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https://www.readbyqxmd.com/read/28721083/effect-of-a-lotion-containing-the-heat-treated-probiotic-strain-lactobacillus-johnsonii-ncc-533-on-staphylococcus-aureus-colonization-in-atopic-dermatitis
#1
Sandrine Blanchet-Réthoré, Valérie Bourdès, Annick Mercenier, Cyrille H Haddar, Paul O Verhoeven, Philippe Andres
OBJECTIVE: Staphylococcus aureus dominates the skin microbiota in patients with atopic dermatitis (AD), with bacterial loads correlating with disease severity. The aim of this exploratory study was to investigate the effect of a cosmetic lotion containing heat-treated Lactobacillus johnsonii NCC 533 (HT La1) on S. aureus colonization in AD patients. METHODS: This open-label, multicenter study was performed in AD patients in Germany. First, detection of S. aureus was performed in all patients using the swab or scrub-wash method of sampling, followed by quantitative culture or quantitative polymerase chain reaction...
2017: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/28721045/synthesis-construction-and-evaluation-of-self-assembled-nano-bacitracin-a-as-an-efficient-antibacterial-agent-in-vitro-and-in-vivo
#2
Wei Hong, Xiang Gao, Peng Qiu, Jie Yang, Mingxi Qiao, Hong Shi, Dexian Zhang, Chunlian Tian, Shengli Niu, Mingchun Liu
Bacitracin A (BA) is an excellent polypeptide antibiotic that is active against gram-positive bacteria without triggering multidrug resistance. However, BA is inactive against gram-negative bacteria because of its inability to cross the outer membrane of these cells, and it has strong nephrotoxicity, thus limiting its clinical applications. Nanoantibiotics can effectively localize antibiotics to the periplasmic space of bacteria while decreasing the adverse effects of antibiotics. In this study, biodegradable hydrophobic copolymers of poly (d,l-lactide-co-glycolide) (PLGA) were attached to the N-termini of BA to design a novel class of self-assembled nano-bacitracin A (nano-BAs), and their potential as antibacterial agents was evaluated in vitro and in vivo...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28720951/isolation-and-characterization-of-lactic-acid-bacteria-from-traditional-pickles-of-himachal-pradesh-india
#3
Monika, Savitri, Vijay Kumar, Anila Kumari, Kunzes Angmo, Tek Chand Bhalla
A total of 15 isolates of lactic acid bacteria (LAB) were isolated from traditional pickles of rural and urban areas of Himachal Pradesh. These isolates were identified as Enterococcus faecalis (7), Lactobacillus plantarum (3), Pediococcus pentosaceus (2), Leuconostoc mesenteroides (1), Lactococcus lactis (1) and Enterococcus sp. (1) on the basis of morphological, biochemical and 16S rDNA sequence analysis. All the LABs produced exopolysaccharides, have antibiotic susceptibility, and possess activity of β-galactosidase, β-glucosidase, protease and amylase, while none of the isolates showed haemolytic activity...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720941/chemical-composition-and-bioactivity-of-essential-oils-from-flower-and-fruit-of-thymbra-capitata-and-thymus-species
#4
Jonathan Delgado-Adámez, María Garrido, Maria Elena Bote, María C Fuentes-Pérez, Javier Espino, Daniel Martín-Vertedor
Thymbra capitata and Thymus species are commonly known as thyme in Spain and they are currently used as culinary herbs, as well as for ornamental, aromatizing and traditional medicinal purposes. Given the economic importance of thyme oils, many thyme species have been studied, and their essential oils (EOs) and other volatile-containing extracts have been chemically characterized. The aim of this study was to evaluate the antioxidant, antimicrobial and cytotoxic activities as well as the phytochemical composition of the EOs from fruits and flowers of T...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720731/an-in-vitro-chicken-gut-model-demonstrates-transfer-of-a-multidrug-resistance-plasmid-from-salmonella-to-commensal-escherichia-coli
#5
Roderick M Card, Shaun A Cawthraw, Javier Nunez-Garcia, Richard J Ellis, Gemma Kay, Mark J Pallen, Martin J Woodward, Muna F Anjum
The chicken gastrointestinal tract is richly populated by commensal bacteria that fulfill various beneficial roles for the host, including helping to resist colonization by pathogens. It can also facilitate the conjugative transfer of multidrug resistance (MDR) plasmids between commensal and pathogenic bacteria which is a significant public and animal health concern as it may affect our ability to treat bacterial infections. We used an in vitro chemostat system to approximate the chicken cecal microbiota, simulate colonization by an MDR Salmonella pathogen, and examine the dynamics of transfer of its MDR plasmid harboring several genes, including the extended-spectrum beta-lactamase blaCTX-M1 We also evaluated the impact of cefotaxime administration on plasmid transfer and microbial diversity...
July 18, 2017: MBio
https://www.readbyqxmd.com/read/28720301/a-quantitative-swab-is-a-good-non-invasive-alternative-to-a-quantitative-biopsy-for-quantifying-bacterial-load-in-wounds-healing-by-second-intention-in-horses
#6
L L Van Hecke, K Hermans, M Haspeslagh, K Chiers, E Pint, F Boyen, A M Martens
The aim of this study was to evaluate different techniques for diagnosing wound infection in wounds healing by second intention in horses and to assess the effect of a vortex and sonication protocol on quantitative bacteriology in specimens with a histologically confirmed biofilm. In 50 wounds healing by second intention, a clinical assessment, a quantitative swab, a semi-quantitative swab, and a swab for cytology were compared to a quantitative tissue biopsy (reference standard). Part of the biopsy specimen was examined histologically for evidence of a biofilm...
July 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28718982/design-and-solution-phase-synthesis-of-membrane-targeting-lipopeptides-with-selective-antibacterial-activity
#7
Mohini Mohan Konai, Utsarga Adhikary, Jayanta Haldar
Designing selective antibacterial molecules remains an unmet goal in the field of membrane targeting agents. To this aim, herein we report the rational design and synthesis of a new class of lipopeptides which possess highly selective bacterial killing over mammalian cells. The selective interaction with bacterial over mammalian membranes was established through various spectroscopic as well as microscopic experiments including biophysical studies with the model membranes. A detailed antibacterial structure-activity relationship was delineated after preparing a series of molecules consisting of the peptide moieties with varied sequence of amino acids such as D-phenylalanine, D-leucine and D-lysine...
July 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28718462/-preventive-dentistry-7-getting-rid-of-halitosis
#8
T M H de Jong, M L Laine
Halitosis or bad breath is a problem that affects many people and its source is generally found in the oral cavity (intra-oral halitosis). Bacteria in the oral cavity produce volatile sulphur compounds such as hydrogen sulphide and methyl mercaptan that not only emit an unpleasant odour, but may also have toxic effects on the periodontal tissues. Good oral hygiene, a healthy periodontium and healthy teeth are the basis for the prevention of intra-oral halitosis and dentists and dental hygienists therefore play an essential role in its prevention...
July 2017: Nederlands Tijdschrift Voor Tandheelkunde
https://www.readbyqxmd.com/read/28717295/animal-inflicted-maxillofacial-injuries-treatment-modalities-and-our-experience
#9
Anjani Kumar Yadav, Mehul Rajesh Jaisani, Leeza Pradhan, Ashok Dongol, Arpita Singh, Pradeep Acharya, Alok Sagtani
INTRODUCTION: Animal inflicted injuries to the face and neck are becoming much more common as people lavish affection on pets. Injuries caused by animal attacks to the face can cause complex injuries to soft and hard tissues, presented as perforations, lacerations, crushes, avulsion or fractures. An uncountable number of bacteria and virus can be found in such injuries, with a potential pathological effect to humans, regarding infections. Although the infection rate is low due to excellent blood supply to face, the injuries have disfiguring effect with possible psychological repercussion to the patients...
September 2017: Journal of Maxillofacial and Oral Surgery
https://www.readbyqxmd.com/read/28714250/development-of-antimicrobial-nanoemulsion-based-delivery-systems-against-selected-pathogenic-bacteria-using-a-thymol-rich-thymus-daenensis-essential-oil
#10
Lida Ghaderi, Roya Moghimi, Atousa Aliahmadi, David Julian McClements, Hasan Rafati
AIMS: Thymol rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water-solubility and high vapor pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in water by preventing Ostwald ripening and phase separation. METHODS & RESULTS: The antibacterial activity of bulk and emulsified essential oil against selected pathogenic bacteria including Gram-negative (Haemophilus influenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) were investigated in the liquid and vapor phase...
July 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28714045/characterization-drug-loading-and-antibacterial-activity-of-nanohydroxyapatite-polycaprolactone-nha-pcl-electrospun-membrane
#11
Mohd Izzat Hassan, Naznin Sultana
Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28713789/screening-of-oxalate-degrading-lactic-acid-bacteria-of-food-origin
#12
Nicoletta Murru, Giuseppe Blaiotta, Maria Francesca Peruzy, Serena Santonicola, Raffaelina Mercogliano, Maria Aponte
A screening for oxalate degrading abilities was initially carried on within Lactic Acid Bacteria cultures of different food origin. Seventy-nine strains were drop-inoculated onto MRS agar plates containing calcium oxalate. By comparing colonies diameters, 31 strains were used to inoculate, in parallel, MRS and MRS modified by sodium oxalate addition. Differences in the strains' growth were assessed by colony forming unit counts. For two strains, the growth in oxalate enriched medium was significantly higher; while, for eleven strains an opposite behaviour was recorded...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713356/a-genotypic-analysis-of-five-p-aeruginosa-strains-after-biofilm-infection-by-phages-targeting-different-cell-surface-receptors
#13
Diana P Pires, Andreas Dötsch, Erin M Anderson, Youai Hao, Cezar M Khursigara, Joseph S Lam, Sanna Sillankorva, Joana Azeredo
Antibiotic resistance constitutes one of the most serious threats to the global public health and urgently requires new and effective solutions. Bacteriophages are bacterial viruses increasingly recognized as being good alternatives to traditional antibiotic therapies. In this study, the efficacy of phages, targeting different cell receptors, against Pseudomonas aeruginosa PAO1 biofilm and planktonic cell cultures was evaluated over the course of 48 h. Although significant reductions in the number of viable cells were achieved for both cases, the high level of adaptability of the bacteria in response to the selective pressure caused by phage treatment resulted in the emergence of phage-resistant variants...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28713353/functional-characterization-of-novel-faecalibacterium-prausnitzii-strains-isolated-from-healthy-volunteers-a-step-forward-in-the-use-of-f-prausnitzii-as-a-next-generation-probiotic
#14
Rebeca Martín, Sylvie Miquel, Leandro Benevides, Chantal Bridonneau, Véronique Robert, Sylvie Hudault, Florian Chain, Olivier Berteau, Vasco Azevedo, Jean M Chatel, Harry Sokol, Luis G Bermúdez-Humarán, Muriel Thomas, Philippe Langella
Faecalibacterium prausnitzii is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. F. prausnitzii depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importance in human health, only few microbiological studies have been performed to isolate novel F. prausnitzii strains in order to better understand the biodiversity and physiological diversity of this beneficial commensal species...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28712577/bacterial-adherence-to-graft-tissues-in-static-and-flow-conditions
#15
Tiago Rafael Veloso, Jorien Claes, Soetkin Van Kerckhoven, Bartosz Ditkowski, Luis G Hurtado-Aguilar, Stefan Jockenhoevel, Petra Mela, Ramadan Jashari, Marc Gewillig, Marc F Hoylaerts, Bart Meyns, Ruth Heying
BACKGROUND: Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE...
June 16, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28712023/selection-of-potential-probiotic-lactobacillus-with-inhibitory-activity-against-salmonella-and-fecal-coliform-bacteria
#16
Mattika Abhisingha, Jureeporn Dumnil, Chetsadaporn Pitaksutheepong
Three hundred and sixty presumptive lactic acid bacteria (LAB) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-Salmonella activity. Fifty-eight isolates consisting of Lactobacillus reuteri (n = 32), Lactobacillus salivarius (n = 10), Lactobacillus mucosae (n = 8), Lactobacillus johnsonii (n = 5), and Lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substances, acid and bile tolerance, and cell adherence to Caco-2 cells...
July 15, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28711820/integrated-metagenomics-and-molecular-ecological-network-analysis-of-bacterial-community-composition-during-the-phytoremediation-of-cadmium-contaminated-soils-by-bioenergy-crops
#17
Zhaojin Chen, Yuan Zheng, Chuanyu Ding, Xuemin Ren, Jian Yuan, Feng Sun, Yuying Li
Two energy crops (maize and soybean) were used in the remediation of cadmium-contaminated soils. These crops were used because they are fast growing, have a large biomass and are good sources for bioenergy production. The total accumulation of cadmium in maize and soybean plants was 393.01 and 263.24μg pot(-1), respectively. The rhizosphere bacterial community composition was studied by MiSeq sequencing. Phylogenetic analysis was performed using 16S rRNA gene sequences. The rhizosphere bacteria were divided into 33 major phylogenetic groups according to phyla...
July 13, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28711240/characterization-and-bioactivities-of-the-exopolysaccharide-from-a-probiotic-strain-of-lactobacillus-plantarum-wlpl04
#18
Zhengqi Liu, Zhihong Zhang, Liang Qiu, Fen Zhang, Xiongpeng Xu, Hua Wei, Xueying Tao
Exopolysaccharide (EPS) was extracted and purified from Lactobacillus plantarum WLPL04, which has been confirmed previously as a potential probiotic for its antagonistic and immune-modulating activity. It has a molecular weight of 6.61 × 10(4) Da, consisting of xylose, glucose, and galactose in an approximate molar ratio of 3.4: 1.8: 1. Microstructural studies demonstrated that the EPS appeared as a smooth sheet structure with many homogeneous rod-shaped lumps. The preliminary in vitro assays indicated that the EPS could significantly inhibit the adhesion of Escherichia coli O157:H7 to HT-29 cells in competition, replacement, and inhibition assays at a dose of 1...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28708293/investigation-on-the-antimicrobial-activities-of-gingers-etlingera-coccinea-blume-s-sakai-nagam-and-etlingera-sessilanthera-r-m-sm-endemic-to-borneo
#19
Delhousie Daniel-Jambun, Jacky Dwiyanto, Yau Yan Lim, Joash Ban Lee Tan, Azira Muhamad, Sau Wai Yap, Sui Mae Lee
AIMS: To investigate the antimicrobial properties of E. coccinea and E. sessilanthera and to isolate and identify the antimicrobial compounds. METHODS AND RESULTS: Extracts were obtained via sequential solvent extraction method using hexane, dichloromethane, ethyl acetate, methanol and water. Antimicrobial activity testing was done using broth microdilution assay against 17 strains of bacteria. The leaf hexane extract of E. coccinea and rhizome hexane extract of E...
July 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28705747/synthesis-of-phytochemicals-stabilized-gold-nanoparticles-and-their-biological-activities-against-bacteria-and-leishmania
#20
Aftab Ahmad, Yun Wei, Sadeeq Ullah, Sayyed Ibrahim Shah, Fazli Nasir, Akram Shah, Zafar Iqbal, Kamran Tahir, Usman Ali Khan, Qipeng Yuan
Nanoscale materials have shown promising results in the field of medicine as therapeutic agents and drugs delivery vehicles. In the current study, gold nanoparticles (AuNPs) were prepared by a green and facile method using the aqueous extract of Rhazya stricta decne as a source of reducing and stabilizing agents. The bio-fabricated AuNPs were characterized by UV-visible spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM) and FTIR spectroscopy. Antimicrobial activities of the biosynthesized AuNPs were tested against Leishmania tropica (HTD7), E...
July 11, 2017: Microbial Pathogenesis
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