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https://www.readbyqxmd.com/read/27897272/analysis-of-the-interaction-of-calcitriol-with-the-disulfide-isomerase-erp57
#1
Elisa Gaucci, Domenico Raimondo, Caterina Grillo, Laura Cervoni, Fabio Altieri, Giulio Nittari, Margherita Eufemi, Silvia Chichiarelli
Calcitriol, the active form of vitamin D3, can regulate the gene expression through the binding to the nuclear receptor VDR, but it can also display nongenomic actions, acting through a membrane-associated receptor, which has been discovered as the disulfide isomerase ERp57. The aim of our research is to identify the binding sites for calcitriol in ERp57 and to analyze their interaction. We first studied the interaction through bioinformatics and fluorimetric analyses. Subsequently, we focused on two protein mutants containing the predicted interaction domains with calcitriol: abb'-ERp57, containing the first three domains, and a'-ERp57, the fourth domain only...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875514/domino-flaps-for-repairing-of-secondary-bilateral-cleft-lip-with-severe-columella-deformity
#2
Jiao Wei, Cheng-An Chiang, Da Zhou, Qingfeng Li, Kai Liu
The patients with secondary bilateral cleft lip and nasal deformity always suffer from a serious part of the horizontal and vertical soft tissue deficiencies in the upper lip and nose, especially the columella. Normally, the Abbe flap is used to increase the soft tissue volume of upper lip. However, how to reconstruct the sever columella deficient, how to make full use of the Abbe flap and the remaining soft tissue of the upper lip, are always the problems that need to be solved by plastic surgeons. Twenty-nine patients with secondary bilateral cleft lip and severe columella deficiency were simultaneously reconstructed with several local flaps like dominos that was called "Domino sliding flaps...
November 21, 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27869451/internet-delivered-family-based-treatment-for-early-onset-ocd-a-pilot-randomized-trial
#3
Jonathan S Comer, Jami M Furr, Caroline E Kerns, Elizabeth Miguel, Stefany Coxe, R Meredith Elkins, Aubrey L Carpenter, Danielle Cornacchio, Christine E Cooper-Vince, Mariah DeSerisy, Tommy Chou, Amanda L Sanchez, Muniya Khanna, Martin E Franklin, Abbe M Garcia, Jennifer B Freeman
Objective: Despite advances in supported treatments for early onset obsessive-compulsive disorder (OCD), progress has been constrained by regionally limited expertise in pediatric OCD. Videoteleconferencing (VTC) methods have proved useful for extending the reach of services for older individuals, but no randomized clinical trials (RCTs) have evaluated VTC for treating early onset OCD. Method: RCT comparing VTC-delivered family based cognitive-behavioral therapy (FB-CBT) versus clinic-based FB-CBT in the treatment of children ages 4-8 with OCD (N = 22)...
November 21, 2016: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/27869039/the-role-of-rs1984112_g-at-cd36-gene-in-increasing-reticulocyte-level-among-sickle-cell-disease-patients
#4
Miniar Kalai, Marwa Dridi, Leila Chaouch, Imen Moumni, Houyem Ouragini, Imen Darragi, Imen Boudrigua, Dorra Chaouachi, Fethi Mellouli, Mohamed Bejaoui, Salem Abbes
AIMS AND BACKGROUND: Mediators of adhesion become a potential new target for pharmacological therapy to struggle the complications of sickle cell disease (SCD). Several mechanisms for increased adherence have been postulated and the well-studied are CD36 and VLA4 which encoded by ITGA4. Herein, we sought to determine whether one polymorphism of CD36 namely: rs1984112 and three exons of ITGA4 (4, 5, and 6) are implicated in hemolytic status and clinical events among SCD Tunisian patients...
November 20, 2016: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/27867388/transcriptomic-changes-of-drought-tolerant-and-sensitive-banana-cultivars-exposed-to-drought-stress
#5
Muthusamy Muthusamy, Subbaraya Uma, Suthanthiram Backiyarani, Marimuthu Somasundaram Saraswathi, Arumugam Chandrasekar
In banana, drought responsive gene expression profiles of drought-tolerant and sensitive genotypes remain largely unexplored. In this research, the transcriptome of drought-tolerant banana cultivar (Saba, ABB genome) and sensitive cultivar (Grand Naine, AAA genome) was monitored using mRNA-Seq under control and drought stress condition. A total of 162.36 million reads from tolerant and 126.58 million reads from sensitive libraries were produced and mapped onto the Musa acuminata genome sequence and assembled into 23,096 and 23,079 unigenes...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27863306/surface-modified-biochar-in-a-bioretention-system-for-escherichia-coli-removal-from-stormwater
#6
Abbe Y T Lau, Daniel C W Tsang, Nigel J D Graham, Yong Sik Ok, Xin Yang, Xiang-Dong Li
Bioretention systems have been recommended as one of the best management practices for low impact development for water recycling/reuse systems. Although improvement of the stormwater quality has been reported regarding pollutants eliminations such as suspended solids and heavy metals, a substantial removal of indicator bacteria is required for possible non-potable reuse. This study investigated the efficiency of wood biochar with H2SO4-, H3PO4-, KOH-, and amino-modifications for E. coli removal from synthetic stormwater under intermittent flow...
November 15, 2016: Chemosphere
https://www.readbyqxmd.com/read/27828460/super-resolution-from-pure-hybrid-nanoscale-solid-immersion-lenses-under-dark-field-illumination
#7
Liyong Jiang, Wei Zhang, Hua Yuan, Xiangyin Li
We report a super-resolution strategy based on pure/hybrid nanoscale solid immersion lenses (n-SILs) under dark-field illumination. Dependences of the focusing performance of pure n-SILs on different diameters, locations, and substrates are investigated. Simulation results demonstrate a higher resolution (up to a 22.8% improvement) of pure n-SILs under dark-field illumination than convergent plane wave illumination. A hybrid n-SIL with a higher-index nanorod embedded into the n-SIL is proposed. Under dark-field illumination, the hybrid n-SIL can generate a near-field focal spot with a much higher resolution (~λ/8) that is beyond the Abbe diffraction limit in the nanorod...
October 31, 2016: Optics Express
https://www.readbyqxmd.com/read/27828139/immersed-diffraction-grating-refractometers-of-liquids
#8
L F G Dib, E A Barbosa
In this work, we report the development, construction, and the performance of two liquid refractometers that use a reflective diffraction grating immersed in a test liquid. The liquid is contained in a transparent glass cell with a rectangular cross section. The grating is oriented in such a way that the propagation directions of the incident beam and the beam diffracted by the lower part of the grating immersed in the liquid are perpendicular. In this configuration, the refractive index is determined by measuring the angle of the zeroth-order diffraction beam coming from the upper part of the grating, which is in contact with air...
October 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27826484/vermilion-only-cross-lip-flap-for-treating-whistle-deformity-in-secondary-bilateral-cleft-lip-repair
#9
Clifford C Sheckter, Robert M Menard
Bilateral cleft lip repairs can result in various secondary deformities. One more commonly seen deformity, the whistle deformity, is characterized by a reduced or absent tubercle, orbicularis muscle diastasis, and abnormalities of the philtrum with notched appearance of cupid's bow. Various techniques have been described to address these problems. One common procedure is the lip-switch flap originally described by Abbe in 1898, which has been modified by various surgeons. In these procedures, lower lip vermilion, mucosa, orbicularis, and lip skin are transposed to the upper lip on a pedicle that is later divided...
October 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27826477/reconstructive-approach-to-an-acquired-absence-of-multiple-facial-components-a-case-report
#10
Lauren N Oliver, Kongkrit Chaiyasate
The subject of our case report is a young girl who was attacked by a raccoon when she was 5 months old. She lost the majority of her nose, her entire right ear, and part of her upper lip. She had previous attempts at reconstructive surgery with poor results. Our collective goals were to provide lifelong reconstruction without the use of synthetic implants and to organize the steps in a way that provided psychological benefit early on while allowing her to continue education and childhood activities with minimal disruptions...
October 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27825294/downregulation-of-mir-451-in-tunisian-chronic-myeloid-leukemia-patients-potential-implication-in-imatinib-resistance
#11
Ismael Soltani, Kais Douzi, Hanen Gharbi, Islem Benhassine, Mouheb Teber, Hassiba Amouri, Hind Ben Hadj Othman, Ahlem Farrah, Raihane Ben Lakhel, Salem Abbes, Samia Menif
OBJECTIVES: Resistance to imatinib has been recognized as a major challenge for the treatment of chronic myeloid leukemia (CML). Aberrant expression of miR-451 has been reported to participate in anticancer drug resistance. However, the role of miR-451 in imatinib resistance has not been investigated. The present study was undertaken to determine the expression of miR-451 in order to find a possible association between the expression of this miRNA and imatinib resistance in Tunisian CML patients...
November 9, 2016: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/27816344/-a-psychiatric-disorder-reveals-a-neurobrucellosis
#12
Inès Feki, Wafa Abbes, Salma Sakka, Imen Bouchhima, Chokri Mhiri, Jaweher Masmoudi
No abstract text is available yet for this article.
November 2, 2016: La Presse Médicale
https://www.readbyqxmd.com/read/27803992/planomonospora-algeriensis-sp-nov-an-actinobacterium-isolated-from-a-saharan-soil-of-algeria
#13
Fawzia Chaabane Chaouch, Noureddine Bouras, Salim Mokrane, Khaoula Bouznada, Abdelghani Zitouni, Gabriele Pötter, Cathrin Spröer, Hans-Peter Klenk, Nasserdine Sabaou
A filamentous actinobacterium, designated strain PM3(T), was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3(T). The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore...
November 1, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27795340/comparison-of-magpix%C3%A2-%C3%A2-assays-and-elisa-for-the-detection-of-hemorrhagic-fever-viruses
#14
Neal G Satterly, Matthew A Voorhees, Abbe D Ames, Randal J Schoepp
Viral hemorrhagic fevers with their high mortality rates, lack of medical countermeasures, and potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detection. For decades, the gold-standard immunoassay for hemorrhagic fever detection has been the ELISA; however newer technologies are emerging with increased sensitivity. One such technology is the Luminex MAGPIX®™ platform using XMAP® microspheres...
October 19, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27783656/nonlinear-structured-illumination-using-a-fluorescent-protein-activating-at-the-readout-wavelength
#15
Hui-Wen Lu-Walther, Wenya Hou, Martin Kielhorn, Yoshiyuki Arai, Takeharu Nagai, Michael M Kessels, Britta Qualmann, Rainer Heintzmann
Structured illumination microscopy (SIM) is a wide-field technique in fluorescence microscopy that provides fast data acquisition and two-fold resolution improvement beyond the Abbe limit. We observed a further resolution improvement using the nonlinear emission response of a fluorescent protein. We demonstrated a two-beam nonlinear structured illumination microscope by introducing only a minor change into the system used for linear SIM (LSIM). To achieve the required nonlinear dependence in nonlinear SIM (NL-SIM) we exploited the photoswitching of the recently introduced fluorophore Kohinoor...
2016: PloS One
https://www.readbyqxmd.com/read/27760042/use-of-elements-of-the-stewart-model-strong-ion-approach-sid3-sid4-atot-a-side-and-sig-for-the-diagnostics-of-respiratory-acidosis-in-brachycephalic-dogs
#16
P Sławuta, G Sapikowski, B Sobieraj
: Buffer systems of blood and tissues, which have the ability to bind with and give up hydrogen ions, participate in maintaining the acid-base balance (ABB) of the organism. According to the classic model, the system of carbonic acid and bicarbonates, where the first component serves the role of an acid and the second a base, determines plasma pH. The so-called Stewart model, which assumes that ions in blood serum can be separated into completely dissociated - nonbuffer and not dissociated - buffer ions which may give up or accept H+ions, also describes the ABB of the organism...
September 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27730342/myocardial-and-liver-iron-overload-assessed-using-t2-magnetic-resonance-imaging-with-an-excel-spreadsheet-for-post-processing-in-tunisian-thalassemia-major-patients
#17
Monia Ouederni, Monia Ben Khaled, Fethi Mellouli, Elhem Ben Fraj, Nawel Dhouib, Ismehen Ben Yakoub, Selem Abbes, Nejla Mnif, Mohamed Bejaoui
Thalassemia is a common genetic disorder in Tunisia. Early iron concentration assessment is a crucial and challenging issue. Most of annual deaths due to iron overload occurred in underdeveloped regions of the world. Limited access to liver and heart MRI monitoring might partially explain these poor prognostic results. Standard software programs are not available in Tunisia. This study is the first to evaluate iron overload in heart and liver using the MRI T2* with excel spreadsheet for post processing. Association of this MRI tool results to serum ferritin level, and echocardiography was also investigated...
October 12, 2016: Annals of Hematology
https://www.readbyqxmd.com/read/27709623/refractive-indices-of-organo-metallic-and-metalloid-compounds-a-long-range-corrected-dft-study
#18
Shintaro Maekawa, Krzysztof Moorthi, Yasuteru Shigeta
Refractive indices of metal- and metalloid-containing compounds are systematically evaluated using the Lorentz-Lorenz equation with polarizabilities obtained via density functional theory (DFT). Among exchange-correlation functionals studied, the long-range corrected (LC) fuctionals yield the lowest errors for the polarizabilities of gaseous compounds and refractive indices of liquids. The LC-DFT predicts very well the wavelength dependence of refractive indices. A scheme for computing Abbe numbers of organometallic and organometaloid compounds is proposed and a refractive index - Abbe number plot for 80 compounds is constructed...
December 15, 2016: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/27700047/polymer-colloidal-sphere-based-hybrid-solid-immersion-lens-for-optical-super-resolution-imaging
#19
Haie Zhu, Wen Fan, Shuxue Zhou, Min Chen, Limin Wu
Optical microscope is widely used real-time investigation tool, but usually suffers from low resolution due to Abbe diffraction limit. Herein, we design and successfully synthesize ZrO2/polymer hybrid colloidal microspheres with as high as 47.5 wt% inorganic nanoparticles by suspension polymerization of 9,9'-bis[4-(2-acryloyloxyethyloxy) phenyl] fluorene (BAEPF). Owing to the homogeneous dispersion, high density and high refractive index of inorganic nanoparticles and deformability of polymer, the obtained ZrO2/poly(BAEPF) hybrid colloidal microspheres have high refractive index, optical transparency and controllable curvature, and thus can be directly used as a hybrid solid immersion lens (hSIL) for optical microscope, achieving super-resolution imaging of 50 nm even 45 nm under a standard white light or blue light optical microscopes, which is far beyond the diffraction limit for visible light optical microscopes...
October 4, 2016: ACS Nano
https://www.readbyqxmd.com/read/27684498/using-family-based-exposure-with-response-prevention-to-treat-obsessive-compulsive-disorder-in-young-children-a-case-study
#20
Jenny Herren, Jennifer Freeman, Abbe Garcia
Cognitive-behavioral therapy (CBT) using exposure with response prevention (ERP) is the treatment of choice for obsessive-compulsive disorder (OCD); however, developmental modifications should be considered when treating young children. This article presents a case study illustrating family-based CBT using ERP with a 7-year-old boy. The delivery of ERP for this case was guided by 3 main principles: (a) family involvement with a focus on reducing family accommodation, (b) understanding the functional relation between the client's obsessions and compulsions, and (c) creating conditions to facilitate habituation during exposure...
November 2016: Journal of Clinical Psychology
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