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Glutaraldehyde 2%

Karthikumar Sankar, Anant Achary
The lipophilic antioxidants, glyceryl ferulate and feruloyl glyceryl linoleate, were synthesized using lipase from Bacillus subtilis AKL 13. The extracellular lipase was produced by cultivation of the strain in modified minimal medium and the enzyme was recovered by fractionation at 80% ammonium salt saturation. The concentrated enzyme with the specific activity of (4647±66) U/mg was immobilized on Celite® 545 and crosslinked using glutaraldehyde. The prepared enzyme catalyst was used for esterification of ferulic and linoleic acids with glycerol separately in hexane butane solvent system at 50 °C and 3...
December 2017: Food Technology and Biotechnology
Aliyeh Yousefi Abdolmaleki, Hamid Zilouei, Saied Nouri Khorasani, Kiomars Zargoosh
In this work, the preparation and characterization of glutaraldehyde-crosslinked electrospun nanofibers of chitosan/poly(vinyl alcohol) (GCCPN) as a new adsorbent for tetracycline (TC) is reported. Electrospun nanofibers of chitosan/poly(vinyl alcohol) (PVA) were prepared by employing a 75:25 volumetric ratio of chitosan:PVA, voltage of 30 kV, collection distance of 10 cm, and injection flow rate of 2 mL/h. Then, the nanofibers were crosslinked via applying the glutaraldehyde on them for 3 h at 40 °C. The nanofibers were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction...
March 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Eda Yuhana Ariffin, Yook Heng Lee, Dedi Futra, Ling Ling Tan, Nurul Huda Abd Karim, Nik Nuraznida Nik Ibrahim, Asmat Ahmad
A novel electrochemical DNA biosensor for ultrasensitive and selective quantitation of Escherichia coli DNA based on aminated hollow silica spheres (HSiSs) has been successfully developed. The HSiSs were synthesized with facile sonication and heating techniques. The HSiSs have an inner and an outer surface for DNA immobilization sites after they have been functionalized with 3-aminopropyltriethoxysilane. From field emission scanning electron microscopy images, the presence of pores was confirmed in the functionalized HSiSs...
March 5, 2018: Analytical and Bioanalytical Chemistry
Akikazu Sakudo, Hirotoshi Miyagi, Takaya Horikawa, Risa Yamashiro, Tatsuya Misawa
Gastrointestinal endoscopy is an important tool for the indentification and treatment of disorders of the gastrointestinal tract. However, nosocomial infections of Helicobacter pylori have been linked to the use of contaminated endoscopes. Disinfectants such as glutaraldehyde, ortho-phthalaldehyde and peracetic acid are generally used in the reprocesssing of endoscopes, but these chemicals are hazardous to human health. Thus, safer reprocessing and disinfecion methods are needed. In this study, we applied a dielectric barrier discharge (DBD) plasma torch for inactivation of H...
February 19, 2018: Chemosphere
S Cavelier, A K Dastjerdi, M D McKee, F Barthelat
The most prominent structural components in bone are collagen and mineral. However, bone additionally contains a substantial amount of noncollagenous proteins (most notably of the SIBLING protein family), some of which may act as cohesive/adhesive "binders" for the composite hybrid collagen/mineral scaffolding, whether in the bulk phase of bone, or at its interfaces. One such noncollagenous protein - osteopontin (OPN) - appears to be critical to the deformability and fracture toughness of bone. In the present study, we used a reconstructed synthetic mineral-OPN-mineral interface, and a biogenic (natural tooth dentin) mineral/collagen-OPN-mineral/collagen interface, to measure the fracture toughness of OPN on mineralized substrates...
February 24, 2018: Bone
K Ozlem, G M Esad, A Ayse, U Aslihan
Aim: The aim of this clinical study was to determine and compare the efficiency of the glutaraldehyde-containing agent (GCA), Nd:YAG, Er,Cr:YSGG lasers, and the combination of them on the dentin hypersensitivity (DH) treatment. Subjects and Methods: This study was performed with the participation of 17 healthy adult patients having 100 teeth with DH; the patients were randomly divided into five groups according to the treatment protocol: (1) application of GCA on sensitive teeth, (2) Nd:YAG laser (1 W/cm2 , 10 Hz) irradiation on sensitive teeth, (3) application of GCA on sensitive teeth and then Nd:YAG laser irradiation, (4) Er,Cr:YSGG laser (0...
February 2018: Nigerian Journal of Clinical Practice
Shintaro Nemoto, Hayato Konishi, Ryo Shimada, Tatsuya Suzuki, Takahiro Katsumata, Hideaki Yamada, Jun Sakurai, Yohei Sakamoto, Kazuteru Kohno, Atsuko Onishi, Masaya Ito
OBJECTIVES: Materials used in paediatric cardiac surgery have drawbacks of deterioration, calcification and pseudointimal proliferation resulting in haemodynamic disturbance. The aim of this study was to investigate whether these drawbacks can be overcome by in situ tissue regeneration using a newly developed synthetic hybrid fabric (SHF). METHODS: The SHF is an expandable, warp-knitted fabric composed of a combination of biodegradable [poly-l-lactic acid (PLLA)] and non-biodegradable (polyethylene terephthalate) yarns...
February 14, 2018: European Journal of Cardio-thoracic Surgery
Walaa A Abdel Wahab, Eman A Karam, Mohammed E Hassan, Amany L Kansoh, Mona A Esawya, Ghada E A Awad
Pectinase produced by a honey derived from the fungus Aspergillus awamori KX943614 was covalently immobilized onto gel beads made of alginate and agar. Polyethyleneimine, glutaraldehyde, loading time and enzyme's units were optimized by 24 full factorial central composite design (CCD). The immobilization process increased the optimal working pH for the free pectinase from 5 to a broader range of pH 4.5-5.5 and the optimum operational temperature from 55 °C to a higher temperature, of 60 °C, which is favored to reduce the enzyme's microbial contamination...
February 16, 2018: International Journal of Biological Macromolecules
Nataliya R Nichay, Irina Y Zhuravleva, Yuriy Y Kulyabin, Tatyana P Timchenko, Alexey V Voitov, Elena V Kuznetsova, Ilia A Soynov, Alexey V Zubritskiy, Alexander V Bogachev-Prokophiev, Alexander M Karaskov
OBJECTIVES: In this study, we aimed to determine the incidence of reintervention and calcification of xenografts in paediatric patients who underwent placement of the right ventricle-to-pulmonary artery valved conduits. METHODS: We retrospectively analysed clinical data of paediatric patients (1 day-18 years) who underwent right ventricular outflow tract reconstruction using xenograft from 2000 to 2016 at a single centre. RESULTS: A total of 301 patients underwent the placement of 337 xenografts, including glutaraldehyde-treated bovine jugular vein (n = 171, 50...
February 14, 2018: Interactive Cardiovascular and Thoracic Surgery
Oliver Reuthebuch, Luca Koechlin, Ulrich Schurr, Martin Grapow, Jens Fassl, Friedrich S Eckstein
OBJECTIVE: To assess the clinical implementation and report preliminary results of a novel technique called the Ozaki procedure for stentless aortic valve replacement through reconstruction of the valve leaflets from autologous pericardium. METHOD: Between September 2015 and May 2017 30 patients (20 males, mean ± standard deviation age 66.83 ± 10.55 years) suffering from aortic stenosis (AS, n = 7), aortic regurgitation (AR, n = 12), or a combination of both (AS/AR, n = 11) were assigned for an Ozaki procedure...
February 14, 2018: Swiss Medical Weekly
Sibelli Olivieri Parreiras, Anna Luiza Szesz, Fabiana Madalozzo Coppla, Eveline Claudia Martini, Paulo Vitor Farago, Alessandro D Loguercio, Alessandra Reis
BACKGROUND: In this randomized study, split-mouth, triple-blind clinical trial, the authors evaluated the efficacy of a desensitizing gel that contained 5% potassium nitrate and 5% glutaraldehyde applied before in-office bleaching with 35% hydrogen peroxide (HP). METHODS: Treatment with the desensitizing or placebo control gels was randomly assigned to one-half of the maxillary teeth of 42 patients in a split-mouth design. The desensitizing gels were applied and maintained in contact with the tooth enamel for 10 minutes, followed by 2 HP bleaching sessions separated by 1 week...
February 10, 2018: Journal of the American Dental Association
Peter A Bain, Anu Kumar
The widespread use of hydraulic fracturing (HF) in oil and gas extraction operations has led to concern over environmental risks posed by chemicals used in HF fluids. Here we employed a suite of stable luciferase reporter gene assays to investigate the potential for selected HF chemicals or geogenics to activate or antagonise nuclear receptor signalling. We screened three biocides (bronopol [BP], glutaraldehyde [GA], and tetrakis(hydroxymethyl)phosphonium sulfate [THPS]), a surfactant (2-butoxyethanol), a friction reducer (polyacrylamide), and a coal seam geogenic (o-cresol) for their potential to act as agonists or antagonists of the estrogen receptor, androgen receptor, progesterone receptor (PR), glucocorticoid receptor or peroxisome proliferator-activated receptor gamma (PPARγ)...
January 9, 2018: Chemosphere
B Gao, X J Li, M Lin, Y Y Li, Y Dong
Objective: To evaluate the application effect of nanofiber chitosan-collagen membrane (NCM) on guided bone regeneration (GBR). Methods: The mixture of collagen, chitosan, polyethylene oxide was used to make up the NCM by electrospinning, then the NCM was crosslinked by glutaraldehyde vapor. The physical property of the NCM was measured by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). MC3T3-E1 osteoblasts were cultured on NCM to characterize the biocompatibility. The effectiveness of four groups [contrast group, Bio-gide membrane (BGM), compressed chitosan-collagen menbrane (CCM), NCM/CCM] on bone regeneration were evaluated in critical-sized defects (diameter=5 mm) in SD rats...
February 9, 2018: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Yang Zhang, Juan Xu
This paper proposes a novel type of multifunctional envelope-type mesoporous silica nanoparticle (MSN) to achieve cancer cell targeting and drug-controlled release. In this system, MSNs were first modified by active targeting moiety hyaluronic acid (HA) for breast cancer cell targeting and hyaluronidases (Hyal)-induced intracellular drug release. Then gelatin, a proteinaceous biopolymer, was grafted onto the MSNs to form a capping layer via glutaraldehyde-mediated cross-linking. To shield against unspecific uptake of cells and prolong circulation time, the nanoparticles were further decorated with poly(ethylene glycol) polymers (PEG) to obtain MSN@HA-gelatin-PEG (MHGP)...
January 2018: Royal Society Open Science
Samia A Ahmed, Faten A Mostafa, Mona A Ouis
α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer. Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage. Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design)...
January 30, 2018: International Journal of Biological Macromolecules
Xueshi Luo, Zhenzhao Guo, Ping He, Tian Chen, Lihua Li, Shan Ding, Hong Li
Collagen electrospun scaffolds properly reproduce the framework of the extracellular matrix (ECM) of tissues that are natural with the fibrous morphology of the protein by coupling large biomimetism of the biological material. However, traditional solvents employed for collagen electrospinning lead to poor mechanical attributes and bad hydro-stability. In this work, by N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride with N-hydroxysulfosuccinimide (EDC-NHS), glutaraldehyde (GTA) and genipin (GP) respectively, electrospun collagen fibers cross-linked, effectively stabilized the fiber morphology over 2 months and improved the mechanical properties in both dry and wet state, especially EDC-NHS with large ultimate tensile stress and εb...
January 29, 2018: International Journal of Biological Macromolecules
Katlin I Mallette, Peter Pieroni, Sonny S Dhalla
AIM: To correlate the length of endoscope hang time and number of bacteria cultured prior to use. METHODS: Prospectively, we cultured specimens from 19 gastroscopes, 24 colonoscopes and 5 side viewing duodenoscopes during the period of 2011 to 2015. A total of 164 results had complete data denoting date of cleansing, number of days stored and culture results. All scopes underwent initial cleaning in the endoscopy suite utilizing tap water, and then manually cleaned and flushed...
January 16, 2018: World Journal of Gastrointestinal Endoscopy
William M L Neethling, Kirsten Puls, Alethea Rea
OBJECTIVES: Durability of bioscaffolds cross-linked with glutaraldehyde and used in cardiovascular surgery is limited by biomechanical instability, calcification and reduced biocompatibility. This study compares CardioCel®, a bovine pericardial scaffold engineered via the ADAPT® process to ensure optimized biostability and biocompatibility, with the commonly used bioscaffolds. METHODS: Bovine pericardial scaffolds, cross-linked with 0.6% glutaraldehyde (XenoLogiX™, PeriGuard®), dye-mediated photo-oxidized (PhotoFix™) and a non-crosslinked porcine scaffold (CorMatrix®), were compared with CardioCel (decellularized, cross-linked with 0...
January 18, 2018: Interactive Cardiovascular and Thoracic Surgery
Irina Y Zhuravleva, Nataliya R Nichay, Yuriy Y Kulyabin, Tatyana P Timchenko, Alexander A Korobeinikov, Yuliya F Polienko, Svetlana S Shatskaya, Elena V Kuznetsova, Alexey V Voitov, Alexander V Bogachev-Prokophiev, Alexander M Karaskov
OBJECTIVES: The development of calcification-resistant bioprosthetic materials is a very important challenge for paediatric surgery. The subcutaneous implantation in rats is the well-known first-stage model for this kind of research. Using this model, we aimed to compare calcification of the porcine aortic wall and bovine pericardium and jugular vein wall cross-linked with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (DE). We also determined the efficacy of DE-preserved tissue modification with 2-(2-carboxyethylamino)ethylidene-1,1-bisphosphonic acid (CEABA)...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
Rahul Chadda, Lucy Cliff, Marley Brimberry, Janice L Robertson
The thermodynamic reasons why membrane proteins form stable complexes inside the hydrophobic lipid bilayer remain poorly understood. This is largely because of a lack of membrane-protein systems amenable for equilibrium studies and a limited number of methods for measuring these reactions. Recently, we reported the equilibrium dimerization of the CLC-ec1 Cl-/H+ transporter in lipid bilayers (Chadda et al. 2016. eLife, which provided a new type of model system for studying protein association in membranes...
January 10, 2018: Journal of General Physiology
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