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Sílvia Rocha-Rodrigues, Amaia Rodríguez, Sara Becerril, Beatriz Ramírez, I O Gonçalves, Jorge Beleza, Gema Frühbeck, António Ascensão, José Magalhães
We aimed to investigate the effects of two physical exercise models, voluntary physical activity (VPA) and endurance training (ET) as preventive and therapeutic strategies, respectively, on lipid accumulation regulators and mitochondrial content in VAT of rats fed a high-fat diet (HFD). Sprague Dawley rats (6 wks old, n=60) were assigned into sedentary and VPA groups fed isoenergetic diets: standard (S, 35 kcal% fat) or HFD (71 kcal% fat). The VPA groups had free access to wheel running during the entire protocol...
November 22, 2016: Clinical and Experimental Pharmacology & Physiology
Jesper S Hansen, Christian Krintel, Malin Hernebring, Tatu J K Haataja, Sofia de Marè, Sebastian Wasserstrom, Urszula Kosinska-Eriksson, Madelene Palmgren, Cecilia Holm, Karin G Stenkula, Helena A Jones, Karin Lindkvist-Petersson
Accumulating evidence suggests that dysregulated glycerol metabolism contributes to the pathophysiology of obesity and type 2 diabetes. Glycerol efflux from adipocytes is regulated by the aquaglyceroporin AQP7, which is translocated upon hormone stimulation. Here, we propose a molecular mechanism where the AQP7 mobility in adipocytes is dependent on perilipin 1 and protein kinase A. Biochemical analyses combined with ex vivo studies in human primary adipocytes, demonstrate that perilipin 1 binds to AQP7, and that catecholamine activated protein kinase A phosphorylates the N-terminus of AQP7, thereby reducing complex formation...
December 2016: Metabolism: Clinical and Experimental
Jeanne Durendale Chiadak, Tatjana Arsenijevic, Francoise Gregoire, Nargis Bolaky, Valerie Delforge, Jason Perret, Christine Delporte
Aquaglyceroporins, belonging to the family of aquaporins (AQPs), are integral plasma membrane proteins permeable to water and glycerol that have emerged as key players in obesity. The aim of this study was to investigate the expression profile of AQPs in undifferentiated and differentiated 3T3-L1 cells and to investigate the changes in expression of aquaglyceroporins in 3T3-L1 cells differentiated into adipocytes and subjected to lipopolysaccharide (LPS) mimicking inflammation occurring during obesity. Furthermore, the study aimed at identifying the signaling cascade involved in the regulation of aquaglyceroporins expression upon LPS stimulation...
October 18, 2016: International Journal of Molecular Sciences
Tania Quesada-López, Laura González-Dávalos, Enrique Piña, Ofelia Mora
Adipose Tissue (AT) is a complex organ with a crucial regulatory role in energy metabolism and in the development of obesity and the Metabolic Syndrome (MS). Modified responses and the metabolism of hormones have been observed in visceral adiposity during obesity, specifically as related with cortisone. The objective of this study was to assess, in the 3T3-L1 adipocyte cell line, the short-term effect of cortisone on the expression of 11β-Hydroxysteroid dehydrogenase 1 (Hsd1), which is responsible for activation of cortisone into cortisol, and for Aquaporin 7 (Aqp7), involved in glycerol transport through the cell membrane...
July 2016: Adipocyte
Cui Zhu, Zhuang Chen, Zongyong Jiang
Stomach and intestines are involved in the secretion of gastrointestinal fluids and the absorption of nutrients and fluids, which ensure normal gut functions. Aquaporin water channels (AQPs) represent a major transcellular route for water transport in the gastrointestinal tract. Until now, at least 11 AQPs (AQP1-11) have been found to be present in the stomach, small and large intestines. These AQPs are distributed in different cell types in the stomach and intestines, including gastric epithelial cells, gastric glands cells, absorptive epithelial cells (enterocytes), goblet cells and Paneth cells...
2016: International Journal of Molecular Sciences
Mariusz T Skowronski, Agnieszka Skowronska, Aleksandra Rojek, Michal K Oklinski, Søren Nielsen
Aquaporins (AQPs) are membrane proteins involved in the regulation of cellular transport and the balance of water and glycerol and cell volume in the white adipose tissue (WAT). In our previous study, we found the co-expression of the AQP1 water channel and AQP7 in the mouse WAT. In our present study, we aimed to find out whether prolonged starvation influences the AQP1 expression of AQP7 knock-out mice (AQP7 KO) in the WAT. To resolve this hypothesis, immunoperoxidase, immunoblot and immunogold microscopy were used...
2016: International Journal of Molecular Sciences
Orkide Palabiyik, Aziz Karaca, Ebru Taştekin, Bilge Eren Yamasan, Burcu Tokuç, Tammam Sipahi, Selma Arzu Vardar
High-protein (HP) diets are commonly consumed by athletes despite their potential health hazard, which is postulated to enforce a negative effect on bone and renal health. However, its effects on heart have not been known yet. Aquaporin-7 (AQP7) is an aquaglyceroporin that facilitates glycerol and water transport. Glycerol is an important cardiac energy production substrate, especially during exercise, in conjunction with fatty acids and glucose. Glucose transporter 4 (GLUT4) is an insulin-sensitive glucose transporter in heart...
October 2016: Biochemical Genetics
K Michalek
Aquaporins belong to a family of small, transmembrane proteins that form channels selectively permeable for water. Some of them known as aquaglyceroporins also enable transportation of other small molecules such as glycerol, urea or ammonia. To date, 13 isoforms of aquaporins has been discovered in mammals (AQP0 - AQP12), 9 of which is localized in different parts of the renal tubular epithelium. In recent years, particular interest has been paid to aquaporins selectively permeable only to water molecules, determination of their localization and expression allowed to define the role of these proteins in renal excretion of water and their importance in the development of diseases...
April 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
Noelia Prieto-Martínez, Roser Morató, Rodrigo Muiño, Carlos O Hidalgo, Joan E Rodríguez-Gil, Sergi Bonet, Marc Yeste
The present study aimed to determine the localisation of aquaglyceroporins 3 (AQP3) and 7 (AQP7) in bull spermatozoa and their relationship with the sperm cell's resilience to withstand cryopreservation (i.e. cryotolerance). A total of 18 bull ejaculates were cryopreserved and their sperm quality analysed before and after freeze-thawing. The presence and localisation of AQP3 and AQP7 was determined through immunoblotting and immunocytochemistry. AQP3 was found in the mid-piece and AQP7 in the mid-piece and post-acrosomal region of bull spermatozoa...
May 25, 2016: Reproduction, Fertility, and Development
X J Tan, H Huang, F He, L Zhu, H Li, Y S Jiang, H Li, X H Huang, Z S Sun, Z H Li
OBJECTIVE: To define the potential causative gene mutation in a Chinese pedigree with progressive cardiac conduction defect (PCCD). METHODS: Sanger sequencing was performed to define potential causative gene mutation in a four-generation family with 68 members including seven PCCD patients (5 male) from 2010 to 2015.No causative gene was detected by screening known candidate genes related to PCCD including SCN5A, NKX2.5 and LMNA.High-throughput sequencing technology on exon-enriched DNA was then used to search the causative genes in 2 patients and one normal family member...
May 24, 2016: Zhonghua Xin Xue Guan Bing za Zhi
Aziz Karaca, Orkide Palabıyık, Ebru Taştekin, Fatma Nesrin Turan, Selma Arzu Vardar
OBJECTIVE: Cardiac uptake of fructose is thought to be mediated by glucose transporter 5 (GLUT5), whereas the uptake of glycerol is facilitated by aquaporin 7 (AQP7). We aimed to investigate the effect of a high-fructose diet (HFD) on GLUT5 and AQP7 levels in the rat heart subjected to exercise. METHODS: Male Sprague-Dawley rats were allocated to control (C; n=11), exercise (E; n=10), HFD (n=12), and HFD plus exercise (HFD-E; n=12) groups. HFD was started 28 days before euthanasia...
April 26, 2016: Anatolian Journal of Cardiology
Xiaohua Fu, Lili Xing, Weihai Xu, Jing Shu
Recent evidence has suggested that the marked decrease in ovarian secretion of estrogens in postmenopausal women may be associated with the development of non-alcoholic fatty liver disease. The present study aimed to elucidate the mechanisms by which low levels of estrogen induce fatty liver disease using an ovariectomized (OVX) mouse model and an in vitro cell model. A total of 24 female C57/BL6 mice were divided into four groups: Sham operation, sham operation plus subcutaneous implantation of tamoxifen (TAM), bilateral OVX, and OVX plus subcutaneous implantation of 17β-estradiol (E2)...
July 2016: Molecular Medicine Reports
Xiao-Feng Chen, Chuan-Fei Li, Lin Lü, Zhe-Chuan Mei
Aquaglyceroporins (AQPs) are a subset of the aquaporin family, and are permeable to water and glycerol. The aim of the present study was to determine the expression and clinical significance of three AQPs, AQP3, 7 and 9 in hepatocellular carcinoma (HCC). Fresh HCC and adjacent non‑tumorous liver tissues were collected from 68 patients diagnosed with HCC. The expression levels of AQP3, 7 and 9 were detected by reverse transcription‑quantitative polymerase chain reaction, western blotting and immunohistochemical analysis...
June 2016: Molecular Medicine Reports
Michelle L Krishnan, Zi Wang, Matt Silver, James P Boardman, Gareth Ball, Serena J Counsell, Andrew J Walley, Giovanni Montana, Anthony David Edwards
BACKGROUND: The consequences of preterm birth are a major public health concern with high rates of ensuing multisystem morbidity, and uncertain biological mechanisms. Common genetic variation may mediate vulnerability to the insult of prematurity and provide opportunities to predict and modify risk. OBJECTIVE: To gain novel biological and therapeutic insights from the integrated analysis of magnetic resonance imaging and genetic data, informed by prior knowledge...
July 2016: Brain and Behavior
A Rodríguez, J Gómez-Ambrosi, V Catalán, S Ezquerro, L Méndez-Giménez, S Becerril, P Ibáñez, N Vila, M A Margall, R Moncada, V Valentí, C Silva, J Salvador, G Frühbeck
BACKGROUND/OBJECTIVES: Uroguanylin and guanylin are secreted by intestinal epithelial cells as prohormones postprandially and act on the hypothalamus to induce satiety. The impact of obesity and obesity-associated type 2 diabetes (T2D) on proguanylin and prouroguanylin expression/secretion as well as the potential role of guanylin and uroguanylin in the control of lipolysis in humans was evaluated. SUBJECTS/METHODS: Circulating and gastrointestinal expression of proguanylin (GUCA2A) and prouroguanylin (GUCA2B) were measured in 134 subjects...
September 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Hee Jun Lee, Byung Chul Jee, Seul Ki Kim, Hoon Kim, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
STUDY QUESTION: Are mRNAs for specific aquaporins (AQPs) expressed in human luteinized granulosa cells (GCs) and are their expression levels correlated with in vitro fertilization (IVF) outcomes? SUMMARY ANSWER: The mRNAs of AQP1-7, 9, 11, and 12 were expressed in human luteinized GCs; the level of AQP1 mRNA was negatively associated with retrieved oocyte number and the level of AQP7 mRNA was positively associated with fertilization rate. WHAT IS KNOWN ALREADY: mRNAs of AQP1-4 and AQP9 have been detected in human GCs...
April 2016: Human Reproduction
Satoshi Hirako, Yoshihiro Wakayama, Hyounju Kim, Yuzuru Iizuka, Akiyo Matsumoto, Nobuhiro Wada, Ai Kimura, Mai Okabe, Junichi Sakagami, Mamiko Suzuki, Fumiko Takenoya, Seiji Shioda
Aquaporin (AQP) 7 and AQP9 are subcategorised as aquaglyceroporins which transport glycerin in addition to water. These AQPs may play a role in the homeostasis of energy metabolism. We examined the effect of AQP7, AQP9, and lipid metabolism-related gene expression in obese mice. In diet-induced obese (DIO) mice, excess lipid accumulated in the liver, which was hyperleptinemic and hyperinsulinemic. Hepatic AQP9 gene expression was significantly increased in both DIO and ob/ob mice compared to controls. The mRNA expression levels of fatty acid and triglyceride synthesis-related genes and fatty acid β oxidation-related genes in the liver were also higher in both mouse models, suggesting that triglyceride synthesis in this organ is promoted as a result of glycerol release from adipocytes...
November 2016: Obesity Research & Clinical Practice
Ya-Jing Tan, Xue-Ying Zhang, Guo-Lian Ding, Rong Li, Li Wang, Li Jin, Xian-Hua Lin, Ling Gao, Jian-Zhong Sheng, He-Feng Huang
Hyperosmotic stress may induce apoptosis of different cells. However, oocytes show tolerance to osmotic stress during cryopreservation by vitrification, which is an assisted reproductive technique. The underlying mechanism is still not understood. Here, we demonstrated that hyperosmosis produced by high concentrations of cryoprotectants, including DMSO, ethylene glycol and sucrose, significantly upregulated the protein levels of aquaporin (AQP) 7, but not AQP3 and AQP9, in mouse oocytes. Knockdown of AQP7 expression by siRNA-injection significantly reduced the survival of oocytes after vitrification...
December 4, 2015: Scientific Reports
Amaia Rodríguez, Raul A Marinelli, Angela Tesse, Gema Frühbeck, Giuseppe Calamita
Gender differences in the relative risk of developing metabolic complications, such as insulin resistance or non-alcoholic fatty liver disease (NAFLD), have been reported. The deregulation of glycerol metabolism partly contributes to the onset of these metabolic diseases, since glycerol constitutes a key substrate for the synthesis of triacylglycerols (TAGs) as well as for hepatic gluconeogenesis. The present mini-review covers the sex--related differences in glycerol metabolism and aquaglyceroporins (AQPs) and its impact in the control of adipose and hepatic fat accumulation as well as in whole-body glucose homeostasis...
2015: Frontiers in Endocrinology
Yuan-Hung Wang, Tsung-Ta Liu, Woon-Man Kung, Chun-Chi Chen, Ya-Ting Wen, I-Chan Lin, Chi-Chang Huang, Li Wei
Heat stroke (HS) has been shown to induce intestinal barrier dysfunction during whole body hyperthermia. HS-induced intestinal permeability change may result from modulation of aquaporin (AQP) expression, which subsequently regulates water homeostasis. This study aimed to evaluate AQP expression in the intestine of rats with HS at different recovery time points. Sprague-Dawley (SD) rats were exposed to an ambient temperature of 40 ± 0.5°C until a maximum core temperature of 40.5°C was attained. The small intestine was surgically removed and histologically examined, and AQP expression was determined by reverse transcription polymerase chain reaction and immunohistochemical staining...
2015: International Journal of Clinical and Experimental Pathology
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