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Glutaraldehyde 2%

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https://www.readbyqxmd.com/read/28208017/tailoring-cellular-uptake-and-fluorescence-of-poly-2-oxazoline-based-nanogels
#1
Ulrich S Schubert, Matthias Hartlieb, Tanja Bus, Joachim Kübel, David Pretzel, Stephanie Hoeppener, Meike N Leiske, Kristian Kempe, Benjamin Dietzek
Controlling the size and charge of nanometer sized objects is of upmost importance for their interactions with cells. We herein present the synthesis of poly(2-oxazoline) based nanogels comprising a hydrophilic shell and an amine containing core compartment. Amine groups were cross-linked using glutaraldehyde resulting in imine based nanogels. As a drug model, amino fluorescein was covalently immobilized within the core, quenching excessive aldehyde functions. By varying the amount of cross-linker, the zeta potential and, hence, the cellular uptake could be adjusted...
February 16, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28199953/evaluation-of-single-and-stack-membraneless-enzymatic-fuel-cells-based-on-ethanol-in-simulated-body-fluids
#2
J Galindo-de-la-Rosa, N Arjona, A Moreno-Zuria, E Ortiz-Ortega, M Guerra-Balcázar, J Ledesma-García, L G Arriaga
The purpose of this work is to evaluate single and double-cell membraneless microfluidic fuel cells (MMFCs) that operate in the presence of simulated body fluids SBF, human serum and blood enriched with ethanol as fuels. The study was performed using the alcohol dehydrogenase enzyme immobilised by covalent binding through an array composed of carbon Toray paper as support and a layer of poly(methylene blue)/tetrabutylammonium bromide/Nafion and glutaraldehyde (3D bioanode electrode). The single MMFC was tested in a hybrid microfluidic fuel cell using Pt/C as the cathode...
February 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28198974/effect-of-two-desensitizing-agents-on-dentin-permeability-in-vitro
#3
Hiroshi Ishihata, Masafumi Kanehira, Werner J Finger, Hidekazu Takahashi, Makoto Tomita, Keiichi Sasaki
Objective: The aim of this in vitro study was to investigate the effect of two desensitizing agents and water on hydraulic conductance in human dentin. Material and Methods: GLUMA Desensitizer PowerGel (GLU) contains glutaraldehyde (GA) and 2-hydroxyethyl methacrylate (HEMA), and Teethmate Desensitizer (TD) is a powder comprising tetracalcium phosphate (TTCP) and dicalcium phosphate anhydrous (DCPA) that is mixed with water. Deionized water was used as a negative control (CTR)...
January 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28196414/polyelectrolyte-fiber-assembly-of-plant-derived-spider-silk-like-proteins
#4
Congyue Annie Peng, Julia A Russo, Todd A Lyda, William Richard Marcotte
Spider dragline silk is a proteinaceous material that combines superior toughness and biocompatibility, a promising bio-material. The distinct protein structure and the fiber formation process contribute to the superior toughness of dragline silk. Previously, we have produced recombinant spider silk-like proteins in transgenic tobacco that are readily purified from plant extracts. The plant-derived spidroin-like proteins consisted of native major ampullate spidroin 1 or spidroin 2 N- and C-termini flanking 8, 16, or 32 copies of their respective consensus block repeats (mini-spidroins)...
February 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28188350/amperometric-l-lysine-enzyme-electrodes-based-on-carbon-nanotube-redox-polymer-and-graphene-carbon-nanotube-redox-polymer-composites
#5
Ceren Kaçar, Pınar Esra Erden, Esma Kılıç
Highly sensitive L-lysine enzyme electrodes were constructed by using poly(vinylferrocene)-multiwalled carbon nanotubes-gelatine (PVF/MWCNTs-GEL) and poly(vinylferrocene)-multiwalled carbon nanotubes-gelatine-graphene (PVF/MWCNTs-GEL/GR) composites as sensing interfaces and their performances were evaluated. Lysine oxidase (LO) was immobilized onto the composite modified glassy carbon electrodes (GCE) by crosslinking using glutaraldehyde and bovine serum albumin. Effects of pH value, enzyme loading, applied potential, electrode composition, and interfering substances on the amperometric response of the enzyme electrodes were discussed...
February 10, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28187299/development-of-biohybrid-immuno-selective-membranes-for-target-antigen-recognition
#6
Francesca Militano, Teresa Poerio, Rosalinda Mazzei, Simona Salerno, Loredana De Bartolo, Lidietta Giorno
Membranes are gaining increasing interest in solid-phase analytical assay and biosensors applications, in particular as functional surface for bioreceptors immobilization and stabilization as well as for the concentration of target molecules in microsystems. In this work, regenerated cellulose immuno-affinity membranes were developed and they were used for the selective capture of interleukin-6 (IL-6) as targeted antigen. Protein G was covalently linked on the membrane surface and it was successfully used for the oriented site-specific antibody immobilization...
February 3, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28185908/purification-and-biophysical-characterization-of-the-aimp2-dx2-protein
#7
Roshan Jha, Hye Young Cho, Ameeq Ul Mushtaq, Kiho Lee, Dae Gyu Kim, Sunghoon Kim, Young Ho Jeon
Besides their primary role in protein synthesis, aminoacyl-tRNA synthetases (AARSs) are involved in several non-canonical processes such as apoptosis, inflammation and angiogenesis through their interactions with various cellular proteins. Nine of these AARSs interact with three aminoacyl-tRNA synthetase interacting multifunctional proteins (AIMPs), forming a multi-synthetase complex (MSC) in eukaryotes. Among the three AIMPs, AIMP2 is involved in controlling cell proliferation and apoptosis. However, a splicing variant of AIMP2 lacking exon 2, referred to as AIMP2-DX2, is oncogenic and compromises the pro-apoptotic activity of AIMP2 by competing with it for p53 and TRAF2...
February 7, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28179595/outcomes-of-totally-endoscopic-atrial-septal-defect-closure-using-a-glutaraldehyde-treated-autologous-pericardial-patch
#8
Hidefumi Nishida, Daisuke Nakatsuka, Yuji Kawano, Nobuhiko Hiraiwa, Shuichiro Takanashi, Minoru Tabata
BACKGROUND: We evaluated the outcomes of totally endoscopic minimally invasive surgery for atrial septal defect (ASD) using a glutaraldehyde-treated autologous pericardial patch in the transcatheter interventional era.Methods and Results:We retrospectively reviewed 37 consecutive patients who underwent totally endoscopic ASD closure with a glutaraldehyde-treated autologous pericardial patch between June 2011 and April 2015. All patients had been deferred from catheter-based intervention for clinical or anatomical reasons...
February 8, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28178887/magnesium-presence-prevents-removal-of-antigenic-nuclear-associated-proteins-from-bovine-pericardium-for-heart-valve-engineering
#9
Ailsa Dalgliesh, Zhi Zhao Liu, Leigh Griffiths
Current heart valve prostheses are associated with significant complications including aggressive immune response, limited valve life expectancy, and inability to grow in juvenile patients. Animal derived "tissue" valves undergo glutaraldehyde fixation to mask tissue antigenicity, however chronic immunological responses and associated calcification still commonly occur. A heart valve formed from an unfixed bovine pericardium (BP) extracellular matrix (ECM) scaffold, in which antigenic burden has been eliminated or significantly reduced, has potential to overcome deficiencies of current bio-prostheses...
February 8, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28157163/transmission-electron-microscopy-of-xdr-mycobacterium-tuberculosis-isolates-grown-on-high-dose-of-o%C3%AF-oxacin
#10
Mohammad Arjomandzadegan, Maryam Sadrnia, Leonid Titov, Larissa Surkova, Hossein Sarmadian, Reza Ghasemikhah, Hossein Hosseiny
The aim of the study was to investigate behavior of resistant Mycobacterium tuberculosis (MTB) isolates under a high dose of ofloxacin and its morphological changes. 19 extensively drug resistant (XDR) clinical isolates of MTB were grown on Löwenstein-Jensen medium containing progressively increasing concentrations of ofloxacin (2, 4, 8, 16, 32 mg/L). Ultra-structure analyses of resistant isolates grown on ofloxacin were conducted by transmission electron microscopy (TEM). Fixation was carried out by 4% glutaraldehyde in 0...
February 2, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28150361/cornea-and-its-adaptation-to-environment-and-accommodation-function-in-veiled-chameleon-chamaeleo-calyptratus-ultrastructure-and-3d-transmission-electron-tomography
#11
Saeed Akhtar, Adnan A Khan, Hussain A Albuhayzah, Turki M Almubrad
We investigate the ultrastructural features and 3D electron tomography of chameleon (Chamaeleon calyptratus) which is a native of desert environments of Saudi Arabia. The corneas of the chameleon were fixed in 2.5% glutaraldehyde containing cuprolinic blue in sodium acetate buffer for electron microscopy and tomography, and observed under a JEOL 1400 transmission electron microscope. The thin cornea (21.92 μm) contained 28-30 collagen fibril lamellae. The middle stromal lamellae (from 13 to 19) contained keratocytes with a long cell process and filled with granular material...
February 1, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28131109/fabrication-of-chitosan-silk-based-tracheal-scaffold-using-freeze-casting-method
#12
Zeinab Nematollahi, Mohammad Tafazzoli-Shadpour, Ali Zamanian, Amir Seyedsalehi, Shadmehr Mohammad-Behgam, Fariba Ghorbani, Fereshte Mirahmadi
Background: Since the treatments of long tracheal leisions are associated with some limitations, tissue enginered trachea is considered as an alternative option. This study aimed at preparing a composite scaffold, based on natural and synthetic materials for tracheal tissue engineering. Methods: Nine chitosan silk-based scaffolds were fabricated using three freezing rates (0.5, 1, and 2°C/min) and glutaraldehyde (GA) concentrations (0, 0.4, and 0.8 wt%). Samples were characterized, and scaffolds having mechanical properties compatible with those of human trachea and proper biodegradability were selected for chondrocyte cell seeding and subsequent biological assessments...
29, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28115131/applicability-of-live-dead-baclight-stain-with-glutaraldehyde-fixation-for-the-measurement-of-bacterial-abundance-and-viability-in-rainwater
#13
Wei Hu, Kotaro Murata, Daizhou Zhang
Rainwater contains substantial bacteria and rain is an efficient pathway for the dissemination of bacteria from the atmosphere to land and water surfaces. However, quantitative information on rainwater bacteria is very limited due to the lack of a reliable method. In this study, the epifluorescence microscopy enumeration with the LIVE/DEAD BacLight Bacterial Viability Kit stain was verified to quantify the abundance of viable and non-viable bacterial cells in rainwater, with the 4',6-diamidino-2-phenylindole (DAPI) stain for the reference of total cell counts...
January 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28101961/antibody-formation-towards-porcine-tissue-in-patients-implanted-with-crosslinked-heart-valves-is-directed-to-antigenic-tissue-proteins-and-%C3%AE-gal-epitopes-and-is-reduced-in-healthy-vegetarian-subjects
#14
Ulrike Böer, Falk F R Buettner, Ariane Schridde, Melanie Klingenberg, Samir Sarikouch, Axel Haverich, Mathias Wilhelmi
BACKGROUND: Glutaraldehyde-fixed porcine heart valves (ga-pV) are one of the most frequently used substitutes for insufficient aortic and pulmonary heart valves which, however, degenerate after 10-15 years. Yet, xeno-immunogenicity of ga-pV in humans including identification of immunogens still needs to be investigated. We here determined the immunogenicity of ga-pV in patients with respect to antibody formation, identity of immunogens and potential options to reduce antibody levels. METHODS: Levels of tissue-specific and anti-αGal antibodies were determined retrospectively in patients who received ga-pV for 51 months (n=4), 25 months (n=6) or 5 months (n=4) and compared to age-matched untreated subjects (n=10) or younger subjects with or without vegetarian diet (n=12/15)...
January 18, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28099453/elimination-of-porcine-epidemic-diarrhea-virus-in-an-animal-feed-manufacturing-facility
#15
Anne R Huss, Loni L Schumacher, Roger A Cochrane, Elizabeth Poulsen, Jianfa Bai, Jason C Woodworth, Steve S Dritz, Charles R Stark, Cassandra K Jones
Porcine Epidemic Diarrhea Virus (PEDV) was the first virus of wide scale concern to be linked to possible transmission by livestock feed or ingredients. Measures to exclude pathogens, prevent cross-contamination, and actively reduce the pathogenic load of feed and ingredients are being developed. However, research thus far has focused on the role of chemicals or thermal treatment to reduce the RNA in the actual feedstuffs, and has not addressed potential residual contamination within the manufacturing facility that may lead to continuous contamination of finished feeds...
2017: PloS One
https://www.readbyqxmd.com/read/28092004/bio-based-degradation-of-emerging-endocrine-disrupting-and-dye-based-pollutants-using-cross-linked-enzyme-aggregates
#16
Muhammad Bilal, Muhammad Asgher, Hafiz M N Iqbal, Hongbo Hu, Xuehong Zhang
In this study, manganese peroxidase (MnP) from an indigenous white-rot fungus Ganoderma lucidum IBL-05 was insolubilized in the form of cross-linked enzyme aggregates (CLEAs) using various aggregating agents, i.e., acetone, ammonium sulfate, ethanol, 2-propanol, and tert-butanol, followed by glutaraldehyde (GA) cross-linking. The precipitant type, MnP, and GA concentrations affected the CLEAs activity recovery and aggregation yield. Among precipitants used, acetone appeared to be the most efficient aggregation agent, providing the highest activity recovery and aggregation yield of 31...
January 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28068870/induced-remodeling-of-porcine-tendons-to-human-anterior-cruciate-ligaments-by-%C3%AE-gal-epitope-removal-and-partial-crosslinking
#17
Kevin Robert Stone, Ann Walgenbach, Uri Galili
This review describes a novel method developed for processing porcine tendon and other ligament implants which enables in situ remodeling into autologous ligaments in humans. The method differs from methods using extracellular matrices (ECM) which provide post-operative ortho-biologic support (i.e. augmentation grafts) for healing of injured ligaments, in that the porcine bone-patellar-tendon-bone itself serves as the graft replacing ruptured anterior cruciate ligament (ACL). The method allows for gradual remodeling of porcine tendon into autologous human ACL while maintaining the biomechanical integrity...
January 9, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28063018/analysis-of-the-variation-in-low-level-laser-energy-density-on-the-crushed-sciatic-nerves-of-rats-a-morphological-quantitative-and-morphometric-study
#18
Eduardo Keiske Mastuda Ziago, Valéria Paula Sassoli Fazan, Mamie Mizusaki Iyomasa, Luiz Gustavo Sousa, Paula Yumi Yamauchi, Eunice Aparecida da Silva, Eduardo Borie, Ramón Fuentes, Fernando José Dias
The objective of this study was to evaluate three energy densities of low-level laser therapy (LLLT, GaAlAs, 780 nm, 40 mW, 0.04 cm(2)) for the treatment of lesions to peripheral nerves using the sciatic nerve of rats injured via crushing model (15 kgf, 5.2 MPa). Thirty Wistar rats (♂, 200-250 g) were divided into five groups (n = 6): C-control, not injured, and irradiated; L0-injured nerve without irradiation; L4-injured nerve irradiated with LLLT 4 J/cm(2) (0.16 J); L10-injured nerve irradiated with LLLT 10 J/cm(2) (0...
February 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28044425/immediate-effects-of-diamond-burr-debridement-in-patients-with-spontaneous-chronic-corneal-epithelial-defects-light-and-electron-microscopic-evaluation
#19
Charlotte Dawson, Carolina Naranjo, Belen Sanchez-Maldonado, Georgina V Fricker, Rose N Linn-Pearl, Natalia Escanilla, Christiane Kafarnik, David J Gould, Rick F Sanchez, Marian Matas-Riera
PURPOSE: To evaluate immediate effects of diamond burr debridement (DBD) on the cornea of canine patients diagnosed with spontaneous chronic corneal epithelial defects (SCCEDs). ANIMALS STUDIED: Eight client owned dogs with SCCEDs. METHODS: Nine eyes from eight dogs with SCCEDs underwent superficial keratectomy (SK). The ulcerated area was divided into quadrants with a 300-micron restricted depth knife. Two of four quadrants underwent DBD for 40-60 s...
January 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28033559/multiple-signal-amplification-strategies-for-ultrasensitive-label-free-electrochemical-immunoassay-for-carbohydrate-antigen-24-2-based-on-redox-hydrogel
#20
Zhongxue Tang, Yuanyuan Fu, Zhanfang Ma
In this work, multiple signal amplification strategies for ultrasensitive label-free electrochemical immunoassay for carbohydrate antigen 24-2 (CA242) were developed using redox sodium alginate-Pb(2+)-graphene oxide (SA-Pb(2+)-GO) hydrogel. The SA-Pb(2+)-GO hydrogel was synthesised by simply mixing SA, GO, and Pb(2+) and then implemented as a novel redox species with a strong current signal at -0.46V (vs. Ag/AgCl). After the three-dimensional and porous SA-Pb(2+)-GO hydrogel was in situ generated on a glassy carbon electrode (GCE), chitosan was adsorbed on the obtained electrode to further enrich Pb(2+)...
December 22, 2016: Biosensors & Bioelectronics
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