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Glutaraldehyde 2%

Naoto Fukunaga, Ryuzo Sakata, Tadaaki Koyama
OBJECTIVES: We reviewed reoperations following mitral valve repair (MVR) that used glutaraldehyde-treated autologous pericardium for mitral regurgitation (MR) to analyse the durability and risk factors for reoperation. METHODS: We retrospectively analysed 144 patients (mean age 57.9 years) who underwent MVR using glutaraldehyde-treated pericardium from March 1992 to December 2011. A total of 19 reoperations were necessary during the follow-up period (mean 6.9 years)...
June 21, 2017: Interactive Cardiovascular and Thoracic Surgery
Sina Stock, Hans-Hinrich Sievers, Doreen Richardt, Michael Scharfschwerdt
OBJECTIVES: : Small aortic annuli are challenging in aortic valve replacement. Prosthesis-patient mismatch, accompanied by high transvalvular gradients and small orifice area, is an often faced problem impairing postoperative outcome. The new TRIBIO bioprosthesis aims to enable enhanced haemodynamics, being increasingly important with decreasing annular size. This study compares ex vivo hydrodynamics of TRIBIO with 2 established bioprostheses in small annuli at different stroke volumes, simulating 'rest' and 'exercise', and evaluates haemodynamics of TRIBIO in a sheep model...
June 20, 2017: Interactive Cardiovascular and Thoracic Surgery
Nan Hao, Xuan Zhang, Zhou Zhou, Rong Hua, Ying Zhang, Qian Liu, Jing Qian, Henan Li, Kun Wang
It is necessary to develop rapid, simple and accurate detection method for Escherichia coli (E. coli) due to its widely distributed pathogenic bacteria. Herein, we prepared AgBr nanoparticles (NPs) anchored 3D nitrogen-doped graphene hydrogel (3DNGH) nanocomposites with an exceptionally large accessible surface by a simple hydrothermal approach. The as-prepared 3DNGH porous nanocomposite not only showed better conductivity than that of 3D graphene due to introducing nitrogen element into graphene framework, but also provided a high loading volume for immobilizing luminol...
June 13, 2017: Biosensors & Bioelectronics
Gabriella Sanzò, Irene Taurino, Francesca Puppo, Riccarda Antiochia, Lo Gorton, Gabriele Favero, Franco Mazzei, Sandro Carrara, Giovanni De Micheli
In this work, we have developed for the first time a method to make novel gold and platinum hybrid bimetallic nanostructures differing in shape and size. Au-Pt nanostructures were prepared by electrodeposition in two simple steps. The first step consists of the electrodeposition of nanocoral Au onto a gold substrate using hydrogen as a dynamic template in an ammonium chloride solution. After that, the Pt nanostructures were deposited onto the nanocoral Au organized in pores. Using Pt (II) and Pt (IV), we realized nanocoral Au decorated with Pt nanospheres and nanocoral Au decorated with Pt nanoflowers, respectively...
June 6, 2017: Methods: a Companion to Methods in Enzymology
Muhammad Zafir Mohamad Nasir, Carmen C Mayorga-Martinez, Zdeněk Sofer, Martin Pumera
Single or few layers lithium-exfoliated transition metal dichalcogenides (TMDs) are found to exist predominantly in the conducting metallic 1T-polymorph, which makes it desirable for numerous applications due to its large surface area, good electrical conductivity, and enhanced electrocatalytic capabilities. We demonstrated the use of tert-butyllithium exfoliated TMDs (MoS2, MoSe2, WS2, WSe2) as a platform for the indirect electrochemical detection of an organophosphate pesticide, fenitrothion, via enzymatic inhibition pathway...
June 6, 2017: ACS Nano
M Soledad Orellano, Carina Porporatto, Juana J Silber, R Darío Falcone, N Mariano Correa
It is known that Chitosan (Ch) can be used in several applications, such as antimicrobial agent or as drug delivery agent. However, being its water dispersibility very low at physiological pH it is necessary to find a way to improve it. One attractive strategy is to synthesize Chitosan Nanoparticles (Ch-NPs). In this work, a versatile method to obtain Ch-NPs with different and controlled sizes, that were successfully prepared by cross-linking reaction of glutaraldehyde and native chitosan inside of n-heptane/sodium 1,4-bis-2-ethylhexylsulfosuccinate (AOT)/water reverse micelles (RMs) is presented...
September 1, 2017: Carbohydrate Polymers
Zafar Ali, Lei Tian, Baoliang Zhang, Nisar Ali, Muhammad Khan, Qiuyu Zhang
The mesoporous yolk-shell nanoparticles (MYSNs), with a movable Fe3O4 core inside the hollow capsules, with two different morphologies (1) Fe3O4@SiO2@hollow@fibrous SiO2 (Yolk Shell-1) (2) Fe3O4@SiO2@hollow@mesoporousSiO2 (Yolk Shell-2) were fabricated as carriers for Candida rugosa lipase (CRL) immobilization, through a template-assistant selectively etching method. Firstly the hydrophilic Fe3O4 nanoparticles were synthesized according to the solvothermal method. Then, SiO2 shell was coated on the Fe3O4 nanoparticle surface by a sol-gel reaction...
August 2017: Enzyme and Microbial Technology
Asta Kausaite-Minkstimiene, Ruta Simanaityte, Almira Ramanaviciene, Laura Glumbokaite, Arunas Ramanavicius
A reagent-less amperometric glucose biosensor operating in not-stirred sample solution was developed. A working electrode of the designed biosensor was based on a graphite rod (GR) electrode, which was modified with 1,10-phenanthroline-5,6-dione (PD) and glucose oxidase (GOx). The PD and the GOx were layer-by-layer adsorbed on the GR electrode surface with subsequent drying followed by chemical cross-linking of the adsorbed GOx with glutaraldehyde (GA). Optimal preparation conditions of the working electrode (GR/PD/GOx) were achieved with 12...
August 15, 2017: Talanta
Michelle J Alfa, Maira M Ribeiro, Cristiana da Costa Luciano, Rodrigo Franca, Nancy Olson, Pat DeGagne, Harminder Singh
BACKGROUND AND AIMS: Clinical studies have shown variable culture results from flexible endoscope channels possibly due to low levels of bacteria that are difficult to extract. The aim of this study was to develop a simulated-use buildup biofilm (BBF) model that mimics low levels of viable bacteria after repeated rounds of aldehyde fixation and accumulation. METHODS: New endoscope channels were exposed to eight days of repeated rounds of biofilm formation using ATS2015 containing Enterococcus faecalis and Pseudomonas aeruginosa, rinsing, fixation with glutaraldehyde and rinsing...
May 24, 2017: Gastrointestinal Endoscopy
Markus Schlömicher, Zulfugar Taghiyev, Yazan Al-Jabery, Peter Lukas Haldenwang, Leif Markthaler, Vadim Moustafine, Axel Maria Laczkovics, Justus Thomas Strauch, Matthias Bechtel
BACKGROUND: The Labcor TBLP Supra™ prosthesis is a second-generation tissue valve that has evolved from the Labcor TBLP™ prosthesis, first introduced in 1984. The Supra prosthesis is a triple-composite porcine valve, with no specific anticalcification treatment being used in its production other than standard tissue fixation with 0.4% glutaraldehyde. To date, more than 100,000 Supra valves have been implanted, mainly in South America. Despite this remarkably high number of implants, only marginal data exist concerning durability and safety issues...
January 2017: Journal of Heart Valve Disease
E Suesca, A M A Dias, M E M Braga, H C de Sousa, M R Fontanilla
This work evaluates the effect of processing variables on some physicochemical and mechanical properties of multi- and unidirectional laminar collagen type I scaffolds. The processing variables considered in this study included microstructure orientation (uni- and multidirectional fiber/pore controlled by freeze-drying methodology), cross-linking (chemical - using genipin and glutaraldehyde, and physical - using a dehydrothermal method), and collagen concentration (2, 5 and 8mg/ml). The biocompatibility of the scaffolds obtained in each of the evaluated manufacturing processes was also assessed...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Mengdi Wu, Kun Feng, Qiuhui Li, Huiya Ma, Hongli Zhu, Yudou Xie, Gaofei Yan, Chao Chen, Kunping Yan
A Tempol compound with an amine group (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl, NH2-Tempol) was cross-linked to hemoglobin in a one-step polymerization reaction to produce a novel hemoglobin-based oxygen carrier (HBOC) designated PolyHb-Tempol. The reaction parameters, including the reaction time, pH, temperature, and ratio of reactants, were optimized, and the physiochemical properties of the resulting product were characterized. PolyHb-Tempol didn't show any toxicity towards endothelial cells. Furthermore, from observations of cell morphology and viability, PolyHb-Tempol showed a significant ability to inhibit or eliminate oxidative stress induced by superoxide free radicals...
May 18, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Chun Soo Park, Yong Jin Kim, Jeong Ryul Lee, Hong-Gook Lim, Ji-Eun Chang, Saeromi Jeong, Nayun Kwon
OBJECTIVES: : We aimed to investigate the effect of a combination of anticalcification treatments, which were effective for preventing calcification in a small animal experiment, on glutaraldehyde-fixed xenopericardial valves using a large-animal long-term circulatory model. METHODS: : Valved conduits were made of porcine pericardium as a leaflet and bovine pericardium as a conduit and were implanted into the right ventricular outflow tract of goats under cardiopulmonary bypass...
May 12, 2017: Interactive Cardiovascular and Thoracic Surgery
Tuğba Kotil, Cansu Akbulut, Nazan Deniz Yön
INTRODUCTION: Nanotechnology investigates materials at nanoscale level (0.1-100nm in diameter). There are many commercially nanoproducts such as silver, silicon, titanium, zinc, and gold. They are used in a variety of applications and released to the environment. Titanium dioxide (TiO2) is one of the most commonly used nanoparticles (NP). In this study, the ultrastructural effects of TiO2-NP on zebrafish testis tissue were evaluated. MATERIAL AND METHOD: Zebrafish were divided into four groups (N=60) as one control and 3 experimental groups (1mg/L, 2mg/L and 4mg/L TiO2)...
September 2017: Micron: the International Research and Review Journal for Microscopy
Jiwei Li, Yang Si, Cunyi Zhao, Jinmei He, Gang Sun, Yudong Huang
In this study, polyanionic alginate gel beads crosslinked by Ca(2+) and glutaraldehyde have demonstrated a strong electrostatic interaction with specific proteins. Due to the naturally abundant carboxyl groups, the prepared alginate gel beads exhibited a relatively superior integrated adsorption performance toward lysozyme, including a superior adsorption capacity of 213mgg(-1), fast adsorption equilibrium within 12h, good selectivity, and good reversibility. Compared with other protein adsorbents, the as-prepared adsorptive beads have the advantages of excellent adsorption performance, easy to prepare, convenient, efficient, reliable and environment-friendly to apply, which can serve as a more sustainable material in protein separation and purification...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Gisane Gasparotto, João Paulo C Costa, Paulo I Costa, Maria A Zaghete, Talita Mazon
In this research, ZnO nanorods - Au nanoparticles nanohybrids have been fabricated and employed to sensitive electrochemical strategy for the specific detection of the ovarian cancer antigen CA-125/MUC126. The microdevice was developed in our lab based on gold and silver electrodes sputtered on glass substrate. The ZnO nanorods arrays were grown on working electrode using assisted microwave hydrothermal synthesis than gold nanoparticles (Au NPs) were deposited by sputtering. The Au NPs onto ZnO nanorods surface provides a favorable platform for efficient loading of anti-CA-125 antibody via binding with cystamine and glutaraldehyde...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Xiaojian Li, Yaoguang Wang, Li Shi, Hongmin Ma, Yong Zhang, Bin Du, Dan Wu, Qin Wei
An electrochemiluminescence (ECL) biosensor was developed for detection of Concanavalin A (Con A). Chitosan/Ru(bpy)3(2+)/silica/Fe3O4 nanomaterials (CRuSi-Fe3O4) were synthesized through W/O microemulsion route. The added Fe3O4 nanoparticles can simplify the prepared process and enhance the conductivity of nanomaterials which can increase the ECL intensity of luminophor CRuSi-Fe3O4. In addition, the layered structure of CRuSi-Fe3O4 can immobilize lots of Con A using glutaraldehyde as the coupling agent which can improve the sensitivity of the biosensor...
May 1, 2017: Biosensors & Bioelectronics
Yuan Zhang, Dongdong Yu, Jiangbo Dai, Xu Wang, Zhongxin Zhu, Yewei Huang, Jia Sun, Zhiwei Chen, Litai Jin, Yang Wang
We report here a rapid and sensitive technique for negative visualization of protein in 1D and 2D SDS-PAGE by using 2', 7'-dichlorofluorescein (DCF), which appeared as transparent and colorless bands in an opaque gel matrix background. For DCF stain, down to 0.1-0.2 ng protein could be easily visualized within 7 min by only two steps, and the staining is fourfold more sensitive than that of Eosin Y (EY) negative stain and glutaraldehyde (GA) silver stain, and eightfold more sensitive than that of the commonly used imidazole-zinc (IZ) negative stain...
May 3, 2017: Proteomics
Muamer Dervisevic, Esma Dervisevic, Mehmet Senel, Emre Cevik, Huseyin Bekir Yildiz, Pınar Camurlu
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies...
July 2017: Enzyme and Microbial Technology
José C S Dos Santos, Horacio L Bonazza, Leonardo J B L de Matos, Elizabete A Carneiro, Oveimar Barbosa, Roberto Fernandez-Lafuente, Luciana R B Gonçalves, Hosiberto B de Sant' Ana, Rílvia S Santiago-Aguiar
The objective of this new paper was to evaluate the enzymatic esterification reaction conducted in supercritical or near-critical CO2, catalyzed by immobilized lipase B from Candida antarctica (CALB). The biocatalyst was prepared through the immobilization of CALB by covalent attachment using chitosan sequentially activated with Glycidol, ethylenediamine (EDA) and glutaraldehyde as support. In order to determine the best operational conditions of the esterification reaction (1: 1 (alcohol-acid); biocatalyst content, 10% (by substrate mass); 45 °C), an experimental design (2(3)) was conducted to evaluate the effects of the following parameters: alcohol to oil molar ratios, reaction time and temperature...
March 2017: Biotechnology Reports
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