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https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#1
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28881302/nanoparticulation-of-bovine-serum-albumin-and-poly-d-lysine-through-complex-coacervation-and-encapsulation-of-curcumin
#2
L Maldonado, R Sadeghi, J Kokini
Soluble coacervate nanoparticles were fabricated by mixing bovine serum albumin (BSA) and poly-d-lysine with low (LMW-PDL) and high molecular weights (HMW-PDL). The particle size was influenced by molecular weight, mass ratio of polyelectrolytes (PEs), and salt concentration. The smallest nanoparticles had a diameter of 212±11nm which was achieved with LMW-PDL dissolved with 0.1M NaCl at pH 7 and a mass ratio of 2.0 (BSA: PDL). SEM images showed that coacervate nanoparticles of LMW-PDL are relatively spherical in shape, while nanoparticles of HMW-PDL were irregular...
August 30, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28869533/construction-of-a-fluorescent-h%C3%A2-o%C3%A2-biosensor-with-chitosan-6-oh-immobilized-%C3%AE-cyclodextrin-derivatives
#3
Wenbo Dong, Weiping Li, Yu Chen, Yanchun Ye, Shaohua Jin
In the present work, a fluorescent H₂O₂ biosensor was constructed by encapsulating fluorescent probe Rhodamine B (RhmB) in the hydrophobic cavity of the cyclodextrin (β-CD) and immobilizing catalase (CAT) on the 2-NH₂ of chitosan (CTS) in a chitosan 6-OH immobilized β-cyclodextrin derivative (CTS-6-CD). The inclusion complex of CTS-6-CD to RhmB (CTS-6-CD-RhmB) was prepared by a solution method. Its structure and inclusion efficiency were determined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and fluorescence spectroscopy (FL)...
September 4, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28869529/different-covalent-immobilizations-modulate-lipase-activities-of-hypocrea-pseudokoningii
#4
Marita G Pereira, Susana Velasco-Lozano, Sonia Moreno-Perez, Aline M Polizeli, Paulo R Heinen, Fernanda D A Facchini, Ana C Vici, Mariana Cereia, Benevides C Pessela, Gloria Fernandez-Lorente, Jose M Guisan, João A Jorge, Maria de Lourdes T M Polizeli
Enzyme immobilization can promote several advantages for their industrial application. In this work, a lipase from Hypocrea pseudokoningii was efficiently linked to four chemical supports: agarose activated with cyanogen bromide (CNBr), glyoxyl-agarose (GX), MANAE-agarose activated with glutaraldehyde (GA) and GA-crosslinked with glutaraldehyde. Results showed a more stable lipase with both the GA-crosslinked and GA derivatives, compared to the control (CNBr), at 50 °C, 60 °C and 70 °C. Moreover, all derivatives were stabilized when incubated with organic solvents at 50%, such as ethanol, methanol, n-propanol and cyclohexane...
September 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28865360/quantification-of-cell-substratum-interactions-by-atomic-force-microscopy
#5
Qian Li, Thomas Becker, Wolfgang Sand
Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2...
August 30, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28859802/combination-of-physico-chemical-entrapment-and-crosslinking-of-low-activity-laccase-based-biocathode-on-carboxylated-carbon-nanotube-for-increasing-biofuel-cell-performance
#6
Marcelinus Christwardana
New laccase-based catalysts to improve oxygen reduction reactions (ORR) are described, and enzymatic biofuel cells (EBCs) adopting these catalysts were developed. These new catalysts are synthesized by combining laccase, poly(ethylenimine) and carbon nanotubes, with attachment of selected elements using the crosslinker, glutaraldehyde (GA). Several characterization approaches are implemented to evaluate catalytic electron transfer in both the absence and presence of mediators and their effects on glucose/O2 biofuel cell performance...
November 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28854757/cleaning-and-disinfection-programs-against-campylobacter-jejuni-for-broiler-chickens-productive-performance-microbiological-assessment-and-characterization
#7
Maria Fernanda de Castro Burbarelli, Gustavo do Valle Polycarpo, Karoline Deliberali Lelis, Carlos Alexandre Granghelli, Agatha Cristina Carão de Pinho, Sabrina Ribeiro Almeida Queiroz, Andrezza Maria Fernandes, Ricardo Luiz Moro de Souza, Maria Estela Gaglianone Moro, Roberto de Andrade Bordin, Ricardo de Albuquerque
Detailed cleaning and disinfection programs aims to reduce infection pressure from microorganisms from one flock to the next. However, studies evaluating the benefits to poultry performance, the sanitary status of the facilities, and the sanitary quality of the meat are rarely found. Thus, this study was designed to evaluate 2 cleaning and disinfecting programs regarding their influence on productive performance, elimination of Campylobacter, and characterization of Campylobacter jejuni strains when applied to broiler chickens' facilities...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28841980/electrochemical-enzyme-biosensors-based-on-calcium-phosphate-materials-for-tyramine-detection-in-food-samples
#8
Marta Sánchez-Paniagua López, Esther Redondo-Gómez, Beatriz López-Ruiz
Electrochemical tyrosinase biosensors for tyramine determination were developed by the immobilization of the enzyme in calcium phosphate materials (CaPs) followed by cross-linking with glutaraldehyde. Tyramine was detected by the electrochemical reduction at -0.1V of the o- enzymatically-formed dopaquinone. Three different CaPs were explored as immobilization systems, monetite, brushite and brushite cement. Biosensors based on brushite matrices provide better analytical properties than the monetite one. Compared to brushite, a 10-fold increase of sensitivity was obtained with the brushite cement-based biosensor, which highlights the effect of brushite crystal formation in the presence of the enzyme in the biosensor performance...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28827183/improved-tumor-tissue-penetration-and-tumor-cell-uptake-achieved-by-delayed-charge-reversal-nanoparticles
#9
Jingxin Gou, Yuheng Liang, Linlin Miao, Wei Guo, Yanhui Chao, Haibing He, Yu Zhang, Jingyu Yang, Chunfu Wu, Tian Yin, Yanjiao Wang, Xing Tang
The high affinity of positively charged nanoparticles to biological interfaces makes them easily taken up by tumor cells but limits their tumor permeation due to non-specific electrostatic interactions. In this study, polyion complex coated nanoparticles with different charge reversal profiles were developed to study the influence of charge reversal profile on tumor penetration. The system was constructed by polyion complex coating using micelles composed of poly (lysine)-b-polycaprolactone (PLys-b-PCL) as the cationic core and poly (glutamic acid)-g- methoxyl poly (ethylene glycol) (PGlu-g-mPEG) as the anionic coating material...
August 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28820469/a-third-generation-glucose-biosensor-based-on-cellobiose-dehydrogenase-immobilized-on-a-glassy-carbon-electrode-decorated-with-electrodeposited-gold-nanoparticles-characterization-and-application-in-human-saliva
#10
Paolo Bollella, Lo Gorton, Roland Ludwig, Riccarda Antiochia
Efficient direct electron transfer (DET) between a cellobiose dehydrogenase mutant from Corynascus thermophilus (CtCDH C291Y) and a novel glassy carbon (GC)-modified electrode, obtained by direct electrodeposition of gold nanoparticles (AuNPs) was realized. The electrode was further modified with a mixed self-assembled monolayer of 4-aminothiophenol (4-APh) and 4-mercaptobenzoic acid (4-MBA), by using glutaraldehyde (GA) as cross-linking agent. The CtCDH C291Y/GA/4-APh,4-MBA/AuNPs/GC platform showed an apparent heterogeneous electron transfer rate constant (ks) of 19...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28780853/inhibition-of-biodegradation-of-hydraulic-fracturing-compounds-by-glutaraldehyde-groundwater-column-and-microcosm-experiments
#11
Jessica D Rogers, Imma Ferrer, Shantal S Tummings, Angela R Bielefeldt, Joseph N Ryan
The rapid expansion of unconventional oil and gas development has raised concerns about the potential contamination of aquifers; however, the groundwater fate and transport of hydraulic fracturing fluid compounds and mixtures remains a significant data gap. Degradation kinetics of five hydraulic fracturing compounds (2-propanol, ethylene glycol, propargyl alcohol, 2-butoxyethanol, and 2-ethylhexanol) in the absence and presence of the biocide glutaraldehyde were investigated under a range of redox conditions using sediment-groundwater microcosms and flow-through columns...
August 25, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28765670/microleakage-in-class-v-composite-restorations-after-desensitizing-surface-treatment-with-er-yag-and-co2-lasers
#12
Hamid Reza Mozaffari, Alireza Ehteshami, Farshad Zallaghi, Nasim Chiniforush, Zohreh Moradi
Aims: Glutaraldehyde, CO2 and Er:YAG lasers can be used for treatment of dentin hypersensitivity. However, their application may have adverse effects on the clinical service of restorations. This study aimed to assess the microleakage in composite restorations following surface treatment with Glutaraldehyde desensitizer, CO2 and Er:YAG laser irradiation for treatment of dentin hypersensitivity. Materials and methods: This experimental study was conducted on 60 extracted sound human teeth. Class V cavities were prepared measuring 3×3 mm using a diamond bur...
December 30, 2016: Laser Therapy
https://www.readbyqxmd.com/read/28764006/potential-antioxidant-peptides-produced-from-whey-hydrolysis-with-an-immobilized-aspartic-protease-from-salpichroa-origanifolia-fruits
#13
Gabriela Fernanda Rocha, Francisco Kise, Adriana Mabel Rosso, Mónica Graciela Parisi
An aspartic protease from Salpichroa origanifolia fruits was successfully immobilized onto an activated support of glutaraldehyde agarose. The immobilized enzyme presented higher thermal stability than the free enzyme from 40°C to 50°C and high reusability, retaining 54% of the initial activity after ten cycles of the process. Whey protein concentrates (WPC) were hydrolyzed with both free and immobilized enzyme, reaching a similar degree of hydrolysis of approximately 6-8% after 20h. In addition, the immobilized derivate hydrolyzed α-lactalbumin protein with a higher affinity than β-lactoglobulin...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28762007/optimization-of-enzyme-co-immobilization-with-sodium-alginate-and-glutaraldehyde-activated-chitosan-beads
#14
Sinem Diken Gür, Neslihan İdil, Nilüfer Aksöz
In this study, two different materials-alginate and glutaraldehyde-activated chitosan beads-were used for the co-immobilization of α-amylase, protease, and pectinase. Firstly, optimization of multienzyme immobilization with Na alginate beads was carried out. Optimum Na alginate and CaCl2 concentration were found to be 2.5% and 0.1 M, respectively, and optimal enzyme loading ratio was determined as 2:1:0.02 for pectinase, protease, and α-amylase, respectively. Next, the immobilization of multiple enzymes on glutaraldehyde-activated chitosan beads was optimized (3% chitosan concentration, 0...
July 31, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28758327/characterization-of-pva-glutaraldehyde-hydrogels-obtained-using-central-composite-rotatable-design-ccrd
#15
Andreia de Araújo Morandim-Giannetti, Samantha Regina Rubio, Regina Freitas Nogueira, Fernando Dos Santos Ortega, Octaviano Magalhães Junior, Paulo Schor, Patrícia Alessandra Bersanetti
Hydrogels are made from natural or synthetic polymers and, currently, they have many biomedical applications. In this work, the conditions for obtaining a hydrogel with similar physicochemical characteristics to the vitreous humor were defined using polyvinyl alcohol (PVA) and glutaraldehyde (GLUT) as cross-linker. The concentration of PVA and GLUT were modified, and their effect was analyzed in terms of the refractive index, density, and dynamic viscosity. The hydrogel which was obtained using 3.98% (w/V) of PVA, 3...
July 31, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28757423/solid-solid-crosslinking-of-carboxymethyl-cellulose-nanolayer-on-titanium-oxide-nanoparticles-as-a-novel-biocomposite-for-efficient-removal-of-toxic-heavy-metals-from-water
#16
Mohamed E Mahmoud, Azza E H Abdou, Mostafa E Sobhy, Nesma A Fekry
Nano titanium oxide (Nano-TiO2) was used in this work as a solid support to solid-solid immobilize carboxymethyl cellulose via glutaraldehyde as an efficient cross linking reagent to produce a novel nanosized biocomposite (Nano-TiO2-glu-CMC). The synthesized materials were characterized by FT-IR to confirm crosslinking reaction between the two species as well as by HR-TEM, SEM, TGA and surface area analysis. The particle size was found to correspond to 22.2-44.4nm for Nano-TiO2-glu-CMC. The designed nanosized biocomposite was used and compared with Nano-TiO2 for separation and extraction of heavy metal ions such as Cd(II), Pb(II) and Hg(II) from aqueous solution by using batch experiment and microcolumn technique...
July 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28732732/porous-chitosan-beads-of-superior-mechanical-properties-for-the-covalent-immobilization-of-enzymes
#17
Marwa I Wahba
Porous chitosan beads of superior mechanical properties were produced via a two stepped treatment process. First, the chitosan ionotropic gelation solution was supplemented with Na2CO3, which acted as a porogen. Afterwards, the beads were chemically cross-linked with glutaraldehyde. This treatment also caused the produced porous chitosan beads to acquire higher observed activities of immobilized β-d-galactosidase (β-gal). The observed activities of the β-gal immobilized onto the 0.2M and the 0.35M Na2CO3 treated beads were 1...
July 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28728113/catalase-based-hydrogen-peroxide-biosensor-for-mercury-determination-by-inhibition-measurements
#18
Basant Elsebai, Mariana Emilia Ghica, Mohammed Nooredeen Abbas, Christopher M A Brett
A new amperometric hydrogen peroxide enzyme inhibition biosensor for the indirect determination of toxic mercury ions, Hg(2+), based on catalase immobilized on a glassy carbon electrode surface by cross-linking with glutaraldehyde and bovine serum albumin, is reported. The parameters influencing biosensor performance were optimized, including enzyme loading, the amount of hydrogen peroxide, the applied potential and electrolyte pH. It was shown that the inhibition of catalase by Hg(2+) species is irreversible, with a linear inhibition response between 5×10(-11) and 5×10(-10)M...
July 11, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28690263/direct-electron-transfer-type-bioelectrocatalysis-of-peroxidase-at-mesoporous-carbon-electrodes-and-its-application-for-glucose-determination-based-on-bienzyme-system
#19
Hong-Qi Xia, Yuki Kitazumi, Osamu Shirai, Kenji Kano
Non-catalytic direct electron transfer (DET) signal of Compound I of horseradish peroxidase (POD) was first detected at 0.7 V on POD/carbon nanotube mixture-modified electrodes. Excellent performance of DET-type bioelectrocatalysis was achieved with POD immobilized with glutaraldehyde on Ketjen Black (KB)-modified electrodes for H2O2 reduction with an onset potential of 0.65 V (vs. Ag | AgCl | sat. KCl) without any electrode surface modification. The POD-immobilized KB electrode was found to be suitable for detecting H2O2 with a low detection limit (0...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28686585/constructing-cellular-niche-properties-by-localized-presentation-of-wnt-proteins-on-synthetic-surfaces
#20
Molly Lowndes, Sergi Junyent, Shukry J Habib
Wnt signaling is crucial during embryonic development and for the maintenance of adult tissues. Depending on the tissue type, the Wnt pathway can promote stem cell self-renewal and/or direct lineage commitment. Wnt proteins are subject to lipid modification, often restricting them to act in a localized manner on responsive cells. Most methods for inducing Wnt signaling in stem cell cultures do not control the spatial presentation of the protein. To recreate the local presentation of Wnt proteins often seen in vivo, we previously developed a method to immobilize the protein onto synthetic surfaces...
July 2017: Nature Protocols
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