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https://www.readbyqxmd.com/read/29350201/moth-eye-mimetic-cytocompatible-bactericidal-nanotopography-a-convergent-design
#1
Felipe Viela, Iván Navarro-Baena, Jaime J Hernández, Manuel R Osorio, Isabel Rodriguez
The rapid emergence of antibiotic resistant bacteria has prompted the need for radically different approaches to combat bacterial infections. Among these, bioinspired surface topographies have emerged as an effective sustainable strategy to deter bacterial infection. This study demonstrates the bactericidal activity and cytocompatibility of the moth eye mimetic topography produced by thermal polymer nanoimprinting. The moth eye topography was found to have bactericidal capabilities against Gram negative and Gram positive bacteria...
January 19, 2018: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/29349550/recombinase-polymerase-amplification-combined-with-lateral-flow-dipstick-for-equipment-free-detection-of-salmonella-in-shellfish
#2
Weifang Gao, Hailong Huang, Peng Zhu, Xiaojun Yan, Jianzhong Fan, Jinpo Jiang, Jilin Xu
Salmonella is a major pathogen that causes acute foodborne outbreaks worldwide. Seafood, particularly shellfish, is a proven source of Salmonella spp. infection because many people prefer to eat it raw or lightly cooked. However, traditional identification methods are too time-consuming and complex to detect contamination of bacteria in the food chain in a timely manner, and few studies have aimed to identify Salmonella in shellfish early in the supply chain. We herein developed a method for rapid detection of Salmonella in shellfish based on the method of recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD), which targets the invasion gene A (invA)...
January 18, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29349489/characterization-of-multiple-antilisterial-peptides-produced-by-sakacin-p-producing-lactobacillus-sakei-subsp-sakei-2a
#3
Kátia G Carvalho, Felipe H S Bambirra, Jacques R Nicoli, Jamil S Oliveira, Alexandre M C Santos, Marcelo P Bemquerer, Antonio Miranda, Bernadette D G M Franco
Antimicrobial compounds produced by lactic acid bacteria can be explored as natural food biopreservatives. In a previous report, the main antimicrobial compounds produced by the Brazilian meat isolate Lactobacillus sakei subsp. sakei 2a, i.e., bacteriocin sakacin P and two ribosomal peptides (P2 and P3) active against Listeria monocytogenes, were described. In this study, we report the spectrum of activity, molecular mass, structural identity and mechanism of action of additional six antilisterial peptides produced by Lb...
January 18, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29347004/self-propulsion-against-a-moving-membrane-enhanced-accumulation-and-drag-force
#4
U Marini Bettolo Marconi, A Sarracino, C Maggi, A Puglisi
Self-propulsion (SP) is a main feature of active particles (AP), such as bacteria or biological micromotors, distinguishing them from passive colloids. A renowned consequence of SP is accumulation at static interfaces, even in the absence of hydrodynamic interactions. Here we address the role of SP in the interaction between AP and a moving semipermeable membrane. In particular, we implement a model of noninteracting AP in a channel crossed by a partially penetrable wall, moving at a constant velocity c. With respect to both the cases of passive colloids with c>0 and AP with c=0, the AP with finite c show enhancement of accumulation in front of the obstacle and experience a largely increased drag force...
September 2017: Physical Review. E
https://www.readbyqxmd.com/read/29345577/probiotics-and-paraprobiotics-in-viral-infection-clinical-application-and-effects-on-the-innate-and-acquired-immune-systems
#5
Osamu Kanauchi, Akira Andoh, Sazaly AbuBakar, Naoki Yamamoto
Recently, the risk of viral infection has dramatically increased owing to changes in human ecology such as global warming and an increased geographical movement of people and goods. However, the efficacy of vaccines and remedies for infectious diseases is limited by the high mutation rates of viruses, especially, RNA viruses. Here, we comprehensively review the effectiveness of several probiotics and paraprobiotics (sterilized probiotics) for the prevention or treatment of virally-induced infectious diseases...
January 16, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29339624/the-combined-effects-of-carbon-nitrogen-ratio-suspended-biomass-hydraulic-retention-time-and-dissolved-oxygen-on-nutrient-removal-in-a-laboratory-scale-anaerobic-anoxic-oxic-activated-sludge-biofilm-reactor
#6
D S Manu, Arun Kumar Thalla
The current trend in sustainable development deals mainly with environmental management. There is a need for economically affordable, advanced treatment methods for the proper treatment and management of domestic wastewater containing excess nutrients (such as nitrogen and phosphorus) which can cause eutrophication. The reduction of the excess nutrient content of wastewater by appropriate technology is of much concern to the environmentalist. In the current study, a novel integrated anaerobic-anoxic-oxic activated sludge biofilm (A2O-AS-biofilm) reactor was designed and operated to improve the biological nutrient removal by varying reactor operating conditions such as carbon to nitrogen (C/N) ratio, suspended biomass, hydraulic retention time (HRT) and dissolved oxygen (DO)...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29338194/sequential-and-selective-detection-of-two-molecules-with-a-single-solid-contact-chronopotentiometric-ion-selective-electrode
#7
Jiawang Ding, Nana Yu, Xuedong Wang, Wei Qin
A polymeric membrane ion-selective electrode (ISE) is typically designed for determination of one specific ion using conventional method. In this work, we demonstrate a simple, versatile and sensitive platform for simultaneous detection of two molecules with a single ISE. Under a series of periodic galvanostatic polarization, a solid-contact ISE without ion exchanger properties under zero-current conditions has been successfully used for simultaneous detection of two opposite charged ions with high sensitivity, good selectivity and fast reversibility...
January 17, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29335174/testing-association-between-soil-bacterial-diversity-and-soil-carbon-storage-on-the-loess-plateau
#8
Yang Yang, Yanxing Dou, Shaoshan An
Bacteria are widely distributed and play an important role in soil carbon (C) cycling. The impact of soil bacterial diversity on soil C storage has been well established, yet little is known about the underlying mechanisms and the interactions among them. Here, we examined the association between soil bacterial diversity and soil C storage in relation to vegetation restoration on the Loess Plateau. The dominant phyla among land use types (artificial forest, Af; natural shrubland, Ns; artificial grassland, Ag; natural grassland, Ng; slope cropland, Sc) were Acidobacteria, Actinobacteria, Alphaproteobacteria, and Betaproteobacteria, which transited from Acidobacteria-dominant to Actinobacteria-dominant community due to vegetation restoration...
January 12, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29334654/phbv-polymer-supported-denitrification-system-efficiently-treated-high-nitrate-concentration-wastewater-denitrification-performance-microbial-community-structure-evolution-and-key-denitrifying-bacteria
#9
Zhongshuo Xu, Liyan Song, Xiaohu Dai, Xiaoli Chai
Biodegradable polymer supported denitrification (BPD) system shows good denitrification performance for the wastewater with low nitrate concentrations. In this study, a BPD system using Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) polymer as carbon source was developed to treat the wastewater with high nitrate concentrations. The denitrification performance, utilization ratio of PHBV polymers, and microbial community structure evolution and key denitrifying bacteria were comprehensively studied. Results indicated that an average nitrate removal efficiency of 99% could be achieved with an influent NO3--N concentration of 100 mg L-1 and a hydraulic retention time (HRT) of 7...
January 8, 2018: Chemosphere
https://www.readbyqxmd.com/read/29333734/synthesis-cytotoxic-and-antibacterial-evaluation-of-quinazolinone-derivatives-with-substituted-amino-moiety
#10
Xiaoping Zhan, Yun Xu, Qi Qi, Yaolin Wang, Huiying Shi, Zhenmin Mao
A series of novel quinazolinone derivatives containing a substituted amino moiety were synthesized, evaluated for their cytotoxic and antibacterial activities. The results of MTT assay showed that all synthesized target compounds 5A~O showed potent cytotoxicity against SGC-7901 (IC50 , 0.72~1.41μM). Moreover, the compounds 5D, 5I and 5K showed better selectivity as compared with positive controls pemetrexed and MTX due to weak cytotoxicity against normal tissue cell line HUVSMC. Among synthesized compounds, the compounds 5E, 5J, 5L and 5N showed broad-spectrum cytotoxic activities against at least four cancer cell lines at a micromolar level...
January 14, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29331370/preparation-and-evaluation-of-antibacterial-potential-of-pithecellobium-dulce-root-extract-against-gram-positive-and-gram-negative-bacteria
#11
Muneer Ahmad Bhat, Rayees Ahmad Malik, Poonam Prakash, Ali Mohd Lone
In the present study hexane, benzene, ethyl acetate and ethanol extracts of Pithecellobium dulce root were prepared using soxhlet extractor. The extracts were evaluated for antibacterial activity against one Gram positive (Staphylococcus aureus) and three Gram negative (Acetobacter aceti, Acetobacter aceti, Klebsiella pneumoniae) strains. Disc diffusion method revealed promising antibacterial activity of the extracts prepared in polar solvents (ethyl acetate and ethanol) compared to non-polar solvents (hexane and benzene)...
January 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29327645/identification-and-characterization-of-chitinolytic-bacteria-isolated-from-a-freshwater-lake
#12
Dinh Minh Tran, Hayuki Sugimoto, Dzung Anh Nguyen, Takeshi Watanabe, Kazushi Suzuki
To develop a novel type of biocontrol agent, we focus on bacteria that are characterized by both chitinase activity and biofilm development. Chitinolytic bacteria were isolated from sediments and chitin flakes immersed in the water of a sand dune lake, Sakata, in Niigata, Japan. Thirty-one isolates from more than 5100 isolated strains were examined chitinase activity and biofilm formation. Phylogenetic analysis of these isolates based on the 16S rRNA gene sequences revealed that most isolates belonged to the family Aeromonadaceae, followed by Paenibacillaceae, Enterobacteriaceae, and Neisseriaceae...
January 12, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/29323625/catheter-associated-urinary-tract-infections-in-persons-with-neurogenic-bladders
#13
Todd A Linsenmeyer
This review is based on the author's Donald Munro Lecture given at the annual conference of the Academy of Spinal Cord Injury Professionals in New Orleans, LA. Indwelling catheters play an important role in bladder management following SCI for many individuals with neurogenic bladders. There is an increased risk of UTI compared to other types of bladder management with indwelling urethral catheters but not SP catheters. To minimize the risk of symptomatic UTI, the following steps are essential: prevent bladder wall distention and resulting ischemia, maintain colonization with "good" bacteria, and prevent bladder stones...
January 11, 2018: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/29323184/microbial-synthesis-of-a-useful-optically-active-isomer-of-lactone-with-bicyclo-4-3-0-nonane-structure
#14
Filip Boratyński, Agata Janik-Polanowicz, Ewa Szczepańska, Teresa Olejniczak
Lactone 2a of a bicyclo[4.3.0]nonane structure is a good starting material for synthesis of many attractive compounds. Enantiomerically enriched (-)-(3aR,7aS)-lactone 2a is produced by whole cells of bacteria. In order to examine the impact of the absolute configuration on biological activity we evaluated the process affording the opposite isomer. To this purpose Candida pelliculosa ZP22 characterized by high dehydrogenase activity was used. The goal of presented work was to perform bioreactor scale microbial one-pot oxidation of diol with selected yeast strain C...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321563/probiotic-activities-of-rhizobium-laguerreae-on-growth-and-quality-of-spinach
#15
Alejandro Jiménez-Gómez, José David Flores-Félix, Paula García-Fraile, Pedro F Mateos, Esther Menéndez, Encarna Velázquez, Raúl Rivas
The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29318288/green-one-pot-synthesis-of-novel-polysubstituted-pyrazole-derivatives-as-potential-antimicrobial-agents
#16
Hamid Beyzaei, Zahra Motraghi, Reza Aryan, Mohammad Mehdi Zahedi, Alireza Samzadeh-Kermani
Various biological properties of natural and synthetic pyrazole derivatives such as anti-inflammatory, antimicrobial, neuroprotective, anticonvulsant, antidepressant and anticancer activities encouraged us to propose a new, fast, green and eco-friendly procedure for the preparation of some novel 5-amino-3-(aryl substituted)-1-(2,4-dinitrophenyl)-1H-pyrazole-4-carbonitriles. They were efficiently synthesized via one-pot two-step process reaction of malononitrile, 2,4-dinitrophenylhydrazine and different benzaldehydes in deep eutectic solvent (DES) glycerol/potassium carbonate...
December 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/29317760/shape-dependent-physical-mutilation-and-lethal-effects-of-silver-nanoparticles-on-bacteria
#17
Debashish Acharya, K Malabika Singha, Piyush Pandey, Bidhan Mohanta, Jina Rajkumari, L Paikhomba Singha
In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentration (MIC) tests were conducted to study the antibacterial properties, and substantiated with killing kinetics of silver nanoparticles (AgNPs). The study revealed that both AgNP-sp and AgNRs are good antibacterial candidates...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315060/comparative-study-on-the-removal-efficiency-of-hydrogen-sulfide-h2s-using-three-different-packings
#18
Shihua Pu, Zuohua Liu, Feiyun Yang, Dingbiao Long, Wen Liu, Hao Wang, Kaipei Huang, Xiu Huang
To improve the removal efficiency on hydrogen sulfide (H2S), a biofilter was developed and was made of PVC pipes. The effects of three different packings (i.e., packing A, packing B, and packing C), containing different proportions of activated carbon, sawdust, wormcast, perlite, and pig manure compost, based on different biofilter parameters on H2S removal efficiency were investigated. With the extension of running time, the H2S removal rate of packing A reached up to 90.12%, that of packing B reached a peak at 92...
January 9, 2018: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/29314110/polyhydroxyalkanoates-pha-production-in-bacterial-co-culture-using-glucose-and-volatile-fatty-acids-as-carbon-source
#19
Sajida Munir, Nazia Jamil
Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used volatile fatty acids (VFAs) and glucose as carbon source to check the ability of selected strains ST2 (Pseudomonas sp.) and CS8 (Bacillus sp.) as co-culture. The production of PHA in pure co-cultures of bacteria was therefore investigated in order to understand the effect of combining cultures on PHA production parameters and material properties...
January 4, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29310458/evaluation-of-fus-2000-urine-analyzer-analytical-properties-and-particle-recognition
#20
Miroslava Beňovská, Ondřej Wiewiorka, Jana Pinkavová
This study evaluates the performance of microscopic part of a hybrid analyzer FUS-2000 (Dirui Industrial Co., Changchun, China), its analytical properties and particle recognition. The evaluation of trueness, repeatability, detection limit, carry-over, linearity range and analytical stability was performed according to Dirui protocol guidelines designed by Dirui Company to guarantee the quality of the instrument. Trueness for low, medium and high-value concentrations was calculated with bias of 15.5, 4.7 and -6...
January 8, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
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