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https://www.readbyqxmd.com/read/29790879/exploring-matrix-effects-and-quantifying-organic-additives-in-hydraulic-fracturing-associated-fluids-using-liquid-chromatography-electrospray-ionization-mass-spectrometry
#1
Marika Nell, Damian E Helbling
Hydraulic fracturing (HF) operations utilize millions of gallons of water amended with chemical additives including biocides, corrosion inhibitors, and surfactants. Fluids injected into the subsurface return to the surface as wastewaters, which contain a complex mixture of additives, transformation products, and geogenic chemical constituents. Quantitative analytical methods are needed to evaluate wastewater disposal alternatives or to conduct adequate exposure assessments. However, our narrow understanding of how matrix effects change the ionization efficiency of target analytes limits the quantitative analysis of polar to semi-polar HF additives by means of liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS)...
May 23, 2018: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/29787347/stp-position-paper-recommended-best-practices-for-sampling-processing-and-analysis-of-the-peripheral-nervous-system-nerves-and-somatic-and-autonomic-ganglia-during-nonclinical-toxicity-studies
#2
Brad Bolon, Georg Krinke, Mark T Butt, Deepa B Rao, Ingrid D Pardo, Bernard S Jortner, Robert H Garman, Karl Jensen, Lydia Andrews-Jones, James P Morrison, Alok K Sharma, Michael S Thibodeau
Peripheral nervous system (PNS) toxicity is surveyed inconsistently in nonclinical general toxicity studies. These Society of Toxicologic Pathology "best practice" recommendations are designed to ensure consistent, efficient, and effective sampling, processing, and evaluation of PNS tissues for four different situations encountered during nonclinical general toxicity (screening) and dedicated neurotoxicity studies. For toxicity studies where neurotoxicity is unknown or not anticipated (situation 1), PNS evaluation may be limited to one sensorimotor spinal nerve...
January 1, 2018: Toxicologic Pathology
https://www.readbyqxmd.com/read/29786575/spatial-organization-of-the-excretory-ducts-and-sections-of-microcirculatory-blood-flow-of-the-labial-salivary-glands-in-older-adults
#3
Volodymyr H Hryn, Tamara F Deineha, Аndrij V Piliuhin, Anna V Lavrenko, Dmytro O Lavrenko
OBJECTIVE: Introduction: Salivary glands have a significant impact on the state of the homeostasis of the human body, oral cavity in particular, sensitively responding to pathological processes. The reactivity of the salivary glands in response to pathological processes that are organically linked to morphology and functions of the organ's structures, and particularly the excretory ducts of the glands and their microcirculatory blood flow, is one of the problems which have not been solved to date...
2018: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29783080/development-and-characterization-of-a-field-effect-biosensor-for-the-detection-of-acetoin
#4
Denise Molinnus, Lukas Muschallik, Laura Osorio Gonzalez, Johannes Bongaerts, Torsten Wagner, Thorsten Selmer, Petra Siegert, Michael Keusgen, Michael J Schöning
A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO2 /Ta2 O5 /enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T , has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied...
May 18, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29759237/evaluation-of-the-activity-of-%C3%AE-glucosidase-immobilized-on-polydimethylsiloxane-pdms-with-a-microfluidic-flow-injection-analyzer-with-embedded-optical-fibers
#5
Francisco M Hernández-Maya, María P Cañizares-Macías
β-glucosidase from almonds was immobilized on a polydimethylsiloxane (PDMS) microdevice by covalent chain using 3-aminopropyltrietoxysilane and glutaraldehyde. Enzymatic activity was evaluated using p-nitro-phenyl-β-D-glucopyranoside dissolved in a 0.01 M pH 5.0 phosphate solution at 45 °C measuring the reaction product (p-nitrophenol) at 410 nm. The microdevice consisted of two parts: the one part where the enzymatic reaction was carried out and a second part where pH was adjusted at 10, with NaOH...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759201/specific-detection-of-stable-single-nucleobase-mismatch-using-su-8-coated-silicon-nanowires-platform
#6
Melania Banu, Monica Simion, Marian C Popescu, Pericle Varasteanu, Mihaela Kusko, Ileana C Farcasanu
Novel microarray platform for single nucleotide polymorphisms (SNPs) detection has been developed using silicon nanowires (SiNWs) as support and two different surface modification methods for attaining the necessary functional groups. Accordingly, we compared the detection specificity and stability over time of the probes printed on SiNWs modified with (3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde (GAD), or coated with a simpler procedure using epoxy-based SU-8 photoresist. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were used for comparative characterization of the unmodified and coated SiNWs...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29749561/use-of-carbon-steel-ball-bearings-to-determine-the-effect-of-biocides-and-corrosion-inhibitors-on-microbiologically-influenced-corrosion-under-flow-conditions
#7
Tijan Pinnock, Johanna Voordouw, Gerrit Voordouw
A consortium of sulfate-reducing bacteria consisting mostly of Desulfovibrio, Desulfomicrobium, and Desulfocurvus from oil field produced water was cultivated in a chemostat, receiving medium with 20 mM formate and 10 mM sulfate as the energy and 1 mM acetate as the carbon source. The chemostat effluent, containing 5 mM sulfide and 0.5 mM of residual acetate, was passed through 1-ml syringe columns with 60 carbon steel ball bearings (BBs) of 53.6 ± 0.1 mg each at a flow rate of 0.8 ml/h per column...
May 10, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29746590/structural-and-immunologic-correlates-of-chemically-stabilized-hiv-1-envelope-glycoproteins
#8
Torben Schiffner, Jesper Pallesen, Rebecca A Russell, Jonathan Dodd, Natalia de Val, Celia C LaBranche, David Montefiori, Georgia D Tomaras, Xiaoying Shen, Scarlett L Harris, Amin E Moghaddam, Oleksandr Kalyuzhniy, Rogier W Sanders, Laura E McCoy, John P Moore, Andrew B Ward, Quentin J Sattentau
Inducing broad spectrum neutralizing antibodies against challenging pathogens such as HIV-1 is a major vaccine design goal, but may be hindered by conformational instability within viral envelope glycoproteins (Env). Chemical cross-linking is widely used for vaccine antigen stabilization, but how this process affects structure, antigenicity and immunogenicity is poorly understood and its use remains entirely empirical. We have solved the first cryo-EM structure of a cross-linked vaccine antigen. The 4.2 Å structure of HIV-1 BG505 SOSIP soluble recombinant Env in complex with a CD4 binding site-specific broadly neutralizing antibody (bNAb) Fab fragment reveals how cross-linking affects key properties of the trimer...
May 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29742352/bioconversion-of-lignocellulosic-biomass-to-fermentable-sugars-by-immobilized-magnetic-cellulolytic-enzyme-cocktails
#9
Karthik Periyasamy, Laishram Santhalembi, Gerard Mortha, Marc Aurousseau, Agnes Boyer, Sivanesan Subramanian
Enzyme cocktails of reusable, highly-stable cellulolytic enzymes play an inevitable role in bioconversion of biomass to biofuels economically. Cellulase, xylanase and β-1,3-glucanase bound silica-amine functionalized iron oxide magnetic nanoparticles (ISN-CLEAs) were prepared and used as the biocatalyst for the depolymerization of cellulosic biomass into monomeric sugar in the present study. The Fe3 O4 -NPs and Fe3 O4 @SiO2 -NH2 -NPs and ISN-CLEAs had an average hydrodynamic size of 82.2 nm, 86.4 nm and 976...
May 9, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29733931/a-novel-step-towards-immobilization-of-biocatalyst-using-agro-waste-and-its-application-for-ester-synthesis
#10
Prerana D Tomke, Virendra K Rathod
This work explains the utilization of agro-waste (coconut and peanut shell) to produce mesoporous activated carbon which further utilized as a support material for lipase immobilization (Candida antarctica B, CALB). Various parameters affecting the binding of enzyme to activated carbon with high surface area (1603 m2  g-1 ) were optimized. Maximum 200 μg g-1 CALB has been loaded at 40 °C and pH 6.8 in 12 h by using glutaraldehyde as a cross-linker. The operational parameters such as pH (5.8-8...
May 4, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29723772/construction-of-conducting-polymer-cytochrome-c-thylakoid-membrane-based-photo-bioelectrochemical-fuel-cells-generating-high-photocurrent-via-photosynthesis
#11
Emre Cevik, Buket Bezgin Carbas, Mehmet Senel, Huseyin Bekir Yildiz
In this study, a photo-bioelectrochemical fuel cell was constructed for photocurrent generation by illuminating the electrodes within an aqueous solution. In this purpose, gold electrode was coated with poly 4-(4H-Dithieno [3,2-b:2',3'-d]pyrol-4-yl) aniline, P(DTP-Ph-NH2 ) conductive polymer film by using electrochemical polymerization. Then, P(DTP-Ph-NH2 ) conductive polymer film coated surface was electrochemically modified with cytochrome C which covalently linked onto the surface via bis-aniline functionality of the polymer film and formed crosslinked-structure...
April 26, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29719697/fabrication-of-microstructured-binary-polymer-brush-corrals-with-integral-ph-sensing-for-studies-of-proton-transport-in-model-membrane-systems
#12
J Madsen, R E Ducker, O Al Jaf, M L Cartron, A M Alswieleh, C H Smith, C N Hunter, S P Armes, G J Leggett
Binary brush structures consisting of poly(cysteine methacrylate) (PCysMA) "corrals" enclosed within poly(oligoethylene glycol methyl ether methacrylate) (POEGMA) "walls" are fabricated simply and efficiently using a two-step photochemical process. First, the C-Cl bonds of 4-(chloromethyl)phenylsilane monolayers are selectively converted into carboxylic acid groups by patterned exposure to UV light through a mask and POEGMA is grown from unmodified chlorinated regions by surface-initiated atom-transfer radical polymerisation (ATRP)...
February 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29709828/effect-of-ph-on-the-adsorption-and-interactions-of-bovine-serum-albumin-with-functionalized-silicon-nitride-surface
#13
Claudio Victor Dos Santos Junior, Marcia Soares Sader, Gabriel Cardoso Gonçalves, Gilberto Weissmüller, Renata Antoun Simão
Force-distance curves between atomic force microscopy (AFM) tip (Si3 N4 non-functionalized) and bovine serum Albumin (BSA) immobilized on Si3 N4 substrates have been performed with the purpose to understand how multiple interactions between the protein and the tip were favored in different pHs (4, 6 and 10). In this work, 100 silicon wafer samples were used to deposit a layer of Si3 N4 . Protein immobilization consisted of the silanization of the substrates with 3-aminopropyltriethoxysilane (APTES) and crosslinking with glutaraldehyde (GA)...
March 29, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29709778/electrochemical-immunosensor-for-differential-diagnostic-of-wuchereria-bancrofti-using-a-synthetic-peptide
#14
Isis C Prado, Verônica G Mendes, André L A Souza, Rosa F Dutra, Salvatore G De-Simone
Lymphatic filariasis (LF) is a neglected tropical disease transmitted by mosquitoes and the second cause of permanent disability leading to a significant morbidity and mortality rate. Previously, we have identified epitopes of the filarial abundant larval transcript-2 (ALT-2) protein using a microarray mapping. In this study, one of the epitopes (Wb/ALT2-A5) was used to construct an electrochemical immunosensor. Electrochemical technique of cyclic voltammetry was performed for detecting the signal generated by the interaction between the (Wb/ALT2-A5) peptide and circulating antibodies of serum human samples...
April 22, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29709537/anti-thrombogenicity-and-permeability-of-polyethersulfone-hollow-fiber-membrane-with-sulfonated-alginate-toward-blood-purification
#15
Esmaeil Salimi, Azadeh Ghaee, Ahmad Fauzi Ismail, Majid Karimi
The main aim of this study was to evaluate the suitability of sulfonated alginate as a modifying agent to enhance the hemocompatibility of self-fabricated polyethersulfone (PES) hollow fiber membrane for blood detoxification. Sodium alginate was sulfonated with a degree of 0.6 and immobilized on the membrane via surface amination and using glutaraldehyde as cross-linking agent. Coating layer not only improved the membrane surface hydrophilicity, but also induced -39.2 mV negative charges on the surface. Water permeability of the modified membrane was enhanced from 67 to 95 L/m2 ·h·bar and flux recovery ratio increased more than 2-fold...
April 27, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29695259/porcine-pulmonary-valve-decellularization-with-naoh-based-vs-detergent-process-preliminary-in-vitro-and-in-vivo-assessments
#16
Mathieu van Steenberghe, Thomas Schubert, Sébastien Gerelli, Caroline Bouzin, Yves Guiot, Daela Xhema, Xavier Bollen, Karim Abdelhamid, Pierre Gianello
BACKGROUND: Glutaraldehyde fixed xenogeneic heart valve prosthesis are hindered by calcification and lack of growth potential. The aim of tissue decellularization is to remove tissue antigenicity, avoiding the use of glutaraldehyde and improve valve integration with low inflammation and host cell recolonization. In this preliminary study, we investigated the efficacy of a NaOH-based process for decellularization and biocompatibility improvement of porcine pulmonary heart valves in comparison to a detergent-based process (SDS-SDC0, 5%)...
April 25, 2018: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/29693317/1-3-regiospecific-ethanolysis-of-soybean-oil-catalyzed-by-crosslinked-porcine-pancreas-lipase-aggregates
#17
Margarita D Ramos, Letícia P Miranda, Raquel L C Giordano, Roberto Fernandez-Lafuente, William Kopp, Paulo W Tardioli
The preparation of crosslinked aggregates of pancreatic porcine lipase (PPL-CLEA) was systematically studied, evaluating the influence of three precipitants and two crosslinking agents, as well as the use of soy protein as an alternative feeder protein on the catalytic properties and stability of the immobilized PPL. Standard CLEAs showed a global yield (CLEA' observed activity/offered total activity) of less than 4%, whereas with the addition of soy protein (PPL:soy protein mass ratio of 1:3) the global yield was approximately 5-fold higher...
April 25, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29691009/3d-macroporous-chitosan-based-scaffolds-with-in-situ-formed-pd-and-pt-nanoparticles-for-nitrophenol-reduction
#18
Dmitriy Berillo, Andrew Cundy
3D-macroporous chitosan-based scaffolds (cryogels) were produced via growth of metal-polymer coordinated complexes and electrostatic interactions between oppositely charged groups of chitosan and metal ions under subzero temperatures. A mechanism of reduction of noble metal complexes inside the cryogel walls by glutaraldehyde is proposed, which produces discrete and dispersed noble metal nanoparticles. 3D-macroporous scaffolds prepared under different conditions were characterised using TGA, FTIR, nitrogen adsorption, SEM, EDX and TEM, and the distribution of platinum nanoparticles (PtNPs) and palladium nanoparticles (PdNPs) in the material assessed...
July 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29683652/impacts-of-glutaraldehyde-on-microbial-community-structure-and-degradation-potential-in-streams-impacted-by-hydraulic-fracturing
#19
Maria Fernanda Campa, Stephen M Techtmann, Caleb M Gibson, Xiaojuan Zhu, Megan Patterson, Amanda Garcia de Matos Amaral, Nikea Ulrich, Shawn R Campagna, Christopher J Grant, Regina Lamendella, Terry C Hazen
The environmental impacts of hydraulic fracturing, particularly those of surface spills in aquatic ecosystems, are not fully understood. The goals of this study were to (1) understand the effect of previous exposure to hydraulic fracturing fluids on aquatic microbial community structure and (2) examine the impacts exposure has on biodegradation potential of the biocide glutaraldehyde. Microcosms were constructed from hydraulic fracturing-impacted and nonhydraulic fracturing-impacted streamwater within the Marcellus shale region in Pennsylvania...
April 30, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29649903/pharmacokinetic-evaluation-of-quetiapine-fumarate-controlled-release-hybrid-hydrogel-a-healthier-treatment-of-schizophrenia
#20
Muhammad Akhlaq, Faiza Maryam, Abdelhamid Elaissari, Hashmat Ullah, Muhammad Adeel, Abid Hussain, Muhammad Ramzan, Obaid Ullah, Muhammad Zeeshan Danish, Shehla Iftikhar, Nighat Aziz
The current study aimed to rationally develop and characterize pH-sensitive controlled release hydrogels by graft polymerization of gelatin (Gel) and hydroxypropyl methyl cellulose (HPMC) in the presence of glutaraldehyde (GA) using quetiapine fumarate for the treatment of schizophrenia. The prepared hydrogels discs were subjected to various physicochemical studies including: swelling, diffusion, porosity, sol-gel analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and scanning electron microscopy...
November 2018: Drug Delivery
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