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https://www.readbyqxmd.com/read/28078493/false-positive-newborn-screen-using-the-beutler-spot-assay-for-galactosemia-in-glucose-6-phosphate-dehydrogenase-deficiency
#1
Grace Stuhrman, Stefanie J Perez Juanazo, Kea Crivelly, Jennifer Smith, Hans Andersson, Eva Morava
Classical galactosemia is detected through newborn screening by measuring galactose-1-phosphate uridylyltransferase (GALT) in the USA primarily via the Beutler spot assay. We report on an 18-month-old patient with glucose-6-phosphate dehydrogenase (G6PD) deficiency that was originally diagnosed with classical galactosemia. The patient presented with elevated liver function enzymes and bilirubinemia and was immediately treated with soy-based formula. Confirmatory tests revealed deficiency of the GALT enzyme, however, full-sequencing of GALT was normal, suggestive of a different ideology...
January 12, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28077330/dehydropachymic-acid-decreases-bafilomycin-a1-induced-%C3%AE-amyloid-accumulation-in-pc12-cells
#2
Mengyao Yu, Xiaoyan Xu, Nan Jiang, Wei Wei, Fang Li, Liming He, Xia Luo
ETHNOPHARMACOLOGICAL RELEVANCE: Fuling, the sclerotium of Poria cocos, was frequently used in traditional Chinese medicine (TCM) formulae for Alzheimer's disease (AD) intervention over the past 10 centuries. And its extracts exhibited significant effects in both cellular and animal models of AD in previous studies. However, its mechanisms on prevention and treatment of AD have not been well elucidated yet. AIM OF THE STUDY: To investigate the effect and corresponding mechanisms of dehydropachymic acid, which is one of the major triterpenes in Poria cocos, on the clearance of β-Amyloid accumulation in bafilomycin A1 induced PC12 cells...
January 7, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27959437/traditional-medicine-sobokchukeo%C3%A2-tang-modulates-the-inflammatory-response-in-adipocytes-and-macrophages
#3
Hoyoung Lee, Eun Hyoung Shim, Myeong Soo Lee, Chang-Seon Myung
Sobokchukeo-Tang (ST) is a well-known formula that is used for treating primary dysmenorrhea caused by blood stasis syndrome (BSS) in Korea and China. The current study investigated the anti‑inflammatory and anti‑adipogenesis effects of ST on adipocytes and macrophages. The anti‑inflammatory efficacy of ST was evaluated in RAW 264.7 cells and differentiated THP‑1 cells. To induce inflammation, the cells were treated with lipopolysaccharide (LPS; 1 µg/ml). Following the induction of inflammation, the levels of proinflammatory cytokines, interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) in the cell supernatant were detected using enzyme‑linked immunosorbent assay...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27924786/evolution-of-a-high-local-strain-in-rolling-up-mos2-sheets-decorated-with-ag-and-au-nanoparticles-for-surface-enhanced-raman-scattering
#4
Da Young Hwang, Dong Hack Suh
We report that a high local strain was obtained for multilayer MoS2 nanoscrolls decorated with noble nanoparticles (Ag and Au NPs) using a rolling process beyond breaking or slipping of MoS2. The local strain was estimated through the bending strain in the nanoscrolls and the extent of coverage of Ag and Au NPs decorated on MoS2, exhibiting magnified surface-enhanced Raman scattering. TEM images showed that the MoS2-Ag and MoS2-Au nanoscrolls have a tube-like morphology decorated with NPs on the inner and outer sides of the MoS2 nanoscrolls...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27921069/reaching-200-ps-timing-resolution-in-a-time-of-flight-and-depth-of-interaction-positron-emission-tomography-detector-using-phosphor-coated-crystals-and-high-density-silicon-photomultipliers
#5
Sun Il Kwon, Alessandro Ferri, Alberto Gola, Eric Berg, Claudio Piemonte, Simon R Cherry, Emilie Roncali
Current research in the field of positron emission tomography (PET) focuses on improving the sensitivity of the scanner with thicker detectors, extended axial field-of-view, and time-of-flight (TOF) capability. These create the need for depth-of-interaction (DOI) encoding to correct parallax errors. We have proposed a method to encode DOI using phosphor-coated crystals. Our initial work using photomultiplier tubes (PMTs) demonstrated the possibilities of the proposed method, however, a major limitation of PMTs for this application is poor quantum efficiency in yellow light, corresponding to the wavelengths of the converted light by the phosphor coating...
October 2016: Journal of Medical Imaging
https://www.readbyqxmd.com/read/27914519/growth-and-tolerance-of-infants-fed-formula-with-a-new-algal-source-of-docosahexaenoic-acid-double-blind-randomized-controlled-trial
#6
Michael Yeiser, Cheryl L Harris, Ashlee L Kirchoff, Ashley C Patterson, Jennifer L Wampler, Edward N Zissman, Carol Lynn Berseth
Docosahexaenoic acid (DHA) in infant formula at concentrations based on worldwide human milk has resulted in circulating red blood cell (RBC) lipids related to visual and cognitive development. In this study, infants received study formula (17mg DHA/100kcal) with a commercially-available (Control: n=140; DHASCO®) or alternative (DHASCO®-B: n=127) DHA single cell oil from 14 to 120 days of age. No significant group differences were detected for growth rates by gender through 120 days of age. Blood fatty acids at 120 days of age were assessed by capillary column gas chromatography in a participant subset (Control: n=34; DHASCO-B: n=27)...
December 2016: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/27862446/fine-tuning-the-fluorescence-gain-of-fret-type-bodipy-bodipy-nhc-iridium-complexes-for-co-detection-with-a-large-virtual-stokes-shift
#7
Oliver Halter, Israel Fernández, Herbert Plenio
Complexes of the general formula [IrCl(cod)(bdp-NHC-bdp')] and [IrCl(cod)(bdp-NHC)] (bdp=bodipy=4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, cod=1,5-cyclooctadiene) were synthesized. The substitution reaction of cod with two molecules of CO converts weakly fluorescent into strongly fluorescent complexes [IrCl(CO)2 (bdp-NHC-bdp')] and [IrCl(CO)2 (bdp-NHC)]. Bdp and bdp" form a fluorescence resonance energy transfer (FRET) pair and the excitation of bdp leads to a strong emission from bdp" with a virtual Stokes shift of 98 nm...
November 8, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27852129/changes-in-the-ginsenoside-content-during-fermentation-using-an-appliance-for-the-preparation-of-red-ginseng
#8
So Jin Lee, Na Ha, Yunjeong Kim, Min-Gul Kim
The total amount of ginsenoside in fermented red ginseng (FRG) is increased by microbial fermentation. The aim of this study was to evaluate whether fermentation time and temperature affect the ginsenoside content during fermentation using an appliance for the preparation of red ginseng. The FRG and fermented red ginseng extracts (FRG-e) were prepared using an appliance for the preparation of red ginseng. The temperature was recorded and time points for sampling were scheduled at pre-fermentation (0[Formula: see text]h) and 18, 36, 48, 60 and 72[Formula: see text]h after the addition of the microbial strains...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27785521/-the-cost-estimator-in-the-traumaregister-dgu
#9
R Lefering, L Mahlke, D Franz
BACKGROUND AND OBJECTIVES: Estimated treatment costs of severely injured patients were often added to registry-based analyses. In the past, the TraumaRegister DGU® used a modular cost estimator for this purpose. A recent research project initiated by the German Trauma Society (DGU) evaluated the reimbursement of severely injured patients in the German DRG system. This project also allowed the generation of an improved update of the registry's cost estimator. METHODS: Detailed cost data for the acute therapy of severely injured patients were available from 10 hospitals that also participate in the TraumaRegister DGU®...
October 26, 2016: Der Unfallchirurg
https://www.readbyqxmd.com/read/27776430/baicalein-promotes-angiogenesis-and-odontoblastic-differentiation-via-the-bmp-and-wnt-pathways-in-human-dental-pulp-cells
#10
Sang-Im Lee, Sun-Young Kim, Kyung-Ran Park, Eun-Cheol Kim
Baicalein is an active flavonoid extracted from the root of Scutellaria baicalensis that has anticancer and anti-inflammatory properties; its effects on osteoblastic and angiogenic potential are controversial. The aim of this study was to investigate the effects of baicalein on odontoblastic differentiation and angiogenesis and the underlying mechanism in human dental pulp cells (HDPCs). Baicalein (1-10[Formula: see text][Formula: see text]M) had no cytotoxic effects and promoted alkaline phosphatase (ALP) activity, mineralization assayed by Alizarin Red-S staining, and the mRNA expression of marker genes, in a concentration-dependent manner...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27764134/calcium-sensitive-fluorescent-dyes-fluo-4-and-fura-red-under-pressure-behaviour-of-fluorescence-and-buffer-properties-under-hydrostatic-pressures-up-to-200-mpa
#11
D Schneidereit, H Vass, B Reischl, R J Allen, O Friedrich
The fluorescent Ca2+ sensitive dyes Fura Red (ratiometric) and Fluo-4 (non-ratiometric) are widely utilized for the optical assessment of Ca2+ fluctuations in vitro as well as in situ. The fluorescent behavior of these dyes is strongly depends on temperature, pH, ionic strength and pressure. It is crucial to understand the response of these dyes to pressure when applying calcium imaging technologies in the field of high pressure bioscience. Therefore, we use an optically accessible pressure vessel to pressurize physiological Ca2+-buffered solutions at different fixed concentrations of free Ca2+ (1 nM to 25...
2016: PloS One
https://www.readbyqxmd.com/read/27738841/effects-of-flowing-rbcs-on-adhesion-of-a-circulating-tumor-cell-in-microvessels
#12
L L Xiao, Y Liu, S Chen, B M Fu
Adhesion of circulating tumor cells (CTCs) to the microvessel wall largely depends on the blood hydrodynamic conditions, one of which is the blood viscosity. Since blood is a non-Newtonian fluid, whose viscosity increases with hematocrit, in the microvessels at low shear rate. In this study, the effects of hematocrit, vessel size, flow rate and red blood cell (RBC) aggregation on adhesion of a CTC in the microvessels were numerically investigated using dissipative particle dynamics. The membrane of cells was represented by a spring-based network connected by elastic springs to characterize its deformation...
October 13, 2016: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/27726219/combined-cell-index-in-assessing-blood-donor-iron-stores
#13
T Vuk, J Bingulac-Popović, T Očić, L J Mayer, M Milošević, I Jukić
OBJECTIVES: The aim of this study was to assess the appropriateness of using combined cell index (CCI) in the assessment of iron stores in blood donors. This index is calculated by the formula: red blood cell distribution width (RDW) × 10(4) × mean corpuscular volume (MCV)(-1) × mean corpuscular haemoglobin (MCH)(-1) . BACKGROUND: Ferritin measurement is a reliable method for estimating iron stores in blood donors. The sensitivity of red blood cell (RBC) parameters of complete blood count in detecting non-anaemic iron deficiency is significantly lower...
October 11, 2016: Transfusion Medicine
https://www.readbyqxmd.com/read/27724091/rapid-emergency-and-unscheduled-care-phipps-oliver-and-lugg-jason-rapid-emergency-and-unscheduled-care-216pp-%C3%A2-21-99-wiley-blackwell-9781119035855-1119035856-formula-see-text
#14
(no author information available yet)
This book is an informative reference guide to pathologies, illnesses and some minor injuries that nurse practitioners, paramedics, doctors and allied health professionals are likely to deal with in their daily practice. Laid out in a logical way, its outlines red flags, risk factors, causes, assessment, diagnostics, and management of various conditions.
October 6, 2016: Emergency Nurse: the Journal of the RCN Accident and Emergency Nursing Association
https://www.readbyqxmd.com/read/27720547/hyperspectral-leaf-reflectance-of-carpinus-betulus-l-saplings-for-urban-air-quality-estimation
#15
Melanka Brackx, Shari Van Wittenberghe, Jolien Verhelst, Paul Scheunders, Roeland Samson
In urban areas, the demand for local assessment of air quality is high. The existing monitoring stations cannot fulfill the needs. This study assesses the potential of hyperspectral tree leaf reflectance for monitoring traffic related air pollution. Hereto, 29 Carpinus betulus saplings were exposed to an environment with either high or low traffic intensity. The local air quality was estimated by leaf saturation isothermal remanent magnetization (SIRM). The VIS-NIR leaf reflectance spectrum (350-2500 nm) was measured using a handheld AgriSpec spectroradiometer (ASD Inc...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27717907/standardization-of-the-manufacturing-procedure-for-pinelliae-rhizoma-praeparatum-cum-zingibere-et-alumine
#16
Tao Su, Wei-Wei Zhang, Ya-Ming Zhang, Brian Chi-Yan Cheng, Xiu-Qiong Fu, Ting Li, Hui Guo, Ya-Xi Li, Pei-Li Zhu, Hui Cao, Zhi-Ling Yu
ETHNOPHARMACOLOGICAL RELEVANCE: Pinelliae Rhizoma (PR), the dried tuber of Pinellia ternata (Thunb.) Breit., is a traditional Chinese medicinal herb. It is commonly used for treating cancer, cough and phlegm. To treat cancer, Chinese medicine practitioners often use raw PR; while to treat cough and phlegm, they usually use Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine (PRZA, raw PR processed with ginger juice and alumen as adjuvant materials). Currently, the producing protocol of PRZA varies greatly among different places in China...
December 4, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27703400/body-surface-area-a-predictor-of-response-to-red-blood-cell-transfusion
#17
Louise Man, H Raymond Tahhan
A current focus of transfusion medicine is a judicious strategy in transfusion of blood products. Unfortunately, our ability to predict hemoglobin (Hgb) response to transfusion has been limited. The objective of this study was to determine variability of response to red blood cell transfusion and to predict which patients will have an Hgb rise higher or lower than that predicted by the long-standing convention of "one and three". This was a retrospective chart review in a single hospital. Data for 167 consecutive patient encounters were reviewed...
2016: Journal of Blood Medicine
https://www.readbyqxmd.com/read/27676461/discovery-of-a-red-emitting-li3rbge8o18-mn-4-phosphor-in-the-alkali-germanate-system-structural-determination-and-electronic-calculations
#18
Satendra Pal Singh, Minseuk Kim, Woon Bae Park, Jin-Woong Lee, Kee-Sun Sohn
A solid-state combinatorial chemistry approach, which used the A-Ge-O (A = Li, K, Rb) system doped with a small amount of Mn(4+) as an activator, was adopted in a search for novel red-emitting phosphors. The A site may have been composed of either a single alkali metal ion or of a combination of them. This approach led to the discovery of a novel phosphor in the above system with the chemical formula Li3RbGe8O18:Mn(4+). The crystal structure of this novel phosphor was solved via direct methods, and subsequent Rietveld refinement revealed a trigonal structure in the P3̅1m space group...
October 17, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27670270/development-and-preliminary-psychometric-evaluation-of-decisional-balance-and-self-efficacy-measures-for-managing-anxiety-in-a-national-sample-of-clinically-anxious-adults
#19
Jessica M Lipschitz, Andrea L Paiva, Colleen A Redding, Deborah Levesque, Joseph S Rossi, Risa B Weisberg, James O Prochaska
PURPOSE: Anxiety is the most common and costly mental illness in the United States. Reducing avoidance is a core element of evidence-based treatments. Past research shows readiness to address avoidance affects outcomes. Investigating avoidance from a transtheoretical model (TTM) perspective could facilitate tailored approaches for individuals with low readiness. This study developed and examined psychometric properties of TTM measures for addressing anxiety-based avoidance. DESIGN: Cross-sectional survey...
September 26, 2016: American Journal of Health Promotion: AJHP
https://www.readbyqxmd.com/read/27669827/bioemulsifier-be-am1-produced-by-solibacillus-silvestris-am1-is-a-functional-amyloid-that-modulates-bacterial-cell-surface-properties
#20
A R Markande, A S Nerurkar
A novel estuarine bacterial strain, Solibacillus silvestris AM1, produces an extracellular, thermostable and fibrous, glycoprotein bioemulsifier (BE-AM1). The amyloid nature of the bioemulsifier (BE-AM1) was confirmed by biophysical techniques (Congo red based polarization microscopy, ThioflavinS based fluorescent microscopy, fibrous arrangement in transmission electron microscopy and secondary structure measurement by FTIR and CD spectrum analysis). Cell-bound BE-AM1 production by S. silvestris AM1 during the mid-logarithmic phase of growth coincided with a decrease in cell surface hydrophobicity, and an increase in cell autoaggregation and biofilm formation...
November 2016: Biofouling
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