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ion exchange resin

Mariam S Aboian, Jay F Yu, Ayushi Gautam, Chia-Hung Sze, Jeffrey K Yang, Jonathan Chan, Prasheel V Lillaney, Caroline D Jordan, Hee-Jeung Oh, David M Wilson, Anand S Patel, Mark W Wilson, Steven W Hetts
To report a novel method using immobilized DNA within mesh to sequester drugs that have intrinsic DNA binding characteristics directly from flowing blood. DNA binding experiments were carried out in vitro with doxorubicin in saline (PBS solution), porcine serum, and porcine blood. Genomic DNA was used to identify the concentration of DNA that shows optimum binding clearance of doxorubicin from solution. Doxorubicin binding kinetics by DNA enclosed within porous mesh bags was evaluated. Flow model simulating blood flow in the inferior vena cava was used to determine in vitro binding kinetics between doxorubicin and DNA...
December 2016: Biomedical Microdevices
Alessandra Imbrogno, Jennifer Biscarat, Andrea Iris Schäfer
BACKGROUND: Estrogens and their synthetic analogues are widely used as pharmaceuticals. Upon oral administration these drugs are eventually excreted via urine. The persistence of these pharmaceuticals and inefficient removal by water treatment lead to accumulation in surface water and effluents with negative effects for aquatic life and human health. METHODS: In this study, the uptake of estradiol by a combined magnetic ion exchange resin - ultrafiltration process (MIEX-UF) was investigated...
October 21, 2016: Current Pharmaceutical Design
Xavier LeSaout, Matteo Costioli, Lynn Jordan, Jeremy Lambert, Ross Beighley, Laurel Provencher, Brian Gerwe, Kevin McGuire, Nico Verlinden, Andrew Barry
Small-scale protein purification presents opportunities for accelerated process development of biotherapeutic molecules. Miniaturization of purification conditions reduces time and allows for parallel processing of samples, thus offering increased statistical significance and greater breadth of variables. The ability of the miniaturized platform to be predictive of larger scale purification schemes is of critical importance. The PerkinElmer JANUS BioTx Pro and Pro-Plus workstations were developed as intuitive, flexible, and automated devices capable of performing parallel small-scale analytical protein purification...
March 2016: Engineering in Life Sciences
Ronivaldo Rodrigues da Silva, Lilian Caroline Gonçalves de Oliveira, Maria Aparecida Juliano, Luiz Juliano, Jose C Rosa, Hamilton Cabral
Peptidases are enzymes that catalyze the rupture of peptide bonds. Catalytic specificity studies of these enzymes have illuminated their modes of action and preferred hydrolysis targets. We describe the biochemical characteristics and catalytic specificity of a lysine-dependent peptidase secreted by the basidiomycete fungus Phanerochaete chrysosporium. We attained 5.7-fold purification of a ∼23-kDa neutral peptidase using size-exclusion (Sephadex G-50 resin) and ion-exchange (Source 15S resin) chromatography...
October 19, 2016: International Journal of Biological Macromolecules
R Kelman Wieder, Melanie A Vile, Kimberli D Scott, Cara M Albright, Kelly McMillen, Dale H Vitt, Mark Fenn
Oil extraction and development activities in the Athabasca Oil Sands Region of northern Alberta, Canada release NOx, SOx, and NHx to the atmosphere, ultimately resulting in increasing N and S inputs to surrounding ecosystems through atmospheric deposition. Peatlands are a major feature of the northern Alberta landscape, with bogs covering 6-10% of the land area, and fens covering 21-53%. Bulk deposition of NH4+-N, NO3--N, total inorganic N (TIN), and SO42--S, was quantified using ion exchange resin collectors deployed at 23 locations, over 1-6 years, reveal maximum N and S deposition of 9...
October 21, 2016: Environmental Science & Technology
Robert Hunt Dunlap, Ryan Martinez
Kayexalate is an ion exchange resin that is commonly used to acutely treat patients with hyperkalemia. Bowel ulceration and necrosis is a rare and uncommonly recognized complication of kayexalate administration. More often, concomitant administration with sorbitol is reported to damage the bowel; however, there are reports of kayexalate administration causing bowel necrosis without sorbitol. We present a case of a critically ill patient who underwent total colectomy for colonic necrosis secondary to oral kayexalate administration that was not recognized until late in the pathologic process...
October 7, 2016: Journal of Surgical Case Reports
Tamara Markovic, Saba Manzoor, Emma Humphreys-Williams, Guy Kirk, Ramon Vilar, Dominik Jakob Weiss
The stable isotope signatures of zinc are increasingly used to study plant and soil processes. Complexation with phytosiderophores is a key process and understanding the controls of isotope fractionation is central to such studies. Here, we investigated isotope fractionation during complexation of Zn2+ with the phytosiderophore 2'-deoxymugeneic acid (DMA) - which we synthesised - and with three commercially-available structural analogues of DMA: EDTA, TmDTA and CyDTA. We used ion exchange chromatography to separate free and complexed zinc, and identified appropriate cation exchange resins for the individual systems...
October 17, 2016: Environmental Science & Technology
Gregory D Tredwell, Jacob G Bundy, Maria De Iorio, Timothy M D Ebbels
INTRODUCTION: Despite the use of buffering agents the (1)H NMR spectra of biofluid samples in metabolic profiling investigations typically suffer from extensive peak frequency shifting between spectra. These chemical shift changes are mainly due to differences in pH and divalent metal ion concentrations between the samples. This frequency shifting results in a correspondence problem: it can be hard to register the same peak as belonging to the same molecule across multiple samples. The problem is especially acute for urine, which can have a wide range of ionic concentrations between different samples...
2016: Metabolomics: Official Journal of the Metabolomic Society
Akihide Yoshihara, Taro Kozakai, Tomoya Shintani, Ryo Matsutani, Kouhei Ohtani, Tetsuo Iida, Kazuya Akimitsu, Ken Izumori, Pushpa Kiran Gullapalli
An enzyme that catalyzes C-3 epimerization between d-fructose and d-allulose was found in Arthrobacter globiformis strain M30. Arthrobacter species have long been used in the food industry and are well-known for their high degree of safety. The enzyme was purified by ion exchange and hydrophobic interaction chromatographies and characterized as a d-allulose 3-epimerase (d-AE). The molecular weight of the purified enzyme was estimated to be 128 kDa with four identical subunits. The enzyme showed maximal activity and thermostability in the presence of Mg(2+)...
October 3, 2016: Journal of Bioscience and Bioengineering
Jinze Li, Suman Koner, Michael German, Arup K Sengupta
From a sustainability viewpoint, sodium exchange softening, although used widely, is under scrutiny due to its production of excess Na-laden spent regenerant and subsequent discharge to the environment. Many arid regions are introducing regulations disallowing dumping of concentrated sodium salts, the residuals from popular Na-exchange softening. The sodium content of the softened water is, also, always higher than in the feed, which poses a dietary health concern when used for drinking or cooking. An efficient, easy-to-operate hardness removal process with reduced sodium in both the treated water and in the spent regenerant is an unmet global need...
October 4, 2016: Environmental Science & Technology
Latchezar I Tsonev, Allen G Hirsh
We have previously described a liquid chromatographic (LC) method for uncoupling controlled, wide range pH gradients and simultaneous controlled gradients of a non-buffering solute on ion exchange resins (Hirsh and Tsonev, 2012) [1]. Here we report the application of this two dimensional LC technique to the problem of resolving Human Transferrin (HT) isoforms. This important iron transporting protein should theoretically occur in several thousand glycoforms, but only about a dozen have been reported. Using dual simultaneous independent gradients (DSIGs) of acetonitrile (ACN) and pH on a mixed bed stationary phase (SP) consisting of a mixture of an anion exchange resin and a reversed phase (RP) resin we partially resolve about 60 isoforms...
October 14, 2016: Journal of Chromatography. A
Marina Palamarchuk, Andrey Egorin, Eduard Tokar, Mikhail Tutov, Dmitry Marinin, Valentin Avramenko
The origin of the emergence of radioactive contamination not removable in the process of acid-base regeneration of ion-exchange resins used in treatment of technological media and liquid radioactive waste streams has been determined. It has been shown that a majority of cesium radionuclides not removable by regeneration are bound to inorganic deposits on the surface and inside the ion-exchange resin beads. The nature of the above inorganic inclusions has been investigated by means of the methods of electron microscopy, IR spectrometry and X-ray diffraction...
September 4, 2016: Journal of Hazardous Materials
Amanda Alonso, Núria Vigués, Rosalía Rodríguez-Rodríguez, Xavier Borrisé, María Muñoz, Dmitri N Muraviev, Jordi Mas, Xavier Muñoz-Berbel
This paper presents the study of the dynamics of the formation of polymer-assisted highly-orientated polycrystalline cubic structures (CS) by a fractal-mediated mechanism. This mechanism involves the formation of seed Ag@Co nanoparticles by InterMatrix Synthesis and subsequent overgrowth after incubation at a low temperature in chloride and phosphate solutions. These ions promote the dissolution and recrystallization in an ordered configuration of pre-synthetized nanoparticles initially embedded in negatively-charged polymeric matrices...
October 21, 2016: Nanotechnology
Yanliang Chen, Yuezhou Wei, Linfeng He, Fangdong Tang
To separate thorium and uranium in nitric acid solution using anion exchange process, a strong base silica-based anion exchange resin (SiPyR-N4) was synthesized. Batch experiments were conducted and the separation factor of thorium and uranium in 9M nitric acid was about 10. Ion exchange chromatography was applied to separate thorium and uranium in different ratios. Uranium could be eluted by 9M nitric acid and thorium was eluted by 0.1M nitric acid. It was proved that thorium and uranium can be separated and recovered successfully by this method...
September 30, 2016: Journal of Chromatography. A
Kenneth Reed, Amy Li, Britney Wilson, Tetchi Assamoi
PURPOSE: The purpose of this study was to determine if the addition of calcium gluconate to gellan solution results in a stronger gel structure on initial exposure to tear fluid due to the displacement of calcium from the gluconate ion by tear monovalent cations (Na(+), K(+)). METHODS: Test solutions of gellan and gellan-calcium gluconate were mixed thoroughly with simulated tear fluid (STF) at a 5:1 ratio. The resulting gel was measured for viscosity at 32°C-36°C...
September 9, 2016: Journal of Ocular Pharmacology and Therapeutics
A Michael Rajesh, Kiritkumar Mangaldas Popat
OBJECTIVE: The objective was to evaluate taste masking of azithromycin (AZI) by ion exchange resins (IERs) and the formation of covalent semi interpenetrating polymer network (IPN) beads using chitosan (CS) and sodium carboxylated agarose (SCAG) for sustained release of drug. METHODS: Methacrylic acid (MAA)-based IERs were prepared by suspension polymerization method. Drug release complexes (DRCs) were prepared by different drug:resin ratios i.e. 1:1, 1:2 and 1:4...
September 7, 2016: Drug Development and Industrial Pharmacy
Helena I Gomes, Ashley Jones, Mike Rogerson, Ian T Burke, William M Mayes
Bauxite residue is an important by-product of the alumina industry, and current management practices do not allow their full valorisation, especially with regard to the recovery of critical metals. This work aims to test the efficiency of ion exchange resins for vanadium (V) removal and recovery from bauxite residue leachates at alkaline pH (11.5 and 13). As an environmental pollutant, removal of V from leachates may be an obligation of bauxite residue disposal areas (BRDA) long-term management requirements...
September 1, 2016: Environmental Science and Pollution Research International
Tomoyuki Ohya, Kotaro Nagatsu, Hisashi Suzuki, Masami Fukada, Katsuyuki Minegishi, Masayuki Hanyu, Toshimitsu Fukumura, Ming-Rong Zhang
INTRODUCTION: Copper-64 is an attractive radionuclide for positron emission tomography and is emerging as a radiotherapeutic agent. The demand of (64)Cu with low metallic impurities has increased because of its wide applications when incorporated with antibodies, peptides, and proteins. In this study, we propose a new separation method to produce high-quality (64)Cu using a cation exchange column, as well as an automated separation system suitable for large-scale production. METHODS: (64)Cu was produced from an electrodeposited (64)Ni target via the (64)Ni(p,n)-reaction with a 24MeV HH+ beam at 10eμA (electrical microampere) conducted for 1-3h...
July 30, 2016: Nuclear Medicine and Biology
Rahul Bhambure, Christopher M Gillespie, Michael Phillips, Heiner Graalfs, Abraham M Lenhoff
The ligand density critically affects the performance of ion-exchange resins in such measures as the adsorption capacity and transport characteristics. However, for tentacular and other polymer-modified exchangers, the mechanistic basis of the effect of ligand density on performance is not yet fully understood. In this study we map the ionic strength-dependent structural changes in tentacular cation exchangers with variable ligand densities as the basis for subsequent investigation of effects on functional properties...
September 9, 2016: Journal of Chromatography. A
Andrea C Galvis-Sánchez, João Rodrigo Santos, António O S S Rangel
In this work, a total flow analysis system based on a novel solid-liquid extraction chamber is presented. This strategy enables all the main experimental procedures for the analysis of a solid sample to be performed automatically: enrichment of the liquid extract, sample treatment, filtration of the liquid extract from the solid sample, directing the extract towards detection, and finally cleansing of the chamber for the following solid sample to be analyzed. The chamber designed to be incorporated in the flow manifold presents two main features: it accommodates stirring bars for enhancing the extraction process, and it presents replaceable solid sample containers (a spare part of the solid-liquid extraction chamber) to easily replace the solid sample and therefore enhance sample analysis throughput...
November 2016: Analytical and Bioanalytical Chemistry
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