and features a unique molecular machinery that regulates nucleocytoplasmic transport. However, the precise copy number and the spatial location of each Nup in the native NPC remain obscure due to the inherent difficulty of counting and localizing proteins inside the sub-micrometer supramolecular complex...
February 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28198852/influence-of-the-background-medium-on-imaging-performance-of-microsphere-assisted-super-resolution-microscopy
#13
Arash Darafsheh
We demonstrated the importance of the refractive index of the background medium containing the microsphere in microsphere-assisted super-resolution microscopy. We performed numerical simulation and showed that the photonic nanojet properties of a microlens can be optimized by adjusting the index of the background medium surrounding it. Our simulation results, in qualitative agreement with experimental observations, demonstrated that for the same refractive index contrast between the microsphere and its surrounding medium, a high-index microsphere embedded in a medium provides sharper focusing and better imaging quality compared with its low-index counterpart working in air...
February 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28197066/patch-based-super-resolution-of-mr-spectroscopic-images-application-to-multiple-sclerosis
#14
Saurabh Jain, Diana M Sima, Faezeh Sanaei Nezhad, Gilbert Hangel, Wolfgang Bogner, Stephen Williams, Sabine Van Huffel, Frederik Maes, Dirk Smeets
Purpose: Magnetic resonance spectroscopic imaging (MRSI) provides complementary information to conventional magnetic resonance imaging. Acquiring high resolution MRSI is time consuming and requires complex reconstruction techniques. Methods: In this paper, a patch-based super-resolution method is presented to increase the spatial resolution of metabolite maps computed from MRSI. The proposed method uses high resolution anatomical MR images (T1-weighted and Fluid-attenuated inversion recovery) to regularize the super-resolution process...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28196307/molecule-counts-in-localization-microscopy-with-organic-fluorophores
#15
Mike Heilemann, Franziska Fricke, Christos Karathanasis, Gerhard Hummer
Single-molecule localization microscopy (SMLM) can be used to count fluorescently labeled molecules even when they are not individually resolved. We demonstrate SMLM molecule counting for nucleic acids labeled with the organic fluorophore Alexa Fluor 647 and imaged under photoswitching conditions. From the observed distributions of the number of fluorophore blinking events, we extracted the number of fluorophores per spot using a statistical model. We validate the molecule counting method for single Alexa Fluor 647 fluorophores, and for trimers of Alexa Fluor 647 constructed on a DNA origami structure...
February 14, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28195833/a-generalized-framework-unifying-image-registration-and-respiratory-motion-models-and-incorporating-image-reconstruction-for-partial-image-data-or-full-images
#16
Jamie R McClelland, Marc Modat, Simon Arridge, Helen Grimes, Derek D'Souza, David Thomas, Dylan O'Connell, Daniel Low, Evangelia Kaza, David Collins, Martin Leach, David Hawkes
Surrogate-driven respiratory motion models relate the motion of the internal anatomy to easily acquired respiratory surrogate signals, such as the motion of the skin surface. They are usually built by first using image registration to determine the motion from a number of dynamic images, and then fitting a correspondence model relating the motion to the surrogate signals. In this paper we present a generalized framework that unifies the image registration and correspondence model fitting into a single optimization...
February 14, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28194645/expression-of-a-novel-ciliary-protein-iiig9-during-the-differentiation-and-maturation-of-ependymal-cells
#17
M Cifuentes, V Baeza, P M Arrabal, R Visser, J M Grondona, N Saldivia, F Martínez, F Nualart, K Salazar
IIIG9 is the regulatory subunit 32 of protein phosphatase 1 (PPP1R32), a key phosphatase in the regulation of ciliary movement. IIIG9 localization is restricted to cilia in the trachea, fallopian tube, and testicle, suggesting its involvement in the polarization of ciliary epithelium. In the adult brain, IIIG9 mRNA has only been detected in ciliated ependymal cells that cover the ventricular walls. In this work, we prepared a polyclonal antibody against rat IIIG9 and used this antibody to show for the first time the ciliary localization of this protein in adult ependymal cells...
February 13, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28192299/single-molecule-analysis-of-fluorescent-carbon-dots-towards-localization-based-super-resolution-microscopy
#18
Navneet C Verma, Syamantak Khan, Chayan K Nandi
The advancement of high-resolution bioimaging has always been dependent on the discovery of bright and easily available fluorescent probes. Fluorescent carbon nanodots, an interesting class of relatively new nanomaterials, have emerged as a versatile alternative due to their superior optical properties, non-toxicity, cell penetrability and easy routes to synthesis. Although a plethora of reports is available on bioimaging using carbon dots, single-molecule-based super-resolution imaging is rare in the literature...
October 11, 2016: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28186887/accelerated-singular-value-based-ultrasound-blood-flow-clutter-filtering-with-randomized-singular-value-decomposition-rsvd-and-randomized-spatial-downsampling
#19
Pengfei Song, Joshua Trzasko, Armando Manduca, Bo Qiang, Ramanathan Kadirvel, David Kallmes, Shigao Chen
Singular value decomposition (SVD)-based ultrasound blood flow clutter filters have recently demonstrated substantial improvement in clutter rejection for ultrafast plane wave microvessel imaging, and have become the commonly used clutter filtering method for many novel ultrafast imaging applications such as functional ultrasound and super-resolution imaging. At present, however, the computational burden of SVD remains as a major hurdle for practical implementation and clinical translation of this method. To address this challenge, in the study we present two blood flow clutter filtering methods based on randomized singular value decomposition (rSVD) and randomized spatial downsampling to accelerate SVD clutter filtering with minimal compromise to the clutter filter performance...
February 7, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28185951/evaluation-of-slice-dithered-enhanced-resolution-simultaneous-multislice-slider-sms-for-human-fmri
#20
An T Vu, Alex Beckett, Kawin Setsompop, David A Feinberg
High isotropic resolution fMRI is challenging primarily due to long repetition times (TR) and insufficient SNR, especially at lower field strengths. Recently, Simultaneous Multi-Slice (SMS) imaging with blipped-CAIPI has substantially reduced scan time and improved SNR efficiency of fMRI. Similarly, super-resolution techniques utilizing sub- voxel spatial shifts in the slice direction have increased both resolution and SNR efficiency. Here we demonstrate the synergistic combination of SLIce Dithered Enhanced Resolution (SLIDER) and SMS for high-resolution, high-SNR whole brain fMRI in comparison to standard resolution fMRI data as well as high-resolution data...
February 6, 2017: NeuroImage
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