keyword
MENU ▼
Read by QxMD icon Read
search

Glutaraldehyde 2%

keyword
https://www.readbyqxmd.com/read/29346675/in-search-of-the-best-xenogeneic-material-for-a-paediatric-conduit-an-experimental-study
#1
Irina Y Zhuravleva, Nataliya R Nichay, Yuriy Y Kulyabin, Tatyana P Timchenko, Alexander A Korobeinikov, Yuliya F Polienko, Svetlana S Shatskaya, Elena V Kuznetsova, Alexey V Voitov, Alexander V Bogachev-Prokophiev, Alexander M Karaskov
OBJECTIVES: The development of calcification-resistant bioprosthetic materials is a very important challenge for paediatric surgery. The subcutaneous implantation in rats is the well-known first-stage model for this kind of research. Using this model, we aimed to compare calcification of the porcine aortic wall and bovine pericardium and jugular vein wall cross-linked with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (DE). We also determined the efficacy of DE-preserved tissue modification with 2-(2-carboxyethylamino)ethylidene-1,1-bisphosphonic acid (CEABA)...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29321261/a-model-free-method-for-measuring-dimerization-free-energies-of-clc-ec1-in-lipid-bilayers
#2
Rahul Chadda, Lucy Cliff, Marley Brimberry, Janice L Robertson
The thermodynamic reasons why membrane proteins form stable complexes inside the hydrophobic lipid bilayer remain poorly understood. This is largely because of a lack of membrane-protein systems amenable for equilibrium studies and a limited number of methods for measuring these reactions. Recently, we reported the equilibrium dimerization of the CLC-ec1 Cl-/H+ transporter in lipid bilayers (Chadda et al. 2016. eLife https://doi.org/10.7554/eLife.17438), which provided a new type of model system for studying protein association in membranes...
January 10, 2018: Journal of General Physiology
https://www.readbyqxmd.com/read/29310854/stabilization-of-dimeric-%C3%AE-glucosidase-from-aspergillus-niger-via-glutaraldehyde-immobilization-under-different-conditions
#3
Perla Guadalupe Vazquez-Ortega, Maria Teresa Alcaraz-Fructuoso, Juan A Rojas-Contreras, Javier López-Miranda, Roberto Fernandez-Lafuente
The dimeric enzyme β-glucosidase from Aspergillus niger has been immobilized on different amino-agarose beads at pH 5 and 7, exploiting the versatility of glutaraldehyde. The stability of the free enzyme depended on enzyme concentration. Immobilization via ion exchange improved enzyme stability/activity, depending on the immobilization pH. However, the enzyme was desorbed in 75 mM NaCl at pH 7 and some stability/enzyme concentration dependence still existed. TREATMENT: of these biocatalysts with glutaraldehyde increased enzyme stability (e...
March 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29310852/enhancement-of-n-3-polyunsaturated-fatty-acid-glycerides-in-sardine-oil-by-a-bioimprinted-cross-linked-candida-rugosa-lipase
#4
Charanyaa Sampath, Prasanna D Belur, Regupathi Iyyasami
Considering the advantages of bioimprinting and carrier free immobilization, cross-linked enzyme aggregates (CLEA) were prepared by using bioimprinted Candida rugosa lipase (CRL) with Bovine serum albumin (BSA), Polyethyleneimine and glutaraldehyde. Effect of various factors such as CRL-Oleic acid ratio, CRL-BSA ratio, CRL- Polyethyleneimine ratio, glutaraldehyde loading, cross-linking time etc., on lipase activity recovery and aggregate yield were studied and optimized. This immobilized lipase (CRL-CLEA) was used for the selective hydrolysis of ester linkages of non-PUFA glycerides, with an aim to concentrate EPA and DHA glycerides in the Sardine oil...
March 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29295656/core-shell-poly-lactide-co-%C3%AE%C2%B5-caprolactone-gelatin-fiber-scaffolds-as-ph-sensitive-drug-delivery-systems
#5
Qingqing Sang, Heyu Li, Gareth Williams, Huanling Wu, Li-Min Zhu
Dual-drug-loaded pH-responsive fiber scaffolds were successfully prepared by coaxial electrospinning. These were designed with the aim of being sutured into the resection site after tumor removal, to aid recovery and prevent cancer recurrence. The shell was made up of a mixture of gelatin and sodium bicarbonate (added to provide pH-sensitivity), and was loaded with the anti-inflammatory drug ciprofloxacin; the core comprised poly(lactide-co-ε-caprolactone) with the chemotherapeutic doxorubicin hydrochloride...
January 1, 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29281685/nanoparticle-size-and-production-efficiency-are-affected-by-the-presence-of-fatty-acids-during-albumin-nanoparticle-fabrication
#6
Christian C Luebbert, Tessa M Clarke, Roberta Pointet, Grant E Frahm, Sharon Tam, Barry Lorbetskie, Simon Sauvé, Michael J W Johnston
We have previously identified extensive glycation, bound fatty acids and increased quantities of protein aggregates in commercially available recombinant HSA (rHSA) expressed in Oryza sativa (Asian rice) (OsrHSA) when compared to rHSA from other expression systems. We propose these differences may alter some attributes of nanoparticles fabricated with OsrHSA, as studies have associated greater quantities of aggregates with increased nanoparticle diameters. To determine if this is the case, nanoparticles were fabricated with OsrHSA from various suppliers using ethanol desolvation and subsequent glutaraldehyde cross-linking...
2017: PloS One
https://www.readbyqxmd.com/read/29273368/enhanced-vascular-regeneration-with-chemically-physically-treated-bovine-human-pericardium-in-rodents
#7
Mathieu van Steenberghe, Thomas Schubert, Daela Xhema, Caroline Bouzin, Yves Guiot, Jérôme Duisit, Karim Abdelhamid, Pierre Gianello
BACKGROUND: Glutaraldehyde-treated pericardia for cardiovascular applications have poor long-term clinical results. The efficacy of a combined physical/chemical treatment to improve pericardium biocompatibility and vascular regeneration was assessed and compared with detergent treatment and two commercial bovine pericardia: PeriGuard (DGBP) and Edwards pericardium (nDGBP). The physical and chemical process was applied to bovine and human pericardia (DBP-DHP), and the detergent process was applied to bovine (DDBP)...
February 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29230223/glutaraldehyde-cross-linking-of-hiv-1-env-trimers-skews-the-antibody-subclass-response-in-mice
#8
Martina Soldemo, Monika Àdori, Julian M Stark, Yu Feng, Karen Tran, Richard Wilson, Lifei Yang, Javier Guenaga, Richard T Wyatt, Gunilla B Karlsson Hedestam
Well-ordered soluble HIV-1 envelope glycoprotein (Env) spike mimetics such as Native Flexibly Linked (NFL) trimers display high homogeneity, desired antigenicity, and high in vitro stability compared to previous generation soluble HIV-1 Env trimers. Glutaraldehyde (GLA) cross-linking was shown to further increase the thermostability of clade C 16055 NFL trimers and enhance the induction of tier 2 autologous neutralizing antibodies in guinea pigs. Here, we investigated if GLA fixation affected other aspects of the Env-specific immune response by performing a comparative immunogenicity study in C57BL/6 mice with non-fixed and GLA-fixed 16055 NFL trimers administered in AbISCO-100 adjuvant...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29226612/glutaraldehyde-cross-linking-increases-the-stability-of-lumbricus-terrestris-erythrocruorin
#9
Athul Rajesh, Devon Zimmerman, Kyle Spivack, Osheiza Abdulmalik, Jacob Elmer
Since donated red blood cells must be constantly refrigerated, they are not available in remote areas and battlefields. We have previously shown that the hemoglobin of the earthworm Lumbricus terrestris (LtEc) is an effective and safe substitute for donated blood that is stable enough to be stored for long periods at the relatively high temperatures that may be encountered in remote areas. The goal of this study was to increase the thermal stability of LtEc by covalently cross-linking LtEc with glutaraldehyde (gLtEc)...
December 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29214017/morphological-bactericidal-fast-acting-effects-of-peracetic-acid-a-high-level-disinfectant-against-staphylococcus-aureus-and-pseudomonas-aeruginosa-biofilms-in-tubing
#10
T Chino, Y Nukui, Y Morishita, K Moriya
Background: The bactericidal effect of disinfectants against biofilms is essential to reduce potential endoscopy-related infections caused by contamination. Here, we investigated the bactericidal effect of a high-level disinfectant, peracetic acid (PAA), against Staphylococcus aureus and Pseudomonas aeruginosa biofilm models in vitro. Methods: S. aureus and P. aeruginosa biofilms were cultured at 35 °C for 7 days with catheter tubes. The following high-level disinfectants (HLDs) were tested: 0...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29205950/biodegradable-nanoparticles-enhanced-adhesiveness-of-mussel-like-hydrogels-at-tissue-interface
#11
Nikhil Pandey, Amirhossein Hakamivala, Cancan Xu, Prashant Hariharan, Boris Radionov, Zhong Huang, Jun Liao, Liping Tang, Philippe Zimmern, Kytai T Nguyen, Yi Hong
Popular bioadhesives, such as fibrin, cyanoacrylate, and albumin-glutaraldehyde based materials, have been applied for clinical applications in wound healing, drug delivery, and bone and soft tissue engineering; however, their performances are limited by weak adhesion strength and rapid degradation. In this study a mussel-inspired, nanocomposite-based, biodegradable tissue adhesive is developed by blending poly(lactic-co-glycolic acid) (PLGA) or N-hydroxysuccinimide modified PLGA nanoparticles (PLGA-NHS) with mussel-inspired alginate-dopamine polymer (Alg-Dopa)...
December 4, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29195822/effect-of-crosslinkers-on-bond-strength-stability-of-fiber-posts-to-root-canal-dentin-and-in-situ-proteolytic-activity
#12
Juliana R L Alonso, Fernanda G Basso, Débora L S Scheffel, Carlos Alberto de-Souza-Costa, Josimeri Hebling
STATEMENT OF PROBLEM: Improved stability of the adhesive interface can be obtained using crosslinkers. However, research on the use of crosslinkers in root dentin is lacking. PURPOSE: The purpose of this in vitro study was to evaluate the effect of crosslinkers on the proteolytic activity of root dentin and on the bond strength of resin-cemented fiber posts. MATERIAL AND METHODS: Single root canals were obtained from premolars (n=48) and endodontically treated before being divided into 4 groups: deionized water (control), 0...
November 29, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/29193844/sodium-bicarbonate-gelled-chitosan-beads-as-mechanically-stable-carriers-for-the-covalent-immobilization-of-enzymes
#13
Marwa I Wahba
The poor mechanical stability of chitosan has long impeded its industrial utilization as an immobilization carrier. In this study, the mechanical properties of chitosan beads were greatly improved through utilizing the slow rate of the sodium bicarbonate-induced chitosan gelation and combining it with the chemical cross-linking action of glutaraldehyde (GA). The GA-treated sodium bicarbonate-gelled chitosan beads exhibited much better mechanical properties and up to 2.45-fold higher observed activity of the immobilized enzyme (β-D-galactosidase (β-gal)) when compared to the GA-treated sodium tripolyphosphate (TPP)-gelled chitosan beads...
November 29, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29184106/burkholderia-cepacia-lipase-immobilized-on-heterofunctional-magnetic-nanoparticles-and-its-application-in-biodiesel-synthesis
#14
Kai Li, Yanli Fan, Yaojia He, Leping Zeng, Xiaotao Han, Yunjun Yan
Biodiesel production using immobilized lipase as a biocatalyst is a promising process. The performance of immobilized lipase is mainly determined by supporting materials and immobilization method. To avoid the shortcomings of adsorption and covalent bonding methods, in this study, we developed a novel heterofunctional carrier of being strengthened anion exchange and weakened covalent binding to avoid activity loss and improve operational stability of the immobilized lipase. 2,3-epoxypropyltrimethylammonium chloride with epoxy and quaternary ammonium group and glutaraldehyde were grafted onto aminated magnetic nanoparticles (AMNPs) to generate a new matrix, named GEAMNP...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29178702/clinical-efficacy-of-resin-based-materials-for-dentin-hypersensitivity-treatment
#15
Gabriela D Canali, Sergio A Ignacio, Rodrigo N Rached, Evelise M Souza
PURPOSE: To evaluate the effect of three resin-based materials in the treatment of dentin hypersensitivity (DH) up to a 6-month follow-up. METHODS: 30 participants (179 teeth) with DH were enrolled in this randomized, double-blind, controlled clinical study. The teeth were randomly allocated into one of three groups: CV (resin-modified glass ionomer varnish - Clinpro XT Varnish), CP (fluoride-containing self-etch adhesive - Clearfil SE Protect) and GB (glutaraldehyde-containing etch-and-rinse adhesive - Gluma 2 Bond)...
August 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29164856/aerosol-crosslinked-crown-ether-diols-melded-with-poly-vinyl-alcohol-as-specialized-microfibrous-li-adsorbents
#16
Lawrence A Limjuco, Grace Masbate Nisola, Rey Eliseo Castillo Torrejos, Jeong Woo Han, Ho Seong Song, Khino J Parohinog, Sangho Koo, Seong-Poong Lee, Wook-Jin Chung
Crown ether (CE)-based Li(+) adsorbent microfibers (MFs) were successfully fabricated through a combined use of CE diols, electrospinning, and aerosol crosslinking. The 14-16 membered CEs, with varied ring sub-units and cavity dimensions, have two hydroxyl groups for covalent attachments to poly(vinyl alcohol) (PVA) as the chosen matrix. The CE diols were blended with PVA and transformed into microfibers via electrospinning, a highly effective technique in minimizing CE loss during MF fabrication. Subsequent aerosol glutaraldehyde (GA) crosslinking of the electrospun CE/PVA MFs stabilized the adsorbents in water...
November 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29158128/fullerene-pamam-g5-composite-modified-impedimetric-biosensor-to-detect-fetuin-a-in-real-blood-samples
#17
Zihni Onur Uygun, Çağdaş Şahin, Merve Yilmaz, Yasemin Akçay, Ali Akdemir, Ferhan Sağin
The aim of this study is to develop a nanomaterial-dendrimer composite modified biosensor to detect Fetuin-A (HFA) in real blood samples. For this purpose, we designed an Electrochemical Impedance Spectroscopy (EIS) based anti-Fetuin-A (Anti-HFA) modified biosensor system and tested in real blood samples. Chronoimpedance was also employed. The same samples were analyzed with ELISA and the results were compared for validation of the new system. Gold screen printed electrodes (AuSPE) were used as transducer. Firstly, a self-assembly monolayer (SAM) was formed on gold surface by 4-aminothiophenol (4-ATP), Fullerene and PAMAM-NH2 (G5), layers were formed, consecutively...
November 17, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29157155/work-environment-and-occupational-risk-assessment-for-small-animal-portuguese-veterinary-activities
#18
Angela C Macedo, Vânia T Mota, João M Tavares, Osvaldo L Machado, Francisco X Malcata, Marinela P Cristo, Olga N Mayan
The professional work of small animal veterinary staff encompasses a wide diversity of demanding tasks. This has prompted a number of studies covering physical, chemical, biological, ergonomic or psychological hazards, as well as their health effects upon veterinary workers. However, such results were obtained from self-reported surveys (via paper or online). This study reports the identification of potential hazards and provides a risk assessment of 15 veterinary clinics based on data from walk-through surveys, interviews with workers, and quantification of indoor air quality parameters including concentration of volatile organic compounds (total, isoflurane and glutaraldehyde)...
November 20, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/29154787/functionalization-of-paramagnetic-nanoparticles-for-protein-immobilization-and-purification
#19
Lara A B C Carneiro, Richard J Ward
A paramagnetic nanocomposite coated with chitosan and N-(5-Amino-1-carboxy-pentyl) iminodiacetic acid (NTA) that is suitable for protein immobilization applications has been prepared and characterized. The nanoparticle core was synthesized by controlled aggregation of Fe3O4 under alkaline conditions, and Transmission Electron Microscopy revealed a size distribution of 10-50 nm. The nanoparticle core was coated with chitosan and derivatized with glutaraldehyde and NTA, as confirmed by Fourier Transform Infrared Spectroscopy...
November 18, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29137115/cost-effective-and-handmade-paper-based-immunosensing-device-for-electrochemical-detection-of-influenza-virus
#20
Sivaranjani Devarakonda, Renu Singh, Jyoti Bhardwaj, Jaesung Jang
Although many studies concerning the detection of influenza virus have been published, a paper-based, label-free electrochemical immunosensor has never been reported. Here, we present a cost-effective, handmade paper-based immunosensor for label-free electrochemical detection of influenza virus H1N1. This immunosensor was prepared by modifying paper with a spray of hydrophobic silica nanoparticles, and using stencil-printed electrodes. We used a glass vaporizer to spray the hydrophobic silica nanoparticles onto the paper, rendering it super-hydrophobic...
November 11, 2017: Sensors
keyword
keyword
43034
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"