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D Schneidereit, H Vass, B Reischl, R J Allen, O Friedrich
The fluorescent Ca2+ sensitive dyes Fura Red (ratiometric) and Fluo-4 (non-ratiometric) are widely utilized for the optical assessment of Ca2+ fluctuations in vitro as well as in situ. The fluorescent behavior of these dyes is strongly depends on temperature, pH, ionic strength and pressure. It is crucial to understand the response of these dyes to pressure when applying calcium imaging technologies in the field of high pressure bioscience. Therefore, we use an optically accessible pressure vessel to pressurize physiological Ca2+-buffered solutions at different fixed concentrations of free Ca2+ (1 nM to 25...
2016: PloS One
L L Xiao, Y Liu, S Chen, B M Fu
Adhesion of circulating tumor cells (CTCs) to the microvessel wall largely depends on the blood hydrodynamic conditions, one of which is the blood viscosity. Since blood is a non-Newtonian fluid, whose viscosity increases with hematocrit, in the microvessels at low shear rate. In this study, the effects of hematocrit, vessel size, flow rate and red blood cell (RBC) aggregation on adhesion of a CTC in the microvessels were numerically investigated using dissipative particle dynamics. The membrane of cells was represented by a spring-based network connected by elastic springs to characterize its deformation...
October 13, 2016: Biomechanics and Modeling in Mechanobiology
T Vuk, J Bingulac-Popović, T Očić, L J Mayer, M Milošević, I Jukić
OBJECTIVES: The aim of this study was to assess the appropriateness of using combined cell index (CCI) in the assessment of iron stores in blood donors. This index is calculated by the formula: red blood cell distribution width (RDW) × 10(4) × mean corpuscular volume (MCV)(-1) × mean corpuscular haemoglobin (MCH)(-1) . BACKGROUND: Ferritin measurement is a reliable method for estimating iron stores in blood donors. The sensitivity of red blood cell (RBC) parameters of complete blood count in detecting non-anaemic iron deficiency is significantly lower...
October 11, 2016: Transfusion Medicine
(no author information available yet)
This book is an informative reference guide to pathologies, illnesses and some minor injuries that nurse practitioners, paramedics, doctors and allied health professionals are likely to deal with in their daily practice. Laid out in a logical way, its outlines red flags, risk factors, causes, assessment, diagnostics, and management of various conditions.
October 6, 2016: Emergency Nurse: the Journal of the RCN Accident and Emergency Nursing Association
Melanka Brackx, Shari Van Wittenberghe, Jolien Verhelst, Paul Scheunders, Roeland Samson
In urban areas, the demand for local assessment of air quality is high. The existing monitoring stations cannot fulfill the needs. This study assesses the potential of hyperspectral tree leaf reflectance for monitoring traffic related air pollution. Hereto, 29 Carpinus betulus saplings were exposed to an environment with either high or low traffic intensity. The local air quality was estimated by leaf saturation isothermal remanent magnetization (SIRM). The VIS-NIR leaf reflectance spectrum (350-2500 nm) was measured using a handheld AgriSpec spectroradiometer (ASD Inc...
October 6, 2016: Environmental Pollution
Tao Su, Wei-Wei Zhang, Ya-Ming Zhang, Brian Chi-Yan Cheng, Xiu-Qiong Fu, Ting Li, Hui Guo, Ya-Xi Li, Pei-Li Zhu, Hui Cao, Zhi-Ling Yu
ETHNOPHARMACOLOGICAL RELEVANCE: Pinelliae Rhizoma (PR), the dried tuber of Pinellia ternata (Thunb.) Breit., is a traditional Chinese medicinal herb. It is commonly used for treating cancer, cough and phlegm. To treat cancer, Chinese medicine practitioners often use raw PR; while to treat cough and phlegm, they usually use Pinelliae Rhizoma Praeparatum cum Zingibere et Al..umine (PRZA, raw PR processed with ginger juice and alumen as adjuvant materials). Currently, the producing protocol of PRZA varies greatly among different places in China...
October 4, 2016: Journal of Ethnopharmacology
Louise Man, H Raymond Tahhan
A current focus of transfusion medicine is a judicious strategy in transfusion of blood products. Unfortunately, our ability to predict hemoglobin (Hgb) response to transfusion has been limited. The objective of this study was to determine variability of response to red blood cell transfusion and to predict which patients will have an Hgb rise higher or lower than that predicted by the long-standing convention of "one and three". This was a retrospective chart review in a single hospital. Data for 167 consecutive patient encounters were reviewed...
2016: Journal of Blood Medicine
Satendra Pal Singh, Minseuk Kim, Woon Bae Park, Jin-Woong Lee, Kee-Sun Sohn
A solid-state combinatorial chemistry approach, which used the A-Ge-O (A = Li, K, Rb) system doped with a small amount of Mn(4+) as an activator, was adopted in a search for novel red-emitting phosphors. The A site may have been composed of either a single alkali metal ion or of a combination of them. This approach led to the discovery of a novel phosphor in the above system with the chemical formula Li3RbGe8O18:Mn(4+). The crystal structure of this novel phosphor was solved via direct methods, and subsequent Rietveld refinement revealed a trigonal structure in the P3̅1m space group...
October 17, 2016: Inorganic Chemistry
Jessica M Lipschitz, Andrea L Paiva, Colleen A Redding, Deborah Levesque, Joseph S Rossi, Risa B Weisberg, James O Prochaska
PURPOSE: Anxiety is the most common and costly mental illness in the United States. Reducing avoidance is a core element of evidence-based treatments. Past research shows readiness to address avoidance affects outcomes. Investigating avoidance from a transtheoretical model (TTM) perspective could facilitate tailored approaches for individuals with low readiness. This study developed and examined psychometric properties of TTM measures for addressing anxiety-based avoidance. DESIGN: Cross-sectional survey...
September 26, 2016: American Journal of Health Promotion: AJHP
A R Markande, A S Nerurkar
A novel estuarine bacterial strain, Solibacillus silvestris AM1, produces an extracellular, thermostable and fibrous, glycoprotein bioemulsifier (BE-AM1). The amyloid nature of the bioemulsifier (BE-AM1) was confirmed by biophysical techniques (Congo red based polarization microscopy, ThioflavinS based fluorescent microscopy, fibrous arrangement in transmission electron microscopy and secondary structure measurement by FTIR and CD spectrum analysis). Cell-bound BE-AM1 production by S. silvestris AM1 during the mid-logarithmic phase of growth coincided with a decrease in cell surface hydrophobicity, and an increase in cell autoaggregation and biofilm formation...
November 2016: Biofouling
Hongwei Hu, Teddy Salim, Bingbing Chen, Yeng Ming Lam
Organic-inorganic hybrid perovskites have the potential to be used as a new class of emitters with tunable emission, high color purity and good ease of fabrication. Recent studies have so far been focused on three-dimensional (3D) perovskites, such as CH3NH3PbBr3 and CH3NH3PbI3 for green and infrared emission. Here, we explore a new series of hybrid perovskite emitters with a general formula of (C4H9NH3)2(CH3NH3)n-1PbnI3n+1 (where n = 1, 2, 3), which possesses a multiple quantum well structure. The quantum well thickness of these materials is adjustable through simple molecular engineering which results in a continuously tunable bandgap and emission spectra...
2016: Scientific Reports
Ki-Shuk Shim, Chung-Jo Lee, Nam-Hui Yim, Min Jung Gu, Jin Yeul Ma
Alpinia officinarum rhizome has been used as a traditional herbal remedy to treat inflammatory and internal diseases. Based on the previously observed inhibitory effect of A. officinarum rhizome in an arthritis model, we evaluated whether a water extract of A. officinarum rhizome (WEAO) would enhance in vitro osteoblast mineralization using calvarial osteoblast precursor cells or would inhibit in vitro osteoclast differentiation and bone resorption using bone marrow derived macrophages. In osteoblasts, WEAO enhanced the mRNA levels of transcription factor (runt-related transcription factor 2, smad1, smad5, and junB) and marker (bone morphogenetic protein-2, collagen type 1alpha1, and osteocalcin) genes related to osteoblast mineralization, consistent with increased alizarin red S staining intensity...
2016: American Journal of Chinese Medicine
Liying Tang, Fengli Yang, Zhirui Fang, Chengmu Hu
Alcoholic fatty liver (AFL) is early stage of alcoholic liver disease, which can progress to steatohepatitis, fibrosis, and cirrhosis if alcohol consumption is continued. The pathogenesis of AFL is associated with excessive lipid accumulation in hepatocytes. Resveratrol (RES), a dietary polyphenol found in red wines and grapes, has been shown to have a hepatoprotective effect. Autophagy is a crucial physiological process in cellular catabolism that involves the regulation of lipid droplets. Autophagy maintains a balance between protein synthesis, degradation and self-recycling...
2016: American Journal of Chinese Medicine
J B Prasanna Kumar, G Ramgopal, D V Sunitha, B Daruka Prasad, H Nagabhushana, Y S Vidya, K S Anantharaju, S C Prashantha, S C Sharma, K R Prabhakara
Cr(3) (+) -doped Y2 O3 (0.5-9 mol%) was synthesized by a simple solution combustion method using Aloe vera gel as a fuel/surfactant. The final obtained product was calcined at 750°C for 3 h, which is the lowest temperature reported so far for the synthesis of this compound. The calcined product was confirmed for its crystallinity and purity by powder X-ray diffraction (PXRD) studies which showed a single-phase nano cubic phosphor. The particles size estimated by Scherrer formula was in the range of 6-19 nm...
September 12, 2016: Luminescence: the Journal of Biological and Chemical Luminescence
Evgeniya Levy, Gregory Barshtein, Leonid Livshits, Paul Ben Ishai, Yuri Feldman
The vitality of red blood cells depends on the process control of glucose homeostasis, including the membrane's ability to "switch off" d-glucose uptake at the physiologically specific concentration of 10-12 mM. We present a comprehensive study of human erythrocytes suspended in buffer solutions with varying concentrations of d-glucose at room temperature, using microwave dielectric spectroscopy (0.5 GHz-50 GHz) and cell deformability characterization (the Elongation ratio). By use of mixture formulas the contribution of the cytoplasm to the dielectric spectra was isolated...
October 6, 2016: Journal of Physical Chemistry. B
Liangliang Zhang, Jiahua Zhang, Guo-Hui Pan, Xia Zhang, Zhendong Hao, Yongshi Luo, Huajun Wu
Luminescence property of low-concentration Eu(2+)-doped SrAlSi4N7:Ce(3+) yellow phosphor is reported in this paper. Three optical centers Ce1, Ce2, and Eu2 are observed in the phosphor. Deconvolution of emission spectrum confirms the three centers to be green (530 nm), yellow (580 nm), and red (630 nm), respectively. This property promises considerable improvement of color-rendering property of a white light-emitting diode (wLED). For example, color-rendering index (CRI) of wLED fabricated by combining a blue LED chip and SrAlSi4N7:0...
October 3, 2016: Inorganic Chemistry
Houcine Labiadh, Karima Lahbib, Slah Hidouri, Soufiane Touil, Tahar Ben Chaabane
OBJECTIVES: To evaluate the contributions of the some quantum dots in different biological uses in order to valorizes such nanomaterials for further applications. METHODS: Zinc sulfide ZnS nanoparticles were synthesized in aqueous medium at pH constant, the obtained nanoparticles has been characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier Transform Infra-red (FTIR) spectroscopies. Zinc sulfide nanoparticles were screened for their antibacterial and antifungal profiling and tested for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (OH) and hydrogen peroxide (H2O2) scavenging activity, ferric reducing power (FRP) assay and ferrous ion chelating (FIC) methods...
August 2016: Asian Pacific Journal of Tropical Medicine
Xing Guo, Qi Fang, Chendong Ma, Bangyuan Zhou, Yi Wan, Runshen Jiang
BACKGROUND: Selective breeding for genetic improvement is expected to leave distinctive selection signatures within genomes. The identification of selection signatures can help to elucidate the mechanisms of selection and accelerate genetic improvement. Fighting chickens have undergone extensive artificial selection, resulting in modifications to their morphology, physiology and behavior compared to wild species. Comparing the genomes of fighting chickens and wild species offers a unique opportunity for identifying signatures of artificial selection...
2016: Genetics, Selection, Evolution: GSE
Samer Al-Samir, Dominique Goossens, Jean-Pierre Cartron, Søren Nielsen, Frank Scherbarth, Stephan Steinlechner, Gerolf Gros, Volker Endeward
We have measured maximal oxygen consumption ([Formula: see text]O2,max) of mice lacking one or two of the established mouse red-cell CO2 channels AQP1, AQP9, and Rhag. We intended to study whether these proteins, by acting as channels for O2, determine O2 exchange in the lung and in the periphery. We found that [Formula: see text]O2,max as determined by the Helox technique is reduced by ~16%, when AQP1 is knocked out, but not when AQP9 or Rhag are lacking. This figure holds for animals respiring normoxic as well as hypoxic gas mixtures...
2016: Frontiers in Physiology
Maria Pilarska, Monika Wiciarz, Ivan Jajić, Małgorzata Kozieradzka-Kiszkurno, Petre Dobrev, Radomíra Vanková, Ewa Niewiadomska
Isolated thylakoids from halophytic Eutrema salsugineum (Thellungiella salsuginea) produces more H2O2 in comparison to glycophytic Arabidopsis thaliana. The first objective of this study was to verify whether this feature is relevant also to the intact chloroplasts and leaves. Enhanced H2O2 levels in chloroplasts and leaves of E. salsugineum were positively verified with several methods (electron microscopy, staining with Amplex Red and with diaminobenzidine). This effect was associated with a decreased ratio of [Formula: see text]/H2O2 in E...
2016: Frontiers in Plant Science
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