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https://www.readbyqxmd.com/read/29240843/behavior-of-bacteriome-symbionts-during-transovarial-transmission-and-development-of-the-asian-citrus-psyllid
#1
Hiroki Dan, Naoya Ikeda, Masaya Fujikami, Atsushi Nakabachi
The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a serious pest worldwide, transmitting Candidatus Liberibacter spp. (Alphaproteobacteria), the causative agents of a devastating citrus disease known as huanglongbing or greening disease. In a symbiotic organ called the bacteriome, D. citri possesses an organelle-like defensive symbiont, Candidatus Profftella armatura (Betaproteobacteria), and a nutritional symbiont, Ca. Carsonella ruddii (Gammaproteobacteria). Drastically reduced symbiont genomes and metabolic complementarity among the symbionts and D...
2017: PloS One
https://www.readbyqxmd.com/read/29239288/metaxonchium-toroense-n-sp-nematoda-dorylaimida-belondiridae-from-costa-rica-with-the-first-molecular-study-of-a-representative-of-the-genus
#2
I Varela-Benavides, R Peña-Santiago
The new species Metaxonchium toroense n. sp. from natural habitats of Costa Rica is described, including light microscopy (LM), scanning electron microscopy (SEM) and molecular (D2-D3 28S rDNA) analyses. The new species is characterized by its general size, the dimensions and appearance of its lip region, the length of the odontostyle and its fusiform aspect, the length of the neck and its pharyngeal expansion, the reduction of the anterior genital branch to a very short uterine sac without any rudiment of ovary or oviduct, tripartite and non-echinophor posterior uterus, the somewhat posterior vulva position, the length and shape of the caudal region, and the absence of males...
December 14, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/29239046/sperm-preparation-through-sephadex%C3%A2-filtration-improves-in-vitro-fertilization-rate-of-buffalo-oocytes
#3
A U Husna, A Azam, S Qadeer, M A Awan, S Nasreen, Q Shahzad, A Fouladi-Nashta, M Khalid, S Akhter
Routinely, swim-up method is used to separate high-quality sperm; however, long processing time and close cell-to-cell contact during the centrifugation step are inevitable elements of oxidative stress to sperm. The objective was to evaluate Sephadex™ and glass wool filtration to separate motile, intact and viable sperm for in vitro fertilization in buffalo. The cumulus-oocyte complexes (COCs) were collected from ovaries of slaughtered buffaloes by aspiration and matured for 24 hr in CO2 incubator at 38...
December 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29238873/insights-into-the-generation-of-monoclonal-antibody-acidic-charge-variants-during-chinese-hamster-ovary-cell-cultures
#4
Hongping Tang, Shiwei Miao, Xintao Zhang, Li Fan, Xuping Liu, Wen-Song Tan, Liang Zhao
Charge variation is one of the most important heterogeneities during monoclonal antibody (mAb) manufacturing and this study presents insights into the generation of acidic charge variants during cell culture processes. Since acidic variants generate both intracellularly and extracellularly, main charge fraction collected by weak cation exchange chromatography (WCX) was incubated in harvested cell supernatant (HCS) to simulate and investigate the extracellular process firstly. It is found that the main fraction was degraded rapidly into acidic variants rather than basic variants extracellularly, and the degradation sites were located in both Fab and Fc fragments indicated by papain digestion...
December 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29238579/acute-abdomen-with-a-misleading-clinical-entity
#5
Jitendra Singh Nigam, Pushpanjali Ojha, Chitrawati Bal Gargade, Archana Hemant Deshpande
Massive ovarian edema is a rare, non-neoplastic solid tumor-like lesion. It results from compromised venous and lymphatic drainage due to partial or intermittent torsion of ovarian pedicle. Pain, distension or abdominal mass, menstrual irregularities, infertility and hormone-related symptoms can be the clinical presentation. We report a case of massive ovarian edema in a 28-year-old female who presented with acute pain abdomen. She was diagnosed clinico-radiologically with solid ovarian tumor and massive ovarian edema on histology...
2017: SAGE Open Medical Case Reports
https://www.readbyqxmd.com/read/29238465/genistein-preserves-the-lungs-of-ovariectomized-diabetic-rats-addition-to-apoptotic-and-inflammatory-markers-in-the-lung
#6
Faeze Daghigh, Alireza Alihemmati, Pouran Karimi, Parisa Habibi, Naser Ahmadiasl
Objectives: The role of isoflavones in pulmonary structure and function during menopause is not well studied. Moreover, the important role of estrogen in the physiological function of respiratory system has been revealed. Genistein, as an isoflavone, mimics estrogenic in diabetic and ovariectomized rats. Here, we hypothesized that genistein would reverse changes in the protein expression levels related to estrogen deficiency in the lung of ovariectomized diabetic rats. Materials and Methods: Wistar female rats were assigned to four experimental groups (n=10 in each group): sham, rats underwent laparotomy without removing the ovaries; OVX, rats that underwent ovariectomy; OVX...
December 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29238344/scrambled-eggs-apoptotic-cell-clearance-by-non-professional-phagocytes-in-the-drosophila-ovary
#7
REVIEW
Sandy B Serizier, Kimberly McCall
For half of a century, it has been known that non-professional phagocytes, such as fibroblasts, endothelial, and epithelial cells, are capable of efferocytosis (engulfment of apoptotic cells). Non-professional phagocytes differ from professional phagocytes in the range and efficiency of engulfment. Much of the recognition and underlying signaling machinery between non-professional and professional phagocytes is the same, but it is not known how the engulfment capacity of non-professional phagocytes is controlled...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29237855/sex-specific-adaptation-and-genomic-responses-to-y-chromosome-presence-in-female-reproductive-and-neural-tissues
#8
Alan T Branco, Rute M Brito, Bernardo Lemos
Y chromosomes typically harbour a small number of genes and an abundance of repetitive sequences. In Drosophila, the Y chromosome comprises multimegabase long segments of repetitive DNA and a handful of protein-coding genes. In mammals, the Y chromosome also harbours a disproportionally high abundance of repeats. Here, we built on a Drosophila melanogaster model in which the Y chromosome is decoupled from sexual determination. Genotypes were genetically identical for the autosomes, X chromosome, and mitochondria, but differ by the presence or dose of the Y chromosome...
December 20, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29237725/abin-1-negatively-regulates-%C3%AE-opioid-receptor-function
#9
Peilan Zhou, Jiebing Jiang, Hui Yan, Yulei Li, Junru Zhao, Xiao Wang, Ruibin Su, Zehui Gong
The μ-opioid receptor (MOR) is a Gi/o protein-coupled receptor that mediates analgesic, euphoric, and reward effects. Using a bacterial two-hybrid screen, we reported that the carboxyl tail of the rat MOR associates with A20-binding inhibitor of nuclear factor (NF)-κB (ABIN-1) (Zhou et al., 2015). This interaction was confirmed by direct protein - protein binding and co-immunoprecipitation of MOR and ABIN-1 proteins in cell lysates. Saturation binding studies showed that ABIN-1 had no effect on MOR binding...
December 13, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/29237393/micrornas-downregulation-in-cancer-is-associated-with-guanine-enrichment-in-the-terminal-loop-sequences-of-their-precursors
#10
Amit Cohen, Mario Alberto Burgos-Aceves, Yoav Smith
A global repression in microRNA (miRNA) expression is a phenomenon observed in different types of cancer. Herein, we show an association of miRNAs downregulation in cancer, with guanine (G) enrichment in the terminal loop sequences of their precursors. High G (and GG) content was mostly found in repressed miRNAs of breast, lung and ovary cancers, predominantly in poorly differentiated tumors. The mature sequences of repressed miRNAs had significantly low G level and were enriched with an ACA motif. These results suggest a new link between G enrichment of precursor miRNAs terminal loops and carcinogenesis, and the possible association of specific sequence motifs with the regulation of their expression...
December 13, 2017: MicroRNA
https://www.readbyqxmd.com/read/29237021/human-specific-subcellular-compartmentalization-of-p-element-induced-wimpy-testis-like-piwil-granules-during-germ-cell-development-and-spermatogenesis
#11
Maria Gomes Fernandes, Nannan He, Fang Wang, Liesbeth Van Iperen, Cristina Eguizabal, Roberto Matorras, Bernard A J Roelen, Susana M Chuva De Sousa Lopes
STUDY QUESTION: What is the dynamics of expression of P-element induced wimpy testis-like (PIWIL) proteins in the germline during human fetal development and spermatogenesis? SUMMARY ANSWER: PIWIL1, PIWIL2, PIWIL3 and PIWIL4 were expressed in a sex-specific fashion in human germ cells (GC) during development and adulthood. PIWILs showed a mutually exclusive pattern of subcellular localization. PIWILs were present in the intermitochondrial cement and a single large granule in meiotic GC and their expression was different from that observed in mice, highlighting species-differences...
December 11, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29236298/leptin-a-new-therapeutic-target-for-treatment-of-diabetes-mellitus
#12
Kanwal Rehman, Muhammad Sajid Hamid Akash, Zunaira Alina
Leptin is an endogenous protein having 167 amino acids and is derived from adipocytes. It hass tertiary structure that resembles with that of the pro-inflammatory cytokines family. The fundamental role of leptin is to maintain the energy homeostasis with the aid of its counter hormone called ghrelin, known as the "hunger hormone". Small quantities of leptin are also present in various tissues like ovary, placenta, pituitary gland, mammary gland, skeletal muscle, stomach and lymphoid tissue. Expression of leptin is strongly associated with various inflammatory responses and immune system, and plays crucial role in the pathophysiology of obesity and development of diabetes mellitus (DM) and insulin resistance...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236219/radioresistant-sf9-insect-cells-readily-undergo-an-intrinsic-mode-of-apoptosis-in-response-to-histone-deacetylase-hdac-inhibition
#13
Jyoti Swaroop Kumar, Shubhankar Suman, Sudhir Chandna
Insect cell lines have been utilized as an important higher eukaryotic model system to decipher stress responses and cell death mechanisms. Lepidopteran Sf9 cells (derived from the ovaries of Spodoptera frugiperda) display nearly 100 times higher resistance to ionizing radiation in contrast to mammalian cells, which is partly contributed by an unusually high HDAC activity. However, their response to HDAC inhibition remains to be evaluated. In the present study, the effects of HDAC inhibitor (NaBt) on Sf9 cellular/nuclear morphology, cell cycle progression, DNA damage/repair, redox status, and mitochondrial perturbations were evaluated...
December 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29236205/a-novel-fibrin-based-artificial-ovary-prototype-resembling-human-ovarian-tissue-in-terms-of-architecture-and-rigidity
#14
Maria Costanza Chiti, Marie-Madeleine Dolmans, Lucie Mortiaux, Flanco Zhuge, Emna Ouni, Parinaz Asiabi Kohneh Shahri, Evelyne Van Ruymbeke, Sophie-Demoustier Champagne, Jacques Donnez, Christiani Andrade Amorim
PURPOSE: The aim of this study is to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. METHODS: Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75), rheology of fibrin matrices and histology of isolated and encapsulated human ovarian follicles in these matrices. RESULTS: Fresh human ovarian cortex showed a highly fibrous and structurally inhomogeneous architecture in three age-related patients, but the mean ± SD of fiber thickness (61...
December 13, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29236054/genome-wide-profiling-of-small-rnas-and-degradome-revealed-conserved-regulations-of-mirnas-on-auxin-responsive-genes-during-fruit-enlargement-in-peaches
#15
Mengya Shi, Xiao Hu, Yu Wei, Xu Hou, Xue Yuan, Jun Liu, Yueping Liu
Auxin has long been known as a critical phytohormone that regulates fruit development in plants. However, due to the lack of an enlarged ovary wall in the model plants Arabidopsis and rice, the molecular regulatory mechanisms of fruit division and enlargement remain unclear. In this study, we performed small RNA sequencing and degradome sequencing analyses to systematically explore post-transcriptional regulation in the mesocarp at the hard core stage following treatment of the peach (Prunus persica L.) fruit with the synthetic auxin α-naphthylacetic acid (NAA)...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235035/is-acupuncture-actually-not-effective-for-polycystic-ovary-syndrome-analysis-of-the-trial-published-in-jama
#16
Wei-Juan Gang, Xiang-Hong Jing
In recent years, acupuncture has been applied to treat more and more patients suffering from polycystic ovary syndrome (PCOS). On June 27, 2017 a paper titled "Effect of acupuncture and clomiphene in Chinese women with polycystic ovary syndrome: a randomized clinical trial" was published in Journal of the American Medical Association (JAMA). The results did not support acupuncture as an effective therapy for improving the live births in PCOS. The aim of this article was to analyze the therapeutic regimen and design of the clinical trial...
December 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29233731/do-different-diagnostic-criteria-cause-impact-polycystic-ovary-syndrome-diagnosis-for-adolescents
#17
Sinem Akgül, Yasemin Düzçeker, Nuray Kanbur, Orhan Derman
OBJECTIVE: While early diagnosis of polycystic ovary syndrome (PCOS) in adolescents may allow for earlier treatment and prevention of chronic disorders, incorrect or premature diagnosis carries risks of unnecessary treatment and psychological distress. There is no consensus concerning which diagnostic criteria to use for adolescents and current criteria vary. The objective of this study was to determine whether using different diagnostic criteria will affect PCOS diagnosis in adolescents...
December 9, 2017: Journal of Pediatric and Adolescent Gynecology
https://www.readbyqxmd.com/read/29233547/the-developing-spectrum-of-gastric-type-cervical-glandular-lesions
#18
REVIEW
Karen L Talia, W Glenn McCluggage
Adenocarcinoma of the cervix is less common than squamous cell carcinoma, although its relative prevalence is increasing. Oncogenic (high-risk) human papillomavirus (HPV) infection is implicated in the development of approximately 90% of all cervical adenocarcinomas. Of the remaining non-HPV associated tumours, the most frequent is gastric-type adenocarcinoma (GAS), which is recognised by the World Health Organization as a form of mucinous adenocarcinoma of the cervix. Minimal deviation adenocarcinoma (MDA) of mucinous type (adenoma malignum) is considered an extremely well differentiated variant of GAS and is encompassed within the category of GAS...
December 9, 2017: Pathology
https://www.readbyqxmd.com/read/29233485/body-fat-affects-mouse-reproduction-ovarian-hormone-release-and-response-to-follicular-stimulating-hormone
#19
Alexander V Sirotkin, Dušan Fabian, Janka Babeľová Kubandová, Radoslava Vlčková, Saleh Alwasel, Abdel Halim Harrath
We investigated the effects of body fat content on mouse fecundity, ovarian hormone release, and their response to follicle stimulation hormone (FSH). 4 types of females were produced: lean (group 1), normal (group 2), slightly fat (group 3), and significantly fat (group 4). The body weights, fat content, fertility rate, embryo number produced, retarded and degenerated embryo percentage, the release of progesterone (P4), testosterone (T), and insulin-like growth factor I (IGF-I) by isolated ovaries cultured with and without FSH (1...
December 7, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29233458/development-of-hyper-osmotic-resistant-cho-host-cells-for-enhanced-antibody-production
#20
Yasuharu Kamachi, Takeshi Omasa
Cell culture platform processes are generally employed to shorten the duration of new product development. A fed-batch process with continuous feeding is a conventional platform process for monoclonal antibody production using Chinese hamster ovary (CHO) cells. To establish a simplified platform process, the feeding method can be changed from continuous feed to bolus feed. However, this change induces a rapid increase of osmolality by the bolus addition of nutrients. The increased osmolality suppresses cell culture growth, and the final product concentration is decreased...
December 7, 2017: Journal of Bioscience and Bioengineering
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