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ABC family

SooHyun Park, Sheereen Majd
This paper describes the preparation of giant unilamellar vesicles with reconstituted hamster P-glycoprotein (Pgp, ABCB1) for studying the transport activity of this efflux pump in individual liposomes using optical microscopy. Pgp, a member of ABC (ATP-binding cassette) transporter family, is known to contribute to the cellular multidrug resistance (MDR) against variety of drugs. The efficacy of many therapeutics is, thus, hampered by this efflux pump, leading to a high demand for simple and effective strategies to monitor the interactions of candidate drugs with this protein...
2018: PloS One
Sravan Kumar Miryala, Sudha Ramaiah
In the present study, we have constructed an interaction network of 29 antibiotic resistant genes along with 777 interactions in E. coli O157:H7. Gene ontology analysis reveals that 94, 89 and 67 genes have roles in the cellular process, biological process and molecular function respectively. Gene complexes related to tripartite efflux pumps mdtEF-tolC and ABC family efflux pump macAB-tolC play key roles in multidrug efflux systems. It is noteworthy to mention that, 19 genes are involved in multi-efflux pumps and they play a significant role in multidrug resistance (MDR); while 18 genes are vital for fatty acid synthesis...
June 13, 2018: Genomics
Louise R Barreto, Thayná Barreto, Sonia Melo, Cristina Pungartnik, Martin Brendel
Pathogenesis-related proteins (PRs) are induced in plants after infection by pathogens and/or abiotic stress. Among these proteins, the family 10 (PR-10) influences the biosynthesis of secondary metabolites and shows antimicrobial ribonuclease activity. Tc PR-10p (Pathogenesis-related Protein 10 of Theobroma cacao ) was isolated from resistant and susceptible Moniliophthora perniciosa cacao cultivars. Cell survival with Saccharomyces cerevisiae mutant lines deficient in ATP-binding cassette (ABC) transporter proteins indicated the influence on resistance to Tc PR-10p...
June 13, 2018: Biology
Nicholas P Greene, Elise Kaplan, Allister Crow, Vassilis Koronakis
The MacB ABC transporter forms a tripartite efflux pump with the MacA adaptor protein and TolC outer membrane exit duct to expel antibiotics and export virulence factors from Gram-negative bacteria. Here, we review recent structural and functional data on MacB and its homologs. MacB has a fold that is distinct from other structurally characterized ABC transporters and uses a unique molecular mechanism termed mechanotransmission. Unlike other bacterial ABC transporters, MacB does not transport substrates across the inner membrane in which it is based, but instead couples cytoplasmic ATP hydrolysis with transmembrane conformational changes that are used to perform work in the extra-cytoplasmic space...
2018: Frontiers in Microbiology
Khedidja Mosbahi, Marta Wojnowska, Amaya Albalat, Daniel Walker
Iron is an essential micronutrient for most bacteria and is obtained from iron-chelating siderophores or directly from iron-containing host proteins. For Gram-negative bacteria, classical iron transport systems consist of an outer membrane receptor, a periplasmic binding protein, and an inner membrane ABC transporter, which work in concert to deliver iron from the cell surface to the cytoplasm. We recently showed that Pectobacterium spp. are able to acquire iron from ferredoxin, a small and stable 2Fe-2S iron sulfur cluster containing protein and identified the ferredoxin receptor, FusA, a TonB-dependent receptor that binds ferredoxin on the cell surface...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Angelika Długosz, Katarzyna Gach-Janczak, Jacek Szymański, Dariusz Deredas, Tomasz Janecki, Anna Janecka
BACKGROUND: The development of multidrug resistance to chemotherapy remains a challenge in the treatment of cancer and is a major factor causing failure of many forms of chemotherapy. The ATP binding cassette (ABC) family of proteins are efflux pumps that transport various potentially dangerous substances out of the cells. Several of the ABC transporters are related to chemoresistance, as the rapidly dividing malignant cells use them to protect themselves from medical interventions. Inhibitors of ABC transporters have the potential to enhance the efficacy of anticancer drugs...
January 10, 2018: Pharmacological Reports: PR
Charles Y Wang, Nisha Patel, Wei-Yun Wholey, Suzanne Dawid
The opportunistic pathogen Streptococcus pneumoniae (pneumococcus) uses natural genetic competence to increase its adaptability through horizontal gene transfer. One method of acquiring DNA is through predation of neighboring strains with antimicrobial peptides called "bacteriocins." Competence and production of the major family of pneumococcal bacteriocins, pneumocins, are regulated by the quorum-sensing systems com and blp , respectively. In the classical paradigm, the ABC transporters ComAB and BlpAB each secretes its own system's signaling pheromone and in the case of BlpAB also secretes the pneumocins...
June 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
Bartosz Roszniowski, Siobhán McClean, Zuzanna Drulis-Kawa
Burkholderia cenocepacia , is a Gram-negative opportunistic pathogen that belongs to Burkholderia cepacia complex (BCC) group. BCC representatives carry various pathogenicity factors and can infect humans and plants. Phages as bacterial viruses play a significant role in biodiversity and ecological balance in the environment. Specifically, horizontal gene transfer (HGT) and lysogenic conversion (temperate phages) influence microbial diversification and fitness. In this study, we describe the prevalence and gene content of prophages in 16 fully sequenced B...
May 31, 2018: Viruses
Behrooz Darbani, Douglas B Kell, Irina Borodina
BACKGROUND: Transporter proteins mediate the translocation of substances across the membranes of living cells. Many transport processes are energetically expensive and the cells use 20 to 60% of their energy to power the transportomes. We hypothesized that there may be an evolutionary selection pressure for lower energy transporters. RESULTS: We performed a genome-wide analysis of the compositional reshaping of the transportomes across the kingdoms of bacteria, archaea, and eukarya...
May 30, 2018: BMC Genomics
Xiuzhen Sheng, Min Liu, Haibo Liu, Xiaoqian Tang, Jing Xing, Wenbin Zhan
Streptococcus iniae is a major Gram-positive pathogen that causes invasive disease in fish worldwide. In this study, in order to identify immunogenic proteins for developing highly effective vaccine against S. iniae, whole-cell lysate proteins of S. iniae were analyzed by western blotting using flounder anti-S. iniae antibodies, and two positive protein bands of molecular weight 37 kDa and 40 kDa were screened, which were identified as pyruvate dehydrogenase E1 subunit alpha (PDHA1), BMP family ABC transporter substrate-binding protein (BMP) and L-lactate dehydrogenase (LDH), as well as ornithine carbamoyltransferase (OCT), lactate oxidas (LOx) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by mass spectrometry...
2018: PloS One
Xuemin Ma, Youjun Zhang, Veronika Turečková, Gang-Ping Xue, Alisdair R Fernie, Bernd Mueller-Roeber, Salma Balazadeh
Leaf senescence is an essential physiological process in plants that supports the recycling of nitrogen and other nutrients to support the growth of developing organs, including young leaves, seeds and fruits. Thus, the regulation of senescence is crucial for evolutionary success in wild populations and for increasing yield in crops. Here we describe the influence of a NAC transcription factor, SlNAP2 (Solanum lycopersicum NAC-like, activated by apetala3/pistillata), that controls both leaf senescence and fruit yield in tomato (Solanum lycopersicum)...
May 14, 2018: Plant Physiology
Xiaozhen Ye, Zhenhui Zhong, Hongyi Liu, Lianyu Lin, Mengmeng Guo, Wenshuo Guo, Zonghua Wang, Qinghua Zhang, Lizhen Feng, Guodong Lu, Feiping Zhang, Quanzhu Chen
BACKGROUND: Leaf blight caused by Calonectria spp. is one of the most destructive diseases to affect Eucalyptus nurseries and plantations. These pathogens mainly attack Eucalyptus, a tree with a diversity of secondary metabolites employed as defense-related phytoalexins. To unravel the fungal adaptive mechanisms to various phytoalexins, we examined the genome of C. pseudoreteaudii, which is one of the most aggressive pathogens in southeast Asia. RESULTS: A 63.7 Mb genome with 14,355 coding genes of C...
May 10, 2018: BMC Genomics
Giselda Bucca, Radhika Pothi, Andrew Hesketh, Carla Möller-Levet, David A Hodgson, Emma E Laing, Graham R Stewart, Colin P Smith
Stress-induced adaptations require multiple levels of regulation in all organisms to repair cellular damage. In the present study we evaluated the genome-wide transcriptional and translational changes following heat stress exposure in the soil-dwelling model actinomycete bacterium, Streptomyces coelicolor. The combined analysis revealed an unprecedented level of translational control of gene expression, deduced through polysome profiling, in addition to transcriptional changes. Our data show little correlation between the transcriptome and 'translatome'; while an obvious downward trend in genome wide transcription was observed, polysome associated transcripts following heat-shock showed an opposite upward trend...
May 9, 2018: Nucleic Acids Research
Ao Wu, Jiaoli Zhang, Liyan Zeng, Feng Li, Zhaoguang Dong, Yanling Feng, Xiaofang Jia, Jun-O Jin, Lijun Zhang
BACKGROUND: ATP-binding cassette sub-family G member 2 (ABCG2), an ABC transport protein involved in the efflux of anticancer compounds, has been reported to have altered expression levels in several cancers including breast, colon, and gastric cancer, etc. However, its expression change (up- or down-regulated in cancer) is still contradictory. METHODS: We performed immunohistochemistry to examine ABCG2 expression in the microarray with 90 pairs of colon cancer and their adjacent normal tissues...
March 1, 2018: Clinical Laboratory
Benjamin C Cheah, Jane Davies, Gurmeet R Singh, Nicholas Wood, Kathy Jackson, Margaret Littlejohn, Belinda Davison, Peter McIntyre, Stephen Locarnini, Joshua S Davis, Steven Y C Tong
BACKGROUND: In Australia's Northern Territory, the hepatitis B virus (HBV) subgenotype A2 (subtype adw2) vaccine was introduced in 1988 for Indigenous infants. Subsequently, the circulating viral genotype has been identified as subgenotype C4 (subtype ayw3). We assessed HBV vaccine effectiveness (VE) in light of this subtype mismatch. METHODS: Participants of the Aboriginal Birth Cohort (ABC) study were recruited at birth (1987-1990), with HBV serology obtained at follow-up waves 3 (2005-2007) and 4 (2013-2015)...
May 4, 2018: Vaccine
Juan Chen, Qi-Biao Su, Yu-Qian Tao, Jia-Ming Qin, Yi Zhou, Shan Zhou, Hong-Liang Li, Zhuo-Jia Chen, Ya-Fang Zhou, Lie-Min Zhou, Xue-Ding Wang, Min Huang
Valproic acid (VPA), a widely used antiepileptic drug, is characterized by intensive inter-individual variability in concentration. Both efflux and influx transporters are reported to play important roles in the disposition of VPA, however, no comprehensive investigation into the association of the single nucleotide polymorphism (SNP) in ABC/SLC families with VPA concentration are reported. In the present study, we investigated the association of 12 SNPs in ABCC2, ABCC4, ABCG2, MCT1, MCT2, and OATP2B1 in 187 Chinese patients with epilepsy on VPA monotherapy with the trough concentrations of VPA...
May 1, 2018: Die Pharmazie
Viera Bohacova, Mario Seres, Lucia Pavlikova, Szilvia Kontar, Martin Cagala, Pavel Bobal, Jan Otevrel, Julius Brtko, Zdena Sulova, Albert Breier
The acceleration of drug efflux activity realized by plasma membrane transporters in neoplastic cells, particularly by P-glycoprotein (P-gp, ABCB1 member of the ABC transporter family), represents a frequently observed molecular cause of multidrug resistance (MDR). This multiple resistance represents a real obstacle in the effective chemotherapy of neoplastic diseases. Therefore, identifying cytotoxic substances that are also effective in P-gp overexpressing cells may be useful for the rational design of substances for the treatment of malignancies with developed MDR...
May 1, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hoangdung Ho, Anh Miu, Mary Kate Alexander, Natalie K Garcia, Angela Oh, Inna Zilberleyb, Mike Reichelt, Cary D Austin, Christine Tam, Stephanie Shriver, Huiyong Hu, Sharada S Labadie, Jun Liang, Lan Wang, Jian Wang, Yan Lu, Hans E Purkey, John Quinn, Yvonne Franke, Kevin Clark, Maureen H Beresini, Man-Wah Tan, Benjamin D Sellers, Till Maurer, Michael F T Koehler, Aaron T Wecksler, James R Kiefer, Vishal Verma, Yiming Xu, Mireille Nishiyama, Jian Payandeh, Christopher M Koth
The movement of core-lipopolysaccharide across the inner membrane of Gram-negative bacteria is catalysed by an essential ATP-binding cassette transporter, MsbA. Recent structures of MsbA and related transporters have provided insights into the molecular basis of active lipid transport; however, structural information about their pharmacological modulation remains limited. Here we report the 2.9 Å resolution structure of MsbA in complex with G907, a selective small-molecule antagonist with bactericidal activity, revealing an unprecedented mechanism of ABC transporter inhibition...
May 2, 2018: Nature
Weixin Su, Veerendra Kumar, Yichen Ding, Rya Ero, Aida Serra, Benjamin Sian Teck Lee, Andrew See Weng Wong, Jian Shi, Siu Kwan Sze, Liang Yang, Yong-Gui Gao
The ribosome is one of the richest targets for antibiotics. Unfortunately, antibiotic resistance is an urgent issue in clinical practice. Several ATP-binding cassette family proteins confer resistance to ribosome-targeting antibiotics through a yet unknown mechanism. Among them, MsrE has been implicated in macrolide resistance. Here, we report the cryo-EM structure of ATP form MsrE bound to the ribosome. Unlike previously characterized ribosomal protection proteins, MsrE is shown to bind to ribosomal exit site...
April 30, 2018: Proceedings of the National Academy of Sciences of the United States of America
Irshad Ahmad, Nighat Nawaz, Fatemeh Karimi Dermani, Alisa Khodadadi Kohlan, Massoud Saidijam, Simon G Patching
Antimicrobial resistance is a global challenge, for which the mechanisms of resistance are varied and complicated. Bacterial multidrug efflux proteins are a major mechanism of antimicrobial resistance and therefore targets for drug development. Gram-negative bacteria are intrinsically more resistant to many antibiotics and biocides due to their cell structure and the activity of multidrug efflux proteins. These transporters actively extrude antibiotics and other xenobiotics from the cytoplasm or surrounding membranes of cells to the external environment...
April 25, 2018: Current Drug Targets
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