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Aspergillus fumigatus transcriptome

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https://www.readbyqxmd.com/read/29753128/selenate-sensitivity-of-a-laea-mutant-is-restored-by-overexpression-of-the-bzip-protein-metr-in-aspergillus-fumigatus
#1
Sachin Jain, Relebohile Sekonyela, Ben Knox, Jonathan M Palmer, Anna Huttenlocher, Mehdi Kabbage, Nancy P Keller
LaeA is a conserved global regulator of secondary metabolism and development in filamentous fungi. Examination of Aspergillus fumigatus transcriptome data of laeA deletion mutants have been fruitful in identifying genes and molecules contributing to the laeA mutant phenotype. One of the genes significantly down regulated in A. fumigatus ΔlaeA is metR, encoding a bZIP DNA binding protein required for sulfur and methionine metabolism in fungi. LaeA and MetR deletion mutants exhibit several similarities including down regulation of sulfur assimilation and methionine metabolism genes and ability to grow on the toxic sulfur analog, sodium selenate...
May 9, 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29747589/additional-oxidative-stress-reroutes-the-global-response-of-aspergillus-fumigatus-to-iron-depletion
#2
Vivien Kurucz, Thomas Krüger, Károly Antal, Anna-Maria Dietl, Hubertus Haas, István Pócsi, Olaf Kniemeyer, Tamás Emri
BACKGROUND: Aspergillus fumigatus has to cope with a combination of several stress types while colonizing the human body. A functional interplay between these different stress responses can increase the chances of survival for this opportunistic human pathogen during the invasion of its host. In this study, we shed light on how the H2 O2 -induced oxidative stress response depends on the iron available to this filamentous fungus, using transcriptomic analysis, proteomic profiles, and growth assays...
May 10, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29706948/heavy-metal-induced-expression-of-pcaa-provides-cadmium-tolerance-to-aspergillus-fumigatus-and-supports-its-virulence-in-the-galleria-mellonella-model
#3
Fruzsina Bakti, Christoph Sasse, Thorsten Heinekamp, István Pócsi, Gerhard H Braus
Most of the metal transporters in Aspergillus fumigatus are yet uncharacterized. Their role in fungal metabolism and virulence remains unclear. This paper describes the novel PIB-type cation ATPase PcaA, which links metal homeostasis and heavy metal tolerance in the opportunistic human pathogen A. fumigatus . The protein possesses conserved ATPase motif and shares 51% amino acid sequence identity with the Saccharomyces cerevisiae cadmium exporter Pca1p. A pcaA deletion, an overexpression and a gfp-pcaA complementation strain of A...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29705402/gene-co-expression-analysis-identifies-gene-clusters-associated-with-isotropic-and-polarized-growth-in-aspergillus-fumigatus-conidia
#4
Tim J H Baltussen, Jordy P M Coolen, Jan Zoll, Paul E Verweij, Willem J G Melchers
Aspergillus fumigatus is a saprophytic fungus that extensively produces conidia. These microscopic asexually reproductive structures are small enough to reach the lungs. Germination of conidia followed by hyphal growth inside human lungs is a key step in the establishment of infection in immunocompromised patients. RNA-Seq was used to analyze the transcriptome of dormant and germinating A. fumigatus conidia. Construction of a gene co-expression network revealed four gene clusters (modules) correlated with a growth phase (dormant, isotropic growth, polarized growth)...
April 26, 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29614953/transcriptome-and-secretome-analysis-of-aspergillus-fumigatus-in-the-presence-of-sugarcane-bagasse
#5
Paula Fagundes de Gouvêa, Aline Vianna Bernardi, Luis Eduardo Gerolamo, Emerson de Souza Santos, Diego Mauricio Riaño-Pachón, Sergio Akira Uyemura, Taisa Magnani Dinamarco
BACKGROUND: Sugarcane bagasse has been proposed as a lignocellulosic residue for second-generation ethanol (2G) produced by breaking down biomass into fermentable sugars. The enzymatic cocktails for biomass degradation are mostly produced by fungi, but low cost and high efficiency can consolidate 2G technologies. A. fumigatus plays an important role in plant biomass degradation capabilities and recycling. To gain more insight into the divergence in gene expression during steam-exploded bagasse (SEB) breakdown, this study profiled the transcriptome of A...
April 3, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29564395/an-laea-and-brla-dependent-cellular-network-governs-tissue-specific-secondary-metabolism-in-the-human-pathogen-aspergillus-fumigatus
#6
Abigail L Lind, Fang Yun Lim, Alexandra A Soukup, Nancy P Keller, Antonis Rokas
Biosynthesis of many ecologically important secondary metabolites (SMs) in filamentous fungi is controlled by several global transcriptional regulators, like the chromatin modifier LaeA, and tied to both development and vegetative growth. In Aspergillus molds, asexual development is regulated by the BrlA > AbaA > WetA transcriptional cascade. To elucidate BrlA pathway involvement in SM regulation, we examined the transcriptional and metabolic profiles of Δ brlA , Δ abaA , and Δ wetA mutant and wild-type strains of the human pathogen Aspergillus fumigatus ...
March 2018: MSphere
https://www.readbyqxmd.com/read/29500637/rna-seq-profile-reveals-th-1-and-th-17-type-of-immune-responses-in-mice-infected-systemically-with-aspergillus-fumigatus
#7
Jata Shankar, Gustavo C Cerqueira, Jennifer R Wortman, Karl V Clemons, David A Stevens
With the increasing numbers of immunocompromised hosts, Aspergillus fumigatus emerges as a lethal opportunistic fungal pathogen. Understanding innate and acquired immunity responses of the host is important for a better therapeutic strategy to deal with aspergillosis patients. To determine the transcriptome in the kidneys in aspergillosis, we employed RNA-Seq to obtain single 76-base reads of whole-genome transcripts of murine kidneys on a temporal basis (days 0; uninfected, 1, 2, 3 and 8) during invasive aspergillosis...
March 2, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29402803/identification-of-immunoglobulin-e-binding-proteins-of-the-xerophilic-fungus-aspergillus-penicillioides-crude-mycelial-mat-extract-and-serological-reactivity-assessment-in-subjects-with-different-allergen-reactivity-profiles
#8
Joenice González De León, Ricardo González Méndez, Carmen L Cadilla, Félix E Rivera-Mariani, Benjamín Bolaños-Rosero
BACKGROUND: Aspergillus penicillioides is a very common indoor xerophilic fungus and potential causative agent of respiratory conditions. Although people are constantly exposed to A. penicillioides, no proteins with allergenic potential have been described. Therefore, we aim to confirm allergic sensitization to A. penicillioides through reactivity in serological assays and detect immunoglobulin E (IgE)-binding proteins. METHODS: In an indirect ELISA, we compared the serological reactivity to A...
2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29345613/comparative-transcriptomics-of-aspergillus-fumigatus-strains-upon-exposure-to-human-airway-epithelial-cells
#9
Tonya N Watkins, Hong Liu, Matthew Chung, Tracy H Hazen, Julie C Dunning Hotopp, Scott G Filler, Vincent M Bruno
Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mould that can cause severe allergic responses in atopic individuals as well as life-threatening infections in immunocompromised patients. A critical step in the establishment of infection is the invasion of airway epithelial cells by the inhaled fungi. Understanding how A. fumigatus senses and responds to airway cells is important to understand the pathogenesis of invasive pulmonary aspergillosis. Here, we analysed the transcriptomes of two commonly used clinical isolates, Af293 and CEA10, during infection of the A549 type II pneumocyte cell line in vitro...
January 18, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29209049/repurposing-an-inhibitor-of-ribosomal-biogenesis-with-broad-anti-fungal-activity
#10
Nuo Sun, Dongmei Li, Yuhan Zhang, Kyle Killeen, William Groutas, Richard Calderone
The lack of new antifungal compounds with unique mechanisms of action is a concern for therapeutic management of patients. To identify inhibitors against human pathogenic fungi, we screened ~3000 compounds provided by the Developmental Therapeutics Program of NIH/NCI against a panel of pathogenic fungi including Candida species, Aspergillus fumigatus, and Cryptococcus neoformans. NSC319726 (a thiosemicarbazone) had broad antifungal activity in the range of 0.1-2.0 µg/ml and was also inhibitory to fluconazole-resistant isolates of Candida species...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29202712/global-gene-expression-reveals-stress-responsive-genes-in-aspergillus-fumigatus-mycelia
#11
Hiroki Takahashi, Yoko Kusuya, Daisuke Hagiwara, Azusa Takahashi-Nakaguchi, Kanae Sakai, Tohru Gonoi
BACKGROUND: Aspergillus fumigatus is a human fungal pathogen that causes aspergillosis in immunocompromised hosts. A. fumigatus is believed to be exposed to diverse environmental stresses in the host cells. The adaptation mechanisms are critical for infections in human bodies. Transcriptional networks in response to diverse environmental challenges remain to be elucidated. To gain insights into the adaptation to environmental stresses in A. fumigatus mycelia, we conducted time series transcriptome analyses...
December 4, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29113011/aspergillus-fumigatus-adhesion-factors-in-dormant-conidia-revealed-through-comparative-phenotypic-and-transcriptomic-analyses
#12
Azusa Takahashi-Nakaguchi, Kanae Sakai, Hiroki Takahashi, Daisuke Hagiwara, Takahito Toyotome, Hiroji Chibana, Akira Watanabe, Takashi Yaguchi, Masashi Yamaguchi, Katsuhiko Kamei, Tohru Gonoi
Aspergillus fumigatus is an important fungal pathogen of humans. Inhaled conidia of A. fumigatus adhere to pulmonary epithelial cells, causing opportunistic infection. However, little is known about the molecular mechanism of the adherence of resting conidia. Fungal molecules adhesive to host cells are presumed to be displayed on the conidial surface during conidial formation as a result of changes in gene expression. Therefore, we exhaustively searched for adhesion molecules by comparing the phenotypes and the gene expression profiles of A...
March 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29109714/characteristics-of-a-regulator-of-g-protein-signaling-rgs-rgsc-in-aspergillus-fumigatus
#13
Young Kim, In-Beom Heo, Jae-Hyuk Yu, Kwang-Soo Shin
The regulator of G-protein signaling (RGS) proteins have a conserved RGS domain that facilitates the intrinsic GTPase activity of an activated Gα subunit of heterotrimeric G protein, thereby attenuating signal transduction. Among six predicted RGS proteins in the opportunistic human pathogenic fungus Aspergillus fumigatus , only three (FlbA, GprK, and Rax1) have been studied. The unexplored RgsC composed of the Phox-associated (PXA), RGS, Phox homology (PX), and Nexin_C superfamily domains is highly conserved in many ascomycete fungi, suggesting a crucial role of RgsC in fungal biology...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28922497/mutual-independence-of-alkaline-and-calcium-mediated-signalling-in-aspergillus-fumigatus-refutes-the-existence-of-a-conserved-druggable-signalling-nexus
#14
Omar Loss, Margherita Bertuzzi, Yu Yan, Natalie Fedorova, Bethany L McCann, Darius Armstrong-James, Eduardo A Espeso, Nick D Read, William C Nierman, Elaine M Bignell
Functional coupling of calcium- and alkaline responsive signalling occurs in multiple fungi to afford efficient cation homeostasis. Host microenvironments exert alkaline stress and potentially toxic concentrations of Ca2+ , such that highly conserved regulators of both calcium- (Crz) and pH- (PacC/Rim101) responsive signalling are crucial for fungal pathogenicity. Drugs targeting calcineurin are potent antifungal agents but also perturb human immunity thereby negating their use as anti-infectives, abrogation of alkaline signalling has, therefore, been postulated as an adjunctive antifungal strategy...
December 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28753224/aspergillus%C3%A2-fumigatus-protein-phosphatase-ppza-is-involved-in-iron-assimilation-secondary-metabolite-production-and-virulence
#15
Adriana Oliveira Manfiolli, Patrícia Alves de Castro, Thaila Fernanda Dos Reis, Stephen Dolan, Sean Doyle, Gary Jones, Diego M Riaño Pachón, Mevlüt Ulaş, Luke M Noble, Derek J Mattern, Axel A Brakhage, Vito Valiante, Rafael Silva-Rocha, Ozgur Bayram, Gustavo H Goldman
Metal restriction imposed by mammalian hosts during an infection is a common mechanism of defence to reduce or avoid the pathogen infection. Metals are essential for organism survival due to its involvement in several biological processes. Aspergillus fumigatus causes invasive aspergillosis, a disease that typically manifests in immunocompromised patients. A. fumigatus PpzA, the catalytic subunit of protein phosphatase Z (PPZ), has been recently identified as associated with iron assimilation. A. fumigatus has 2 high-affinity mechanisms of iron acquisition during infection: reductive iron assimilation and siderophore-mediated iron uptake...
July 28, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/27706915/genome-wide-transcriptome-analysis-of-aspergillus-fumigatus-exposed-to-osmotic-stress-reveals-regulators-of-osmotic-and-cell-wall-stresses-that-are-saka-hog1-and-mpkc-dependent
#16
Lilian Pereira Silva, Patrícia Alves de Castro, Thaila Fernanda Dos Reis, Mario Henrique Paziani, Márcia Regina Von Zeska Kress, Diego M Riaño-Pachón, Daisuke Hagiwara, Laure N A Ries, Neil Andrew Brown, Gustavo H Goldman
Invasive aspergillosis is predominantly caused by Aspergillus fumigatus, and adaptations to stresses experienced within the human host are a prerequisite for the survival and virulence strategies of the pathogen. The central signal transduction pathway operating during hyperosmotic stress is the high osmolarity glycerol mitogen-activated protein kinase cascade. A. fumigatus MpkC and SakA, orthologues of the Saccharomyces cerevisiae Hog1p, constitute the primary regulator of the hyperosmotic stress response...
April 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/27664992/transcript-levels-of-the-aspergillus-fumigatus-cdc42-module-polarisome-and-septin-genes-show-little-change-from-dormancy-to-polarity-establishment
#17
Ken Oda, Elaine Bignell, S Earl Kang, Michelle Momany
Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine the A. fumigatus transcriptome during early development, focusing on the isotropic to polar switch...
June 1, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27584150/characterization-of-gprk-encoding-a-putative-hybrid-g-protein-coupled-receptor-in-aspergillus-fumigatus
#18
Mun-Gu Jung, Sung Su Kim, Jae-Hyuk Yu, Kwang-Soo Shin
The G-protein-coupled receptor (GPCR) family represents the largest and most varied collection of membrane embedded proteins that are sensitized by ligand binding and interact with heterotrimeric G proteins. Despite their presumed critical roles in fungal biology, the functions of the GPCR family members in the opportunistic human pathogen Aspergillus fumigatus are largely unknown, as only two (GprC and GprD) of the 15 predicted GPCRs have been studied. Here, we characterize the gprK gene, which is predicted to encode a hybrid GPCR with both 7-transmembrane and regulator of G-protein signaling (RGS) domains...
2016: PloS One
https://www.readbyqxmd.com/read/27538790/the-aspergillus-fumigatus-scha-sch9-kinase-modulates-saka-hog1-map-kinase-activity-and-it-is-essential-for-virulence
#19
Patrícia Alves de Castro, Thaila Fernanda Dos Reis, Stephen K Dolan, Adriana Oliveira Manfiolli, Neil Andrew Brown, Gary W Jones, Sean Doyle, Diego M Riaño-Pachón, Fábio Márcio Squina, Camila Caldana, Ashutosh Singh, Maurizio Del Poeta, Daisuke Hagiwara, Rafael Silva-Rocha, Gustavo H Goldman
The serine-threonine kinase TOR, the Target of Rapamycin, is an important regulator of nutrient, energy and stress signaling in eukaryotes. Sch9, a Ser/Thr kinase of AGC family (the cAMP-dependent PKA, cGMP- dependent protein kinase G and phospholipid-dependent protein kinase C family), is a substrate of TOR. Here, we characterized the fungal opportunistic pathogen Aspergillus fumigatus Sch9 homologue (SchA). The schA null mutant was sensitive to rapamycin, high concentrations of calcium, hyperosmotic stress and SchA was involved in iron metabolism...
November 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27185182/comparative-transcriptome-analysis-revealing-dormant-conidia-and-germination-associated-genes-in-aspergillus-species-an-essential-role-for-atfa-in-conidial-dormancy
#20
Daisuke Hagiwara, Hiroki Takahashi, Yoko Kusuya, Susumu Kawamoto, Katsuhiko Kamei, Tohru Gonoi
BACKGROUND: Fungal conidia are usually dormant unless the extracellular conditions are right for germination. Despite the importance of dormancy, little is known about the molecular mechanism underlying entry to, maintenance of, and exit from dormancy. To gain comprehensive and inter-species insights, transcriptome analyses were conducted across Aspergillus fumigatus, Aspergillus niger, and Aspergillus oryzae. RESULTS: We found transcripts of 687, 694, and 812 genes were enriched in the resting conidia compared with hyphae in A...
May 17, 2016: BMC Genomics
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